Restriction map for
pF1KA0774
(Circular) (Six-base) MAPSORT of: pF1KA0774.seq Check: 6903 from: 1 to: 7250
pF1KA0774 7250 bp
With 182 enzymes: SgfI *
March 20, 2014 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 930 1381 2182 3525 930
Size: 451 801 1343 4655
Fragments arranged by size:
4655 1343 801 451
AccI GT'mk_AC
Cuts at: 4288 4288
Size: 7250
Acc65I G'GTAC_C
Cuts at: 4266 4266
Size: 7250
AclI AA'CG_TT
Cuts at: 7090 7090
Size: 7250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 2224 2247 2498 2564 2836 3160 3214 3883
Size: 1860 23 251 66 272 324 54 669
Cuts at: 3883 5075 5507 6254 6522 364
Size: 1192 432 747 268 1092
Fragments arranged by size:
1860 1192 1092 747 669 432 324 272
268 251 66 54 23
AflII C'TTAA_G
Cuts at: 225 2434 225
Size: 2209 5041
AflIII A'CryG_T
Cuts at: 295 930 1883 2155 4602 5706 6683 295
Size: 635 953 272 2447 1104 977 862
Fragments arranged by size:
2447 1104 977 953 862 635 272
AgeI A'CCGG_T
Cuts at: 5638 5638
Size: 7250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3077 3077
Size: 7250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3364 3396 3364
Size: 32 7218
AlwNI CAG_nnn'CTG
Cuts at: 982 2100 3435 3507 3753 4555 6122 982
Size: 1118 1335 72 246 802 1567 2110
Fragments arranged by size:
2110 1567 1335 1118 802 246 72
ApaI G_GGCC'C
Cuts at: 4211 4211
Size: 7250
ApaLI G'TGCA_C
Cuts at: 1868 2750 6020 1868
Size: 882 3270 3098
Fragments arranged by size:
3270 3098 882
ApoI r'AATT_y
Cuts at: 327 367 596 990 1376 2230 3687 4254 6603
Size: 40 229 394 386 854 1457 567 2349
Cuts at: 6603 6859 327
Size: 256 718
Fragments arranged by size:
2349 1457 854 718 567 394 386 256
229 40
AscI GG'CGCG_CC
Cuts at: 3475 3475
Size: 7250
AseI AT'TA_AT
Cuts at: 20 20
Size: 7250
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7250
AvaI C'yCGr_G
Cuts at: 743 2298 4203 4270 743
Size: 1555 1905 67 3723
Fragments arranged by size:
3723 1905 1555 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2649 2682 2649
Size: 33 7217
BamHI G'GATC_C
Cuts at: 1176 1925 4275 1176
Size: 749 2350 4151
Fragments arranged by size:
4151 2350 749
BanI G'GyrC_C
Cuts at: 1584 4266 4680 4928 4963 1584
Size: 2682 414 248 35 3871
Fragments arranged by size:
3871 2682 414 248 35
BanII G_rGCy'C
Cuts at: 1336 1595 2710 3428 3768 4211 4264 5294 1336
Size: 259 1115 718 340 443 53 1030 3292
Fragments arranged by size:
3292 1115 1030 718 443 340 259 53
BbeI G_GCGC'C
Cuts at: 4684 4932 4684
Size: 248 7002
BbsI GAAGACnn'nnnn_
Cuts at: 796 1932 2253 2504 3854 3998 796
Size: 1136 321 251 1350 144 4048
Fragments arranged by size:
4048 1350 1136 321 251 144
BbvCI CC'TCA_GC
Cuts at: 1704 1704
Size: 7250
BciVI GTATCCnnnnn_n'
Cuts at: 2650 3037 5148 5909 7250 2650
Size: 387 2111 761 1341 2650
Fragments arranged by size:
2650 2111 1341 761 387
BclI T'GATC_A
Cuts at: 4770 4770
Size: 7250
BglI GCCn_nnn'nGGC
Cuts at: 2683 3112 2683
Size: 429 6821
BglII A'GATC_T
Cuts at: 3543 4765 6802 3543
Size: 1222 2037 3991
Fragments arranged by size:
3991 2037 1222
BlpI GC'TnA_GC
Cuts at: 3116 3508 4362 3116
Size: 392 854 6004
Fragments arranged by size:
6004 854 392
Bme1580I G_kGCm'C
Cuts at: 930 1872 2754 3267 4211 4875 4968 6024 930
Size: 942 882 513 944 664 93 1056 2156
Fragments arranged by size:
2156 1056 944 942 882 664 513 93
BmgBI CAC'GTC
Cuts at: 1886 2665 1886
Size: 779 6471
BmrI ACTGGGnnnn_n'
Cuts at: 142 1784 1913 2730 4151 4462 4543 4878 7022
Size: 1642 129 817 1421 311 81 335 2144
Cuts at: 7022 142
Size: 370
Fragments arranged by size:
2144 1642 1421 817 370 335 311 129
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 288 725 3767 5617 288
Size: 437 3042 1850 1921
Fragments arranged by size:
3042 1921 1850 437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2694 2712 2855 2865 3391 3412 3457 3832 4045
Size: 18 143 10 526 21 45 375 213
Cuts at: 4045 2694
Size: 5899
Fragments arranged by size:
5899 526 375 213 143 45 21 18
10
Bpu10I CC'TnA_GC
Cuts at: 1589 1704 1589
Size: 115 7135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3807 3940 4426 5305 5797 6095 3807
Size: 133 486 879 492 298 4962
Fragments arranged by size:
4962 879 492 486 298 133
BsaI GGTCTCn'nnnn_
Cuts at: 904 2647 6954 904
Size: 1743 4307 1200
Fragments arranged by size:
4307 1743 1200
BsaAI yAC'GTr
Cuts at: 4603 4603
Size: 7250
BsaBI GATnn'nnATC
Cuts at: 6 4764 6801 6
Size: 4758 2037 455
Fragments arranged by size:
4758 2037 455
BsaHI Gr'CG_yC
Cuts at: 2114 4681 4929 5631 7127 2114
Size: 2567 248 702 1496 2237
Fragments arranged by size:
2567 2237 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2269 2702 3515 4439 4960 5638 5912 6059 6470
Size: 433 813 924 521 678 274 147 411
Cuts at: 6470 2269
Size: 3049
Fragments arranged by size:
3049 924 813 678 521 433 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3364 3394 4180 4210 3364
Size: 30 786 30 6404
Fragments arranged by size:
6404 786 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 669 1298 1513 2023 2369 2709 3379 3454
Size: 405 629 215 510 346 340 670 75
Cuts at: 3454 3502 3817 4188 4191 4203 264
Size: 48 315 371 3 12 3311
Fragments arranged by size:
3311 670 629 510 405 371 346 340
315 215 75 48 12 3
BseYI C'CCAG_C
Cuts at: 2441 3171 3402 4675 5433 6010 6506 2441
Size: 730 231 1273 758 577 496 3185
Fragments arranged by size:
3185 1273 758 730 577 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 966 1409 966
Size: 443 6807
BsiEI CG_ry'CG
Cuts at: 104 2859 4792 4838 6046 104
Size: 2755 1933 46 1208 1308
Fragments arranged by size:
2755 1933 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 473 901 956 1872 2710 2754 3428 3494 4264
Size: 428 55 916 838 44 674 66 770
Cuts at: 4264 5042 5232 6024 473
Size: 778 190 792 1699
Fragments arranged by size:
1699 916 838 792 778 770 674 428
190 66 55 44
BsmI GAATG_Cn'
Cuts at: 249 660 1686 249
Size: 411 1026 5813
Fragments arranged by size:
5813 1026 411
BsmBI CGTCTCn'nnnn_
Cuts at: 2671 2721 2671
Size: 50 7200
Bsp1286I G_dGCh'C
Cuts at: 473 901 930 956 1336 1595 1872 2710 2754
Size: 428 29 26 380 259 277 838 44
Cuts at: 2754 3267 3428 3494 3768 4211 4264 4875 4968
Size: 513 161 66 274 443 53 611 93
Cuts at: 4968 5042 5232 5294 6024 473
Size: 74 190 62 730 1699
Fragments arranged by size:
1699 838 730 611 513 443 428 380
277 274 259 190 161 93 74 66
62 53 44 29 26
BspEI T'CCGG_A
Cuts at: 2269 2702 6470 2269
Size: 433 3768 3049
Fragments arranged by size:
3768 3049 433
BspHI T'CATG_A
Cuts at: 279 3657 6426 279
Size: 3378 2769 1103
Fragments arranged by size:
3378 2769 1103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 930 1381 2182 2252 2994 3525 3858 4300 4514
Size: 451 801 70 742 531 333 442 214
Cuts at: 4514 4816 5197 930
Size: 302 381 2983
Fragments arranged by size:
2983 801 742 531 451 442 381 333
302 214 70
BsrBI CCG'CTC
Cuts at: 50 1616 3120 3147 3444 5542 5596 7080 7245
Size: 1566 1504 27 297 2098 54 1484 165
Cuts at: 7245 50
Size: 55
Fragments arranged by size:
2098 1566 1504 1484 297 165 55 54
27
BsrDI GCAATG_nn'
Cuts at: 793 2465 3709 5162 793
Size: 1672 1244 1453 2881
Fragments arranged by size:
2881 1672 1453 1244
BsrFI r'CCGG_y
Cuts at: 2768 2854 5248 5429 5638 2768
Size: 86 2394 181 209 4380
Fragments arranged by size:
4380 2394 209 181 86
BssHII G'CGCG_C
Cuts at: 3475 3475
Size: 7250
BssSI C'ACGA_G
Cuts at: 3898 5521 5879 3898
Size: 1623 358 5269
Fragments arranged by size:
5269 1623 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3129 4627 3129
Size: 1498 5752
BstBI TT'CG_AA
Cuts at: 3776 5611 3776
Size: 1835 5415
BstEII G'GTnAC_C
Cuts at: 1641 2197 3496 1641
Size: 556 1299 5395
Fragments arranged by size:
5395 1299 556
BstXI CCAn_nnnn'nTGG
Cuts at: 1249 3171 1249
Size: 1922 5328
BstYI r'GATC_y
Cuts at: 1 1068 1176 1925 3543 3965 4275 4742 4765
Size: 1067 108 749 1618 422 310 467 23
Cuts at: 4765 5100 5346 6358 6444 6456 6802 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1618 1067 1012 749 467 449 422 346
335 310 246 108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1344 1344
Size: 7250
BtgI C'CryG_G
Cuts at: 107 3227 4191 107
Size: 3120 964 3166
Fragments arranged by size:
3166 3120 964
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5200 5381 5544 5200
Size: 181 163 6906
Fragments arranged by size:
6906 181 163
BtsI GCAGTG_nn'
Cuts at: 954 1524 4493 954
Size: 570 2969 3711
Fragments arranged by size:
3711 2969 570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1635 1670 1635
Size: 35 7215
DraI TTT'AAA
Cuts at: 2591 4249 2591
Size: 1658 5592
DraIII CAC_nnn'GTG
Cuts at: 477 1387 1435 477
Size: 910 48 6292
Fragments arranged by size:
6292 910 48
DrdI GACnn_nn'nnGTC
Cuts at: 808 3634 4956 5814 808
Size: 2826 1322 858 2244
Fragments arranged by size:
2826 2244 1322 858
EaeI y'GGCC_r
Cuts at: 1218 1611 2770 2856 3230 4220 4835 5009 5400
Size: 393 1159 86 374 990 615 174 391
Cuts at: 5400 5427 6772 1218
Size: 27 1345 1696
Fragments arranged by size:
1696 1345 1159 990 615 393 391 374
174 86 27
EagI C'GGCC_G
Cuts at: 2856 4835 2856
Size: 1979 5271
EarI CTCTTCn'nnn_
Cuts at: 461 1142 1254 1268 1273 2174 2360 3190 3391
Size: 681 112 14 5 901 186 830 201
Cuts at: 3391 3773 3850 4090 4112 5273 5483 461
Size: 382 77 240 22 1161 210 2228
Fragments arranged by size:
2228 1161 901 830 681 382 240 210
201 186 112 77 22 14 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 957 3074 3481 5458 5768 5914 7060 957
Size: 2117 407 1977 310 146 1146 1147
Fragments arranged by size:
2117 1977 1147 1146 407 310 146
EcoICRI GAG'CTC
Cuts at: 2708 3426 4262 2708
Size: 718 836 5696
Fragments arranged by size:
5696 836 718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 2224 2247 2498 2564 2694 2712 2836 2855
Size: 1860 23 251 66 130 18 124 19
Cuts at: 2855 2865 3160 3214 3391 3412 3457 3832 3883
Size: 10 295 54 177 21 45 375 51
Cuts at: 3883 4045 5075 5507 6254 6522 364
Size: 162 1030 432 747 268 1092
Fragments arranged by size:
1860 1092 1030 747 432 375 295 268
251 177 162 130 124 66 54 51
45 23 21 19 18 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2079 2104 3484 2079
Size: 25 1380 5845
Fragments arranged by size:
5845 1380 25
EcoO109I rG'GnC_Cy
Cuts at: 1835 2097 2539 4146 4207 4208 4389 1835
Size: 262 442 1607 61 1 181 4696
Fragments arranged by size:
4696 1607 442 262 181 61 1
EcoRI G'AATT_C
Cuts at: 4254 4254
Size: 7250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1956 4141 5269 1956
Size: 2185 1128 3937
Fragments arranged by size:
3937 2185 1128
FseI GG_CCGG'CC
Cuts at: 2772 2858 2772
Size: 86 7164
FspI TGC'GCA
Cuts at: 3182 5031 3182
Size: 1849 5401
HaeII r_GCGC'y
Cuts at: 2525 2920 4684 4932 5954 6933 2525
Size: 395 1764 248 1022 979 2842
Fragments arranged by size:
2842 1764 1022 979 395 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 846 858 878 943 975 1141 1173 1819
Size: 20 12 20 65 32 166 32 646
Cuts at: 1819 1851 2487 2519 3639 3671 5092 5122 5124
Size: 32 636 32 1120 32 1421 30 2
Cuts at: 5124 5154 826
Size: 30 2922
Fragments arranged by size:
2922 1421 1120 646 636 166 65 32
32 32 32 32 30 30 20 20
12 2
HincII GTy'rAC
Cuts at: 134 1290 3523 4289 134
Size: 1156 2233 766 3095
Fragments arranged by size:
3095 2233 1156 766
HindIII A'AGCT_T
Cuts at: 2597 3028 2597
Size: 431 6819
KasI G'GCGC_C
Cuts at: 4680 4928 4680
Size: 248 7002
KpnI G_GTAC'C
Cuts at: 4270 4270
Size: 7250
MluI A'CGCG_T
Cuts at: 6683 6683
Size: 7250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 242 520 1545 2431 4002 4022 5921 6105 242
Size: 278 1025 886 1571 20 1899 184 1387
Fragments arranged by size:
1899 1571 1387 1025 886 278 184 20
MscI TGG'CCA
Cuts at: 1220 3232 5011 1220
Size: 2012 1779 3459
Fragments arranged by size:
3459 2012 1779
MslI CAynn'nnrTG
Cuts at: 2035 5239 5366 2035
Size: 3204 127 3919
Fragments arranged by size:
3919 3204 127
MspA1I CmG'CkG
Cuts at: 2941 3126 3171 3453 3504 3810 4038 4193 4361
Size: 185 45 282 51 306 228 155 168
Cuts at: 4361 4675 5035 6048 6293 2941
Size: 314 360 1013 245 3898
Fragments arranged by size:
3898 1013 360 314 306 282 245 228
185 168 155 51 45
NaeI GCC'GGC
Cuts at: 2770 2856 5431 2770
Size: 86 2575 4589
Fragments arranged by size:
4589 2575 86
NarI GG'CG_CC
Cuts at: 4681 4929 4681
Size: 248 7002
NcoI C'CATG_G
Cuts at: 107 107
Size: 7250
NdeI CA'TA_TG
Cuts at: 2142 2142
Size: 7250
NgoMIV G'CCGG_C
Cuts at: 2768 2854 5429 2768
Size: 86 2575 4589
Fragments arranged by size:
4589 2575 86
NspI r_CATG'y
Cuts at: 299 610 934 2056 2159 4303 4803 5710 299
Size: 311 324 1122 103 2144 500 907 1839
Fragments arranged by size:
2144 1839 1122 907 500 324 311 103
PasI CC'CwG_GG
Cuts at: 1294 2100 1294
Size: 806 6444
PciI A'CATG_T
Cuts at: 295 930 2155 5706 295
Size: 635 1225 3551 1839
Fragments arranged by size:
3551 1839 1225 635
PflMI CCAn_nnn'nTGG
Cuts at: 921 2100 3435 921
Size: 1179 1335 4736
Fragments arranged by size:
4736 1335 1179
PfoI T'CCnGG_A
Cuts at: 2652 3428 4061 2652
Size: 776 633 5841
Fragments arranged by size:
5841 776 633
PmeI GTTT'AAAC
Cuts at: 4249 4249
Size: 7250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3364 3396 6389 6421 3364
Size: 32 2993 32 4193
Fragments arranged by size:
4193 2993 32 32
PpuMI rG'GwC_Cy
Cuts at: 1835 2539 4146 1835
Size: 704 1607 4939
Fragments arranged by size:
4939 1607 704
PshAI GACnn'nnGTC
Cuts at: 4038 4038
Size: 7250
PspOMI G'GGCC_C
Cuts at: 4207 4207
Size: 7250
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1756 1788 1756
Size: 32 7218
PstI C_TGCA'G
Cuts at: 503 696 941 2249 3452 3485 3617 4297 4982
Size: 193 245 1308 1203 33 132 680 685
Cuts at: 4982 503
Size: 2771
Fragments arranged by size:
2771 1308 1203 685 680 245 193 132
33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7250
PvuII CAG'CTG
Cuts at: 3126 3171 3453 3504 3810 4038 4675 5035 3126
Size: 45 282 51 306 228 637 360 5341
Fragments arranged by size:
5341 637 360 306 282 228 51 45
RsrII CG'GwC_CG
Cuts at: 5445 5445
Size: 7250
SacI G_AGCT'C
Cuts at: 2710 3428 4264 2710
Size: 718 836 5696
Fragments arranged by size:
5696 836 718
SacII CC_GC'GG
Cuts at: 4194 4194
Size: 7250
SalI G'TCGA_C
Cuts at: 4287 4287
Size: 7250
SanDI GG'GwC_CC
Cuts at: 1835 4146 1835
Size: 2311 4939
SapI GCTCTTCn'nnn_
Cuts at: 461 4112 5273 5483 461
Size: 3651 1161 210 2228
Fragments arranged by size:
3651 2228 1161 210
SbfI CC_TGCA'GG
Cuts at: 3485 3617 4297 3485
Size: 132 680 6438
Fragments arranged by size:
6438 680 132
ScaI AGT'ACT
Cuts at: 6767 6767
Size: 7250
SexAI A'CCwGG_T
Cuts at: 1431 3719 1431
Size: 2288 4962
SfcI C'TryA_G
Cuts at: 33 499 686 692 937 2245 2565 3448 3481
Size: 466 187 6 245 1308 320 883 33
Cuts at: 3481 3613 4293 4978 5971 6162 33
Size: 132 680 685 993 191 1121
Fragments arranged by size:
1308 1121 993 883 685 680 466 320
245 191 187 132 33 6
SfoI GGC'GCC
Cuts at: 4682 4930 4682
Size: 248 7002
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7250
SmaI CCC'GGG
Cuts at: 745 2300 4272 745
Size: 1555 1972 3723
Fragments arranged by size:
3723 1972 1555
SmlI C'TyrA_G
Cuts at: 225 2434 3786 3919 4405 5320 5812 6074 225
Size: 2209 1352 133 486 915 492 262 1401
Fragments arranged by size:
2209 1401 1352 915 492 486 262 133
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7250
SphI G_CATG'C
Cuts at: 4303 4803 4303
Size: 500 6750
SspI AAT'ATT
Cuts at: 1731 1731
Size: 7250
StuI AGG'CCT
Cuts at: 3784 3784
Size: 7250
StyI C'CwwG_G
Cuts at: 107 614 1163 1248 1484 1841 1952 2254 2846
Size: 507 549 85 236 357 111 302 592
Cuts at: 2846 4384 4738 107
Size: 1538 354 2619
Fragments arranged by size:
2619 1538 592 549 507 357 354 302
236 111 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1393 1763 1803 2408 2443 2546 2749 2859 4254
Size: 370 40 605 35 103 203 110 1395
Cuts at: 4254 4833 4966 5425 5634 1393
Size: 579 133 459 209 3009
Fragments arranged by size:
3009 1395 605 579 459 370 209 203
133 110 103 40 35
TatI w'GTAC_w
Cuts at: 933 6765 933
Size: 5832 1418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 842 874 2264 2296 6767 6799 842
Size: 32 1390 32 4471 32 1293
Fragments arranged by size:
4471 1390 1293 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 527 5047 527
Size: 4520 2730
XbaI T'CTAG_A
Cuts at: 567 1674 4281 567
Size: 1107 2607 3536
Fragments arranged by size:
3536 2607 1107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 621 4745 621
Size: 4124 3126
XmaI C'CCGG_G
Cuts at: 743 2298 4270 743
Size: 1555 1972 3723
Fragments arranged by size:
3723 1972 1555
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AvrII BcgI BfrBI BmtI
BsiWI BsrGI BstZ17I ClaI EcoRV FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PmlI PsiI PspXI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI