Restriction map for
pF1KA0751
(Circular) (Six-base) MAPSORT of: pF1KA0751.seq Check: 6392 from: 1 to: 6677
pF1KA0751 6677 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2844 3213 2844
Size: 369 6308
AccI GT'mk_AC
Cuts at: 3061 3715 3061
Size: 654 6023
Acc65I G'GTAC_C
Cuts at: 3693 3693
Size: 6677
AclI AA'CG_TT
Cuts at: 2947 6517 2947
Size: 3570 3107
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 2212 4502 4934 5681 5949 623
Size: 1589 2290 432 747 268 1351
Fragments arranged by size:
2290 1589 1351 747 432 268
AflIII A'CryG_T
Cuts at: 1318 4029 5133 6110 1318
Size: 2711 1104 977 1885
Fragments arranged by size:
2711 1885 1104 977
AgeI A'CCGG_T
Cuts at: 5065 5065
Size: 6677
AhdI GACnn_n'nnGTC
Cuts at: 2632 2632
Size: 6677
AlwNI CAG_nnn'CTG
Cuts at: 3195 3982 5549 3195
Size: 787 1567 4323
Fragments arranged by size:
4323 1567 787
ApaI G_GGCC'C
Cuts at: 3276 3276
Size: 6677
ApaLI G'TGCA_C
Cuts at: 5447 5447
Size: 6677
ApoI r'AATT_y
Cuts at: 543 1976 2092 2158 3681 6030 6286 543
Size: 1433 116 66 1523 2349 256 934
Fragments arranged by size:
2349 1523 1433 934 256 116 66
AseI AT'TA_AT
Cuts at: 20 1903 2806 20
Size: 1883 903 3891
Fragments arranged by size:
3891 1883 903
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
AvaI C'yCGr_G
Cuts at: 611 776 1454 3697 611
Size: 165 678 2243 3591
Fragments arranged by size:
3591 2243 678 165
BamHI G'GATC_C
Cuts at: 593 3702 593
Size: 3109 3568
BanI G'GyrC_C
Cuts at: 3693 4107 4355 4390 3693
Size: 414 248 35 5980
Fragments arranged by size:
5980 414 248 35
BanII G_rGCy'C
Cuts at: 2308 3276 3691 4721 2308
Size: 968 415 1030 4264
Fragments arranged by size:
4264 1030 968 415
BbeI G_GCGC'C
Cuts at: 4111 4359 4111
Size: 248 6429
BbsI GAAGACnn'nnnn_
Cuts at: 395 1617 2292 395
Size: 1222 675 4780
Fragments arranged by size:
4780 1222 675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1184 1218 90
Size: 34 1060 34 5549
Fragments arranged by size:
5549 1060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 312 4575 5336 6677 312
Size: 4263 761 1341 312
Fragments arranged by size:
4263 1341 761 312
BclI T'GATC_A
Cuts at: 396 4197 396
Size: 3801 2876
BglII A'GATC_T
Cuts at: 334 715 1929 2415 2818 4192 6229 334
Size: 381 1214 486 403 1374 2037 782
Fragments arranged by size:
2037 1374 1214 782 486 403 381
BlpI GC'TnA_GC
Cuts at: 3173 3789 3173
Size: 616 6061
Bme1580I G_kGCm'C
Cuts at: 3276 4302 4395 5451 3276
Size: 1026 93 1056 4502
Fragments arranged by size:
4502 1056 1026 93
BmrI ACTGGGnnnn_n'
Cuts at: 2525 2873 3889 3970 4305 6449 2525
Size: 348 1016 81 335 2144 2753
Fragments arranged by size:
2753 2144 1016 348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5044 5044
Size: 6677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1768 2060 2639 2739 2879 3274 1768
Size: 292 579 100 140 395 5171
Fragments arranged by size:
5171 579 395 292 140 100
Bpu10I CC'TnA_GC
Cuts at: 688 785 688
Size: 97 6580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 894 2755 3853 4732 5224 5522 894
Size: 1861 1098 879 492 298 2049
Fragments arranged by size:
2049 1861 1098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 999 2489 2855 6381 999
Size: 1490 366 3526 1295
Fragments arranged by size:
3526 1490 1295 366
BsaAI yAC'GTr
Cuts at: 1319 4030 1319
Size: 2711 3966
BsaBI GATnn'nnATC
Cuts at: 6 339 720 1397 2709 4191 6228 6
Size: 333 381 677 1312 1482 2037 455
Fragments arranged by size:
2037 1482 1312 677 455 381 333
BsaHI Gr'CG_yC
Cuts at: 3186 4108 4356 5058 6554 3186
Size: 922 248 702 1496 3309
Fragments arranged by size:
3309 1496 922 702 248
BsaWI w'CCGG_w
Cuts at: 2593 3866 4387 5065 5339 5486 5897 2593
Size: 1273 521 678 274 147 411 3373
Fragments arranged by size:
3373 1273 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2714 2744 3588 3618 2714
Size: 30 844 30 5773
Fragments arranged by size:
5773 844 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 858 1093 1357 2477 2531 3596 858
Size: 235 264 1120 54 1065 3939
Fragments arranged by size:
3939 1120 1065 264 235 54
BseYI C'CCAG_C
Cuts at: 4102 4860 5437 5933 4102
Size: 758 577 496 4846
Fragments arranged by size:
4846 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 4219 4265 5473 104
Size: 4115 46 1208 1308
Fragments arranged by size:
4115 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 618 2986 3691 4469 4659 5451 618
Size: 2368 705 778 190 792 1844
Fragments arranged by size:
2368 1844 792 778 705 190
BsmI GAATG_Cn'
Cuts at: 438 2111 438
Size: 1673 5004
Bsp1286I G_dGCh'C
Cuts at: 618 2308 2986 3276 3691 4302 4395 4469 4659
Size: 1690 678 290 415 611 93 74 190
Cuts at: 4659 4721 5451 618
Size: 62 730 1844
Fragments arranged by size:
1844 1690 730 678 611 415 290 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5897 5897
Size: 6677
BspHI T'CATG_A
Cuts at: 2786 5853 2786
Size: 3067 3610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2844 3213 3727 3941 4243 4624 2844
Size: 369 514 214 302 381 4897
Fragments arranged by size:
4897 514 381 369 302 214
BsrBI CCG'CTC
Cuts at: 50 2739 2753 4969 5023 6507 6672 50
Size: 2689 14 2216 54 1484 165 55
Fragments arranged by size:
2689 2216 1484 165 55 54 14
BsrDI GCAATG_nn'
Cuts at: 121 332 1475 2248 3215 4589 121
Size: 211 1143 773 967 1374 2209
Fragments arranged by size:
2209 1374 1143 967 773 211
BsrFI r'CCGG_y
Cuts at: 4675 4856 5065 4675
Size: 181 209 6287
Fragments arranged by size:
6287 209 181
BsrGI T'GTAC_A
Cuts at: 1643 1643
Size: 6677
BssSI C'ACGA_G
Cuts at: 2135 4948 5306 2135
Size: 2813 358 3506
Fragments arranged by size:
3506 2813 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3205 4054 3205
Size: 849 5828
BstBI TT'CG_AA
Cuts at: 3425 5038 3425
Size: 1613 5064
BstEII G'GTnAC_C
Cuts at: 1894 2525 1894
Size: 631 6046
BstYI r'GATC_y
Cuts at: 1 334 593 715 912 1929 2415 2704 2758
Size: 333 259 122 197 1017 486 289 54
Cuts at: 2758 2818 3591 3702 4169 4192 4527 4773 5785
Size: 60 773 111 467 23 335 246 1012
Cuts at: 5785 5871 5883 6229 1
Size: 86 12 346 449
Fragments arranged by size:
1017 1012 773 486 467 449 346 335
333 289 259 246 197 122 111 86
60 54 23 12
BtgI C'CryG_G
Cuts at: 740 2295 3017 3276 740
Size: 1555 722 259 4141
Fragments arranged by size:
4141 1555 722 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4627 4808 4971 4627
Size: 181 163 6333
Fragments arranged by size:
6333 181 163
BtsI GCAGTG_nn'
Cuts at: 3307 3920 3307
Size: 613 6064
DraI TTT'AAA
Cuts at: 3676 3676
Size: 6677
DraIII CAC_nnn'GTG
Cuts at: 2533 2533
Size: 6677
DrdI GACnn_nn'nnGTC
Cuts at: 2408 4383 5241 2408
Size: 1975 858 3844
Fragments arranged by size:
3844 1975 858
EaeI y'GGCC_r
Cuts at: 1254 3014 3477 4262 4436 4827 4854 6199 1254
Size: 1760 463 785 174 391 27 1345 1732
Fragments arranged by size:
1760 1732 1345 785 463 391 174 27
EagI C'GGCC_G
Cuts at: 4262 4262
Size: 6677
EarI CTCTTCn'nnn_
Cuts at: 506 1262 2082 2250 3422 3612 4700 4910 506
Size: 756 820 168 1172 190 1088 210 2273
Fragments arranged by size:
2273 1172 1088 820 756 210 190 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4885 5195 5341 6487 4885
Size: 310 146 1146 5075
Fragments arranged by size:
5075 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3689 3689
Size: 6677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 1768 2060 2212 2639 2739 2879 3274 4502
Size: 1145 292 152 427 100 140 395 1228
Cuts at: 4502 4934 5681 5949 623
Size: 432 747 268 1351
Fragments arranged by size:
1351 1228 1145 747 432 427 395 292
268 152 140 100
EcoNI CCTnn'n_nnAGG
Cuts at: 1339 2844 1339
Size: 1505 5172
EcoO109I rG'GnC_Cy
Cuts at: 2579 3816 2579
Size: 1237 5440
EcoRI G'AATT_C
Cuts at: 2158 3681 2158
Size: 1523 5154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1702 1982 3638 4696 1702
Size: 280 1656 1058 3683
Fragments arranged by size:
3683 1656 1058 280
FspI TGC'GCA
Cuts at: 4458 4458
Size: 6677
HaeII r_GCGC'y
Cuts at: 4111 4359 5381 6360 4111
Size: 248 1022 979 4428
Fragments arranged by size:
4428 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 346 378 505 537 688 720 904 936 2618
Size: 32 127 32 151 32 184 32 1682
Cuts at: 2618 2650 2838 2870 3478 3510 4519 4549 4551
Size: 32 188 32 608 32 1009 30 2
Cuts at: 4551 4581 346
Size: 30 2442
Fragments arranged by size:
2442 1682 1009 608 188 184 151 127
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3716 3716
Size: 6677
KasI G'GCGC_C
Cuts at: 4107 4355 4107
Size: 248 6429
KpnI G_GTAC'C
Cuts at: 3697 3697
Size: 6677
MluI A'CGCG_T
Cuts at: 6110 6110
Size: 6677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 1184 2533 2930 3619 5348 5532 443
Size: 741 1349 397 689 1729 184 1588
Fragments arranged by size:
1729 1588 1349 741 689 397 184
MscI TGG'CCA
Cuts at: 1256 4438 1256
Size: 3182 3495
MslI CAynn'nnrTG
Cuts at: 158 638 4666 4793 158
Size: 480 4028 127 2042
Fragments arranged by size:
4028 2042 480 127
MspA1I CmG'CkG
Cuts at: 3252 3788 4102 4462 5475 5720 3252
Size: 536 314 360 1013 245 4209
Fragments arranged by size:
4209 1013 536 360 314 245
NaeI GCC'GGC
Cuts at: 4858 4858
Size: 6677
NarI GG'CG_CC
Cuts at: 4108 4356 4108
Size: 248 6429
NcoI C'CATG_G
Cuts at: 740 2295 740
Size: 1555 5122
NgoMIV G'CCGG_C
Cuts at: 4856 4856
Size: 6677
NspI r_CATG'y
Cuts at: 1741 3730 4230 5137 1741
Size: 1989 500 907 3281
Fragments arranged by size:
3281 1989 907 500
PciI A'CATG_T
Cuts at: 5133 5133
Size: 6677
PflMI CCAn_nnn'nTGG
Cuts at: 1263 3195 1263
Size: 1932 4745
PfoI T'CCnGG_A
Cuts at: 673 1821 3152 673
Size: 1148 1331 4198
Fragments arranged by size:
4198 1331 1148
PmeI GTTT'AAAC
Cuts at: 3676 3676
Size: 6677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5816 5848 5816
Size: 32 6645
PpuMI rG'GwC_Cy
Cuts at: 2579 2579
Size: 6677
PsiI TTA'TAA
Cuts at: 2603 2603
Size: 6677
PspOMI G'GGCC_C
Cuts at: 3272 3272
Size: 6677
PstI C_TGCA'G
Cuts at: 3724 4409 3724
Size: 685 5992
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6677
PvuII CAG'CTG
Cuts at: 3252 4102 4462 3252
Size: 850 360 5467
Fragments arranged by size:
5467 850 360
RsrII CG'GwC_CG
Cuts at: 4872 4872
Size: 6677
SacI G_AGCT'C
Cuts at: 3691 3691
Size: 6677
SalI G'TCGA_C
Cuts at: 3714 3714
Size: 6677
SanDI GG'GwC_CC
Cuts at: 2579 2579
Size: 6677
SapI GCTCTTCn'nnn_
Cuts at: 3612 4700 4910 3612
Size: 1088 210 5379
Fragments arranged by size:
5379 1088 210
SbfI CC_TGCA'GG
Cuts at: 3724 3724
Size: 6677
ScaI AGT'ACT
Cuts at: 2892 6194 2892
Size: 3302 3375
SexAI A'CCwGG_T
Cuts at: 1216 1581 3294 1216
Size: 365 1713 4599
Fragments arranged by size:
4599 1713 365
SfcI C'TryA_G
Cuts at: 33 1565 3062 3084 3720 4405 5398 5589 33
Size: 1532 1497 22 636 685 993 191 1121
Fragments arranged by size:
1532 1497 1121 993 685 636 191 22
SfoI GGC'GCC
Cuts at: 4109 4357 4109
Size: 248 6429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
SmaI CCC'GGG
Cuts at: 3699 3699
Size: 6677
SmlI C'TyrA_G
Cuts at: 776 873 1454 2734 3832 4747 5239 5501 776
Size: 97 581 1280 1098 915 492 262 1952
Fragments arranged by size:
1952 1280 1098 915 581 492 262 97
SpeI A'CTAG_T
Cuts at: 67 1183 67
Size: 1116 5561
SphI G_CATG'C
Cuts at: 3730 4230 3730
Size: 500 6177
SspI AAT'ATT
Cuts at: 298 298
Size: 6677
StuI AGG'CCT
Cuts at: 1701 3011 1701
Size: 1310 5367
StyI C'CwwG_G
Cuts at: 740 1957 2295 3162 3599 3811 4165 740
Size: 1217 338 867 437 212 354 3252
Fragments arranged by size:
3252 1217 867 437 354 338 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2544 3202 4260 4393 4852 5061 2544
Size: 658 1058 133 459 209 4160
Fragments arranged by size:
4160 1058 658 459 209 133
TatI w'GTAC_w
Cuts at: 1244 1643 2890 2998 6192 1244
Size: 399 1247 108 3194 1729
Fragments arranged by size:
3194 1729 1247 399 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 2754 2786 6194 6226 2519
Size: 32 203 32 3408 32 2970
Fragments arranged by size:
3408 2970 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4474 4474
Size: 6677
XbaI T'CTAG_A
Cuts at: 1656 3708 1656
Size: 2052 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4172 4172
Size: 6677
XhoI C'TCGA_G
Cuts at: 776 1454 776
Size: 678 5999
XmaI C'CCGG_G
Cuts at: 3697 3697
Size: 6677
XmnI GAAnn'nnTTC
Cuts at: 3621 3621
Size: 6677
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmBI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SwaI ZraI