Restriction map for
pF1KA0727
(Circular) (Six-base) MAPSORT of: pF1KA0727.seq Check: 9509 from: 1 to: 6131
pF1KA0727 6131 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1835 3169 1835
Size: 1334 4797
Acc65I G'GTAC_C
Cuts at: 3147 3147
Size: 6131
AclI AA'CG_TT
Cuts at: 3125 5971 3125
Size: 2846 3285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 1772 2051 2886 3956 4388 5135 5403 1375
Size: 397 279 835 1070 432 747 268 2103
Fragments arranged by size:
2103 1070 835 747 432 397 279 268
AflII C'TTAA_G
Cuts at: 523 523
Size: 6131
AflIII A'CryG_T
Cuts at: 3483 4587 5564 3483
Size: 1104 977 4050
Fragments arranged by size:
4050 1104 977
AgeI A'CCGG_T
Cuts at: 4519 4519
Size: 6131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 479 479
Size: 6131
AlwNI CAG_nnn'CTG
Cuts at: 3436 5003 3436
Size: 1567 4564
ApaLI G'TGCA_C
Cuts at: 3036 4901 3036
Size: 1865 4266
ApoI r'AATT_y
Cuts at: 130 844 942 949 1592 1619 1815 2038 2141
Size: 714 98 7 643 27 196 223 103
Cuts at: 2141 3135 5484 5740 130
Size: 994 2349 256 521
Fragments arranged by size:
2349 994 714 643 521 256 223 196
103 98 27 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 6131
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6131
AvaI C'yCGr_G
Cuts at: 175 674 2557 3096 3118 3151 175
Size: 499 1883 539 22 33 3155
Fragments arranged by size:
3155 1883 539 499 33 22
BamHI G'GATC_C
Cuts at: 1388 2769 3156 1388
Size: 1381 387 4363
Fragments arranged by size:
4363 1381 387
BanI G'GyrC_C
Cuts at: 1928 2239 3147 3561 3809 3844 1928
Size: 311 908 414 248 35 4215
Fragments arranged by size:
4215 908 414 311 248 35
BanII G_rGCy'C
Cuts at: 813 2564 3145 4175 813
Size: 1751 581 1030 2769
Fragments arranged by size:
2769 1751 1030 581
BbeI G_GCGC'C
Cuts at: 3565 3813 3565
Size: 248 5883
BbsI GAAGACnn'nnnn_
Cuts at: 815 2140 2732 2803 815
Size: 1325 592 71 4143
Fragments arranged by size:
4143 1325 592 71
BbvCI CC'TCA_GC
Cuts at: 3109 3109
Size: 6131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1476 1510 1476
Size: 34 6097
BciVI GTATCCnnnnn_n'
Cuts at: 4029 4790 6131 4029
Size: 761 1341 4029
Fragments arranged by size:
4029 1341 761
BclI T'GATC_A
Cuts at: 3651 3651
Size: 6131
BglII A'GATC_T
Cuts at: 3646 5683 3646
Size: 2037 4094
BlpI GC'TnA_GC
Cuts at: 3243 3243
Size: 6131
Bme1580I G_kGCm'C
Cuts at: 2193 2242 3040 3119 3756 3849 4905 2193
Size: 49 798 79 637 93 1056 3419
Fragments arranged by size:
3419 1056 798 637 93 79 49
BmgBI CAC'GTC
Cuts at: 2694 2694
Size: 6131
BmrI ACTGGGnnnn_n'
Cuts at: 476 3001 3343 3424 3759 5903 476
Size: 2525 342 81 335 2144 704
Fragments arranged by size:
2525 2144 704 342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 350 4498 350
Size: 4148 1983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1618 2548 754
Size: 864 930 4337
Fragments arranged by size:
4337 930 864
Bpu10I CC'TnA_GC
Cuts at: 3109 3109
Size: 6131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 1720 3307 4186 4678 4976 567
Size: 1153 1587 879 492 298 1722
Fragments arranged by size:
1722 1587 1153 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 321 5835 321
Size: 5514 617
BsaAI yAC'GTr
Cuts at: 2385 3484 2385
Size: 1099 5032
BsaBI GATnn'nnATC
Cuts at: 6 3645 5682 6
Size: 3639 2037 455
Fragments arranged by size:
3639 2037 455
BsaHI Gr'CG_yC
Cuts at: 1130 2354 3562 3810 4512 6008 1130
Size: 1224 1208 248 702 1496 1253
Fragments arranged by size:
1496 1253 1224 1208 702 248
BsaWI w'CCGG_w
Cuts at: 1058 3320 3841 4519 4793 4940 5351 1058
Size: 2262 521 678 274 147 411 1838
Fragments arranged by size:
2262 1838 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 151 181 227 257 2927 2939 2957 2969 151
Size: 30 46 30 2670 12 18 12 3313
Fragments arranged by size:
3313 2670 46 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 907 2440 907
Size: 1533 4598
BseYI C'CCAG_C
Cuts at: 2465 3071 3556 4314 4891 5387 2465
Size: 606 485 758 577 496 3209
Fragments arranged by size:
3209 758 606 577 496 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 3007 3036 916
Size: 2091 29 4011
Fragments arranged by size:
4011 2091 29
BsiEI CG_ry'CG
Cuts at: 104 3673 3719 4927 104
Size: 3569 46 1208 1308
Fragments arranged by size:
3569 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 813 1736 3040 3145 3923 4113 4905 813
Size: 923 1304 105 778 190 792 2039
Fragments arranged by size:
2039 1304 923 792 778 190 105
BsmI GAATG_Cn'
Cuts at: 2480 2480
Size: 6131
BsmBI CGTCTCn'nnnn_
Cuts at: 171 1121 3046 3048 171
Size: 950 1925 2 3254
Fragments arranged by size:
3254 1925 950 2
Bsp1286I G_dGCh'C
Cuts at: 813 1736 2193 2242 2564 3040 3119 3145 3756
Size: 923 457 49 322 476 79 26 611
Cuts at: 3756 3849 3923 4113 4175 4905 813
Size: 93 74 190 62 730 2039
Fragments arranged by size:
2039 923 730 611 476 457 322 190
93 79 74 62 49 26
BspEI T'CCGG_A
Cuts at: 5351 5351
Size: 6131
BspHI T'CATG_A
Cuts at: 5307 5307
Size: 6131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 2504 3181 3395 3697 4078 578
Size: 1926 677 214 302 381 2631
Fragments arranged by size:
2631 1926 677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4423 4477 5961 6126 50
Size: 4373 54 1484 165 55
Fragments arranged by size:
4373 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1323 1463 4043 1323
Size: 140 2580 3411
Fragments arranged by size:
3411 2580 140
BsrFI r'CCGG_y
Cuts at: 1925 2300 4129 4310 4519 1925
Size: 375 1829 181 209 3537
Fragments arranged by size:
3537 1829 375 209 181
BssSI C'ACGA_G
Cuts at: 1438 2329 4402 4760 1438
Size: 891 2073 358 2809
Fragments arranged by size:
2809 2073 891 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1984 2518 3508 1984
Size: 534 990 4607
Fragments arranged by size:
4607 990 534
BstBI TT'CG_AA
Cuts at: 1617 2151 4492 1617
Size: 534 2341 3256
Fragments arranged by size:
3256 2341 534
BstEII G'GTnAC_C
Cuts at: 628 1776 628
Size: 1148 4983
BstYI r'GATC_y
Cuts at: 1 896 1388 2769 3156 3623 3646 3981 4227
Size: 895 492 1381 387 467 23 335 246
Cuts at: 4227 5239 5325 5337 5683 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1381 1012 895 492 467 449 387 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 193 890 2619 193
Size: 697 1729 3705
Fragments arranged by size:
3705 1729 697
BtgI C'CryG_G
Cuts at: 107 1224 2349 2525 3047 107
Size: 1117 1125 176 522 3191
Fragments arranged by size:
3191 1125 1117 522 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1652 4081 4262 4425 1652
Size: 2429 181 163 3358
Fragments arranged by size:
3358 2429 181 163
BtsI GCAGTG_nn'
Cuts at: 3374 3374
Size: 6131
ClaI AT'CG_AT
Cuts at: 2709 2709
Size: 6131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2212 2247 2212
Size: 35 6096
DraI TTT'AAA
Cuts at: 947 3130 947
Size: 2183 3948
DraIII CAC_nnn'GTG
Cuts at: 2334 2334
Size: 6131
DrdI GACnn_nn'nnGTC
Cuts at: 2890 3837 4695 2890
Size: 947 858 4326
Fragments arranged by size:
4326 947 858
EaeI y'GGCC_r
Cuts at: 185 467 1067 2336 2392 3716 3890 4281 4308
Size: 282 600 1269 56 1324 174 391 27
Cuts at: 4308 5653 185
Size: 1345 663
Fragments arranged by size:
1345 1324 1269 663 600 391 282 174
56 27
EagI C'GGCC_G
Cuts at: 3716 3716
Size: 6131
EarI CTCTTCn'nnn_
Cuts at: 279 370 2905 4154 4364 279
Size: 91 2535 1249 210 2046
Fragments arranged by size:
2535 2046 1249 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1981 4339 4649 4795 5941 127
Size: 1854 2358 310 146 1146 317
Fragments arranged by size:
2358 1854 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 811 3143 811
Size: 2332 3799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1375 1618 1772 2051 2548 2886 3956 4388
Size: 621 243 154 279 497 338 1070 432
Cuts at: 4388 5135 5403 754
Size: 747 268 1482
Fragments arranged by size:
1482 1070 747 621 497 432 338 279
268 243 154
EcoNI CCTnn'n_nnAGG
Cuts at: 2500 2500
Size: 6131
EcoO109I rG'GnC_Cy
Cuts at: 2430 2570 3270 2430
Size: 140 700 5291
Fragments arranged by size:
5291 700 140
EcoRI G'AATT_C
Cuts at: 130 844 1619 1815 2038 3135 130
Size: 714 775 196 223 1097 3126
Fragments arranged by size:
3126 1097 775 714 223 196
EcoRV GAT'ATC
Cuts at: 407 609 1257 407
Size: 202 648 5281
Fragments arranged by size:
5281 648 202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 162 1069 4150 162
Size: 907 3081 2143
Fragments arranged by size:
3081 2143 907
FspI TGC'GCA
Cuts at: 3912 3912
Size: 6131
HaeII r_GCGC'y
Cuts at: 1158 1707 2307 2982 3565 3813 4835 5814 1158
Size: 549 600 675 583 248 1022 979 1475
Fragments arranged by size:
1475 1022 979 675 600 583 549 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 227 259 1161 1193 1879 1911 1956
Size: 32 44 32 902 32 686 32 45
Cuts at: 1956 1988 2790 2822 3973 4003 4005 4035 151
Size: 32 802 32 1151 30 2 30 2247
Fragments arranged by size:
2247 1151 902 802 686 45 44 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1434 3170 1434
Size: 1736 4395
HindIII A'AGCT_T
Cuts at: 545 705 545
Size: 160 5971
KasI G'GCGC_C
Cuts at: 3561 3809 3561
Size: 248 5883
KpnI G_GTAC'C
Cuts at: 3151 3151
Size: 6131
MfeI C'AATT_G
Cuts at: 293 293
Size: 6131
MluI A'CGCG_T
Cuts at: 5564 5564
Size: 6131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 1147 2925 4802 4986 554
Size: 593 1778 1877 184 1699
Fragments arranged by size:
1877 1778 1699 593 184
MscI TGG'CCA
Cuts at: 187 1069 2338 2394 3892 187
Size: 882 1269 56 1498 2426
Fragments arranged by size:
2426 1498 1269 882 56
MslI CAynn'nnrTG
Cuts at: 4120 4247 4120
Size: 127 6004
MspA1I CmG'CkG
Cuts at: 1347 1380 3242 3556 3916 4929 5174 1347
Size: 33 1862 314 360 1013 245 2304
Fragments arranged by size:
2304 1862 1013 360 314 245 33
NaeI GCC'GGC
Cuts at: 1927 4312 1927
Size: 2385 3746
NarI GG'CG_CC
Cuts at: 3562 3810 3562
Size: 248 5883
NcoI C'CATG_G
Cuts at: 107 1224 2349 107
Size: 1117 1125 3889
Fragments arranged by size:
3889 1125 1117
NgoMIV G'CCGG_C
Cuts at: 1925 4310 1925
Size: 2385 3746
NspI r_CATG'y
Cuts at: 2256 3184 3684 4591 2256
Size: 928 500 907 3796
Fragments arranged by size:
3796 928 907 500
PciI A'CATG_T
Cuts at: 4587 4587
Size: 6131
PflMI CCAn_nnn'nTGG
Cuts at: 2334 2334
Size: 6131
PmeI GTTT'AAAC
Cuts at: 3130 3130
Size: 6131
PmlI CAC'GTG
Cuts at: 2385 2385
Size: 6131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5270 5302 5270
Size: 32 6099
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 737 769 2154 2186 737
Size: 32 1385 32 4682
Fragments arranged by size:
4682 1385 32 32
PstI C_TGCA'G
Cuts at: 1331 2438 3178 3863 1331
Size: 1107 740 685 3599
Fragments arranged by size:
3599 1107 740 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6131
PvuII CAG'CTG
Cuts at: 1347 3556 3916 1347
Size: 2209 360 3562
Fragments arranged by size:
3562 2209 360
RsrII CG'GwC_CG
Cuts at: 4326 4326
Size: 6131
SacI G_AGCT'C
Cuts at: 813 3145 813
Size: 2332 3799
SalI G'TCGA_C
Cuts at: 3168 3168
Size: 6131
SapI GCTCTTCn'nnn_
Cuts at: 4154 4364 4154
Size: 210 5921
SbfI CC_TGCA'GG
Cuts at: 3178 3178
Size: 6131
ScaI AGT'ACT
Cuts at: 5648 5648
Size: 6131
SfcI C'TryA_G
Cuts at: 33 1327 2434 3174 3859 4852 5043 33
Size: 1294 1107 740 685 993 191 1121
Fragments arranged by size:
1294 1121 1107 993 740 685 191
SfoI GGC'GCC
Cuts at: 3563 3811 3563
Size: 248 5883
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6131
SmaI CCC'GGG
Cuts at: 3120 3153 3120
Size: 33 6098
SmlI C'TyrA_G
Cuts at: 523 582 674 1735 3286 4201 4693 4955 523
Size: 59 92 1061 1551 915 492 262 1699
Fragments arranged by size:
1699 1551 1061 915 492 262 92 59
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6131
SphI G_CATG'C
Cuts at: 2256 3184 3684 2256
Size: 928 500 4703
Fragments arranged by size:
4703 928 500
StyI C'CwwG_G
Cuts at: 107 732 1139 1224 2174 2270 2349 3265 3619
Size: 625 407 85 950 96 79 916 354
Cuts at: 3619 107
Size: 2619
Fragments arranged by size:
2619 950 916 625 407 354 96 85
79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3714 3847 4306 4515 3714
Size: 133 459 209 5330
Fragments arranged by size:
5330 459 209 133
TatI w'GTAC_w
Cuts at: 446 2783 3009 5646 446
Size: 2337 226 2637 931
Fragments arranged by size:
2637 2337 931 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2939 2971 5648 5680 2939
Size: 32 2677 32 3390
Fragments arranged by size:
3390 2677 32 32
Tth111I GACn'n_nGTC
Cuts at: 163 3928 163
Size: 3765 2366
XbaI T'CTAG_A
Cuts at: 3162 3162
Size: 6131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 537 3626 537
Size: 3089 3042
XhoI C'TCGA_G
Cuts at: 674 674
Size: 6131
XmaI C'CCGG_G
Cuts at: 3118 3151 3118
Size: 33 6098
XmnI GAAnn'nnTTC
Cuts at: 1517 2962 1517
Size: 1445 4686
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmtI BsiWI BsrGI BssHII
BstXI BstZ17I FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI