Restriction map for
pF1KA0595
(Circular) (Six-base) MAPSORT of: pF1KA0595.seq Check: 5925 from: 1 to: 8105
pF1KA0595 8105 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 249 1452 2634 2746 249
Size: 1203 1182 112 5608
Fragments arranged by size:
5608 1203 1182 112
AccI GT'mk_AC
Cuts at: 1567 1598 1838 3091 5143 1567
Size: 31 240 1253 2052 4529
Fragments arranged by size:
4529 2052 1253 240 31
Acc65I G'GTAC_C
Cuts at: 2320 5121 2320
Size: 2801 5304
AclI AA'CG_TT
Cuts at: 7945 7945
Size: 8105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 855 1081 2300 3389 3590 3916 3923 5930 6362
Size: 226 1219 1089 201 326 7 2007 432
Cuts at: 6362 6715 7377 855
Size: 353 662 1583
Fragments arranged by size:
2007 1583 1219 1089 662 432 353 326
226 201 7
AflIII A'CryG_T
Cuts at: 3034 5457 7257 7538 3034
Size: 2423 1800 281 3601
Fragments arranged by size:
3601 2423 1800 281
AgeI A'CCGG_T
Cuts at: 3752 3752
Size: 8105
AleI CACnn'nnGTG
Cuts at: 4835 4835
Size: 8105
AlwNI CAG_nnn'CTG
Cuts at: 264 526 997 1306 3289 5029 5410 6848 264
Size: 262 471 309 1983 1740 381 1438 1521
Fragments arranged by size:
1983 1740 1521 1438 471 381 309 262
ApaI G_GGCC'C
Cuts at: 163 319 671 3050 3064 4123 163
Size: 156 352 2379 14 1059 4145
Fragments arranged by size:
4145 2379 1059 352 156 14
ApaLI G'TGCA_C
Cuts at: 4821 6943 4821
Size: 2122 5983
ApoI r'AATT_y
Cuts at: 1707 5041 5109 7458 7714 1707
Size: 3334 68 2349 256 2098
Fragments arranged by size:
3334 2349 2098 256 68
AscI GG'CGCG_CC
Cuts at: 115 115
Size: 8105
AseI AT'TA_AT
Cuts at: 20 20
Size: 8105
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8105
AvaI C'yCGr_G
Cuts at: 150 581 1670 1682 4157 5125 150
Size: 431 1089 12 2475 968 3130
Fragments arranged by size:
3130 2475 1089 968 431 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1568 1601 1568
Size: 33 8072
BamHI G'GATC_C
Cuts at: 5130 5130
Size: 8105
BanI G'GyrC_C
Cuts at: 1419 2320 2646 2934 2959 2997 5121 5535 5783
Size: 901 326 288 25 38 2124 414 248
Cuts at: 5783 5818 1419
Size: 35 3706
Fragments arranged by size:
3706 2124 901 414 326 288 248 38
35 25
BanII G_rGCy'C
Cuts at: 163 189 319 592 671 1476 1683 3050 3064
Size: 26 130 273 79 805 207 1367 14
Cuts at: 3064 3249 3791 3985 4063 4123 5119 6149 163
Size: 185 542 194 78 60 996 1030 2119
Fragments arranged by size:
2119 1367 1030 996 805 542 273 207
194 185 130 79 78 60 26 14
BbeI G_GCGC'C
Cuts at: 5539 5787 5539
Size: 248 7857
BbsI GAAGACnn'nnnn_
Cuts at: 2524 2803 4442 4571 4734 5018 2524
Size: 279 1639 129 163 284 5611
Fragments arranged by size:
5611 1639 284 279 163 129
BbvCI CC'TCA_GC
Cuts at: 1543 4203 4293 4880 1543
Size: 2660 90 587 4768
Fragments arranged by size:
4768 2660 587 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4313 4347 4313
Size: 34 8071
BciVI GTATCCnnnnn_n'
Cuts at: 928 6003 7059 8105 928
Size: 5075 1056 1046 928
Fragments arranged by size:
5075 1056 1046 928
BclI T'GATC_A
Cuts at: 2000 4170 5625 2000
Size: 2170 1455 4480
Fragments arranged by size:
4480 2170 1455
BfrBI ATG'CAT
Cuts at: 936 4145 936
Size: 3209 4896
BglI GCCn_nnn'nGGC
Cuts at: 112 2874 3781 4376 112
Size: 2762 907 595 3841
Fragments arranged by size:
3841 2762 907 595
BglII A'GATC_T
Cuts at: 506 5620 7657 506
Size: 5114 2037 954
Fragments arranged by size:
5114 2037 954
BlpI GC'TnA_GC
Cuts at: 1988 5217 1988
Size: 3229 4876
Bme1580I G_kGCm'C
Cuts at: 163 319 671 1316 1424 2717 2939 3000 3050
Size: 156 352 645 108 1293 222 61 50
Cuts at: 3050 3064 3169 4123 4825 5730 5823 6947 163
Size: 14 105 954 702 905 93 1124 1321
Fragments arranged by size:
1321 1293 1124 954 905 702 645 352
222 156 108 105 93 61 50 14
BmgBI CAC'GTC
Cuts at: 2191 2191
Size: 8105
BmrI ACTGGGnnnn_n'
Cuts at: 646 665 673 1186 2002 2149 2444 2771 3007
Size: 19 8 513 816 147 295 327 236
Cuts at: 3007 5317 5398 5733 7877 646
Size: 2310 81 335 2144 874
Fragments arranged by size:
2310 2144 874 816 513 335 327 295
236 147 81 19 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 609 1029 4077 6472 609
Size: 420 3048 2395 2242
Fragments arranged by size:
3048 2395 2242 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 801 1165 1204 1309 1636 2129 3101 3155
Size: 317 364 39 105 327 493 972 54
Cuts at: 3155 3655 3782 484
Size: 500 127 4807
Fragments arranged by size:
4807 972 500 493 364 327 317 127
105 54 39
Bpu10I CC'TnA_GC
Cuts at: 1543 2600 4203 4293 4880 1543
Size: 1057 1603 90 587 4768
Fragments arranged by size:
4768 1603 1057 587 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 2572 4335 5281 6160 6874 7172 1105
Size: 1467 1763 946 879 714 298 2038
Fragments arranged by size:
2038 1763 1467 946 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 748 777 1215 2670 3409 3418 7809 748
Size: 29 438 1455 739 9 4391 1044
Fragments arranged by size:
4391 1455 1044 739 438 29 9
BsaAI yAC'GTr
Cuts at: 3556 5458 3556
Size: 1902 6203
BsaBI GATnn'nnATC
Cuts at: 6 4623 5619 7656 6
Size: 4617 996 2037 455
Fragments arranged by size:
4617 2037 996 455
BsaHI Gr'CG_yC
Cuts at: 5536 5784 6486 7982 5536
Size: 248 702 1496 5659
Fragments arranged by size:
5659 1496 702 248
BsaWI w'CCGG_w
Cuts at: 168 3752 4007 4412 5294 5815 6493 6904 7051
Size: 3584 255 405 882 521 678 411 147
Cuts at: 7051 7325 168
Size: 274 948
Fragments arranged by size:
3584 948 882 678 521 411 405 274
255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2216 2233 2246 2263 4263 4293 2216
Size: 17 13 17 2000 30 6028
Fragments arranged by size:
6028 2000 30 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 364 582 868 871 967 1005 1378 2224
Size: 75 218 286 3 96 38 373 846
Cuts at: 2224 3203 3239 3400 3665 4831 4897 4995 289
Size: 979 36 161 265 1166 66 98 3399
Fragments arranged by size:
3399 1166 979 846 373 286 265 218
161 98 96 75 66 38 36 3
BseYI C'CCAG_C
Cuts at: 312 378 806 827 2094 2228 2508 2578 3516
Size: 66 428 21 1267 134 280 70 938
Cuts at: 3516 4122 5022 5530 6288 6953 7361 312
Size: 606 900 508 758 665 408 1056
Fragments arranged by size:
1267 1056 938 900 758 665 606 508
428 408 280 134 70 66 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 2216 2713 583
Size: 1633 497 5975
Fragments arranged by size:
5975 1633 497
BsiEI CG_ry'CG
Cuts at: 104 5647 5693 6923 104
Size: 5543 46 1230 1286
Fragments arranged by size:
5543 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1235 1476 3249 3490 4825 5119 5897 6087 6947
Size: 241 1773 241 1335 294 778 190 860
Cuts at: 6947 1235
Size: 2393
Fragments arranged by size:
2393 1773 1335 860 778 294 241 241
190
BsmI GAATG_Cn'
Cuts at: 551 551
Size: 8105
BsmBI CGTCTCn'nnnn_
Cuts at: 125 125
Size: 8105
Bsp1286I G_dGCh'C
Cuts at: 163 189 319 592 671 1235 1316 1424 1476
Size: 26 130 273 79 564 81 108 52
Cuts at: 1476 1683 2717 2939 3000 3050 3064 3169 3249
Size: 207 1034 222 61 50 14 105 80
Cuts at: 3249 3490 3791 3985 4063 4123 4825 5119 5730
Size: 241 301 194 78 60 702 294 611
Cuts at: 5730 5823 5897 6087 6149 6947 163
Size: 93 74 190 62 798 1321
Fragments arranged by size:
1321 1034 798 702 611 564 301 294
273 241 222 207 194 190 130 108
105 93 81 80 79 78 74 62
61 60 52 50 26 14
BspEI T'CCGG_A
Cuts at: 168 4007 168
Size: 3839 4266
BspHI T'CATG_A
Cuts at: 6537 6537
Size: 8105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 249 443 1452 2634 2746 4325 5155 5369 5671
Size: 194 1009 1182 112 1579 830 214 302
Cuts at: 5671 6052 249
Size: 381 2302
Fragments arranged by size:
2302 1579 1182 1009 830 381 302 214
194 112
BsrBI CCG'CTC
Cuts at: 50 248 4613 6397 6451 7935 8100 50
Size: 198 4365 1784 54 1484 165 55
Fragments arranged by size:
4365 1784 1484 198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 6017 6017
Size: 8105
BsrFI r'CCGG_y
Cuts at: 1413 2375 3752 6103 6284 1413
Size: 962 1377 2351 181 3234
Fragments arranged by size:
3234 2351 1377 962 181
BssHII G'CGCG_C
Cuts at: 115 115
Size: 8105
BssSI C'ACGA_G
Cuts at: 2236 6376 7084 2236
Size: 4140 708 3257
Fragments arranged by size:
4140 3257 708
BstAPI GCAn_nnn'nTGC
Cuts at: 264 606 4701 5482 264
Size: 342 4095 781 2887
Fragments arranged by size:
4095 2887 781 342
BstBI TT'CG_AA
Cuts at: 6466 6466
Size: 8105
BstEII G'GTnAC_C
Cuts at: 1320 1320
Size: 8105
BstXI CCAn_nnnn'nTGG
Cuts at: 939 2472 3633 939
Size: 1533 1161 5411
Fragments arranged by size:
5411 1533 1161
BstYI r'GATC_y
Cuts at: 1 506 717 1187 4197 5130 5597 5620 5955
Size: 505 211 470 3010 933 467 23 335
Cuts at: 5955 6201 6507 6519 6605 7657 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
3010 1052 933 505 470 467 449 335
306 246 211 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 950 1127 1619 4241 5092 950
Size: 177 492 2622 851 3963
Fragments arranged by size:
3963 2622 851 492 177
BtgI C'CryG_G
Cuts at: 107 189 3240 3957 107
Size: 82 3051 717 4255
Fragments arranged by size:
4255 3051 717 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1190 6055 6236 6399 1190
Size: 4865 181 163 2896
Fragments arranged by size:
4865 2896 181 163
BtsI GCAGTG_nn'
Cuts at: 201 373 843 992 1120 1889 2048 2204 2991
Size: 172 470 149 128 769 159 156 787
Cuts at: 2991 3844 4191 5348 201
Size: 853 347 1157 2958
Fragments arranged by size:
2958 1157 853 787 769 470 347 172
159 156 149 128
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1878 1913 2777 2812 3092 3127 1878
Size: 35 864 35 280 35 6856
Fragments arranged by size:
6856 864 280 35 35 35
DraI TTT'AAA
Cuts at: 5104 5104
Size: 8105
DraIII CAC_nnn'GTG
Cuts at: 3315 3315
Size: 8105
DrdI GACnn_nn'nnGTC
Cuts at: 1748 4003 5811 7155 1748
Size: 2255 1808 1344 2698
Fragments arranged by size:
2698 2255 1808 1344
EaeI y'GGCC_r
Cuts at: 863 876 3237 3773 3851 4761 4908 5690 5864
Size: 13 2361 536 78 910 147 782 174
Cuts at: 5864 6255 6282 7627 863
Size: 391 27 1345 1341
Fragments arranged by size:
2361 1345 1341 910 782 536 391 174
147 78 27 13
EagI C'GGCC_G
Cuts at: 5690 5690
Size: 8105
EarI CTCTTCn'nnn_
Cuts at: 716 765 845 1489 2405 3733 4551 4557 4578
Size: 49 80 644 916 1328 818 6 21
Cuts at: 4578 6128 6338 716
Size: 1550 210 2483
Fragments arranged by size:
2483 1550 1328 916 818 644 210 80
49 21 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4124 6313 7055 7201 7915 4124
Size: 2189 742 146 714 4314
Fragments arranged by size:
4314 2189 742 714 146
EcoICRI GAG'CTC
Cuts at: 1474 3247 5117 1474
Size: 1773 1870 4462
Fragments arranged by size:
4462 1870 1773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 801 855 1081 1165 1204 1309 1636 2129
Size: 317 54 226 84 39 105 327 493
Cuts at: 2129 2300 3101 3155 3389 3590 3655 3782 3916
Size: 171 801 54 234 201 65 127 134
Cuts at: 3916 3923 5930 6362 6715 7377 484
Size: 7 2007 432 353 662 1212
Fragments arranged by size:
2007 1212 801 662 493 432 353 327
317 234 226 201 171 134 127 105
84 65 54 54 39 7
EcoNI CCTnn'n_nnAGG
Cuts at: 424 424
Size: 8105
EcoO109I rG'GnC_Cy
Cuts at: 159 160 336 667 1765 2575 3047 3060 3067
Size: 1 176 331 1098 810 472 13 7
Cuts at: 3067 3743 4016 4475 5244 159
Size: 676 273 459 769 3020
Fragments arranged by size:
3020 1098 810 769 676 472 459 331
273 176 13 7 1
EcoRI G'AATT_C
Cuts at: 1707 5109 1707
Size: 3402 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3465 3555 4562 6124 3465
Size: 90 1007 1562 5446
Fragments arranged by size:
5446 1562 1007 90
FspI TGC'GCA
Cuts at: 5886 5886
Size: 8105
HaeII r_GCGC'y
Cuts at: 223 242 4083 5539 5787 7017 7788 223
Size: 19 3841 1456 248 1230 771 540
Fragments arranged by size:
3841 1456 1230 771 540 248 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 122 154 1333 1365 2233 2265 3198 3230 3333
Size: 32 1179 32 868 32 933 32 103
Cuts at: 3333 3365 3379 3411 4058 4090 4984 5016 5947
Size: 32 14 32 647 32 894 32 931
Cuts at: 5947 5977 5979 6009 122
Size: 30 2 30 2218
Fragments arranged by size:
2218 1179 933 931 894 868 647 103
32 32 32 32 32 32 32 32
30 30 14 2
HincII GTy'rAC
Cuts at: 657 1839 1869 1929 1944 2034 2121 2151 3723
Size: 1182 30 60 15 90 87 30 1572
Cuts at: 3723 5144 657
Size: 1421 3618
Fragments arranged by size:
3618 1572 1421 1182 90 87 60 30
30 15
HindIII A'AGCT_T
Cuts at: 769 769
Size: 8105
KasI G'GCGC_C
Cuts at: 5535 5783 5535
Size: 248 7857
KpnI G_GTAC'C
Cuts at: 2324 5125 2324
Size: 2801 5304
MluI A'CGCG_T
Cuts at: 7538 7538
Size: 8105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4433 5027 6864 7048 4433
Size: 594 1837 184 5490
Fragments arranged by size:
5490 1837 594 184
MscI TGG'CCA
Cuts at: 865 878 3239 3775 3853 4910 5866 865
Size: 13 2361 536 78 1057 956 3104
Fragments arranged by size:
3104 2361 1057 956 536 78 13
MslI CAynn'nnrTG
Cuts at: 4835 6094 6221 4835
Size: 1259 127 6719
Fragments arranged by size:
6719 1259 127
MspA1I CmG'CkG
Cuts at: 191 997 1171 1272 1696 3175 3931 4126 4305
Size: 806 174 101 424 1479 756 195 179
Cuts at: 4305 4514 5216 5530 5890 6674 6919 191
Size: 209 702 314 360 784 245 1377
Fragments arranged by size:
1479 1377 806 784 756 702 424 360
314 245 209 195 179 174 101
NaeI GCC'GGC
Cuts at: 6286 6286
Size: 8105
NarI GG'CG_CC
Cuts at: 5536 5784 5536
Size: 248 7857
NcoI C'CATG_G
Cuts at: 107 3240 3957 107
Size: 3133 717 4255
Fragments arranged by size:
4255 3133 717
NgoMIV G'CCGG_C
Cuts at: 6284 6284
Size: 8105
NsiI A_TGCA'T
Cuts at: 938 4147 938
Size: 3209 4896
NspI r_CATG'y
Cuts at: 2772 3038 3079 3359 5158 5658 7261 2772
Size: 266 41 280 1799 500 1603 3616
Fragments arranged by size:
3616 1799 1603 500 280 266 41
PasI CC'CwG_GG
Cuts at: 1397 1933 2211 3064 3434 1397
Size: 536 278 853 370 6068
Fragments arranged by size:
6068 853 536 370 278
PciI A'CATG_T
Cuts at: 3034 7257 3034
Size: 4223 3882
PflMI CCAn_nnn'nTGG
Cuts at: 997 997
Size: 8105
PfoI T'CCnGG_A
Cuts at: 920 1215 1235 920
Size: 295 20 7790
Fragments arranged by size:
7790 295 20
PmeI GTTT'AAAC
Cuts at: 5104 5104
Size: 8105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 626 658 6551 6583 626
Size: 32 5893 32 2148
Fragments arranged by size:
5893 2148 32 32
PpuMI rG'GwC_Cy
Cuts at: 336 2575 4016 4475 336
Size: 2239 1441 459 3966
Fragments arranged by size:
3966 2239 1441 459
PspOMI G'GGCC_C
Cuts at: 159 315 667 3046 3060 4119 159
Size: 156 352 2379 14 1059 4145
Fragments arranged by size:
4145 2379 1059 352 156 14
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3549 3581 3549
Size: 32 8073
PstI C_TGCA'G
Cuts at: 365 1896 2043 2531 2757 3936 4336 5152 5837
Size: 1531 147 488 226 1179 400 816 685
Cuts at: 5837 365
Size: 2633
Fragments arranged by size:
2633 1531 1179 816 685 488 400 226
147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8105
PvuII CAG'CTG
Cuts at: 997 1171 1272 1696 3175 3931 4126 4514 5530
Size: 174 101 424 1479 756 195 388 1016
Cuts at: 5530 5890 997
Size: 360 3212
Fragments arranged by size:
3212 1479 1016 756 424 388 360 195
174 101
RsrII CG'GwC_CG
Cuts at: 166 6300 166
Size: 6134 1971
SacI G_AGCT'C
Cuts at: 1476 3249 5119 1476
Size: 1773 1870 4462
Fragments arranged by size:
4462 1870 1773
SacII CC_GC'GG
Cuts at: 192 192
Size: 8105
SalI G'TCGA_C
Cuts at: 1837 5142 1837
Size: 3305 4800
SapI GCTCTTCn'nnn_
Cuts at: 1489 4551 6128 6338 1489
Size: 3062 1577 210 3256
Fragments arranged by size:
3256 3062 1577 210
SbfI CC_TGCA'GG
Cuts at: 2757 4336 5152 2757
Size: 1579 816 5710
Fragments arranged by size:
5710 1579 816
ScaI AGT'ACT
Cuts at: 2834 7622 2834
Size: 4788 3317
SexAI A'CCwGG_T
Cuts at: 1458 1458
Size: 8105
SfcI C'TryA_G
Cuts at: 33 361 1571 1892 2039 2527 2570 2753 3701
Size: 328 1210 321 147 488 43 183 948
Cuts at: 3701 3932 4332 4515 5148 5833 6801 6992 33
Size: 231 400 183 633 685 968 191 1146
Fragments arranged by size:
1210 1146 968 948 685 633 488 400
328 321 231 191 183 183 147 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 4376 4376
Size: 8105
SfoI GGC'GCC
Cuts at: 5537 5785 5537
Size: 248 7857
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8105
SmaI CCC'GGG
Cuts at: 583 1672 4159 5127 583
Size: 1089 2487 968 3561
Fragments arranged by size:
3561 2487 1089 968
SmlI C'TyrA_G
Cuts at: 1084 2551 4350 5260 6175 6889 7151 1084
Size: 1467 1799 910 915 714 262 2038
Fragments arranged by size:
2038 1799 1467 915 910 714 262
SpeI A'CTAG_T
Cuts at: 67 1255 67
Size: 1188 6917
SphI G_CATG'C
Cuts at: 2772 5158 5658 2772
Size: 2386 500 5219
Fragments arranged by size:
5219 2386 500
StuI AGG'CCT
Cuts at: 4246 4246
Size: 8105
StyI C'CwwG_G
Cuts at: 107 441 1316 1424 1700 2252 2992 3050 3240
Size: 334 875 108 276 552 740 58 190
Cuts at: 3240 3266 3461 3478 3957 4115 4842 5239 5593
Size: 26 195 17 479 158 727 397 354
Cuts at: 5593 107
Size: 2619
Fragments arranged by size:
2619 875 740 727 552 479 397 354
334 276 195 190 158 108 58 26
17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1061 1084 3530 4457 5688 5821 6280 6489 1061
Size: 23 2446 927 1231 133 459 209 2677
Fragments arranged by size:
2677 2446 1231 927 459 209 133 23
TatI w'GTAC_w
Cuts at: 673 2832 3192 7620 673
Size: 2159 360 4428 1158
Fragments arranged by size:
4428 2159 1158 360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 4170 4202 4263 4295 7622 7654 2216
Size: 32 1922 32 61 32 3327 32 2667
Fragments arranged by size:
3327 2667 1922 61 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 136 5902 136
Size: 5766 2339
XbaI T'CTAG_A
Cuts at: 1770 5136 1770
Size: 3366 4739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 223 722 1404 1965 2438 3982 4496 5600 223
Size: 499 682 561 473 1544 514 1104 2728
Fragments arranged by size:
2728 1544 1104 682 561 514 499 473
XmaI C'CCGG_G
Cuts at: 581 1670 4157 5125 581
Size: 1089 2487 968 3561
Fragments arranged by size:
3561 2487 1089 968
XmnI GAAnn'nnTTC
Cuts at: 1628 1628
Size: 8105
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AvrII BmtI
BsiWI BsrGI BstZ17I ClaI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PmlI PshAI
PsiI PspXI SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI