Restriction map for
pF1KA0683
(Circular) (Six-base) MAPSORT of: pF1KA0683.seq Check: 2517 from: 1 to: 5624
pF1KA0683 5624 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1098 1098
Size: 5624
AccI GT'mk_AC
Cuts at: 1733 2662 1733
Size: 929 4695
Acc65I G'GTAC_C
Cuts at: 810 2640 810
Size: 1830 3794
AclI AA'CG_TT
Cuts at: 5464 5464
Size: 5624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1135 1645 2203 3449 3881 4234 4896 232
Size: 903 510 558 1246 432 353 662 960
Fragments arranged by size:
1246 960 903 662 558 510 432 353
AfeI AGC'GCT
Cuts at: 245 827 1696 2224 245
Size: 582 869 528 3645
Fragments arranged by size:
3645 869 582 528
AflIII A'CryG_T
Cuts at: 1604 2976 4776 5057 1604
Size: 1372 1800 281 2171
Fragments arranged by size:
2171 1800 1372 281
AhdI GACnn_n'nnGTC
Cuts at: 139 139
Size: 5624
AleI CACnn'nnGTG
Cuts at: 2332 2332
Size: 5624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 634 862 2374 634
Size: 228 1512 3884
Fragments arranged by size:
3884 1512 228
AlwNI CAG_nnn'CTG
Cuts at: 1059 1113 1974 2415 2929 4367 1059
Size: 54 861 441 514 1438 2316
Fragments arranged by size:
2316 1438 861 514 441 54
ApaI G_GGCC'C
Cuts at: 391 445 1719 2161 2307 2379 2568 391
Size: 54 1274 442 146 72 189 3447
Fragments arranged by size:
3447 1274 442 189 146 72 54
ApaLI G'TGCA_C
Cuts at: 4462 4462
Size: 5624
ApoI r'AATT_y
Cuts at: 1381 2365 2628 4977 5233 1381
Size: 984 263 2349 256 1772
Fragments arranged by size:
2349 1772 984 263 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5624
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
AvaI C'yCGr_G
Cuts at: 249 1219 1363 1411 1754 1993 2148 2644 249
Size: 970 144 48 343 239 155 496 3229
Fragments arranged by size:
3229 970 496 343 239 155 144 48
BamHI G'GATC_C
Cuts at: 1355 2649 1355
Size: 1294 4330
BanI G'GyrC_C
Cuts at: 810 866 1091 1453 1988 2640 3054 3302 3337
Size: 56 225 362 535 652 414 248 35
Cuts at: 3337 810
Size: 3097
Fragments arranged by size:
3097 652 535 414 362 248 225 56
35
BanII G_rGCy'C
Cuts at: 322 391 445 1499 1561 1719 1744 1845 2161
Size: 69 54 1054 62 158 25 101 316
Cuts at: 2161 2307 2379 2568 2638 3668 322
Size: 146 72 189 70 1030 2278
Fragments arranged by size:
2278 1054 1030 316 189 158 146 101
72 70 69 62 54 25
BbeI G_GCGC'C
Cuts at: 870 1095 1457 3058 3306 870
Size: 225 362 1601 248 3188
Fragments arranged by size:
3188 1601 362 248 225
BbsI GAAGACnn'nnnn_
Cuts at: 161 1810 2270 161
Size: 1649 460 3515
Fragments arranged by size:
3515 1649 460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 418 452 1029 1063 1458 1492 418
Size: 34 577 34 395 34 4550
Fragments arranged by size:
4550 577 395 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3522 4578 5624 3522
Size: 1056 1046 3522
Fragments arranged by size:
3522 1056 1046
BclI T'GATC_A
Cuts at: 3144 3144
Size: 5624
BglI GCCn_nnn'nGGC
Cuts at: 671 901 1064 1462 1714 1849 1879 2165 671
Size: 230 163 398 252 135 30 286 4130
Fragments arranged by size:
4130 398 286 252 230 163 135 30
BglII A'GATC_T
Cuts at: 3139 5176 3139
Size: 2037 3587
BlpI GC'TnA_GC
Cuts at: 316 2443 2736 316
Size: 2127 293 3204
Fragments arranged by size:
3204 2127 293
Bme1580I G_kGCm'C
Cuts at: 391 445 1353 1471 1719 2161 2307 2379 2568
Size: 54 908 118 248 442 146 72 189
Cuts at: 2568 3249 3342 4466 391
Size: 681 93 1124 1549
Fragments arranged by size:
1549 1124 908 681 442 248 189 146
118 93 72 54
BmgBI CAC'GTC
Cuts at: 1133 1185 1472 1679 2517 1133
Size: 52 287 207 838 4240
Fragments arranged by size:
4240 838 287 207 52
BmrI ACTGGGnnnn_n'
Cuts at: 943 987 2836 2917 3252 5396 943
Size: 44 1849 81 335 2144 1171
Fragments arranged by size:
2144 1849 1171 335 81 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 288 3991 288
Size: 3703 1921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 224 352 376 403 1816 2488 223
Size: 1 128 24 27 1413 672 3359
Fragments arranged by size:
3359 1413 672 128 27 24 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1741 2572 2800 3679 4393 4691 1741
Size: 831 228 879 714 298 2674
Fragments arranged by size:
2674 879 831 714 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 415 1501 5328 415
Size: 1086 3827 711
Fragments arranged by size:
3827 1086 711
BsaAI yAC'GTr
Cuts at: 2403 2977 2403
Size: 574 5050
BsaBI GATnn'nnATC
Cuts at: 6 3138 5175 6
Size: 3132 2037 455
Fragments arranged by size:
3132 2037 455
BsaHI Gr'CG_yC
Cuts at: 867 1042 1092 1317 1454 1535 2080 3055 3303
Size: 175 50 225 137 81 545 975 248
Cuts at: 3303 4005 5501 867
Size: 702 1496 990
Fragments arranged by size:
1496 990 975 702 545 248 225 175
137 81 50
BsaWI w'CCGG_w
Cuts at: 2813 3334 4012 4423 4570 4844 2813
Size: 521 678 411 147 274 3593
Fragments arranged by size:
3593 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 511 541 349
Size: 30 132 30 5432
Fragments arranged by size:
5432 132 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 373 691 1813 2119 2421 2590 277
Size: 96 318 1122 306 302 169 3311
Fragments arranged by size:
3311 1122 318 306 302 169 96
BseYI C'CCAG_C
Cuts at: 321 445 576 960 1062 1436 1697 2126 2173
Size: 124 131 384 102 374 261 429 47
Cuts at: 2173 3049 3807 4472 4880 321
Size: 876 758 665 408 1065
Fragments arranged by size:
1065 876 758 665 429 408 384 374
261 131 124 102 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 832 180
Size: 652 4972
BsiEI CG_ry'CG
Cuts at: 104 2426 3166 3212 4442 104
Size: 2322 740 46 1230 1286
Fragments arranged by size:
2322 1286 1230 740 46
BsiHKAI G_wGCw'C
Cuts at: 2638 3416 3606 4466 2638
Size: 778 190 860 3796
Fragments arranged by size:
3796 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 562 1123 2061 2433 2618 562
Size: 561 938 372 185 3568
Fragments arranged by size:
3568 938 561 372 185
Bsp1286I G_dGCh'C
Cuts at: 322 391 445 1353 1471 1499 1561 1719 1744
Size: 69 54 908 118 28 62 158 25
Cuts at: 1744 1845 2161 2307 2379 2568 2638 3249 3342
Size: 101 316 146 72 189 70 611 93
Cuts at: 3342 3416 3606 3668 4466 322
Size: 74 190 62 798 1480
Fragments arranged by size:
1480 908 798 611 316 190 189 158
146 118 101 93 74 72 70 69
62 62 54 28 25
BspHI T'CATG_A
Cuts at: 4056 4056
Size: 5624
BspMI ACCTGCnnnn'nnnn_
Cuts at: 854 1098 2674 2888 3190 3571 854
Size: 244 1576 214 302 381 2907
Fragments arranged by size:
2907 1576 381 302 244 214
BsrBI CCG'CTC
Cuts at: 50 1098 2109 3916 3970 5454 5619 50
Size: 1048 1011 1807 54 1484 165 55
Fragments arranged by size:
1807 1484 1048 1011 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3536 3536
Size: 5624
BsrFI r'CCGG_y
Cuts at: 500 2194 3622 3803 500
Size: 1694 1428 181 2321
Fragments arranged by size:
2321 1694 1428 181
BssHII G'CGCG_C
Cuts at: 2458 2458
Size: 5624
BssSI C'ACGA_G
Cuts at: 3895 4603 3895
Size: 708 4916
BstAPI GCAn_nnn'nTGC
Cuts at: 1059 3001 1059
Size: 1942 3682
BstBI TT'CG_AA
Cuts at: 3985 3985
Size: 5624
BstEII G'GTnAC_C
Cuts at: 2241 2241
Size: 5624
BstXI CCAn_nnnn'nTGG
Cuts at: 1269 1269
Size: 5624
BstYI r'GATC_y
Cuts at: 1 794 1355 2649 3116 3139 3474 3720 4026
Size: 793 561 1294 467 23 335 246 306
Cuts at: 4026 4038 4124 5176 1
Size: 12 86 1052 449
Fragments arranged by size:
1294 1052 793 561 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 941 2298 941
Size: 1357 4267
BtgI C'CryG_G
Cuts at: 107 345 777 896 1076 1487 2075 2189 2330
Size: 238 432 119 180 411 588 114 141
Cuts at: 2330 2345 107
Size: 15 3386
Fragments arranged by size:
3386 588 432 411 238 180 141 119
114 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1595 3574 3755 3918 1595
Size: 1979 181 163 3301
Fragments arranged by size:
3301 1979 181 163
BtsI GCAGTG_nn'
Cuts at: 515 532 1152 2076 2867 515
Size: 17 620 924 791 3272
Fragments arranged by size:
3272 924 791 620 17
ClaI AT'CG_AT
Cuts at: 2393 2393
Size: 5624
DraI TTT'AAA
Cuts at: 2623 2623
Size: 5624
DraIII CAC_nnn'GTG
Cuts at: 2036 2036
Size: 5624
DrdI GACnn_nn'nnGTC
Cuts at: 3330 4674 3330
Size: 1344 4280
EaeI y'GGCC_r
Cuts at: 183 348 479 635 1073 1259 1775 1850 1880
Size: 165 131 156 438 186 516 75 30
Cuts at: 1880 2192 2196 2342 2423 3209 3383 3774 3801
Size: 312 4 146 81 786 174 391 27
Cuts at: 3801 5146 183
Size: 1345 661
Fragments arranged by size:
1345 786 661 516 438 391 312 186
174 165 156 146 131 81 75 30
27 4
EagI C'GGCC_G
Cuts at: 2423 3209 2423
Size: 786 4838
EarI CTCTTCn'nnn_
Cuts at: 1388 1621 3647 3857 1388
Size: 233 2026 210 3155
Fragments arranged by size:
3155 2026 233 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 409 652 1144 2527 3832 4574 4720 5434 409
Size: 243 492 1383 1305 742 146 714 599
Fragments arranged by size:
1383 1305 742 714 599 492 243 146
EcoICRI GAG'CTC
Cuts at: 2636 2636
Size: 5624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 224 232 352 376 403 1135 1645 1816
Size: 1 8 120 24 27 732 510 171
Cuts at: 1816 2203 2488 3449 3881 4234 4896 223
Size: 387 285 961 432 353 662 951
Fragments arranged by size:
961 951 732 662 510 432 387 353
285 171 120 27 24 8 1
EcoNI CCTnn'n_nnAGG
Cuts at: 493 622 1105 2044 2467 493
Size: 129 483 939 423 3650
Fragments arranged by size:
3650 939 483 423 129
EcoO109I rG'GnC_Cy
Cuts at: 387 442 756 937 945 1369 1716 1725 2157
Size: 55 314 181 8 424 347 9 432
Cuts at: 2157 2303 2304 2355 2376 2472 2564 2565 2763
Size: 146 1 51 21 96 92 1 198
Cuts at: 2763 387
Size: 3248
Fragments arranged by size:
3248 432 424 347 314 198 181 146
96 92 55 51 21 9 8 1
1
EcoRI G'AATT_C
Cuts at: 2365 2628 2365
Size: 263 5361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 5624
FseI GG_CCGG'CC
Cuts at: 2198 2198
Size: 5624
FspI TGC'GCA
Cuts at: 3405 3405
Size: 5624
HaeII r_GCGC'y
Cuts at: 247 829 870 1095 1182 1457 1698 2226 3058
Size: 582 41 225 87 275 241 528 832
Cuts at: 3058 3306 4536 5307 247
Size: 248 1230 771 564
Fragments arranged by size:
1230 832 771 582 564 528 275 248
241 225 87 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 222 254 1441 1473 3466 3496 3498
Size: 32 65 32 1187 32 1993 30 2
Cuts at: 3498 3528 125
Size: 30 2221
Fragments arranged by size:
2221 1993 1187 65 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2325 2663 2325
Size: 338 5286
HindIII A'AGCT_T
Cuts at: 1012 1012
Size: 5624
HpaI GTT'AAC
Cuts at: 2325 2325
Size: 5624
KasI G'GCGC_C
Cuts at: 866 1091 1453 3054 3302 866
Size: 225 362 1601 248 3188
Fragments arranged by size:
3188 1601 362 248 225
KpnI G_GTAC'C
Cuts at: 814 2644 814
Size: 1830 3794
MluI A'CGCG_T
Cuts at: 5057 5057
Size: 5624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1106 4383 4567 1106
Size: 3277 184 2163
Fragments arranged by size:
3277 2163 184
MscI TGG'CCA
Cuts at: 481 1075 1261 1777 2344 3385 481
Size: 594 186 516 567 1041 2720
Fragments arranged by size:
2720 1041 594 567 516 186
MslI CAynn'nnrTG
Cuts at: 1330 2332 2393 3613 3740 1330
Size: 1002 61 1220 127 3214
Fragments arranged by size:
3214 1220 1002 127 61
MspA1I CmG'CkG
Cuts at: 1089 1551 2130 2271 2735 3049 3409 4193 4438
Size: 462 579 141 464 314 360 784 245
Cuts at: 4438 1089
Size: 2275
Fragments arranged by size:
2275 784 579 464 462 360 314 245
141
NaeI GCC'GGC
Cuts at: 502 2196 3805 502
Size: 1694 1609 2321
Fragments arranged by size:
2321 1694 1609
NarI GG'CG_CC
Cuts at: 867 1092 1454 3055 3303 867
Size: 225 362 1601 248 3188
Fragments arranged by size:
3188 1601 362 248 225
NcoI C'CATG_G
Cuts at: 107 345 896 1076 2345 107
Size: 238 551 180 1269 3386
Fragments arranged by size:
3386 1269 551 238 180
NgoMIV G'CCGG_C
Cuts at: 500 2194 3803 500
Size: 1694 1609 2321
Fragments arranged by size:
2321 1694 1609
NspI r_CATG'y
Cuts at: 1608 2677 3177 4780 1608
Size: 1069 500 1603 2452
Fragments arranged by size:
2452 1603 1069 500
PasI CC'CwG_GG
Cuts at: 834 834
Size: 5624
PciI A'CATG_T
Cuts at: 1604 4776 1604
Size: 3172 2452
PfoI T'CCnGG_A
Cuts at: 143 380 563 1652 1943 143
Size: 237 183 1089 291 3824
Fragments arranged by size:
3824 1089 291 237 183
PmeI GTTT'AAAC
Cuts at: 2623 2623
Size: 5624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1394 1426 4070 4102 1394
Size: 32 2644 32 2916
Fragments arranged by size:
2916 2644 32 32
PpuMI rG'GwC_Cy
Cuts at: 756 945 2472 756
Size: 189 1527 3908
Fragments arranged by size:
3908 1527 189
PspOMI G'GGCC_C
Cuts at: 387 441 1715 2157 2303 2375 2564 387
Size: 54 1274 442 146 72 189 3447
Fragments arranged by size:
3447 1274 442 189 146 72 54
PspXI vC'TCGA_Gb
Cuts at: 1411 1411
Size: 5624
PstI C_TGCA'G
Cuts at: 623 698 1055 1109 1506 2548 2582 2671 3356
Size: 75 357 54 397 1042 34 89 685
Cuts at: 3356 623
Size: 2891
Fragments arranged by size:
2891 1042 685 397 357 89 75 54
34
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5624
PvuII CAG'CTG
Cuts at: 1551 2271 3049 3409 1551
Size: 720 778 360 3766
Fragments arranged by size:
3766 778 720 360
RsrII CG'GwC_CG
Cuts at: 888 3819 888
Size: 2931 2693
SacI G_AGCT'C
Cuts at: 2638 2638
Size: 5624
SalI G'TCGA_C
Cuts at: 2661 2661
Size: 5624
SapI GCTCTTCn'nnn_
Cuts at: 1621 3647 3857 1621
Size: 2026 210 3388
Fragments arranged by size:
3388 2026 210
SbfI CC_TGCA'GG
Cuts at: 2671 2671
Size: 5624
ScaI AGT'ACT
Cuts at: 5141 5141
Size: 5624
SexAI A'CCwGG_T
Cuts at: 968 968
Size: 5624
SfcI C'TryA_G
Cuts at: 33 619 694 1051 1105 1502 1734 1917 2544
Size: 586 75 357 54 397 232 183 627
Cuts at: 2544 2578 2667 3352 4320 4511 33
Size: 34 89 685 968 191 1146
Fragments arranged by size:
1146 968 685 627 586 397 357 232
191 183 89 75 54 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 1879 1879
Size: 5624
SfoI GGC'GCC
Cuts at: 868 1093 1455 3056 3304 868
Size: 225 362 1601 248 3188
Fragments arranged by size:
3188 1601 362 248 225
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
SmaI CCC'GGG
Cuts at: 2646 2646
Size: 5624
SmlI C'TyrA_G
Cuts at: 1411 1720 2587 2779 3694 4408 4670 1411
Size: 309 867 192 915 714 262 2365
Fragments arranged by size:
2365 915 867 714 309 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5624
SphI G_CATG'C
Cuts at: 2677 3177 2677
Size: 500 5124
StuI AGG'CCT
Cuts at: 1983 2235 1983
Size: 252 5372
StyI C'CwwG_G
Cuts at: 107 345 717 759 896 1076 1424 1778 2138
Size: 238 372 42 137 180 348 354 360
Cuts at: 2138 2345 2758 3112 107
Size: 207 413 354 2619
Fragments arranged by size:
2619 413 372 360 354 354 348 238
207 180 137 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 847 1976 3207 3340 3799 4008 847
Size: 1129 1231 133 459 209 2463
Fragments arranged by size:
2463 1231 1129 459 209 133
TatI w'GTAC_w
Cuts at: 5139 5139
Size: 5624
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 349 381 511 543 756 788 2356 2388 5141
Size: 32 130 32 213 32 1568 32 2753
Cuts at: 5141 5173 349
Size: 32 800
Fragments arranged by size:
2753 1568 800 213 130 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1677 3421 1677
Size: 1744 3880
XbaI T'CTAG_A
Cuts at: 2655 2655
Size: 5624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 378 2337 3119 378
Size: 1959 782 2883
Fragments arranged by size:
2883 1959 782
XhoI C'TCGA_G
Cuts at: 1411 1411
Size: 5624
XmaI C'CCGG_G
Cuts at: 2644 2644
Size: 5624
XmnI GAAnn'nnTTC
Cuts at: 152 658 152
Size: 506 5118
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AloI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmI BspEI BsrGI BstZ17I
CspCI EcoRV FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PsrI SacII
SanDI SgrAI SnaBI SrfI SspI SwaI ZraI