Restriction map for
pF1KA0749
(Circular) (Six-base) MAPSORT of: pF1KA0749.seq Check: 8122 from: 1 to: 5084
pF1KA0749 5084 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 572 1356 572
Size: 784 4300
AatII G_ACGT'C
Cuts at: 788 788
Size: 5084
AccI GT'mk_AC
Cuts at: 369 2122 369
Size: 1753 3331
Acc65I G'GTAC_C
Cuts at: 2100 2100
Size: 5084
AclI AA'CG_TT
Cuts at: 4924 4924
Size: 5084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3341 3694 4356 2909
Size: 432 353 662 3637
Fragments arranged by size:
3637 662 432 353
AflIII A'CryG_T
Cuts at: 154 1395 2436 4236 4517 154
Size: 1241 1041 1800 281 721
Fragments arranged by size:
1800 1241 1041 721 281
AhdI GACnn_n'nnGTC
Cuts at: 501 501
Size: 5084
AlwNI CAG_nnn'CTG
Cuts at: 128 1157 1281 1816 2389 3827 128
Size: 1029 124 535 573 1438 1385
Fragments arranged by size:
1438 1385 1029 573 535 124
ApaI G_GGCC'C
Cuts at: 248 351 521 545 1763 1776 248
Size: 103 170 24 1218 13 3556
Fragments arranged by size:
3556 1218 170 103 24 13
ApaLI G'TGCA_C
Cuts at: 3922 3922
Size: 5084
ApoI r'AATT_y
Cuts at: 2088 4437 4693 2088
Size: 2349 256 2479
Fragments arranged by size:
2479 2349 256
AscI GG'CGCG_CC
Cuts at: 1728 1728
Size: 5084
AseI AT'TA_AT
Cuts at: 20 20
Size: 5084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
AvaI C'yCGr_G
Cuts at: 168 266 363 398 538 682 790 1056 1890
Size: 98 97 35 140 144 108 266 834
Cuts at: 1890 1924 2104 168
Size: 34 180 3148
Fragments arranged by size:
3148 834 266 180 144 140 108 98
97 35 34
BamHI G'GATC_C
Cuts at: 172 765 832 2109 172
Size: 593 67 1277 3147
Fragments arranged by size:
3147 1277 593 67
BanI G'GyrC_C
Cuts at: 450 696 970 1040 1171 1894 2100 2514 2762
Size: 246 274 70 131 723 206 414 248
Cuts at: 2762 2797 450
Size: 35 2737
Fragments arranged by size:
2737 723 414 274 248 246 206 131
70 35
BanII G_rGCy'C
Cuts at: 135 248 267 351 361 386 422 521 545
Size: 113 19 84 10 25 36 99 24
Cuts at: 545 872 1057 1164 1598 1686 1763 1776 1847
Size: 327 185 107 434 88 77 13 71
Cuts at: 1847 2098 3128 135
Size: 251 1030 2091
Fragments arranged by size:
2091 1030 434 327 251 185 113 107
99 88 84 77 71 36 25 24
19 13 10
BbeI G_GCGC'C
Cuts at: 454 1898 2518 2766 454
Size: 1444 620 248 2772
Fragments arranged by size:
2772 1444 620 248
BbsI GAAGACnn'nnnn_
Cuts at: 1966 1966
Size: 5084
BbvCI CC'TCA_GC
Cuts at: 1538 1838 1538
Size: 300 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 864 898 864
Size: 34 5050
BciVI GTATCCnnnnn_n'
Cuts at: 1807 2982 4038 5084 1807
Size: 1175 1056 1046 1807
Fragments arranged by size:
1807 1175 1056 1046
BclI T'GATC_A
Cuts at: 2604 2604
Size: 5084
BglI GCCn_nnn'nGGC
Cuts at: 195 412 459 1529 195
Size: 217 47 1070 3750
Fragments arranged by size:
3750 1070 217 47
BglII A'GATC_T
Cuts at: 1082 2599 4636 1082
Size: 1517 2037 1530
Fragments arranged by size:
2037 1530 1517
BlpI GC'TnA_GC
Cuts at: 1846 2196 1846
Size: 350 4734
Bme1580I G_kGCm'C
Cuts at: 248 351 521 545 701 1017 1254 1763 1776
Size: 103 170 24 156 316 237 509 13
Cuts at: 1776 2709 2802 3926 248
Size: 933 93 1124 1406
Fragments arranged by size:
1406 1124 933 509 316 237 170 156
103 93 24 13
BmrI ACTGGGnnnn_n'
Cuts at: 138 2296 2377 2712 4856 138
Size: 2158 81 335 2144 366
Fragments arranged by size:
2158 2144 366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1205 3451 1205
Size: 2246 2838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 710 1246 1588 192
Size: 518 536 342 3688
Fragments arranged by size:
3688 536 518 342
Bpu10I CC'TnA_GC
Cuts at: 1538 1838 1538
Size: 300 4784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 2260 3139 3853 4151 1033
Size: 1227 879 714 298 1966
Fragments arranged by size:
1966 1227 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 310 808 826 1213 4788 310
Size: 498 18 387 3575 606
Fragments arranged by size:
3575 606 498 387 18
BsaAI yAC'GTr
Cuts at: 155 627 1319 2437 155
Size: 472 692 1118 2802
Fragments arranged by size:
2802 1118 692 472
BsaBI GATnn'nnATC
Cuts at: 6 2598 4635 6
Size: 2592 2037 455
Fragments arranged by size:
2592 2037 455
BsaHI Gr'CG_yC
Cuts at: 292 451 600 785 1298 1313 1625 1895 2515
Size: 159 149 185 513 15 312 270 620
Cuts at: 2515 2763 3465 4961 292
Size: 248 702 1496 415
Fragments arranged by size:
1496 702 620 513 415 312 270 248
185 159 149 15
BsaWI w'CCGG_w
Cuts at: 1174 1215 2273 2794 3472 3883 4030 4304 1174
Size: 41 1058 521 678 411 147 274 1954
Fragments arranged by size:
1954 1058 678 521 411 274 147 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 990 1020 990
Size: 30 5054
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1453 1585 1854 1855 2032 1453
Size: 132 269 1 177 4505
Fragments arranged by size:
4505 269 177 132 1
BseYI C'CCAG_C
Cuts at: 407 605 714 1361 1414 1668 1742 2509 3267
Size: 198 109 647 53 254 74 767 758
Cuts at: 3267 3932 4340 407
Size: 665 408 1151
Fragments arranged by size:
1151 767 758 665 647 408 254 198
109 74 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 1390
Size: 5084
BsiEI CG_ry'CG
Cuts at: 104 439 616 1737 2626 2672 3902 104
Size: 335 177 1121 889 46 1230 1286
Fragments arranged by size:
1286 1230 1121 889 335 177 46
BsiHKAI G_wGCw'C
Cuts at: 267 1655 1847 2098 2876 3066 3926 267
Size: 1388 192 251 778 190 860 1425
Fragments arranged by size:
1425 1388 860 778 251 192 190
Bsp1286I G_dGCh'C
Cuts at: 135 248 267 351 361 386 422 521 545
Size: 113 19 84 10 25 36 99 24
Cuts at: 545 701 872 1017 1057 1164 1254 1598 1655
Size: 156 171 145 40 107 90 344 57
Cuts at: 1655 1686 1763 1776 1847 2098 2709 2802 2876
Size: 31 77 13 71 251 611 93 74
Cuts at: 2876 3066 3128 3926 135
Size: 190 62 798 1293
Fragments arranged by size:
1293 798 611 344 251 190 171 156
145 113 107 99 93 90 84 77
74 71 62 57 40 36 31 25
24 19 13 10
BspHI T'CATG_A
Cuts at: 3516 3516
Size: 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 572 1356 1625 2067 2134 2348 2650 3031 572
Size: 784 269 442 67 214 302 381 2625
Fragments arranged by size:
2625 784 442 381 302 269 214 67
BsrBI CCG'CTC
Cuts at: 50 303 850 1027 3376 3430 4914 5079 50
Size: 253 547 177 2349 54 1484 165 55
Fragments arranged by size:
2349 1484 547 253 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2996 2996
Size: 5084
BsrFI r'CCGG_y
Cuts at: 194 611 1732 3082 3263 194
Size: 417 1121 1350 181 2015
Fragments arranged by size:
2015 1350 1121 417 181
BssHII G'CGCG_C
Cuts at: 1728 1728
Size: 5084
BssSI C'ACGA_G
Cuts at: 3355 4063 3355
Size: 708 4376
BstAPI GCAn_nnn'nTGC
Cuts at: 1377 2461 1377
Size: 1084 4000
BstBI TT'CG_AA
Cuts at: 3445 3445
Size: 5084
BstYI r'GATC_y
Cuts at: 1 172 765 773 832 1033 1082 1991 2109
Size: 171 593 8 59 201 49 909 118
Cuts at: 2109 2576 2599 2934 3180 3486 3498 3584 4636
Size: 467 23 335 246 306 12 86 1052
Cuts at: 4636 1
Size: 449
Fragments arranged by size:
1052 909 593 467 449 335 306 246
201 171 118 86 59 49 23 12
8
Bsu36I CC'TnA_GG
Cuts at: 1662 1662
Size: 5084
BtgI C'CryG_G
Cuts at: 107 1287 1454 1524 107
Size: 1180 167 70 3667
Fragments arranged by size:
3667 1180 167 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3034 3215 3378 3034
Size: 181 163 4740
Fragments arranged by size:
4740 181 163
BtsI GCAGTG_nn'
Cuts at: 2327 2327
Size: 5084
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 324 359 324
Size: 35 5049
DraI TTT'AAA
Cuts at: 2083 2083
Size: 5084
DrdI GACnn_nn'nnGTC
Cuts at: 2790 4134 2790
Size: 1344 3740
EaeI y'GGCC_r
Cuts at: 251 436 613 851 1734 2669 2843 3234 3261
Size: 185 177 238 883 935 174 391 27
Cuts at: 3261 4606 251
Size: 1345 729
Fragments arranged by size:
1345 935 883 729 391 238 185 177
174 27
EagI C'GGCC_G
Cuts at: 436 613 1734 2669 436
Size: 177 1121 935 2851
Fragments arranged by size:
2851 1121 935 177
EarI CTCTTCn'nnn_
Cuts at: 751 2032 2065 3107 3317 751
Size: 1281 33 1042 210 2518
Fragments arranged by size:
2518 1281 1042 210 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1789 1920 3292 4034 4180 4894 1789
Size: 131 1372 742 146 714 1979
Fragments arranged by size:
1979 1372 742 714 146 131
EcoICRI GAG'CTC
Cuts at: 265 1845 2096 265
Size: 1580 251 3253
Fragments arranged by size:
3253 1580 251
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 710 1246 1588 2909 3341 3694 4356 192
Size: 518 536 342 1321 432 353 662 920
Fragments arranged by size:
1321 920 662 536 518 432 353 342
EcoNI CCTnn'n_nnAGG
Cuts at: 253 1924 253
Size: 1671 3413
EcoO109I rG'GnC_Cy
Cuts at: 244 245 347 517 518 542 649 659 679
Size: 1 102 170 1 24 107 10 20
Cuts at: 679 1278 1759 1760 1772 1773 1916 2022 2051
Size: 599 481 1 12 1 143 106 29
Cuts at: 2051 2223 244
Size: 172 3105
Fragments arranged by size:
3105 599 481 172 170 143 107 106
102 29 24 20 12 10 1 1
1 1
EcoRI G'AATT_C
Cuts at: 2088 2088
Size: 5084
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3103 3103
Size: 5084
FspI TGC'GCA
Cuts at: 2865 2865
Size: 5084
HaeII r_GCGC'y
Cuts at: 447 454 469 1898 2518 2766 3996 4767 447
Size: 7 15 1429 620 248 1230 771 764
Fragments arranged by size:
1429 1230 771 764 620 248 15 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 750 782 1067 1099 2926 2956 2958
Size: 32 231 32 285 32 1827 30 2
Cuts at: 2958 2988 487
Size: 30 2583
Fragments arranged by size:
2583 1827 285 231 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 370 2123 370
Size: 1753 3331
KasI G'GCGC_C
Cuts at: 450 1894 2514 2762 450
Size: 1444 620 248 2772
Fragments arranged by size:
2772 1444 620 248
KpnI G_GTAC'C
Cuts at: 2104 2104
Size: 5084
MluI A'CGCG_T
Cuts at: 1395 4517 1395
Size: 3122 1962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 831 1213 1372 1685 1911 3843 4027 831
Size: 382 159 313 226 1932 184 1888
Fragments arranged by size:
1932 1888 382 313 226 184 159
MscI TGG'CCA
Cuts at: 2845 2845
Size: 5084
MslI CAynn'nnrTG
Cuts at: 3073 3200 3073
Size: 127 4957
MspA1I CmG'CkG
Cuts at: 411 605 718 1526 1725 1966 2195 2509 2869
Size: 194 113 808 199 241 229 314 360
Cuts at: 2869 3653 3898 411
Size: 784 245 1597
Fragments arranged by size:
1597 808 784 360 314 245 241 229
199 194 113
NaeI GCC'GGC
Cuts at: 196 1734 3265 196
Size: 1538 1531 2015
Fragments arranged by size:
2015 1538 1531
NarI GG'CG_CC
Cuts at: 451 1895 2515 2763 451
Size: 1444 620 248 2772
Fragments arranged by size:
2772 1444 620 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5084
NgoMIV G'CCGG_C
Cuts at: 194 1732 3263 194
Size: 1538 1531 2015
Fragments arranged by size:
2015 1538 1531
NspI r_CATG'y
Cuts at: 2137 2637 4240 2137
Size: 500 1603 2981
Fragments arranged by size:
2981 1603 500
PasI CC'CwG_GG
Cuts at: 200 499 521 1157 1275 1686 1756 200
Size: 299 22 636 118 411 70 3528
Fragments arranged by size:
3528 636 411 299 118 70 22
PciI A'CATG_T
Cuts at: 4236 4236
Size: 5084
PflMI CCAn_nnn'nTGG
Cuts at: 128 844 1100 1157 128
Size: 716 256 57 4055
Fragments arranged by size:
4055 716 256 57
PfoI T'CCnGG_A
Cuts at: 1164 1164
Size: 5084
PmeI GTTT'AAAC
Cuts at: 2083 2083
Size: 5084
PmlI CAC'GTG
Cuts at: 155 1319 155
Size: 1164 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 3530
Size: 32 5052
PpuMI rG'GwC_Cy
Cuts at: 659 1916 2022 2051 659
Size: 1257 106 29 3692
Fragments arranged by size:
3692 1257 106 29
PspOMI G'GGCC_C
Cuts at: 244 347 517 541 1759 1772 244
Size: 103 170 24 1218 13 3556
Fragments arranged by size:
3556 1218 170 103 24 13
PspXI vC'TCGA_Gb
Cuts at: 1056 1056
Size: 5084
PstI C_TGCA'G
Cuts at: 221 2131 2816 221
Size: 1910 685 2489
Fragments arranged by size:
2489 1910 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5084
PvuII CAG'CTG
Cuts at: 411 718 2509 2869 411
Size: 307 1791 360 2626
Fragments arranged by size:
2626 1791 360 307
RsrII CG'GwC_CG
Cuts at: 3279 3279
Size: 5084
SacI G_AGCT'C
Cuts at: 267 1847 2098 267
Size: 1580 251 3253
Fragments arranged by size:
3253 1580 251
SacII CC_GC'GG
Cuts at: 1527 1527
Size: 5084
SalI G'TCGA_C
Cuts at: 368 2121 368
Size: 1753 3331
SanDI GG'GwC_CC
Cuts at: 2051 2051
Size: 5084
SapI GCTCTTCn'nnn_
Cuts at: 3107 3317 3107
Size: 210 4874
SbfI CC_TGCA'GG
Cuts at: 2131 2131
Size: 5084
ScaI AGT'ACT
Cuts at: 4601 4601
Size: 5084
SfcI C'TryA_G
Cuts at: 33 217 956 2127 2812 3780 3971 33
Size: 184 739 1171 685 968 191 1146
Fragments arranged by size:
1171 1146 968 739 685 191 184
SfoI GGC'GCC
Cuts at: 452 1896 2516 2764 452
Size: 1444 620 248 2772
Fragments arranged by size:
2772 1444 620 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
SmaI CCC'GGG
Cuts at: 170 365 540 684 1892 2106 170
Size: 195 175 144 1208 214 3148
Fragments arranged by size:
3148 1208 214 195 175 144
SmlI C'TyrA_G
Cuts at: 1048 1056 2239 3154 3868 4130 1048
Size: 8 1183 915 714 262 2002
Fragments arranged by size:
2002 1183 915 714 262 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5084
SphI G_CATG'C
Cuts at: 2137 2637 2137
Size: 500 4584
SrfI GCCC'GGGC
Cuts at: 540 540
Size: 5084
StyI C'CwwG_G
Cuts at: 107 311 662 2218 2572 107
Size: 204 351 1556 354 2619
Fragments arranged by size:
2619 1556 354 351 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 2667 2800 3259 3468 334
Size: 2333 133 459 209 1950
Fragments arranged by size:
2333 1950 459 209 133
TatI w'GTAC_w
Cuts at: 4599 4599
Size: 5084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1446 1478 4601 4633 1446
Size: 32 3123 32 1897
Fragments arranged by size:
3123 1897 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 2881 1981
Size: 900 4184
XbaI T'CTAG_A
Cuts at: 2115 2115
Size: 5084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 506 602 2579 506
Size: 96 1977 3011
Fragments arranged by size:
3011 1977 96
XhoI C'TCGA_G
Cuts at: 1056 1056
Size: 5084
XmaI C'CCGG_G
Cuts at: 168 363 538 682 1890 2104 168
Size: 195 175 144 1208 214 3148
Fragments arranged by size:
3148 1208 214 195 175 144
XmnI GAAnn'nnTTC
Cuts at: 1938 1938
Size: 5084
ZraI GAC'GTC
Cuts at: 786 786
Size: 5084
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AhdI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AgeI AleI AlfI AloI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BspEI BsrGI
BstEII BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PsrI SexAI SfiI SgrAI SnaBI
SspI StuI SwaI