Restriction map for
pF1KA0672
(Circular) (Six-base) MAPSORT of: pF1KA0672.seq Check: 4819 from: 1 to: 5567
pF1KA0672 5567 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2419 2470 2605 2419
Size: 51 135 5381
Fragments arranged by size:
5381 135 51
Acc65I G'GTAC_C
Cuts at: 2583 2583
Size: 5567
AclI AA'CG_TT
Cuts at: 5407 5407
Size: 5567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 578 1024 1171 3392 3824 4571 4839 181
Size: 397 446 147 2221 432 747 268 909
Fragments arranged by size:
2221 909 747 446 432 397 268 147
AfeI AGC'GCT
Cuts at: 301 301
Size: 5567
AflIII A'CryG_T
Cuts at: 1491 2919 4023 5000 1491
Size: 1428 1104 977 2058
Fragments arranged by size:
2058 1428 1104 977
AgeI A'CCGG_T
Cuts at: 3955 3955
Size: 5567
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1403 1435 1441 1473 1403
Size: 32 6 32 5497
Fragments arranged by size:
5497 32 32 6
AlwNI CAG_nnn'CTG
Cuts at: 292 339 432 1800 1903 2518 2872 4439 292
Size: 47 93 1368 103 615 354 1567 1420
Fragments arranged by size:
1567 1420 1368 615 354 103 93 47
ApaI G_GGCC'C
Cuts at: 1859 1859
Size: 5567
ApaLI G'TGCA_C
Cuts at: 4337 4337
Size: 5567
ApoI r'AATT_y
Cuts at: 1255 2571 4920 5176 1255
Size: 1316 2349 256 1646
Fragments arranged by size:
2349 1646 1316 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5567
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
AvaI C'yCGr_G
Cuts at: 1561 1858 2211 2464 2587 1561
Size: 297 353 253 123 4541
Fragments arranged by size:
4541 353 297 253 123
BamHI G'GATC_C
Cuts at: 1148 2592 1148
Size: 1444 4123
BanI G'GyrC_C
Cuts at: 625 1553 1987 2061 2583 2997 3245 3280 625
Size: 928 434 74 522 414 248 35 2912
Fragments arranged by size:
2912 928 522 434 414 248 74 35
BanII G_rGCy'C
Cuts at: 476 1859 1968 1980 2465 2581 3611 476
Size: 1383 109 12 485 116 1030 2432
Fragments arranged by size:
2432 1383 1030 485 116 109 12
BbeI G_GCGC'C
Cuts at: 1557 1991 3001 3249 1557
Size: 434 1010 248 3875
Fragments arranged by size:
3875 1010 434 248
BbsI GAAGACnn'nnnn_
Cuts at: 194 584 194
Size: 390 5177
BbvCI CC'TCA_GC
Cuts at: 1582 2330 1582
Size: 748 4819
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 106 140 1747 1781 2541 2575 106
Size: 34 1607 34 760 34 3098
Fragments arranged by size:
3098 1607 760 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3465 4226 5567 3465
Size: 761 1341 3465
Fragments arranged by size:
3465 1341 761
BclI T'GATC_A
Cuts at: 3087 3087
Size: 5567
BglI GCCn_nnn'nGGC
Cuts at: 1762 1762
Size: 5567
BglII A'GATC_T
Cuts at: 2424 3082 5119 2424
Size: 658 2037 2872
Fragments arranged by size:
2872 2037 658
BlpI GC'TnA_GC
Cuts at: 1979 2679 1979
Size: 700 4867
Bme1580I G_kGCm'C
Cuts at: 1859 1939 3192 3285 4341 1859
Size: 80 1253 93 1056 3085
Fragments arranged by size:
3085 1253 1056 93 80
BmgBI CAC'GTC
Cuts at: 465 465
Size: 5567
BmrI ACTGGGnnnn_n'
Cuts at: 1298 1686 2128 2779 2860 3195 5339 1298
Size: 388 442 651 81 335 2144 1526
Fragments arranged by size:
2144 1526 651 442 388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2207 2333 3934 2207
Size: 126 1601 3840
Fragments arranged by size:
3840 1601 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 898 1630 1831 1880 1911 1994 2512 235
Size: 663 732 201 49 31 83 518 3290
Fragments arranged by size:
3290 732 663 518 201 83 49 31
Bpu10I CC'TnA_GC
Cuts at: 425 1582 2297 2330 425
Size: 1157 715 33 3662
Fragments arranged by size:
3662 1157 715 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 2743 3622 4114 4412 967
Size: 1776 879 492 298 2122
Fragments arranged by size:
2122 1776 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2304 5271 2304
Size: 2967 2600
BsaAI yAC'GTr
Cuts at: 1494 2920 1494
Size: 1426 4141
BsaBI GATnn'nnATC
Cuts at: 6 3081 5118 6
Size: 3075 2037 455
Fragments arranged by size:
3075 2037 455
BsaHI Gr'CG_yC
Cuts at: 1554 1988 2998 3246 3948 5444 1554
Size: 434 1010 248 702 1496 1677
Fragments arranged by size:
1677 1496 1010 702 434 248
BsaWI w'CCGG_w
Cuts at: 2039 2756 3277 3955 4229 4376 4787 2039
Size: 717 521 678 274 147 411 2819
Fragments arranged by size:
2819 717 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 286 316 1319 1349 1441 1471 2527 2557 286
Size: 30 1003 30 92 30 1056 30 3296
Fragments arranged by size:
3296 1056 1003 92 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 895 979 1686 2228 2318 2551 2554 895
Size: 84 707 542 90 233 3 3908
Fragments arranged by size:
3908 707 542 233 90 84 3
BseYI C'CCAG_C
Cuts at: 1996 2002 2158 2226 2992 3750 4327 4823 1996
Size: 6 156 68 766 758 577 496 2740
Fragments arranged by size:
2740 766 758 577 496 156 68 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 1889 1967 1066
Size: 823 78 4666
Fragments arranged by size:
4666 823 78
BsiEI CG_ry'CG
Cuts at: 104 903 1703 1707 3109 3155 4363 104
Size: 799 800 4 1402 46 1208 1308
Fragments arranged by size:
1402 1308 1208 800 799 46 4
BsiHKAI G_wGCw'C
Cuts at: 887 1980 2465 2498 2554 2581 3359 3549 4341
Size: 1093 485 33 56 27 778 190 792
Cuts at: 4341 887
Size: 2113
Fragments arranged by size:
2113 1093 792 778 485 190 56 33
27
BsmI GAATG_Cn'
Cuts at: 1206 1206
Size: 5567
BsmBI CGTCTCn'nnnn_
Cuts at: 400 455 400
Size: 55 5512
Bsp1286I G_dGCh'C
Cuts at: 476 887 1859 1939 1968 1980 2465 2498 2554
Size: 411 972 80 29 12 485 33 56
Cuts at: 2554 2581 3192 3285 3359 3549 3611 4341 476
Size: 27 611 93 74 190 62 730 1702
Fragments arranged by size:
1702 972 730 611 485 411 190 93
80 74 62 56 33 29 27 12
BspEI T'CCGG_A
Cuts at: 2039 4787 2039
Size: 2748 2819
BspHI T'CATG_A
Cuts at: 4743 4743
Size: 5567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2617 2831 3133 3514 2617
Size: 214 302 381 4670
Fragments arranged by size:
4670 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3859 3913 5397 5562 50
Size: 3809 54 1484 165 55
Fragments arranged by size:
3809 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3479 3479
Size: 5567
BsrFI r'CCGG_y
Cuts at: 1550 1574 1702 3565 3746 3955 1550
Size: 24 128 1863 181 209 3162
Fragments arranged by size:
3162 1863 209 181 128 24
BsrGI T'GTAC_A
Cuts at: 710 710
Size: 5567
BssSI C'ACGA_G
Cuts at: 3838 4196 3838
Size: 358 5209
BstAPI GCAn_nnn'nTGC
Cuts at: 2944 2944
Size: 5567
BstBI TT'CG_AA
Cuts at: 3928 3928
Size: 5567
BstXI CCAn_nnnn'nTGG
Cuts at: 675 1311 2119 675
Size: 636 808 4123
Fragments arranged by size:
4123 808 636
BstYI r'GATC_y
Cuts at: 1 1148 2424 2592 3059 3082 3417 3663 4675
Size: 1147 1276 168 467 23 335 246 1012
Cuts at: 4675 4761 4773 5119 1
Size: 86 12 346 449
Fragments arranged by size:
1276 1147 1012 467 449 346 335 246
168 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 643 1633 2192 643
Size: 990 559 4018
Fragments arranged by size:
4018 990 559
BtgI C'CryG_G
Cuts at: 1592 1811 2372 1592
Size: 219 561 4787
Fragments arranged by size:
4787 561 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3517 3698 3861 3517
Size: 181 163 5223
Fragments arranged by size:
5223 181 163
BtsI GCAGTG_nn'
Cuts at: 2810 2810
Size: 5567
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1661 1696 1661
Size: 35 5532
DraI TTT'AAA
Cuts at: 2566 2566
Size: 5567
DrdI GACnn_nn'nnGTC
Cuts at: 588 1383 3273 4131 588
Size: 795 1890 858 2024
Fragments arranged by size:
2024 1890 858 795
EaeI y'GGCC_r
Cuts at: 140 722 900 1342 1700 2088 2121 3152 3326
Size: 582 178 442 358 388 33 1031 174
Cuts at: 3326 3717 3744 5089 140
Size: 391 27 1345 618
Fragments arranged by size:
1345 1031 618 582 442 391 388 358
178 174 33 27
EagI C'GGCC_G
Cuts at: 900 1700 3152 900
Size: 800 1452 3315
Fragments arranged by size:
3315 1452 800
EarI CTCTTCn'nnn_
Cuts at: 653 979 1683 3590 3800 653
Size: 326 704 1907 210 2420
Fragments arranged by size:
2420 1907 704 326 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 505 2467 3775 4085 4231 5377 505
Size: 1962 1308 310 146 1146 695
Fragments arranged by size:
1962 1308 1146 695 310 146
EcoICRI GAG'CTC
Cuts at: 1978 2463 2579 1978
Size: 485 116 4966
Fragments arranged by size:
4966 485 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 235 578 898 1024 1171 1630 1831 1880
Size: 54 343 320 126 147 459 201 49
Cuts at: 1880 1911 1994 2512 3392 3824 4571 4839 181
Size: 31 83 518 880 432 747 268 909
Fragments arranged by size:
909 880 747 518 459 432 343 320
268 201 147 126 83 54 49 31
EcoNI CCTnn'n_nnAGG
Cuts at: 196 196
Size: 5567
EcoO109I rG'GnC_Cy
Cuts at: 1327 2079 2379 2706 1327
Size: 752 300 327 4188
Fragments arranged by size:
4188 752 327 300
EcoRI G'AATT_C
Cuts at: 2571 2571
Size: 5567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 211 879 2100 3586 211
Size: 668 1221 1486 2192
Fragments arranged by size:
2192 1486 1221 668
FspI TGC'GCA
Cuts at: 3348 3348
Size: 5567
HaeII r_GCGC'y
Cuts at: 303 1557 1991 3001 3249 4271 5250 303
Size: 1254 434 1010 248 1022 979 620
Fragments arranged by size:
1254 1022 1010 979 620 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 682 714 1319 1351 2383 2415 2431
Size: 32 364 32 605 32 1032 32 16
Cuts at: 2431 2452 2463 2484 2527 2559 3409 3439 3441
Size: 21 11 21 43 32 850 30 2
Cuts at: 3441 3471 286
Size: 30 2382
Fragments arranged by size:
2382 1032 850 605 364 43 32 32
32 32 32 30 30 21 21 16
11 2
HincII GTy'rAC
Cuts at: 2471 2606 2471
Size: 135 5432
HindIII A'AGCT_T
Cuts at: 1183 1183
Size: 5567
KasI G'GCGC_C
Cuts at: 1553 1987 2997 3245 1553
Size: 434 1010 248 3875
Fragments arranged by size:
3875 1010 434 248
KpnI G_GTAC'C
Cuts at: 2587 2587
Size: 5567
MfeI C'AATT_G
Cuts at: 1073 1316 1073
Size: 243 5324
MluI A'CGCG_T
Cuts at: 5000 5000
Size: 5567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1687 4238 4422 1687
Size: 2551 184 2832
Fragments arranged by size:
2832 2551 184
MscI TGG'CCA
Cuts at: 142 724 1344 2090 2123 3328 142
Size: 582 620 746 33 1205 2381
Fragments arranged by size:
2381 1205 746 620 582 33
MslI CAynn'nnrTG
Cuts at: 1654 3556 3683 1654
Size: 1902 127 3538
Fragments arranged by size:
3538 1902 127
MspA1I CmG'CkG
Cuts at: 138 703 876 899 1785 1813 2119 2127 2230
Size: 565 173 23 886 28 306 8 103
Cuts at: 2230 2678 2992 3352 4365 4610 138
Size: 448 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 886 565 448 360 314 306
245 173 103 28 23 8
NaeI GCC'GGC
Cuts at: 1576 3748 1576
Size: 2172 3395
NarI GG'CG_CC
Cuts at: 1554 1988 2998 3246 1554
Size: 434 1010 248 3875
Fragments arranged by size:
3875 1010 434 248
NgoMIV G'CCGG_C
Cuts at: 1574 3746 1574
Size: 2172 3395
NspI r_CATG'y
Cuts at: 132 266 1429 2400 2418 2502 2620 3120 4027
Size: 134 1163 971 18 84 118 500 907
Cuts at: 4027 132
Size: 1672
Fragments arranged by size:
1672 1163 971 907 500 134 118 84
18
PasI CC'CwG_GG
Cuts at: 1444 2404 1444
Size: 960 4607
PciI A'CATG_T
Cuts at: 4023 4023
Size: 5567
PflMI CCAn_nnn'nTGG
Cuts at: 153 1650 153
Size: 1497 4070
PfoI T'CCnGG_A
Cuts at: 1166 1166
Size: 5567
PmeI GTTT'AAAC
Cuts at: 2566 2566
Size: 5567
PmlI CAC'GTG
Cuts at: 1494 1494
Size: 5567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1441 1473 4706 4738 1441
Size: 32 3233 32 2270
Fragments arranged by size:
3233 2270 32 32
PpuMI rG'GwC_Cy
Cuts at: 1327 2079 1327
Size: 752 4815
PshAI GACnn'nnGTC
Cuts at: 2024 2024
Size: 5567
PspOMI G'GGCC_C
Cuts at: 1855 1855
Size: 5567
PstI C_TGCA'G
Cuts at: 272 2019 2235 2614 3299 272
Size: 1747 216 379 685 2540
Fragments arranged by size:
2540 1747 685 379 216
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5567
PvuII CAG'CTG
Cuts at: 138 703 2127 2230 2992 3352 138
Size: 565 1424 103 762 360 2353
Fragments arranged by size:
2353 1424 762 565 360 103
RsrII CG'GwC_CG
Cuts at: 3762 3762
Size: 5567
SacI G_AGCT'C
Cuts at: 1980 2465 2581 1980
Size: 485 116 4966
Fragments arranged by size:
4966 485 116
SacII CC_GC'GG
Cuts at: 1814 1814
Size: 5567
SalI G'TCGA_C
Cuts at: 2469 2604 2469
Size: 135 5432
SapI GCTCTTCn'nnn_
Cuts at: 3590 3800 3590
Size: 210 5357
SbfI CC_TGCA'GG
Cuts at: 2614 2614
Size: 5567
ScaI AGT'ACT
Cuts at: 1054 5084 1054
Size: 4030 1537
SexAI A'CCwGG_T
Cuts at: 568 568
Size: 5567
SfcI C'TryA_G
Cuts at: 33 268 1512 2015 2231 2420 2610 3295 4288
Size: 235 1244 503 216 189 190 685 993
Cuts at: 4288 4479 33
Size: 191 1121
Fragments arranged by size:
1244 1121 993 685 503 235 216 191
190 189
SfoI GGC'GCC
Cuts at: 1555 1989 2999 3247 1555
Size: 434 1010 248 3875
Fragments arranged by size:
3875 1010 434 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
SmaI CCC'GGG
Cuts at: 2589 2589
Size: 5567
SmlI C'TyrA_G
Cuts at: 946 2722 3637 4129 4391 946
Size: 1776 915 492 262 2122
Fragments arranged by size:
2122 1776 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5567
SphI G_CATG'C
Cuts at: 132 1429 2620 3120 132
Size: 1297 1191 500 2579
Fragments arranged by size:
2579 1297 1191 500
SspI AAT'ATT
Cuts at: 510 510
Size: 5567
StuI AGG'CCT
Cuts at: 844 844
Size: 5567
StyI C'CwwG_G
Cuts at: 629 1220 2075 2204 2701 3055 629
Size: 591 855 129 497 354 3141
Fragments arranged by size:
3141 855 591 497 354 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1642 1668 2088 3150 3283 3742 3951 1642
Size: 26 420 1062 133 459 209 3258
Fragments arranged by size:
3258 1062 459 420 209 133 26
TatI w'GTAC_w
Cuts at: 710 1052 1488 5082 710
Size: 342 436 3594 1195
Fragments arranged by size:
3594 1195 436 342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 775 807 1191 1223 2063 2095 5084 5116 775
Size: 32 384 32 840 32 2989 32 1226
Fragments arranged by size:
2989 1226 840 384 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3364 3364
Size: 5567
XbaI T'CTAG_A
Cuts at: 2598 2598
Size: 5567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 543 3062 150
Size: 393 2519 2655
Fragments arranged by size:
2655 2519 393
XmaI C'CCGG_G
Cuts at: 2587 2587
Size: 5567
XmnI GAAnn'nnTTC
Cuts at: 118 191 118
Size: 73 5494
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI AvrII
BaeI BfrBI BmtI BsiWI BssHII BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI