Restriction map for
pF1KA0598
(Circular) (Six-base) MAPSORT of: pF1KA0598.seq Check: 3232 from: 1 to: 4796
pF1KA0598 4796 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1424 1834 1424
Size: 410 4386
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 2621 3053 3800 4068 314
Size: 2307 432 747 268 1042
Fragments arranged by size:
2307 1042 747 432 268
AfeI AGC'GCT
Cuts at: 778 988 778
Size: 210 4586
AflII C'TTAA_G
Cuts at: 634 1608 634
Size: 974 3822
AflIII A'CryG_T
Cuts at: 2148 3252 4229 2148
Size: 1104 977 2715
Fragments arranged by size:
2715 1104 977
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AhdI GACnn_n'nnGTC
Cuts at: 1270 1270
Size: 4796
AleI CACnn'nnGTG
Cuts at: 972 972
Size: 4796
AlwNI CAG_nnn'CTG
Cuts at: 1593 2101 3668 1593
Size: 508 1567 2721
Fragments arranged by size:
2721 1567 508
ApaI G_GGCC'C
Cuts at: 187 411 1607 1688 187
Size: 224 1196 81 3295
Fragments arranged by size:
3295 1196 224 81
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 233 676 1800 4149 4405 233
Size: 443 1124 2349 256 624
Fragments arranged by size:
2349 1124 624 443 256
AseI AT'TA_AT
Cuts at: 20 122 568 20
Size: 102 446 4248
Fragments arranged by size:
4248 446 102
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 720 1666 1816 720
Size: 946 150 3700
Fragments arranged by size:
3700 946 150
AvrII C'CTAG_G
Cuts at: 1599 1599
Size: 4796
BamHI G'GATC_C
Cuts at: 1821 1821
Size: 4796
BanI G'GyrC_C
Cuts at: 1812 2226 2474 2509 1812
Size: 414 248 35 4099
Fragments arranged by size:
4099 414 248 35
BanII G_rGCy'C
Cuts at: 187 411 700 1475 1607 1645 1688 1810 2840
Size: 224 289 775 132 38 43 122 1030
Cuts at: 2840 187
Size: 2143
Fragments arranged by size:
2143 1030 775 289 224 132 122 43
38
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 635 1575 1789 635
Size: 940 214 3642
Fragments arranged by size:
3642 940 214
BciVI GTATCCnnnnn_n'
Cuts at: 2694 3455 4796 2694
Size: 761 1341 2694
Fragments arranged by size:
2694 1341 761
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BfrBI ATG'CAT
Cuts at: 1552 1552
Size: 4796
BglI GCCn_nnn'nGGC
Cuts at: 182 1458 1692 182
Size: 1276 234 3286
Fragments arranged by size:
3286 1276 234
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 1501 1908 1501
Size: 407 4389
Bme1580I G_kGCm'C
Cuts at: 187 211 411 1607 1688 2421 2514 3570 187
Size: 24 200 1196 81 733 93 1056 1413
Fragments arranged by size:
1413 1196 1056 733 200 93 81 24
BmrI ACTGGGnnnn_n'
Cuts at: 318 2008 2089 2424 4568 318
Size: 1690 81 335 2144 546
Fragments arranged by size:
2144 1690 546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 735 3163 735
Size: 2428 2368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 1447
Size: 4796
Bpu10I CC'TnA_GC
Cuts at: 1279 1279
Size: 4796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1972 2851 3343 3641 1972
Size: 879 492 298 3127
Fragments arranged by size:
3127 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1016 4500 1016
Size: 3484 1312
BsaAI yAC'GTr
Cuts at: 215 762 2149 215
Size: 547 1387 2862
Fragments arranged by size:
2862 1387 547
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 797 1662 2227 2475 3177 4673 797
Size: 865 565 248 702 1496 920
Fragments arranged by size:
1496 920 865 702 565 248
BsaWI w'CCGG_w
Cuts at: 1673 1985 2506 3184 3458 3605 4016 1673
Size: 312 521 678 274 147 411 2453
Fragments arranged by size:
2453 678 521 411 312 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1285 1315 1285
Size: 30 4766
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 727 727
Size: 4796
BseYI C'CCAG_C
Cuts at: 457 466 2221 2979 3556 4052 457
Size: 9 1755 758 577 496 1201
Fragments arranged by size:
1755 1201 758 577 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 916
Size: 4796
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 767 1810 2588 2778 3570 767
Size: 1043 778 190 792 1993
Fragments arranged by size:
1993 1043 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 187 211 411 700 767 1475 1607 1645 1688
Size: 24 200 289 67 708 132 38 43
Cuts at: 1688 1810 2421 2514 2588 2778 2840 3570 187
Size: 122 611 93 74 190 62 730 1413
Fragments arranged by size:
1413 730 708 611 289 200 190 132
122 93 74 67 62 43 38 24
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 1743 1846 2060 2362 2743 156
Size: 1587 103 214 302 381 2209
Fragments arranged by size:
2209 1587 381 302 214 103
BsrBI CCG'CTC
Cuts at: 50 785 3088 3142 4626 4791 50
Size: 735 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 735 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2708 2708
Size: 4796
BsrFI r'CCGG_y
Cuts at: 2794 2975 3184 2794
Size: 181 209 4406
Fragments arranged by size:
4406 209 181
BssHII G'CGCG_C
Cuts at: 1413 1413
Size: 4796
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 125 648 1654 2173 125
Size: 523 1006 519 2748
Fragments arranged by size:
2748 1006 523 519
BstBI TT'CG_AA
Cuts at: 1330 3157 1330
Size: 1827 2969
BstEII G'GTnAC_C
Cuts at: 196 196
Size: 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 474 974 474
Size: 500 4296
BstYI r'GATC_y
Cuts at: 1 1076 1821 2288 2311 2646 2892 3904 3990
Size: 1075 745 467 23 335 246 1012 86
Cuts at: 3990 4002 4348 1
Size: 12 346 449
Fragments arranged by size:
1075 1012 745 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 938 938
Size: 4796
BtgI C'CryG_G
Cuts at: 1034 1340 1034
Size: 306 4490
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 357 2746 2927 3090 357
Size: 2389 181 163 2063
Fragments arranged by size:
2389 2063 181 163
BtsI GCAGTG_nn'
Cuts at: 114 1372 1408 2039 114
Size: 1258 36 631 2871
Fragments arranged by size:
2871 1258 631 36
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DraIII CAC_nnn'GTG
Cuts at: 1163 1163
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 1064 2381 2555 2946 2973 4318 1064
Size: 1317 174 391 27 1345 1542
Fragments arranged by size:
1542 1345 1317 391 174 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 841 942 991 3004 3314 3460 4606 841
Size: 101 49 2013 310 146 1146 1031
Fragments arranged by size:
2013 1146 1031 310 146 101 49
EcoICRI GAG'CTC
Cuts at: 1808 1808
Size: 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 1447 2621 3053 3800 4068 314
Size: 1133 1174 432 747 268 1042
Fragments arranged by size:
1174 1133 1042 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 805 805
Size: 4796
EcoO109I rG'GnC_Cy
Cuts at: 183 184 407 1284 1603 1604 1684 1730 1755
Size: 1 223 877 319 1 80 46 25
Cuts at: 1755 1935 183
Size: 180 3044
Fragments arranged by size:
3044 877 319 223 180 80 46 25
1 1
EcoRI G'AATT_C
Cuts at: 1800 1800
Size: 4796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 805 2577 805
Size: 1772 3024
HaeII r_GCGC'y
Cuts at: 780 990 2230 2478 3500 4479 780
Size: 210 1240 248 1022 979 1097
Fragments arranged by size:
1240 1097 1022 979 248 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 335 367 1256 1288 1537 1569 2638 2668 2670
Size: 32 889 32 249 32 1069 30 2
Cuts at: 2670 2700 335
Size: 30 2431
Fragments arranged by size:
2431 1069 889 249 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 171 1835 171
Size: 1664 3132
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1580 3467 3651 1580
Size: 1887 184 2725
Fragments arranged by size:
2725 1887 184
MscI TGG'CCA
Cuts at: 2557 2557
Size: 4796
MslI CAynn'nnrTG
Cuts at: 972 1453 2785 2912 972
Size: 481 1332 127 2856
Fragments arranged by size:
2856 1332 481 127
MspA1I CmG'CkG
Cuts at: 138 1342 1380 1907 2221 2581 3594 3839 138
Size: 1204 38 527 314 360 1013 245 1095
Fragments arranged by size:
1204 1095 1013 527 360 314 245 38
NaeI GCC'GGC
Cuts at: 2977 2977
Size: 4796
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NdeI CA'TA_TG
Cuts at: 653 653
Size: 4796
NgoMIV G'CCGG_C
Cuts at: 2975 2975
Size: 4796
NsiI A_TGCA'T
Cuts at: 1554 1554
Size: 4796
NspI r_CATG'y
Cuts at: 1398 1849 2349 3256 1398
Size: 451 500 907 2938
Fragments arranged by size:
2938 907 500 451
PciI A'CATG_T
Cuts at: 3252 3252
Size: 4796
PflMI CCAn_nnn'nTGG
Cuts at: 1163 1163
Size: 4796
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PmlI CAC'GTG
Cuts at: 215 215
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 931 963 3935 3967 931
Size: 32 2972 32 1760
Fragments arranged by size:
2972 1760 32 32
PpuMI rG'GwC_Cy
Cuts at: 1284 1755 1284
Size: 471 4325
PspOMI G'GGCC_C
Cuts at: 183 407 1603 1684 183
Size: 224 1196 81 3295
Fragments arranged by size:
3295 1196 224 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 700 732 700
Size: 32 4764
PstI C_TGCA'G
Cuts at: 1094 1379 1843 2528 1094
Size: 285 464 685 3362
Fragments arranged by size:
3362 685 464 285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 138 1380 2221 2581 138
Size: 1242 841 360 2353
Fragments arranged by size:
2353 1242 841 360
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1810 1810
Size: 4796
SacII CC_GC'GG
Cuts at: 1343 1343
Size: 4796
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SanDI GG'GwC_CC
Cuts at: 1284 1284
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 1090 1375 1725 1839 2524 3517 3708 33
Size: 1057 285 350 114 685 993 191 1121
Fragments arranged by size:
1121 1057 993 685 350 285 191 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 1692 1692
Size: 4796
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1818 1818
Size: 4796
SmlI C'TyrA_G
Cuts at: 634 1608 1951 2866 3358 3620 634
Size: 974 343 915 492 262 1810
Fragments arranged by size:
1810 974 915 492 343 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1398 1849 2349 1398
Size: 451 500 3845
Fragments arranged by size:
3845 500 451
SspI AAT'ATT
Cuts at: 534 534
Size: 4796
StuI AGG'CCT
Cuts at: 811 811
Size: 4796
StyI C'CwwG_G
Cuts at: 177 377 1599 1930 2284 177
Size: 200 1222 331 354 2689
Fragments arranged by size:
2689 1222 354 331 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2379 2512 2971 3180 2379
Size: 133 459 209 3995
Fragments arranged by size:
3995 459 209 133
TatI w'GTAC_w
Cuts at: 1301 1565 4311 1301
Size: 264 2746 1786
Fragments arranged by size:
2746 1786 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 502 534 1061 1093 4313 4345 502
Size: 32 527 32 3220 32 953
Fragments arranged by size:
3220 953 527 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2593 2593
Size: 4796
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2291
Size: 4796
XmaI C'CCGG_G
Cuts at: 1816 1816
Size: 4796
XmnI GAAnn'nnTTC
Cuts at: 847 847
Size: 4796
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NsiI NspI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AlfI AloI AscI BaeI BbvCI BcgI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NruI PacI PasI PfoI PshAI PsiI
PspXI SexAI SgrAI SnaBI SrfI SwaI XhoI ZraI