Restriction map for
pF1KA0737
(Circular) (Six-base) MAPSORT of: pF1KA0737.seq Check: 884 from: 1 to: 4976
pF1KA0737 4976 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 609 609
Size: 4976
AccI GT'mk_AC
Cuts at: 2014 2014
Size: 4976
Acc65I G'GTAC_C
Cuts at: 1992 1992
Size: 4976
AclI AA'CG_TT
Cuts at: 4816 4816
Size: 4976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 979 2801 3233 3980 4248 635
Size: 344 1822 432 747 268 1363
Fragments arranged by size:
1822 1363 747 432 344 268
AflIII A'CryG_T
Cuts at: 2328 3432 4409 2328
Size: 1104 977 2895
Fragments arranged by size:
2895 1104 977
AgeI A'CCGG_T
Cuts at: 3364 3364
Size: 4976
AleI CACnn'nnGTG
Cuts at: 1288 1957 1288
Size: 669 4307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 610 610
Size: 4976
AlwNI CAG_nnn'CTG
Cuts at: 1393 1396 2281 3848 1393
Size: 3 885 1567 2521
Fragments arranged by size:
2521 1567 885 3
ApaLI G'TGCA_C
Cuts at: 3746 3746
Size: 4976
ApoI r'AATT_y
Cuts at: 214 1944 1980 4329 4585 214
Size: 1730 36 2349 256 605
Fragments arranged by size:
2349 1730 605 256 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 4976
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
AvaI C'yCGr_G
Cuts at: 1298 1996 1298
Size: 698 4278
AvrII C'CTAG_G
Cuts at: 576 576
Size: 4976
BamHI G'GATC_C
Cuts at: 1023 2001 1023
Size: 978 3998
BanI G'GyrC_C
Cuts at: 586 1698 1992 2406 2654 2689 586
Size: 1112 294 414 248 35 2873
Fragments arranged by size:
2873 1112 414 294 248 35
BanII G_rGCy'C
Cuts at: 404 1109 1307 1990 3020 404
Size: 705 198 683 1030 2360
Fragments arranged by size:
2360 1030 705 683 198
BbeI G_GCGC'C
Cuts at: 2410 2658 2410
Size: 248 4728
BbsI GAAGACnn'nnnn_
Cuts at: 709 709
Size: 4976
BciVI GTATCCnnnnn_n'
Cuts at: 2874 3635 4976 2874
Size: 761 1341 2874
Fragments arranged by size:
2874 1341 761
BclI T'GATC_A
Cuts at: 540 1727 2496 540
Size: 1187 769 3020
Fragments arranged by size:
3020 1187 769
BglII A'GATC_T
Cuts at: 2491 4528 2491
Size: 2037 2939
BlpI GC'TnA_GC
Cuts at: 2088 2088
Size: 4976
Bme1580I G_kGCm'C
Cuts at: 2601 2694 3750 2601
Size: 93 1056 3827
Fragments arranged by size:
3827 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 327 600 1337 1875 2188 2269 2604 4748 327
Size: 273 737 538 313 81 335 2144 555
Fragments arranged by size:
2144 737 555 538 335 313 273 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1613 1808 3343 1613
Size: 195 1535 3246
Fragments arranged by size:
3246 1535 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1199 1498 1070
Size: 129 299 4548
Fragments arranged by size:
4548 299 129
Bpu10I CC'TnA_GC
Cuts at: 1655 1655
Size: 4976
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1238 2152 3031 3523 3821 1238
Size: 914 879 492 298 2393
Fragments arranged by size:
2393 914 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1796 4680 1796
Size: 2884 2092
BsaAI yAC'GTr
Cuts at: 2329 2329
Size: 4976
BsaBI GATnn'nnATC
Cuts at: 6 245 2490 4527 6
Size: 239 2245 2037 455
Fragments arranged by size:
2245 2037 455 239
BsaHI Gr'CG_yC
Cuts at: 2407 2655 3357 4853 2407
Size: 248 702 1496 2530
Fragments arranged by size:
2530 1496 702 248
BsaWI w'CCGG_w
Cuts at: 680 2165 2686 3364 3638 3785 4196 680
Size: 1485 521 678 274 147 411 1460
Fragments arranged by size:
1485 1460 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1035 1065 1250 1280 1035
Size: 30 185 30 4731
Fragments arranged by size:
4731 185 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 919 1496 1562 1565 919
Size: 577 66 3 4330
Fragments arranged by size:
4330 577 66 3
BseYI C'CCAG_C
Cuts at: 560 564 694 1077 1183 1278 1811 2401 3159
Size: 4 130 383 106 95 533 590 758
Cuts at: 3159 3736 4232 560
Size: 577 496 1304
Fragments arranged by size:
1304 758 590 577 533 496 383 130
106 95 4
BsiEI CG_ry'CG
Cuts at: 104 2518 2564 3772 104
Size: 2414 46 1208 1308
Fragments arranged by size:
2414 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1990 2768 2958 3750 1990
Size: 778 190 792 3216
Fragments arranged by size:
3216 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 404 1109 1307 1990 2601 2694 2768 2958 3020
Size: 705 198 683 611 93 74 190 62
Cuts at: 3020 3750 404
Size: 730 1630
Fragments arranged by size:
1630 730 705 683 611 198 190 93
74 62
BspEI T'CCGG_A
Cuts at: 680 4196 680
Size: 3516 1460
BspHI T'CATG_A
Cuts at: 4152 4152
Size: 4976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 609 2026 2240 2542 2923 609
Size: 1417 214 302 381 2662
Fragments arranged by size:
2662 1417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1659 3268 3322 4806 4971 50
Size: 1609 1609 54 1484 165 55
Fragments arranged by size:
1609 1609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1893 2888 1893
Size: 995 3981
BsrFI r'CCGG_y
Cuts at: 2974 3155 3364 2974
Size: 181 209 4586
Fragments arranged by size:
4586 209 181
BssSI C'ACGA_G
Cuts at: 658 3247 3605 658
Size: 2589 358 2029
Fragments arranged by size:
2589 2029 358
BstAPI GCAn_nnn'nTGC
Cuts at: 970 1393 1396 2353 970
Size: 423 3 957 3593
Fragments arranged by size:
3593 957 423 3
BstBI TT'CG_AA
Cuts at: 1548 3337 1548
Size: 1789 3187
BstEII G'GTnAC_C
Cuts at: 1290 1290
Size: 4976
BstXI CCAn_nnnn'nTGG
Cuts at: 429 429
Size: 4976
BstYI r'GATC_y
Cuts at: 1 762 1023 2001 2468 2491 2826 3072 4084
Size: 761 261 978 467 23 335 246 1012
Cuts at: 4084 4170 4182 4528 1
Size: 86 12 346 449
Fragments arranged by size:
1012 978 761 467 449 346 335 261
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1679 1745 1679
Size: 66 4910
BtgI C'CryG_G
Cuts at: 107 375 422 107
Size: 268 47 4661
Fragments arranged by size:
4661 268 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2926 3107 3270 2926
Size: 181 163 4632
Fragments arranged by size:
4632 181 163
BtsI GCAGTG_nn'
Cuts at: 1905 2219 1905
Size: 314 4662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 857 892 1937 1972 857
Size: 35 1045 35 3861
Fragments arranged by size:
3861 1045 35 35
DraI TTT'AAA
Cuts at: 1975 1975
Size: 4976
DrdI GACnn_nn'nnGTC
Cuts at: 2682 3540 2682
Size: 858 4118
EaeI y'GGCC_r
Cuts at: 276 2561 2735 3126 3153 4498 276
Size: 2285 174 391 27 1345 754
Fragments arranged by size:
2285 1345 754 391 174 27
EagI C'GGCC_G
Cuts at: 2561 2561
Size: 4976
EarI CTCTTCn'nnn_
Cuts at: 312 742 1242 2999 3209 312
Size: 430 500 1757 210 2079
Fragments arranged by size:
2079 1757 500 430 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3184 3494 3640 4786 3184
Size: 310 146 1146 3374
Fragments arranged by size:
3374 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1988 1988
Size: 4976
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 979 1070 1199 1498 2801 3233 3980 4248
Size: 344 91 129 299 1303 432 747 268
Cuts at: 4248 635
Size: 1363
Fragments arranged by size:
1363 1303 747 432 344 299 268 129
91
EcoO109I rG'GnC_Cy
Cuts at: 151 738 2115 151
Size: 587 1377 3012
Fragments arranged by size:
3012 1377 587
EcoRI G'AATT_C
Cuts at: 1980 1980
Size: 4976
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 640 2995 640
Size: 2355 2621
FspI TGC'GCA
Cuts at: 2757 2757
Size: 4976
HaeII r_GCGC'y
Cuts at: 2410 2658 3680 4659 2410
Size: 248 1022 979 2727
Fragments arranged by size:
2727 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 487 519 1066 1098 1350 1382 1738
Size: 32 166 32 547 32 252 32 356
Cuts at: 1738 1770 2818 2848 2850 2880 289
Size: 32 1048 30 2 30 2385
Fragments arranged by size:
2385 1048 547 356 252 166 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 530 1074 1137 1348 2015 530
Size: 544 63 211 667 3491
Fragments arranged by size:
3491 667 544 211 63
HindIII A'AGCT_T
Cuts at: 198 198
Size: 4976
HpaI GTT'AAC
Cuts at: 1137 1137
Size: 4976
KasI G'GCGC_C
Cuts at: 2406 2654 2406
Size: 248 4728
KpnI G_GTAC'C
Cuts at: 1996 1996
Size: 4976
MfeI C'AATT_G
Cuts at: 476 476
Size: 4976
MluI A'CGCG_T
Cuts at: 4409 4409
Size: 4976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1332 3647 3831 1332
Size: 2315 184 2477
Fragments arranged by size:
2477 2315 184
MscI TGG'CCA
Cuts at: 278 2737 278
Size: 2459 2517
MslI CAynn'nnrTG
Cuts at: 284 361 662 1288 1957 2965 3092 284
Size: 77 301 626 669 1008 127 2168
Fragments arranged by size:
2168 1008 669 626 301 127 77
MspA1I CmG'CkG
Cuts at: 564 1393 2087 2401 2761 3774 4019 564
Size: 829 694 314 360 1013 245 1521
Fragments arranged by size:
1521 1013 829 694 360 314 245
NaeI GCC'GGC
Cuts at: 3157 3157
Size: 4976
NarI GG'CG_CC
Cuts at: 2407 2655 2407
Size: 248 4728
NcoI C'CATG_G
Cuts at: 107 375 422 107
Size: 268 47 4661
Fragments arranged by size:
4661 268 47
NdeI CA'TA_TG
Cuts at: 795 795
Size: 4976
NgoMIV G'CCGG_C
Cuts at: 3155 3155
Size: 4976
NspI r_CATG'y
Cuts at: 360 2029 2529 3436 360
Size: 1669 500 907 1900
Fragments arranged by size:
1900 1669 907 500
PciI A'CATG_T
Cuts at: 3432 3432
Size: 4976
PflMI CCAn_nnn'nTGG
Cuts at: 351 1692 351
Size: 1341 3635
PfoI T'CCnGG_A
Cuts at: 117 117
Size: 4976
PmeI GTTT'AAAC
Cuts at: 1975 1975
Size: 4976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 4115
Size: 32 4944
PstI C_TGCA'G
Cuts at: 1499 1583 1885 1912 2023 2708 1499
Size: 84 302 27 111 685 3767
Fragments arranged by size:
3767 685 302 111 84 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4976
PvuII CAG'CTG
Cuts at: 564 1393 2401 2761 564
Size: 829 1008 360 2779
Fragments arranged by size:
2779 1008 829 360
RsrII CG'GwC_CG
Cuts at: 3171 3171
Size: 4976
SacI G_AGCT'C
Cuts at: 1990 1990
Size: 4976
SalI G'TCGA_C
Cuts at: 2013 2013
Size: 4976
SapI GCTCTTCn'nnn_
Cuts at: 2999 3209 2999
Size: 210 4766
SbfI CC_TGCA'GG
Cuts at: 2023 2023
Size: 4976
ScaI AGT'ACT
Cuts at: 4493 4493
Size: 4976
SfcI C'TryA_G
Cuts at: 33 440 1435 1495 1579 1881 1908 2019 2704
Size: 407 995 60 84 302 27 111 685
Cuts at: 2704 3697 3888 33
Size: 993 191 1121
Fragments arranged by size:
1121 995 993 685 407 302 191 111
84 60 27
SfoI GGC'GCC
Cuts at: 2408 2656 2408
Size: 248 4728
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4976
SmaI CCC'GGG
Cuts at: 1998 1998
Size: 4976
SmlI C'TyrA_G
Cuts at: 1253 2131 3046 3538 3800 1253
Size: 878 915 492 262 2429
Fragments arranged by size:
2429 915 878 492 262
SpeI A'CTAG_T
Cuts at: 67 646 67
Size: 579 4397
SphI G_CATG'C
Cuts at: 2029 2529 2029
Size: 500 4476
StyI C'CwwG_G
Cuts at: 107 236 375 422 576 2110 2464 107
Size: 129 139 47 154 1534 354 2619
Fragments arranged by size:
2619 1534 354 154 139 129 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 1358 2559 2692 3151 3360 193
Size: 1165 1201 133 459 209 1809
Fragments arranged by size:
1809 1201 1165 459 209 133
TatI w'GTAC_w
Cuts at: 1324 4491 1324
Size: 3167 1809
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 4944
Tth111I GACn'n_nGTC
Cuts at: 2773 2773
Size: 4976
XbaI T'CTAG_A
Cuts at: 1939 2007 1939
Size: 68 4908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1174 2471 1174
Size: 1297 3679
XmaI C'CCGG_G
Cuts at: 1996 1996
Size: 4976
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI