Restriction map for
pF1KA0750
(Circular) (Six-base) MAPSORT of: pF1KA0750.seq Check: 7415 from: 1 to: 6485
pF1KA0750 6485 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 761 761
Size: 6485
AccI GT'mk_AC
Cuts at: 3523 3523
Size: 6485
Acc65I G'GTAC_C
Cuts at: 187 1517 3501 187
Size: 1330 1984 3171
Fragments arranged by size:
3171 1984 1330
AclI AA'CG_TT
Cuts at: 6325 6325
Size: 6485
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 2891 3017 4310 4742 5489 5757 698
Size: 2193 126 1293 432 747 268 1426
Fragments arranged by size:
2193 1426 1293 747 432 268 126
AflIII A'CryG_T
Cuts at: 1496 3107 3109 3129 3837 4941 5918 1496
Size: 1611 2 20 708 1104 977 2063
Fragments arranged by size:
2063 1611 1104 977 708 20 2
AgeI A'CCGG_T
Cuts at: 4873 4873
Size: 6485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 195 2513 195
Size: 2318 4167
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 2931
Size: 32 6453
AlwNI CAG_nnn'CTG
Cuts at: 1029 2274 3200 3790 5357 1029
Size: 1245 926 590 1567 2157
Fragments arranged by size:
2157 1567 1245 926 590
ApaLI G'TGCA_C
Cuts at: 357 5255 357
Size: 4898 1587
ApoI r'AATT_y
Cuts at: 715 814 925 2571 2692 3253 3489 5838 6094
Size: 99 111 1646 121 561 236 2349 256
Cuts at: 6094 715
Size: 1106
Fragments arranged by size:
2349 1646 1106 561 256 236 121 111
99
AseI AT'TA_AT
Cuts at: 20 20
Size: 6485
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6485
AvaI C'yCGr_G
Cuts at: 1124 1600 2598 3505 1124
Size: 476 998 907 4104
Fragments arranged by size:
4104 998 907 476
BamHI G'GATC_C
Cuts at: 1874 3510 1874
Size: 1636 4849
BanI G'GyrC_C
Cuts at: 187 769 1399 1517 1682 1975 3418 3501 3915
Size: 582 630 118 165 293 1443 83 414
Cuts at: 3915 4163 4198 187
Size: 248 35 2474
Fragments arranged by size:
2474 1443 630 582 414 293 248 165
118 83 35
BanII G_rGCy'C
Cuts at: 1615 1793 1967 2203 2486 2673 3499 4529 1615
Size: 178 174 236 283 187 826 1030 3571
Fragments arranged by size:
3571 1030 826 283 236 187 178 174
BbeI G_GCGC'C
Cuts at: 3919 4167 3919
Size: 248 6237
BbvCI CC'TCA_GC
Cuts at: 2032 2951 2032
Size: 919 5566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2253 2287 2253
Size: 34 6451
BciVI GTATCCnnnnn_n'
Cuts at: 4383 5144 6485 4383
Size: 761 1341 4383
Fragments arranged by size:
4383 1341 761
BclI T'GATC_A
Cuts at: 3024 4005 3024
Size: 981 5504
BglI GCCn_nnn'nGGC
Cuts at: 1320 1320
Size: 6485
BglII A'GATC_T
Cuts at: 960 1624 4000 6037 960
Size: 664 2376 2037 1408
Fragments arranged by size:
2376 2037 1408 664
BlpI GC'TnA_GC
Cuts at: 1930 3151 3597 1930
Size: 1221 446 4818
Fragments arranged by size:
4818 1221 446
Bme1580I G_kGCm'C
Cuts at: 361 1344 1402 1978 2471 4110 4203 5259 361
Size: 983 58 576 493 1639 93 1056 1587
Fragments arranged by size:
1639 1587 1056 983 576 493 93 58
BmrI ACTGGGnnnn_n'
Cuts at: 1618 3433 3697 3778 4113 6257 1618
Size: 1815 264 81 335 2144 1846
Fragments arranged by size:
2144 1846 1815 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 4852 3398
Size: 1454 5031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1432 1606 1623 1865 2644 3038 178
Size: 1254 174 17 242 779 394 3625
Fragments arranged by size:
3625 1254 779 394 242 174 17
Bpu10I CC'TnA_GC
Cuts at: 1787 2032 2515 2951 1787
Size: 245 483 436 5321
Fragments arranged by size:
5321 483 436 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 985 1330 2470 3661 4540 5032 5330 250
Size: 735 345 1140 1191 879 492 298 1405
Fragments arranged by size:
1405 1191 1140 879 735 492 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 6189 6189
Size: 6485
BsaAI yAC'GTr
Cuts at: 179 2472 3110 3138 3838 179
Size: 2293 638 28 700 2826
Fragments arranged by size:
2826 2293 700 638 28
BsaBI GATnn'nnATC
Cuts at: 6 838 3999 6036 6
Size: 832 3161 2037 455
Fragments arranged by size:
3161 2037 832 455
BsaHI Gr'CG_yC
Cuts at: 758 3916 4164 4866 6362 758
Size: 3158 248 702 1496 881
Fragments arranged by size:
3158 1496 881 702 248
BsaWI w'CCGG_w
Cuts at: 2116 3674 4195 4873 5147 5294 5705 2116
Size: 1558 521 678 274 147 411 2896
Fragments arranged by size:
2896 1558 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 425 454 455 484 736 766 2719 2749 2787
Size: 29 1 29 252 30 1953 30 38
Cuts at: 2787 2817 425
Size: 30 4093
Fragments arranged by size:
4093 1953 252 38 30 30 30 29
29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1635 2302 2419 2420 2801 3352 3355 1635
Size: 667 117 1 381 551 3 4765
Fragments arranged by size:
4765 667 551 381 117 3 1
BseYI C'CCAG_C
Cuts at: 624 1663 2416 2556 2926 3910 4668 5245 5741
Size: 1039 753 140 370 984 758 577 496
Cuts at: 5741 624
Size: 1368
Fragments arranged by size:
1368 1039 984 758 753 577 496 370
140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 975 975
Size: 6485
BsiEI CG_ry'CG
Cuts at: 104 4027 4073 5281 104
Size: 3923 46 1208 1308
Fragments arranged by size:
3923 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 361 1592 1793 1967 2296 2362 2477 2486 3499
Size: 1231 201 174 329 66 115 9 1013
Cuts at: 3499 4277 4467 5259 361
Size: 778 190 792 1587
Fragments arranged by size:
1587 1231 1013 792 778 329 201 190
174 115 66 9
BsmBI CGTCTCn'nnnn_
Cuts at: 2136 2834 3303 2136
Size: 698 469 5318
Fragments arranged by size:
5318 698 469
Bsp1286I G_dGCh'C
Cuts at: 361 1344 1402 1592 1615 1793 1967 1978 2203
Size: 983 58 190 23 178 174 11 225
Cuts at: 2203 2296 2362 2471 2477 2486 2673 3499 4110
Size: 93 66 109 6 9 187 826 611
Cuts at: 4110 4203 4277 4467 4529 5259 361
Size: 93 74 190 62 730 1587
Fragments arranged by size:
1587 983 826 730 611 225 190 190
187 178 174 109 93 93 74 66
62 58 23 11 9 6
BspEI T'CCGG_A
Cuts at: 5705 5705
Size: 6485
BspHI T'CATG_A
Cuts at: 2312 5661 2312
Size: 3349 3136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 1118 1231 3535 3749 4051 4432 138
Size: 980 113 2304 214 302 381 2191
Fragments arranged by size:
2304 2191 980 381 302 214 113
BsrBI CCG'CTC
Cuts at: 50 1263 4777 4831 6315 6480 50
Size: 1213 3514 54 1484 165 55
Fragments arranged by size:
3514 1484 1213 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4397 4397
Size: 6485
BsrFI r'CCGG_y
Cuts at: 1442 2349 4483 4664 4873 1442
Size: 907 2134 181 209 3054
Fragments arranged by size:
3054 2134 907 209 181
BsrGI T'GTAC_A
Cuts at: 3014 3014
Size: 6485
BssSI C'ACGA_G
Cuts at: 1282 4756 5114 1282
Size: 3474 358 2653
Fragments arranged by size:
3474 2653 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1029 1141 1278 2510 3862 1029
Size: 112 137 1232 1352 3652
Fragments arranged by size:
3652 1352 1232 137 112
BstBI TT'CG_AA
Cuts at: 4846 4846
Size: 6485
BstEII G'GTnAC_C
Cuts at: 276 276
Size: 6485
BstYI r'GATC_y
Cuts at: 1 960 1624 1874 3510 3977 4000 4335 4581
Size: 959 664 250 1636 467 23 335 246
Cuts at: 4581 5593 5679 5691 6037 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1636 1012 959 664 467 449 346 335
250 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3035 3035
Size: 6485
BtgI C'CryG_G
Cuts at: 107 819 1325 1344 1889 1991 2098 2258 107
Size: 712 506 19 545 102 107 160 4334
Fragments arranged by size:
4334 712 545 506 160 107 102 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3257 4435 4616 4779 3257
Size: 1178 181 163 4963
Fragments arranged by size:
4963 1178 181 163
BtsI GCAGTG_nn'
Cuts at: 407 1752 2511 3410 3728 407
Size: 1345 759 899 318 3164
Fragments arranged by size:
3164 1345 899 759 318
DraI TTT'AAA
Cuts at: 3484 3484
Size: 6485
DraIII CAC_nnn'GTG
Cuts at: 371 1540 371
Size: 1169 5316
DrdI GACnn_nn'nnGTC
Cuts at: 1042 4191 5049 1042
Size: 3149 858 2478
Fragments arranged by size:
3149 2478 858
EaeI y'GGCC_r
Cuts at: 777 1205 1856 2039 2276 2681 3221 3407 4070
Size: 428 651 183 237 405 540 186 663
Cuts at: 4070 4244 4635 4662 6007 777
Size: 174 391 27 1345 1255
Fragments arranged by size:
1345 1255 663 651 540 428 405 391
237 186 183 174 27
EagI C'GGCC_G
Cuts at: 4070 4070
Size: 6485
EarI CTCTTCn'nnn_
Cuts at: 451 884 1378 1972 2083 2367 2659 3301 4508
Size: 433 494 594 111 284 292 642 1207
Cuts at: 4508 4718 451
Size: 210 2218
Fragments arranged by size:
2218 1207 642 594 494 433 292 284
210 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4693 5003 5149 6295 4693
Size: 310 146 1146 4883
Fragments arranged by size:
4883 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1791 1965 2484 3497 1791
Size: 174 519 1013 4779
Fragments arranged by size:
4779 1013 519 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 698 1432 1606 1623 1865 2644 2891 3017
Size: 520 734 174 17 242 779 247 126
Cuts at: 3017 3038 4310 4742 5489 5757 178
Size: 21 1272 432 747 268 906
Fragments arranged by size:
1272 906 779 747 734 520 432 268
247 242 174 126 21 17
EcoNI CCTnn'n_nnAGG
Cuts at: 607 607
Size: 6485
EcoO109I rG'GnC_Cy
Cuts at: 391 522 936 1547 3624 391
Size: 131 414 611 2077 3252
Fragments arranged by size:
3252 2077 611 414 131
EcoRI G'AATT_C
Cuts at: 814 3489 814
Size: 2675 3810
EcoRV GAT'ATC
Cuts at: 1656 1656
Size: 6485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3288 4504 3288
Size: 1216 5269
FspI TGC'GCA
Cuts at: 406 3402 4266 406
Size: 2996 864 2625
Fragments arranged by size:
2996 2625 864
HaeII r_GCGC'y
Cuts at: 3158 3919 4167 5189 6168 3158
Size: 761 248 1022 979 3475
Fragments arranged by size:
3475 1022 979 761 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 620 652 1891 1923 3049 3081 4327
Size: 32 134 32 1239 32 1126 32 1246
Cuts at: 4327 4357 4359 4389 454
Size: 30 2 30 2550
Fragments arranged by size:
2550 1246 1239 1126 134 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1632 1716 2868 3524 1632
Size: 84 1152 656 4593
Fragments arranged by size:
4593 1152 656 84
KasI G'GCGC_C
Cuts at: 3915 4163 3915
Size: 248 6237
KpnI G_GTAC'C
Cuts at: 191 1521 3505 191
Size: 1330 1984 3171
Fragments arranged by size:
3171 1984 1330
MluI A'CGCG_T
Cuts at: 5918 5918
Size: 6485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 1481 5156 5340 722
Size: 759 3675 184 1867
Fragments arranged by size:
3675 1867 759 184
MscI TGG'CCA
Cuts at: 1207 2041 2278 2683 4246 1207
Size: 834 237 405 1563 3446
Fragments arranged by size:
3446 1563 834 405 237
MslI CAynn'nnrTG
Cuts at: 177 644 1072 1939 4474 4601 177
Size: 467 428 867 2535 127 2061
Fragments arranged by size:
2535 2061 867 467 428 127
MspA1I CmG'CkG
Cuts at: 874 1158 1278 2271 2556 2842 2955 2978 2995
Size: 284 120 993 285 286 113 23 17
Cuts at: 2995 3191 3596 3910 4270 5283 5528 874
Size: 196 405 314 360 1013 245 1831
Fragments arranged by size:
1831 1013 993 405 360 314 286 285
284 245 196 120 113 23 17
NaeI GCC'GGC
Cuts at: 4666 4666
Size: 6485
NarI GG'CG_CC
Cuts at: 3916 4164 3916
Size: 248 6237
NcoI C'CATG_G
Cuts at: 107 1325 2258 107
Size: 1218 933 4334
Fragments arranged by size:
4334 1218 933
NgoMIV G'CCGG_C
Cuts at: 4664 4664
Size: 6485
NspI r_CATG'y
Cuts at: 1230 1714 3538 4038 4945 1230
Size: 484 1824 500 907 2770
Fragments arranged by size:
2770 1824 907 500 484
PasI CC'CwG_GG
Cuts at: 1507 1507
Size: 6485
PciI A'CATG_T
Cuts at: 4941 4941
Size: 6485
PflMI CCAn_nnn'nTGG
Cuts at: 1739 3475 1739
Size: 1736 4749
PfoI T'CCnGG_A
Cuts at: 472 1639 472
Size: 1167 5318
PmeI GTTT'AAAC
Cuts at: 3484 3484
Size: 6485
PmlI CAC'GTG
Cuts at: 179 2472 3110 179
Size: 2293 638 3554
Fragments arranged by size:
3554 2293 638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5624 5656 5624
Size: 32 6453
PpuMI rG'GwC_Cy
Cuts at: 391 936 391
Size: 545 5940
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 961 993 961
Size: 32 6453
PstI C_TGCA'G
Cuts at: 1137 2639 2994 3196 3532 4217 1137
Size: 1502 355 202 336 685 3405
Fragments arranged by size:
3405 1502 685 355 336 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6485
PvuII CAG'CTG
Cuts at: 1158 1278 2271 2556 2842 2955 2995 3910 4270
Size: 120 993 285 286 113 40 915 360
Cuts at: 4270 1158
Size: 3373
Fragments arranged by size:
3373 993 915 360 286 285 120 113
40
RsrII CG'GwC_CG
Cuts at: 4680 4680
Size: 6485
SacI G_AGCT'C
Cuts at: 1793 1967 2486 3499 1793
Size: 174 519 1013 4779
Fragments arranged by size:
4779 1013 519 174
SalI G'TCGA_C
Cuts at: 3522 3522
Size: 6485
SapI GCTCTTCn'nnn_
Cuts at: 1378 1972 2367 4508 4718 1378
Size: 594 395 2141 210 3145
Fragments arranged by size:
3145 2141 594 395 210
SbfI CC_TGCA'GG
Cuts at: 3532 3532
Size: 6485
ScaI AGT'ACT
Cuts at: 6002 6002
Size: 6485
SexAI A'CCwGG_T
Cuts at: 1678 1678
Size: 6485
SfcI C'TryA_G
Cuts at: 33 592 725 1133 2551 2635 2990 3192 3528
Size: 559 133 408 1418 84 355 202 336
Cuts at: 3528 4213 5206 5397 33
Size: 685 993 191 1121
Fragments arranged by size:
1418 1121 993 685 559 408 355 336
202 191 133 84
SfoI GGC'GCC
Cuts at: 3917 4165 3917
Size: 248 6237
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6485
SmaI CCC'GGG
Cuts at: 1126 3507 1126
Size: 2381 4104
SmlI C'TyrA_G
Cuts at: 265 964 1309 1600 2485 3640 4555 5047 5309
Size: 699 345 291 885 1155 915 492 262
Cuts at: 5309 265
Size: 1441
Fragments arranged by size:
1441 1155 915 885 699 492 345 291
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6485
SphI G_CATG'C
Cuts at: 3538 4038 3538
Size: 500 5985
StuI AGG'CCT
Cuts at: 199 2786 199
Size: 2587 3898
StyI C'CwwG_G
Cuts at: 107 272 881 1325 2083 2258 2524 3619 3973
Size: 165 609 444 758 175 266 1095 354
Cuts at: 3973 107
Size: 2619
Fragments arranged by size:
2619 1095 758 609 444 354 266 175
165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1009 1285 2430 2548 3482 4068 4201 4660 4869
Size: 276 1145 118 934 586 133 459 209
Cuts at: 4869 1009
Size: 2625
Fragments arranged by size:
2625 1145 934 586 459 276 209 133
118
TatI w'GTAC_w
Cuts at: 1493 3014 6000 1493
Size: 1521 2986 1978
Fragments arranged by size:
2986 1978 1521
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 425 457 540 572 2719 2751 6002 6034 425
Size: 32 83 32 2147 32 3251 32 876
Fragments arranged by size:
3251 2147 876 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4282 4282
Size: 6485
XbaI T'CTAG_A
Cuts at: 666 3516 666
Size: 2850 3635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 2193 3216 3420 3980 444
Size: 1749 1023 204 560 2949
Fragments arranged by size:
2949 1749 1023 560 204
XhoI C'TCGA_G
Cuts at: 1600 1600
Size: 6485
XmaI C'CCGG_G
Cuts at: 1124 3505 1124
Size: 2381 4104
XmnI GAAnn'nnTTC
Cuts at: 2152 2654 2152
Size: 502 5983
ZraI GAC'GTC
Cuts at: 759 759
Size: 6485
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BssHII
BstXI BstZ17I ClaI CspCI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI