Restriction map for
pF1KA0765
(Circular) (Six-base) MAPSORT of: pF1KA0765.seq Check: 6884 from: 1 to: 5909
pF1KA0765 5909 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2947 2947
Size: 5909
Acc65I G'GTAC_C
Cuts at: 2925 2925
Size: 5909
AclI AA'CG_TT
Cuts at: 616 5749 616
Size: 5133 776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1662 1774 2357 3734 4166 4913 5181 1662
Size: 112 583 1377 432 747 268 2390
Fragments arranged by size:
2390 1377 747 583 432 268 112
AflIII A'CryG_T
Cuts at: 1440 1830 3261 4365 5342 1440
Size: 390 1431 1104 977 2007
Fragments arranged by size:
2007 1431 1104 977 390
AgeI A'CCGG_T
Cuts at: 4297 4297
Size: 5909
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2152 2152
Size: 5909
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 567 599 2396 2428 567
Size: 32 1797 32 4048
Fragments arranged by size:
4048 1797 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2845 3214 4781 2845
Size: 369 1567 3973
Fragments arranged by size:
3973 1567 369
ApaI G_GGCC'C
Cuts at: 2473 2473
Size: 5909
ApaLI G'TGCA_C
Cuts at: 4679 4679
Size: 5909
ApoI r'AATT_y
Cuts at: 906 2478 2913 5262 5518 906
Size: 1572 435 2349 256 1297
Fragments arranged by size:
2349 1572 1297 435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5909
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5909
AvaI C'yCGr_G
Cuts at: 2514 2825 2929 2514
Size: 311 104 5494
Fragments arranged by size:
5494 311 104
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 458 491 458
Size: 33 5876
BamHI G'GATC_C
Cuts at: 2934 2934
Size: 5909
BanI G'GyrC_C
Cuts at: 864 2925 3339 3587 3622 864
Size: 2061 414 248 35 3151
Fragments arranged by size:
3151 2061 414 248 35
BanII G_rGCy'C
Cuts at: 613 1088 2473 2923 3953 613
Size: 475 1385 450 1030 2569
Fragments arranged by size:
2569 1385 1030 475 450
BbeI G_GCGC'C
Cuts at: 3343 3591 3343
Size: 248 5661
BciVI GTATCCnnnnn_n'
Cuts at: 486 3807 4568 5909 486
Size: 3321 761 1341 486
Fragments arranged by size:
3321 1341 761 486
BclI T'GATC_A
Cuts at: 3429 3429
Size: 5909
BglI GCCn_nnn'nGGC
Cuts at: 1353 2812 1353
Size: 1459 4450
BglII A'GATC_T
Cuts at: 3424 5461 3424
Size: 2037 3872
BlpI GC'TnA_GC
Cuts at: 3021 3021
Size: 5909
Bme1580I G_kGCm'C
Cuts at: 779 1746 2473 2553 3534 3627 4683 779
Size: 967 727 80 981 93 1056 2005
Fragments arranged by size:
2005 1056 981 967 727 93 80
BmrI ACTGGGnnnn_n'
Cuts at: 235 494 784 1343 1804 2090 2180 3121 3202
Size: 259 290 559 461 286 90 941 81
Cuts at: 3202 3537 5681 235
Size: 335 2144 463
Fragments arranged by size:
2144 941 559 463 461 335 290 286
259 90 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4276 4276
Size: 5909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 914 1283 2309 728
Size: 186 369 1026 4328
Fragments arranged by size:
4328 1026 369 186
Bpu10I CC'TnA_GC
Cuts at: 438 2502 438
Size: 2064 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 3085 3964 4456 4754 1151
Size: 1934 879 492 298 2306
Fragments arranged by size:
2306 1934 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 139 5613 139
Size: 5474 435
BsaAI yAC'GTr
Cuts at: 1903 3262 1903
Size: 1359 4550
BsaBI GATnn'nnATC
Cuts at: 6 3423 5460 6
Size: 3417 2037 455
Fragments arranged by size:
3417 2037 455
BsaHI Gr'CG_yC
Cuts at: 3340 3588 4290 5786 3340
Size: 248 702 1496 3463
Fragments arranged by size:
3463 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3098 3619 4297 4571 4718 5129 3098
Size: 521 678 274 147 411 3878
Fragments arranged by size:
3878 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 567 597 630 660 744 774 567
Size: 30 33 30 84 30 5702
Fragments arranged by size:
5702 84 33 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 572 731 1310 2312 572
Size: 159 579 1002 4169
Fragments arranged by size:
4169 1002 579 159
BseYI C'CCAG_C
Cuts at: 2125 3334 4092 4669 5165 2125
Size: 1209 758 577 496 2869
Fragments arranged by size:
2869 1209 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 2135 2180 2210 2225 2120
Size: 15 45 30 15 5804
Fragments arranged by size:
5804 45 30 15 15
BsiEI CG_ry'CG
Cuts at: 104 3451 3497 4705 104
Size: 3347 46 1208 1308
Fragments arranged by size:
3347 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 900 2923 3701 3891 4683 900
Size: 2023 778 190 792 2126
Fragments arranged by size:
2126 2023 792 778 190
BsmI GAATG_Cn'
Cuts at: 1043 1205 2066 2186 1043
Size: 162 861 120 4766
Fragments arranged by size:
4766 861 162 120
Bsp1286I G_dGCh'C
Cuts at: 613 779 900 1088 1746 2473 2553 2923 3534
Size: 166 121 188 658 727 80 370 611
Cuts at: 3534 3627 3701 3891 3953 4683 613
Size: 93 74 190 62 730 1839
Fragments arranged by size:
1839 730 727 658 611 370 190 188
166 121 93 80 74 62
BspEI T'CCGG_A
Cuts at: 5129 5129
Size: 5909
BspHI T'CATG_A
Cuts at: 528 5085 528
Size: 4557 1352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 882 2959 3173 3475 3856 882
Size: 2077 214 302 381 2935
Fragments arranged by size:
2935 2077 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4201 4255 5739 5904 50
Size: 4151 54 1484 165 55
Fragments arranged by size:
4151 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 754 1058 3821 754
Size: 304 2763 2842
Fragments arranged by size:
2842 2763 304
BsrFI r'CCGG_y
Cuts at: 3907 4088 4297 3907
Size: 181 209 5519
Fragments arranged by size:
5519 209 181
BsrGI T'GTAC_A
Cuts at: 1827 1827
Size: 5909
BssSI C'ACGA_G
Cuts at: 4180 4538 4180
Size: 358 5551
BstAPI GCAn_nnn'nTGC
Cuts at: 1038 2694 3286 1038
Size: 1656 592 3661
Fragments arranged by size:
3661 1656 592
BstBI TT'CG_AA
Cuts at: 1659 4270 1659
Size: 2611 3298
BstXI CCAn_nnnn'nTGG
Cuts at: 114 2629 114
Size: 2515 3394
BstYI r'GATC_y
Cuts at: 1 2934 3401 3424 3759 4005 5017 5103 5115
Size: 2933 467 23 335 246 1012 86 12
Cuts at: 5115 5461 1
Size: 346 449
Fragments arranged by size:
2933 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 155 2807 107
Size: 48 2652 3209
Fragments arranged by size:
3209 2652 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 706 3859 4040 4203 706
Size: 3153 181 163 2412
Fragments arranged by size:
3153 2412 181 163
BtsI GCAGTG_nn'
Cuts at: 817 990 1106 3152 817
Size: 173 116 2046 3574
Fragments arranged by size:
3574 2046 173 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2427 2462 2427
Size: 35 5874
DraI TTT'AAA
Cuts at: 1458 1884 2771 2908 1458
Size: 426 887 137 4459
Fragments arranged by size:
4459 887 426 137
DraIII CAC_nnn'GTG
Cuts at: 502 502
Size: 5909
DrdI GACnn_nn'nnGTC
Cuts at: 3615 4473 3615
Size: 858 5051
EaeI y'GGCC_r
Cuts at: 3494 3668 4059 4086 5431 3494
Size: 174 391 27 1345 3972
Fragments arranged by size:
3972 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3494 3494
Size: 5909
EarI CTCTTCn'nnn_
Cuts at: 2210 3932 4142 2210
Size: 1722 210 3977
Fragments arranged by size:
3977 1722 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 842 4117 4427 4573 5719 842
Size: 3275 310 146 1146 1032
Fragments arranged by size:
3275 1146 1032 310 146
EcoICRI GAG'CTC
Cuts at: 2921 2921
Size: 5909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 914 1283 1662 1774 2309 2357 3734 4166
Size: 186 369 379 112 535 48 1377 432
Cuts at: 4166 4913 5181 728
Size: 747 268 1456
Fragments arranged by size:
1456 1377 747 535 432 379 369 268
186 112 48
EcoNI CCTnn'n_nnAGG
Cuts at: 437 2006 2036 2204 2324 2399 437
Size: 1569 30 168 120 75 3947
Fragments arranged by size:
3947 1569 168 120 75 30
EcoO109I rG'GnC_Cy
Cuts at: 1294 2342 2469 2470 3048 1294
Size: 1048 127 1 578 4155
Fragments arranged by size:
4155 1048 578 127 1
EcoRI G'AATT_C
Cuts at: 2913 2913
Size: 5909
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 3928 2908
Size: 1020 4889
FspI TGC'GCA
Cuts at: 283 3690 283
Size: 3407 2502
FspAI rTGC'GCAy
Cuts at: 283 283
Size: 5909
HaeII r_GCGC'y
Cuts at: 3343 3591 4613 5592 3343
Size: 248 1022 979 3660
Fragments arranged by size:
3660 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 684 716 960 992 1256 1288 1692 1711 1724
Size: 32 244 32 264 32 404 19 13
Cuts at: 1724 1743 3751 3781 3783 3813 684
Size: 19 2008 30 2 30 2780
Fragments arranged by size:
2780 2008 404 264 244 32 32 32
30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 957 1725 2948 957
Size: 768 1223 3918
Fragments arranged by size:
3918 1223 768
HindIII A'AGCT_T
Cuts at: 1181 1946 1181
Size: 765 5144
HpaI GTT'AAC
Cuts at: 957 957
Size: 5909
KasI G'GCGC_C
Cuts at: 3339 3587 3339
Size: 248 5661
KpnI G_GTAC'C
Cuts at: 2929 2929
Size: 5909
MfeI C'AATT_G
Cuts at: 140 140
Size: 5909
MluI A'CGCG_T
Cuts at: 5342 5342
Size: 5909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 503 2358 4580 4764 503
Size: 1855 2222 184 1648
Fragments arranged by size:
2222 1855 1648 184
MscI TGG'CCA
Cuts at: 3670 3670
Size: 5909
MslI CAynn'nnrTG
Cuts at: 112 2564 2627 3898 4025 112
Size: 2452 63 1271 127 1996
Fragments arranged by size:
2452 1996 1271 127 63
MspA1I CmG'CkG
Cuts at: 2842 3020 3334 3694 4707 4952 2842
Size: 178 314 360 1013 245 3799
Fragments arranged by size:
3799 1013 360 314 245 178
NaeI GCC'GGC
Cuts at: 4090 4090
Size: 5909
NarI GG'CG_CC
Cuts at: 3340 3588 3340
Size: 248 5661
NcoI C'CATG_G
Cuts at: 107 155 2807 107
Size: 48 2652 3209
Fragments arranged by size:
3209 2652 48
NgoMIV G'CCGG_C
Cuts at: 4088 4088
Size: 5909
NspI r_CATG'y
Cuts at: 840 1444 1834 2224 2697 2962 3462 4369 840
Size: 604 390 390 473 265 500 907 2380
Fragments arranged by size:
2380 907 604 500 473 390 390 265
PasI CC'CwG_GG
Cuts at: 2554 2554
Size: 5909
PciI A'CATG_T
Cuts at: 1440 1830 4365 1440
Size: 390 2535 2984
Fragments arranged by size:
2984 2535 390
PflMI CCAn_nnn'nTGG
Cuts at: 2581 2581
Size: 5909
PfoI T'CCnGG_A
Cuts at: 2055 2325 2055
Size: 270 5639
PmeI GTTT'AAAC
Cuts at: 2908 2908
Size: 5909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 567 599 5048 5080 567
Size: 32 4449 32 1396
Fragments arranged by size:
4449 1396 32 32
PpuMI rG'GwC_Cy
Cuts at: 1294 1294
Size: 5909
PspOMI G'GGCC_C
Cuts at: 2469 2469
Size: 5909
PstI C_TGCA'G
Cuts at: 1673 2052 2956 3641 1673
Size: 379 904 685 3941
Fragments arranged by size:
3941 904 685 379
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5909
PvuII CAG'CTG
Cuts at: 2842 3334 3694 2842
Size: 492 360 5057
Fragments arranged by size:
5057 492 360
RsrII CG'GwC_CG
Cuts at: 4104 4104
Size: 5909
SacI G_AGCT'C
Cuts at: 2923 2923
Size: 5909
SalI G'TCGA_C
Cuts at: 2946 2946
Size: 5909
SapI GCTCTTCn'nnn_
Cuts at: 2210 3932 4142 2210
Size: 1722 210 3977
Fragments arranged by size:
3977 1722 210
SbfI CC_TGCA'GG
Cuts at: 2956 2956
Size: 5909
ScaI AGT'ACT
Cuts at: 5426 5426
Size: 5909
SfcI C'TryA_G
Cuts at: 33 1669 2048 2234 2870 2952 3637 4630 4821
Size: 1636 379 186 636 82 685 993 191
Cuts at: 4821 33
Size: 1121
Fragments arranged by size:
1636 1121 993 685 636 379 191 186
82
SfoI GGC'GCC
Cuts at: 3341 3589 3341
Size: 248 5661
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5909
SmaI CCC'GGG
Cuts at: 2516 2931 2516
Size: 415 5494
SmlI C'TyrA_G
Cuts at: 1166 3064 3979 4471 4733 1166
Size: 1898 915 492 262 2342
Fragments arranged by size:
2342 1898 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5909
SphI G_CATG'C
Cuts at: 840 2224 2962 3462 840
Size: 1384 738 500 3287
Fragments arranged by size:
3287 1384 738 500
SspI AAT'ATT
Cuts at: 1758 1758
Size: 5909
StuI AGG'CCT
Cuts at: 2120 2361 2868 2120
Size: 241 507 5161
Fragments arranged by size:
5161 507 241
StyI C'CwwG_G
Cuts at: 107 155 2007 2807 3043 3397 107
Size: 48 1852 800 236 354 2619
Fragments arranged by size:
2619 1852 800 354 236 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 513 822 840 2554 2685 3492 3625 4084
Size: 296 309 18 1714 131 807 133 459
Cuts at: 4084 4293 217
Size: 209 1833
Fragments arranged by size:
1833 1714 807 459 309 296 209 133
131 18
TatI w'GTAC_w
Cuts at: 1586 1827 5424 1586
Size: 241 3597 2071
Fragments arranged by size:
3597 2071 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 630 662 1796 1828 5426 5458 630
Size: 32 1134 32 3598 32 1081
Fragments arranged by size:
3598 1134 1081 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3706 3706
Size: 5909
XbaI T'CTAG_A
Cuts at: 2940 2940
Size: 5909
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 175 3404 175
Size: 3229 2680
XmaI C'CCGG_G
Cuts at: 2514 2929 2514
Size: 415 5494
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI