Restriction map for
pF1KA0628
(Circular) (Six-base) MAPSORT of: pF1KA0628.seq Check: 1552 from: 1 to: 4721
pF1KA0628 4721 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 336 336
Size: 4721
AatII G_ACGT'C
Cuts at: 211 1186 211
Size: 975 3746
AccI GT'mk_AC
Cuts at: 1759 1759
Size: 4721
Acc65I G'GTAC_C
Cuts at: 1737 1737
Size: 4721
AclI AA'CG_TT
Cuts at: 1610 4561 1610
Size: 2951 1770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 430 822 1158 1574 2546 2978 3331 3993
Size: 242 392 336 416 972 432 353 662
Cuts at: 3993 188
Size: 916
Fragments arranged by size:
972 916 662 432 416 392 353 336
242
AfeI AGC'GCT
Cuts at: 1171 1171
Size: 4721
AflIII A'CryG_T
Cuts at: 560 2073 3873 4154 560
Size: 1513 1800 281 1127
Fragments arranged by size:
1800 1513 1127 281
AhdI GACnn_n'nnGTC
Cuts at: 643 643
Size: 4721
AleI CACnn'nnGTG
Cuts at: 967 967
Size: 4721
AlwNI CAG_nnn'CTG
Cuts at: 309 338 2026 3464 309
Size: 29 1688 1438 1566
Fragments arranged by size:
1688 1566 1438 29
ApaLI G'TGCA_C
Cuts at: 396 3559 396
Size: 3163 1558
ApoI r'AATT_y
Cuts at: 620 1040 1208 1544 1725 4074 4330 620
Size: 420 168 336 181 2349 256 1011
Fragments arranged by size:
2349 1011 420 336 256 181 168
AseI AT'TA_AT
Cuts at: 20 20
Size: 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 244 933 1741 244
Size: 689 808 3224
Fragments arranged by size:
3224 808 689
BamHI G'GATC_C
Cuts at: 1124 1746 1124
Size: 622 4099
BanI G'GyrC_C
Cuts at: 779 1737 2151 2399 2434 779
Size: 958 414 248 35 3066
Fragments arranged by size:
3066 958 414 248 35
BanII G_rGCy'C
Cuts at: 236 269 284 458 598 850 934 1438 1735
Size: 33 15 174 140 252 84 504 297
Cuts at: 1735 2765 236
Size: 1030 2192
Fragments arranged by size:
2192 1030 504 297 252 174 140 84
33 15
BbeI G_GCGC'C
Cuts at: 2155 2403 2155
Size: 248 4473
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3675 4721 2619
Size: 1056 1046 2619
Fragments arranged by size:
2619 1056 1046
BclI T'GATC_A
Cuts at: 2241 2241
Size: 4721
BglII A'GATC_T
Cuts at: 2236 4273 2236
Size: 2037 2684
BlpI GC'TnA_GC
Cuts at: 1833 1833
Size: 4721
Bme1580I G_kGCm'C
Cuts at: 400 2346 2439 3563 400
Size: 1946 93 1124 1558
Fragments arranged by size:
1946 1558 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1142 1933 2014 2349 4493 1142
Size: 791 81 335 2144 1370
Fragments arranged by size:
2144 1370 791 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 655 1243 1662 3088 255
Size: 400 588 419 1426 1888
Fragments arranged by size:
1888 1426 588 419 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1238 1322 1330 1490 650
Size: 588 84 8 160 3881
Fragments arranged by size:
3881 588 160 84 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1671 1897 2776 3490 3788 374
Size: 1297 226 879 714 298 1307
Fragments arranged by size:
1307 1297 879 714 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 691 798 1050 4425 691
Size: 107 252 3375 987
Fragments arranged by size:
3375 987 252 107
BsaAI yAC'GTr
Cuts at: 563 1149 2074 563
Size: 586 925 3210
Fragments arranged by size:
3210 925 586
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 208 1183 2152 2400 3102 4598 208
Size: 975 969 248 702 1496 331
Fragments arranged by size:
1496 975 969 702 331 248
BsaWI w'CCGG_w
Cuts at: 1048 1910 2431 3109 3520 3667 3941 1048
Size: 862 521 678 411 147 274 1828
Fragments arranged by size:
1828 862 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1295 1325 1295
Size: 30 4691
BseYI C'CCAG_C
Cuts at: 544 1196 2146 2904 3569 3977 544
Size: 652 950 758 665 408 1288
Fragments arranged by size:
1288 950 758 665 652 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 1509 1435
Size: 74 4647
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3539 104
Size: 2159 46 1230 1286
Fragments arranged by size:
2159 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 236 284 400 458 598 850 934 1370 1438
Size: 48 116 58 140 252 84 436 68
Cuts at: 1438 1735 2513 2703 3563 236
Size: 297 778 190 860 1394
Fragments arranged by size:
1394 860 778 436 297 252 190 140
116 84 68 58 48
BsmI GAATG_Cn'
Cuts at: 820 988 1003 1072 820
Size: 168 15 69 4469
Fragments arranged by size:
4469 168 69 15
Bsp1286I G_dGCh'C
Cuts at: 236 269 284 400 458 598 850 934 1370
Size: 33 15 116 58 140 252 84 436
Cuts at: 1370 1438 1735 2346 2439 2513 2703 2765 3563
Size: 68 297 611 93 74 190 62 798
Cuts at: 3563 236
Size: 1394
Fragments arranged by size:
1394 798 611 436 297 252 190 140
116 93 84 74 68 62 58 33
15
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 1771 1985 2287 2668 336
Size: 1435 214 302 381 2389
Fragments arranged by size:
2389 1435 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3013 3067 4551 4716 50
Size: 2963 54 1484 165 55
Fragments arranged by size:
2963 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 909 1077 1161 2633 909
Size: 168 84 1472 2997
Fragments arranged by size:
2997 1472 168 84
BsrFI r'CCGG_y
Cuts at: 2719 2900 2719
Size: 181 4540
BssSI C'ACGA_G
Cuts at: 262 2992 3700 262
Size: 2730 708 1283
Fragments arranged by size:
2730 1283 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2098 2098
Size: 4721
BstBI TT'CG_AA
Cuts at: 1400 3082 1400
Size: 1682 3039
BstXI CCAn_nnnn'nTGG
Cuts at: 1671 1671
Size: 4721
BstYI r'GATC_y
Cuts at: 1 368 956 1124 1376 1746 2213 2236 2571
Size: 367 588 168 252 370 467 23 335
Cuts at: 2571 2817 3123 3135 3221 4273 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 588 467 449 370 367 335 306
252 246 168 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 997 2671 2852 3015 997
Size: 1674 181 163 2703
Fragments arranged by size:
2703 1674 181 163
BtsI GCAGTG_nn'
Cuts at: 745 1964 745
Size: 1219 3502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 807 842 891 926 975 1010 1059 1094 1143
Size: 35 49 35 49 35 49 35 49
Cuts at: 1143 1178 807
Size: 35 4350
Fragments arranged by size:
4350 49 49 49 49 35 35 35
35 35
DraI TTT'AAA
Cuts at: 1720 1720
Size: 4721
DrdI GACnn_nn'nnGTC
Cuts at: 2427 3771 2427
Size: 1344 3377
EaeI y'GGCC_r
Cuts at: 588 2306 2480 2871 2898 4243 588
Size: 1718 174 391 27 1345 1066
Fragments arranged by size:
1718 1345 1066 391 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 1550 2744 2954 1550
Size: 1194 210 3317
Fragments arranged by size:
3317 1194 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2929 3671 3817 4531 2929
Size: 742 146 714 3119
Fragments arranged by size:
3119 742 714 146
EcoICRI GAG'CTC
Cuts at: 234 282 456 596 848 932 1436 1733 234
Size: 48 174 140 252 84 504 297 3222
Fragments arranged by size:
3222 504 297 252 174 140 84 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 430 650 822 1158 1238 1322 1330 1490
Size: 242 220 172 336 80 84 8 160
Cuts at: 1490 1574 2546 2978 3331 3993 188
Size: 84 972 432 353 662 916
Fragments arranged by size:
972 916 662 432 353 336 242 220
172 160 84 84 80 8
EcoNI CCTnn'n_nnAGG
Cuts at: 924 924
Size: 4721
EcoO109I rG'GnC_Cy
Cuts at: 890 1394 1659 1860 890
Size: 504 265 201 3751
Fragments arranged by size:
3751 504 265 201
EcoRI G'AATT_C
Cuts at: 620 1040 1208 1725 620
Size: 420 168 517 3616
Fragments arranged by size:
3616 517 420 168
EcoRV GAT'ATC
Cuts at: 486 486
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4721
FspI TGC'GCA
Cuts at: 737 2502 737
Size: 1765 2956
HaeII r_GCGC'y
Cuts at: 1173 2155 2403 3633 4404 1173
Size: 982 248 1230 771 1490
Fragments arranged by size:
1490 1230 982 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 629 661 1582 1614 2563 2593 2595
Size: 32 419 32 921 32 949 30 2
Cuts at: 2595 2625 178
Size: 30 2274
Fragments arranged by size:
2274 949 921 419 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1760 1760
Size: 4721
HindIII A'AGCT_T
Cuts at: 1253 1253
Size: 4721
KasI G'GCGC_C
Cuts at: 2151 2399 2151
Size: 248 4473
KpnI G_GTAC'C
Cuts at: 1741 1741
Size: 4721
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3480 3664 3480
Size: 184 4537
MscI TGG'CCA
Cuts at: 590 2482 590
Size: 1892 2829
MslI CAynn'nnrTG
Cuts at: 226 967 1673 2710 2837 226
Size: 741 706 1037 127 2110
Fragments arranged by size:
2110 1037 741 706 127
MspA1I CmG'CkG
Cuts at: 1832 2146 2506 3290 3535 1832
Size: 314 360 784 245 3018
Fragments arranged by size:
3018 784 360 314 245
NaeI GCC'GGC
Cuts at: 2902 2902
Size: 4721
NarI GG'CG_CC
Cuts at: 2152 2400 2152
Size: 248 4473
NgoMIV G'CCGG_C
Cuts at: 2900 2900
Size: 4721
NspI r_CATG'y
Cuts at: 544 1774 2274 3877 544
Size: 1230 500 1603 1388
Fragments arranged by size:
1603 1388 1230 500
PciI A'CATG_T
Cuts at: 3873 3873
Size: 4721
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PmlI CAC'GTG
Cuts at: 563 563
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 4689
PspXI vC'TCGA_Gb
Cuts at: 933 933
Size: 4721
PstI C_TGCA'G
Cuts at: 1768 2453 1768
Size: 685 4036
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 2146 2506 2146
Size: 360 4361
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 236 284 458 598 850 934 1438 1735 236
Size: 48 174 140 252 84 504 297 3222
Fragments arranged by size:
3222 504 297 252 174 140 84 48
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SfcI C'TryA_G
Cuts at: 33 1685 1764 2449 3417 3608 33
Size: 1652 79 685 968 191 1146
Fragments arranged by size:
1652 1146 968 685 191 79
SfoI GGC'GCC
Cuts at: 2153 2401 2153
Size: 248 4473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 1743 1743
Size: 4721
SmlI C'TyrA_G
Cuts at: 389 933 1650 1876 2791 3505 3767 389
Size: 544 717 226 915 714 262 1343
Fragments arranged by size:
1343 915 717 714 544 262 226
SnaBI TAC'GTA
Cuts at: 1149 1149
Size: 4721
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 544 1774 2274 544
Size: 1230 500 2991
Fragments arranged by size:
2991 1230 500
StuI AGG'CCT
Cuts at: 1143 1143
Size: 4721
StyI C'CwwG_G
Cuts at: 1855 2209 1855
Size: 354 4367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1210 1663 2304 2437 2896 3105 1210
Size: 453 641 133 459 209 2826
Fragments arranged by size:
2826 641 459 453 209 133
TatI w'GTAC_w
Cuts at: 4236 4236
Size: 4721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1295 1327 4238 4270 1295
Size: 32 2911 32 1746
Fragments arranged by size:
2911 1746 32 32
Tth111I GACn'n_nGTC
Cuts at: 207 2518 207
Size: 2311 2410
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2216 2216
Size: 4721
XhoI C'TCGA_G
Cuts at: 933 933
Size: 4721
XmaI C'CCGG_G
Cuts at: 1741 1741
Size: 4721
XmnI GAAnn'nnTTC
Cuts at: 667 835 919 1003 1171 1255 1423 667
Size: 168 84 84 168 84 168 3965
Fragments arranged by size:
3965 168 168 168 84 84 84
ZraI GAC'GTC
Cuts at: 209 1184 209
Size: 975 3746
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PmlI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AlfI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI DraIII FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI SwaI