Restriction map for
pF1KA0698
(Circular) (Six-base) MAPSORT of: pF1KA0698.seq Check: 7706 from: 1 to: 5786
pF1KA0698 5786 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1250 1250
Size: 5786
AccI GT'mk_AC
Cuts at: 2824 2824
Size: 5786
Acc65I G'GTAC_C
Cuts at: 328 2699 2802 328
Size: 2371 103 3312
Fragments arranged by size:
3312 2371 103
AclI AA'CG_TT
Cuts at: 5626 5626
Size: 5786
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 3611 4043 4396 5058 1291
Size: 2320 432 353 662 2019
Fragments arranged by size:
2320 2019 662 432 353
AflII C'TTAA_G
Cuts at: 2511 2511
Size: 5786
AflIII A'CryG_T
Cuts at: 155 171 1211 1979 3138 4938 5219 155
Size: 16 1040 768 1159 1800 281 722
Fragments arranged by size:
1800 1159 1040 768 722 281 16
AleI CACnn'nnGTG
Cuts at: 268 295 268
Size: 27 5759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1315 2212 1315
Size: 897 4889
AlwNI CAG_nnn'CTG
Cuts at: 243 1264 3091 4529 243
Size: 1021 1827 1438 1500
Fragments arranged by size:
1827 1500 1438 1021
ApaI G_GGCC'C
Cuts at: 973 1922 2003 973
Size: 949 81 4756
Fragments arranged by size:
4756 949 81
ApaLI G'TGCA_C
Cuts at: 161 4624 161
Size: 4463 1323
ApoI r'AATT_y
Cuts at: 138 519 954 1036 2065 2790 5139 5395 138
Size: 381 435 82 1029 725 2349 256 529
Fragments arranged by size:
2349 1029 725 529 435 381 256 82
AseI AT'TA_AT
Cuts at: 20 1175 20
Size: 1155 4631
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5786
AvaI C'yCGr_G
Cuts at: 2806 2806
Size: 5786
BamHI G'GATC_C
Cuts at: 897 1691 1965 2145 2811 897
Size: 794 274 180 666 3872
Fragments arranged by size:
3872 794 666 274 180
BanI G'GyrC_C
Cuts at: 312 328 994 1238 2699 2802 3216 3464 3499
Size: 16 666 244 1461 103 414 248 35
Cuts at: 3499 312
Size: 2599
Fragments arranged by size:
2599 1461 666 414 248 244 103 35
16
BanII G_rGCy'C
Cuts at: 568 973 1634 1922 2003 2036 2800 3830 568
Size: 405 661 288 81 33 764 1030 2524
Fragments arranged by size:
2524 1030 764 661 405 288 81 33
BbeI G_GCGC'C
Cuts at: 3220 3468 3220
Size: 248 5538
BbsI GAAGACnn'nnnn_
Cuts at: 711 711
Size: 5786
BbvCI CC'TCA_GC
Cuts at: 692 692
Size: 5786
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5752
BciVI GTATCCnnnnn_n'
Cuts at: 3684 4740 5786 3684
Size: 1056 1046 3684
Fragments arranged by size:
3684 1056 1046
BclI T'GATC_A
Cuts at: 3306 3306
Size: 5786
BfrBI ATG'CAT
Cuts at: 111 642 1167 2199 111
Size: 531 525 1032 3698
Fragments arranged by size:
3698 1032 531 525
BglI GCCn_nnn'nGGC
Cuts at: 968 1409 2021 968
Size: 441 612 4733
Fragments arranged by size:
4733 612 441
BglII A'GATC_T
Cuts at: 3301 5338 3301
Size: 2037 3749
BlpI GC'TnA_GC
Cuts at: 1862 2898 1862
Size: 1036 4750
Bme1580I G_kGCm'C
Cuts at: 165 317 586 973 1258 1922 2003 2558 3411
Size: 152 269 387 285 664 81 555 853
Cuts at: 3411 3504 4628 165
Size: 93 1124 1323
Fragments arranged by size:
1323 1124 853 664 555 387 285 269
152 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 521 1449 2998 3079 3414 5558 521
Size: 928 1549 81 335 2144 749
Fragments arranged by size:
2144 1549 928 749 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4153 4153
Size: 5786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 2050 552
Size: 1498 4288
Bpu10I CC'TnA_GC
Cuts at: 146 692 893 1615 146
Size: 546 201 722 4317
Fragments arranged by size:
4317 722 546 201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2962 3841 4555 4853 2962
Size: 879 714 298 3895
Fragments arranged by size:
3895 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2470 5490 2470
Size: 3020 2766
BsaAI yAC'GTr
Cuts at: 3139 3139
Size: 5786
BsaBI GATnn'nnATC
Cuts at: 6 659 2297 3300 5337 6
Size: 653 1638 1003 2037 455
Fragments arranged by size:
2037 1638 1003 653 455
BsaHI Gr'CG_yC
Cuts at: 3217 3465 4167 5663 3217
Size: 248 702 1496 3340
Fragments arranged by size:
3340 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1553 2975 3496 4174 4585 4732 5006 1553
Size: 1422 521 678 411 147 274 2333
Fragments arranged by size:
2333 1422 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 718 748 2246 2276 718
Size: 30 1498 30 4228
Fragments arranged by size:
4228 1498 30 30
BseYI C'CCAG_C
Cuts at: 737 1559 2592 3211 3969 4634 5042 737
Size: 822 1033 619 758 665 408 1481
Fragments arranged by size:
1481 1033 822 758 665 619 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 1025 1321 1346 1005
Size: 20 296 25 5445
Fragments arranged by size:
5445 296 25 20
BsiEI CG_ry'CG
Cuts at: 104 3328 3374 4604 104
Size: 3224 46 1230 1286
Fragments arranged by size:
3224 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 165 568 2800 3578 3768 4628 165
Size: 403 2232 778 190 860 1323
Fragments arranged by size:
2232 1323 860 778 403 190
Bsp1286I G_dGCh'C
Cuts at: 165 317 568 586 973 1258 1634 1922 2003
Size: 152 251 18 387 285 376 288 81
Cuts at: 2003 2036 2558 2800 3411 3504 3578 3768 3830
Size: 33 522 242 611 93 74 190 62
Cuts at: 3830 4628 165
Size: 798 1323
Fragments arranged by size:
1323 798 611 522 387 376 288 285
251 242 190 152 93 81 74 62
33 18
BspHI T'CATG_A
Cuts at: 4218 4218
Size: 5786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 434 1205 1250 2836 3050 3352 3733 434
Size: 771 45 1586 214 302 381 2487
Fragments arranged by size:
2487 1586 771 381 302 214 45
BsrBI CCG'CTC
Cuts at: 50 4078 4132 5616 5781 50
Size: 4028 54 1484 165 55
Fragments arranged by size:
4028 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 207 1092 2586 3698 207
Size: 885 1494 1112 2295
Fragments arranged by size:
2295 1494 1112 885
BsrFI r'CCGG_y
Cuts at: 880 3784 3965 880
Size: 2904 181 2701
Fragments arranged by size:
2904 2701 181
BsrGI T'GTAC_A
Cuts at: 797 797
Size: 5786
BssSI C'ACGA_G
Cuts at: 4057 4765 4057
Size: 708 5078
BstAPI GCAn_nnn'nTGC
Cuts at: 3163 3163
Size: 5786
BstBI TT'CG_AA
Cuts at: 4147 4147
Size: 5786
BstXI CCAn_nnnn'nTGG
Cuts at: 714 1462 714
Size: 748 5038
BstYI r'GATC_y
Cuts at: 1 897 936 1691 1946 1965 2145 2298 2373
Size: 896 39 755 255 19 180 153 75
Cuts at: 2373 2603 2811 3278 3301 3636 3882 4188 4200
Size: 230 208 467 23 335 246 306 12
Cuts at: 4200 4286 5338 1
Size: 86 1052 449
Fragments arranged by size:
1052 896 755 467 449 335 306 255
246 230 208 180 153 86 75 39
23 19 12
Bsu36I CC'TnA_GG
Cuts at: 2230 2230
Size: 5786
BtgI C'CryG_G
Cuts at: 231 252 407 759 2037 2133 2282 231
Size: 21 155 352 1278 96 149 3735
Fragments arranged by size:
3735 1278 352 155 149 96 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3736 3917 4080 3736
Size: 181 163 5442
Fragments arranged by size:
5442 181 163
BtsI GCAGTG_nn'
Cuts at: 887 1967 2439 2558 3029 887
Size: 1080 472 119 471 3644
Fragments arranged by size:
3644 1080 472 471 119
DraI TTT'AAA
Cuts at: 2785 2785
Size: 5786
DrdI GACnn_nn'nnGTC
Cuts at: 3492 4836 3492
Size: 1344 4442
EaeI y'GGCC_r
Cuts at: 1358 1464 1699 1849 2279 2411 3371 3545 3936
Size: 106 235 150 430 132 960 174 391
Cuts at: 3936 3963 5308 1358
Size: 27 1345 1836
Fragments arranged by size:
1836 1345 960 430 391 235 174 150
132 106 27
EagI C'GGCC_G
Cuts at: 3371 3371
Size: 5786
EarI CTCTTCn'nnn_
Cuts at: 556 1054 2488 3809 4019 556
Size: 498 1434 1321 210 2323
Fragments arranged by size:
2323 1434 1321 498 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3994 4736 4882 5596 3994
Size: 742 146 714 4184
Fragments arranged by size:
4184 742 714 146
EcoICRI GAG'CTC
Cuts at: 566 2798 566
Size: 2232 3554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 1291 2050 3611 4043 4396 5058 552
Size: 739 759 1561 432 353 662 1280
Fragments arranged by size:
1561 1280 759 739 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1999 2000 2138 2925 1999
Size: 1 138 787 4860
Fragments arranged by size:
4860 787 138 1
EcoRI G'AATT_C
Cuts at: 1036 2790 1036
Size: 1754 4032
EcoRV GAT'ATC
Cuts at: 1767 1767
Size: 5786
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3805 3805
Size: 5786
FspI TGC'GCA
Cuts at: 3567 3567
Size: 5786
HaeII r_GCGC'y
Cuts at: 3220 3468 4698 5469 3220
Size: 248 1230 771 3537
Fragments arranged by size:
3537 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 796 828 3628 3658 3660 3690 796
Size: 32 2800 30 2 30 2892
Fragments arranged by size:
2892 2800 32 30 30 2
HincII GTy'rAC
Cuts at: 2825 2825
Size: 5786
HindIII A'AGCT_T
Cuts at: 2761 2761
Size: 5786
KasI G'GCGC_C
Cuts at: 3216 3464 3216
Size: 248 5538
KpnI G_GTAC'C
Cuts at: 332 2703 2806 332
Size: 2371 103 3312
Fragments arranged by size:
3312 2371 103
MluI A'CGCG_T
Cuts at: 5219 5219
Size: 5786
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1040 1091 1143 4545 4729 1040
Size: 51 52 3402 184 2097
Fragments arranged by size:
3402 2097 184 52 51
MscI TGG'CCA
Cuts at: 1360 1466 1851 2281 2413 3547 1360
Size: 106 385 430 132 1134 3599
Fragments arranged by size:
3599 1134 430 385 132 106
MslI CAynn'nnrTG
Cuts at: 266 268 295 712 916 1820 2318 2444 2462
Size: 2 27 417 204 904 498 126 18
Cuts at: 2462 3775 3902 266
Size: 1313 127 2150
Fragments arranged by size:
2150 1313 904 498 417 204 127 126
27 18 2
MspA1I CmG'CkG
Cuts at: 860 975 1102 2897 3211 3571 4355 4600 860
Size: 115 127 1795 314 360 784 245 2046
Fragments arranged by size:
2046 1795 784 360 314 245 127 115
NaeI GCC'GGC
Cuts at: 3967 3967
Size: 5786
NarI GG'CG_CC
Cuts at: 3217 3465 3217
Size: 248 5538
NcoI C'CATG_G
Cuts at: 231 407 759 2037 2133 2282 231
Size: 176 352 1278 96 149 3735
Fragments arranged by size:
3735 1278 352 176 149 96
NgoMIV G'CCGG_C
Cuts at: 3965 3965
Size: 5786
NsiI A_TGCA'T
Cuts at: 113 644 1169 2201 113
Size: 531 525 1032 3698
Fragments arranged by size:
3698 1032 531 525
NspI r_CATG'y
Cuts at: 115 159 378 753 1171 1215 1819 1983 2203
Size: 44 219 375 418 44 604 164 220
Cuts at: 2203 2277 2601 2839 3339 4942 115
Size: 74 324 238 500 1603 959
Fragments arranged by size:
1603 959 604 500 418 375 324 238
220 219 164 74 44 44
PasI CC'CwG_GG
Cuts at: 317 1915 2383 2422 317
Size: 1598 468 39 3681
Fragments arranged by size:
3681 1598 468 39
PciI A'CATG_T
Cuts at: 155 1211 1979 4938 155
Size: 1056 768 2959 1003
Fragments arranged by size:
2959 1056 1003 768
PflMI CCAn_nnn'nTGG
Cuts at: 1708 2295 1708
Size: 587 5199
PfoI T'CCnGG_A
Cuts at: 1942 2027 2744 1942
Size: 85 717 4984
Fragments arranged by size:
4984 717 85
PmeI GTTT'AAAC
Cuts at: 2785 2785
Size: 5786
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4232 4264 4232
Size: 32 5754
PpuMI rG'GwC_Cy
Cuts at: 2138 2138
Size: 5786
PspOMI G'GGCC_C
Cuts at: 969 1918 1999 969
Size: 949 81 4756
Fragments arranged by size:
4756 949 81
PstI C_TGCA'G
Cuts at: 936 1962 2833 3518 936
Size: 1026 871 685 3204
Fragments arranged by size:
3204 1026 871 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5786
PvuII CAG'CTG
Cuts at: 1102 3211 3571 1102
Size: 2109 360 3317
Fragments arranged by size:
3317 2109 360
RsrII CG'GwC_CG
Cuts at: 3981 3981
Size: 5786
SacI G_AGCT'C
Cuts at: 568 2800 568
Size: 2232 3554
SalI G'TCGA_C
Cuts at: 2823 2823
Size: 5786
SanDI GG'GwC_CC
Cuts at: 2138 2138
Size: 5786
SapI GCTCTTCn'nnn_
Cuts at: 556 3809 4019 556
Size: 3253 210 2323
Fragments arranged by size:
3253 2323 210
SbfI CC_TGCA'GG
Cuts at: 2833 2833
Size: 5786
ScaI AGT'ACT
Cuts at: 451 510 5303 451
Size: 59 4793 934
Fragments arranged by size:
4793 934 59
SexAI A'CCwGG_T
Cuts at: 324 331 324
Size: 7 5779
SfcI C'TryA_G
Cuts at: 33 932 1958 2164 2829 3514 4482 4673 33
Size: 899 1026 206 665 685 968 191 1146
Fragments arranged by size:
1146 1026 968 899 685 665 206 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 968 968
Size: 5786
SfoI GGC'GCC
Cuts at: 3218 3466 3218
Size: 248 5538
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5786
SmaI CCC'GGG
Cuts at: 2808 2808
Size: 5786
SmlI C'TyrA_G
Cuts at: 2511 2941 3856 4570 4832 2511
Size: 430 915 714 262 3465
Fragments arranged by size:
3465 915 714 430 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5786
SphI G_CATG'C
Cuts at: 115 378 753 1171 1819 2203 2601 2839 3339
Size: 263 375 418 648 384 398 238 500
Cuts at: 3339 115
Size: 2562
Fragments arranged by size:
2562 648 500 418 398 384 375 263
238
StyI C'CwwG_G
Cuts at: 231 407 759 998 1380 1702 2003 2037 2133
Size: 176 352 239 382 322 301 34 96
Cuts at: 2133 2250 2282 2920 3274 231
Size: 117 32 638 354 2743
Fragments arranged by size:
2743 638 382 354 352 322 301 239
176 117 96 34 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 688 991 3369 3502 3961 4170 688
Size: 303 2378 133 459 209 2304
Fragments arranged by size:
2378 2304 459 303 209 133
TatI w'GTAC_w
Cuts at: 449 508 797 920 5301 449
Size: 59 289 123 4381 934
Fragments arranged by size:
4381 934 289 123 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2246 2278 5303 5335 2246
Size: 32 3025 32 2697
Fragments arranged by size:
3025 2697 32 32
Tth111I GACn'n_nGTC
Cuts at: 2458 3583 2458
Size: 1125 4661
XbaI T'CTAG_A
Cuts at: 2817 2817
Size: 5786
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2464 3281 2464
Size: 817 4969
XmaI C'CCGG_G
Cuts at: 2806 2806
Size: 5786
XmnI GAAnn'nnTTC
Cuts at: 611 1040 611
Size: 429 5357
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AloI AscI AvrII BaeI
BmgBI BmtI BseRI BsiWI BsmI BsmBI BspEI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PshAI PsiI PspXI PsrI SacII SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI