Restriction map for
pF1KA0605
(Circular) (Six-base) MAPSORT of: pF1KA0605.seq Check: 6348 from: 1 to: 5966
pF1KA0605 5966 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1852 2026 2542 1852
Size: 174 516 5276
Fragments arranged by size:
5276 516 174
AatII G_ACGT'C
Cuts at: 2741 2741
Size: 5966
AccI GT'mk_AC
Cuts at: 3004 3004
Size: 5966
Acc65I G'GTAC_C
Cuts at: 395 2982 395
Size: 2587 3379
AclI AA'CG_TT
Cuts at: 1706 5806 1706
Size: 4100 1866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3791 4223 4576 5238 3791
Size: 432 353 662 4519
Fragments arranged by size:
4519 662 432 353
AfeI AGC'GCT
Cuts at: 1155 1155
Size: 5966
AflIII A'CryG_T
Cuts at: 476 1799 2390 2650 3318 5118 5399 476
Size: 1323 591 260 668 1800 281 1043
Fragments arranged by size:
1800 1323 1043 668 591 281 260
AgeI A'CCGG_T
Cuts at: 1805 1805
Size: 5966
AhdI GACnn_n'nnGTC
Cuts at: 180 180
Size: 5966
AleI CACnn'nnGTG
Cuts at: 751 2471 751
Size: 1720 4246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1735 1735
Size: 5966
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1992 2024 1992
Size: 32 5934
AlwNI CAG_nnn'CTG
Cuts at: 374 1035 1170 1494 1507 1916 2690 3271 4709
Size: 661 135 324 13 409 774 581 1438
Cuts at: 4709 374
Size: 1631
Fragments arranged by size:
1631 1438 774 661 581 409 324 135
13
ApaI G_GGCC'C
Cuts at: 361 1022 1297 1361 2168 2311 361
Size: 661 275 64 807 143 4016
Fragments arranged by size:
4016 807 661 275 143 64
ApaLI G'TGCA_C
Cuts at: 2196 2700 4804 2196
Size: 504 2104 3358
Fragments arranged by size:
3358 2104 504
ApoI r'AATT_y
Cuts at: 2970 5319 5575 2970
Size: 2349 256 3361
Fragments arranged by size:
3361 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5966
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
AvaI C'yCGr_G
Cuts at: 354 361 824 1272 1936 2128 2611 2638 2986
Size: 7 463 448 664 192 483 27 348
Cuts at: 2986 354
Size: 3334
Fragments arranged by size:
3334 664 483 463 448 348 192 27
7
BamHI G'GATC_C
Cuts at: 2991 2991
Size: 5966
BanI G'GyrC_C
Cuts at: 232 395 820 1561 2869 2982 3396 3644 3679
Size: 163 425 741 1308 113 414 248 35
Cuts at: 3679 232
Size: 2519
Fragments arranged by size:
2519 1308 741 425 414 248 163 113
35
BanII G_rGCy'C
Cuts at: 361 668 1022 1297 1361 1576 1832 2168 2311
Size: 307 354 275 64 215 256 336 143
Cuts at: 2311 2624 2840 2980 4010 361
Size: 313 216 140 1030 2317
Fragments arranged by size:
2317 1030 354 336 313 307 275 256
216 215 143 140 64
BbeI G_GCGC'C
Cuts at: 1565 3400 3648 1565
Size: 1835 248 3883
Fragments arranged by size:
3883 1835 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 626 660 1654 1688 2182 2216 2637 2671 626
Size: 34 994 34 494 34 421 34 3921
Fragments arranged by size:
3921 994 494 421 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3864 4920 5966 3864
Size: 1056 1046 3864
Fragments arranged by size:
3864 1056 1046
BclI T'GATC_A
Cuts at: 3486 3486
Size: 5966
BglI GCCn_nnn'nGGC
Cuts at: 1274 1274
Size: 5966
BglII A'GATC_T
Cuts at: 3481 5518 3481
Size: 2037 3929
BlpI GC'TnA_GC
Cuts at: 1657 3078 1657
Size: 1421 4545
Bme1580I G_kGCm'C
Cuts at: 235 361 385 825 1022 1297 1361 2168 2200
Size: 126 24 440 197 275 64 807 32
Cuts at: 2200 2311 2704 2883 2912 3591 3684 4808 235
Size: 111 393 179 29 679 93 1124 1393
Fragments arranged by size:
1393 1124 807 679 440 393 275 197
179 126 111 93 64 32 29 24
BmgBI CAC'GTC
Cuts at: 386 864 386
Size: 478 5488
BmrI ACTGGGnnnn_n'
Cuts at: 2163 2352 2427 2523 3178 3259 3594 5738 2163
Size: 189 75 96 655 81 335 2144 2391
Fragments arranged by size:
2391 2144 655 335 189 96 81 75
BmtI G_CTAG'C
Cuts at: 871 871
Size: 5966
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1482 4333 1482
Size: 2851 3115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 522 1672 2019 244
Size: 278 1150 347 4191
Fragments arranged by size:
4191 1150 347 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3142 4021 4735 5033 3142
Size: 879 714 298 4075
Fragments arranged by size:
4075 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 982 1306 1376 2440 5670 982
Size: 324 70 1064 3230 1278
Fragments arranged by size:
3230 1278 1064 324 70
BsaAI yAC'GTr
Cuts at: 477 753 2391 2651 3319 477
Size: 276 1638 260 668 3124
Fragments arranged by size:
3124 1638 668 276 260
BsaBI GATnn'nnATC
Cuts at: 6 3480 5517 6
Size: 3474 2037 455
Fragments arranged by size:
3474 2037 455
BsaHI Gr'CG_yC
Cuts at: 239 1562 1895 1922 2738 3397 3645 4347 5843
Size: 1323 333 27 816 659 248 702 1496
Cuts at: 5843 239
Size: 362
Fragments arranged by size:
1496 1323 816 702 659 362 333 248
27
BsaWI w'CCGG_w
Cuts at: 991 1805 3155 3676 4354 4765 4912 5186 991
Size: 814 1350 521 678 411 147 274 1771
Fragments arranged by size:
1771 1350 814 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1195 1225 1386 1416 1435 1465 1669 1699 1992
Size: 30 161 30 19 30 204 30 293
Cuts at: 1992 2022 2635 2665 1195
Size: 30 613 30 4496
Fragments arranged by size:
4496 613 293 204 161 30 30 30
30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 354 450 463 1221 1394 1621 1674 2656 354
Size: 96 13 758 173 227 53 982 3664
Fragments arranged by size:
3664 982 758 227 173 96 53 13
BseYI C'CCAG_C
Cuts at: 134 169 1124 1242 1290 1297 1494 1960 3391
Size: 35 955 118 48 7 197 466 1431
Cuts at: 3391 4149 4814 5222 134
Size: 758 665 408 878
Fragments arranged by size:
1431 955 878 758 665 466 408 197
118 48 35 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 433 541 729 1830 2286 2373 360
Size: 73 108 188 1101 456 87 3953
Fragments arranged by size:
3953 1101 456 188 108 87 73
BsiEI CG_ry'CG
Cuts at: 104 1733 3508 3554 4784 104
Size: 1629 1775 46 1230 1286
Fragments arranged by size:
1775 1629 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1576 2200 2567 2650 2704 2980 3758 3948 4808
Size: 624 367 83 54 276 778 190 860
Cuts at: 4808 1576
Size: 2734
Fragments arranged by size:
2734 860 778 624 367 276 190 83
54
BsiWI C'GTAC_G
Cuts at: 1868 1868
Size: 5966
BsmI GAATG_Cn'
Cuts at: 783 783
Size: 5966
BsmBI CGTCTCn'nnnn_
Cuts at: 465 1981 2340 2729 465
Size: 1516 359 389 3702
Fragments arranged by size:
3702 1516 389 359
Bsp1286I G_dGCh'C
Cuts at: 235 361 385 668 825 1022 1297 1361 1576
Size: 126 24 283 157 197 275 64 215
Cuts at: 1576 1832 2168 2200 2311 2567 2624 2650 2704
Size: 256 336 32 111 256 57 26 54
Cuts at: 2704 2840 2883 2912 2980 3591 3684 3758 3948
Size: 136 43 29 68 611 93 74 190
Cuts at: 3948 4010 4808 235
Size: 62 798 1393
Fragments arranged by size:
1393 798 611 336 283 275 256 256
215 197 190 157 136 126 111 93
74 68 64 62 57 54 43 32
29 26 24
BspHI T'CATG_A
Cuts at: 4398 4398
Size: 5966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 563 641 1640 1852 2026 2152 2542 2719
Size: 181 78 999 212 174 126 390 177
Cuts at: 2719 3016 3230 3532 3913 382
Size: 297 214 302 381 2435
Fragments arranged by size:
2435 999 390 381 302 297 214 212
181 177 174 126 78
BsrBI CCG'CTC
Cuts at: 50 321 1239 2520 2939 4258 4312 5796 5961
Size: 271 918 1281 419 1319 54 1484 165
Cuts at: 5961 50
Size: 55
Fragments arranged by size:
1484 1319 1281 918 419 271 165 55
54
BsrDI GCAATG_nn'
Cuts at: 130 3878 130
Size: 3748 2218
BsrFI r'CCGG_y
Cuts at: 965 1256 1336 1805 2321 3964 4145 965
Size: 291 80 469 516 1643 181 2786
Fragments arranged by size:
2786 1643 516 469 291 181 80
BsrGI T'GTAC_A
Cuts at: 479 2090 479
Size: 1611 4355
BssHII G'CGCG_C
Cuts at: 1771 1771
Size: 5966
BssSI C'ACGA_G
Cuts at: 1111 1825 1948 4237 4945 1111
Size: 714 123 2289 708 2132
Fragments arranged by size:
2289 2132 714 708 123
BstAPI GCAn_nnn'nTGC
Cuts at: 330 617 2144 2930 3343 330
Size: 287 1527 786 413 2953
Fragments arranged by size:
2953 1527 786 413 287
BstBI TT'CG_AA
Cuts at: 4327 4327
Size: 5966
BstEII G'GTnAC_C
Cuts at: 801 2226 801
Size: 1425 4541
BstXI CCAn_nnnn'nTGG
Cuts at: 222 2457 2473 222
Size: 2235 16 3715
Fragments arranged by size:
3715 2235 16
BstYI r'GATC_y
Cuts at: 1 2991 3458 3481 3816 4062 4368 4380 4466
Size: 2990 467 23 335 246 306 12 86
Cuts at: 4466 5518 1
Size: 1052 449
Fragments arranged by size:
2990 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 194 242 569 974 1793 2769 2831 107
Size: 87 48 327 405 819 976 62 3242
Fragments arranged by size:
3242 976 819 405 327 87 62 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 927 3916 4097 4260 927
Size: 2989 181 163 2633
Fragments arranged by size:
2989 2633 181 163
BtsI GCAGTG_nn'
Cuts at: 324 460 1842 2198 2226 2332 2361 2610 3209
Size: 136 1382 356 28 106 29 249 599
Cuts at: 3209 324
Size: 3081
Fragments arranged by size:
3081 1382 599 356 249 136 106 29
28
DraI TTT'AAA
Cuts at: 2965 2965
Size: 5966
DrdI GACnn_nn'nnGTC
Cuts at: 1390 3672 5016 1390
Size: 2282 1344 2340
Fragments arranged by size:
2340 2282 1344
EaeI y'GGCC_r
Cuts at: 576 1221 1266 1580 1730 2459 2579 2885 3551
Size: 645 45 314 150 729 120 306 666
Cuts at: 3551 3725 4116 4143 5488 576
Size: 174 391 27 1345 1054
Fragments arranged by size:
1345 1054 729 666 645 391 314 306
174 150 120 45 27
EagI C'GGCC_G
Cuts at: 1730 3551 1730
Size: 1821 4145
EarI CTCTTCn'nnn_
Cuts at: 1424 3989 4199 1424
Size: 2565 210 3191
Fragments arranged by size:
3191 2565 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 413 504 4174 4916 5062 5776 413
Size: 91 3670 742 146 714 603
Fragments arranged by size:
3670 742 714 603 146 91
EcoICRI GAG'CTC
Cuts at: 1574 2978 1574
Size: 1404 4562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 522 1672 2019 3791 4223 4576 5238 244
Size: 278 1150 347 1772 432 353 662 972
Fragments arranged by size:
1772 1150 972 662 432 353 347 278
EcoNI CCTnn'n_nnAGG
Cuts at: 2104 2104
Size: 5966
EcoO109I rG'GnC_Cy
Cuts at: 358 371 1018 1032 1167 1294 1346 1357 1358
Size: 13 647 14 135 127 52 11 1
Cuts at: 1358 1643 1692 2165 2663 2948 3105 358
Size: 285 49 473 498 285 157 3219
Fragments arranged by size:
3219 647 498 473 285 285 157 135
127 52 49 14 13 11 1
EcoRI G'AATT_C
Cuts at: 2970 2970
Size: 5966
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3985 3985
Size: 5966
FspI TGC'GCA
Cuts at: 3747 3747
Size: 5966
HaeII r_GCGC'y
Cuts at: 1157 1565 3400 3648 4878 5649 1157
Size: 408 1835 248 1230 771 1474
Fragments arranged by size:
1835 1474 1230 771 408 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 598 630 2439 2471 3808 3838 3840
Size: 32 400 32 1809 32 1337 30 2
Cuts at: 3840 3870 166
Size: 30 2262
Fragments arranged by size:
2262 1809 1337 400 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 185 1437 3005 185
Size: 1252 1568 3146
Fragments arranged by size:
3146 1568 1252
KasI G'GCGC_C
Cuts at: 1561 3396 3644 1561
Size: 1835 248 3883
Fragments arranged by size:
3883 1835 248
KpnI G_GTAC'C
Cuts at: 399 2986 399
Size: 2587 3379
MluI A'CGCG_T
Cuts at: 5399 5399
Size: 5966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2162 2363 4725 4909 2162
Size: 201 2362 184 3219
Fragments arranged by size:
3219 2362 201 184
MscI TGG'CCA
Cuts at: 2461 2581 2887 3727 2461
Size: 120 306 840 4700
Fragments arranged by size:
4700 840 306 120
MslI CAynn'nnrTG
Cuts at: 751 2471 2893 3955 4082 751
Size: 1720 422 1062 127 2635
Fragments arranged by size:
2635 1720 1062 422 127
MspA1I CmG'CkG
Cuts at: 582 743 859 1491 1795 1997 2243 2852 3077
Size: 161 116 632 304 202 246 609 225
Cuts at: 3077 3391 3751 4535 4780 582
Size: 314 360 784 245 1768
Fragments arranged by size:
1768 784 632 609 360 314 304 246
245 225 202 161 116
NaeI GCC'GGC
Cuts at: 1338 4147 1338
Size: 2809 3157
NarI GG'CG_CC
Cuts at: 1562 3397 3645 1562
Size: 1835 248 3883
Fragments arranged by size:
3883 1835 248
NcoI C'CATG_G
Cuts at: 107 974 107
Size: 867 5099
NgoMIV G'CCGG_C
Cuts at: 1336 4145 1336
Size: 2809 3157
NheI G'CTAG_C
Cuts at: 867 867
Size: 5966
NspI r_CATG'y
Cuts at: 1803 3019 3519 5122 1803
Size: 1216 500 1603 2647
Fragments arranged by size:
2647 1603 1216 500
PasI CC'CwG_GG
Cuts at: 1364 1364
Size: 5966
PciI A'CATG_T
Cuts at: 1799 5118 1799
Size: 3319 2647
PflMI CCAn_nnn'nTGG
Cuts at: 705 1170 1494 2502 2690 705
Size: 465 324 1008 188 3981
Fragments arranged by size:
3981 1008 465 324 188
PmeI GTTT'AAAC
Cuts at: 2965 2965
Size: 5966
PmlI CAC'GTG
Cuts at: 477 753 2391 2651 477
Size: 276 1638 260 3792
Fragments arranged by size:
3792 1638 276 260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1992 2024 4412 4444 1992
Size: 32 2388 32 3514
Fragments arranged by size:
3514 2388 32 32
PpuMI rG'GwC_Cy
Cuts at: 371 1643 371
Size: 1272 4694
PspOMI G'GGCC_C
Cuts at: 357 1018 1293 1357 2164 2307 357
Size: 661 275 64 807 143 4016
Fragments arranged by size:
4016 807 661 275 143 64
PstI C_TGCA'G
Cuts at: 335 409 1103 1837 2822 2947 3013 3698 335
Size: 74 694 734 985 125 66 685 2603
Fragments arranged by size:
2603 985 734 694 685 125 74 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5966
PvuII CAG'CTG
Cuts at: 743 1491 1997 2852 3391 3751 743
Size: 748 506 855 539 360 2958
Fragments arranged by size:
2958 855 748 539 506 360
RsrII CG'GwC_CG
Cuts at: 4161 4161
Size: 5966
SacI G_AGCT'C
Cuts at: 1576 2980 1576
Size: 1404 4562
SacII CC_GC'GG
Cuts at: 1796 1796
Size: 5966
SalI G'TCGA_C
Cuts at: 3003 3003
Size: 5966
SapI GCTCTTCn'nnn_
Cuts at: 3989 4199 3989
Size: 210 5756
SbfI CC_TGCA'GG
Cuts at: 2947 3013 2947
Size: 66 5900
ScaI AGT'ACT
Cuts at: 5483 5483
Size: 5966
SfcI C'TryA_G
Cuts at: 33 331 405 1099 1833 2285 2818 2919 2943
Size: 298 74 694 734 452 533 101 24
Cuts at: 2943 3009 3694 4662 4853 33
Size: 66 685 968 191 1146
Fragments arranged by size:
1146 968 734 694 685 533 452 298
191 101 74 66 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 1274 1274
Size: 5966
SfoI GGC'GCC
Cuts at: 1563 3398 3646 1563
Size: 1835 248 3883
Fragments arranged by size:
3883 1835 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5966
SmaI CCC'GGG
Cuts at: 356 363 1274 2988 356
Size: 7 911 1714 3334
Fragments arranged by size:
3334 1714 911 7
SmlI C'TyrA_G
Cuts at: 3121 4036 4750 5012 3121
Size: 915 714 262 4075
Fragments arranged by size:
4075 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5966
SphI G_CATG'C
Cuts at: 3019 3519 3019
Size: 500 5466
StuI AGG'CCT
Cuts at: 1228 2283 2809 1228
Size: 1055 526 4385
Fragments arranged by size:
4385 1055 526
StyI C'CwwG_G
Cuts at: 107 786 974 1567 1766 3100 3454 107
Size: 679 188 593 199 1334 354 2619
Fragments arranged by size:
2619 1334 679 593 354 199 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 712 763 1456 2773 3549 3682 4141 4350 712
Size: 51 693 1317 776 133 459 209 2328
Fragments arranged by size:
2328 1317 776 693 459 209 133 51
TatI w'GTAC_w
Cuts at: 479 1001 1088 2090 2393 5481 479
Size: 522 87 1002 303 3088 964
Fragments arranged by size:
3088 1002 964 522 303 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1195 1227 1386 1418 1435 1467 2635 2667 5483
Size: 32 159 32 17 32 1168 32 2816
Cuts at: 5483 5515 1195
Size: 32 1646
Fragments arranged by size:
2816 1646 1168 159 32 32 32 32
32 17
Tth111I GACn'n_nGTC
Cuts at: 526 2764 3763 526
Size: 2238 999 2729
Fragments arranged by size:
2729 2238 999
XbaI T'CTAG_A
Cuts at: 2997 2997
Size: 5966
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 702 814 2880 3461 702
Size: 112 2066 581 3207
Fragments arranged by size:
3207 2066 581 112
XmaI C'CCGG_G
Cuts at: 354 361 1272 2986 354
Size: 7 911 1714 3334
Fragments arranged by size:
3334 1714 911 7
XmnI GAAnn'nnTTC
Cuts at: 1519 1519
Size: 5966
ZraI GAC'GTC
Cuts at: 2739 2739
Size: 5966
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BbsI BbvCI BfrBI Bpu10I
BspEI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PfoI PshAI PsiI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI