Restriction map for
pF1KA0678
(Circular) (Six-base) MAPSORT of: pF1KA0678.seq Check: 863 from: 1 to: 9842
pF1KA0678 9842 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 618 2010 3606 5141 6803 6880 618
Size: 1392 1596 1535 1662 77 3580
Fragments arranged by size:
3580 1662 1596 1535 1392 77
Acc65I G'GTAC_C
Cuts at: 6858 6858
Size: 9842
AclI AA'CG_TT
Cuts at: 9682 9682
Size: 9842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1769 3151 3647 4065 4310 4439 4910 5864 6020
Size: 1382 496 418 245 129 471 954 156
Cuts at: 6020 6195 6641 7667 8099 8452 9114 1769
Size: 175 446 1026 432 353 662 2497
Fragments arranged by size:
2497 1382 1026 954 662 496 471 446
432 418 353 245 175 156 129
AflII C'TTAA_G
Cuts at: 1578 3184 1578
Size: 1606 8236
AflIII A'CryG_T
Cuts at: 886 3254 4130 4511 7194 8994 9275 886
Size: 2368 876 381 2683 1800 281 1453
Fragments arranged by size:
2683 2368 1800 1453 876 381 281
AhdI GACnn_n'nnGTC
Cuts at: 3214 3214
Size: 9842
AleI CACnn'nnGTG
Cuts at: 470 470
Size: 9842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 668 1931 668
Size: 1263 8579
AlwNI CAG_nnn'CTG
Cuts at: 1225 3134 3378 5404 6421 6736 7147 8585 1225
Size: 1909 244 2026 1017 315 411 1438 2482
Fragments arranged by size:
2482 2026 1909 1438 1017 411 315 244
ApaLI G'TGCA_C
Cuts at: 2817 8680 2817
Size: 5863 3979
ApoI r'AATT_y
Cuts at: 358 748 997 1648 2185 2245 2436 2662 3691
Size: 390 249 651 537 60 191 226 1029
Cuts at: 3691 4159 4733 5068 5134 5800 6109 6846 9195
Size: 468 574 335 66 666 309 737 2349
Cuts at: 9195 9451 358
Size: 256 749
Fragments arranged by size:
2349 1029 749 737 666 651 574 537
468 390 335 309 256 249 226 191
66 60
AseI AT'TA_AT
Cuts at: 20 527 6446 20
Size: 507 5919 3416
Fragments arranged by size:
5919 3416 507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9842
AvaI C'yCGr_G
Cuts at: 6862 6862
Size: 9842
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4081 4114 4524 4557 4081
Size: 33 410 33 9366
Fragments arranged by size:
9366 410 33 33
BamHI G'GATC_C
Cuts at: 6867 6867
Size: 9842
BanI G'GyrC_C
Cuts at: 1678 1930 3731 6858 7272 7520 7555 1678
Size: 252 1801 3127 414 248 35 3965
Fragments arranged by size:
3965 3127 1801 414 252 248 35
BanII G_rGCy'C
Cuts at: 3141 4427 5727 6146 6856 7886 3141
Size: 1286 1300 419 710 1030 5097
Fragments arranged by size:
5097 1300 1286 1030 710 419
BbeI G_GCGC'C
Cuts at: 3735 7276 7524 3735
Size: 3541 248 6053
Fragments arranged by size:
6053 3541 248
BbsI GAAGACnn'nnnn_
Cuts at: 2358 3775 6048 2358
Size: 1417 2273 6152
Fragments arranged by size:
6152 2273 1417
BbvCI CC'TCA_GC
Cuts at: 6224 6224
Size: 9842
BciVI GTATCCnnnnn_n'
Cuts at: 4282 4525 6715 7740 8796 9842 4282
Size: 243 2190 1025 1056 1046 4282
Fragments arranged by size:
4282 2190 1056 1046 1025 243
BclI T'GATC_A
Cuts at: 1316 3260 5148 7362 1316
Size: 1944 1888 2214 3796
Fragments arranged by size:
3796 2214 1944 1888
BfrBI ATG'CAT
Cuts at: 1563 2142 5417 1563
Size: 579 3275 5988
Fragments arranged by size:
5988 3275 579
BglI GCCn_nnn'nGGC
Cuts at: 6574 6574
Size: 9842
BglII A'GATC_T
Cuts at: 588 2109 7357 9394 588
Size: 1521 5248 2037 1036
Fragments arranged by size:
5248 2037 1521 1036
BlpI GC'TnA_GC
Cuts at: 4932 6954 4932
Size: 2022 7820
Bme1580I G_kGCm'C
Cuts at: 1548 1683 2821 3617 5426 7467 7560 8684 1548
Size: 135 1138 796 1809 2041 93 1124 2706
Fragments arranged by size:
2706 2041 1809 1138 1124 796 135 93
BmrI ACTGGGnnnn_n'
Cuts at: 7054 7135 7470 9614 7054
Size: 81 335 2144 7282
Fragments arranged by size:
7282 2144 335 81
BmtI G_CTAG'C
Cuts at: 4384 4384
Size: 9842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8209 8209
Size: 9842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 491 1663 2304 2987 3686 3735 4889 5239
Size: 2 1172 641 683 699 49 1154 350
Cuts at: 5239 6684 6758 489
Size: 1445 74 3573
Fragments arranged by size:
3573 1445 1172 1154 699 683 641 350
74 49 2
Bpu10I CC'TnA_GC
Cuts at: 6224 6224
Size: 9842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 1402 3125 4019 6081 6580 7018 7897 8611
Size: 940 1723 894 2062 499 438 879 714
Cuts at: 8611 8909 462
Size: 298 1395
Fragments arranged by size:
2062 1723 1395 940 894 879 714 499
438 298
BsaI GGTCTCn'nnnn_
Cuts at: 1294 6370 9546 1294
Size: 5076 3176 1590
Fragments arranged by size:
5076 3176 1590
BsaAI yAC'GTr
Cuts at: 7195 7195
Size: 9842
BsaBI GATnn'nnATC
Cuts at: 6 2132 4112 7356 9393 6
Size: 2126 1980 3244 2037 455
Fragments arranged by size:
3244 2126 2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 3732 7273 7521 8223 9719 3732
Size: 3541 248 702 1496 3855
Fragments arranged by size:
3855 3541 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3902 7031 7552 8230 8641 8788 9062 3902
Size: 3129 521 678 411 147 274 4682
Fragments arranged by size:
4682 3129 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 6617 6647 6617
Size: 30 9812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1419 6065 1419
Size: 4646 5196
BseYI C'CCAG_C
Cuts at: 1849 3134 3179 6561 7267 8025 8690 9098 1849
Size: 1285 45 3382 706 758 665 408 2593
Fragments arranged by size:
3382 2593 1285 758 706 665 408 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 5494 5494
Size: 9842
BsiEI CG_ry'CG
Cuts at: 104 7384 7430 8660 104
Size: 7280 46 1230 1286
Fragments arranged by size:
7280 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 374 1731 2821 3804 4427 5954 6146 6856 7634
Size: 1357 1090 983 623 1527 192 710 778
Cuts at: 7634 7824 8684 374
Size: 190 860 1532
Fragments arranged by size:
1532 1527 1357 1090 983 860 778 710
623 192 190
BsmI GAATG_Cn'
Cuts at: 4579 4793 5419 4579
Size: 214 626 9002
Fragments arranged by size:
9002 626 214
Bsp1286I G_dGCh'C
Cuts at: 374 1548 1683 1731 2821 3141 3617 3804 4427
Size: 1174 135 48 1090 320 476 187 623
Cuts at: 4427 5426 5727 5954 6146 6856 7467 7560 7634
Size: 999 301 227 192 710 611 93 74
Cuts at: 7634 7824 7886 8684 374
Size: 190 62 798 1532
Fragments arranged by size:
1532 1174 1090 999 798 710 623 611
476 320 301 227 192 190 187 135
93 74 62 48
BspHI T'CATG_A
Cuts at: 3081 3491 5874 8274 3081
Size: 410 2383 2400 4649
Fragments arranged by size:
4649 2400 2383 410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 6751 6797 6892 7106 7408 7789 6751
Size: 46 95 214 302 381 8804
Fragments arranged by size:
8804 381 302 214 95 46
BsrBI CCG'CTC
Cuts at: 50 8134 8188 9672 9837 50
Size: 8084 54 1484 165 55
Fragments arranged by size:
8084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 721 2174 2300 2991 3638 5581 5663 6284 6608
Size: 1453 126 691 647 1943 82 621 324
Cuts at: 6608 7754 721
Size: 1146 2809
Fragments arranged by size:
2809 1943 1453 1146 691 647 621 324
126 82
BsrFI r'CCGG_y
Cuts at: 7840 8021 7840
Size: 181 9661
BsrGI T'GTAC_A
Cuts at: 3497 5853 3497
Size: 2356 7486
BssSI C'ACGA_G
Cuts at: 2447 8113 8821 2447
Size: 5666 708 3468
Fragments arranged by size:
5666 3468 708
BstAPI GCAn_nnn'nTGC
Cuts at: 5239 5668 7219 5239
Size: 429 1551 7862
Fragments arranged by size:
7862 1551 429
BstBI TT'CG_AA
Cuts at: 2343 5784 8203 2343
Size: 3441 2419 3982
Fragments arranged by size:
3982 3441 2419
BstXI CCAn_nnnn'nTGG
Cuts at: 5565 5565
Size: 9842
BstYI r'GATC_y
Cuts at: 1 132 588 2109 2265 2382 2892 4200 4614
Size: 131 456 1521 156 117 510 1308 414
Cuts at: 4614 6065 6867 7334 7357 7692 7938 8244 8256
Size: 1451 802 467 23 335 246 306 12
Cuts at: 8256 8342 9394 1
Size: 86 1052 449
Fragments arranged by size:
1521 1451 1308 1052 802 510 467 456
449 414 335 306 246 156 131 117
86 23 12
BstZ17I GTA'TAC
Cuts at: 619 3607 619
Size: 2988 6854
Bsu36I CC'TnA_GG
Cuts at: 1183 5978 1183
Size: 4795 5047
BtgI C'CryG_G
Cuts at: 1829 6362 1829
Size: 4533 5309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1146 1972 2668 7792 7973 8136 1146
Size: 826 696 5124 181 163 2852
Fragments arranged by size:
5124 2852 826 696 181 163
BtsI GCAGTG_nn'
Cuts at: 339 2974 3726 7085 339
Size: 2635 752 3359 3096
Fragments arranged by size:
3359 3096 2635 752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3079 3114 4169 4204 3079
Size: 35 1055 35 8717
Fragments arranged by size:
8717 1055 35 35
DraI TTT'AAA
Cuts at: 356 1809 5958 6841 356
Size: 1453 4149 883 3357
Fragments arranged by size:
4149 3357 1453 883
DraIII CAC_nnn'GTG
Cuts at: 3269 3269
Size: 9842
DrdI GACnn_nn'nnGTC
Cuts at: 7548 8892 7548
Size: 1344 8498
EaeI y'GGCC_r
Cuts at: 3916 5294 7427 7601 7992 8019 9364 3916
Size: 1378 2133 174 391 27 1345 4394
Fragments arranged by size:
4394 2133 1378 1345 391 174 27
EagI C'GGCC_G
Cuts at: 7427 7427
Size: 9842
EarI CTCTTCn'nnn_
Cuts at: 665 1297 1613 2392 2857 3566 4249 4415 4425
Size: 632 316 779 465 709 683 166 10
Cuts at: 4425 4778 5450 5576 6214 7865 8075 665
Size: 353 672 126 638 1651 210 2432
Fragments arranged by size:
2432 1651 779 709 683 672 638 632
465 353 316 210 166 126 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8050 8792 8938 9652 8050
Size: 742 146 714 8240
Fragments arranged by size:
8240 742 714 146
EcoICRI GAG'CTC
Cuts at: 4425 6144 6854 4425
Size: 1719 710 7413
Fragments arranged by size:
7413 1719 710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 489 491 1663 1769 2304 2987 3151 3647 3686
Size: 2 1172 106 535 683 164 496 39
Cuts at: 3686 3735 4065 4310 4439 4889 4910 5239 5864
Size: 49 330 245 129 450 21 329 625
Cuts at: 5864 6020 6195 6641 6684 6758 7667 8099 8452
Size: 156 175 446 43 74 909 432 353
Cuts at: 8452 9114 489
Size: 662 1217
Fragments arranged by size:
1217 1172 909 683 662 625 535 496
450 446 432 353 330 329 245 175
164 156 129 106 74 49 43 39
21 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2572 4723 2572
Size: 2151 7691
EcoO109I rG'GnC_Cy
Cuts at: 2773 6240 6733 6981 2773
Size: 3467 493 248 5634
Fragments arranged by size:
5634 3467 493 248
EcoRI G'AATT_C
Cuts at: 748 6846 748
Size: 6098 3744
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 204 1624 2284 2485 3970 7861 204
Size: 1420 660 201 1485 3891 2185
Fragments arranged by size:
3891 2185 1485 1420 660 201
FspI TGC'GCA
Cuts at: 7623 7623
Size: 9842
HaeII r_GCGC'y
Cuts at: 3735 7276 7524 8754 9525 3735
Size: 3541 248 1230 771 4052
Fragments arranged by size:
4052 3541 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 4571 4603 5427 5459 5823 5855 5905
Size: 32 1339 32 824 32 364 32 50
Cuts at: 5905 5937 7684 7714 7716 7746 3200
Size: 32 1747 30 2 30 5296
Fragments arranged by size:
5296 1747 1339 824 364 50 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4398 5529 6881 4398
Size: 1131 1352 7359
Fragments arranged by size:
7359 1352 1131
HindIII A'AGCT_T
Cuts at: 1601 3937 4073 4929 1601
Size: 2336 136 856 6514
Fragments arranged by size:
6514 2336 856 136
KasI G'GCGC_C
Cuts at: 3731 7272 7520 3731
Size: 3541 248 6053
Fragments arranged by size:
6053 3541 248
KpnI G_GTAC'C
Cuts at: 6862 6862
Size: 9842
MluI A'CGCG_T
Cuts at: 9275 9275
Size: 9842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 177 267 4939 5238 6027 8601 8785 177
Size: 90 4672 299 789 2574 184 1234
Fragments arranged by size:
4672 2574 1234 789 299 184 90
MscI TGG'CCA
Cuts at: 3918 5296 7603 3918
Size: 1378 2307 6157
Fragments arranged by size:
6157 2307 1378
MslI CAynn'nnrTG
Cuts at: 470 2803 2951 4594 5290 7831 7958 470
Size: 2333 148 1643 696 2541 127 2354
Fragments arranged by size:
2541 2354 2333 1643 696 148 127
MspA1I CmG'CkG
Cuts at: 2035 6228 6953 7267 7627 8411 8656 2035
Size: 4193 725 314 360 784 245 3221
Fragments arranged by size:
4193 3221 784 725 360 314 245
NaeI GCC'GGC
Cuts at: 8023 8023
Size: 9842
NarI GG'CG_CC
Cuts at: 3732 7273 7521 3732
Size: 3541 248 6053
Fragments arranged by size:
6053 3541 248
NcoI C'CATG_G
Cuts at: 1829 6362 1829
Size: 4533 5309
NdeI CA'TA_TG
Cuts at: 4155 4155
Size: 9842
NgoMIV G'CCGG_C
Cuts at: 8021 8021
Size: 9842
NheI G'CTAG_C
Cuts at: 4380 4380
Size: 9842
NruI TCG'CGA
Cuts at: 1463 1463
Size: 9842
NsiI A_TGCA'T
Cuts at: 1565 2144 5419 1565
Size: 579 3275 5988
Fragments arranged by size:
5988 3275 579
NspI r_CATG'y
Cuts at: 143 890 1152 1273 2146 3258 4134 4515 4944
Size: 747 262 121 873 1112 876 381 429
Cuts at: 4944 6895 7395 8998 143
Size: 1951 500 1603 987
Fragments arranged by size:
1951 1603 1112 987 876 873 747 500
429 381 262 121
PciI A'CATG_T
Cuts at: 886 3254 4130 4511 8994 886
Size: 2368 876 381 4483 1734
Fragments arranged by size:
4483 2368 1734 876 381
PflMI CCAn_nnn'nTGG
Cuts at: 3134 3134
Size: 9842
PfoI T'CCnGG_A
Cuts at: 507 2530 507
Size: 2023 7819
PmeI GTTT'AAAC
Cuts at: 6841 6841
Size: 9842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1375 1407 8288 8320 1375
Size: 32 6881 32 2897
Fragments arranged by size:
6881 2897 32 32
PpuMI rG'GwC_Cy
Cuts at: 2773 6240 6733 2773
Size: 3467 493 5882
Fragments arranged by size:
5882 3467 493
PshAI GACnn'nnGTC
Cuts at: 3339 3339
Size: 9842
PsiI TTA'TAA
Cuts at: 1003 4277 1003
Size: 3274 6568
PstI C_TGCA'G
Cuts at: 6889 7574 6889
Size: 685 9157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9842
PvuII CAG'CTG
Cuts at: 2035 6228 7267 7627 2035
Size: 4193 1039 360 4250
Fragments arranged by size:
4250 4193 1039 360
RsrII CG'GwC_CG
Cuts at: 8037 8037
Size: 9842
SacI G_AGCT'C
Cuts at: 4427 6146 6856 4427
Size: 1719 710 7413
Fragments arranged by size:
7413 1719 710
SalI G'TCGA_C
Cuts at: 6879 6879
Size: 9842
SapI GCTCTTCn'nnn_
Cuts at: 4415 5576 7865 8075 4415
Size: 1161 2289 210 6182
Fragments arranged by size:
6182 2289 1161 210
SbfI CC_TGCA'GG
Cuts at: 6889 6889
Size: 9842
ScaI AGT'ACT
Cuts at: 548 9359 548
Size: 8811 1031
SfcI C'TryA_G
Cuts at: 33 365 564 2966 4373 4548 4708 5142 6800
Size: 332 199 2402 1407 175 160 434 1658
Cuts at: 6800 6885 7570 8538 8729 33
Size: 85 685 968 191 1146
Fragments arranged by size:
2402 1658 1407 1146 968 685 434 332
199 191 175 160 85
SfoI GGC'GCC
Cuts at: 3733 7274 7522 3733
Size: 3541 248 6053
Fragments arranged by size:
6053 3541 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9842
SmaI CCC'GGG
Cuts at: 6864 6864
Size: 9842
SmlI C'TyrA_G
Cuts at: 477 1381 1578 3140 3184 4034 6060 6595 6997
Size: 904 197 1562 44 850 2026 535 402
Cuts at: 6997 7912 8626 8888 477
Size: 915 714 262 1431
Fragments arranged by size:
2026 1562 1431 915 904 850 714 535
402 262 197 44
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9842
SphI G_CATG'C
Cuts at: 1152 4944 6895 7395 1152
Size: 3792 1951 500 3599
Fragments arranged by size:
3792 3599 1951 500
SspI AAT'ATT
Cuts at: 573 760 921 1024 1248 2496 3870 5535 573
Size: 187 161 103 224 1248 1374 1665 4880
Fragments arranged by size:
4880 1665 1374 1248 224 187 161 103
StyI C'CwwG_G
Cuts at: 1829 4749 4872 6362 6822 6976 7330 1829
Size: 2920 123 1490 460 154 354 4341
Fragments arranged by size:
4341 2920 1490 460 354 154 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 7425 7558 8017 8226 7425
Size: 133 459 209 9041
Fragments arranged by size:
9041 459 209 133
TatI w'GTAC_w
Cuts at: 546 3497 4518 5635 5853 9357 546
Size: 2951 1021 1117 218 3504 1031
Fragments arranged by size:
3504 2951 1117 1031 1021 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1434 1466 3342 3374 4223 4255 4433 4465 5007
Size: 32 1876 32 849 32 178 32 542
Cuts at: 5007 5039 6617 6649 9359 9391 1434
Size: 32 1578 32 2710 32 1885
Fragments arranged by size:
2710 1885 1876 1578 849 542 178 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5524 7639 5524
Size: 2115 7727
XbaI T'CTAG_A
Cuts at: 6873 6873
Size: 9842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1666 2259 3710 5033 7337 1666
Size: 593 1451 1323 2304 4171
Fragments arranged by size:
4171 2304 1451 1323 593
XmaI C'CCGG_G
Cuts at: 6862 6862
Size: 9842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AloI ApaI AscI AvrII
BcgI BmgBI BsiWI BsmBI BspEI BssHII BstEII ClaI
EcoRV FseI FspAI HpaI MfeI NotI PacI PasI
PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI