Restriction map for
pF1KA0675
(Circular) (Six-base) MAPSORT of: pF1KA0675.seq Check: 7465 from: 1 to: 6737
pF1KA0675 6737 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1808 2205 2834 3775 1808
Size: 397 629 941 4770
Fragments arranged by size:
4770 941 629 397
Acc65I G'GTAC_C
Cuts at: 3753 3753
Size: 6737
AclI AA'CG_TT
Cuts at: 6577 6577
Size: 6737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4562 4994 5347 6009 4562
Size: 432 353 662 5290
Fragments arranged by size:
5290 662 432 353
AflIII A'CryG_T
Cuts at: 3655 4089 5889 6170 3655
Size: 434 1800 281 4222
Fragments arranged by size:
4222 1800 434 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2945 2945
Size: 6737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2245 2277 2245
Size: 32 6705
AlwNI CAG_nnn'CTG
Cuts at: 577 3148 4042 5480 577
Size: 2571 894 1438 1834
Fragments arranged by size:
2571 1834 1438 894
ApaLI G'TGCA_C
Cuts at: 5575 5575
Size: 6737
ApoI r'AATT_y
Cuts at: 127 586 623 709 974 996 1112 1158 1234
Size: 459 37 86 265 22 116 46 76
Cuts at: 1234 3610 3694 3741 6090 6346 127
Size: 2376 84 47 2349 256 518
Fragments arranged by size:
2376 2349 518 459 265 256 116 86
84 76 47 46 37 22
AseI AT'TA_AT
Cuts at: 20 290 494 2006 20
Size: 270 204 1512 4751
Fragments arranged by size:
4751 1512 270 204
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6737
AvaI C'yCGr_G
Cuts at: 3075 3757 3075
Size: 682 6055
AvrII C'CTAG_G
Cuts at: 1812 1812
Size: 6737
BamHI G'GATC_C
Cuts at: 3762 3762
Size: 6737
BanI G'GyrC_C
Cuts at: 3753 4167 4415 4450 3753
Size: 414 248 35 6040
Fragments arranged by size:
6040 414 248 35
BanII G_rGCy'C
Cuts at: 2311 3111 3138 3751 4781 2311
Size: 800 27 613 1030 4267
Fragments arranged by size:
4267 1030 800 613 27
BbeI G_GCGC'C
Cuts at: 4171 4419 4171
Size: 248 6489
BbsI GAAGACnn'nnnn_
Cuts at: 2104 2138 2261 3015 2104
Size: 34 123 754 5826
Fragments arranged by size:
5826 754 123 34
BciVI GTATCCnnnnn_n'
Cuts at: 1016 4635 5691 6737 1016
Size: 3619 1056 1046 1016
Fragments arranged by size:
3619 1056 1046 1016
BclI T'GATC_A
Cuts at: 1020 4257 1020
Size: 3237 3500
BglI GCCn_nnn'nGGC
Cuts at: 2854 2854
Size: 6737
BglII A'GATC_T
Cuts at: 804 2058 2599 4252 6289 804
Size: 1254 541 1653 2037 1252
Fragments arranged by size:
2037 1653 1254 1252 541
BlpI GC'TnA_GC
Cuts at: 2310 3849 2310
Size: 1539 5198
Bme1580I G_kGCm'C
Cuts at: 4362 4455 5579 4362
Size: 93 1124 5520
Fragments arranged by size:
5520 1124 93
BmgBI CAC'GTC
Cuts at: 661 661
Size: 6737
BmrI ACTGGGnnnn_n'
Cuts at: 2024 2969 3164 3345 3949 4030 4365 6509 2024
Size: 945 195 181 604 81 335 2144 2252
Fragments arranged by size:
2252 2144 945 604 335 195 181 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 556 1797 2325 5104 556
Size: 1241 528 2779 2189
Fragments arranged by size:
2779 2189 1241 528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2933 3489 3560 2047
Size: 886 556 71 5224
Fragments arranged by size:
5224 886 556 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2955 3913 4792 5506 5804 2955
Size: 958 879 714 298 3888
Fragments arranged by size:
3888 958 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1672 6441 1672
Size: 4769 1968
BsaAI yAC'GTr
Cuts at: 2652 4090 2652
Size: 1438 5299
BsaBI GATnn'nnATC
Cuts at: 6 607 1793 4251 6288 6
Size: 601 1186 2458 2037 455
Fragments arranged by size:
2458 2037 1186 601 455
BsaHI Gr'CG_yC
Cuts at: 4168 4416 5118 6614 4168
Size: 248 702 1496 4291
Fragments arranged by size:
4291 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3926 4447 5125 5536 5683 5957 3926
Size: 521 678 411 147 274 4706
Fragments arranged by size:
4706 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2931 2961 3146 3176 2931
Size: 30 185 30 6492
Fragments arranged by size:
6492 185 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1352 2954 1352
Size: 1602 5135
BseYI C'CCAG_C
Cuts at: 3449 3709 4162 4920 5585 5993 3449
Size: 260 453 758 665 408 4193
Fragments arranged by size:
4193 758 665 453 408 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 963 1535 3194 3687 963
Size: 572 1659 493 4013
Fragments arranged by size:
4013 1659 572 493
BsiEI CG_ry'CG
Cuts at: 104 4279 4325 5555 104
Size: 4175 46 1230 1286
Fragments arranged by size:
4175 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 681 3751 4529 4719 5579 681
Size: 3070 778 190 860 1839
Fragments arranged by size:
3070 1839 860 778 190
BsmI GAATG_Cn'
Cuts at: 2850 2878 2850
Size: 28 6709
BsmBI CGTCTCn'nnnn_
Cuts at: 3090 3149 3090
Size: 59 6678
Bsp1286I G_dGCh'C
Cuts at: 681 2311 3111 3138 3751 4362 4455 4529 4719
Size: 1630 800 27 613 611 93 74 190
Cuts at: 4719 4781 5579 681
Size: 62 798 1839
Fragments arranged by size:
1839 1630 800 798 613 611 190 93
74 62 27
BspHI T'CATG_A
Cuts at: 3200 3561 5169 3200
Size: 361 1608 4768
Fragments arranged by size:
4768 1608 361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3166 3787 4001 4303 4684 3166
Size: 621 214 302 381 5219
Fragments arranged by size:
5219 621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5029 5083 6567 6732 50
Size: 4979 54 1484 165 55
Fragments arranged by size:
4979 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1093 2255 4649 1093
Size: 1162 2394 3181
Fragments arranged by size:
3181 2394 1162
BsrFI r'CCGG_y
Cuts at: 3713 4735 4916 3713
Size: 1022 181 5534
Fragments arranged by size:
5534 1022 181
BsrGI T'GTAC_A
Cuts at: 1060 1060
Size: 6737
BssSI C'ACGA_G
Cuts at: 5008 5716 5008
Size: 708 6029
BstAPI GCAn_nnn'nTGC
Cuts at: 4114 4114
Size: 6737
BstBI TT'CG_AA
Cuts at: 5098 5098
Size: 6737
BstEII G'GTnAC_C
Cuts at: 3703 3703
Size: 6737
BstYI r'GATC_y
Cuts at: 1 765 804 2058 2599 3762 4229 4252 4587
Size: 764 39 1254 541 1163 467 23 335
Cuts at: 4587 4833 5139 5151 5237 6289 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1254 1163 1052 764 541 467 449 335
306 246 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 2884 2884
Size: 6737
BtgI C'CryG_G
Cuts at: 107 3634 107
Size: 3527 3210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 687 4687 4868 5031 687
Size: 4000 181 163 2393
Fragments arranged by size:
4000 2393 181 163
BtsI GCAGTG_nn'
Cuts at: 3980 3980
Size: 6737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 290 325 290
Size: 35 6702
DraI TTT'AAA
Cuts at: 801 1001 1232 1538 3736 801
Size: 200 231 306 2198 3802
Fragments arranged by size:
3802 2198 306 231 200
DrdI GACnn_nn'nnGTC
Cuts at: 4443 5787 4443
Size: 1344 5393
EaeI y'GGCC_r
Cuts at: 3096 4322 4496 4887 4914 6259 3096
Size: 1226 174 391 27 1345 3574
Fragments arranged by size:
3574 1345 1226 391 174 27
EagI C'GGCC_G
Cuts at: 4322 4322
Size: 6737
EarI CTCTTCn'nnn_
Cuts at: 1907 2156 3524 3536 4760 4970 1907
Size: 249 1368 12 1224 210 3674
Fragments arranged by size:
3674 1368 1224 249 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4945 5687 5833 6547 4945
Size: 742 146 714 5135
Fragments arranged by size:
5135 742 714 146
EcoICRI GAG'CTC
Cuts at: 3749 3749
Size: 6737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2933 3489 3560 4562 4994 5347 6009 2047
Size: 886 556 71 1002 432 353 662 2775
Fragments arranged by size:
2775 1002 886 662 556 432 353 71
EcoO109I rG'GnC_Cy
Cuts at: 2018 3490 3876 2018
Size: 1472 386 4879
Fragments arranged by size:
4879 1472 386
EcoRI G'AATT_C
Cuts at: 3694 3741 3694
Size: 47 6690
EcoRV GAT'ATC
Cuts at: 1187 1638 1187
Size: 451 6286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 348 469 826 1399 2602 4756 348
Size: 121 357 573 1203 2154 2329
Fragments arranged by size:
2329 2154 1203 573 357 121
FspI TGC'GCA
Cuts at: 4518 4518
Size: 6737
HaeII r_GCGC'y
Cuts at: 3148 4171 4419 5649 6420 3148
Size: 1023 248 1230 771 3465
Fragments arranged by size:
3465 1230 1023 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1208 1240 1419 1451 1650 1682 2005 2037 3556
Size: 32 179 32 199 32 323 32 1519
Cuts at: 3556 3588 4579 4609 4611 4641 1208
Size: 32 991 30 2 30 3304
Fragments arranged by size:
3304 1519 991 323 199 179 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 205 252 3776 205
Size: 47 3524 3166
Fragments arranged by size:
3524 3166 47
HindIII A'AGCT_T
Cuts at: 720 1267 720
Size: 547 6190
HpaI GTT'AAC
Cuts at: 252 252
Size: 6737
KasI G'GCGC_C
Cuts at: 4167 4415 4167
Size: 248 6489
KpnI G_GTAC'C
Cuts at: 3757 3757
Size: 6737
MluI A'CGCG_T
Cuts at: 6170 6170
Size: 6737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2821 3685 5496 5680 2821
Size: 864 1811 184 3878
Fragments arranged by size:
3878 1811 864 184
MscI TGG'CCA
Cuts at: 3098 4498 3098
Size: 1400 5337
MslI CAynn'nnrTG
Cuts at: 1394 1538 4726 4853 1394
Size: 144 3188 127 3278
Fragments arranged by size:
3278 3188 144 127
MspA1I CmG'CkG
Cuts at: 2314 3848 4162 4522 5306 5551 2314
Size: 1534 314 360 784 245 3500
Fragments arranged by size:
3500 1534 784 360 314 245
NaeI GCC'GGC
Cuts at: 3715 4918 3715
Size: 1203 5534
NarI GG'CG_CC
Cuts at: 4168 4416 4168
Size: 248 6489
NcoI C'CATG_G
Cuts at: 107 3634 107
Size: 3527 3210
NdeI CA'TA_TG
Cuts at: 2145 2145
Size: 6737
NgoMIV G'CCGG_C
Cuts at: 3713 4916 3713
Size: 1203 5534
NspI r_CATG'y
Cuts at: 3659 3790 4290 5893 3659
Size: 131 500 1603 4503
Fragments arranged by size:
4503 1603 500 131
PciI A'CATG_T
Cuts at: 3655 5889 3655
Size: 2234 4503
PfoI T'CCnGG_A
Cuts at: 2063 2063
Size: 6737
PmeI GTTT'AAAC
Cuts at: 3736 3736
Size: 6737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1666 1698 5183 5215 1666
Size: 32 3485 32 3188
Fragments arranged by size:
3485 3188 32 32
PpuMI rG'GwC_Cy
Cuts at: 2018 3490 2018
Size: 1472 5265
PsiI TTA'TAA
Cuts at: 920 1277 1528 2212 2381 920
Size: 357 251 684 169 5276
Fragments arranged by size:
5276 684 357 251 169
PstI C_TGCA'G
Cuts at: 1608 3069 3784 4469 1608
Size: 1461 715 685 3876
Fragments arranged by size:
3876 1461 715 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6737
PvuII CAG'CTG
Cuts at: 2314 4162 4522 2314
Size: 1848 360 4529
Fragments arranged by size:
4529 1848 360
RsrII CG'GwC_CG
Cuts at: 4932 4932
Size: 6737
SacI G_AGCT'C
Cuts at: 3751 3751
Size: 6737
SalI G'TCGA_C
Cuts at: 3774 3774
Size: 6737
SapI GCTCTTCn'nnn_
Cuts at: 2156 3536 4760 4970 2156
Size: 1380 1224 210 3923
Fragments arranged by size:
3923 1380 1224 210
SbfI CC_TGCA'GG
Cuts at: 3784 3784
Size: 6737
ScaI AGT'ACT
Cuts at: 6254 6254
Size: 6737
SfcI C'TryA_G
Cuts at: 33 770 1604 1876 2265 2327 3065 3780 4465
Size: 737 834 272 389 62 738 715 685
Cuts at: 4465 5433 5624 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 834 738 737 715 685 389
272 191 62
SfoI GGC'GCC
Cuts at: 4169 4417 4169
Size: 248 6489
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6737
SmaI CCC'GGG
Cuts at: 3077 3759 3077
Size: 682 6055
SmlI C'TyrA_G
Cuts at: 2934 3892 4807 5521 5783 2934
Size: 958 915 714 262 3888
Fragments arranged by size:
3888 958 915 714 262
SnaBI TAC'GTA
Cuts at: 2652 2652
Size: 6737
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6737
SphI G_CATG'C
Cuts at: 3790 4290 3790
Size: 500 6237
SspI AAT'ATT
Cuts at: 498 1340 498
Size: 842 5895
StyI C'CwwG_G
Cuts at: 107 1725 1812 3634 3871 4225 107
Size: 1618 87 1822 237 354 2619
Fragments arranged by size:
2619 1822 1618 354 237 87
SwaI ATTT'AAAT
Cuts at: 1538 1538
Size: 6737
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1233 1432 1569 2988 4320 4453 4912 5121 1233
Size: 199 137 1419 1332 133 459 209 2849
Fragments arranged by size:
2849 1419 1332 459 209 199 137 133
TatI w'GTAC_w
Cuts at: 1060 1958 2894 6252 1060
Size: 898 936 3358 1545
Fragments arranged by size:
3358 1545 936 898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 3377 3409 6254 6286 3146
Size: 32 199 32 2845 32 3597
Fragments arranged by size:
3597 2845 199 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2137 4534 2137
Size: 2397 4340
XbaI T'CTAG_A
Cuts at: 2739 3768 2739
Size: 1029 5708
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 496 1437 3464 4232 496
Size: 941 2027 768 3001
Fragments arranged by size:
3001 2027 941 768
XmaI C'CCGG_G
Cuts at: 3075 3757 3075
Size: 682 6055
XmnI GAAnn'nnTTC
Cuts at: 404 988 2813 404
Size: 584 1825 4328
Fragments arranged by size:
4328 1825 584
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI ApaI
AscI BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BspEI BssHII BstXI BstZ17I ClaI DraIII EcoNI FseI
FspAI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI StuI XhoI ZraI