Restriction map for
pF1KA0581
(Circular) (Six-base) MAPSORT of: pF1KA0581.seq Check: 1788 from: 1 to: 6761
pF1KA0581 6761 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2787 3525 2787
Size: 738 6023
AccI GT'mk_AC
Cuts at: 2480 3799 2480
Size: 1319 5442
Acc65I G'GTAC_C
Cuts at: 3777 3777
Size: 6761
AclI AA'CG_TT
Cuts at: 6601 6601
Size: 6761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 4586 5018 5765 6033 2126
Size: 2460 432 747 268 2854
Fragments arranged by size:
2854 2460 747 432 268
AflII C'TTAA_G
Cuts at: 583 859 583
Size: 276 6485
AflIII A'CryG_T
Cuts at: 542 1058 1912 1914 4113 5217 6194 542
Size: 516 854 2 2199 1104 977 1109
Fragments arranged by size:
2199 1109 1104 977 854 516 2
AgeI A'CCGG_T
Cuts at: 5149 5149
Size: 6761
AleI CACnn'nnGTG
Cuts at: 421 421
Size: 6761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2606 2606
Size: 6761
AlwNI CAG_nnn'CTG
Cuts at: 2457 2767 3153 3513 3676 4066 5633 2457
Size: 310 386 360 163 390 1567 3585
Fragments arranged by size:
3585 1567 390 386 360 310 163
ApaLI G'TGCA_C
Cuts at: 130 5531 130
Size: 5401 1360
ApoI r'AATT_y
Cuts at: 187 799 1490 1775 1861 2371 2969 3565 3595
Size: 612 691 285 86 510 598 596 30
Cuts at: 3595 3739 3765 6114 6370 187
Size: 144 26 2349 256 578
Fragments arranged by size:
2349 691 612 598 596 578 510 285
256 144 86 30 26
AseI AT'TA_AT
Cuts at: 20 1088 20
Size: 1068 5693
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6761
AvaI C'yCGr_G
Cuts at: 3583 3781 3583
Size: 198 6563
BamHI G'GATC_C
Cuts at: 3786 3786
Size: 6761
BanI G'GyrC_C
Cuts at: 181 395 2775 3777 4191 4439 4474 181
Size: 214 2380 1002 414 248 35 2468
Fragments arranged by size:
2468 2380 1002 414 248 214 35
BanII G_rGCy'C
Cuts at: 120 1427 1604 3775 4805 120
Size: 1307 177 2171 1030 2076
Fragments arranged by size:
2171 2076 1307 1030 177
BbeI G_GCGC'C
Cuts at: 399 4195 4443 399
Size: 3796 248 2717
Fragments arranged by size:
3796 2717 248
BbsI GAAGACnn'nnnn_
Cuts at: 679 1061 1802 3397 3638 679
Size: 382 741 1595 241 3802
Fragments arranged by size:
3802 1595 741 382 241
BbvCI CC'TCA_GC
Cuts at: 1940 1940
Size: 6761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 171 205 2389 2423 171
Size: 34 2184 34 4509
Fragments arranged by size:
4509 2184 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2220 4659 5420 6761 2220
Size: 2439 761 1341 2220
Fragments arranged by size:
2439 2220 1341 761
BclI T'GATC_A
Cuts at: 413 834 3057 4281 413
Size: 421 2223 1224 2893
Fragments arranged by size:
2893 2223 1224 421
BfrBI ATG'CAT
Cuts at: 556 556
Size: 6761
BglI GCCn_nnn'nGGC
Cuts at: 1378 1378
Size: 6761
BglII A'GATC_T
Cuts at: 2793 4276 6313 2793
Size: 1483 2037 3241
Fragments arranged by size:
3241 2037 1483
BlpI GC'TnA_GC
Cuts at: 1888 2579 3873 1888
Size: 691 1294 4776
Fragments arranged by size:
4776 1294 691
Bme1580I G_kGCm'C
Cuts at: 134 184 4386 4479 5535 134
Size: 50 4202 93 1056 1360
Fragments arranged by size:
4202 1360 1056 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 3973 4054 4389 6533 3973
Size: 81 335 2144 4201
Fragments arranged by size:
4201 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 769 2696 5128 769
Size: 1927 2432 2402
Fragments arranged by size:
2432 2402 1927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 584 716 1472 1572 1838 2660 2666 2744
Size: 163 132 756 100 266 822 6 78
Cuts at: 2744 3565 3687 421
Size: 821 122 3495
Fragments arranged by size:
3495 822 821 756 266 163 132 122
100 78 6
Bpu10I CC'TnA_GC
Cuts at: 1940 1940
Size: 6761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 157 701 3006 3937 4816 5308 5606 130
Size: 27 544 2305 931 879 492 298 1285
Fragments arranged by size:
2305 1285 931 879 544 492 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 6465 6465
Size: 6761
BsaAI yAC'GTr
Cuts at: 367 1915 2196 4114 367
Size: 1548 281 1918 3014
Fragments arranged by size:
3014 1918 1548 281
BsaBI GATnn'nnATC
Cuts at: 6 4275 6312 6
Size: 4269 2037 455
Fragments arranged by size:
4269 2037 455
BsaHI Gr'CG_yC
Cuts at: 396 4192 4440 5142 6638 396
Size: 3796 248 702 1496 519
Fragments arranged by size:
3796 1496 702 519 248
BsaWI w'CCGG_w
Cuts at: 3404 3950 4471 5149 5423 5570 5981 3404
Size: 546 521 678 274 147 411 4184
Fragments arranged by size:
4184 678 546 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1567 1597 1836 1866 1567
Size: 30 239 30 6462
Fragments arranged by size:
6462 239 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1128 1575 2088 3400 3724 3734 3740 1128
Size: 447 513 1312 324 10 6 4149
Fragments arranged by size:
4149 1312 513 447 324 10 6
BseYI C'CCAG_C
Cuts at: 3046 3714 4186 4944 5521 6017 3046
Size: 668 472 758 577 496 3790
Fragments arranged by size:
3790 758 668 577 496 472
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3448 3622 3448
Size: 174 6587
BsiEI CG_ry'CG
Cuts at: 104 4303 4349 5557 104
Size: 4199 46 1208 1308
Fragments arranged by size:
4199 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 134 1166 3775 4553 4743 5535 134
Size: 1032 2609 778 190 792 1360
Fragments arranged by size:
2609 1360 1032 792 778 190
BsmI GAATG_Cn'
Cuts at: 1959 1959
Size: 6761
Bsp1286I G_dGCh'C
Cuts at: 120 134 184 1166 1427 1604 3775 4386 4479
Size: 14 50 982 261 177 2171 611 93
Cuts at: 4479 4553 4743 4805 5535 120
Size: 74 190 62 730 1346
Fragments arranged by size:
2171 1346 982 730 611 261 190 177
93 74 62 50 14
BspEI T'CCGG_A
Cuts at: 5981 5981
Size: 6761
BspHI T'CATG_A
Cuts at: 2069 5937 2069
Size: 3868 2893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 536 1948 2787 3525 3811 4025 4327 4708 536
Size: 1412 839 738 286 214 302 381 2589
Fragments arranged by size:
2589 1412 839 738 381 302 286 214
BsrBI CCG'CTC
Cuts at: 50 5053 5107 6591 6756 50
Size: 5003 54 1484 165 55
Fragments arranged by size:
5003 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4673 4673
Size: 6761
BsrFI r'CCGG_y
Cuts at: 4759 4940 5149 4759
Size: 181 209 6371
Fragments arranged by size:
6371 209 181
BsrGI T'GTAC_A
Cuts at: 740 740
Size: 6761
BssSI C'ACGA_G
Cuts at: 439 463 5032 5390 439
Size: 24 4569 358 1810
Fragments arranged by size:
4569 1810 358 24
BstAPI GCAn_nnn'nTGC
Cuts at: 138 3153 4138 138
Size: 3015 985 2761
Fragments arranged by size:
3015 2761 985
BstBI TT'CG_AA
Cuts at: 5122 5122
Size: 6761
BstXI CCAn_nnnn'nTGG
Cuts at: 1129 1129
Size: 6761
BstYI r'GATC_y
Cuts at: 1 300 870 2793 3509 3786 4253 4276 4611
Size: 299 570 1923 716 277 467 23 335
Cuts at: 4611 4857 5869 5955 5967 6313 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1923 1012 716 570 467 449 346 335
299 277 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 248 248
Size: 6761
BtgI C'CryG_G
Cuts at: 107 1239 107
Size: 1132 5629
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4711 4892 5055 4711
Size: 181 163 6417
Fragments arranged by size:
6417 181 163
BtsI GCAGTG_nn'
Cuts at: 4004 4004
Size: 6761
DraI TTT'AAA
Cuts at: 3760 3760
Size: 6761
DrdI GACnn_nn'nnGTC
Cuts at: 4467 5325 4467
Size: 858 5903
EaeI y'GGCC_r
Cuts at: 110 485 1119 1712 2379 2410 4346 4520 4911
Size: 375 634 593 667 31 1936 174 391
Cuts at: 4911 4938 6283 110
Size: 27 1345 588
Fragments arranged by size:
1936 1345 667 634 593 588 391 375
174 31 27
EagI C'GGCC_G
Cuts at: 4346 4346
Size: 6761
EarI CTCTTCn'nnn_
Cuts at: 1214 1466 1951 2035 3242 3377 4784 4994 1214
Size: 252 485 84 1207 135 1407 210 2981
Fragments arranged by size:
2981 1407 1207 485 252 210 135 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1396 3003 4969 5279 5425 6571 1396
Size: 1607 1966 310 146 1146 1586
Fragments arranged by size:
1966 1607 1586 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3773 3773
Size: 6761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 584 716 1472 1572 1838 2126 2660 2666
Size: 163 132 756 100 266 288 534 6
Cuts at: 2666 2744 3565 3687 4586 5018 5765 6033 421
Size: 78 821 122 899 432 747 268 1149
Fragments arranged by size:
1149 899 821 756 747 534 432 288
268 266 163 132 122 100 78 6
EcoNI CCTnn'n_nnAGG
Cuts at: 175 175
Size: 6761
EcoO109I rG'GnC_Cy
Cuts at: 354 3900 354
Size: 3546 3215
EcoRI G'AATT_C
Cuts at: 3595 3765 3595
Size: 170 6591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4780 4780
Size: 6761
FspI TGC'GCA
Cuts at: 4542 4542
Size: 6761
HaeII r_GCGC'y
Cuts at: 399 4195 4443 5465 6444 399
Size: 3796 248 1022 979 716
Fragments arranged by size:
3796 1022 979 716 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 231 263 1591 1623 3566 3598 3710
Size: 32 6 32 1328 32 1943 32 112
Cuts at: 3710 3742 4603 4633 4635 4665 193
Size: 32 861 30 2 30 2289
Fragments arranged by size:
2289 1943 1328 861 112 32 32 32
32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 3233 3800 3233
Size: 567 6194
HindIII A'AGCT_T
Cuts at: 2531 2531
Size: 6761
KasI G'GCGC_C
Cuts at: 395 4191 4439 395
Size: 3796 248 2717
Fragments arranged by size:
3796 2717 248
KpnI G_GTAC'C
Cuts at: 3781 3781
Size: 6761
MfeI C'AATT_G
Cuts at: 1289 2584 1289
Size: 1295 5466
MluI A'CGCG_T
Cuts at: 6194 6194
Size: 6761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 1592 1952 5432 5616 188
Size: 1404 360 3480 184 1333
Fragments arranged by size:
3480 1404 1333 360 184
MscI TGG'CCA
Cuts at: 487 1121 1714 2381 4522 487
Size: 634 593 667 2141 2726
Fragments arranged by size:
2726 2141 667 634 593
MslI CAynn'nnrTG
Cuts at: 421 2488 4750 4877 421
Size: 2067 2262 127 2305
Fragments arranged by size:
2305 2262 2067 127
MspA1I CmG'CkG
Cuts at: 1117 3153 3872 4186 4546 5559 5804 1117
Size: 2036 719 314 360 1013 245 2074
Fragments arranged by size:
2074 2036 1013 719 360 314 245
NaeI GCC'GGC
Cuts at: 4942 4942
Size: 6761
NarI GG'CG_CC
Cuts at: 396 4192 4440 396
Size: 3796 248 2717
Fragments arranged by size:
3796 2717 248
NcoI C'CATG_G
Cuts at: 107 1239 107
Size: 1132 5629
NdeI CA'TA_TG
Cuts at: 2514 2514
Size: 6761
NgoMIV G'CCGG_C
Cuts at: 4940 4940
Size: 6761
NsiI A_TGCA'T
Cuts at: 558 558
Size: 6761
NspI r_CATG'y
Cuts at: 546 1062 1596 3814 4314 5221 546
Size: 516 534 2218 500 907 2086
Fragments arranged by size:
2218 2086 907 534 516 500
PciI A'CATG_T
Cuts at: 542 1058 5217 542
Size: 516 4159 2086
Fragments arranged by size:
4159 2086 516
PflMI CCAn_nnn'nTGG
Cuts at: 494 1992 3423 494
Size: 1498 1431 3832
Fragments arranged by size:
3832 1498 1431
PfoI T'CCnGG_A
Cuts at: 2637 3416 3512 2637
Size: 779 96 5886
Fragments arranged by size:
5886 779 96
PmeI GTTT'AAAC
Cuts at: 3760 3760
Size: 6761
PmlI CAC'GTG
Cuts at: 1915 1915
Size: 6761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5900 5932 5900
Size: 32 6729
PpuMI rG'GwC_Cy
Cuts at: 354 354
Size: 6761
PshAI GACnn'nnGTC
Cuts at: 3238 3371 3238
Size: 133 6628
PstI C_TGCA'G
Cuts at: 1218 3536 3808 4493 1218
Size: 2318 272 685 3486
Fragments arranged by size:
3486 2318 685 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6761
PvuII CAG'CTG
Cuts at: 1117 3153 4186 4546 1117
Size: 2036 1033 360 3332
Fragments arranged by size:
3332 2036 1033 360
RsrII CG'GwC_CG
Cuts at: 4956 4956
Size: 6761
SacI G_AGCT'C
Cuts at: 3775 3775
Size: 6761
SalI G'TCGA_C
Cuts at: 3798 3798
Size: 6761
SapI GCTCTTCn'nnn_
Cuts at: 1951 4784 4994 1951
Size: 2833 210 3718
Fragments arranged by size:
3718 2833 210
SbfI CC_TGCA'GG
Cuts at: 3808 3808
Size: 6761
ScaI AGT'ACT
Cuts at: 1387 6278 1387
Size: 4891 1870
SexAI A'CCwGG_T
Cuts at: 2903 2903
Size: 6761
SfcI C'TryA_G
Cuts at: 33 1214 1575 2049 3532 3804 4489 5482 5673
Size: 1181 361 474 1483 272 685 993 191
Cuts at: 5673 33
Size: 1121
Fragments arranged by size:
1483 1181 1121 993 685 474 361 272
191
SfoI GGC'GCC
Cuts at: 397 4193 4441 397
Size: 3796 248 2717
Fragments arranged by size:
3796 2717 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6761
SmaI CCC'GGG
Cuts at: 3783 3783
Size: 6761
SmlI C'TyrA_G
Cuts at: 145 172 583 716 859 2985 3583 3916 4831
Size: 27 411 133 143 2126 598 333 915
Cuts at: 4831 5323 5585 145
Size: 492 262 1321
Fragments arranged by size:
2126 1321 915 598 492 411 333 262
143 133 27
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6761
SphI G_CATG'C
Cuts at: 3814 4314 3814
Size: 500 6261
StuI AGG'CCT
Cuts at: 2079 2079
Size: 6761
StyI C'CwwG_G
Cuts at: 107 350 488 1239 2262 3895 4249 107
Size: 243 138 751 1023 1633 354 2619
Fragments arranged by size:
2619 1633 1023 751 354 243 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1252 2736 4344 4477 4936 5145 1252
Size: 1484 1608 133 459 209 2868
Fragments arranged by size:
2868 1608 1484 459 209 133
TatI w'GTAC_w
Cuts at: 740 1385 6276 740
Size: 645 4891 1225
Fragments arranged by size:
4891 1225 645
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1836 1868 1901 1933 1967 1999 2258 2290 6278
Size: 32 33 32 34 32 259 32 3988
Cuts at: 6278 6310 1836
Size: 32 2287
Fragments arranged by size:
3988 2287 259 34 33 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4558 4558
Size: 6761
XbaI T'CTAG_A
Cuts at: 3792 3792
Size: 6761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1854 3534 3711 4256 1854
Size: 1680 177 545 4359
Fragments arranged by size:
4359 1680 545 177
XhoI C'TCGA_G
Cuts at: 3583 3583
Size: 6761
XmaI C'CCGG_G
Cuts at: 3781 3781
Size: 6761
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AloI ApaI AscI AvrII BaeI
BmgBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI NheI NotI
NruI PacI PasI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI