Restriction map for
pF1KA0590
(Circular) (Six-base) MAPSORT of: pF1KA0590.seq Check: 5411 from: 1 to: 7499
pF1KA0590 7499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1485 2813 1485
Size: 1328 6171
AatII G_ACGT'C
Cuts at: 3469 4241 3469
Size: 772 6727
AccI GT'mk_AC
Cuts at: 4537 4537
Size: 7499
Acc65I G'GTAC_C
Cuts at: 1132 4515 1132
Size: 3383 4116
AclI AA'CG_TT
Cuts at: 1586 7339 1586
Size: 5753 1746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1204 2427 3784 5324 5756 6109 6771 712
Size: 492 1223 1357 1540 432 353 662 1440
Fragments arranged by size:
1540 1440 1357 1223 662 492 432 353
AfeI AGC'GCT
Cuts at: 2612 2612
Size: 7499
AflIII A'CryG_T
Cuts at: 276 653 4337 4851 6651 6932 276
Size: 377 3684 514 1800 281 843
Fragments arranged by size:
3684 1800 843 514 377 281
AhdI GACnn_n'nnGTC
Cuts at: 2934 2934
Size: 7499
AleI CACnn'nnGTG
Cuts at: 781 1445 781
Size: 664 6835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1453 3717 1453
Size: 2264 5235
AlwNI CAG_nnn'CTG
Cuts at: 813 1013 2556 3000 3475 3876 4804 6242 813
Size: 200 1543 444 475 401 928 1438 2070
Fragments arranged by size:
2070 1543 1438 928 475 444 401 200
ApaI G_GGCC'C
Cuts at: 1218 2607 2868 3942 1218
Size: 1389 261 1074 4775
Fragments arranged by size:
4775 1389 1074 261
ApaLI G'TGCA_C
Cuts at: 748 2800 3196 4375 6337 748
Size: 2052 396 1179 1962 1910
Fragments arranged by size:
2052 1962 1910 1179 396
ApoI r'AATT_y
Cuts at: 1701 4503 6852 7108 1701
Size: 2802 2349 256 2092
Fragments arranged by size:
2802 2349 2092 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7499
AvaI C'yCGr_G
Cuts at: 1189 1377 2423 2606 3050 3209 3298 4414 4519
Size: 188 1046 183 444 159 89 1116 105
Cuts at: 4519 1189
Size: 4169
Fragments arranged by size:
4169 1116 1046 444 188 183 159 105
89
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 263 296 263
Size: 33 7466
BamHI G'GATC_C
Cuts at: 4524 4524
Size: 7499
BanI G'GyrC_C
Cuts at: 176 314 882 1132 2345 2834 2907 4515 4929
Size: 138 568 250 1213 489 73 1608 414
Cuts at: 4929 5177 5212 176
Size: 248 35 2463
Fragments arranged by size:
2463 1608 1213 568 489 414 250 248
138 73 35
BanII G_rGCy'C
Cuts at: 445 1218 1238 2081 2607 2868 2948 3295 3942
Size: 773 20 843 526 261 80 347 647
Cuts at: 3942 4072 4357 4513 5543 445
Size: 130 285 156 1030 2401
Fragments arranged by size:
2401 1030 843 773 647 526 347 285
261 156 130 80 20
BbeI G_GCGC'C
Cuts at: 4933 5181 4933
Size: 248 7251
BbsI GAAGACnn'nnnn_
Cuts at: 1111 2333 2551 1111
Size: 1222 218 6059
Fragments arranged by size:
6059 1222 218
BbvCI CC'TCA_GC
Cuts at: 1318 1318
Size: 7499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 792 826 960 994 2776 2810 792
Size: 34 134 34 1782 34 5481
Fragments arranged by size:
5481 1782 134 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5397 6453 7499 5397
Size: 1056 1046 5397
Fragments arranged by size:
5397 1056 1046
BclI T'GATC_A
Cuts at: 5019 5019
Size: 7499
BfrBI ATG'CAT
Cuts at: 1575 1575
Size: 7499
BglI GCCn_nnn'nGGC
Cuts at: 2602 2653 2662 3775 4021 2602
Size: 51 9 1113 246 6080
Fragments arranged by size:
6080 1113 246 51 9
BglII A'GATC_T
Cuts at: 5014 7051 5014
Size: 2037 5462
BlpI GC'TnA_GC
Cuts at: 925 3289 4611 925
Size: 2364 1322 3813
Fragments arranged by size:
3813 2364 1322
Bme1580I G_kGCm'C
Cuts at: 752 1218 2607 2804 2837 2868 2912 3200 3216
Size: 466 1389 197 33 31 44 288 16
Cuts at: 3216 3942 3978 4379 5124 5217 6341 752
Size: 726 36 401 745 93 1124 1910
Fragments arranged by size:
1910 1389 1124 745 726 466 401 288
197 93 44 36 33 31 16
BmgBI CAC'GTC
Cuts at: 279 2562 279
Size: 2283 5216
BmrI ACTGGGnnnn_n'
Cuts at: 440 4711 4792 5127 7271 440
Size: 4271 81 335 2144 668
Fragments arranged by size:
4271 2144 668 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5866 5866
Size: 7499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 374 1538 2422 2704 2758 2860 2962 3022
Size: 106 1164 884 282 54 102 102 60
Cuts at: 3022 3421 3703 4219 4324 268
Size: 399 282 516 105 3443
Fragments arranged by size:
3443 1164 884 516 399 282 282 106
105 102 102 60 54
Bpu10I CC'TnA_GC
Cuts at: 1318 1318
Size: 7499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4675 5554 6268 6566 4675
Size: 879 714 298 5608
Fragments arranged by size:
5608 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 707 1377 2370 4078 4396 7203 707
Size: 670 993 1708 318 2807 1003
Fragments arranged by size:
2807 1708 1003 993 670 318
BsaAI yAC'GTr
Cuts at: 2952 4266 4350 4852 2952
Size: 1314 84 502 5599
Fragments arranged by size:
5599 1314 502 84
BsaBI GATnn'nnATC
Cuts at: 6 5013 7050 6
Size: 5007 2037 455
Fragments arranged by size:
5007 2037 455
BsaHI Gr'CG_yC
Cuts at: 529 1558 2113 2429 2690 3023 3466 4238 4370
Size: 1029 555 316 261 333 443 772 132
Cuts at: 4370 4445 4930 5178 5880 7376 529
Size: 75 485 248 702 1496 652
Fragments arranged by size:
1496 1029 772 702 652 555 485 443
333 316 261 248 132 75
BsaWI w'CCGG_w
Cuts at: 3790 4688 5209 5887 6298 6445 6719 3790
Size: 898 521 678 411 147 274 4570
Fragments arranged by size:
4570 898 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 432 462 870 900 1634 1664 2329 2359 3046
Size: 30 408 30 734 30 665 30 687
Cuts at: 3046 3076 432
Size: 30 4855
Fragments arranged by size:
4855 734 687 665 408 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1043 1639 3193 3346 3625 3636 3676 4321 4477
Size: 596 1554 153 279 11 40 645 156
Cuts at: 4477 1043
Size: 4065
Fragments arranged by size:
4065 1554 645 596 279 156 153 40
11
BseYI C'CCAG_C
Cuts at: 344 1870 2123 2549 2673 3419 4386 4924 5682
Size: 1526 253 426 124 746 967 538 758
Cuts at: 5682 6347 6755 344
Size: 665 408 1088
Fragments arranged by size:
1526 1088 967 758 746 665 538 426
408 253 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1556 1857 2125 3016 3364 1556
Size: 301 268 891 348 5691
Fragments arranged by size:
5691 891 348 301 268
BsiEI CG_ry'CG
Cuts at: 104 942 2796 5041 5087 6317 104
Size: 838 1854 2245 46 1230 1286
Fragments arranged by size:
2245 1854 1286 1230 838 46
BsiHKAI G_wGCw'C
Cuts at: 434 752 1238 1855 2216 2789 2804 2948 3200
Size: 318 486 617 361 573 15 144 252
Cuts at: 3200 3295 3515 4262 4379 4513 5291 5481 6341
Size: 95 220 747 117 134 778 190 860
Cuts at: 6341 434
Size: 1592
Fragments arranged by size:
1592 860 778 747 617 573 486 361
318 252 220 190 144 134 117 95
15
BsmI GAATG_Cn'
Cuts at: 3234 3234
Size: 7499
BsmBI CGTCTCn'nnnn_
Cuts at: 1549 1969 2104 3457 1549
Size: 420 135 1353 5591
Fragments arranged by size:
5591 1353 420 135
Bsp1286I G_dGCh'C
Cuts at: 434 445 752 1218 1238 1855 2081 2216 2607
Size: 11 307 466 20 617 226 135 391
Cuts at: 2607 2789 2804 2837 2868 2912 2948 3200 3216
Size: 182 15 33 31 44 36 252 16
Cuts at: 3216 3295 3515 3942 3978 4072 4262 4357 4379
Size: 79 220 427 36 94 190 95 22
Cuts at: 4379 4513 5124 5217 5291 5481 5543 6341 434
Size: 134 611 93 74 190 62 798 1592
Fragments arranged by size:
1592 798 617 611 466 427 391 307
252 226 220 190 190 182 135 134
95 94 93 79 74 62 44 36
36 33 31 22 20 16 15 11
BspEI T'CCGG_A
Cuts at: 3790 3790
Size: 7499
BspHI T'CATG_A
Cuts at: 2229 3272 3902 5931 2229
Size: 1043 630 2029 3797
Fragments arranged by size:
3797 2029 1043 630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1289 1359 1485 2813 2939 3662 3875 4410 4549
Size: 70 126 1328 126 723 213 535 139
Cuts at: 4549 4763 5065 5446 1289
Size: 214 302 381 3342
Fragments arranged by size:
3342 1328 723 535 381 302 214 213
139 126 126 70
BsrBI CCG'CTC
Cuts at: 50 632 929 1326 5791 5845 7329 7494 50
Size: 582 297 397 4465 54 1484 165 55
Fragments arranged by size:
4465 1484 582 397 297 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1577 3099 5411 1577
Size: 1522 2312 3665
Fragments arranged by size:
3665 2312 1522
BsrFI r'CCGG_y
Cuts at: 937 2756 3751 5497 5678 937
Size: 1819 995 1746 181 2758
Fragments arranged by size:
2758 1819 1746 995 181
BsrGI T'GTAC_A
Cuts at: 875 2702 875
Size: 1827 5672
BssHII G'CGCG_C
Cuts at: 2160 2530 2160
Size: 370 7129
BssSI C'ACGA_G
Cuts at: 544 706 979 5770 6478 544
Size: 162 273 4791 708 1565
Fragments arranged by size:
4791 1565 708 273 162
BstAPI GCAn_nnn'nTGC
Cuts at: 399 3080 3195 4266 4876 399
Size: 2681 115 1071 610 3022
Fragments arranged by size:
3022 2681 1071 610 115
BstBI TT'CG_AA
Cuts at: 1620 5860 1620
Size: 4240 3259
BstEII G'GTnAC_C
Cuts at: 155 155
Size: 7499
BstXI CCAn_nnnn'nTGG
Cuts at: 783 2673 3379 3399 4455 783
Size: 1890 706 20 1056 3827
Fragments arranged by size:
3827 1890 1056 706 20
BstYI r'GATC_y
Cuts at: 1 4524 4991 5014 5349 5595 5901 5913 5999
Size: 4523 467 23 335 246 306 12 86
Cuts at: 5999 7051 1
Size: 1052 449
Fragments arranged by size:
4523 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 890 3659 890
Size: 2769 4730
BtgI C'CryG_G
Cuts at: 107 1307 1406 2598 3632 4017 4364 107
Size: 1200 99 1192 1034 385 347 3242
Fragments arranged by size:
3242 1200 1192 1034 385 347 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 939 1806 5449 5630 5793 939
Size: 867 3643 181 163 2645
Fragments arranged by size:
3643 2645 867 181 163
BtsI GCAGTG_nn'
Cuts at: 567 1295 3078 4189 4396 4742 567
Size: 728 1783 1111 207 346 3324
Fragments arranged by size:
3324 1783 1111 728 346 207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1243 1278 1243
Size: 35 7464
DraI TTT'AAA
Cuts at: 1737 4498 1737
Size: 2761 4738
DraIII CAC_nnn'GTG
Cuts at: 756 4266 756
Size: 3510 3989
DrdI GACnn_nn'nnGTC
Cuts at: 3358 5205 6549 3358
Size: 1847 1344 4308
Fragments arranged by size:
4308 1847 1344
EaeI y'GGCC_r
Cuts at: 939 986 1124 1310 1358 2564 2654 2663 3381
Size: 47 138 186 48 1206 90 9 718
Cuts at: 3381 3635 3728 4049 4331 5084 5258 5649 5676
Size: 254 93 321 282 753 174 391 27
Cuts at: 5676 7021 939
Size: 1345 1417
Fragments arranged by size:
1417 1345 1206 753 718 391 321 282
254 186 174 138 93 90 48 47
27 9
EagI C'GGCC_G
Cuts at: 939 5084 939
Size: 4145 3354
EarI CTCTTCn'nnn_
Cuts at: 664 823 1752 2204 4120 4469 5522 5732 664
Size: 159 929 452 1916 349 1053 210 2431
Fragments arranged by size:
2431 1916 1053 929 452 349 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1810 2638 3226 3634 3900 4414 5707 6449 6595
Size: 828 588 408 266 514 1293 742 146
Cuts at: 6595 7309 1810
Size: 714 2000
Fragments arranged by size:
2000 1293 828 742 714 588 514 408
266 146
EcoICRI GAG'CTC
Cuts at: 1236 2946 3293 4511 1236
Size: 1710 347 1218 4224
Fragments arranged by size:
4224 1710 1218 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 374 712 1204 1538 2422 2427 2704 2758
Size: 106 338 492 334 884 5 277 54
Cuts at: 2758 2860 2962 3022 3421 3703 3784 4219 4324
Size: 102 102 60 399 282 81 435 105
Cuts at: 4324 5324 5756 6109 6771 268
Size: 1000 432 353 662 996
Fragments arranged by size:
1000 996 884 662 492 435 432 399
353 338 334 282 277 106 105 102
102 81 60 54 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3244 3658 4051 4078 3244
Size: 414 393 27 6665
Fragments arranged by size:
6665 414 393 27
EcoO109I rG'GnC_Cy
Cuts at: 600 1215 1541 2603 2769 2865 2928 3396 3453
Size: 615 326 1062 166 96 63 468 57
Cuts at: 3453 3939 4161 4638 600
Size: 486 222 477 3461
Fragments arranged by size:
3461 1062 615 486 477 468 326 222
166 96 63 57
EcoRI G'AATT_C
Cuts at: 4503 4503
Size: 7499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 991 1083 5518 991
Size: 92 4435 2972
Fragments arranged by size:
4435 2972 92
FspI TGC'GCA
Cuts at: 1429 2809 3110 5280 1429
Size: 1380 301 2170 3648
Fragments arranged by size:
3648 2170 1380 301
HaeII r_GCGC'y
Cuts at: 2614 2721 2852 3781 4027 4933 5181 6411 7182
Size: 107 131 929 246 906 248 1230 771
Cuts at: 7182 2614
Size: 2931
Fragments arranged by size:
2931 1230 929 906 771 248 246 131
107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 687 719 1966 1998 2920 2952 3046 3078 3451
Size: 32 1247 32 922 32 94 32 373
Cuts at: 3451 3483 5341 5371 5373 5403 687
Size: 32 1858 30 2 30 2783
Fragments arranged by size:
2783 1858 1247 922 373 94 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4538 4538
Size: 7499
HindIII A'AGCT_T
Cuts at: 1740 1740
Size: 7499
KasI G'GCGC_C
Cuts at: 4929 5177 4929
Size: 248 7251
KpnI G_GTAC'C
Cuts at: 1136 4519 1136
Size: 3383 4116
MluI A'CGCG_T
Cuts at: 6932 6932
Size: 7499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 418 482 2867 2903 3521 6258 6442 418
Size: 64 2385 36 618 2737 184 1475
Fragments arranged by size:
2737 2385 1475 618 184 64 36
MscI TGG'CCA
Cuts at: 1312 2566 2665 3383 3637 3730 4051 4333 5260
Size: 1254 99 718 254 93 321 282 927
Cuts at: 5260 1312
Size: 3551
Fragments arranged by size:
3551 1254 927 718 321 282 254 99
93
MslI CAynn'nnrTG
Cuts at: 427 548 781 1306 1435 1445 2539 2825 5488
Size: 121 233 525 129 10 1094 286 2663
Cuts at: 5488 5615 427
Size: 127 2311
Fragments arranged by size:
2663 2311 1094 525 286 233 129 127
121 10
MspA1I CmG'CkG
Cuts at: 173 625 1129 1847 2553 2600 2673 2700 3491
Size: 452 504 718 706 47 73 27 791
Cuts at: 3491 3579 4101 4610 4924 5284 6068 6313 173
Size: 88 522 509 314 360 784 245 1359
Fragments arranged by size:
1359 791 784 718 706 522 509 504
452 360 314 245 88 73 47 27
NaeI GCC'GGC
Cuts at: 3753 5680 3753
Size: 1927 5572
NarI GG'CG_CC
Cuts at: 4930 5178 4930
Size: 248 7251
NcoI C'CATG_G
Cuts at: 107 107
Size: 7499
NgoMIV G'CCGG_C
Cuts at: 3751 5678 3751
Size: 1927 5572
NruI TCG'CGA
Cuts at: 3820 3820
Size: 7499
NsiI A_TGCA'T
Cuts at: 1577 1577
Size: 7499
NspI r_CATG'y
Cuts at: 657 1440 2538 2682 3708 4341 4552 5052 6655
Size: 783 1098 144 1026 633 211 500 1603
Cuts at: 6655 657
Size: 1501
Fragments arranged by size:
1603 1501 1098 1026 783 633 500 211
144
PasI CC'CwG_GG
Cuts at: 1807 1807
Size: 7499
PciI A'CATG_T
Cuts at: 653 4337 6651 653
Size: 3684 2314 1501
Fragments arranged by size:
3684 2314 1501
PflMI CCAn_nnn'nTGG
Cuts at: 1356 2556 1356
Size: 1200 6299
PfoI T'CCnGG_A
Cuts at: 1270 2371 4196 4301 1270
Size: 1101 1825 105 4468
Fragments arranged by size:
4468 1825 1101 105
PmeI GTTT'AAAC
Cuts at: 4498 4498
Size: 7499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1601 1633 5945 5977 274
Size: 32 1295 32 4312 32 1796
Fragments arranged by size:
4312 1796 1295 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 600 1541 2928 3453 4161 600
Size: 941 1387 525 708 3938
Fragments arranged by size:
3938 1387 941 708 525
PspOMI G'GGCC_C
Cuts at: 1214 2603 2864 3938 1214
Size: 1389 261 1074 4775
Fragments arranged by size:
4775 1389 1074 261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2725 2757 2725
Size: 32 7467
PstI C_TGCA'G
Cuts at: 1786 2860 3578 3872 4106 4546 5231 1786
Size: 1074 718 294 234 440 685 4054
Fragments arranged by size:
4054 1074 718 685 440 294 234
PvuI CG_AT'CG
Cuts at: 104 2796 104
Size: 2692 4807
PvuII CAG'CTG
Cuts at: 173 625 2673 2700 3579 4101 4924 5284 173
Size: 452 2048 27 879 522 823 360 2388
Fragments arranged by size:
2388 2048 879 823 522 452 360 27
RsrII CG'GwC_CG
Cuts at: 5694 5694
Size: 7499
SacI G_AGCT'C
Cuts at: 1238 2948 3295 4513 1238
Size: 1710 347 1218 4224
Fragments arranged by size:
4224 1710 1218 347
SacII CC_GC'GG
Cuts at: 2601 2601
Size: 7499
SalI G'TCGA_C
Cuts at: 4536 4536
Size: 7499
SapI GCTCTTCn'nnn_
Cuts at: 664 2204 5522 5732 664
Size: 1540 3318 210 2431
Fragments arranged by size:
3318 2431 1540 210
SbfI CC_TGCA'GG
Cuts at: 4546 4546
Size: 7499
ScaI AGT'ACT
Cuts at: 7016 7016
Size: 7499
SexAI A'CCwGG_T
Cuts at: 602 602
Size: 7499
SfcI C'TryA_G
Cuts at: 33 1776 1782 2856 3436 3574 3868 4102 4293
Size: 1743 6 1074 580 138 294 234 191
Cuts at: 4293 4542 5227 6195 6386 33
Size: 249 685 968 191 1146
Fragments arranged by size:
1743 1146 1074 968 685 580 294 249
234 191 191 138 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2653 2662 2653
Size: 9 7490
SfoI GGC'GCC
Cuts at: 4931 5179 4931
Size: 248 7251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7499
SmaI CCC'GGG
Cuts at: 1191 2425 3052 3211 4521 1191
Size: 1234 627 159 1310 4169
Fragments arranged by size:
4169 1310 1234 627 159
SmlI C'TyrA_G
Cuts at: 4654 5569 6283 6545 4654
Size: 915 714 262 5608
Fragments arranged by size:
5608 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7499
SphI G_CATG'C
Cuts at: 2682 3708 4552 5052 2682
Size: 1026 844 500 5129
Fragments arranged by size:
5129 1026 844 500
StuI AGG'CCT
Cuts at: 3220 4036 3220
Size: 816 6683
StyI C'CwwG_G
Cuts at: 107 1239 1463 2567 3392 3638 3929 4052 4058
Size: 1132 224 1104 825 246 291 123 6
Cuts at: 4058 4166 4328 4633 4987 107
Size: 108 162 305 354 2619
Fragments arranged by size:
2619 1132 1104 825 354 305 291 246
224 162 123 108 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2333 3433 4045 5082 5215 5674 5883 2333
Size: 1100 612 1037 133 459 209 3949
Fragments arranged by size:
3949 1100 1037 612 459 209 133
TatI w'GTAC_w
Cuts at: 875 2702 3740 7014 875
Size: 1827 1038 3274 1360
Fragments arranged by size:
3274 1827 1360 1038
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 432 464 870 902 1481 1513 2329 2361 4394
Size: 32 406 32 579 32 816 32 2033
Cuts at: 4394 4426 7016 7048 432
Size: 32 2590 32 883
Fragments arranged by size:
2590 2033 883 816 579 406 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1980 5296 1980
Size: 3316 4183
XbaI T'CTAG_A
Cuts at: 4530 4530
Size: 7499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2466 2574 4071 4994 2466
Size: 108 1497 923 4971
Fragments arranged by size:
4971 1497 923 108
XmaI C'CCGG_G
Cuts at: 1189 2423 3050 3209 4519 1189
Size: 1234 627 159 1310 4169
Fragments arranged by size:
4169 1310 1234 627 159
XmnI GAAnn'nnTTC
Cuts at: 2059 2737 4129 2059
Size: 678 1392 5429
Fragments arranged by size:
5429 1392 678
ZraI GAC'GTC
Cuts at: 3467 4239 3467
Size: 772 6727
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AloI AscI AvrII BmtI BsiWI BstZ17I
ClaI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI PacI PmlI PshAI PsiI PspXI SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI