Restriction map for
pF1KA0591
(Circular) (Six-base) MAPSORT of: pF1KA0591.seq Check: 1637 from: 1 to: 8561
pF1KA0591 8561 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 1474 1474
Size: 8561
AccI GT'mk_AC
Cuts at: 1504 5024 5599 1504
Size: 3520 575 4466
Fragments arranged by size:
4466 3520 575
Acc65I G'GTAC_C
Cuts at: 5577 5577
Size: 8561
AclI AA'CG_TT
Cuts at: 1533 8401 1533
Size: 6868 1693
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1264 2312 2958 3440 4159 6386 6818 7565
Size: 1024 1048 646 482 719 2227 432 747
Cuts at: 7565 7833 240
Size: 268 968
Fragments arranged by size:
2227 1048 1024 968 747 719 646 482
432 268
AfeI AGC'GCT
Cuts at: 1912 1912
Size: 8561
AflIII A'CryG_T
Cuts at: 5913 7017 7994 5913
Size: 1104 977 6480
Fragments arranged by size:
6480 1104 977
AgeI A'CCGG_T
Cuts at: 6949 6949
Size: 8561
AhdI GACnn_n'nnGTC
Cuts at: 3246 3246
Size: 8561
AleI CACnn'nnGTG
Cuts at: 2801 2801
Size: 8561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 364 422 3638 364
Size: 58 3216 5287
Fragments arranged by size:
5287 3216 58
AlwNI CAG_nnn'CTG
Cuts at: 1269 1795 2220 2652 4925 5258 5866 7433 1269
Size: 526 425 432 2273 333 608 1567 2397
Fragments arranged by size:
2397 2273 1567 608 526 432 425 333
ApaLI G'TGCA_C
Cuts at: 4906 7331 4906
Size: 2425 6136
ApoI r'AATT_y
Cuts at: 551 1059 2335 3182 4894 5095 5161 5565 7914
Size: 508 1276 847 1712 201 66 404 2349
Cuts at: 7914 8170 551
Size: 256 942
Fragments arranged by size:
2349 1712 1276 942 847 508 404 256
201 66
AseI AT'TA_AT
Cuts at: 20 920 3356 20
Size: 900 2436 5225
Fragments arranged by size:
5225 2436 900
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8561
AvaI C'yCGr_G
Cuts at: 158 4337 5072 5144 5538 5581 158
Size: 4179 735 72 394 43 3138
Fragments arranged by size:
4179 3138 735 394 72 43
AvrII C'CTAG_G
Cuts at: 1674 1674
Size: 8561
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4496 4529 4496
Size: 33 8528
BamHI G'GATC_C
Cuts at: 4959 5586 4959
Size: 627 7934
BanI G'GyrC_C
Cuts at: 883 1802 5577 5991 6239 6274 883
Size: 919 3775 414 248 35 3170
Fragments arranged by size:
3775 3170 919 414 248 35
BanII G_rGCy'C
Cuts at: 1105 1830 1904 2785 3569 4025 4610 5575 6605
Size: 725 74 881 784 456 585 965 1030
Cuts at: 6605 1105
Size: 3061
Fragments arranged by size:
3061 1030 965 881 784 725 585 456
74
BbeI G_GCGC'C
Cuts at: 1806 5995 6243 1806
Size: 4189 248 4124
Fragments arranged by size:
4189 4124 248
BbsI GAAGACnn'nnnn_
Cuts at: 1730 2050 4136 4320 5059 5289 5401 1730
Size: 320 2086 184 739 230 112 4890
Fragments arranged by size:
4890 2086 739 320 230 184 112
BbvCI CC'TCA_GC
Cuts at: 3746 4708 4723 3746
Size: 962 15 7584
Fragments arranged by size:
7584 962 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2800 2834 2800
Size: 34 8527
BciVI GTATCCnnnnn_n'
Cuts at: 3071 5218 6459 7220 8561 3071
Size: 2147 1241 761 1341 3071
Fragments arranged by size:
3071 2147 1341 1241 761
BclI T'GATC_A
Cuts at: 6081 6081
Size: 8561
BfrBI ATG'CAT
Cuts at: 185 185
Size: 8561
BglI GCCn_nnn'nGGC
Cuts at: 455 2353 4382 455
Size: 1898 2029 4634
Fragments arranged by size:
4634 2029 1898
BglII A'GATC_T
Cuts at: 2165 2196 2967 4247 4349 4751 6076 8113 2165
Size: 31 771 1280 102 402 1325 2037 2613
Fragments arranged by size:
2613 2037 1325 1280 771 402 102 31
BlpI GC'TnA_GC
Cuts at: 1796 1819 2033 5673 1796
Size: 23 214 3640 4684
Fragments arranged by size:
4684 3640 214 23
Bme1580I G_kGCm'C
Cuts at: 2706 3071 4910 6186 6279 7335 2706
Size: 365 1839 1276 93 1056 3932
Fragments arranged by size:
3932 1839 1276 1056 365 93
BmrI ACTGGGnnnn_n'
Cuts at: 3454 5268 5773 5854 6189 8333 3454
Size: 1814 505 81 335 2144 3682
Fragments arranged by size:
3682 2144 1814 505 335 81
BmtI G_CTAG'C
Cuts at: 1372 1372
Size: 8561
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1844 2082 2712 4585 5057 6928 1844
Size: 238 630 1873 472 1871 3477
Fragments arranged by size:
3477 1873 1871 630 472 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 2682 3320 3910 4615 4624 1532
Size: 1150 638 590 705 9 5469
Fragments arranged by size:
5469 1150 705 638 590 9
Bpu10I CC'TnA_GC
Cuts at: 1262 1965 3046 3746 4684 4708 4723 1262
Size: 703 1081 700 938 24 15 5100
Fragments arranged by size:
5100 1081 938 703 700 24 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2626 4597 5737 6616 7108 7406 2626
Size: 1971 1140 879 492 298 3781
Fragments arranged by size:
3781 1971 1140 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 157 284 799 2053 5217 8265 157
Size: 127 515 1254 3164 3048 453
Fragments arranged by size:
3164 3048 1254 515 453 127
BsaAI yAC'GTr
Cuts at: 1926 5914 1926
Size: 3988 4573
BsaBI GATnn'nnATC
Cuts at: 6 1289 4412 6075 8112 6
Size: 1283 3123 1663 2037 455
Fragments arranged by size:
3123 2037 1663 1283 455
BsaHI Gr'CG_yC
Cuts at: 1471 1803 2933 5992 6240 6942 8438 1471
Size: 332 1130 3059 248 702 1496 1594
Fragments arranged by size:
3059 1594 1496 1130 702 332 248
BsaWI w'CCGG_w
Cuts at: 1856 2360 5750 6271 6949 7223 7370 7781 1856
Size: 504 3390 521 678 274 147 411 2636
Fragments arranged by size:
3390 2636 678 521 504 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 107 137 634 664 4980 5010 107
Size: 30 497 30 4316 30 3658
Fragments arranged by size:
4316 3658 497 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1243 1529 2311 2971 3298 3323 4734 4992 1243
Size: 286 782 660 327 25 1411 258 4812
Fragments arranged by size:
4812 1411 782 660 327 286 258 25
BseYI C'CCAG_C
Cuts at: 700 1946 3912 4702 5118 5986 6744 7321 7817
Size: 1246 1966 790 416 868 758 577 496
Cuts at: 7817 700
Size: 1444
Fragments arranged by size:
1966 1444 1246 868 790 758 577 496
416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 963 2488 3634 5403 963
Size: 1525 1146 1769 4121
Fragments arranged by size:
4121 1769 1525 1146
BsiEI CG_ry'CG
Cuts at: 104 2826 2855 3003 6103 6149 7357 104
Size: 2722 29 148 3100 46 1208 1308
Fragments arranged by size:
3100 2722 1308 1208 148 46 29
BsiHKAI G_wGCw'C
Cuts at: 1138 1263 2785 4025 4583 4610 4910 5575 6353
Size: 125 1522 1240 558 27 300 665 778
Cuts at: 6353 6543 7335 1138
Size: 190 792 2364
Fragments arranged by size:
2364 1522 1240 792 778 665 558 300
190 125 27
BsmI GAATG_Cn'
Cuts at: 2920 3553 2920
Size: 633 7928
Bsp1286I G_dGCh'C
Cuts at: 1105 1138 1263 1830 1904 2706 2785 3071 3569
Size: 33 125 567 74 802 79 286 498
Cuts at: 3569 4025 4583 4610 4910 5575 6186 6279 6353
Size: 456 558 27 300 665 611 93 74
Cuts at: 6353 6543 6605 7335 1105
Size: 190 62 730 2331
Fragments arranged by size:
2331 802 730 665 611 567 558 498
456 300 286 190 125 93 79 74
74 62 33 27
BspEI T'CCGG_A
Cuts at: 1856 2360 7781 1856
Size: 504 5421 2636
Fragments arranged by size:
5421 2636 504
BspHI T'CATG_A
Cuts at: 2550 7737 2550
Size: 5187 3374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2247 5611 5825 6127 6508 2247
Size: 3364 214 302 381 4300
Fragments arranged by size:
4300 3364 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1800 1823 6853 6907 8391 8556 50
Size: 1750 23 5030 54 1484 165 55
Fragments arranged by size:
5030 1750 1484 165 55 54 23
BsrDI GCAATG_nn'
Cuts at: 680 1091 1182 1556 2418 3263 4284 6473 680
Size: 411 91 374 862 845 1021 2189 2768
Fragments arranged by size:
2768 2189 1021 862 845 411 374 91
BsrFI r'CCGG_y
Cuts at: 2935 4296 6559 6740 6949 2935
Size: 1361 2263 181 209 4547
Fragments arranged by size:
4547 2263 1361 209 181
BsrGI T'GTAC_A
Cuts at: 326 3135 326
Size: 2809 5752
BssSI C'ACGA_G
Cuts at: 2027 6832 7190 2027
Size: 4805 358 3398
Fragments arranged by size:
4805 3398 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1090 1156 2247 4099 5938 1090
Size: 66 1091 1852 1839 3713
Fragments arranged by size:
3713 1852 1839 1091 66
BstBI TT'CG_AA
Cuts at: 4411 6922 4411
Size: 2511 6050
BstXI CCAn_nnnn'nTGG
Cuts at: 720 1614 2448 2538 720
Size: 894 834 90 6743
Fragments arranged by size:
6743 894 834 90
BstYI r'GATC_y
Cuts at: 1 297 645 849 2165 2196 2967 4247 4349
Size: 296 348 204 1316 31 771 1280 102
Cuts at: 4349 4751 4959 5586 6053 6076 6411 6657 7669
Size: 402 208 627 467 23 335 246 1012
Cuts at: 7669 7755 7767 8113 1
Size: 86 12 346 449
Fragments arranged by size:
1316 1280 1012 771 627 467 449 402
348 346 335 296 246 208 204 102
86 31 23 12
Bsu36I CC'TnA_GG
Cuts at: 4076 4076
Size: 8561
BtgI C'CryG_G
Cuts at: 1106 1412 2796 2849 3560 4557 5387 5430 1106
Size: 306 1384 53 711 997 830 43 4237
Fragments arranged by size:
4237 1384 997 830 711 306 53 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3129 3523 6511 6692 6855 3129
Size: 394 2988 181 163 4835
Fragments arranged by size:
4835 2988 394 181 163
BtsI GCAGTG_nn'
Cuts at: 3456 3632 4719 4803 4989 5804 3456
Size: 176 1087 84 186 815 6213
Fragments arranged by size:
6213 1087 815 186 176 84
ClaI AT'CG_AT
Cuts at: 1301 1301
Size: 8561
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4749 4784 5189 5224 4749
Size: 35 405 35 8086
Fragments arranged by size:
8086 405 35 35
DraI TTT'AAA
Cuts at: 1538 5560 1538
Size: 4022 4539
DraIII CAC_nnn'GTG
Cuts at: 765 3084 765
Size: 2319 6242
DrdI GACnn_nn'nnGTC
Cuts at: 6267 7125 6267
Size: 858 7703
EaeI y'GGCC_r
Cuts at: 962 1392 1782 2852 3086 3837 6146 6320 6711
Size: 430 390 1070 234 751 2309 174 391
Cuts at: 6711 6738 8083 962
Size: 27 1345 1440
Fragments arranged by size:
2309 1440 1345 1070 751 430 391 390
234 174 27
EagI C'GGCC_G
Cuts at: 2852 6146 2852
Size: 3294 5267
EarI CTCTTCn'nnn_
Cuts at: 1577 1835 2288 2300 2977 3416 4526 6584 6794
Size: 258 453 12 677 439 1110 2058 210
Cuts at: 6794 1577
Size: 3344
Fragments arranged by size:
3344 2058 1110 677 453 439 258 210
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3731 6769 7079 7225 8371 3731
Size: 3038 310 146 1146 3921
Fragments arranged by size:
3921 3038 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2783 4023 4608 5573 2783
Size: 1240 585 965 5771
Fragments arranged by size:
5771 1240 965 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1264 1532 2312 2682 2958 3320 3440 3910
Size: 1024 268 780 370 276 362 120 470
Cuts at: 3910 4159 4615 4624 6386 6818 7565 7833 240
Size: 249 456 9 1762 432 747 268 968
Fragments arranged by size:
1762 1024 968 780 747 470 456 432
370 362 276 268 268 249 120 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2428 4615 5443 2428
Size: 2187 828 5546
Fragments arranged by size:
5546 2187 828
EcoO109I rG'GnC_Cy
Cuts at: 146 1194 1248 1266 1418 4163 4718 5436 5448
Size: 1048 54 18 152 2745 555 718 12
Cuts at: 5448 5700 146
Size: 252 3007
Fragments arranged by size:
3007 2745 1048 718 555 252 152 54
18 12
EcoRI G'AATT_C
Cuts at: 3182 4894 5565 3182
Size: 1712 671 6178
Fragments arranged by size:
6178 1712 671
EcoRV GAT'ATC
Cuts at: 1113 3513 4941 1113
Size: 2400 1428 4733
Fragments arranged by size:
4733 2400 1428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1606 2215 5245 6580 1606
Size: 609 3030 1335 3587
Fragments arranged by size:
3587 3030 1335 609
FspI TGC'GCA
Cuts at: 6342 6342
Size: 8561
HaeII r_GCGC'y
Cuts at: 1806 1914 2817 4778 5995 6243 7265 8244 1806
Size: 108 903 1961 1217 248 1022 979 2123
Fragments arranged by size:
2123 1961 1217 1022 979 903 248 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 362 394 634 666 1294 1326 1689 1721 2923
Size: 32 240 32 628 32 363 32 1202
Cuts at: 2923 2947 2954 2955 2979 2986 3160 3192 3232
Size: 24 7 1 24 7 174 32 40
Cuts at: 3232 3264 3358 3390 3893 3925 4232 4264 4681
Size: 32 94 32 503 32 307 32 417
Cuts at: 4681 4713 6403 6433 6435 6465 362
Size: 32 1690 30 2 30 2458
Fragments arranged by size:
2458 1690 1202 628 503 417 363 307
240 174 94 40 32 32 32 32
32 32 32 32 32 32 30 30
24 24 7 7 2 1
HincII GTy'rAC
Cuts at: 1713 3924 5600 1713
Size: 2211 1676 4674
Fragments arranged by size:
4674 2211 1676
HindIII A'AGCT_T
Cuts at: 1588 5521 1588
Size: 3933 4628
HpaI GTT'AAC
Cuts at: 1713 1713
Size: 8561
KasI G'GCGC_C
Cuts at: 1802 5991 6239 1802
Size: 4189 248 4124
Fragments arranged by size:
4189 4124 248
KpnI G_GTAC'C
Cuts at: 5581 5581
Size: 8561
MfeI C'AATT_G
Cuts at: 4949 4949
Size: 8561
MluI A'CGCG_T
Cuts at: 7994 7994
Size: 8561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2885 7232 7416 2885
Size: 4347 184 4030
Fragments arranged by size:
4347 4030 184
MscI TGG'CCA
Cuts at: 1394 1784 3088 3839 6322 1394
Size: 390 1304 751 2483 3633
Fragments arranged by size:
3633 2483 1304 751 390
MslI CAynn'nnrTG
Cuts at: 193 471 800 1612 2446 2536 2801 3022 3542
Size: 278 329 812 834 90 265 221 520
Cuts at: 3542 6550 6677 193
Size: 3008 127 2077
Fragments arranged by size:
3008 2077 834 812 520 329 278 265
221 127 90
MspA1I CmG'CkG
Cuts at: 1108 1877 1959 3151 3916 4388 5122 5672 5986
Size: 769 82 1192 765 472 734 550 314
Cuts at: 5986 6346 7359 7604 1108
Size: 360 1013 245 2065
Fragments arranged by size:
2065 1192 1013 769 765 734 550 472
360 314 245 82
NaeI GCC'GGC
Cuts at: 2937 4298 6742 2937
Size: 1361 2444 4756
Fragments arranged by size:
4756 2444 1361
NarI GG'CG_CC
Cuts at: 1803 5992 6240 1803
Size: 4189 248 4124
Fragments arranged by size:
4189 4124 248
NcoI C'CATG_G
Cuts at: 1412 5387 1412
Size: 3975 4586
NdeI CA'TA_TG
Cuts at: 4518 4518
Size: 8561
NgoMIV G'CCGG_C
Cuts at: 2935 4296 6740 2935
Size: 1361 2444 4756
Fragments arranged by size:
4756 2444 1361
NheI G'CTAG_C
Cuts at: 1368 1368
Size: 8561
NsiI A_TGCA'T
Cuts at: 187 187
Size: 8561
NspI r_CATG'y
Cuts at: 5614 6114 7021 5614
Size: 500 907 7154
Fragments arranged by size:
7154 907 500
PciI A'CATG_T
Cuts at: 7017 7017
Size: 8561
PflMI CCAn_nnn'nTGG
Cuts at: 628 3084 628
Size: 2456 6105
PfoI T'CCnGG_A
Cuts at: 2594 2594
Size: 8561
PmeI GTTT'AAAC
Cuts at: 5560 5560
Size: 8561
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7700 7732 7700
Size: 32 8529
PpuMI rG'GwC_Cy
Cuts at: 1194 1248 1418 4163 4718 5436 1194
Size: 54 170 2745 555 718 4319
Fragments arranged by size:
4319 2745 718 555 170 54
PstI C_TGCA'G
Cuts at: 2283 4095 5608 6293 2283
Size: 1812 1513 685 4551
Fragments arranged by size:
4551 1812 1513 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8561
PvuII CAG'CTG
Cuts at: 1959 3916 5122 5986 6346 1959
Size: 1957 1206 864 360 4174
Fragments arranged by size:
4174 1957 1206 864 360
RsrII CG'GwC_CG
Cuts at: 6756 6756
Size: 8561
SacI G_AGCT'C
Cuts at: 2785 4025 4610 5575 2785
Size: 1240 585 965 5771
Fragments arranged by size:
5771 1240 965 585
SacII CC_GC'GG
Cuts at: 1109 1109
Size: 8561
SalI G'TCGA_C
Cuts at: 5598 5598
Size: 8561
SanDI GG'GwC_CC
Cuts at: 1418 4718 1418
Size: 3300 5261
SapI GCTCTTCn'nnn_
Cuts at: 1835 6584 6794 1835
Size: 4749 210 3602
Fragments arranged by size:
4749 3602 210
SbfI CC_TGCA'GG
Cuts at: 5608 5608
Size: 8561
ScaI AGT'ACT
Cuts at: 8078 8078
Size: 8561
SfcI C'TryA_G
Cuts at: 33 530 1023 2152 2279 3896 4091 5604 6289
Size: 497 493 1129 127 1617 195 1513 685
Cuts at: 6289 7282 7473 33
Size: 993 191 1121
Fragments arranged by size:
1617 1513 1129 1121 993 685 497 493
195 191 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 2353 2353
Size: 8561
SfoI GGC'GCC
Cuts at: 1804 5993 6241 1804
Size: 4189 248 4124
Fragments arranged by size:
4189 4124 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8561
SmaI CCC'GGG
Cuts at: 5074 5583 5074
Size: 509 8052
SmlI C'TyrA_G
Cuts at: 158 2641 4576 5716 6631 7123 7385 158
Size: 2483 1935 1140 915 492 262 1334
Fragments arranged by size:
2483 1935 1334 1140 915 492 262
SnaBI TAC'GTA
Cuts at: 1926 1926
Size: 8561
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8561
SphI G_CATG'C
Cuts at: 5614 6114 5614
Size: 500 8061
SspI AAT'ATT
Cuts at: 918 3621 4119 918
Size: 2703 498 5360
Fragments arranged by size:
5360 2703 498
StuI AGG'CCT
Cuts at: 2245 2562 2245
Size: 317 8244
StyI C'CwwG_G
Cuts at: 959 1412 1674 2320 3010 3278 3834 4310 5387
Size: 453 262 646 690 268 556 476 1077
Cuts at: 5387 5695 6049 959
Size: 308 354 3471
Fragments arranged by size:
3471 1077 690 646 556 476 453 354
308 268 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 635 799 3017 3722 3789 4850 4891 6144 6277
Size: 164 2218 705 67 1061 41 1253 133
Cuts at: 6277 6736 6945 635
Size: 459 209 2251
Fragments arranged by size:
2251 2218 1253 1061 705 459 209 164
133 67 41
TatI w'GTAC_w
Cuts at: 326 1035 2501 3135 5366 8076 326
Size: 709 1466 634 2231 2710 811
Fragments arranged by size:
2710 2231 1466 811 709 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 107 139 623 655 4452 4484 4980 5012 8078
Size: 32 484 32 3797 32 496 32 3066
Cuts at: 8078 8110 107
Size: 32 558
Fragments arranged by size:
3797 3066 558 496 484 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3308 4260 6358 3308
Size: 952 2098 5511
Fragments arranged by size:
5511 2098 952
XbaI T'CTAG_A
Cuts at: 5592 5592
Size: 8561
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1206 1410 5358 6056 1206
Size: 204 3948 698 3711
Fragments arranged by size:
3948 3711 698 204
XhoI C'TCGA_G
Cuts at: 158 158
Size: 8561
XmaI C'CCGG_G
Cuts at: 5072 5581 5072
Size: 509 8052
XmnI GAAnn'nnTTC
Cuts at: 2315 2525 5105 2315
Size: 210 2580 5771
Fragments arranged by size:
5771 2580 210
ZraI GAC'GTC
Cuts at: 1472 1472
Size: 8561
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AloI ApaI AscI BmgBI BsiWI BsmBI
BssHII BstEII BstZ17I FseI FspAI NotI NruI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SexAI
SgrAI SrfI SwaI