Restriction map for
pF1KA0761
(Circular) (Six-base) MAPSORT of: pF1KA0761.seq Check: 1513 from: 1 to: 4766
pF1KA0761 4766 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1276 1276
Size: 4766
AccI GT'mk_AC
Cuts at: 1804 1804
Size: 4766
Acc65I G'GTAC_C
Cuts at: 1482 1782 1482
Size: 300 4466
AclI AA'CG_TT
Cuts at: 4606 4606
Size: 4766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 854 1445 1685 2591 3023 3770 4038 197
Size: 657 591 240 906 432 747 268 925
Fragments arranged by size:
925 906 747 657 591 432 268 240
AfeI AGC'GCT
Cuts at: 1103 1103
Size: 4766
AflIII A'CryG_T
Cuts at: 750 2118 3222 4199 750
Size: 1368 1104 977 1317
Fragments arranged by size:
1368 1317 1104 977
AgeI A'CCGG_T
Cuts at: 3154 3154
Size: 4766
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 494 494
Size: 4766
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1160 1192 1160
Size: 32 4734
AlwNI CAG_nnn'CTG
Cuts at: 1754 2071 3638 1754
Size: 317 1567 2882
Fragments arranged by size:
2882 1567 317
ApaI G_GGCC'C
Cuts at: 1324 1324
Size: 4766
ApaLI G'TGCA_C
Cuts at: 3536 3536
Size: 4766
ApoI r'AATT_y
Cuts at: 1069 1094 1770 4119 4375 1069
Size: 25 676 2349 256 1460
Fragments arranged by size:
2349 1460 676 256 25
AseI AT'TA_AT
Cuts at: 20 587 20
Size: 567 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 1786 1786
Size: 4766
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1466 1499 1466
Size: 33 4733
BamHI G'GATC_C
Cuts at: 648 1737 1791 648
Size: 1089 54 3623
Fragments arranged by size:
3623 1089 54
BanI G'GyrC_C
Cuts at: 553 1482 1782 2196 2444 2479 553
Size: 929 300 414 248 35 2840
Fragments arranged by size:
2840 929 414 300 248 35
BanII G_rGCy'C
Cuts at: 962 1324 1423 1780 2810 962
Size: 362 99 357 1030 2918
Fragments arranged by size:
2918 1030 362 357 99
BbeI G_GCGC'C
Cuts at: 2200 2448 2200
Size: 248 4518
BbsI GAAGACnn'nnnn_
Cuts at: 374 545 1251 374
Size: 171 706 3889
Fragments arranged by size:
3889 706 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4732
BciVI GTATCCnnnnn_n'
Cuts at: 1503 2664 3425 4766 1503
Size: 1161 761 1341 1503
Fragments arranged by size:
1503 1341 1161 761
BclI T'GATC_A
Cuts at: 2286 2286
Size: 4766
BfrBI ATG'CAT
Cuts at: 471 471
Size: 4766
BglI GCCn_nnn'nGGC
Cuts at: 1319 1757 1319
Size: 438 4328
BglII A'GATC_T
Cuts at: 2281 4318 2281
Size: 2037 2729
BlpI GC'TnA_GC
Cuts at: 1878 1878
Size: 4766
Bme1580I G_kGCm'C
Cuts at: 1324 2391 2484 3540 1324
Size: 1067 93 1056 2550
Fragments arranged by size:
2550 1067 1056 93
BmgBI CAC'GTC
Cuts at: 751 751
Size: 4766
BmrI ACTGGGnnnn_n'
Cuts at: 1073 1481 1978 2059 2394 4538 1073
Size: 408 497 81 335 2144 1301
Fragments arranged by size:
2144 1301 497 408 335 81
BmtI G_CTAG'C
Cuts at: 814 814
Size: 4766
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 248 1232 3133 248
Size: 984 1901 1881
Fragments arranged by size:
1901 1881 984
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1416 1642 1942 2821 3313 3611 1416
Size: 226 300 879 492 298 2571
Fragments arranged by size:
2571 879 492 300 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 4470 4470
Size: 4766
BsaAI yAC'GTr
Cuts at: 1729 2119 1729
Size: 390 4376
BsaBI GATnn'nnATC
Cuts at: 6 2280 4317 6
Size: 2274 2037 455
Fragments arranged by size:
2274 2037 455
BsaHI Gr'CG_yC
Cuts at: 2197 2445 3147 4643 2197
Size: 248 702 1496 2320
Fragments arranged by size:
2320 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1740 1955 2476 3154 3428 3575 3986 1740
Size: 215 521 678 274 147 411 2520
Fragments arranged by size:
2520 678 521 411 274 215 147
BseYI C'CCAG_C
Cuts at: 1652 2191 2949 3526 4022 1652
Size: 539 758 577 496 2396
Fragments arranged by size:
2396 758 577 539 496
BsiEI CG_ry'CG
Cuts at: 104 2308 2354 3562 104
Size: 2204 46 1208 1308
Fragments arranged by size:
2204 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 962 1433 1780 2558 2748 3540 962
Size: 471 347 778 190 792 2188
Fragments arranged by size:
2188 792 778 471 347 190
BsmI GAATG_Cn'
Cuts at: 139 139
Size: 4766
BsmBI CGTCTCn'nnnn_
Cuts at: 741 741
Size: 4766
Bsp1286I G_dGCh'C
Cuts at: 962 1324 1423 1433 1780 2391 2484 2558 2748
Size: 362 99 10 347 611 93 74 190
Cuts at: 2748 2810 3540 962
Size: 62 730 2188
Fragments arranged by size:
2188 730 611 362 347 190 99 93
74 62 10
BspEI T'CCGG_A
Cuts at: 3986 3986
Size: 4766
BspHI T'CATG_A
Cuts at: 162 600 1085 3942 162
Size: 438 485 2857 986
Fragments arranged by size:
2857 986 485 438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1816 2030 2332 2713 1276
Size: 540 214 302 381 3329
Fragments arranged by size:
3329 540 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3058 3112 4596 4761 50
Size: 3008 54 1484 165 55
Fragments arranged by size:
3008 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2678 2678
Size: 4766
BsrFI r'CCGG_y
Cuts at: 2764 2945 3154 2764
Size: 181 209 4376
Fragments arranged by size:
4376 209 181
BssSI C'ACGA_G
Cuts at: 1463 3037 3395 1463
Size: 1574 358 2834
Fragments arranged by size:
2834 1574 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1291 2143 1291
Size: 852 3914
BstBI TT'CG_AA
Cuts at: 629 3127 629
Size: 2498 2268
BstXI CCAn_nnnn'nTGG
Cuts at: 879 879
Size: 4766
BstYI r'GATC_y
Cuts at: 1 648 1737 1791 2258 2281 2616 2862 3874
Size: 647 1089 54 467 23 335 246 1012
Cuts at: 3874 3960 3972 4318 1
Size: 86 12 346 449
Fragments arranged by size:
1089 1012 647 467 449 346 335 246
86 54 23 12
BtgI C'CryG_G
Cuts at: 1234 1475 1234
Size: 241 4525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2716 2897 3060 2716
Size: 181 163 4422
Fragments arranged by size:
4422 181 163
BtsI GCAGTG_nn'
Cuts at: 2009 2009
Size: 4766
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1697 1732 1697
Size: 35 4731
DraI TTT'AAA
Cuts at: 1765 1765
Size: 4766
DraIII CAC_nnn'GTG
Cuts at: 574 574
Size: 4766
DrdI GACnn_nn'nnGTC
Cuts at: 2472 3330 2472
Size: 858 3908
EaeI y'GGCC_r
Cuts at: 1231 1586 1602 2351 2525 2916 2943 4288 1231
Size: 355 16 749 174 391 27 1345 1709
Fragments arranged by size:
1709 1345 749 391 355 174 27 16
EagI C'GGCC_G
Cuts at: 2351 2351
Size: 4766
EarI CTCTTCn'nnn_
Cuts at: 1359 2789 2999 1359
Size: 1430 210 3126
Fragments arranged by size:
3126 1430 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2974 3284 3430 4576 2974
Size: 310 146 1146 3164
Fragments arranged by size:
3164 1146 310 146
EcoICRI GAG'CTC
Cuts at: 960 1778 960
Size: 818 3948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 854 1445 1685 2591 3023 3770 4038 197
Size: 657 591 240 906 432 747 268 925
Fragments arranged by size:
925 906 747 657 591 432 268 240
EcoO109I rG'GnC_Cy
Cuts at: 1321 1336 1905 1321
Size: 15 569 4182
Fragments arranged by size:
4182 569 15
EcoRI G'AATT_C
Cuts at: 1770 1770
Size: 4766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 521 2785 521
Size: 2264 2502
FspI TGC'GCA
Cuts at: 2547 2547
Size: 4766
HaeII r_GCGC'y
Cuts at: 1105 2200 2448 3470 4449 1105
Size: 1095 248 1022 979 1422
Fragments arranged by size:
1422 1095 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 283 315 2608 2638 2640 2670 283
Size: 32 2293 30 2 30 2379
Fragments arranged by size:
2379 2293 32 30 30 2
HincII GTy'rAC
Cuts at: 972 1392 1805 972
Size: 420 413 3933
Fragments arranged by size:
3933 420 413
HindIII A'AGCT_T
Cuts at: 436 436
Size: 4766
KasI G'GCGC_C
Cuts at: 2196 2444 2196
Size: 248 4518
KpnI G_GTAC'C
Cuts at: 1486 1786 1486
Size: 300 4466
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3437 3621 3437
Size: 184 4582
MscI TGG'CCA
Cuts at: 1604 2527 1604
Size: 923 3843
MslI CAynn'nnrTG
Cuts at: 167 190 476 618 2755 2882 167
Size: 23 286 142 2137 127 2051
Fragments arranged by size:
2137 2051 286 142 127 23
MspA1I CmG'CkG
Cuts at: 1642 1877 2191 2551 3564 3809 1642
Size: 235 314 360 1013 245 2599
Fragments arranged by size:
2599 1013 360 314 245 235
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 4766
NarI GG'CG_CC
Cuts at: 2197 2445 2197
Size: 248 4518
NdeI CA'TA_TG
Cuts at: 723 723
Size: 4766
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 4766
NheI G'CTAG_C
Cuts at: 810 810
Size: 4766
NsiI A_TGCA'T
Cuts at: 473 473
Size: 4766
NspI r_CATG'y
Cuts at: 195 1819 2319 3226 195
Size: 1624 500 907 1735
Fragments arranged by size:
1735 1624 907 500
PacI TTA_AT'TAA
Cuts at: 587 587
Size: 4766
PciI A'CATG_T
Cuts at: 3222 3222
Size: 4766
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PmlI CAC'GTG
Cuts at: 1729 1729
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4734
PsiI TTA'TAA
Cuts at: 335 335
Size: 4766
PspOMI G'GGCC_C
Cuts at: 1320 1320
Size: 4766
PstI C_TGCA'G
Cuts at: 1813 2498 1813
Size: 685 4081
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 2191 2551 2191
Size: 360 4406
RsrII CG'GwC_CG
Cuts at: 2961 2961
Size: 4766
SacI G_AGCT'C
Cuts at: 962 1780 962
Size: 818 3948
SalI G'TCGA_C
Cuts at: 1803 1803
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 2789 2999 2789
Size: 210 4556
SbfI CC_TGCA'GG
Cuts at: 1813 1813
Size: 4766
ScaI AGT'ACT
Cuts at: 1569 4283 1569
Size: 2714 2052
SexAI A'CCwGG_T
Cuts at: 549 549
Size: 4766
SfcI C'TryA_G
Cuts at: 33 1809 2494 3487 3678 33
Size: 1776 685 993 191 1121
Fragments arranged by size:
1776 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1319 1319
Size: 4766
SfoI GGC'GCC
Cuts at: 2198 2446 2198
Size: 248 4518
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1788 1788
Size: 4766
SmlI C'TyrA_G
Cuts at: 1395 1657 1921 2836 3328 3590 1395
Size: 262 264 915 492 262 2571
Fragments arranged by size:
2571 915 492 264 262 262
SpeI A'CTAG_T
Cuts at: 67 966 67
Size: 899 3867
SphI G_CATG'C
Cuts at: 1819 2319 1819
Size: 500 4266
StyI C'CwwG_G
Cuts at: 557 1520 1686 1900 2254 557
Size: 963 166 214 354 3069
Fragments arranged by size:
3069 963 354 214 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2349 2482 2941 3150 2349
Size: 133 459 209 3965
Fragments arranged by size:
3965 459 209 133
TatI w'GTAC_w
Cuts at: 1567 4281 1567
Size: 2714 2052
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 537 569 4283 4315 537
Size: 32 3714 32 988
Fragments arranged by size:
3714 988 32 32
Tth111I GACn'n_nGTC
Cuts at: 2563 2563
Size: 4766
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2261 2261
Size: 4766
XmaI C'CCGG_G
Cuts at: 1786 1786
Size: 4766
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NheI NsiI NspI PacI PciI PmeI
PmlI PpiI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BbvCI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NotI NruI PasI PflMI PfoI PpuMI
PshAI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI