Restriction map for
pF1KA0777
(Circular) (Six-base) MAPSORT of: pF1KA0777.seq Check: 8375 from: 1 to: 6413
pF1KA0777 6413 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2075 2075
Size: 6413
AccI GT'mk_AC
Cuts at: 380 2777 3451 380
Size: 2397 674 3342
Fragments arranged by size:
3342 2397 674
Acc65I G'GTAC_C
Cuts at: 3429 3429
Size: 6413
AclI AA'CG_TT
Cuts at: 6253 6253
Size: 6413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 849 1477 4238 4670 5417 5685 760
Size: 89 628 2761 432 747 268 1488
Fragments arranged by size:
2761 1488 747 628 432 268 89
AfeI AGC'GCT
Cuts at: 2438 2438
Size: 6413
AflIII A'CryG_T
Cuts at: 455 621 1881 3765 4869 5846 455
Size: 166 1260 1884 1104 977 1022
Fragments arranged by size:
1884 1260 1104 1022 977 166
AgeI A'CCGG_T
Cuts at: 4801 4801
Size: 6413
AhdI GACnn_n'nnGTC
Cuts at: 1698 2616 1698
Size: 918 5495
AleI CACnn'nnGTG
Cuts at: 2968 2968
Size: 6413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 6413
AlwNI CAG_nnn'CTG
Cuts at: 237 2075 2576 3252 3718 5285 237
Size: 1838 501 676 466 1567 1365
Fragments arranged by size:
1838 1567 1365 676 501 466
ApaI G_GGCC'C
Cuts at: 338 338
Size: 6413
ApaLI G'TGCA_C
Cuts at: 430 2600 2663 5183 430
Size: 2170 63 2520 1660
Fragments arranged by size:
2520 2170 1660 63
ApoI r'AATT_y
Cuts at: 419 718 1642 1680 2238 3367 3417 5766 6022
Size: 299 924 38 558 1129 50 2349 256
Cuts at: 6022 419
Size: 810
Fragments arranged by size:
2349 1129 924 810 558 299 256 50
38
AseI AT'TA_AT
Cuts at: 20 20
Size: 6413
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6413
AvaI C'yCGr_G
Cuts at: 1142 1446 1613 1802 1874 2413 3433 1142
Size: 304 167 189 72 539 1020 4122
Fragments arranged by size:
4122 1020 539 304 189 167 72
BamHI G'GATC_C
Cuts at: 687 2017 3438 687
Size: 1330 1421 3662
Fragments arranged by size:
3662 1421 1330
BanI G'GyrC_C
Cuts at: 244 1608 1623 1836 2087 3429 3843 4091 4126
Size: 1364 15 213 251 1342 414 248 35
Cuts at: 4126 244
Size: 2531
Fragments arranged by size:
2531 1364 1342 414 251 248 213 35
15
BanII G_rGCy'C
Cuts at: 338 1079 1327 2162 2452 3184 3293 3427 4457
Size: 741 248 835 290 732 109 134 1030
Cuts at: 4457 338
Size: 2294
Fragments arranged by size:
2294 1030 835 741 732 290 248 134
109
BbeI G_GCGC'C
Cuts at: 3847 4095 3847
Size: 248 6165
BbsI GAAGACnn'nnnn_
Cuts at: 656 656
Size: 6413
BbvCI CC'TCA_GC
Cuts at: 2440 3119 2440
Size: 679 5734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 180 214 180
Size: 34 6379
BciVI GTATCCnnnnn_n'
Cuts at: 4311 5072 6413 4311
Size: 761 1341 4311
Fragments arranged by size:
4311 1341 761
BclI T'GATC_A
Cuts at: 1980 2796 3021 3933 1980
Size: 816 225 912 4460
Fragments arranged by size:
4460 912 816 225
BglI GCCn_nnn'nGGC
Cuts at: 1814 2166 1814
Size: 352 6061
BglII A'GATC_T
Cuts at: 3928 5965 3928
Size: 2037 4376
BlpI GC'TnA_GC
Cuts at: 3525 3525
Size: 6413
Bme1580I G_kGCm'C
Cuts at: 338 434 1613 1628 1841 2092 2604 2667 4038
Size: 96 1179 15 213 251 512 63 1371
Cuts at: 4038 4131 5187 338
Size: 93 1056 1564
Fragments arranged by size:
1564 1371 1179 1056 512 251 213 96
93 63 15
BmgBI CAC'GTC
Cuts at: 2556 2556
Size: 6413
BmrI ACTGGGnnnn_n'
Cuts at: 601 693 3625 3706 4041 6185 601
Size: 92 2932 81 335 2144 829
Fragments arranged by size:
2932 2144 829 335 92 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 973 1362 2439 4780 973
Size: 389 1077 2341 2606
Fragments arranged by size:
2606 2341 1077 389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 617 677 1077 1230 1605 1792 2672 3307
Size: 464 60 400 153 375 187 880 635
Cuts at: 3307 153
Size: 3259
Fragments arranged by size:
3259 880 635 464 400 375 187 153
60
Bpu10I CC'TnA_GC
Cuts at: 2440 3119 2440
Size: 679 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3198 3339 3589 4468 4960 5258 3198
Size: 141 250 879 492 298 4353
Fragments arranged by size:
4353 879 492 298 250 141
BsaI GGTCTCn'nnnn_
Cuts at: 6117 6117
Size: 6413
BsaAI yAC'GTr
Cuts at: 1884 2856 3766 1884
Size: 972 910 4531
Fragments arranged by size:
4531 972 910
BsaBI GATnn'nnATC
Cuts at: 6 3927 5964 6
Size: 3921 2037 455
Fragments arranged by size:
3921 2037 455
BsaHI Gr'CG_yC
Cuts at: 3844 4092 4794 6290 3844
Size: 248 702 1496 3967
Fragments arranged by size:
3967 1496 702 248
BsaWI w'CCGG_w
Cuts at: 349 2225 3602 4123 4801 5075 5222 5633 349
Size: 1876 1377 521 678 274 147 411 1129
Fragments arranged by size:
1876 1377 1129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1218 1248 2759 2789 2984 3014 1218
Size: 30 1511 30 195 30 4617
Fragments arranged by size:
4617 1511 195 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1354 1618 2170 2457 2460 1354
Size: 264 552 287 3 5307
Fragments arranged by size:
5307 552 287 264 3
BseYI C'CCAG_C
Cuts at: 153 221 2912 3838 4596 5173 5669 153
Size: 68 2691 926 758 577 496 897
Fragments arranged by size:
2691 926 897 758 577 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2362 2440 2362
Size: 78 6335
BsiEI CG_ry'CG
Cuts at: 104 384 3955 4001 5209 104
Size: 280 3571 46 1208 1308
Fragments arranged by size:
3571 1308 1208 280 46
BsiHKAI G_wGCw'C
Cuts at: 434 513 1597 1881 2604 2667 3184 3293 3427
Size: 79 1084 284 723 63 517 109 134
Cuts at: 3427 4205 4395 5187 434
Size: 778 190 792 1660
Fragments arranged by size:
1660 1084 792 778 723 517 284 190
134 109 79 63
BsmI GAATG_Cn'
Cuts at: 2534 2534
Size: 6413
BsmBI CGTCTCn'nnnn_
Cuts at: 1236 1236
Size: 6413
Bsp1286I G_dGCh'C
Cuts at: 338 434 513 1079 1327 1597 1613 1628 1841
Size: 96 79 566 248 270 16 15 213
Cuts at: 1841 1881 2092 2162 2452 2604 2667 3184 3293
Size: 40 211 70 290 152 63 517 109
Cuts at: 3293 3427 4038 4131 4205 4395 4457 5187 338
Size: 134 611 93 74 190 62 730 1564
Fragments arranged by size:
1564 730 611 566 517 290 270 248
213 211 190 152 134 109 96 93
79 74 70 63 62 40 16 15
BspEI T'CCGG_A
Cuts at: 349 2225 5633 349
Size: 1876 3408 1129
Fragments arranged by size:
3408 1876 1129
BspHI T'CATG_A
Cuts at: 3213 5589 3213
Size: 2376 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 475 1580 2075 3463 3677 3979 4360 475
Size: 1105 495 1388 214 302 381 2528
Fragments arranged by size:
2528 1388 1105 495 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1801 4705 4759 6243 6408 50
Size: 1751 2904 54 1484 165 55
Fragments arranged by size:
2904 1751 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4325 4325
Size: 6413
BsrFI r'CCGG_y
Cuts at: 383 1808 4411 4592 4801 383
Size: 1425 2603 181 209 1995
Fragments arranged by size:
2603 1995 1425 209 181
BsrGI T'GTAC_A
Cuts at: 491 1463 491
Size: 972 5441
BssHII G'CGCG_C
Cuts at: 1578 1578
Size: 6413
BssSI C'ACGA_G
Cuts at: 4684 5042 4684
Size: 358 6055
BstAPI GCAn_nnn'nTGC
Cuts at: 2890 3790 2890
Size: 900 5513
BstBI TT'CG_AA
Cuts at: 1646 4774 1646
Size: 3128 3285
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2593
Size: 6413
BstYI r'GATC_y
Cuts at: 1 687 2017 3438 3905 3928 4263 4509 5521
Size: 686 1330 1421 467 23 335 246 1012
Cuts at: 5521 5607 5619 5965 1
Size: 86 12 346 449
Fragments arranged by size:
1421 1330 1012 686 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2785 2785
Size: 6413
BtgI C'CryG_G
Cuts at: 338 904 1364 1598 2592 2823 338
Size: 566 460 234 994 231 3928
Fragments arranged by size:
3928 994 566 460 234 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1877 4363 4544 4707 1877
Size: 2486 181 163 3583
Fragments arranged by size:
3583 2486 181 163
BtsI GCAGTG_nn'
Cuts at: 899 1971 2473 2544 3656 899
Size: 1072 502 71 1112 3656
Fragments arranged by size:
3656 1112 1072 502 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 893 928 893
Size: 35 6378
DraI TTT'AAA
Cuts at: 3412 3412
Size: 6413
DraIII CAC_nnn'GTG
Cuts at: 429 429
Size: 6413
DrdI GACnn_nn'nnGTC
Cuts at: 1303 4119 4977 1303
Size: 2816 858 2739
Fragments arranged by size:
2816 2739 858
EaeI y'GGCC_r
Cuts at: 854 3998 4172 4563 4590 5935 854
Size: 3144 174 391 27 1345 1332
Fragments arranged by size:
3144 1345 1332 391 174 27
EagI C'GGCC_G
Cuts at: 3998 3998
Size: 6413
EarI CTCTTCn'nnn_
Cuts at: 281 286 874 1005 2673 4436 4646 281
Size: 5 588 131 1668 1763 210 2048
Fragments arranged by size:
2048 1763 1668 588 210 131 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2894 4621 4931 5077 6223 2894
Size: 1727 310 146 1146 3084
Fragments arranged by size:
3084 1727 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3182 3291 3425 3182
Size: 109 134 6170
Fragments arranged by size:
6170 134 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 617 677 760 849 1077 1230 1477 1605
Size: 464 60 83 89 228 153 247 128
Cuts at: 1605 1792 2672 3307 4238 4670 5417 5685 153
Size: 187 880 635 931 432 747 268 881
Fragments arranged by size:
931 881 880 747 635 464 432 268
247 228 187 153 128 89 83 60
EcoO109I rG'GnC_Cy
Cuts at: 1376 1494 1569 3350 3552 1376
Size: 118 75 1781 202 4237
Fragments arranged by size:
4237 1781 202 118 75
EcoRI G'AATT_C
Cuts at: 1642 3417 1642
Size: 1775 4638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4432 4432
Size: 6413
FspI TGC'GCA
Cuts at: 230 4194 230
Size: 3964 2449
HaeII r_GCGC'y
Cuts at: 2440 3847 4095 5117 6096 2440
Size: 1407 248 1022 979 2757
Fragments arranged by size:
2757 1407 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 287 319 586 618 1403 1435 1684
Size: 32 153 32 267 32 785 32 249
Cuts at: 1684 1716 1939 1971 2602 2634 2759 2791 2822
Size: 32 223 32 631 32 125 32 31
Cuts at: 2822 2854 3053 3085 4255 4285 4287 4317 102
Size: 32 199 32 1170 30 2 30 2198
Fragments arranged by size:
2198 1170 785 631 267 249 223 199
153 125 32 32 32 32 32 32
32 32 32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 381 3452 381
Size: 3071 3342
HindIII A'AGCT_T
Cuts at: 2007 2007
Size: 6413
KasI G'GCGC_C
Cuts at: 3843 4091 3843
Size: 248 6165
KpnI G_GTAC'C
Cuts at: 3433 3433
Size: 6413
MluI A'CGCG_T
Cuts at: 5846 5846
Size: 6413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 593 1292 1919 1946 5084 5268 593
Size: 699 627 27 3138 184 1738
Fragments arranged by size:
3138 1738 699 627 184 27
MscI TGG'CCA
Cuts at: 856 4174 856
Size: 3318 3095
MslI CAynn'nnrTG
Cuts at: 178 454 1133 2968 4402 4529 178
Size: 276 679 1835 1434 127 2062
Fragments arranged by size:
2062 1835 1434 679 276 127
MspA1I CmG'CkG
Cuts at: 2576 3524 3838 4198 5211 5456 2576
Size: 948 314 360 1013 245 3533
Fragments arranged by size:
3533 1013 948 360 314 245
NaeI GCC'GGC
Cuts at: 1810 4594 1810
Size: 2784 3629
NarI GG'CG_CC
Cuts at: 3844 4092 3844
Size: 248 6165
NcoI C'CATG_G
Cuts at: 1364 1364
Size: 6413
NgoMIV G'CCGG_C
Cuts at: 1808 4592 1808
Size: 2784 3629
NspI r_CATG'y
Cuts at: 229 459 625 1698 3466 3966 4873 229
Size: 230 166 1073 1768 500 907 1769
Fragments arranged by size:
1769 1768 1073 907 500 230 166
PasI CC'CwG_GG
Cuts at: 325 325
Size: 6413
PciI A'CATG_T
Cuts at: 455 621 4869 455
Size: 166 4248 1999
Fragments arranged by size:
4248 1999 166
PflMI CCAn_nnn'nTGG
Cuts at: 395 1370 2556 395
Size: 975 1186 4252
Fragments arranged by size:
4252 1186 975
PfoI T'CCnGG_A
Cuts at: 1249 1292 1388 1531 1249
Size: 43 96 143 6131
Fragments arranged by size:
6131 143 96 43
PmeI GTTT'AAAC
Cuts at: 3412 3412
Size: 6413
PmlI CAC'GTG
Cuts at: 1884 1884
Size: 6413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5552 5584 5552
Size: 32 6381
PpuMI rG'GwC_Cy
Cuts at: 1376 1494 1569 3350 1376
Size: 118 75 1781 4439
Fragments arranged by size:
4439 1781 118 75
PshAI GACnn'nnGTC
Cuts at: 1428 1428
Size: 6413
PspOMI G'GGCC_C
Cuts at: 334 334
Size: 6413
PstI C_TGCA'G
Cuts at: 894 1544 1966 3460 4145 894
Size: 650 422 1494 685 3162
Fragments arranged by size:
3162 1494 685 650 422
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6413
PvuII CAG'CTG
Cuts at: 2576 3838 4198 2576
Size: 1262 360 4791
Fragments arranged by size:
4791 1262 360
RsrII CG'GwC_CG
Cuts at: 4608 4608
Size: 6413
SacI G_AGCT'C
Cuts at: 3184 3293 3427 3184
Size: 109 134 6170
Fragments arranged by size:
6170 134 109
SalI G'TCGA_C
Cuts at: 379 3450 379
Size: 3071 3342
SanDI GG'GwC_CC
Cuts at: 1376 1376
Size: 6413
SapI GCTCTTCn'nnn_
Cuts at: 874 1005 4436 4646 874
Size: 131 3431 210 2641
Fragments arranged by size:
3431 2641 210 131
SbfI CC_TGCA'GG
Cuts at: 3460 3460
Size: 6413
ScaI AGT'ACT
Cuts at: 5930 5930
Size: 6413
SfcI C'TryA_G
Cuts at: 33 504 543 812 890 1110 1540 1962 2936
Size: 471 39 269 78 220 430 422 974
Cuts at: 2936 3456 4141 5134 5325 33
Size: 520 685 993 191 1121
Fragments arranged by size:
1121 993 974 685 520 471 430 422
269 220 191 78 39
SfoI GGC'GCC
Cuts at: 3845 4093 3845
Size: 248 6165
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6413
SmaI CCC'GGG
Cuts at: 1448 1615 3435 1448
Size: 167 1820 4426
Fragments arranged by size:
4426 1820 167
SmlI C'TyrA_G
Cuts at: 3177 3354 3568 4483 4975 5237 3177
Size: 177 214 915 492 262 4353
Fragments arranged by size:
4353 915 492 262 214 177
SnaBI TAC'GTA
Cuts at: 2856 2856
Size: 6413
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6413
SphI G_CATG'C
Cuts at: 229 3466 3966 229
Size: 3237 500 2676
Fragments arranged by size:
3237 2676 500
SspI AAT'ATT
Cuts at: 3222 3222
Size: 6413
StyI C'CwwG_G
Cuts at: 389 733 1349 1364 2668 3547 3901 389
Size: 344 616 15 1304 879 354 2901
Fragments arranged by size:
2901 1304 879 616 354 344 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 650 713 2486 3996 4129 4588 4797 650
Size: 63 1773 1510 133 459 209 2266
Fragments arranged by size:
2266 1773 1510 459 209 133 63
TatI w'GTAC_w
Cuts at: 491 1463 5928 491
Size: 972 4465 976
Fragments arranged by size:
4465 976 972
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 620 652 5930 5962 178
Size: 32 410 32 5278 32 629
Fragments arranged by size:
5278 629 410 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4210 4210
Size: 6413
XbaI T'CTAG_A
Cuts at: 3444 3444
Size: 6413
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 3908 2590
Size: 1318 5095
XmaI C'CCGG_G
Cuts at: 1446 1613 3433 1446
Size: 167 1820 4426
Fragments arranged by size:
4426 1820 167
XmnI GAAnn'nnTTC
Cuts at: 3221 3242 3221
Size: 21 6392
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BstEII BstZ17I ClaI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI