Restriction map for
pF1KA0791
(Circular) (Six-base) MAPSORT of: pF1KA0791.seq Check: 1106 from: 1 to: 7109
pF1KA0791 7109 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1012 1012
Size: 7109
AccI GT'mk_AC
Cuts at: 443 1809 3068 3909 4147 443
Size: 1366 1259 841 238 3405
Fragments arranged by size:
3405 1366 1259 841 238
Acc65I G'GTAC_C
Cuts at: 4125 4125
Size: 7109
AclI AA'CG_TT
Cuts at: 6949 6949
Size: 7109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 2248 2344 2404 2800 3247 3379 4934 5366
Size: 1571 96 60 396 447 132 1555 432
Cuts at: 5366 6113 6381 677
Size: 747 268 1405
Fragments arranged by size:
1571 1555 1405 747 447 432 396 268
132 96 60
AflIII A'CryG_T
Cuts at: 1409 3912 4461 5565 6542 1409
Size: 2503 549 1104 977 1976
Fragments arranged by size:
2503 1976 1104 977 549
AgeI A'CCGG_T
Cuts at: 5497 5497
Size: 7109
AlwNI CAG_nnn'CTG
Cuts at: 490 2163 2217 2304 4414 5981 490
Size: 1673 54 87 2110 1567 1618
Fragments arranged by size:
2110 1673 1618 1567 87 54
ApaLI G'TGCA_C
Cuts at: 5879 5879
Size: 7109
ApoI r'AATT_y
Cuts at: 605 978 1426 2086 3254 3346 3502 3689 4065
Size: 373 448 660 1168 92 156 187 376
Cuts at: 4065 4113 6462 6718 605
Size: 48 2349 256 996
Fragments arranged by size:
2349 1168 996 660 448 376 373 256
187 156 92 48
AseI AT'TA_AT
Cuts at: 20 2440 20
Size: 2420 4689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7109
AvaI C'yCGr_G
Cuts at: 1640 3287 4129 1640
Size: 1647 842 4620
Fragments arranged by size:
4620 1647 842
BamHI G'GATC_C
Cuts at: 3386 3861 4134 3386
Size: 475 273 6361
Fragments arranged by size:
6361 475 273
BanI G'GyrC_C
Cuts at: 4125 4539 4787 4822 4125
Size: 414 248 35 6412
Fragments arranged by size:
6412 414 248 35
BanII G_rGCy'C
Cuts at: 709 1164 1311 3032 3640 4022 4036 4123 5153
Size: 455 147 1721 608 382 14 87 1030
Cuts at: 5153 709
Size: 2665
Fragments arranged by size:
2665 1721 1030 608 455 382 147 87
14
BbeI G_GCGC'C
Cuts at: 4543 4791 4543
Size: 248 6861
BbsI GAAGACnn'nnnn_
Cuts at: 1568 2807 1568
Size: 1239 5870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2266 2300 2266
Size: 34 7075
BciVI GTATCCnnnnn_n'
Cuts at: 5007 5768 7109 5007
Size: 761 1341 5007
Fragments arranged by size:
5007 1341 761
BclI T'GATC_A
Cuts at: 3141 3840 4629 3141
Size: 699 789 5621
Fragments arranged by size:
5621 789 699
BfrBI ATG'CAT
Cuts at: 2852 3243 3654 2852
Size: 391 411 6307
Fragments arranged by size:
6307 411 391
BglI GCCn_nnn'nGGC
Cuts at: 652 652
Size: 7109
BglII A'GATC_T
Cuts at: 2403 4624 6661 2403
Size: 2221 2037 2851
Fragments arranged by size:
2851 2221 2037
BlpI GC'TnA_GC
Cuts at: 4221 4221
Size: 7109
Bme1580I G_kGCm'C
Cuts at: 4734 4827 5883 4734
Size: 93 1056 5960
Fragments arranged by size:
5960 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2100 2903 3777 4070 4321 4402 4737 6881 2100
Size: 803 874 293 251 81 335 2144 2328
Fragments arranged by size:
2328 2144 874 803 335 293 251 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 295 5476 211
Size: 84 5181 1844
Fragments arranged by size:
5181 1844 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 1817 2557 2918 3130 4006 512
Size: 1305 740 361 212 876 3615
Fragments arranged by size:
3615 1305 876 740 361 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3652 4285 5164 5656 5954 2741
Size: 911 633 879 492 298 3896
Fragments arranged by size:
3896 911 879 633 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 320 1733 6813 320
Size: 1413 5080 616
Fragments arranged by size:
5080 1413 616
BsaAI yAC'GTr
Cuts at: 4462 4462
Size: 7109
BsaBI GATnn'nnATC
Cuts at: 6 4623 6660 6
Size: 4617 2037 455
Fragments arranged by size:
4617 2037 455
BsaHI Gr'CG_yC
Cuts at: 4540 4788 5490 6986 4540
Size: 248 702 1496 4663
Fragments arranged by size:
4663 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4298 4819 5497 5771 5918 6329 4298
Size: 521 678 274 147 411 5078
Fragments arranged by size:
5078 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2086 2116 2086
Size: 30 7079
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 338 1426 1532 2888 3630 338
Size: 1088 106 1356 742 3817
Fragments arranged by size:
3817 1356 1088 742 106
BseYI C'CCAG_C
Cuts at: 1854 2198 3943 4534 5292 5869 6365 1854
Size: 344 1745 591 758 577 496 2598
Fragments arranged by size:
2598 1745 758 591 577 496 344
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3601 3877 2173
Size: 1428 276 5405
Fragments arranged by size:
5405 1428 276
BsiEI CG_ry'CG
Cuts at: 104 936 4651 4697 5905 104
Size: 832 3715 46 1208 1308
Fragments arranged by size:
3715 1308 1208 832 46
BsiHKAI G_wGCw'C
Cuts at: 709 1164 1845 2685 3032 3640 4022 4036 4123
Size: 455 681 840 347 608 382 14 87
Cuts at: 4123 4901 5091 5883 709
Size: 778 190 792 1935
Fragments arranged by size:
1935 840 792 778 681 608 455 382
347 190 87 14
BsmI GAATG_Cn'
Cuts at: 527 897 3656 527
Size: 370 2759 3980
Fragments arranged by size:
3980 2759 370
BsmBI CGTCTCn'nnnn_
Cuts at: 1805 1805
Size: 7109
Bsp1286I G_dGCh'C
Cuts at: 709 1164 1311 1845 2685 3032 3640 4022 4036
Size: 455 147 534 840 347 608 382 14
Cuts at: 4036 4123 4734 4827 4901 5091 5153 5883 709
Size: 87 611 93 74 190 62 730 1935
Fragments arranged by size:
1935 840 730 611 608 534 455 382
347 190 147 93 87 74 62 14
BspEI T'CCGG_A
Cuts at: 6329 6329
Size: 7109
BspHI T'CATG_A
Cuts at: 2232 3354 6285 2232
Size: 1122 2931 3056
Fragments arranged by size:
3056 2931 1122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1012 3741 4159 4373 4675 5056 1012
Size: 2729 418 214 302 381 3065
Fragments arranged by size:
3065 2729 418 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2896 5401 5455 6939 7104 50
Size: 2846 2505 54 1484 165 55
Fragments arranged by size:
2846 2505 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 917 3281 3814 5021 917
Size: 2364 533 1207 3005
Fragments arranged by size:
3005 2364 1207 533
BsrFI r'CCGG_y
Cuts at: 3556 5107 5288 5497 3556
Size: 1551 181 209 5168
Fragments arranged by size:
5168 1551 209 181
BsrGI T'GTAC_A
Cuts at: 1398 3441 3732 1398
Size: 2043 291 4775
Fragments arranged by size:
4775 2043 291
BssSI C'ACGA_G
Cuts at: 3215 5380 5738 3215
Size: 2165 358 4586
Fragments arranged by size:
4586 2165 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2443 4486 2443
Size: 2043 5066
BstBI TT'CG_AA
Cuts at: 2286 5470 2286
Size: 3184 3925
BstEII G'GTnAC_C
Cuts at: 4006 4006
Size: 7109
BstYI r'GATC_y
Cuts at: 1 537 2403 2751 3386 3861 4134 4601 4624
Size: 536 1866 348 635 475 273 467 23
Cuts at: 4624 4959 5205 6217 6303 6315 6661 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1866 1012 635 536 475 467 449 348
346 335 273 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1810 3910 1810
Size: 2100 5009
BtgI C'CryG_G
Cuts at: 1133 1133
Size: 7109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5059 5240 5403 5059
Size: 181 163 6765
Fragments arranged by size:
6765 181 163
BtsI GCAGTG_nn'
Cuts at: 238 974 1601 2015 3314 3889 4352 238
Size: 736 627 414 1299 575 463 2995
Fragments arranged by size:
2995 1299 736 627 575 463 414
ClaI AT'CG_AT
Cuts at: 932 932
Size: 7109
DraI TTT'AAA
Cuts at: 948 1358 1548 2400 3519 3956 4071 4108 948
Size: 410 190 852 1119 437 115 37 3949
Fragments arranged by size:
3949 1119 852 437 410 190 115 37
DrdI GACnn_nn'nnGTC
Cuts at: 4815 5673 4815
Size: 858 6251
EaeI y'GGCC_r
Cuts at: 3131 4694 4868 5259 5286 6631 3131
Size: 1563 174 391 27 1345 3609
Fragments arranged by size:
3609 1563 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4694 4694
Size: 7109
EarI CTCTTCn'nnn_
Cuts at: 180 557 1523 1697 4082 5132 5342 180
Size: 377 966 174 2385 1050 210 1947
Fragments arranged by size:
2385 1947 1050 966 377 210 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1685 5317 5627 5773 6919 1685
Size: 3632 310 146 1146 1875
Fragments arranged by size:
3632 1875 1146 310 146
EcoICRI GAG'CTC
Cuts at: 707 1162 3030 3638 4020 4034 4121 707
Size: 455 1868 608 382 14 87 3695
Fragments arranged by size:
3695 1868 608 455 382 87 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 677 1817 2248 2344 2404 2557 2800 2918
Size: 165 1140 431 96 60 153 243 118
Cuts at: 2918 3130 3247 3379 4006 4934 5366 6113 6381
Size: 212 117 132 627 928 432 747 268
Cuts at: 6381 512
Size: 1240
Fragments arranged by size:
1240 1140 928 747 627 432 431 268
243 212 165 153 132 118 117 96
60
EcoNI CCTnn'n_nnAGG
Cuts at: 131 131
Size: 7109
EcoO109I rG'GnC_Cy
Cuts at: 136 4248 136
Size: 4112 2997
EcoRI G'AATT_C
Cuts at: 4113 4113
Size: 7109
EcoRV GAT'ATC
Cuts at: 2514 3600 2514
Size: 1086 6023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 3088 5128 2683
Size: 405 2040 4664
Fragments arranged by size:
4664 2040 405
FspI TGC'GCA
Cuts at: 4890 4890
Size: 7109
HaeII r_GCGC'y
Cuts at: 4543 4791 5813 6792 4543
Size: 248 1022 979 4860
Fragments arranged by size:
4860 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 496 528 918 950 2086 2118 4009 4041 4951
Size: 32 390 32 1136 32 1891 32 910
Cuts at: 4951 4981 4983 5013 496
Size: 30 2 30 2592
Fragments arranged by size:
2592 1891 1136 910 390 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1233 1829 3069 4148 1233
Size: 596 1240 1079 4194
Fragments arranged by size:
4194 1240 1079 596
HindIII A'AGCT_T
Cuts at: 1971 3490 1971
Size: 1519 5590
KasI G'GCGC_C
Cuts at: 4539 4787 4539
Size: 248 6861
KpnI G_GTAC'C
Cuts at: 4129 4129
Size: 7109
MfeI C'AATT_G
Cuts at: 284 779 953 2021 284
Size: 495 174 1068 5372
Fragments arranged by size:
5372 1068 495 174
MluI A'CGCG_T
Cuts at: 6542 6542
Size: 7109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1708 2790 3414 5780 5964 1708
Size: 1082 624 2366 184 2853
Fragments arranged by size:
2853 2366 1082 624 184
MscI TGG'CCA
Cuts at: 3133 4870 3133
Size: 1737 5372
MslI CAynn'nnrTG
Cuts at: 1022 1873 5098 5225 1022
Size: 851 3225 127 2906
Fragments arranged by size:
3225 2906 851 127
MspA1I CmG'CkG
Cuts at: 1285 2382 2482 3462 4220 4534 4894 5907 6152
Size: 1097 100 980 758 314 360 1013 245
Cuts at: 6152 1285
Size: 2242
Fragments arranged by size:
2242 1097 1013 980 758 360 314 245
100
NaeI GCC'GGC
Cuts at: 5290 5290
Size: 7109
NarI GG'CG_CC
Cuts at: 4540 4788 4540
Size: 248 6861
NdeI CA'TA_TG
Cuts at: 227 227
Size: 7109
NgoMIV G'CCGG_C
Cuts at: 5288 5288
Size: 7109
NsiI A_TGCA'T
Cuts at: 2854 3245 3656 2854
Size: 391 411 6307
Fragments arranged by size:
6307 411 391
NspI r_CATG'y
Cuts at: 1021 1413 3243 3658 3916 4162 4662 5569 1021
Size: 392 1830 415 258 246 500 907 2561
Fragments arranged by size:
2561 1830 907 500 415 392 258 246
PciI A'CATG_T
Cuts at: 1409 3912 5565 1409
Size: 2503 1653 2953
Fragments arranged by size:
2953 2503 1653
PfoI T'CCnGG_A
Cuts at: 1110 1110
Size: 7109
PmeI GTTT'AAAC
Cuts at: 4108 4108
Size: 7109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1785 1817 6248 6280 1785
Size: 32 4431 32 2614
Fragments arranged by size:
4431 2614 32 32
PpuMI rG'GwC_Cy
Cuts at: 136 136
Size: 7109
PsiI TTA'TAA
Cuts at: 3050 3050
Size: 7109
PspXI vC'TCGA_Gb
Cuts at: 3287 3287
Size: 7109
PstI C_TGCA'G
Cuts at: 2222 2742 4156 4841 2222
Size: 520 1414 685 4490
Fragments arranged by size:
4490 1414 685 520
PvuI CG_AT'CG
Cuts at: 104 936 104
Size: 832 6277
PvuII CAG'CTG
Cuts at: 1285 2382 3462 4534 4894 1285
Size: 1097 1080 1072 360 3500
Fragments arranged by size:
3500 1097 1080 1072 360
RsrII CG'GwC_CG
Cuts at: 5304 5304
Size: 7109
SacI G_AGCT'C
Cuts at: 709 1164 3032 3640 4022 4036 4123 709
Size: 455 1868 608 382 14 87 3695
Fragments arranged by size:
3695 1868 608 455 382 87 14
SalI G'TCGA_C
Cuts at: 3067 4146 3067
Size: 1079 6030
SapI GCTCTTCn'nnn_
Cuts at: 5132 5342 5132
Size: 210 6899
SbfI CC_TGCA'GG
Cuts at: 4156 4156
Size: 7109
ScaI AGT'ACT
Cuts at: 6626 6626
Size: 7109
SexAI A'CCwGG_T
Cuts at: 138 2925 138
Size: 2787 4322
SfcI C'TryA_G
Cuts at: 33 440 1478 2218 2738 3088 4152 4837 5830
Size: 407 1038 740 520 350 1064 685 993
Cuts at: 5830 6021 33
Size: 191 1121
Fragments arranged by size:
1121 1064 1038 993 740 685 520 407
350 191
SfoI GGC'GCC
Cuts at: 4541 4789 4541
Size: 248 6861
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7109
SmaI CCC'GGG
Cuts at: 4131 4131
Size: 7109
SmlI C'TyrA_G
Cuts at: 2756 3287 3667 4264 5179 5671 5933 2756
Size: 531 380 597 915 492 262 3932
Fragments arranged by size:
3932 915 597 531 492 380 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7109
SphI G_CATG'C
Cuts at: 3658 4162 4662 3658
Size: 504 500 6105
Fragments arranged by size:
6105 504 500
SspI AAT'ATT
Cuts at: 798 1991 798
Size: 1193 5916
StuI AGG'CCT
Cuts at: 2821 2821
Size: 7109
StyI C'CwwG_G
Cuts at: 3020 3681 4243 4597 3020
Size: 661 562 354 5532
Fragments arranged by size:
5532 661 562 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 238 4692 4825 5284 5493 238
Size: 4454 133 459 209 1854
Fragments arranged by size:
4454 1854 459 209 133
TatI w'GTAC_w
Cuts at: 1152 1398 1904 3219 3278 3441 3732 3757 4047
Size: 246 506 1315 59 163 291 25 290
Cuts at: 4047 6624 1152
Size: 2577 1637
Fragments arranged by size:
2577 1637 1315 506 291 290 246 163
59 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 701 733 3107 3139 6626 6658 701
Size: 32 2374 32 3487 32 1152
Fragments arranged by size:
3487 2374 1152 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4906 4906
Size: 7109
XbaI T'CTAG_A
Cuts at: 790 2091 4140 790
Size: 1301 2049 3759
Fragments arranged by size:
3759 2049 1301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1282 4604 114
Size: 1168 3322 2619
Fragments arranged by size:
3322 2619 1168
XhoI C'TCGA_G
Cuts at: 3287 3287
Size: 7109
XmaI C'CCGG_G
Cuts at: 4129 4129
Size: 7109
XmnI GAAnn'nnTTC
Cuts at: 3350 4097 3350
Size: 747 6362
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsiWI
BssHII BstXI Bsu36I CspCI DraIII FseI FspAI HpaI
NcoI NheI NotI NruI PacI PasI PflMI PmlI
PshAI PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI ZraI