Restriction map for
pF1KA0586
(Circular) (Six-base) MAPSORT of: pF1KA0586.seq Check: 4332 from: 1 to: 7712
pF1KA0586 7712 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 731 2358 3276 731
Size: 1627 918 5167
Fragments arranged by size:
5167 1627 918
AccI GT'mk_AC
Cuts at: 427 4750 427
Size: 4323 3389
Acc65I G'GTAC_C
Cuts at: 4728 4728
Size: 7712
AclI AA'CG_TT
Cuts at: 7552 7552
Size: 7712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3818 4391 5537 5969 6322 6984 3818
Size: 573 1146 432 353 662 4546
Fragments arranged by size:
4546 1146 662 573 432 353
AflIII A'CryG_T
Cuts at: 729 4449 4543 5064 6864 7145 729
Size: 3720 94 521 1800 281 1296
Fragments arranged by size:
3720 1800 1296 521 281 94
AhdI GACnn_n'nnGTC
Cuts at: 4683 4683
Size: 7712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2963 3231 2963
Size: 268 7444
AlwNI CAG_nnn'CTG
Cuts at: 610 2728 3724 4653 5017 6455 610
Size: 2118 996 929 364 1438 1867
Fragments arranged by size:
2118 1867 1438 996 929 364
ApaLI G'TGCA_C
Cuts at: 3216 6550 3216
Size: 3334 4378
ApoI r'AATT_y
Cuts at: 563 1214 1470 1712 2717 3970 4299 4716 7065
Size: 651 256 242 1005 1253 329 417 2349
Cuts at: 7065 7321 563
Size: 256 954
Fragments arranged by size:
2349 1253 1005 954 651 417 329 256
256 242
AseI AT'TA_AT
Cuts at: 20 20
Size: 7712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7712
AvaI C'yCGr_G
Cuts at: 4633 4732 4633
Size: 99 7613
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 499 532 4691 4724 499
Size: 33 4159 33 3487
Fragments arranged by size:
4159 3487 33 33
BamHI G'GATC_C
Cuts at: 2481 4737 2481
Size: 2256 5456
BanI G'GyrC_C
Cuts at: 3025 3688 4728 5142 5390 5425 3025
Size: 663 1040 414 248 35 5312
Fragments arranged by size:
5312 1040 663 414 248 35
BanII G_rGCy'C
Cuts at: 2927 4726 5756 2927
Size: 1799 1030 4883
Fragments arranged by size:
4883 1799 1030
BbeI G_GCGC'C
Cuts at: 3029 5146 5394 3029
Size: 2117 248 5347
Fragments arranged by size:
5347 2117 248
BbsI GAAGACnn'nnnn_
Cuts at: 211 4679 211
Size: 4468 3244
BbvCI CC'TCA_GC
Cuts at: 2489 2489
Size: 7712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3082 3116 90
Size: 34 2958 34 4686
Fragments arranged by size:
4686 2958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5610 6666 7712 5610
Size: 1056 1046 5610
Fragments arranged by size:
5610 1056 1046
BclI T'GATC_A
Cuts at: 786 1758 5232 786
Size: 972 3474 3266
Fragments arranged by size:
3474 3266 972
BglII A'GATC_T
Cuts at: 1458 1504 5227 7264 1458
Size: 46 3723 2037 1906
Fragments arranged by size:
3723 2037 1906 46
BlpI GC'TnA_GC
Cuts at: 3641 4824 3641
Size: 1183 6529
Bme1580I G_kGCm'C
Cuts at: 3220 5337 5430 6554 3220
Size: 2117 93 1124 4378
Fragments arranged by size:
4378 2117 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 566 2507 4377 4924 5005 5340 7484 566
Size: 1941 1870 547 81 335 2144 794
Fragments arranged by size:
2144 1941 1870 794 547 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4659 6079 4659
Size: 1420 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 2375 2450 2603 2761 3016 3281 3416 3638
Size: 1460 75 153 158 255 265 135 222
Cuts at: 3638 3760 915
Size: 122 4867
Fragments arranged by size:
4867 1460 265 255 222 158 153 135
122 75
Bpu10I CC'TnA_GC
Cuts at: 2489 3272 3605 2489
Size: 783 333 6596
Fragments arranged by size:
6596 783 333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 3548 4888 5767 6481 6779 877
Size: 2671 1340 879 714 298 1810
Fragments arranged by size:
2671 1810 1340 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2088 3100 3521 7416 2088
Size: 1012 421 3895 2384
Fragments arranged by size:
3895 2384 1012 421
BsaAI yAC'GTr
Cuts at: 259 4450 5065 259
Size: 4191 615 2906
Fragments arranged by size:
4191 2906 615
BsaBI GATnn'nnATC
Cuts at: 6 3320 4508 5226 7263 6
Size: 3314 1188 718 2037 455
Fragments arranged by size:
3314 2037 1188 718 455
BsaHI Gr'CG_yC
Cuts at: 3026 5143 5391 6093 7589 3026
Size: 2117 248 702 1496 3149
Fragments arranged by size:
3149 2117 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4901 5422 6100 6511 6658 6932 4901
Size: 521 678 411 147 274 5681
Fragments arranged by size:
5681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 618 648 2348 2378 3411 3414 3441 3444 618
Size: 30 1700 30 1033 3 27 3 4886
Fragments arranged by size:
4886 1700 1033 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 918 1553 2477 2579 2582 3419 918
Size: 635 924 102 3 837 5211
Fragments arranged by size:
5211 924 837 635 102 3
BseYI C'CCAG_C
Cuts at: 3123 4028 4097 5137 5895 6560 6968 3123
Size: 905 69 1040 758 665 408 3867
Fragments arranged by size:
3867 1040 905 758 665 408 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 872 2521 670
Size: 202 1649 5861
Fragments arranged by size:
5861 1649 202
BsiEI CG_ry'CG
Cuts at: 104 5254 5300 6530 104
Size: 5150 46 1230 1286
Fragments arranged by size:
5150 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3220 3748 4726 5504 5694 6554 3220
Size: 528 978 778 190 860 4378
Fragments arranged by size:
4378 978 860 778 528 190
BsmI GAATG_Cn'
Cuts at: 752 752
Size: 7712
Bsp1286I G_dGCh'C
Cuts at: 2927 3220 3748 4726 5337 5430 5504 5694 5756
Size: 293 528 978 611 93 74 190 62
Cuts at: 5756 6554 2927
Size: 798 4085
Fragments arranged by size:
4085 978 798 611 528 293 190 93
74 62
BspHI T'CATG_A
Cuts at: 783 1380 3324 6144 783
Size: 597 1944 2820 2351
Fragments arranged by size:
2820 2351 1944 597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 731 2358 2493 3276 3539 4762 4976 5278 5659
Size: 1627 135 783 263 1223 214 302 381
Cuts at: 5659 731
Size: 2784
Fragments arranged by size:
2784 1627 1223 783 381 302 263 214
135
BsrBI CCG'CTC
Cuts at: 50 6004 6058 7542 7707 50
Size: 5954 54 1484 165 55
Fragments arranged by size:
5954 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 620 3110 5624 620
Size: 2490 2514 2708
Fragments arranged by size:
2708 2514 2490
BsrFI r'CCGG_y
Cuts at: 3204 3685 5710 5891 3204
Size: 481 2025 181 5025
Fragments arranged by size:
5025 2025 481 181
BssSI C'ACGA_G
Cuts at: 5983 6691 5983
Size: 708 7004
BstAPI GCAn_nnn'nTGC
Cuts at: 205 363 610 613 5089 205
Size: 158 247 3 4476 2828
Fragments arranged by size:
4476 2828 247 158 3
BstBI TT'CG_AA
Cuts at: 6073 6073
Size: 7712
BstXI CCAn_nnnn'nTGG
Cuts at: 939 939
Size: 7712
BstYI r'GATC_y
Cuts at: 1 1458 1504 2481 4737 5204 5227 5562 5808
Size: 1457 46 977 2256 467 23 335 246
Cuts at: 5808 6114 6126 6212 7264 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2256 1457 1052 977 467 449 335 306
246 86 46 23 12
BstZ17I GTA'TAC
Cuts at: 428 428
Size: 7712
BtgI C'CryG_G
Cuts at: 317 596 3514 317
Size: 279 2918 4515
Fragments arranged by size:
4515 2918 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5662 5843 6006 5662
Size: 181 163 7368
Fragments arranged by size:
7368 181 163
BtsI GCAGTG_nn'
Cuts at: 359 944 2822 3027 4574 4955 359
Size: 585 1878 205 1547 381 3116
Fragments arranged by size:
3116 1878 1547 585 381 205
ClaI AT'CG_AT
Cuts at: 2684 2684
Size: 7712
DraI TTT'AAA
Cuts at: 464 1031 4711 464
Size: 567 3680 3465
Fragments arranged by size:
3680 3465 567
DraIII CAC_nnn'GTG
Cuts at: 574 4501 574
Size: 3927 3785
DrdI GACnn_nn'nnGTC
Cuts at: 5418 6762 5418
Size: 1344 6368
EaeI y'GGCC_r
Cuts at: 1389 5297 5471 5862 5889 7234 1389
Size: 3908 174 391 27 1345 1867
Fragments arranged by size:
3908 1867 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5297 5297
Size: 7712
EarI CTCTTCn'nnn_
Cuts at: 112 377 1325 1874 2639 3539 5735 5945 112
Size: 265 948 549 765 900 2196 210 1879
Fragments arranged by size:
2196 1879 948 900 765 549 265 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2786 5920 6662 6808 7522 2786
Size: 3134 742 146 714 2976
Fragments arranged by size:
3134 2976 742 714 146
EcoICRI GAG'CTC
Cuts at: 4724 4724
Size: 7712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 2375 2450 2603 2761 3016 3281 3416 3638
Size: 1460 75 153 158 255 265 135 222
Cuts at: 3638 3760 3818 4391 5537 5969 6322 6984 915
Size: 122 58 573 1146 432 353 662 1643
Fragments arranged by size:
1643 1460 1146 662 573 432 353 265
255 222 158 153 135 122 75 58
EcoNI CCTnn'n_nnAGG
Cuts at: 3938 3938
Size: 7712
EcoO109I rG'GnC_Cy
Cuts at: 3151 3696 4851 3151
Size: 545 1155 6012
Fragments arranged by size:
6012 1155 545
EcoRI G'AATT_C
Cuts at: 1470 4716 1470
Size: 3246 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3589 5731 3589
Size: 2142 5570
FspI TGC'GCA
Cuts at: 5493 5493
Size: 7712
HaeII r_GCGC'y
Cuts at: 3029 5146 5394 6624 7395 3029
Size: 2117 248 1230 771 3346
Fragments arranged by size:
3346 2117 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1117 1131 1149 1163 1711 1743 2039 2071 2348
Size: 14 18 14 548 32 296 32 277
Cuts at: 2348 2380 2601 2633 3874 3906 4124 4156 4669
Size: 32 221 32 1241 32 218 32 513
Cuts at: 4669 4701 5554 5584 5586 5616 1117
Size: 32 853 30 2 30 3213
Fragments arranged by size:
3213 1241 853 548 513 296 277 221
218 32 32 32 32 32 32 32
30 30 18 14 14 2
HincII GTy'rAC
Cuts at: 1836 3090 4751 1836
Size: 1254 1661 4797
Fragments arranged by size:
4797 1661 1254
HindIII A'AGCT_T
Cuts at: 1450 1480 1450
Size: 30 7682
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 7712
KasI G'GCGC_C
Cuts at: 3025 5142 5390 3025
Size: 2117 248 5347
Fragments arranged by size:
5347 2117 248
KpnI G_GTAC'C
Cuts at: 4732 4732
Size: 7712
MluI A'CGCG_T
Cuts at: 7145 7145
Size: 7712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 207 3427 4271 6471 6655 207
Size: 3220 844 2200 184 1264
Fragments arranged by size:
3220 2200 1264 844 184
MscI TGG'CCA
Cuts at: 1391 5473 1391
Size: 4082 3630
MslI CAynn'nnrTG
Cuts at: 112 782 3980 4126 5701 5828 112
Size: 670 3198 146 1575 127 1996
Fragments arranged by size:
3198 1996 1575 670 146 127
MspA1I CmG'CkG
Cuts at: 3436 4108 4369 4823 5137 5497 6281 6526 3436
Size: 672 261 454 314 360 784 245 4622
Fragments arranged by size:
4622 784 672 454 360 314 261 245
NaeI GCC'GGC
Cuts at: 3206 3687 5893 3206
Size: 481 2206 5025
Fragments arranged by size:
5025 2206 481
NarI GG'CG_CC
Cuts at: 3026 5143 5391 3026
Size: 2117 248 5347
Fragments arranged by size:
5347 2117 248
NcoI C'CATG_G
Cuts at: 317 3514 317
Size: 3197 4515
NgoMIV G'CCGG_C
Cuts at: 3204 3685 5891 3204
Size: 481 2206 5025
Fragments arranged by size:
5025 2206 481
NspI r_CATG'y
Cuts at: 486 1914 4547 4765 5265 6868 486
Size: 1428 2633 218 500 1603 1330
Fragments arranged by size:
2633 1603 1428 1330 500 218
PciI A'CATG_T
Cuts at: 4543 6864 4543
Size: 2321 5391
PflMI CCAn_nnn'nTGG
Cuts at: 2353 3515 2353
Size: 1162 6550
PfoI T'CCnGG_A
Cuts at: 2658 2658
Size: 7712
PmeI GTTT'AAAC
Cuts at: 4711 4711
Size: 7712
PmlI CAC'GTG
Cuts at: 259 259
Size: 7712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3541 3573 6158 6190 3541
Size: 32 2585 32 5063
Fragments arranged by size:
5063 2585 32 32
PpuMI rG'GwC_Cy
Cuts at: 3151 3151
Size: 7712
PspXI vC'TCGA_Gb
Cuts at: 4633 4633
Size: 7712
PstI C_TGCA'G
Cuts at: 606 1710 4759 5444 606
Size: 1104 3049 685 2874
Fragments arranged by size:
3049 2874 1104 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7712
PvuII CAG'CTG
Cuts at: 5137 5497 5137
Size: 360 7352
RsrII CG'GwC_CG
Cuts at: 5907 5907
Size: 7712
SacI G_AGCT'C
Cuts at: 4726 4726
Size: 7712
SalI G'TCGA_C
Cuts at: 4749 4749
Size: 7712
SapI GCTCTTCn'nnn_
Cuts at: 1325 1874 5735 5945 1325
Size: 549 3861 210 3092
Fragments arranged by size:
3861 3092 549 210
SbfI CC_TGCA'GG
Cuts at: 4759 4759
Size: 7712
ScaI AGT'ACT
Cuts at: 1562 7229 1562
Size: 5667 2045
SexAI A'CCwGG_T
Cuts at: 4497 4497
Size: 7712
SfcI C'TryA_G
Cuts at: 33 602 1706 3161 4755 5440 6408 6599 33
Size: 569 1104 1455 1594 685 968 191 1146
Fragments arranged by size:
1594 1455 1146 1104 968 685 569 191
SfoI GGC'GCC
Cuts at: 3027 5144 5392 3027
Size: 2117 248 5347
Fragments arranged by size:
5347 2117 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7712
SmaI CCC'GGG
Cuts at: 4734 4734
Size: 7712
SmlI C'TyrA_G
Cuts at: 892 3563 4633 4867 5782 6496 6758 892
Size: 2671 1070 234 915 714 262 1846
Fragments arranged by size:
2671 1846 1070 915 714 262 234
SpeI A'CTAG_T
Cuts at: 67 238 898 67
Size: 171 660 6881
Fragments arranged by size:
6881 660 171
SphI G_CATG'C
Cuts at: 486 1914 4765 5265 486
Size: 1428 2851 500 2933
Fragments arranged by size:
2933 2851 1428 500
SspI AAT'ATT
Cuts at: 2236 2236
Size: 7712
StyI C'CwwG_G
Cuts at: 317 1386 3514 3692 4846 5200 317
Size: 1069 2128 178 1154 354 2829
Fragments arranged by size:
2829 2128 1154 1069 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 585 3115 4060 5295 5428 5887 6096 585
Size: 2530 945 1235 133 459 209 2201
Fragments arranged by size:
2530 2201 1235 945 459 209 133
TatI w'GTAC_w
Cuts at: 1542 1560 7227 1542
Size: 18 5667 2027
Fragments arranged by size:
5667 2027 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7229 7261 7229
Size: 32 7680
Tth111I GACn'n_nGTC
Cuts at: 5509 5509
Size: 7712
XbaI T'CTAG_A
Cuts at: 4743 4743
Size: 7712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5207 5207
Size: 7712
XhoI C'TCGA_G
Cuts at: 4633 4633
Size: 7712
XmaI C'CCGG_G
Cuts at: 4732 4732
Size: 7712
XmnI GAAnn'nnTTC
Cuts at: 582 1860 2735 3572 582
Size: 1278 875 837 4722
Fragments arranged by size:
4722 1278 875 837
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AloI ApaI AscI
AvrII BfrBI BglI BmgBI BmtI BsiWI BsmBI BspEI
BsrGI BssHII BstEII Bsu36I CspCI EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PshAI PsiI PspOMI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI