Restriction map for
pF1KA0773
(Circular) (Six-base) MAPSORT of: pF1KA0773.seq Check: 8931 from: 1 to: 4193
pF1KA0773 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 1136 1136
Size: 4193
AccI GT'mk_AC
Cuts at: 1105 1231 1105
Size: 126 4067
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 1187 4033 1187
Size: 2846 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 899 2018 2450 2803 3465 190
Size: 709 1119 432 353 662 918
Fragments arranged by size:
1119 918 709 662 432 353
AflII C'TTAA_G
Cuts at: 319 319
Size: 4193
AflIII A'CryG_T
Cuts at: 761 1545 3345 3626 761
Size: 784 1800 281 1328
Fragments arranged by size:
1800 1328 784 281
AhdI GACnn_n'nnGTC
Cuts at: 1071 1071
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 702 1498 2936 702
Size: 796 1438 1959
Fragments arranged by size:
1959 1438 796
ApaI G_GGCC'C
Cuts at: 354 1006 354
Size: 652 3541
ApaLI G'TGCA_C
Cuts at: 3031 3031
Size: 4193
ApoI r'AATT_y
Cuts at: 1197 3546 3802 1197
Size: 2349 256 1588
Fragments arranged by size:
2349 1588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 1213 1213
Size: 4193
BamHI G'GATC_C
Cuts at: 844 1218 844
Size: 374 3819
BanI G'GyrC_C
Cuts at: 1032 1209 1623 1871 1906 1032
Size: 177 414 248 35 3319
Fragments arranged by size:
3319 414 248 177 35
BanII G_rGCy'C
Cuts at: 354 972 1006 1207 2237 354
Size: 618 34 201 1030 2310
Fragments arranged by size:
2310 1030 618 201 34
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BbsI GAAGACnn'nnnn_
Cuts at: 475 614 1097 475
Size: 139 483 3571
Fragments arranged by size:
3571 483 139
BbvCI CC'TCA_GC
Cuts at: 538 1010 538
Size: 472 3721
BciVI GTATCCnnnnn_n'
Cuts at: 510 913 2091 3147 4193 510
Size: 403 1178 1056 1046 510
Fragments arranged by size:
1178 1056 1046 510 403
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BglI GCCn_nnn'nGGC
Cuts at: 835 835
Size: 4193
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 354 1006 1818 1911 3035 354
Size: 652 812 93 1124 1512
Fragments arranged by size:
1512 1124 812 652 93
BmrI ACTGGGnnnn_n'
Cuts at: 975 1405 1486 1821 3965 975
Size: 430 81 335 2144 1203
Fragments arranged by size:
2144 1203 430 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1090 952
Size: 138 4055
Bpu10I CC'TnA_GC
Cuts at: 538 1010 538
Size: 472 3721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1369 2248 2962 3260 713
Size: 656 879 714 298 1646
Fragments arranged by size:
1646 879 714 656 298
BsaI GGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 4193
BsaAI yAC'GTr
Cuts at: 762 1546 762
Size: 784 3409
BsaBI GATnn'nnATC
Cuts at: 6 179 1707 3744 6
Size: 173 1528 2037 455
Fragments arranged by size:
2037 1528 455 173
BsaHI Gr'CG_yC
Cuts at: 575 1133 1624 1872 2574 4070 575
Size: 558 491 248 702 1496 698
Fragments arranged by size:
1496 702 698 558 491 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2992 3139 3413 1382
Size: 521 678 411 147 274 2162
Fragments arranged by size:
2162 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1027 1030 1054 1180 1027
Size: 3 24 126 4040
Fragments arranged by size:
4040 126 24 3
BseYI C'CCAG_C
Cuts at: 461 880 948 1618 2376 3041 3449 461
Size: 419 68 670 758 665 408 1205
Fragments arranged by size:
1205 758 670 665 419 408 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1171 196
Size: 975 3218
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 3011 104
Size: 1631 46 1230 1286
Fragments arranged by size:
1631 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 3035 1207
Size: 778 190 860 2365
Fragments arranged by size:
2365 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 354 972 1006 1207 1818 1911 1985 2175 2237
Size: 618 34 201 611 93 74 190 62
Cuts at: 2237 3035 354
Size: 798 1512
Fragments arranged by size:
1512 798 618 611 201 190 93 74
62 34
BspHI T'CATG_A
Cuts at: 2625 2625
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1243 1457 1759 2140 1243
Size: 214 302 381 3296
Fragments arranged by size:
3296 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1111 2485 2539 4023 4188 50
Size: 1061 1374 54 1484 165 55
Fragments arranged by size:
1484 1374 1061 165 55 54
BsrDI GCAATG_nn'
Cuts at: 824 2105 824
Size: 1281 2912
BsrFI r'CCGG_y
Cuts at: 2191 2372 2191
Size: 181 4012
BssSI C'ACGA_G
Cuts at: 1085 2464 3172 1085
Size: 1379 708 2106
Fragments arranged by size:
2106 1379 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 420 612 420
Size: 192 4001
BstYI r'GATC_y
Cuts at: 1 377 844 1218 1685 1708 2043 2289 2595
Size: 376 467 374 467 23 335 246 306
Cuts at: 2595 2607 2693 3745 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 467 449 376 374 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1037 1143 1037
Size: 106 4087
BtgI C'CryG_G
Cuts at: 107 287 419 626 737 107
Size: 180 132 207 111 3563
Fragments arranged by size:
3563 207 180 132 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 557 2143 2324 2487 557
Size: 1586 181 163 2263
Fragments arranged by size:
2263 1586 181 163
BtsI GCAGTG_nn'
Cuts at: 158 208 992 1436 158
Size: 50 784 444 2915
Fragments arranged by size:
2915 784 444 50
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 3243 1899
Size: 1344 2849
EaeI y'GGCC_r
Cuts at: 232 356 836 1778 1952 2343 2370 3715 232
Size: 124 480 942 174 391 27 1345 710
Fragments arranged by size:
1345 942 710 480 391 174 124 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 1015 1040 2216 2426 1015
Size: 25 1176 210 2782
Fragments arranged by size:
2782 1176 210 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 3143 3289 4003 2401
Size: 742 146 714 2591
Fragments arranged by size:
2591 742 714 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 899 952 1090 2018 2450 2803 3465 190
Size: 709 53 138 928 432 353 662 918
Fragments arranged by size:
928 918 709 662 432 353 138 53
EcoO109I rG'GnC_Cy
Cuts at: 165 350 351 898 1002 1003 1065 1332 165
Size: 185 1 547 104 1 62 267 3026
Fragments arranged by size:
3026 547 267 185 104 62 1 1
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 4193
FspI TGC'GCA
Cuts at: 511 1974 511
Size: 1463 2730
HaeII r_GCGC'y
Cuts at: 1627 1875 3105 3876 1627
Size: 248 1230 771 1944
Fragments arranged by size:
1944 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 514 546 696 728 1057 1089 1123 1155 2035
Size: 32 150 32 329 32 34 32 880
Cuts at: 2035 2065 2067 2097 514
Size: 30 2 30 2610
Fragments arranged by size:
2610 880 329 150 34 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1232 1232
Size: 4193
HindIII A'AGCT_T
Cuts at: 316 316
Size: 4193
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 2952 3136 557
Size: 2395 184 1614
Fragments arranged by size:
2395 1614 184
MscI TGG'CCA
Cuts at: 358 1954 358
Size: 1596 2597
MslI CAynn'nnrTG
Cuts at: 418 497 610 646 2182 2309 418
Size: 79 113 36 1536 127 2302
Fragments arranged by size:
2302 1536 127 113 79 36
MspA1I CmG'CkG
Cuts at: 478 1304 1618 1978 2762 3007 478
Size: 826 314 360 784 245 1664
Fragments arranged by size:
1664 826 784 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NcoI C'CATG_G
Cuts at: 107 287 419 626 737 107
Size: 180 132 207 111 3563
Fragments arranged by size:
3563 207 180 132 111
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NspI r_CATG'y
Cuts at: 1246 1746 3349 1246
Size: 500 1603 2090
Fragments arranged by size:
2090 1603 500
PasI CC'CwG_GG
Cuts at: 768 768
Size: 4193
PciI A'CATG_T
Cuts at: 3345 3345
Size: 4193
PfoI T'CCnGG_A
Cuts at: 124 124
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 898 1065 898
Size: 167 4026
PspOMI G'GGCC_C
Cuts at: 350 1002 350
Size: 652 3541
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 478 1618 1978 478
Size: 1140 360 2693
Fragments arranged by size:
2693 1140 360
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SfcI C'TryA_G
Cuts at: 33 681 1236 1921 2889 3080 33
Size: 648 555 685 968 191 1146
Fragments arranged by size:
1146 968 685 648 555 191
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 319 728 1348 2263 2977 3239 319
Size: 409 620 915 714 262 1273
Fragments arranged by size:
1273 915 714 620 409 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 107 287 419 626 737 1327 1681 107
Size: 180 132 207 111 590 354 2619
Fragments arranged by size:
2619 590 354 207 180 132 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 673 750 1776 1909 2368 2577 673
Size: 77 1026 133 459 209 2289
Fragments arranged by size:
2289 1026 459 209 133 77
TatI w'GTAC_w
Cuts at: 776 3708 776
Size: 2932 1261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
ZraI GAC'GTC
Cuts at: 1134 1134
Size: 4193
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI