Restriction map for
pF1KA0623
(Circular) (Six-base) MAPSORT of: pF1KA0623.seq Check: 9658 from: 1 to: 6221
pF1KA0623 6221 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2157 3259 2157
Size: 1102 5119
Acc65I G'GTAC_C
Cuts at: 1552 3237 1552
Size: 1685 4536
AclI AA'CG_TT
Cuts at: 6061 6061
Size: 6221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 847 2015 4046 4478 5225 5493 280
Size: 567 1168 2031 432 747 268 1008
Fragments arranged by size:
2031 1168 1008 747 567 432 268
AflII C'TTAA_G
Cuts at: 771 771
Size: 6221
AflIII A'CryG_T
Cuts at: 2330 3573 4677 5654 2330
Size: 1243 1104 977 2897
Fragments arranged by size:
2897 1243 1104 977
AgeI A'CCGG_T
Cuts at: 4609 4609
Size: 6221
AleI CACnn'nnGTG
Cuts at: 1202 2854 1202
Size: 1652 4569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 467 2923 467
Size: 2456 3765
AlwNI CAG_nnn'CTG
Cuts at: 3526 5093 3526
Size: 1567 4654
ApaI G_GGCC'C
Cuts at: 2366 2366
Size: 6221
ApaLI G'TGCA_C
Cuts at: 2667 4991 2667
Size: 2324 3897
ApoI r'AATT_y
Cuts at: 1246 2893 3225 5574 5830 1246
Size: 1647 332 2349 256 1637
Fragments arranged by size:
2349 1647 1637 332 256
AseI AT'TA_AT
Cuts at: 20 230 20
Size: 210 6011
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
AvaI C'yCGr_G
Cuts at: 3241 3241
Size: 6221
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1405 1438 1405
Size: 33 6188
BamHI G'GATC_C
Cuts at: 634 3246 634
Size: 2612 3609
BanI G'GyrC_C
Cuts at: 188 1173 1552 2238 3237 3651 3899 3934 188
Size: 985 379 686 999 414 248 35 2475
Fragments arranged by size:
2475 999 985 686 414 379 248 35
BanII G_rGCy'C
Cuts at: 2366 2383 2522 2845 3235 4265 2366
Size: 17 139 323 390 1030 4322
Fragments arranged by size:
4322 1030 390 323 139 17
BbeI G_GCGC'C
Cuts at: 3655 3903 3655
Size: 248 5973
BbsI GAAGACnn'nnnn_
Cuts at: 168 428 1511 3186 168
Size: 260 1083 1675 3203
Fragments arranged by size:
3203 1675 1083 260
BbvCI CC'TCA_GC
Cuts at: 1796 1796
Size: 6221
BciVI GTATCCnnnnn_n'
Cuts at: 4119 4880 6221 4119
Size: 761 1341 4119
Fragments arranged by size:
4119 1341 761
BclI T'GATC_A
Cuts at: 3741 3741
Size: 6221
BglI GCCn_nnn'nGGC
Cuts at: 1439 1806 1439
Size: 367 5854
BglII A'GATC_T
Cuts at: 498 1402 2104 3736 5773 498
Size: 904 702 1632 2037 946
Fragments arranged by size:
2037 1632 946 904 702
BlpI GC'TnA_GC
Cuts at: 2731 3333 2731
Size: 602 5619
Bme1580I G_kGCm'C
Cuts at: 962 1762 2037 2366 2671 3846 3939 4995 962
Size: 800 275 329 305 1175 93 1056 2188
Fragments arranged by size:
2188 1175 1056 800 329 305 275 93
BmgBI CAC'GTC
Cuts at: 1234 1234
Size: 6221
BmrI ACTGGGnnnn_n'
Cuts at: 943 1462 1523 1781 1796 2751 3433 3514 3849
Size: 519 61 258 15 955 682 81 335
Cuts at: 3849 5993 943
Size: 2144 1171
Fragments arranged by size:
2144 1171 955 682 519 335 258 81
61 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4588 4588
Size: 6221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1593 1619 2435 1593
Size: 26 816 5379
Fragments arranged by size:
5379 816 26
Bpu10I CC'TnA_GC
Cuts at: 686 1374 1562 1796 2660 686
Size: 688 188 234 864 4247
Fragments arranged by size:
4247 864 688 234 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 940 1931 3397 4276 4768 5066 749
Size: 191 991 1466 879 492 298 1904
Fragments arranged by size:
1904 1466 991 879 492 298 191
BsaI GGTCTCn'nnnn_
Cuts at: 374 2297 5925 374
Size: 1923 3628 670
Fragments arranged by size:
3628 1923 670
BsaAI yAC'GTr
Cuts at: 2484 3574 2484
Size: 1090 5131
BsaBI GATnn'nnATC
Cuts at: 6 3735 5772 6
Size: 3729 2037 455
Fragments arranged by size:
3729 2037 455
BsaHI Gr'CG_yC
Cuts at: 3652 3900 4602 6098 3652
Size: 248 702 1496 3775
Fragments arranged by size:
3775 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3410 3931 4609 4883 5030 5441 3410
Size: 521 678 274 147 411 4190
Fragments arranged by size:
4190 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1908 1938 1908
Size: 30 6191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1614 2285 2566 1614
Size: 671 281 5269
Fragments arranged by size:
5269 671 281
BseYI C'CCAG_C
Cuts at: 1777 2365 2470 2506 3646 4404 4981 5477 1777
Size: 588 105 36 1140 758 577 496 2521
Fragments arranged by size:
2521 1140 758 588 577 496 105 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 457
Size: 6221
BsiEI CG_ry'CG
Cuts at: 104 1453 3763 3809 5017 104
Size: 1349 2310 46 1208 1308
Fragments arranged by size:
2310 1349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2671 3235 4013 4203 4995 2671
Size: 564 778 190 792 3897
Fragments arranged by size:
3897 792 778 564 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2548 2548
Size: 6221
Bsp1286I G_dGCh'C
Cuts at: 962 1762 2037 2366 2383 2522 2671 2845 3235
Size: 800 275 329 17 139 149 174 390
Cuts at: 3235 3846 3939 4013 4203 4265 4995 962
Size: 611 93 74 190 62 730 2188
Fragments arranged by size:
2188 800 730 611 390 329 275 190
174 149 139 93 74 62 17
BspEI T'CCGG_A
Cuts at: 5441 5441
Size: 6221
BspHI T'CATG_A
Cuts at: 5397 5397
Size: 6221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2239 3271 3485 3787 4168 2239
Size: 1032 214 302 381 4292
Fragments arranged by size:
4292 1032 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2568 4513 4567 6051 6216 50
Size: 2518 1945 54 1484 165 55
Fragments arranged by size:
2518 1945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 378 2037 4133 378
Size: 1659 2096 2466
Fragments arranged by size:
2466 2096 1659
BsrFI r'CCGG_y
Cuts at: 4219 4400 4609 4219
Size: 181 209 5831
Fragments arranged by size:
5831 209 181
BsrGI T'GTAC_A
Cuts at: 644 1386 2768 644
Size: 742 1382 4097
Fragments arranged by size:
4097 1382 742
BssSI C'ACGA_G
Cuts at: 148 4492 4850 148
Size: 4344 358 1519
Fragments arranged by size:
4344 1519 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2245 3598 2245
Size: 1353 4868
BstBI TT'CG_AA
Cuts at: 2340 4582 2340
Size: 2242 3979
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1437 1842 114
Size: 1323 405 4493
Fragments arranged by size:
4493 1323 405
BstYI r'GATC_y
Cuts at: 1 144 498 634 1402 2104 2693 3246 3713
Size: 143 354 136 768 702 589 553 467
Cuts at: 3713 3736 4071 4317 5329 5415 5427 5773 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 768 702 589 553 467 449 354
346 335 246 143 136 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 656 1595 1626 656
Size: 939 31 5251
Fragments arranged by size:
5251 939 31
BtgI C'CryG_G
Cuts at: 107 170 1436 1855 2023 3212 107
Size: 63 1266 419 168 1189 3116
Fragments arranged by size:
3116 1266 1189 419 168 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4171 4352 4515 4171
Size: 181 163 5877
Fragments arranged by size:
5877 181 163
BtsI GCAGTG_nn'
Cuts at: 1273 1415 2099 3464 1273
Size: 142 684 1365 4030
Fragments arranged by size:
4030 1365 684 142
ClaI AT'CG_AT
Cuts at: 2937 2937
Size: 6221
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2339 2374 2339
Size: 35 6186
DraI TTT'AAA
Cuts at: 1878 2183 3220 1878
Size: 305 1037 4879
Fragments arranged by size:
4879 1037 305
DraIII CAC_nnn'GTG
Cuts at: 631 2305 2621 631
Size: 1674 316 4231
Fragments arranged by size:
4231 1674 316
DrdI GACnn_nn'nnGTC
Cuts at: 3927 4785 3927
Size: 858 5363
EaeI y'GGCC_r
Cuts at: 1450 2400 3806 3980 4371 4398 5743 1450
Size: 950 1406 174 391 27 1345 1928
Fragments arranged by size:
1928 1406 1345 950 391 174 27
EagI C'GGCC_G
Cuts at: 1450 3806 1450
Size: 2356 3865
EarI CTCTTCn'nnn_
Cuts at: 1149 2284 2448 4244 4454 1149
Size: 1135 164 1796 210 2916
Fragments arranged by size:
2916 1796 1135 210 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4429 4739 4885 6031 4429
Size: 310 146 1146 4619
Fragments arranged by size:
4619 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3233 3233
Size: 6221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 847 1593 1619 2015 2435 4046 4478 5225
Size: 567 746 26 396 420 1611 432 747
Cuts at: 5225 5493 280
Size: 268 1008
Fragments arranged by size:
1611 1008 747 746 567 432 420 396
268 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 2429 2246
Size: 183 6038
EcoO109I rG'GnC_Cy
Cuts at: 161 1087 1847 2362 2417 3129 3360 161
Size: 926 760 515 55 712 231 3022
Fragments arranged by size:
3022 926 760 712 515 231 55
EcoRI G'AATT_C
Cuts at: 1246 3225 1246
Size: 1979 4242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 711 4240 711
Size: 3529 2692
FspI TGC'GCA
Cuts at: 2911 4002 2911
Size: 1091 5130
HaeII r_GCGC'y
Cuts at: 1694 3193 3655 3903 4925 5904 1694
Size: 1499 462 248 1022 979 2011
Fragments arranged by size:
2011 1499 1022 979 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1490 1522 1908 1940 4063 4093 4095 4125 1490
Size: 32 386 32 2123 30 2 30 3586
Fragments arranged by size:
3586 2123 386 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2158 3260 2158
Size: 1102 5119
KasI G'GCGC_C
Cuts at: 3651 3899 3651
Size: 248 5973
KpnI G_GTAC'C
Cuts at: 1556 3241 1556
Size: 1685 4536
MluI A'CGCG_T
Cuts at: 5654 5654
Size: 6221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 714 1448 1476 1982 4892 5076 714
Size: 734 28 506 2910 184 1859
Fragments arranged by size:
2910 1859 734 506 184 28
MscI TGG'CCA
Cuts at: 3982 3982
Size: 6221
MslI CAynn'nnrTG
Cuts at: 112 680 1202 2028 2597 2854 2905 4210 4337
Size: 568 522 826 569 257 51 1305 127
Cuts at: 4337 112
Size: 1996
Fragments arranged by size:
1996 1305 826 569 568 522 257 127
51
MspA1I CmG'CkG
Cuts at: 2070 2730 2760 2787 2854 3332 3646 4006 5019
Size: 660 30 27 67 478 314 360 1013
Cuts at: 5019 5264 2070
Size: 245 3027
Fragments arranged by size:
3027 1013 660 478 360 314 245 67
30 27
NaeI GCC'GGC
Cuts at: 4402 4402
Size: 6221
NarI GG'CG_CC
Cuts at: 3652 3900 3652
Size: 248 5973
NcoI C'CATG_G
Cuts at: 107 1436 107
Size: 1329 4892
NgoMIV G'CCGG_C
Cuts at: 4400 4400
Size: 6221
NspI r_CATG'y
Cuts at: 2334 3274 3774 4681 2334
Size: 940 500 907 3874
Fragments arranged by size:
3874 940 907 500
PciI A'CATG_T
Cuts at: 2330 4677 2330
Size: 2347 3874
PfoI T'CCnGG_A
Cuts at: 1609 2451 2696 2830 1609
Size: 842 245 134 5000
Fragments arranged by size:
5000 842 245 134
PmeI GTTT'AAAC
Cuts at: 3220 3220
Size: 6221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5360 5392 5360
Size: 32 6189
PpuMI rG'GwC_Cy
Cuts at: 1087 1847 2417 3129 1087
Size: 760 570 712 4179
Fragments arranged by size:
4179 760 712 570
PspOMI G'GGCC_C
Cuts at: 2362 2362
Size: 6221
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 6189
PstI C_TGCA'G
Cuts at: 1410 1688 2250 2991 3036 3138 3268 3953 1410
Size: 278 562 741 45 102 130 685 3678
Fragments arranged by size:
3678 741 685 562 278 130 102 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6221
PvuII CAG'CTG
Cuts at: 2730 2760 2787 2854 3646 4006 2730
Size: 30 27 67 792 360 4945
Fragments arranged by size:
4945 792 360 67 30 27
RsrII CG'GwC_CG
Cuts at: 4416 4416
Size: 6221
SacI G_AGCT'C
Cuts at: 3235 3235
Size: 6221
SalI G'TCGA_C
Cuts at: 2156 3258 2156
Size: 1102 5119
SanDI GG'GwC_CC
Cuts at: 2417 2417
Size: 6221
SapI GCTCTTCn'nnn_
Cuts at: 2448 4244 4454 2448
Size: 1796 210 4215
Fragments arranged by size:
4215 1796 210
SbfI CC_TGCA'GG
Cuts at: 2250 3268 2250
Size: 1018 5203
ScaI AGT'ACT
Cuts at: 1128 5738 1128
Size: 4610 1611
SfcI C'TryA_G
Cuts at: 33 1406 1684 2246 2285 2987 3014 3032 3124
Size: 1373 278 562 39 702 27 18 92
Cuts at: 3124 3134 3264 3949 4942 5133 33
Size: 10 130 685 993 191 1121
Fragments arranged by size:
1373 1121 993 702 685 562 278 191
130 92 39 27 18 10
SfoI GGC'GCC
Cuts at: 3653 3901 3653
Size: 248 5973
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
SmaI CCC'GGG
Cuts at: 3243 3243
Size: 6221
SmlI C'TyrA_G
Cuts at: 764 771 919 1946 3376 4291 4783 5045 764
Size: 7 148 1027 1430 915 492 262 1940
Fragments arranged by size:
1940 1430 1027 915 492 262 148 7
SpeI A'CTAG_T
Cuts at: 67 1129 67
Size: 1062 5159
SphI G_CATG'C
Cuts at: 3274 3774 3274
Size: 500 5721
SspI AAT'ATT
Cuts at: 309 309
Size: 6221
StuI AGG'CCT
Cuts at: 1527 3110 1527
Size: 1583 4638
StyI C'CwwG_G
Cuts at: 107 1436 1818 1970 2267 3355 3709 107
Size: 1329 382 152 297 1088 354 2619
Fragments arranged by size:
2619 1329 1088 382 354 297 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 109 3076 3804 3937 4396 4605 109
Size: 2967 728 133 459 209 1725
Fragments arranged by size:
2967 1725 728 459 209 133
TatI w'GTAC_w
Cuts at: 131 644 1126 1386 2768 5736 131
Size: 513 482 260 1382 2968 616
Fragments arranged by size:
2968 1382 616 513 482 260
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 402 434 1831 1863 5738 5770 402
Size: 32 1397 32 3875 32 853
Fragments arranged by size:
3875 1397 853 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4018 4018
Size: 6221
XbaI T'CTAG_A
Cuts at: 3252 3252
Size: 6221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 730 1774 1839 3716 114
Size: 616 1044 65 1877 2619
Fragments arranged by size:
2619 1877 1044 616 65
XmaI C'CCGG_G
Cuts at: 3241 3241
Size: 6221
XmnI GAAnn'nnTTC
Cuts at: 2938 3023 2938
Size: 85 6136
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI AscI AvrII BcgI
BfrBI BmtI BsiWI BsmI BssHII BstEII BstZ17I EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI