Restriction map for
pF1KA0641
(Circular) (Six-base) MAPSORT of: pF1KA0641.seq Check: 1059 from: 1 to: 6926
pF1KA0641 6926 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 822 983 1470 2073 2475 3019 822
Size: 161 487 603 402 544 4729
Fragments arranged by size:
4729 603 544 487 402 161
AatII G_ACGT'C
Cuts at: 1556 3512 1556
Size: 1956 4970
AccI GT'mk_AC
Cuts at: 1550 3518 3964 1550
Size: 1968 446 4512
Fragments arranged by size:
4512 1968 446
Acc65I G'GTAC_C
Cuts at: 1964 3942 1964
Size: 1978 4948
AclI AA'CG_TT
Cuts at: 6766 6766
Size: 6926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 358 2541 3908 4751 5183 5536 6198 202
Size: 156 2183 1367 843 432 353 662 930
Fragments arranged by size:
2183 1367 930 843 662 432 353 156
AfeI AGC'GCT
Cuts at: 2600 2600
Size: 6926
AflIII A'CryG_T
Cuts at: 1841 2417 3851 4278 6078 6359 1841
Size: 576 1434 427 1800 281 2408
Fragments arranged by size:
2408 1800 1434 576 427 281
AhdI GACnn_n'nnGTC
Cuts at: 2523 3870 2523
Size: 1347 5579
AleI CACnn'nnGTG
Cuts at: 154 3850 154
Size: 3696 3230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 296 296
Size: 6926
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2617 2649 2617
Size: 32 6894
AlwNI CAG_nnn'CTG
Cuts at: 309 363 837 1311 1871 2002 2058 3011 4231
Size: 54 474 474 560 131 56 953 1220
Cuts at: 4231 5669 309
Size: 1438 1566
Fragments arranged by size:
1566 1438 1220 953 560 474 474 131
56 54
ApaI G_GGCC'C
Cuts at: 336 1076 1082 1097 1646 2716 2723 3077 3158
Size: 740 6 15 549 1070 7 354 81
Cuts at: 3158 3205 336
Size: 47 4057
Fragments arranged by size:
4057 1070 740 549 354 81 47 15
7 6
ApaLI G'TGCA_C
Cuts at: 547 970 5764 547
Size: 423 4794 1709
Fragments arranged by size:
4794 1709 423
ApoI r'AATT_y
Cuts at: 3930 6279 6535 3930
Size: 2349 256 4321
Fragments arranged by size:
4321 2349 256
AscI GG'CGCG_CC
Cuts at: 1328 1328
Size: 6926
AseI AT'TA_AT
Cuts at: 20 20
Size: 6926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6926
AvaI C'yCGr_G
Cuts at: 943 1075 1335 1954 2350 2716 2722 2800 2860
Size: 132 260 619 396 366 6 78 60
Cuts at: 2860 2953 3018 3545 3553 3567 3946 943
Size: 93 65 527 8 14 379 3923
Fragments arranged by size:
3923 619 527 396 379 366 260 132
93 78 65 60 14 8 6
BamHI G'GATC_C
Cuts at: 3951 3951
Size: 6926
BanI G'GyrC_C
Cuts at: 672 1003 1755 1964 2061 2361 2457 3208 3369
Size: 331 752 209 97 300 96 751 161
Cuts at: 3369 3378 3571 3895 3942 4356 4604 4639 672
Size: 9 193 324 47 414 248 35 2959
Fragments arranged by size:
2959 752 751 414 331 324 300 248
209 193 161 97 96 47 35 9
BanII G_rGCy'C
Cuts at: 336 788 1076 1082 1097 1121 1285 1646 1750
Size: 452 288 6 15 24 164 361 104
Cuts at: 1750 1991 2138 2220 2678 2716 2723 2859 3005
Size: 241 147 82 458 38 7 136 146
Cuts at: 3005 3047 3077 3158 3205 3222 3323 3554 3563
Size: 42 30 81 47 17 101 231 9
Cuts at: 3563 3610 3940 4970 336
Size: 47 330 1030 2292
Fragments arranged by size:
2292 1030 458 452 361 330 288 241
231 164 147 146 136 104 101 82
81 47 47 42 38 30 24 17
15 9 7 6
BbeI G_GCGC'C
Cuts at: 1007 1759 3373 3575 4360 4608 1007
Size: 752 1614 202 785 248 3325
Fragments arranged by size:
3325 1614 785 752 248 202
BbsI GAAGACnn'nnnn_
Cuts at: 2547 2547
Size: 6926
BbvCI CC'TCA_GC
Cuts at: 1913 2156 2390 2529 2807 1913
Size: 243 234 139 278 6032
Fragments arranged by size:
6032 278 243 234 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 514 525 548 559 708 742 2287 2321 514
Size: 11 23 11 149 34 1545 34 5119
Fragments arranged by size:
5119 1545 149 34 34 23 11 11
BciVI GTATCCnnnnn_n'
Cuts at: 4824 5880 6926 4824
Size: 1056 1046 4824
Fragments arranged by size:
4824 1056 1046
BclI T'GATC_A
Cuts at: 4446 4446
Size: 6926
BglI GCCn_nnn'nGGC
Cuts at: 1270 1513 2152 3020 1270
Size: 243 639 868 5176
Fragments arranged by size:
5176 868 639 243
BglII A'GATC_T
Cuts at: 4441 6478 4441
Size: 2037 4889
BlpI GC'TnA_GC
Cuts at: 1279 1354 4038 1279
Size: 75 2684 4167
Fragments arranged by size:
4167 2684 75
Bme1580I G_kGCm'C
Cuts at: 252 336 551 802 974 1076 1082 1097 1646
Size: 84 215 251 172 102 6 15 549
Cuts at: 1646 1732 1829 2164 2247 2716 2723 3077 3158
Size: 86 97 335 83 469 7 354 81
Cuts at: 3158 3205 3383 4551 4644 5768 252
Size: 47 178 1168 93 1124 1410
Fragments arranged by size:
1410 1168 1124 549 469 354 335 251
215 178 172 102 97 93 86 84
83 81 47 15 7 6
BmgBI CAC'GTC
Cuts at: 156 1191 1733 2420 3830 156
Size: 1035 542 687 1410 3252
Fragments arranged by size:
3252 1410 1035 687 542
BmrI ACTGGGnnnn_n'
Cuts at: 363 427 1448 1692 1735 1919 2897 3087 4138
Size: 64 1021 244 43 184 978 190 1051
Cuts at: 4138 4219 4554 6698 363
Size: 81 335 2144 591
Fragments arranged by size:
2144 1051 1021 978 591 335 244 190
184 81 64 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 249 2415 2715 2838 3241 5293 249
Size: 2166 300 123 403 2052 1882
Fragments arranged by size:
2166 2052 1882 403 300 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 340 1168 1975 1991 2603 331
Size: 9 828 807 16 612 4654
Fragments arranged by size:
4654 828 807 612 16 9
Bpu10I CC'TnA_GC
Cuts at: 1749 1913 2156 2390 2529 2807 1749
Size: 164 243 234 139 278 5868
Fragments arranged by size:
5868 278 243 234 164 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 412 850 2635 3415 4102 4981 5695 5993
Size: 285 438 1785 780 687 879 714 298
Cuts at: 5993 127
Size: 1060
Fragments arranged by size:
1785 1060 879 780 714 687 438 298
285
BsaI GGTCTCn'nnnn_
Cuts at: 1201 3448 6630 1201
Size: 2247 3182 1497
Fragments arranged by size:
3182 2247 1497
BsaAI yAC'GTr
Cuts at: 3852 4279 3852
Size: 427 6499
BsaBI GATnn'nnATC
Cuts at: 6 4440 6477 6
Size: 4434 2037 455
Fragments arranged by size:
4434 2037 455
BsaHI Gr'CG_yC
Cuts at: 385 518 1004 1553 1756 2252 3370 3509 3572
Size: 133 486 549 203 496 1118 139 63
Cuts at: 3572 3866 4357 4605 5307 6803 385
Size: 294 491 248 702 1496 508
Fragments arranged by size:
1496 1118 702 549 508 496 491 486
294 248 203 139 133 63
BsaWI w'CCGG_w
Cuts at: 1347 2167 3039 4115 4636 5314 5725 5872 6146
Size: 820 872 1076 521 678 411 147 274
Cuts at: 6146 1347
Size: 2127
Fragments arranged by size:
2127 1076 872 820 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1819 1849 3777 3807 1819
Size: 30 1928 30 4938
Fragments arranged by size:
4938 1928 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 328 979 1033 1036 1417 1679 1807 2248
Size: 198 651 54 3 381 262 128 441
Cuts at: 2248 3289 3292 3295 3298 3331 3379 130
Size: 1041 3 3 3 33 48 3677
Fragments arranged by size:
3677 1041 651 441 381 262 198 128
54 48 33 3 3 3 3
BseYI C'CCAG_C
Cuts at: 795 815 881 1101 1374 1530 1817 2365 2871
Size: 20 66 220 273 156 287 548 506
Cuts at: 2871 3004 3054 3076 3082 3439 3619 4351 5109
Size: 133 50 22 6 357 180 732 758
Cuts at: 5109 5774 6182 795
Size: 665 408 1539
Fragments arranged by size:
1539 758 732 665 548 506 408 357
287 273 220 180 156 133 66 50
22 20 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 310 340 508 154
Size: 156 30 168 6572
Fragments arranged by size:
6572 168 156 30
BsiEI CG_ry'CG
Cuts at: 104 195 3271 3637 3758 4468 4514 5744 104
Size: 91 3076 366 121 710 46 1230 1286
Fragments arranged by size:
3076 1286 1230 710 366 121 91 46
BsiHKAI G_wGCw'C
Cuts at: 551 788 974 1121 1355 1750 2651 2887 3323
Size: 237 186 147 234 395 901 236 436
Cuts at: 3323 3554 3940 4718 4908 5768 551
Size: 231 386 778 190 860 1709
Fragments arranged by size:
1709 901 860 778 436 395 386 237
236 234 231 190 186 147
BsmBI CGTCTCn'nnnn_
Cuts at: 1899 1899
Size: 6926
Bsp1286I G_dGCh'C
Cuts at: 252 336 551 788 802 974 1076 1082 1097
Size: 84 215 237 14 172 102 6 15
Cuts at: 1097 1121 1285 1355 1646 1732 1750 1829 1991
Size: 24 164 70 291 86 18 79 162
Cuts at: 1991 2138 2164 2220 2247 2651 2678 2716 2723
Size: 147 26 56 27 404 27 38 7
Cuts at: 2723 2859 2887 3005 3047 3077 3158 3205 3222
Size: 136 28 118 42 30 81 47 17
Cuts at: 3222 3323 3383 3554 3563 3610 3940 4551 4644
Size: 101 60 171 9 47 330 611 93
Cuts at: 4644 4718 4908 4970 5768 252
Size: 74 190 62 798 1410
Fragments arranged by size:
1410 798 611 404 330 291 237 215
190 172 171 164 162 147 136 118
102 101 93 86 84 81 79 74
70 62 60 56 47 47 42 38
30 28 27 27 26 24 18 17
15 14 9 7 6
BspEI T'CCGG_A
Cuts at: 3039 3039
Size: 6926
BspHI T'CATG_A
Cuts at: 5358 5358
Size: 6926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 359 401 446 731 822 983 1470 2073 2475
Size: 42 45 285 91 161 487 603 402
Cuts at: 2475 2895 3019 3425 3976 4190 4492 4873 359
Size: 420 124 406 551 214 302 381 2412
Fragments arranged by size:
2412 603 551 487 420 406 402 381
302 285 214 161 124 91 45 42
BsrBI CCG'CTC
Cuts at: 50 749 1608 1669 2471 3793 5218 5272 6756
Size: 699 859 61 802 1322 1425 54 1484
Cuts at: 6756 6921 50
Size: 165 55
Fragments arranged by size:
1484 1425 1322 859 802 699 165 61
55 54
BsrDI GCAATG_nn'
Cuts at: 4838 4838
Size: 6926
BsrFI r'CCGG_y
Cuts at: 161 1420 1441 3177 3266 3372 3632 4924 5105
Size: 1259 21 1736 89 106 260 1292 181
Cuts at: 5105 161
Size: 1982
Fragments arranged by size:
1982 1736 1292 1259 260 181 106 89
21
BssHII G'CGCG_C
Cuts at: 1328 1358 3407 3409 1328
Size: 30 2049 2 4845
Fragments arranged by size:
4845 2049 30 2
BssSI C'ACGA_G
Cuts at: 5197 5905 5197
Size: 708 6218
BstAPI GCAn_nnn'nTGC
Cuts at: 978 2002 4303 978
Size: 1024 2301 3601
Fragments arranged by size:
3601 2301 1024
BstBI TT'CG_AA
Cuts at: 5287 5287
Size: 6926
BstEII G'GTnAC_C
Cuts at: 1200 2116 1200
Size: 916 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 156 1883 2316 156
Size: 1727 433 4766
Fragments arranged by size:
4766 1727 433
BstYI r'GATC_y
Cuts at: 1 3951 4418 4441 4776 5022 5328 5340 5426
Size: 3950 467 23 335 246 306 12 86
Cuts at: 5426 6478 1
Size: 1052 449
Fragments arranged by size:
3950 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 2400 2823 3598 2400
Size: 423 775 5728
Fragments arranged by size:
5728 775 423
BtgI C'CryG_G
Cuts at: 107 195 464 1251 1469 1517 1536 1566 1942
Size: 88 269 787 218 48 19 30 376
Cuts at: 1942 2147 3383 3740 107
Size: 205 1236 357 3293
Fragments arranged by size:
3293 1236 787 376 357 269 218 205
88 48 30 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 517 4876 5057 5220 517
Size: 4359 181 163 2223
Fragments arranged by size:
4359 2223 181 163
BtsI GCAGTG_nn'
Cuts at: 252 624 2376 2971 4169 252
Size: 372 1752 595 1198 3009
Fragments arranged by size:
3009 1752 1198 595 372
DraI TTT'AAA
Cuts at: 3925 3925
Size: 6926
DrdI GACnn_nn'nnGTC
Cuts at: 3514 4632 5976 3514
Size: 1118 1344 4464
Fragments arranged by size:
4464 1344 1118
EaeI y'GGCC_r
Cuts at: 110 192 1271 1287 1422 1514 1691 2567 3268
Size: 82 1079 16 135 92 177 876 701
Cuts at: 3268 3634 3722 3755 4511 4685 5076 5103 6448
Size: 366 88 33 756 174 391 27 1345
Cuts at: 6448 110
Size: 588
Fragments arranged by size:
1345 1079 876 756 701 588 391 366
177 174 135 92 88 82 33 27
16
EagI C'GGCC_G
Cuts at: 192 3268 3634 3755 4511 192
Size: 3076 366 121 756 2607
Fragments arranged by size:
3076 2607 756 366 121
EarI CTCTTCn'nnn_
Cuts at: 3356 3671 4949 5159 3356
Size: 315 1278 210 5123
Fragments arranged by size:
5123 1278 315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 472 1384 1672 3217 5134 5876 6022 6736 472
Size: 912 288 1545 1917 742 146 714 662
Fragments arranged by size:
1917 1545 912 742 714 662 288 146
EcoICRI GAG'CTC
Cuts at: 786 1119 1748 3321 3552 3938 786
Size: 333 629 1573 231 386 3774
Fragments arranged by size:
3774 1573 629 386 333 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 331 340 358 1168 1975 1991 2541 2603
Size: 129 9 18 810 807 16 550 62
Cuts at: 2603 3908 4751 5183 5536 6198 202
Size: 1305 843 432 353 662 930
Fragments arranged by size:
1305 930 843 810 807 662 550 432
353 129 62 18 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 223 313 1402 223
Size: 90 1089 5747
Fragments arranged by size:
5747 1089 90
EcoO109I rG'GnC_Cy
Cuts at: 332 333 423 1072 1093 1602 1642 1643 1719
Size: 1 90 649 21 509 40 1 76
Cuts at: 1719 1887 2055 2492 2712 2713 2990 3154 3155
Size: 168 168 437 220 1 277 164 1
Cuts at: 3155 3201 3468 3883 4065 332
Size: 46 267 415 182 3193
Fragments arranged by size:
3193 649 509 437 415 277 267 220
182 168 168 164 90 76 46 40
21 1 1 1 1
EcoRI G'AATT_C
Cuts at: 3930 3930
Size: 6926
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3750 4945 3750
Size: 1195 5731
FseI GG_CCGG'CC
Cuts at: 3181 3270 3181
Size: 89 6837
FspI TGC'GCA
Cuts at: 3421 4707 3421
Size: 1286 5640
HaeII r_GCGC'y
Cuts at: 129 1007 1038 1044 1759 1836 2602 3373 3575
Size: 878 31 6 715 77 766 771 202
Cuts at: 3575 3626 4360 4608 5838 6609 129
Size: 51 734 248 1230 771 446
Fragments arranged by size:
1230 878 771 771 766 734 715 446
248 202 77 51 31 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1503 1879 1911 2509 2541 3856 3888 4768
Size: 32 376 32 598 32 1315 32 880
Cuts at: 4768 4798 4800 4830 1471
Size: 30 2 30 3567
Fragments arranged by size:
3567 1315 880 598 376 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1551 3965 1551
Size: 2414 4512
HindIII A'AGCT_T
Cuts at: 1590 2140 1590
Size: 550 6376
KasI G'GCGC_C
Cuts at: 1003 1755 3369 3571 4356 4604 1003
Size: 752 1614 202 785 248 3325
Fragments arranged by size:
3325 1614 785 752 248 202
KpnI G_GTAC'C
Cuts at: 1968 3946 1968
Size: 1978 4948
MluI A'CGCG_T
Cuts at: 6359 6359
Size: 6926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 881 1862 5685 5869 881
Size: 981 3823 184 1938
Fragments arranged by size:
3823 1938 981 184
MscI TGG'CCA
Cuts at: 112 1516 4687 112
Size: 1404 3171 2351
Fragments arranged by size:
3171 2351 1404
MslI CAynn'nnrTG
Cuts at: 154 3850 4915 5042 154
Size: 3696 1065 127 2038
Fragments arranged by size:
3696 2038 1065 127
MspA1I CmG'CkG
Cuts at: 666 885 891 908 951 1126 1143 1253 1471
Size: 219 6 17 43 175 17 110 218
Cuts at: 1471 1527 1568 1764 1770 1862 1871 3086 3120
Size: 56 41 196 6 92 9 1215 34
Cuts at: 3120 3326 3673 3714 4037 4351 4711 5495 5740
Size: 206 347 41 323 314 360 784 245
Cuts at: 5740 666
Size: 1852
Fragments arranged by size:
1852 1215 784 360 347 323 314 245
219 218 206 196 175 110 92 56
43 41 41 34 17 17 9 6
6
NaeI GCC'GGC
Cuts at: 163 1422 1443 3179 3268 3374 5107 163
Size: 1259 21 1736 89 106 1733 1982
Fragments arranged by size:
1982 1736 1733 1259 106 89 21
NarI GG'CG_CC
Cuts at: 1004 1756 3370 3572 4357 4605 1004
Size: 752 1614 202 785 248 3325
Fragments arranged by size:
3325 1614 785 752 248 202
NcoI C'CATG_G
Cuts at: 107 464 1517 3740 107
Size: 357 1053 2223 3293
Fragments arranged by size:
3293 2223 1053 357
NgoMIV G'CCGG_C
Cuts at: 161 1420 1441 3177 3266 3372 5105 161
Size: 1259 21 1736 89 106 1733 1982
Fragments arranged by size:
1982 1736 1733 1259 106 89 21
NspI r_CATG'y
Cuts at: 3979 4479 6082 3979
Size: 500 1603 4823
Fragments arranged by size:
4823 1603 500
PasI CC'CwG_GG
Cuts at: 765 1559 2052 765
Size: 794 493 5639
Fragments arranged by size:
5639 794 493
PciI A'CATG_T
Cuts at: 6078 6078
Size: 6926
PflMI CCAn_nnn'nTGG
Cuts at: 258 363 2058 258
Size: 105 1695 5126
Fragments arranged by size:
5126 1695 105
PfoI T'CCnGG_A
Cuts at: 308 851 2089 3338 308
Size: 543 1238 1249 3896
Fragments arranged by size:
3896 1249 1238 543
PmeI GTTT'AAAC
Cuts at: 3925 3925
Size: 6926
PmlI CAC'GTG
Cuts at: 3852 3852
Size: 6926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5372 5404 5372
Size: 32 6894
PpuMI rG'GwC_Cy
Cuts at: 423 1602 1719 1887 2492 2990 3468 3883 423
Size: 1179 117 168 605 498 478 415 3466
Fragments arranged by size:
3466 1179 605 498 478 415 168 117
PshAI GACnn'nnGTC
Cuts at: 3168 3168
Size: 6926
PspOMI G'GGCC_C
Cuts at: 332 1072 1078 1093 1642 2712 2719 3073 3154
Size: 740 6 15 549 1070 7 354 81
Cuts at: 3154 3201 332
Size: 47 4057
Fragments arranged by size:
4057 1070 740 549 354 81 47 15
7 6
PspXI vC'TCGA_Gb
Cuts at: 2800 2800
Size: 6926
PstI C_TGCA'G
Cuts at: 275 491 890 941 1307 2438 2480 2981 3973
Size: 216 399 51 366 1131 42 501 992
Cuts at: 3973 4658 275
Size: 685 2543
Fragments arranged by size:
2543 1131 992 685 501 399 366 216
51 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6926
PvuII CAG'CTG
Cuts at: 666 885 891 3086 4351 4711 666
Size: 219 6 2195 1265 360 2881
Fragments arranged by size:
2881 2195 1265 360 219 6
RsrII CG'GwC_CG
Cuts at: 849 903 5121 849
Size: 54 4218 2654
Fragments arranged by size:
4218 2654 54
SacI G_AGCT'C
Cuts at: 788 1121 1750 3323 3554 3940 788
Size: 333 629 1573 231 386 3774
Fragments arranged by size:
3774 1573 629 386 333 231
SacII CC_GC'GG
Cuts at: 1254 1472 1569 1254
Size: 218 97 6611
Fragments arranged by size:
6611 218 97
SalI G'TCGA_C
Cuts at: 1549 3963 1549
Size: 2414 4512
SanDI GG'GwC_CC
Cuts at: 1602 1887 2492 1602
Size: 285 605 6036
Fragments arranged by size:
6036 605 285
SapI GCTCTTCn'nnn_
Cuts at: 4949 5159 4949
Size: 210 6716
SbfI CC_TGCA'GG
Cuts at: 1307 2438 3973 1307
Size: 1131 1535 4260
Fragments arranged by size:
4260 1535 1131
ScaI AGT'ACT
Cuts at: 1387 6443 1387
Size: 5056 1870
SfcI C'TryA_G
Cuts at: 33 271 327 487 886 937 1303 2434 2476
Size: 238 56 160 399 51 366 1131 42
Cuts at: 2476 2977 3330 3969 4654 5622 5813 33
Size: 501 353 639 685 968 191 1146
Fragments arranged by size:
1146 1131 968 685 639 501 399 366
353 238 191 160 56 51 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 1270 1270
Size: 6926
SfoI GGC'GCC
Cuts at: 1005 1757 3371 3573 4358 4606 1005
Size: 752 1614 202 785 248 3325
Fragments arranged by size:
3325 1614 785 752 248 202
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6926
SgrAI Cr'CCGG_yG
Cuts at: 3372 3372
Size: 6926
SmaI CCC'GGG
Cuts at: 1077 1337 2718 2862 2955 3547 3569 3948 1077
Size: 260 1381 144 93 592 22 379 4055
Fragments arranged by size:
4055 1381 592 379 260 144 93 22
SmlI C'TyrA_G
Cuts at: 142 427 865 2650 2800 3430 4081 4996 5710
Size: 285 438 1785 150 630 651 915 714
Cuts at: 5710 5972 142
Size: 262 1096
Fragments arranged by size:
1785 1096 915 714 651 630 438 285
262 150
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6926
SphI G_CATG'C
Cuts at: 3979 4479 3979
Size: 500 6426
SrfI GCCC'GGGC
Cuts at: 1077 1077
Size: 6926
StuI AGG'CCT
Cuts at: 1220 1264 2683 2919 1220
Size: 44 1419 236 5227
Fragments arranged by size:
5227 1419 236 44
StyI C'CwwG_G
Cuts at: 107 464 998 1517 3048 3059 3257 3575 3725
Size: 357 534 519 1531 11 198 318 150
Cuts at: 3725 3740 4060 4414 107
Size: 15 320 354 2619
Fragments arranged by size:
2619 1531 534 519 357 354 320 318
198 150 15 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 660 1219 2379 2867 4509 4642 5101 5310 660
Size: 559 1160 488 1642 133 459 209 2276
Fragments arranged by size:
2276 1642 1160 559 488 459 209 133
TatI w'GTAC_w
Cuts at: 632 1232 1385 6441 632
Size: 600 153 5056 1117
Fragments arranged by size:
5056 1117 600 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1819 1851 3777 3809 6443 6475 1819
Size: 32 1926 32 2634 32 2270
Fragments arranged by size:
2634 2270 1926 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 897 1891 3508 4723 897
Size: 994 1617 1215 3100
Fragments arranged by size:
3100 1617 1215 994
XbaI T'CTAG_A
Cuts at: 3957 3957
Size: 6926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4421 4421
Size: 6926
XhoI C'TCGA_G
Cuts at: 2800 2800
Size: 6926
XmaI C'CCGG_G
Cuts at: 1075 1335 2716 2860 2953 3545 3567 3946 1075
Size: 260 1381 144 93 592 22 379 4055
Fragments arranged by size:
4055 1381 592 379 260 144 93 22
XmnI GAAnn'nnTTC
Cuts at: 214 214
Size: 6926
ZraI GAC'GTC
Cuts at: 1554 3510 1554
Size: 1956 4970
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AvrII BaeI BfrBI BmtI BsiWI BsmI
BsrGI BstZ17I ClaI CspCI DraIII EcoRV FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PsiI
PsrI SexAI SnaBI SspI SwaI