Restriction map for
pF1KA0682
(Circular) (Six-base) MAPSORT of: pF1KA0682.seq Check: 2621 from: 1 to: 5993
pF1KA0682 5993 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 991 991
Size: 5993
AccI GT'mk_AC
Cuts at: 3031 3031
Size: 5993
Acc65I G'GTAC_C
Cuts at: 3009 3009
Size: 5993
AclI AA'CG_TT
Cuts at: 5833 5833
Size: 5993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 508 1821 2007 2120 2219 2365 2646 2965
Size: 282 1313 186 113 99 146 281 319
Cuts at: 2965 3818 4250 4997 5265 226
Size: 853 432 747 268 954
Fragments arranged by size:
1313 954 853 747 432 319 282 281
268 186 146 113 99
AflIII A'CryG_T
Cuts at: 1529 3345 4449 5426 1529
Size: 1816 1104 977 2096
Fragments arranged by size:
2096 1816 1104 977
AgeI A'CCGG_T
Cuts at: 4381 4381
Size: 5993
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 188 188
Size: 5993
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 487 519 610 642 487
Size: 32 91 32 5838
Fragments arranged by size:
5838 91 32 32
AlwNI CAG_nnn'CTG
Cuts at: 371 2179 2862 3298 4865 371
Size: 1808 683 436 1567 1499
Fragments arranged by size:
1808 1567 1499 683 436
ApaLI G'TGCA_C
Cuts at: 808 1232 4763 808
Size: 424 3531 2038
Fragments arranged by size:
3531 2038 424
ApoI r'AATT_y
Cuts at: 1042 2997 5346 5602 1042
Size: 1955 2349 256 1433
Fragments arranged by size:
2349 1955 1433 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5993
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5993
AvaI C'yCGr_G
Cuts at: 1273 3013 1273
Size: 1740 4253
BamHI G'GATC_C
Cuts at: 3018 3018
Size: 5993
BanI G'GyrC_C
Cuts at: 1050 1897 3009 3423 3671 3706 1050
Size: 847 1112 414 248 35 3337
Fragments arranged by size:
3337 1112 847 414 248 35
BanII G_rGCy'C
Cuts at: 581 1011 1400 2003 2079 2660 2897 3007 4037
Size: 430 389 603 76 581 237 110 1030
Cuts at: 4037 581
Size: 2537
Fragments arranged by size:
2537 1030 603 581 430 389 237 110
76
BbeI G_GCGC'C
Cuts at: 3427 3675 3427
Size: 248 5745
BbsI GAAGACnn'nnnn_
Cuts at: 2082 2689 2878 2082
Size: 607 189 5197
Fragments arranged by size:
5197 607 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 327 361 327
Size: 34 5959
BciVI GTATCCnnnnn_n'
Cuts at: 489 1102 1262 3891 4652 5993 489
Size: 613 160 2629 761 1341 489
Fragments arranged by size:
2629 1341 761 613 489 160
BclI T'GATC_A
Cuts at: 3513 3513
Size: 5993
BglI GCCn_nnn'nGGC
Cuts at: 277 553 1937 2017 277
Size: 276 1384 80 4253
Fragments arranged by size:
4253 1384 276 80
BglII A'GATC_T
Cuts at: 3508 5545 3508
Size: 2037 3956
BlpI GC'TnA_GC
Cuts at: 575 3105 575
Size: 2530 3463
Bme1580I G_kGCm'C
Cuts at: 812 1236 1578 3618 3711 4767 812
Size: 424 342 2040 93 1056 2038
Fragments arranged by size:
2040 2038 1056 424 342 93
BmgBI CAC'GTC
Cuts at: 2499 2499
Size: 5993
BmrI ACTGGGnnnn_n'
Cuts at: 853 2720 3205 3286 3621 5765 853
Size: 1867 485 81 335 2144 1081
Fragments arranged by size:
2144 1867 1081 485 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 864 1106 4360 864
Size: 242 3254 2497
Fragments arranged by size:
3254 2497 242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 416 499 1381 2017 2026 2063 2079 2089
Size: 23 83 882 636 9 37 16 10
Cuts at: 2089 2473 2848 2956 393
Size: 384 375 108 3430
Fragments arranged by size:
3430 882 636 384 375 108 83 37
23 16 10 9
Bpu10I CC'TnA_GC
Cuts at: 1466 1466
Size: 5993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2464 3169 4048 4540 4838 2464
Size: 705 879 492 298 3619
Fragments arranged by size:
3619 879 705 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 918 1729 1915 5697 918
Size: 811 186 3782 1214
Fragments arranged by size:
3782 1214 811 186
BsaAI yAC'GTr
Cuts at: 1530 3346 1530
Size: 1816 4177
BsaBI GATnn'nnATC
Cuts at: 6 2204 3507 5544 6
Size: 2198 1303 2037 455
Fragments arranged by size:
2198 2037 1303 455
BsaHI Gr'CG_yC
Cuts at: 2087 3424 3672 4374 5870 2087
Size: 1337 248 702 1496 2210
Fragments arranged by size:
2210 1496 1337 702 248
BsaWI w'CCGG_w
Cuts at: 930 1309 3182 3703 4381 4655 4802 5213 930
Size: 379 1873 521 678 274 147 411 1710
Fragments arranged by size:
1873 1710 678 521 411 379 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 502 532 610 640 502
Size: 30 78 30 5855
Fragments arranged by size:
5855 78 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 664 667 1303 1306 1366 2266 2269 2272
Size: 498 3 636 3 60 900 3 3
Cuts at: 2272 2983 166
Size: 711 3176
Fragments arranged by size:
3176 900 711 636 498 60 3 3
3 3
BseYI C'CCAG_C
Cuts at: 760 913 1778 2157 3418 4176 4753 5249 760
Size: 153 865 379 1261 758 577 496 1504
Fragments arranged by size:
1504 1261 865 758 577 496 379 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 908 730
Size: 178 5815
BsiEI CG_ry'CG
Cuts at: 104 1910 1932 2879 3535 3581 4789 104
Size: 1806 22 947 656 46 1208 1308
Fragments arranged by size:
1806 1308 1208 947 656 46 22
BsiHKAI G_wGCw'C
Cuts at: 812 1236 1400 1472 2420 2660 3007 3785 3975
Size: 424 164 72 948 240 347 778 190
Cuts at: 3975 4767 812
Size: 792 2038
Fragments arranged by size:
2038 948 792 778 424 347 240 190
164 72
BsmI GAATG_Cn'
Cuts at: 1444 1677 1444
Size: 233 5760
Bsp1286I G_dGCh'C
Cuts at: 581 812 1011 1236 1400 1472 1578 2003 2079
Size: 231 199 225 164 72 106 425 76
Cuts at: 2079 2420 2660 2897 3007 3618 3711 3785 3975
Size: 341 240 237 110 611 93 74 190
Cuts at: 3975 4037 4767 581
Size: 62 730 1807
Fragments arranged by size:
1807 730 611 425 341 240 237 231
225 199 190 164 110 106 93 76
74 72 62
BspEI T'CCGG_A
Cuts at: 1309 5213 1309
Size: 3904 2089
BspHI T'CATG_A
Cuts at: 5169 5169
Size: 5993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 460 991 1340 1494 3043 3257 3559 3940 460
Size: 531 349 154 1549 214 302 381 2513
Fragments arranged by size:
2513 1549 531 381 349 302 214 154
BsrBI CCG'CTC
Cuts at: 50 2881 4285 4339 5823 5988 50
Size: 2831 1404 54 1484 165 55
Fragments arranged by size:
2831 1484 1404 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1137 3905 1137
Size: 2768 3225
BsrFI r'CCGG_y
Cuts at: 600 3991 4172 4381 600
Size: 3391 181 209 2212
Fragments arranged by size:
3391 2212 209 181
BssSI C'ACGA_G
Cuts at: 1732 2890 4264 4622 1732
Size: 1158 1374 358 3103
Fragments arranged by size:
3103 1374 1158 358
BstAPI GCAn_nnn'nTGC
Cuts at: 906 2862 3370 906
Size: 1956 508 3529
Fragments arranged by size:
3529 1956 508
BstBI TT'CG_AA
Cuts at: 1074 4354 1074
Size: 3280 2713
BstEII G'GTnAC_C
Cuts at: 2847 2847
Size: 5993
BstXI CCAn_nnnn'nTGG
Cuts at: 1578 2610 1578
Size: 1032 4961
BstYI r'GATC_y
Cuts at: 1 1356 2528 2606 2648 2930 3018 3485 3508
Size: 1355 1172 78 42 282 88 467 23
Cuts at: 3508 3843 4089 5101 5187 5199 5545 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1355 1172 1012 467 449 346 335 282
246 88 86 78 42 23 12
BtgI C'CryG_G
Cuts at: 1676 2144 2426 1676
Size: 468 282 5243
Fragments arranged by size:
5243 468 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1674 3943 4124 4287 1674
Size: 2269 181 163 3380
Fragments arranged by size:
3380 2269 181 163
BtsI GCAGTG_nn'
Cuts at: 1976 2958 3236 1976
Size: 982 278 4733
Fragments arranged by size:
4733 982 278
DraI TTT'AAA
Cuts at: 2992 2992
Size: 5993
DraIII CAC_nnn'GTG
Cuts at: 993 1476 2502 993
Size: 483 1026 4484
Fragments arranged by size:
4484 1026 483
DrdI GACnn_nn'nnGTC
Cuts at: 1819 3699 4557 1819
Size: 1880 858 3255
Fragments arranged by size:
3255 1880 858
EaeI y'GGCC_r
Cuts at: 356 602 1067 1301 1679 1751 1907 1929 2508
Size: 246 465 234 378 72 156 22 579
Cuts at: 2508 2876 3578 3752 4143 4170 5515 356
Size: 368 702 174 391 27 1345 834
Fragments arranged by size:
1345 834 702 579 465 391 378 368
246 234 174 156 72 27 22
EagI C'GGCC_G
Cuts at: 1907 1929 2876 3578 1907
Size: 22 947 702 4322
Fragments arranged by size:
4322 947 702 22
EarI CTCTTCn'nnn_
Cuts at: 260 586 674 777 779 833 1130 1280 1375
Size: 326 88 103 2 54 297 150 95
Cuts at: 1375 2238 2243 2258 2273 2330 2371 2665 2764
Size: 863 5 15 15 57 41 294 99
Cuts at: 2764 4016 4226 260
Size: 1252 210 2027
Fragments arranged by size:
2027 1252 863 326 297 294 210 150
103 99 95 88 57 54 41 15
15 5 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 840 1985 2680 2829 2883 4201 4511 4657 5803
Size: 1145 695 149 54 1318 310 146 1146
Cuts at: 5803 840
Size: 1030
Fragments arranged by size:
1318 1146 1145 1030 695 310 149 146
54
EcoICRI GAG'CTC
Cuts at: 1398 2658 3005 1398
Size: 1260 347 4386
Fragments arranged by size:
4386 1260 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 393 416 499 508 1381 1821 2007 2017
Size: 167 23 83 9 873 440 186 10
Cuts at: 2017 2026 2063 2079 2089 2120 2219 2365 2473
Size: 9 37 16 10 31 99 146 108
Cuts at: 2473 2646 2848 2956 2965 3818 4250 4997 5265
Size: 173 202 108 9 853 432 747 268
Cuts at: 5265 226
Size: 954
Fragments arranged by size:
954 873 853 747 440 432 268 202
186 173 167 146 108 108 99 83
37 31 23 16 10 10 9 9
9
EcoO109I rG'GnC_Cy
Cuts at: 351 669 1296 2154 2859 3132 351
Size: 318 627 858 705 273 3212
Fragments arranged by size:
3212 858 705 627 318 273
EcoRI G'AATT_C
Cuts at: 1042 2997 1042
Size: 1955 4038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2793 4012 2793
Size: 1219 4774
FspI TGC'GCA
Cuts at: 3774 3774
Size: 5993
HaeII r_GCGC'y
Cuts at: 1188 3427 3675 4697 5676 1188
Size: 2239 248 1022 979 1505
Fragments arranged by size:
2239 1505 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 2550 2582 2865 2897 3835 3865 3867
Size: 32 2016 32 283 32 938 30 2
Cuts at: 3867 3897 502
Size: 30 2598
Fragments arranged by size:
2598 2016 938 283 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 475 3032 475
Size: 2557 3436
KasI G'GCGC_C
Cuts at: 3423 3671 3423
Size: 248 5745
KpnI G_GTAC'C
Cuts at: 3013 3013
Size: 5993
MluI A'CGCG_T
Cuts at: 5426 5426
Size: 5993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 704 2233 2903 4664 4848 704
Size: 1529 670 1761 184 1849
Fragments arranged by size:
1849 1761 1529 670 184
MscI TGG'CCA
Cuts at: 1069 3754 1069
Size: 2685 3308
MslI CAynn'nnrTG
Cuts at: 815 1117 1195 1456 3982 4109 815
Size: 302 78 261 2526 127 2699
Fragments arranged by size:
2699 2526 302 261 127 78
MspA1I CmG'CkG
Cuts at: 168 706 913 2943 2985 3104 3418 3778 4791
Size: 538 207 2030 42 119 314 360 1013
Cuts at: 4791 5036 168
Size: 245 1125
Fragments arranged by size:
2030 1125 1013 538 360 314 245 207
119 42
NaeI GCC'GGC
Cuts at: 602 4174 602
Size: 3572 2421
NarI GG'CG_CC
Cuts at: 3424 3672 3424
Size: 248 5745
NcoI C'CATG_G
Cuts at: 2144 2426 2144
Size: 282 5711
NgoMIV G'CCGG_C
Cuts at: 600 4172 600
Size: 3572 2421
NruI TCG'CGA
Cuts at: 114 114
Size: 5993
NspI r_CATG'y
Cuts at: 385 3046 3546 4453 385
Size: 2661 500 907 1925
Fragments arranged by size:
2661 1925 907 500
PasI CC'CwG_GG
Cuts at: 1578 1578
Size: 5993
PciI A'CATG_T
Cuts at: 4449 4449
Size: 5993
PflMI CCAn_nnn'nTGG
Cuts at: 371 1256 2517 371
Size: 885 1261 3847
Fragments arranged by size:
3847 1261 885
PfoI T'CCnGG_A
Cuts at: 316 506 1787 1994 2933 316
Size: 190 1281 207 939 3376
Fragments arranged by size:
3376 1281 939 207 190
PmeI GTTT'AAAC
Cuts at: 2992 2992
Size: 5993
PmlI CAC'GTG
Cuts at: 1530 1530
Size: 5993
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 610 642 5132 5164 610
Size: 32 4490 32 1439
Fragments arranged by size:
4490 1439 32 32
PpuMI rG'GwC_Cy
Cuts at: 351 669 1296 2154 351
Size: 318 627 858 4190
Fragments arranged by size:
4190 858 627 318
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1321 1353 1321
Size: 32 5961
PstI C_TGCA'G
Cuts at: 202 869 1851 1919 2858 3040 3725 202
Size: 667 982 68 939 182 685 2470
Fragments arranged by size:
2470 982 939 685 667 182 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5993
PvuII CAG'CTG
Cuts at: 168 706 913 2943 2985 3418 3778 168
Size: 538 207 2030 42 433 360 2383
Fragments arranged by size:
2383 2030 538 433 360 207 42
RsrII CG'GwC_CG
Cuts at: 2688 4188 2688
Size: 1500 4493
SacI G_AGCT'C
Cuts at: 1400 2660 3007 1400
Size: 1260 347 4386
Fragments arranged by size:
4386 1260 347
SalI G'TCGA_C
Cuts at: 3030 3030
Size: 5993
SanDI GG'GwC_CC
Cuts at: 351 351
Size: 5993
SapI GCTCTTCn'nnn_
Cuts at: 586 1375 2371 2665 4016 4226 586
Size: 789 996 294 1351 210 2353
Fragments arranged by size:
2353 1351 996 789 294 210
SbfI CC_TGCA'GG
Cuts at: 869 2858 3040 869
Size: 1989 182 3822
Fragments arranged by size:
3822 1989 182
ScaI AGT'ACT
Cuts at: 5510 5510
Size: 5993
SfcI C'TryA_G
Cuts at: 33 198 865 1847 1915 2854 3036 3721 4714
Size: 165 667 982 68 939 182 685 993
Cuts at: 4714 4905 33
Size: 191 1121
Fragments arranged by size:
1121 993 982 939 685 667 191 182
165 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 2017 2017
Size: 5993
SfoI GGC'GCC
Cuts at: 3425 3673 3425
Size: 248 5745
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5993
SmaI CCC'GGG
Cuts at: 3015 3015
Size: 5993
SmlI C'TyrA_G
Cuts at: 2479 3148 4063 4555 4817 2479
Size: 669 915 492 262 3655
Fragments arranged by size:
3655 915 669 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5993
SphI G_CATG'C
Cuts at: 3046 3546 3046
Size: 500 5493
StuI AGG'CCT
Cuts at: 1483 2020 1483
Size: 537 5456
StyI C'CwwG_G
Cuts at: 1226 1433 1739 2144 2426 3127 3481 1226
Size: 207 306 405 282 701 354 3738
Fragments arranged by size:
3738 701 405 354 306 282 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1168 2815 3576 3709 4168 4377 1168
Size: 1647 761 133 459 209 2784
Fragments arranged by size:
2784 1647 761 459 209 133
TatI w'GTAC_w
Cuts at: 1172 1700 2296 5508 1172
Size: 528 596 3212 1657
Fragments arranged by size:
3212 1657 596 528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5510 5542 5510
Size: 32 5961
Tth111I GACn'n_nGTC
Cuts at: 3790 3790
Size: 5993
XbaI T'CTAG_A
Cuts at: 3024 3024
Size: 5993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1746 1983 3488 1746
Size: 237 1505 4251
Fragments arranged by size:
4251 1505 237
XmaI C'CCGG_G
Cuts at: 3013 3013
Size: 5993
XmnI GAAnn'nnTTC
Cuts at: 289 1369 289
Size: 1080 4913
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PshAI PsiI PspOMI PspXI SacII SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI