Restriction map for
pF1KA0579
(Circular) (Six-base) MAPSORT of: pF1KA0579.seq Check: 3402 from: 1 to: 5960
pF1KA0579 5960 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 138 960 138
Size: 822 5138
AccI GT'mk_AC
Cuts at: 2998 2998
Size: 5960
Acc65I G'GTAC_C
Cuts at: 647 772 2976 647
Size: 125 2204 3631
Fragments arranged by size:
3631 2204 125
AclI AA'CG_TT
Cuts at: 5800 5800
Size: 5960
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 433 533 575 715 1115 1945 2571 3785
Size: 319 100 42 140 400 830 626 1214
Cuts at: 3785 4217 4570 5232 114
Size: 432 353 662 842
Fragments arranged by size:
1214 842 830 662 626 432 400 353
319 140 100 42
AfeI AGC'GCT
Cuts at: 1422 1422
Size: 5960
AflIII A'CryG_T
Cuts at: 671 1440 3312 5112 5393 671
Size: 769 1872 1800 281 1238
Fragments arranged by size:
1872 1800 1238 769 281
AlwNI CAG_nnn'CTG
Cuts at: 153 1599 2692 3265 4703 153
Size: 1446 1093 573 1438 1410
Fragments arranged by size:
1446 1438 1410 1093 573
ApaI G_GGCC'C
Cuts at: 1268 2191 1268
Size: 923 5037
ApaLI G'TGCA_C
Cuts at: 1645 1696 2469 2656 4798 1645
Size: 51 773 187 2142 2807
Fragments arranged by size:
2807 2142 773 187 51
ApoI r'AATT_y
Cuts at: 523 1111 1638 2964 5313 5569 523
Size: 588 527 1326 2349 256 914
Fragments arranged by size:
2349 1326 914 588 527 256
AscI GG'CGCG_CC
Cuts at: 218 218
Size: 5960
AseI AT'TA_AT
Cuts at: 20 20
Size: 5960
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5960
AvaI C'yCGr_G
Cuts at: 223 1238 1340 1400 2502 2980 223
Size: 1015 102 60 1102 478 3203
Fragments arranged by size:
3203 1102 1015 478 102 60
BamHI G'GATC_C
Cuts at: 2985 2985
Size: 5960
BanI G'GyrC_C
Cuts at: 647 772 1190 2427 2976 3390 3638 3673 647
Size: 125 418 1237 549 414 248 35 2934
Fragments arranged by size:
2934 1237 549 418 414 248 125 35
BanII G_rGCy'C
Cuts at: 167 843 916 1268 1414 1465 2191 2974 4004
Size: 676 73 352 146 51 726 783 1030
Cuts at: 4004 167
Size: 2123
Fragments arranged by size:
2123 1030 783 726 676 352 146 73
51
BbeI G_GCGC'C
Cuts at: 3394 3642 3394
Size: 248 5712
BbsI GAAGACnn'nnnn_
Cuts at: 1682 1933 1682
Size: 251 5709
BbvCI CC'TCA_GC
Cuts at: 1424 1424
Size: 5960
BciVI GTATCCnnnnn_n'
Cuts at: 829 3858 4914 5960 829
Size: 3029 1056 1046 829
Fragments arranged by size:
3029 1056 1046 829
BclI T'GATC_A
Cuts at: 3480 3480
Size: 5960
BfrBI ATG'CAT
Cuts at: 1903 1903
Size: 5960
BglI GCCn_nnn'nGGC
Cuts at: 191 209 366 2266 191
Size: 18 157 1900 3885
Fragments arranged by size:
3885 1900 157 18
BglII A'GATC_T
Cuts at: 1101 3475 5512 1101
Size: 2374 2037 1549
Fragments arranged by size:
2374 2037 1549
BlpI GC'TnA_GC
Cuts at: 585 915 1600 3072 585
Size: 330 685 1472 3473
Fragments arranged by size:
3473 1472 685 330
Bme1580I G_kGCm'C
Cuts at: 1247 1268 1649 1700 2105 2191 2473 2660 3585
Size: 21 381 51 405 86 282 187 925
Cuts at: 3585 3678 4802 1247
Size: 93 1124 2405
Fragments arranged by size:
2405 1124 925 405 381 282 187 93
86 51 21
BmgBI CAC'GTC
Cuts at: 1260 1441 1791 2252 2474 1260
Size: 181 350 461 222 4746
Fragments arranged by size:
4746 461 350 222 181
BmrI ACTGGGnnnn_n'
Cuts at: 3172 3253 3588 5732 3172
Size: 81 335 2144 3400
Fragments arranged by size:
3400 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1575 1869 4327 1575
Size: 294 2458 3208
Fragments arranged by size:
3208 2458 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1350 1362 2283 2912 1180
Size: 170 12 921 629 4228
Fragments arranged by size:
4228 921 629 170 12
Bpu10I CC'TnA_GC
Cuts at: 1021 1424 1021
Size: 403 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 3136 4015 4729 5027 1132
Size: 2004 879 714 298 2065
Fragments arranged by size:
2065 2004 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1951 5664 1951
Size: 3713 2247
BsaAI yAC'GTr
Cuts at: 624 672 3313 624
Size: 48 2641 3271
Fragments arranged by size:
3271 2641 48
BsaBI GATnn'nnATC
Cuts at: 6 3474 5511 6
Size: 3468 2037 455
Fragments arranged by size:
3468 2037 455
BsaHI Gr'CG_yC
Cuts at: 1981 3391 3639 4341 5837 1981
Size: 1410 248 702 1496 2104
Fragments arranged by size:
2104 1496 1410 702 248
BsaWI w'CCGG_w
Cuts at: 594 929 2551 3149 3670 4348 4759 4906 5180
Size: 335 1622 598 521 678 411 147 274
Cuts at: 5180 594
Size: 1374
Fragments arranged by size:
1622 1374 678 598 521 411 335 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 699 729 1354 1384 2214 2244 2533 2563 699
Size: 30 625 30 830 30 289 30 4096
Fragments arranged by size:
4096 830 625 289 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 1362 1365 1441 1672 364
Size: 998 3 76 231 4652
Fragments arranged by size:
4652 998 231 76 3
BseYI C'CCAG_C
Cuts at: 252 842 1154 1984 2233 2278 2286 2293 2621
Size: 590 312 830 249 45 8 7 328
Cuts at: 2621 3385 4143 4808 5216 252
Size: 764 758 665 408 996
Fragments arranged by size:
996 830 764 758 665 590 408 328
312 249 45 8 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1291 1564 1843 2619 310
Size: 981 273 279 776 3651
Fragments arranged by size:
3651 981 776 279 273
BsiEI CG_ry'CG
Cuts at: 104 866 1970 3502 3548 4778 104
Size: 762 1104 1532 46 1230 1286
Fragments arranged by size:
1532 1286 1230 1104 762 46
BsiHKAI G_wGCw'C
Cuts at: 167 1414 1465 1649 1700 2473 2660 2974 3752
Size: 1247 51 184 51 773 187 314 778
Cuts at: 3752 3942 4802 167
Size: 190 860 1325
Fragments arranged by size:
1325 1247 860 778 773 314 190 187
184 51 51
BsmI GAATG_Cn'
Cuts at: 324 324
Size: 5960
BsmBI CGTCTCn'nnnn_
Cuts at: 1179 1179
Size: 5960
Bsp1286I G_dGCh'C
Cuts at: 167 843 916 1247 1268 1414 1465 1649 1700
Size: 676 73 331 21 146 51 184 51
Cuts at: 1700 2105 2191 2473 2660 2974 3585 3678 3752
Size: 405 86 282 187 314 611 93 74
Cuts at: 3752 3942 4004 4802 167
Size: 190 62 798 1325
Fragments arranged by size:
1325 798 676 611 405 331 314 282
190 187 184 146 93 86 74 73
62 51 51 21
BspEI T'CCGG_A
Cuts at: 594 594
Size: 5960
BspHI T'CATG_A
Cuts at: 4392 4392
Size: 5960
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 960 1815 3010 3224 3526 3907 138
Size: 822 855 1195 214 302 381 2191
Fragments arranged by size:
2191 1195 855 822 381 302 214
BsrBI CCG'CTC
Cuts at: 50 836 2816 4252 4306 5790 5955 50
Size: 786 1980 1436 54 1484 165 55
Fragments arranged by size:
1980 1484 1436 786 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2174 2799 3872 2174
Size: 625 1073 4262
Fragments arranged by size:
4262 1073 625
BsrFI r'CCGG_y
Cuts at: 865 1479 1941 2894 3958 4139 865
Size: 614 462 953 1064 181 2686
Fragments arranged by size:
2686 1064 953 614 462 181
BsrGI T'GTAC_A
Cuts at: 2135 2135
Size: 5960
BssHII G'CGCG_C
Cuts at: 218 236 218
Size: 18 5942
BssSI C'ACGA_G
Cuts at: 4231 4939 4231
Size: 708 5252
BstAPI GCAn_nnn'nTGC
Cuts at: 153 2468 3337 153
Size: 2315 869 2776
Fragments arranged by size:
2776 2315 869
BstBI TT'CG_AA
Cuts at: 4321 4321
Size: 5960
BstEII G'GTnAC_C
Cuts at: 1218 2851 1218
Size: 1633 4327
BstXI CCAn_nnnn'nTGG
Cuts at: 1386 1386
Size: 5960
BstYI r'GATC_y
Cuts at: 1 173 627 1101 1732 2985 3452 3475 3810
Size: 172 454 474 631 1253 467 23 335
Cuts at: 3810 4056 4362 4374 4460 5512 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1253 1052 631 474 467 454 449 335
306 246 172 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 372 643 372
Size: 271 5689
BtgI C'CryG_G
Cuts at: 107 204 344 849 1070 1127 1385 1523 1973
Size: 97 140 505 221 57 258 138 450
Cuts at: 1973 2882 107
Size: 909 3185
Fragments arranged by size:
3185 909 505 450 258 221 140 138
97 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3910 4091 4254 3910
Size: 181 163 5616
Fragments arranged by size:
5616 181 163
BtsI GCAGTG_nn'
Cuts at: 303 888 1233 2222 2352 3203 303
Size: 585 345 989 130 851 3060
Fragments arranged by size:
3060 989 851 585 345 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2238 2273 2238
Size: 35 5925
DraI TTT'AAA
Cuts at: 2959 2959
Size: 5960
DrdI GACnn_nn'nnGTC
Cuts at: 3666 5010 3666
Size: 1344 4616
EaeI y'GGCC_r
Cuts at: 863 1967 2267 2896 3545 3719 4110 4137 5482
Size: 1104 300 629 649 174 391 27 1345
Cuts at: 5482 863
Size: 1341
Fragments arranged by size:
1345 1341 1104 649 629 391 300 174
27
EagI C'GGCC_G
Cuts at: 863 1967 3545 863
Size: 1104 1578 3278
Fragments arranged by size:
3278 1578 1104
EarI CTCTTCn'nnn_
Cuts at: 551 1400 3983 4193 551
Size: 849 2583 210 2318
Fragments arranged by size:
2583 2318 849 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 861 1503 2000 4168 4910 5056 5770 861
Size: 642 497 2168 742 146 714 1051
Fragments arranged by size:
2168 1051 742 714 642 497 146
EcoICRI GAG'CTC
Cuts at: 165 1412 1463 2972 165
Size: 1247 51 1509 3153
Fragments arranged by size:
3153 1509 1247 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 433 533 575 715 1115 1180 1350 1362
Size: 319 100 42 140 400 65 170 12
Cuts at: 1362 1945 2283 2571 2912 3785 4217 4570 5232
Size: 583 338 288 341 873 432 353 662
Cuts at: 5232 114
Size: 842
Fragments arranged by size:
873 842 662 583 432 400 353 341
338 319 288 170 140 100 65 42
12
EcoO109I rG'GnC_Cy
Cuts at: 285 368 1827 2188 2681 3099 285
Size: 83 1459 361 493 418 3146
Fragments arranged by size:
3146 1459 493 418 361 83
EcoRI G'AATT_C
Cuts at: 1638 2964 1638
Size: 1326 4634
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 387 3979 387
Size: 3592 2368
FspI TGC'GCA
Cuts at: 3741 3741
Size: 5960
HaeII r_GCGC'y
Cuts at: 1424 1781 2311 2621 3394 3642 4872 5643 1424
Size: 357 530 310 773 248 1230 771 1741
Fragments arranged by size:
1741 1230 773 771 530 357 310 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 726 758 3802 3832 3834 3864 619
Size: 32 75 32 3044 30 2 30 2715
Fragments arranged by size:
3044 2715 75 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2999 2999
Size: 5960
KasI G'GCGC_C
Cuts at: 3390 3638 3390
Size: 248 5712
KpnI G_GTAC'C
Cuts at: 651 776 2980 651
Size: 125 2204 3631
Fragments arranged by size:
3631 2204 125
MluI A'CGCG_T
Cuts at: 5393 5393
Size: 5960
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 1439 4719 4903 140
Size: 1299 3280 184 1197
Fragments arranged by size:
3280 1299 1197 184
MscI TGG'CCA
Cuts at: 2269 3721 2269
Size: 1452 4508
MslI CAynn'nnrTG
Cuts at: 961 1630 1756 3949 4076 961
Size: 669 126 2193 127 2845
Fragments arranged by size:
2845 2193 669 127 126
MspA1I CmG'CkG
Cuts at: 1158 1215 1335 2282 2489 2495 2606 2678 3071
Size: 57 120 947 207 6 111 72 393
Cuts at: 3071 3385 3745 4529 4774 1158
Size: 314 360 784 245 2344
Fragments arranged by size:
2344 947 784 393 360 314 245 207
120 111 72 57 6
NaeI GCC'GGC
Cuts at: 867 4141 867
Size: 3274 2686
NarI GG'CG_CC
Cuts at: 3391 3639 3391
Size: 248 5712
NcoI C'CATG_G
Cuts at: 107 1070 1127 1385 2882 107
Size: 963 57 258 1497 3185
Fragments arranged by size:
3185 1497 963 258 57
NgoMIV G'CCGG_C
Cuts at: 865 4139 865
Size: 3274 2686
NsiI A_TGCA'T
Cuts at: 1905 1905
Size: 5960
NspI r_CATG'y
Cuts at: 1635 1903 3013 3513 5116 1635
Size: 268 1110 500 1603 2479
Fragments arranged by size:
2479 1603 1110 500 268
PciI A'CATG_T
Cuts at: 5112 5112
Size: 5960
PflMI CCAn_nnn'nTGG
Cuts at: 745 2778 745
Size: 2033 3927
PmeI GTTT'AAAC
Cuts at: 2959 2959
Size: 5960
PmlI CAC'GTG
Cuts at: 624 672 624
Size: 48 5912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 4406
Size: 32 5928
PpuMI rG'GwC_Cy
Cuts at: 1827 2681 1827
Size: 854 5106
PshAI GACnn'nnGTC
Cuts at: 1891 1891
Size: 5960
PspOMI G'GGCC_C
Cuts at: 1264 2187 1264
Size: 923 5037
PspXI vC'TCGA_Gb
Cuts at: 1238 2502 1238
Size: 1264 4696
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2781 2813 2781
Size: 32 5928
PstI C_TGCA'G
Cuts at: 980 2198 2488 3007 3692 980
Size: 1218 290 519 685 3248
Fragments arranged by size:
3248 1218 685 519 290
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5960
PvuII CAG'CTG
Cuts at: 1158 1335 2282 2495 3385 3745 1158
Size: 177 947 213 890 360 3373
Fragments arranged by size:
3373 947 890 360 213 177
RsrII CG'GwC_CG
Cuts at: 2554 4155 2554
Size: 1601 4359
SacI G_AGCT'C
Cuts at: 167 1414 1465 2974 167
Size: 1247 51 1509 3153
Fragments arranged by size:
3153 1509 1247 51
SalI G'TCGA_C
Cuts at: 2997 2997
Size: 5960
SanDI GG'GwC_CC
Cuts at: 2681 2681
Size: 5960
SapI GCTCTTCn'nnn_
Cuts at: 3983 4193 3983
Size: 210 5750
SbfI CC_TGCA'GG
Cuts at: 2198 3007 2198
Size: 809 5151
ScaI AGT'ACT
Cuts at: 1363 5477 1363
Size: 4114 1846
SfcI C'TryA_G
Cuts at: 33 976 2194 2337 2484 3003 3688 4656 4847
Size: 943 1218 143 147 519 685 968 191
Cuts at: 4847 33
Size: 1146
Fragments arranged by size:
1218 1146 968 943 685 519 191 147
143
SfoI GGC'GCC
Cuts at: 3392 3640 3392
Size: 248 5712
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5960
SmaI CCC'GGG
Cuts at: 225 1342 1402 2982 225
Size: 1117 60 1580 3203
Fragments arranged by size:
3203 1580 1117 60
SmlI C'TyrA_G
Cuts at: 1147 1238 2502 3115 4030 4744 5006 1147
Size: 91 1264 613 915 714 262 2101
Fragments arranged by size:
2101 1264 915 714 613 262 91
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5960
SphI G_CATG'C
Cuts at: 1635 1903 3013 3513 1635
Size: 268 1110 500 4082
Fragments arranged by size:
4082 1110 500 268
SrfI GCCC'GGGC
Cuts at: 225 225
Size: 5960
StuI AGG'CCT
Cuts at: 641 798 641
Size: 157 5803
StyI C'CwwG_G
Cuts at: 107 1070 1127 1385 2772 2882 3094 3448 107
Size: 963 57 258 1387 110 212 354 2619
Fragments arranged by size:
2619 1387 963 354 258 212 110 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1638 1878 2742 3543 3676 4135 4344 1638
Size: 240 864 801 133 459 209 3254
Fragments arranged by size:
3254 864 801 459 240 209 133
TatI w'GTAC_w
Cuts at: 1361 2135 5475 1361
Size: 774 3340 1846
Fragments arranged by size:
3340 1846 774
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 699 731 785 817 941 973 1489 1521 2214
Size: 32 54 32 124 32 516 32 693
Cuts at: 2214 2246 2533 2565 5477 5509 699
Size: 32 287 32 2912 32 1150
Fragments arranged by size:
2912 1150 693 516 287 124 54 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 688 2692 3757 688
Size: 2004 1065 2891
Fragments arranged by size:
2891 2004 1065
XbaI T'CTAG_A
Cuts at: 1011 1713 2991 1011
Size: 702 1278 3980
Fragments arranged by size:
3980 1278 702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1000 1230 3455 1000
Size: 230 2225 3505
Fragments arranged by size:
3505 2225 230
XhoI C'TCGA_G
Cuts at: 1238 2502 1238
Size: 1264 4696
XmaI C'CCGG_G
Cuts at: 223 1340 1400 2980 223
Size: 1117 60 1580 3203
Fragments arranged by size:
3203 1580 1117 60
XmnI GAAnn'nnTTC
Cuts at: 1498 1498
Size: 5960
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI AvrII
BaeI BcgI BmtI BsiWI BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PsiI SacII SexAI
SfiI SgrAI SnaBI SspI SwaI ZraI