Restriction map for
pF1KA0674
(Circular) (Six-base) MAPSORT of: pF1KA0674.seq Check: 3475 from: 1 to: 6770
pF1KA0674 6770 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 594 2576 3054 594
Size: 1982 478 4310
Fragments arranged by size:
4310 1982 478
AccI GT'mk_AC
Cuts at: 3808 3808
Size: 6770
Acc65I G'GTAC_C
Cuts at: 3786 3786
Size: 6770
AclI AA'CG_TT
Cuts at: 6610 6610
Size: 6770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 1658 1951 2158 2614 2869 3389 4595 5027
Size: 331 293 207 456 255 520 1206 432
Cuts at: 5027 5774 6042 1327
Size: 747 268 2055
Fragments arranged by size:
2055 1206 747 520 456 432 331 293
268 255 207
AflIII A'CryG_T
Cuts at: 4122 5226 6203 4122
Size: 1104 977 4689
Fragments arranged by size:
4689 1104 977
AgeI A'CCGG_T
Cuts at: 3502 5158 3502
Size: 1656 5114
AleI CACnn'nnGTG
Cuts at: 2173 2173
Size: 6770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1768 1768
Size: 6770
AlwNI CAG_nnn'CTG
Cuts at: 202 2619 3148 4075 5642 202
Size: 2417 529 927 1567 1330
Fragments arranged by size:
2417 1567 1330 927 529
ApaI G_GGCC'C
Cuts at: 3227 3227
Size: 6770
ApaLI G'TGCA_C
Cuts at: 5540 5540
Size: 6770
ApoI r'AATT_y
Cuts at: 217 391 1661 2227 2396 3774 6123 6379 217
Size: 174 1270 566 169 1378 2349 256 608
Fragments arranged by size:
2349 1378 1270 608 566 256 174 169
AseI AT'TA_AT
Cuts at: 20 20
Size: 6770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6770
AvaI C'yCGr_G
Cuts at: 2453 3412 3790 2453
Size: 959 378 5433
Fragments arranged by size:
5433 959 378
AvrII C'CTAG_G
Cuts at: 3252 3252
Size: 6770
BamHI G'GATC_C
Cuts at: 3795 3795
Size: 6770
BanI G'GyrC_C
Cuts at: 160 1383 1413 3051 3786 4200 4448 4483 160
Size: 1223 30 1638 735 414 248 35 2447
Fragments arranged by size:
2447 1638 1223 735 414 248 35 30
BanII G_rGCy'C
Cuts at: 1409 2198 3227 3251 3784 4814 1409
Size: 789 1029 24 533 1030 3365
Fragments arranged by size:
3365 1030 1029 789 533 24
BbeI G_GCGC'C
Cuts at: 4204 4452 4204
Size: 248 6522
BbsI GAAGACnn'nnnn_
Cuts at: 2973 3686 2973
Size: 713 6057
BbvCI CC'TCA_GC
Cuts at: 1388 2991 3149 3461 1388
Size: 1603 158 312 4697
Fragments arranged by size:
4697 1603 312 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 751 785 920 954 1540 1574 751
Size: 34 135 34 586 34 5947
Fragments arranged by size:
5947 586 135 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3273 4668 5429 6770 3273
Size: 1395 761 1341 3273
Fragments arranged by size:
3273 1395 1341 761
BclI T'GATC_A
Cuts at: 4290 4290
Size: 6770
BfrBI ATG'CAT
Cuts at: 352 352
Size: 6770
BglI GCCn_nnn'nGGC
Cuts at: 169 1560 2653 169
Size: 1391 1093 4286
Fragments arranged by size:
4286 1391 1093
BglII A'GATC_T
Cuts at: 2148 4285 6322 2148
Size: 2137 2037 2596
Fragments arranged by size:
2596 2137 2037
BlpI GC'TnA_GC
Cuts at: 2504 3314 3882 2504
Size: 810 568 5392
Fragments arranged by size:
5392 810 568
Bme1580I G_kGCm'C
Cuts at: 3056 3227 4395 4488 5544 3056
Size: 171 1168 93 1056 4282
Fragments arranged by size:
4282 1168 1056 171 93
BmrI ACTGGGnnnn_n'
Cuts at: 1412 2511 3982 4063 4398 6542 1412
Size: 1099 1471 81 335 2144 1640
Fragments arranged by size:
2144 1640 1471 1099 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3174 3577 5137 3174
Size: 403 1560 4807
Fragments arranged by size:
4807 1560 403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1393 2302 2635 2839 869
Size: 524 909 333 204 4800
Fragments arranged by size:
4800 909 524 333 204
Bpu10I CC'TnA_GC
Cuts at: 1388 2991 3149 3245 3461 1388
Size: 1603 158 96 216 4697
Fragments arranged by size:
4697 1603 216 158 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 2251 2345 2996 3460 3946 4825 5317 5615
Size: 1340 94 651 464 486 879 492 298
Cuts at: 5615 911
Size: 2066
Fragments arranged by size:
2066 1340 879 651 492 486 464 298
94
BsaI GGTCTCn'nnnn_
Cuts at: 2067 6474 2067
Size: 4407 2363
BsaAI yAC'GTr
Cuts at: 4123 4123
Size: 6770
BsaBI GATnn'nnATC
Cuts at: 6 4284 6321 6
Size: 4278 2037 455
Fragments arranged by size:
4278 2037 455
BsaHI Gr'CG_yC
Cuts at: 3702 4201 4449 5151 6647 3702
Size: 499 248 702 1496 3825
Fragments arranged by size:
3825 1496 702 499 248
BsaWI w'CCGG_w
Cuts at: 2249 3502 3959 4480 5158 5432 5579 5990 2249
Size: 1253 457 521 678 274 147 411 3029
Fragments arranged by size:
3029 1253 678 521 457 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2050 2299 2395 2398 3083 3298 3430 3682 3685
Size: 249 96 3 685 215 132 252 3
Cuts at: 3685 2050
Size: 5135
Fragments arranged by size:
5135 685 252 249 215 132 96 3
3
BseYI C'CCAG_C
Cuts at: 747 827 843 1544 1572 3568 3581 3759 4195
Size: 80 16 701 28 1996 13 178 436
Cuts at: 4195 4953 5530 6026 747
Size: 758 577 496 1491
Fragments arranged by size:
1996 1491 758 701 577 496 436 178
80 28 16 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2197 2197
Size: 6770
BsiEI CG_ry'CG
Cuts at: 104 4312 4358 5566 104
Size: 4208 46 1208 1308
Fragments arranged by size:
4208 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 319 653 2198 2567 3784 4562 4752 5544 319
Size: 334 1545 369 1217 778 190 792 1545
Fragments arranged by size:
1545 1545 1217 792 778 369 334 190
BsmI GAATG_Cn'
Cuts at: 845 845
Size: 6770
BsmBI CGTCTCn'nnnn_
Cuts at: 1043 1043
Size: 6770
Bsp1286I G_dGCh'C
Cuts at: 319 653 1409 2198 2567 3056 3227 3251 3784
Size: 334 756 789 369 489 171 24 533
Cuts at: 3784 4395 4488 4562 4752 4814 5544 319
Size: 611 93 74 190 62 730 1545
Fragments arranged by size:
1545 789 756 730 611 533 489 369
334 190 171 93 74 62 24
BspEI T'CCGG_A
Cuts at: 5990 5990
Size: 6770
BspHI T'CATG_A
Cuts at: 1631 1706 2777 2858 5946 1631
Size: 75 1071 81 3088 2455
Fragments arranged by size:
3088 2455 1071 81 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 594 1489 2049 2576 3054 3526 3820 4034 4336
Size: 895 560 527 478 472 294 214 302
Cuts at: 4336 4717 594
Size: 381 2647
Fragments arranged by size:
2647 895 560 527 478 472 381 302
294 214
BsrBI CCG'CTC
Cuts at: 50 155 1581 2958 5062 5116 6600 6765 50
Size: 105 1426 1377 2104 54 1484 165 55
Fragments arranged by size:
2104 1484 1426 1377 165 105 55 54
BsrDI GCAATG_nn'
Cuts at: 611 4682 611
Size: 4071 2699
BsrFI r'CCGG_y
Cuts at: 721 1347 3502 4768 4949 5158 721
Size: 626 2155 1266 181 209 2333
Fragments arranged by size:
2333 2155 1266 626 209 181
BssSI C'ACGA_G
Cuts at: 5041 5399 5041
Size: 358 6412
BstAPI GCAn_nnn'nTGC
Cuts at: 349 1420 4147 349
Size: 1071 2727 2972
Fragments arranged by size:
2972 2727 1071
BstBI TT'CG_AA
Cuts at: 1665 5131 1665
Size: 3466 3304
BstXI CCAn_nnnn'nTGG
Cuts at: 330 2728 3306 330
Size: 2398 578 3794
Fragments arranged by size:
3794 2398 578
BstYI r'GATC_y
Cuts at: 1 1847 2148 3795 4262 4285 4620 4866 5878
Size: 1846 301 1647 467 23 335 246 1012
Cuts at: 5878 5964 5976 6322 1
Size: 86 12 346 449
Fragments arranged by size:
1846 1647 1012 467 449 346 335 301
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3095 3435 3665 3095
Size: 340 230 6200
Fragments arranged by size:
6200 340 230
BtgI C'CryG_G
Cuts at: 207 3047 3266 207
Size: 2840 219 3711
Fragments arranged by size:
3711 2840 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4720 4901 5064 4720
Size: 181 163 6426
Fragments arranged by size:
6426 181 163
BtsI GCAGTG_nn'
Cuts at: 653 767 2051 2934 4013 653
Size: 114 1284 883 1079 3410
Fragments arranged by size:
3410 1284 1079 883 114
ClaI AT'CG_AT
Cuts at: 357 357
Size: 6770
DraI TTT'AAA
Cuts at: 3624 3769 3624
Size: 145 6625
DrdI GACnn_nn'nnGTC
Cuts at: 2457 4476 5334 2457
Size: 2019 858 3893
Fragments arranged by size:
3893 2019 858
EaeI y'GGCC_r
Cuts at: 204 2048 2959 4355 4529 4920 4947 6292 204
Size: 1844 911 1396 174 391 27 1345 682
Fragments arranged by size:
1844 1396 1345 911 682 391 174 27
EagI C'GGCC_G
Cuts at: 4355 4355
Size: 6770
EarI CTCTTCn'nnn_
Cuts at: 639 1130 1346 1715 1945 2888 2912 2948 3602
Size: 491 216 369 230 943 24 36 654
Cuts at: 3602 4793 5003 639
Size: 1191 210 2406
Fragments arranged by size:
2406 1191 943 654 491 369 230 216
210 36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2541 4978 5288 5434 6580 2541
Size: 2437 310 146 1146 2731
Fragments arranged by size:
2731 2437 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2196 3782 2196
Size: 1586 5184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 1327 1393 1658 1951 2158 2302 2614 2635
Size: 458 66 265 293 207 144 312 21
Cuts at: 2635 2839 2869 3389 4595 5027 5774 6042 869
Size: 204 30 520 1206 432 747 268 1597
Fragments arranged by size:
1597 1206 747 520 458 432 312 293
268 265 207 204 144 66 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 251 2284 251
Size: 2033 4737
EcoO109I rG'GnC_Cy
Cuts at: 660 3099 3223 3224 3256 3423 3439 3909 660
Size: 2439 124 1 32 167 16 470 3521
Fragments arranged by size:
3521 2439 470 167 124 32 16 1
EcoRI G'AATT_C
Cuts at: 3774 3774
Size: 6770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 2337 4789 1867
Size: 470 2452 3848
Fragments arranged by size:
3848 2452 470
FspI TGC'GCA
Cuts at: 2057 2592 4551 2057
Size: 535 1959 4276
Fragments arranged by size:
4276 1959 535
FspAI rTGC'GCAy
Cuts at: 2592 2592
Size: 6770
HaeII r_GCGC'y
Cuts at: 4204 4452 5474 6453 4204
Size: 248 1022 979 4521
Fragments arranged by size:
4521 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 979 1011 1048 1080 1441 1473 2302
Size: 32 817 32 37 32 361 32 829
Cuts at: 2302 2334 2473 2505 2865 2897 3313 3345 4612
Size: 32 139 32 360 32 416 32 1267
Cuts at: 4612 4642 4644 4674 130
Size: 30 2 30 2226
Fragments arranged by size:
2226 1267 829 817 416 361 360 139
37 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 457 3809 457
Size: 3352 3418
KasI G'GCGC_C
Cuts at: 4200 4448 4200
Size: 248 6522
KpnI G_GTAC'C
Cuts at: 3790 3790
Size: 6770
MluI A'CGCG_T
Cuts at: 6203 6203
Size: 6770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 675 5441 5625 554
Size: 121 4766 184 1699
Fragments arranged by size:
4766 1699 184 121
MscI TGG'CCA
Cuts at: 206 4531 206
Size: 4325 2445
MslI CAynn'nnrTG
Cuts at: 212 1795 2173 4759 4886 212
Size: 1583 378 2586 127 2096
Fragments arranged by size:
2586 2096 1583 378 127
MspA1I CmG'CkG
Cuts at: 199 1254 1462 1548 2047 2076 2124 2476 2487
Size: 1055 208 86 499 29 48 352 11
Cuts at: 2487 2889 3881 4195 4555 5568 5813 199
Size: 402 992 314 360 1013 245 1156
Fragments arranged by size:
1156 1055 1013 992 499 402 360 352
314 245 208 86 48 29 11
NaeI GCC'GGC
Cuts at: 1349 4951 1349
Size: 3602 3168
NarI GG'CG_CC
Cuts at: 4201 4449 4201
Size: 248 6522
NcoI C'CATG_G
Cuts at: 207 3047 3266 207
Size: 2840 219 3711
Fragments arranged by size:
3711 2840 219
NgoMIV G'CCGG_C
Cuts at: 1347 4949 1347
Size: 3602 3168
NsiI A_TGCA'T
Cuts at: 354 354
Size: 6770
NspI r_CATG'y
Cuts at: 1770 2008 3823 4323 5230 1770
Size: 238 1815 500 907 3310
Fragments arranged by size:
3310 1815 907 500 238
PciI A'CATG_T
Cuts at: 5226 5226
Size: 6770
PmeI GTTT'AAAC
Cuts at: 3769 3769
Size: 6770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5909 5941 5909
Size: 32 6738
PpuMI rG'GwC_Cy
Cuts at: 660 3423 660
Size: 2763 4007
PspOMI G'GGCC_C
Cuts at: 3223 3223
Size: 6770
PspXI vC'TCGA_Gb
Cuts at: 2453 2453
Size: 6770
PstI C_TGCA'G
Cuts at: 405 1032 1553 2075 2117 2481 2573 3817 4502
Size: 627 521 522 42 364 92 1244 685
Cuts at: 4502 405
Size: 2673
Fragments arranged by size:
2673 1244 685 627 522 521 364 92
42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6770
PvuII CAG'CTG
Cuts at: 199 1254 1462 1548 2076 2124 2476 2487 2889
Size: 1055 208 86 528 48 352 11 402
Cuts at: 2889 4195 4555 199
Size: 1306 360 2414
Fragments arranged by size:
2414 1306 1055 528 402 360 352 208
86 48 11
RsrII CG'GwC_CG
Cuts at: 4965 4965
Size: 6770
SacI G_AGCT'C
Cuts at: 2198 3784 2198
Size: 1586 5184
SalI G'TCGA_C
Cuts at: 3807 3807
Size: 6770
SanDI GG'GwC_CC
Cuts at: 3423 3423
Size: 6770
SapI GCTCTTCn'nnn_
Cuts at: 1130 2948 4793 5003 1130
Size: 1818 1845 210 2897
Fragments arranged by size:
2897 1845 1818 210
SbfI CC_TGCA'GG
Cuts at: 3817 3817
Size: 6770
ScaI AGT'ACT
Cuts at: 6287 6287
Size: 6770
SexAI A'CCwGG_T
Cuts at: 2783 2783
Size: 6770
SfcI C'TryA_G
Cuts at: 33 401 522 686 1028 1549 2071 2113 2477
Size: 368 121 164 342 521 522 42 364
Cuts at: 2477 2509 2569 3813 4498 5491 5682 33
Size: 32 60 1244 685 993 191 1121
Fragments arranged by size:
1244 1121 993 685 522 521 368 364
342 191 164 121 60 42 32
SfoI GGC'GCC
Cuts at: 4202 4450 4202
Size: 248 6522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6770
SmaI CCC'GGG
Cuts at: 3792 3792
Size: 6770
SmlI C'TyrA_G
Cuts at: 926 2266 2360 2453 3011 3475 3925 4840 5332
Size: 1340 94 93 558 464 450 915 492
Cuts at: 5332 5594 926
Size: 262 2102
Fragments arranged by size:
2102 1340 915 558 492 464 450 262
94 93
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6770
SphI G_CATG'C
Cuts at: 2008 3823 4323 2008
Size: 1815 500 4455
Fragments arranged by size:
4455 1815 500
StyI C'CwwG_G
Cuts at: 207 1602 2024 2321 2555 3047 3252 3266 3904
Size: 1395 422 297 234 492 205 14 638
Cuts at: 3904 4258 207
Size: 354 2719
Fragments arranged by size:
2719 1395 638 492 422 354 297 234
205 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1104 4353 4486 4945 5154 1104
Size: 3249 133 459 209 2720
Fragments arranged by size:
3249 2720 459 209 133
TatI w'GTAC_w
Cuts at: 227 2496 6285 227
Size: 2269 3789 712
Fragments arranged by size:
3789 2269 712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3262 3294 6287 6319 3262
Size: 32 2993 32 3713
Fragments arranged by size:
3713 2993 32 32
Tth111I GACn'n_nGTC
Cuts at: 1323 4567 1323
Size: 3244 3526
XbaI T'CTAG_A
Cuts at: 3801 3801
Size: 6770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4265 4265
Size: 6770
XhoI C'TCGA_G
Cuts at: 2453 2453
Size: 6770
XmaI C'CCGG_G
Cuts at: 3790 3790
Size: 6770
XmnI GAAnn'nnTTC
Cuts at: 2970 2970
Size: 6770
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI BaeI BmgBI
BmtI BsaXI BsiWI BsrGI BssHII BstEII BstZ17I CspCI
DraIII EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PshAI
PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI