Restriction map for
pF1KA0713
(Circular) (Six-base) MAPSORT of: pF1KA0713.seq Check: 9732 from: 1 to: 5984
pF1KA0713 5984 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1244 3022 1244
Size: 1778 4206
Acc65I G'GTAC_C
Cuts at: 3000 3000
Size: 5984
AclI AA'CG_TT
Cuts at: 2613 5824 2613
Size: 3211 2773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1599 2761 3809 4241 4594 5256 1599
Size: 1162 1048 432 353 662 2327
Fragments arranged by size:
2327 1162 1048 662 432 353
AflIII A'CryG_T
Cuts at: 1658 3336 5136 5417 1658
Size: 1678 1800 281 2225
Fragments arranged by size:
2225 1800 1678 281
AlwNI CAG_nnn'CTG
Cuts at: 1432 2064 2368 2656 3289 4727 1432
Size: 632 304 288 633 1438 2689
Fragments arranged by size:
2689 1438 633 632 304 288
ApaLI G'TGCA_C
Cuts at: 747 4822 747
Size: 4075 1909
ApoI r'AATT_y
Cuts at: 878 1439 1737 2878 2988 5337 5593 878
Size: 561 298 1141 110 2349 256 1269
Fragments arranged by size:
2349 1269 1141 561 298 256 110
AseI AT'TA_AT
Cuts at: 20 892 923 2546 20
Size: 872 31 1623 3458
Fragments arranged by size:
3458 1623 872 31
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
AvaI C'yCGr_G
Cuts at: 3004 3004
Size: 5984
BamHI G'GATC_C
Cuts at: 1684 3009 1684
Size: 1325 4659
BanI G'GyrC_C
Cuts at: 394 2334 3000 3414 3662 3697 394
Size: 1940 666 414 248 35 2681
Fragments arranged by size:
2681 1940 666 414 248 35
BanII G_rGCy'C
Cuts at: 2998 4028 2998
Size: 1030 4954
BbeI G_GCGC'C
Cuts at: 3418 3666 3418
Size: 248 5736
BbsI GAAGACnn'nnnn_
Cuts at: 2829 2829
Size: 5984
BciVI GTATCCnnnnn_n'
Cuts at: 1324 3882 4938 5984 1324
Size: 2558 1056 1046 1324
Fragments arranged by size:
2558 1324 1056 1046
BclI T'GATC_A
Cuts at: 657 1824 3504 657
Size: 1167 1680 3137
Fragments arranged by size:
3137 1680 1167
BfrBI ATG'CAT
Cuts at: 1843 2050 2626 1843
Size: 207 576 5201
Fragments arranged by size:
5201 576 207
BglII A'GATC_T
Cuts at: 681 1060 3499 5536 681
Size: 379 2439 2037 1129
Fragments arranged by size:
2439 2037 1129 379
BlpI GC'TnA_GC
Cuts at: 1649 3096 1649
Size: 1447 4537
Bme1580I G_kGCm'C
Cuts at: 751 3609 3702 4826 751
Size: 2858 93 1124 1909
Fragments arranged by size:
2858 1909 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3196 3277 3612 5756 3196
Size: 81 335 2144 3424
Fragments arranged by size:
3424 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4351 4351
Size: 5984
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 842 1310 403
Size: 439 468 5077
Fragments arranged by size:
5077 468 439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 1128 1950 2146 2939 3160 4039 4753 5051
Size: 666 822 196 793 221 879 714 298
Cuts at: 5051 462
Size: 1395
Fragments arranged by size:
1395 879 822 793 714 666 298 221
196
BsaI GGTCTCn'nnnn_
Cuts at: 1724 5688 1724
Size: 3964 2020
BsaAI yAC'GTr
Cuts at: 3337 3337
Size: 5984
BsaBI GATnn'nnATC
Cuts at: 6 3498 5535 6
Size: 3492 2037 455
Fragments arranged by size:
3492 2037 455
BsaHI Gr'CG_yC
Cuts at: 3415 3663 4365 5861 3415
Size: 248 702 1496 3538
Fragments arranged by size:
3538 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3173 3694 4372 4783 4930 5204 3173
Size: 521 678 411 147 274 3953
Fragments arranged by size:
3953 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1900 1900
Size: 5984
BseYI C'CCAG_C
Cuts at: 2094 3409 4167 4832 5240 2094
Size: 1315 758 665 408 2838
Fragments arranged by size:
2838 1315 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3526 3572 4802 104
Size: 3422 46 1230 1286
Fragments arranged by size:
3422 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 751 2998 3776 3966 4826 751
Size: 2247 778 190 860 1909
Fragments arranged by size:
2247 1909 860 778 190
BsmI GAATG_Cn'
Cuts at: 1235 1629 2904 1235
Size: 394 1275 4315
Fragments arranged by size:
4315 1275 394
Bsp1286I G_dGCh'C
Cuts at: 751 2998 3609 3702 3776 3966 4028 4826 751
Size: 2247 611 93 74 190 62 798 1909
Fragments arranged by size:
2247 1909 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1527 4416 1527
Size: 2889 3095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3034 3248 3550 3931 3034
Size: 214 302 381 5087
Fragments arranged by size:
5087 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4276 4330 5814 5979 50
Size: 4226 54 1484 165 55
Fragments arranged by size:
4226 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3896 3896
Size: 5984
BsrFI r'CCGG_y
Cuts at: 1865 3982 4163 1865
Size: 2117 181 3686
Fragments arranged by size:
3686 2117 181
BsrGI T'GTAC_A
Cuts at: 1860 1860
Size: 5984
BssSI C'ACGA_G
Cuts at: 872 4255 4963 872
Size: 3383 708 1893
Fragments arranged by size:
3383 1893 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 3361 1849
Size: 1512 4472
BstBI TT'CG_AA
Cuts at: 2682 4345 2682
Size: 1663 4321
BstEII G'GTnAC_C
Cuts at: 1338 1338
Size: 5984
BstYI r'GATC_y
Cuts at: 1 681 1060 1533 1668 1684 2040 3009 3476
Size: 680 379 473 135 16 356 969 467
Cuts at: 3476 3499 3834 4080 4386 4398 4484 5536 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 969 680 473 467 449 379 356
335 306 246 135 86 23 16 12
Bsu36I CC'TnA_GG
Cuts at: 778 778
Size: 5984
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 494 2359 3934 4115 4278 494
Size: 1865 1575 181 163 2200
Fragments arranged by size:
2200 1865 1575 181 163
BtsI GCAGTG_nn'
Cuts at: 3227 3227
Size: 5984
DraI TTT'AAA
Cuts at: 2983 2983
Size: 5984
DraIII CAC_nnn'GTG
Cuts at: 2112 2112
Size: 5984
DrdI GACnn_nn'nnGTC
Cuts at: 2268 3690 5034 2268
Size: 1422 1344 3218
Fragments arranged by size:
3218 1422 1344
EaeI y'GGCC_r
Cuts at: 2632 3569 3743 4134 4161 5506 2632
Size: 937 174 391 27 1345 3110
Fragments arranged by size:
3110 1345 937 391 174 27
EagI C'GGCC_G
Cuts at: 3569 3569
Size: 5984
EarI CTCTTCn'nnn_
Cuts at: 521 4007 4217 521
Size: 3486 210 2288
Fragments arranged by size:
3486 2288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4192 4934 5080 5794 4192
Size: 742 146 714 4382
Fragments arranged by size:
4382 742 714 146
EcoICRI GAG'CTC
Cuts at: 2996 2996
Size: 5984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 842 1310 1599 2761 3809 4241 4594 5256
Size: 439 468 289 1162 1048 432 353 662
Cuts at: 5256 403
Size: 1131
Fragments arranged by size:
1162 1131 1048 662 468 439 432 353
289
EcoO109I rG'GnC_Cy
Cuts at: 3123 3123
Size: 5984
EcoRI G'AATT_C
Cuts at: 878 2988 878
Size: 2110 3874
EcoRV GAT'ATC
Cuts at: 1086 1086
Size: 5984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2797 4003 2797
Size: 1206 4778
FspI TGC'GCA
Cuts at: 1639 3765 1639
Size: 2126 3858
FspAI rTGC'GCAy
Cuts at: 1639 1639
Size: 5984
HaeII r_GCGC'y
Cuts at: 2962 3418 3666 4896 5667 2962
Size: 456 248 1230 771 3279
Fragments arranged by size:
3279 1230 771 456 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 839 871 1690 1722 3826 3856 3858
Size: 32 335 32 819 32 2104 30 2
Cuts at: 3858 3888 472
Size: 30 2568
Fragments arranged by size:
2568 2104 819 335 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 113 2562 2571 3023 113
Size: 2449 9 452 3074
Fragments arranged by size:
3074 2449 452 9
HpaI GTT'AAC
Cuts at: 113 113
Size: 5984
KasI G'GCGC_C
Cuts at: 3414 3662 3414
Size: 248 5736
KpnI G_GTAC'C
Cuts at: 3004 3004
Size: 5984
MluI A'CGCG_T
Cuts at: 5417 5417
Size: 5984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 337 1416 1561 4743 4927 337
Size: 1079 145 3182 184 1394
Fragments arranged by size:
3182 1394 1079 184 145
MscI TGG'CCA
Cuts at: 2634 3745 2634
Size: 1111 4873
MslI CAynn'nnrTG
Cuts at: 940 3973 4100 940
Size: 3033 127 2824
Fragments arranged by size:
3033 2824 127
MspA1I CmG'CkG
Cuts at: 1075 1429 3095 3409 3769 4553 4798 1075
Size: 354 1666 314 360 784 245 2261
Fragments arranged by size:
2261 1666 784 360 354 314 245
NaeI GCC'GGC
Cuts at: 4165 4165
Size: 5984
NarI GG'CG_CC
Cuts at: 3415 3663 3415
Size: 248 5736
NdeI CA'TA_TG
Cuts at: 133 937 1839 2532 133
Size: 804 902 693 3585
Fragments arranged by size:
3585 902 804 693
NgoMIV G'CCGG_C
Cuts at: 4163 4163
Size: 5984
NsiI A_TGCA'T
Cuts at: 1845 2052 2628 1845
Size: 207 576 5201
Fragments arranged by size:
5201 576 207
NspI r_CATG'y
Cuts at: 1281 1644 1662 3037 3537 5140 1281
Size: 363 18 1375 500 1603 2125
Fragments arranged by size:
2125 1603 1375 500 363 18
PciI A'CATG_T
Cuts at: 1658 5136 1658
Size: 3478 2506
PfoI T'CCnGG_A
Cuts at: 819 1983 819
Size: 1164 4820
PmeI GTTT'AAAC
Cuts at: 2983 2983
Size: 5984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 4430
Size: 32 5952
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2122 2154 2122
Size: 32 5952
PstI C_TGCA'G
Cuts at: 3031 3716 3031
Size: 685 5299
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5984
PvuII CAG'CTG
Cuts at: 1075 1429 3409 3769 1075
Size: 354 1980 360 3290
Fragments arranged by size:
3290 1980 360 354
RsrII CG'GwC_CG
Cuts at: 4179 4179
Size: 5984
SacI G_AGCT'C
Cuts at: 2998 2998
Size: 5984
SalI G'TCGA_C
Cuts at: 3021 3021
Size: 5984
SapI GCTCTTCn'nnn_
Cuts at: 4007 4217 4007
Size: 210 5774
SbfI CC_TGCA'GG
Cuts at: 3031 3031
Size: 5984
ScaI AGT'ACT
Cuts at: 5501 5501
Size: 5984
SfcI C'TryA_G
Cuts at: 33 1724 2139 3027 3712 4680 4871 33
Size: 1691 415 888 685 968 191 1146
Fragments arranged by size:
1691 1146 968 888 685 415 191
SfoI GGC'GCC
Cuts at: 3416 3664 3416
Size: 248 5736
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
SgrAI Cr'CCGG_yG
Cuts at: 1865 1865
Size: 5984
SmaI CCC'GGG
Cuts at: 3006 3006
Size: 5984
SmlI C'TyrA_G
Cuts at: 441 1143 1965 2125 2954 3139 4054 4768 5030
Size: 702 822 160 829 185 915 714 262
Cuts at: 5030 441
Size: 1395
Fragments arranged by size:
1395 915 829 822 714 702 262 185
160
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5984
SphI G_CATG'C
Cuts at: 3037 3537 3037
Size: 500 5484
SspI AAT'ATT
Cuts at: 194 1005 2418 194
Size: 811 1413 3760
Fragments arranged by size:
3760 1413 811
StuI AGG'CCT
Cuts at: 973 973
Size: 5984
StyI C'CwwG_G
Cuts at: 150 3118 3472 150
Size: 2968 354 2662
Fragments arranged by size:
2968 2662 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 3567 3700 4159 4368 217
Size: 3350 133 459 209 1833
Fragments arranged by size:
3350 1833 459 209 133
TatI w'GTAC_w
Cuts at: 1782 1860 5499 1782
Size: 78 3639 2267
Fragments arranged by size:
3639 2267 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 933 965 5501 5533 933
Size: 32 4536 32 1384
Fragments arranged by size:
4536 1384 32 32
Tth111I GACn'n_nGTC
Cuts at: 3781 3781
Size: 5984
XbaI T'CTAG_A
Cuts at: 1065 3015 1065
Size: 1950 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3479 3479
Size: 5984
XmaI C'CCGG_G
Cuts at: 3004 3004
Size: 5984
XmnI GAAnn'nnTTC
Cuts at: 491 2614 2798 491
Size: 2123 184 3677
Fragments arranged by size:
3677 2123 184
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI
BssHII BstXI BstZ17I BtgI ClaI CspCI EcoNI FseI
HindIII MfeI NcoI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SnaBI SrfI SwaI XhoI ZraI