Restriction map for
pF1KA0611
(Circular) (Six-base) MAPSORT of: pF1KA0611.seq Check: 1750 from: 1 to: 5846
pF1KA0611 5846 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 2069 2069
Size: 5846
AccI GT'mk_AC
Cuts at: 476 1181 2884 476
Size: 705 1703 3438
Fragments arranged by size:
3438 1703 705
Acc65I G'GTAC_C
Cuts at: 683 2862 683
Size: 2179 3667
AclI AA'CG_TT
Cuts at: 5686 5686
Size: 5846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 1999 3671 4103 4850 5118 646
Size: 1353 1672 432 747 268 1374
Fragments arranged by size:
1672 1374 1353 747 432 268
AflIII A'CryG_T
Cuts at: 3198 4302 5279 3198
Size: 1104 977 3765
Fragments arranged by size:
3765 1104 977
AgeI A'CCGG_T
Cuts at: 4234 4234
Size: 5846
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1081 1378 1889 1081
Size: 297 511 5038
Fragments arranged by size:
5038 511 297
AlwNI CAG_nnn'CTG
Cuts at: 1299 3151 4718 1299
Size: 1852 1567 2427
Fragments arranged by size:
2427 1852 1567
ApaI G_GGCC'C
Cuts at: 1024 1024
Size: 5846
ApaLI G'TGCA_C
Cuts at: 362 1066 1573 1755 2551 4616 362
Size: 704 507 182 796 2065 1592
Fragments arranged by size:
2065 1592 796 704 507 182
ApoI r'AATT_y
Cuts at: 2850 5199 5455 2850
Size: 2349 256 3241
Fragments arranged by size:
3241 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5846
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
AvaI C'yCGr_G
Cuts at: 199 805 1475 1840 1887 2866 199
Size: 606 670 365 47 979 3179
Fragments arranged by size:
3179 979 670 606 365 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 491 524 491
Size: 33 5813
BamHI G'GATC_C
Cuts at: 2871 2871
Size: 5846
BanI G'GyrC_C
Cuts at: 194 625 683 2862 3276 3524 3559 194
Size: 431 58 2179 414 248 35 2481
Fragments arranged by size:
2481 2179 431 414 248 58 35
BanII G_rGCy'C
Cuts at: 1024 2426 2860 3890 1024
Size: 1402 434 1030 2980
Fragments arranged by size:
2980 1402 1030 434
BbeI G_GCGC'C
Cuts at: 3280 3528 3280
Size: 248 5598
BbsI GAAGACnn'nnnn_
Cuts at: 1172 1256 2808 1172
Size: 84 1552 4210
Fragments arranged by size:
4210 1552 84
BbvCI CC'TCA_GC
Cuts at: 1145 1276 2224 1145
Size: 131 948 4767
Fragments arranged by size:
4767 948 131
BciVI GTATCCnnnnn_n'
Cuts at: 230 2426 3744 4505 5846 230
Size: 2196 1318 761 1341 230
Fragments arranged by size:
2196 1341 1318 761 230
BclI T'GATC_A
Cuts at: 1137 3366 1137
Size: 2229 3617
BglI GCCn_nnn'nGGC
Cuts at: 1081 1646 1081
Size: 565 5281
BglII A'GATC_T
Cuts at: 1319 3361 5398 1319
Size: 2042 2037 1767
Fragments arranged by size:
2042 2037 1767
BlpI GC'TnA_GC
Cuts at: 2656 2958 2656
Size: 302 5544
Bme1580I G_kGCm'C
Cuts at: 197 366 630 1024 1070 1577 1759 1878 1957
Size: 169 264 394 46 507 182 119 79
Cuts at: 1957 1983 2555 3471 3564 4620 197
Size: 26 572 916 93 1056 1423
Fragments arranged by size:
1423 1056 916 572 507 394 264 182
169 119 93 79 46 26
BmrI ACTGGGnnnn_n'
Cuts at: 387 3058 3139 3474 5618 387
Size: 2671 81 335 2144 615
Fragments arranged by size:
2671 2144 615 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 239 4213 239
Size: 3974 1872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 807 1400 1636 1708 1762 1921 807
Size: 593 236 72 54 159 4732
Fragments arranged by size:
4732 593 236 159 72 54
Bpu10I CC'TnA_GC
Cuts at: 836 1145 1276 2224 2420 836
Size: 309 131 948 196 4262
Fragments arranged by size:
4262 948 309 196 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1897 2425 2773 3022 3901 4393 4691 1897
Size: 528 348 249 879 492 298 3052
Fragments arranged by size:
3052 879 528 492 348 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 203 645 1250 1684 5550 203
Size: 442 605 434 3866 499
Fragments arranged by size:
3866 605 499 442 434
BsaAI yAC'GTr
Cuts at: 447 1754 3199 447
Size: 1307 1445 3094
Fragments arranged by size:
3094 1445 1307
BsaBI GATnn'nnATC
Cuts at: 6 2491 3360 5397 6
Size: 2485 869 2037 455
Fragments arranged by size:
2485 2037 869 455
BsaHI Gr'CG_yC
Cuts at: 2066 3277 3525 4227 5723 2066
Size: 1211 248 702 1496 2189
Fragments arranged by size:
2189 1496 1211 702 248
BsaWI w'CCGG_w
Cuts at: 539 3035 3556 4234 4508 4655 5066 539
Size: 2496 521 678 274 147 411 1319
Fragments arranged by size:
2496 1319 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1153 1183 1397 1427 1444 1474 1567 1597 2238
Size: 30 214 30 17 30 93 30 641
Cuts at: 2238 2268 2284 2314 2662 2692 1153
Size: 30 16 30 348 30 4307
Fragments arranged by size:
4307 641 348 214 93 30 30 30
30 30 30 30 17 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 697 1537 445
Size: 252 840 4754
Fragments arranged by size:
4754 840 252
BseYI C'CCAG_C
Cuts at: 189 776 843 989 2818 3271 4029 4606 5102
Size: 587 67 146 1829 453 758 577 496
Cuts at: 5102 189
Size: 933
Fragments arranged by size:
1829 933 758 587 577 496 453 146
67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 2063 1453
Size: 610 5236
BsiEI CG_ry'CG
Cuts at: 104 1581 1961 2452 3388 3434 4642 104
Size: 1477 380 491 936 46 1208 1308
Fragments arranged by size:
1477 1308 1208 936 491 380 46
BsiHKAI G_wGCw'C
Cuts at: 366 1070 1496 1577 1759 2426 2509 2555 2860
Size: 704 426 81 182 667 83 46 305
Cuts at: 2860 3638 3828 4620 366
Size: 778 190 792 1592
Fragments arranged by size:
1592 792 778 704 667 426 305 190
182 83 81 46
BsmI GAATG_Cn'
Cuts at: 1707 1773 1707
Size: 66 5780
Bsp1286I G_dGCh'C
Cuts at: 197 366 630 1024 1070 1496 1577 1759 1878
Size: 169 264 394 46 426 81 182 119
Cuts at: 1878 1957 1983 2426 2509 2555 2860 3471 3564
Size: 79 26 443 83 46 305 611 93
Cuts at: 3564 3638 3828 3890 4620 197
Size: 74 190 62 730 1423
Fragments arranged by size:
1423 730 611 443 426 394 305 264
190 182 169 119 93 83 81 79
74 62 46 46 26
BspEI T'CCGG_A
Cuts at: 539 5066 539
Size: 4527 1319
BspHI T'CATG_A
Cuts at: 548 2636 5022 548
Size: 2088 2386 1372
Fragments arranged by size:
2386 2088 1372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 695 2804 2896 3110 3412 3793 695
Size: 2109 92 214 302 381 2748
Fragments arranged by size:
2748 2109 381 302 214 92
BsrBI CCG'CTC
Cuts at: 50 1583 4138 4192 5676 5841 50
Size: 1533 2555 54 1484 165 55
Fragments arranged by size:
2555 1533 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2067 3758 2067
Size: 1691 4155
BsrFI r'CCGG_y
Cuts at: 482 1008 2174 3844 4025 4234 482
Size: 526 1166 1670 181 209 2094
Fragments arranged by size:
2094 1670 1166 526 209 181
BsrGI T'GTAC_A
Cuts at: 770 770
Size: 5846
BssSI C'ACGA_G
Cuts at: 494 1495 2695 4117 4475 494
Size: 1001 1200 1422 358 1865
Fragments arranged by size:
1865 1422 1200 1001 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3223 3223
Size: 5846
BstBI TT'CG_AA
Cuts at: 786 4207 786
Size: 3421 2425
BstEII G'GTnAC_C
Cuts at: 1779 1815 1779
Size: 36 5810
BstYI r'GATC_y
Cuts at: 1 434 614 1295 1319 2433 2871 3338 3361
Size: 433 180 681 24 1114 438 467 23
Cuts at: 3361 3696 3942 4954 5040 5052 5398 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1114 1012 681 467 449 438 433 346
335 246 180 86 24 23 12
BstZ17I GTA'TAC
Cuts at: 1182 1182
Size: 5846
Bsu36I CC'TnA_GG
Cuts at: 1696 1696
Size: 5846
BtgI C'CryG_G
Cuts at: 107 812 1598 1824 107
Size: 705 786 226 4129
Fragments arranged by size:
4129 786 705 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1071 2658 2703 3796 3977 4140 1071
Size: 1587 45 1093 181 163 2777
Fragments arranged by size:
2777 1587 1093 181 163 45
BtsI GCAGTG_nn'
Cuts at: 3089 3089
Size: 5846
ClaI AT'CG_AT
Cuts at: 2705 2705
Size: 5846
DraI TTT'AAA
Cuts at: 2845 2845
Size: 5846
DraIII CAC_nnn'GTG
Cuts at: 1104 2559 1104
Size: 1455 4391
DrdI GACnn_nn'nnGTC
Cuts at: 460 2389 3552 4410 460
Size: 1929 1163 858 1896
Fragments arranged by size:
1929 1896 1163 858
EaeI y'GGCC_r
Cuts at: 401 1595 1958 2172 2193 2449 2561 3431 3605
Size: 1194 363 214 21 256 112 870 174
Cuts at: 3605 3996 4023 5368 401
Size: 391 27 1345 879
Fragments arranged by size:
1345 1194 879 870 391 363 256 214
174 112 27 21
EagI C'GGCC_G
Cuts at: 1958 2449 3431 1958
Size: 491 982 4373
Fragments arranged by size:
4373 982 491
EarI CTCTTCn'nnn_
Cuts at: 199 1451 3869 4079 199
Size: 1252 2418 210 1966
Fragments arranged by size:
2418 1966 1252 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1062 2662 4054 4364 4510 5656 1062
Size: 1600 1392 310 146 1146 1252
Fragments arranged by size:
1600 1392 1252 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2424 2858 2424
Size: 434 5412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 807 1400 1636 1708 1762 1921 1999 3671
Size: 161 593 236 72 54 159 78 1672
Cuts at: 3671 4103 4850 5118 646
Size: 432 747 268 1374
Fragments arranged by size:
1672 1374 747 593 432 268 236 161
159 78 72 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1857 2014 1857
Size: 157 5689
EcoO109I rG'GnC_Cy
Cuts at: 119 173 1002 1020 1280 2985 119
Size: 54 829 18 260 1705 2980
Fragments arranged by size:
2980 1705 829 260 54 18
EcoRI G'AATT_C
Cuts at: 2850 2850
Size: 5846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 636 2487 3865 636
Size: 1851 1378 2617
Fragments arranged by size:
2617 1851 1378
FspI TGC'GCA
Cuts at: 3627 3627
Size: 5846
HaeII r_GCGC'y
Cuts at: 2527 2611 3280 3528 4550 5529 2527
Size: 84 669 248 1022 979 2844
Fragments arranged by size:
2844 1022 979 669 248 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 867 899 2312 2344 2380 2412 3688 3718 3720
Size: 32 1413 32 36 32 1276 30 2
Cuts at: 3720 3750 867
Size: 30 2963
Fragments arranged by size:
2963 1413 1276 36 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 465 2885 465
Size: 2420 3426
KasI G'GCGC_C
Cuts at: 3276 3524 3276
Size: 248 5598
KpnI G_GTAC'C
Cuts at: 687 2866 687
Size: 2179 3667
MluI A'CGCG_T
Cuts at: 5279 5279
Size: 5846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 743 1148 1430 2522 4517 4701 743
Size: 405 282 1092 1995 184 1888
Fragments arranged by size:
1995 1888 1092 405 282 184
MscI TGG'CCA
Cuts at: 1597 2563 3607 1597
Size: 966 1044 3836
Fragments arranged by size:
3836 1044 966
MslI CAynn'nnrTG
Cuts at: 1360 1871 2512 2641 2707 2736 3835 3962 1360
Size: 511 641 129 66 29 1099 127 3244
Fragments arranged by size:
3244 1099 641 511 129 127 66 29
MspA1I CmG'CkG
Cuts at: 776 843 1275 1662 1826 2768 2957 3271 3631
Size: 67 432 387 164 942 189 314 360
Cuts at: 3631 4644 4889 776
Size: 1013 245 1733
Fragments arranged by size:
1733 1013 942 432 387 360 314 245
189 164 67
NaeI GCC'GGC
Cuts at: 484 4027 484
Size: 3543 2303
NarI GG'CG_CC
Cuts at: 3277 3525 3277
Size: 248 5598
NcoI C'CATG_G
Cuts at: 107 107
Size: 5846
NgoMIV G'CCGG_C
Cuts at: 482 4025 482
Size: 3543 2303
NspI r_CATG'y
Cuts at: 2899 3399 4306 2899
Size: 500 907 4439
Fragments arranged by size:
4439 907 500
PasI CC'CwG_GG
Cuts at: 387 387
Size: 5846
PciI A'CATG_T
Cuts at: 4302 4302
Size: 5846
PfoI T'CCnGG_A
Cuts at: 2378 2378
Size: 5846
PmeI GTTT'AAAC
Cuts at: 2845 2845
Size: 5846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1915 1947 2530 2562 4985 5017 1915
Size: 32 583 32 2423 32 2744
Fragments arranged by size:
2744 2423 583 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1002 1280 1002
Size: 278 5568
PspOMI G'GGCC_C
Cuts at: 1020 1020
Size: 5846
PspXI vC'TCGA_Gb
Cuts at: 1840 1840
Size: 5846
PstI C_TGCA'G
Cuts at: 692 1667 2142 2893 3578 692
Size: 975 475 751 685 2960
Fragments arranged by size:
2960 975 751 685 475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5846
PvuII CAG'CTG
Cuts at: 776 843 1275 1662 2768 3271 3631 776
Size: 67 432 387 1106 503 360 2991
Fragments arranged by size:
2991 1106 503 432 387 360 67
RsrII CG'GwC_CG
Cuts at: 4041 4041
Size: 5846
SacI G_AGCT'C
Cuts at: 2426 2860 2426
Size: 434 5412
SacII CC_GC'GG
Cuts at: 1827 1827
Size: 5846
SalI G'TCGA_C
Cuts at: 2883 2883
Size: 5846
SapI GCTCTTCn'nnn_
Cuts at: 3869 4079 3869
Size: 210 5636
SbfI CC_TGCA'GG
Cuts at: 2893 2893
Size: 5846
ScaI AGT'ACT
Cuts at: 1918 5363 1918
Size: 3445 2401
SfcI C'TryA_G
Cuts at: 33 477 688 1315 1663 2138 2889 3574 4567
Size: 444 211 627 348 475 751 685 993
Cuts at: 4567 4758 33
Size: 191 1121
Fragments arranged by size:
1121 993 751 685 627 475 444 348
211 191
SfoI GGC'GCC
Cuts at: 3278 3526 3278
Size: 248 5598
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
SmaI CCC'GGG
Cuts at: 807 2868 807
Size: 2061 3785
SmlI C'TyrA_G
Cuts at: 1840 1912 2440 2788 3001 3916 4408 4670 1840
Size: 72 528 348 213 915 492 262 3016
Fragments arranged by size:
3016 915 528 492 348 262 213 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5846
SphI G_CATG'C
Cuts at: 2899 3399 2899
Size: 500 5346
StyI C'CwwG_G
Cuts at: 107 2980 3334 107
Size: 2873 354 2619
Fragments arranged by size:
2873 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 499 768 1003 2002 3429 3562 4021 4230 499
Size: 269 235 999 1427 133 459 209 2115
Fragments arranged by size:
2115 1427 999 459 269 235 209 133
TatI w'GTAC_w
Cuts at: 248 770 960 1436 1916 2711 5361 248
Size: 522 190 476 480 795 2650 733
Fragments arranged by size:
2650 795 733 522 480 476 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 252 284 1215 1247 1397 1429 1444 1476 1567
Size: 32 931 32 150 32 15 32 91
Cuts at: 1567 1599 1621 1653 2343 2375 5363 5395 252
Size: 32 22 32 690 32 2988 32 703
Fragments arranged by size:
2988 931 703 690 150 91 32 32
32 32 32 32 32 32 22 15
Tth111I GACn'n_nGTC
Cuts at: 3643 3643
Size: 5846
XbaI T'CTAG_A
Cuts at: 2877 2877
Size: 5846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3341 3341
Size: 5846
XhoI C'TCGA_G
Cuts at: 1840 1840
Size: 5846
XmaI C'CCGG_G
Cuts at: 805 2866 805
Size: 2061 3785
XmnI GAAnn'nnTTC
Cuts at: 1852 1852
Size: 5846
ZraI GAC'GTC
Cuts at: 2067 2067
Size: 5846
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstXI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI