Restriction map for
pF1KA0577
(Circular) (Six-base) MAPSORT of: pF1KA0577.seq Check: 5292 from: 1 to: 6236
pF1KA0577 6236 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2320 3274 2320
Size: 954 5282
Acc65I G'GTAC_C
Cuts at: 202 315 3252 202
Size: 113 2937 3186
Fragments arranged by size:
3186 2937 113
AclI AA'CG_TT
Cuts at: 6076 6076
Size: 6236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1144 1745 4061 4493 5240 5508 607
Size: 537 601 2316 432 747 268 1335
Fragments arranged by size:
2316 1335 747 601 537 432 268
AfeI AGC'GCT
Cuts at: 133 214 133
Size: 81 6155
AflIII A'CryG_T
Cuts at: 2877 3588 4692 5669 2877
Size: 711 1104 977 3444
Fragments arranged by size:
3444 1104 977 711
AgeI A'CCGG_T
Cuts at: 4624 4624
Size: 6236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 231 2322 2513 231
Size: 2091 191 3954
Fragments arranged by size:
3954 2091 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2170 2202 2170
Size: 32 6204
AlwNI CAG_nnn'CTG
Cuts at: 195 630 1873 2365 2638 2900 3108 3541 5108
Size: 435 1243 492 273 262 208 433 1567
Cuts at: 5108 195
Size: 1323
Fragments arranged by size:
1567 1323 1243 492 435 433 273 262
208
ApaI G_GGCC'C
Cuts at: 375 1137 375
Size: 762 5474
ApaLI G'TGCA_C
Cuts at: 5006 5006
Size: 6236
ApoI r'AATT_y
Cuts at: 3240 5589 5845 3240
Size: 2349 256 3631
Fragments arranged by size:
3631 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6236
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6236
AvaI C'yCGr_G
Cuts at: 284 347 368 947 1019 1034 1136 1496 1505
Size: 63 21 579 72 15 102 360 9
Cuts at: 1505 2840 3256 284
Size: 1335 416 3264
Fragments arranged by size:
3264 1335 579 416 360 102 72 63
21 15 9
BamHI G'GATC_C
Cuts at: 2178 2420 3261 2178
Size: 242 841 5153
Fragments arranged by size:
5153 841 242
BanI G'GyrC_C
Cuts at: 202 315 3252 3666 3914 3949 202
Size: 113 2937 414 248 35 2489
Fragments arranged by size:
2937 2489 414 248 113 35
BanII G_rGCy'C
Cuts at: 375 917 1071 1137 1160 1331 1745 2017 2033
Size: 542 154 66 23 171 414 272 16
Cuts at: 2033 2511 2531 2841 2870 3250 4280 375
Size: 478 20 310 29 380 1030 2331
Fragments arranged by size:
2331 1030 542 478 414 380 310 272
171 154 66 29 23 20 16
BbeI G_GCGC'C
Cuts at: 3670 3918 3670
Size: 248 5988
BbsI GAAGACnn'nnnn_
Cuts at: 416 764 1396 3003 416
Size: 348 632 1607 3649
Fragments arranged by size:
3649 1607 632 348
BbvCI CC'TCA_GC
Cuts at: 2259 2259
Size: 6236
BciVI GTATCCnnnnn_n'
Cuts at: 4134 4895 6236 4134
Size: 761 1341 4134
Fragments arranged by size:
4134 1341 761
BclI T'GATC_A
Cuts at: 1712 3756 1712
Size: 2044 4192
BglI GCCn_nnn'nGGC
Cuts at: 1501 2012 3054 1501
Size: 511 1042 4683
Fragments arranged by size:
4683 1042 511
BglII A'GATC_T
Cuts at: 3751 5788 3751
Size: 2037 4199
BlpI GC'TnA_GC
Cuts at: 169 653 815 892 3348 169
Size: 484 162 77 2456 3057
Fragments arranged by size:
3057 2456 484 162 77
Bme1580I G_kGCm'C
Cuts at: 375 1137 2109 3861 3954 5010 375
Size: 762 972 1752 93 1056 1601
Fragments arranged by size:
1752 1601 1056 972 762 93
BmgBI CAC'GTC
Cuts at: 183 885 2537 183
Size: 702 1652 3882
Fragments arranged by size:
3882 1652 702
BmrI ACTGGGnnnn_n'
Cuts at: 183 2787 3448 3529 3864 6008 183
Size: 2604 661 81 335 2144 411
Fragments arranged by size:
2604 2144 661 411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 171 1345 1398 1641 4603 171
Size: 1174 53 243 2962 1804
Fragments arranged by size:
2962 1804 1174 243 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 400 445 652 865 1003 1198 1204 1850
Size: 252 45 207 213 138 195 6 646
Cuts at: 1850 1975 3130 3154 148
Size: 125 1155 24 3230
Fragments arranged by size:
3230 1155 646 252 213 207 195 138
125 45 24 6
Bpu10I CC'TnA_GC
Cuts at: 779 1383 1739 2259 2415 779
Size: 604 356 520 156 4600
Fragments arranged by size:
4600 604 520 356 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 856 1729 2368 2392 3412 4291 4783 5081
Size: 129 873 639 24 1020 879 492 298
Cuts at: 5081 727
Size: 1882
Fragments arranged by size:
1882 1020 879 873 639 492 298 129
24
BsaI GGTCTCn'nnnn_
Cuts at: 1183 2896 5940 1183
Size: 1713 3044 1479
Fragments arranged by size:
3044 1713 1479
BsaAI yAC'GTr
Cuts at: 3589 3589
Size: 6236
BsaBI GATnn'nnATC
Cuts at: 6 3750 5787 6
Size: 3744 2037 455
Fragments arranged by size:
3744 2037 455
BsaHI Gr'CG_yC
Cuts at: 115 1805 3667 3915 4617 6113 115
Size: 1690 1862 248 702 1496 238
Fragments arranged by size:
1862 1690 1496 702 248 238
BsaWI w'CCGG_w
Cuts at: 761 3425 3946 4624 4898 5045 5456 761
Size: 2664 521 678 274 147 411 1541
Fragments arranged by size:
2664 1541 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1147 1177 1848 1878 2479 2509 1147
Size: 30 671 30 601 30 4874
Fragments arranged by size:
4874 671 601 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 445 529 649 880 1069 1093 1111 1195
Size: 201 84 120 231 189 24 18 84
Cuts at: 1195 1198 1201 1246 1339 1435 1978 2635 3018
Size: 3 3 45 93 96 543 657 383
Cuts at: 3018 244
Size: 3462
Fragments arranged by size:
3462 657 543 383 231 201 189 120
96 93 84 84 45 24 18 3
3
BseYI C'CCAG_C
Cuts at: 134 964 1091 1265 1922 3661 4419 4996 5492
Size: 830 127 174 657 1739 758 577 496
Cuts at: 5492 134
Size: 878
Fragments arranged by size:
1739 878 830 758 657 577 496 174
127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 201 259 1440 1548 136
Size: 65 58 1181 108 4824
Fragments arranged by size:
4824 1181 108 65 58
BsiEI CG_ry'CG
Cuts at: 104 3778 3824 5032 104
Size: 3674 46 1208 1308
Fragments arranged by size:
3674 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 917 1331 1745 2033 2531 3250 4028 4218 5010
Size: 414 414 288 498 719 778 190 792
Cuts at: 5010 917
Size: 2143
Fragments arranged by size:
2143 792 778 719 498 414 414 288
190
Bsp1286I G_dGCh'C
Cuts at: 375 917 1071 1137 1160 1331 1745 2017 2033
Size: 542 154 66 23 171 414 272 16
Cuts at: 2033 2109 2511 2531 2841 2870 3250 3861 3954
Size: 76 402 20 310 29 380 611 93
Cuts at: 3954 4028 4218 4280 5010 375
Size: 74 190 62 730 1601
Fragments arranged by size:
1601 730 611 542 414 402 380 310
272 190 171 154 93 76 74 66
62 29 23 20 16
BspEI T'CCGG_A
Cuts at: 5456 5456
Size: 6236
BspHI T'CATG_A
Cuts at: 3095 5412 3095
Size: 2317 3919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1823 2275 3286 3500 3802 4183 1823
Size: 452 1011 214 302 381 3876
Fragments arranged by size:
3876 1011 452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 177 657 906 4528 4582 6066 6231 50
Size: 127 480 249 3622 54 1484 165 55
Fragments arranged by size:
3622 1484 480 249 165 127 55 54
BsrDI GCAATG_nn'
Cuts at: 2132 2397 4148 2132
Size: 265 1751 4220
Fragments arranged by size:
4220 1751 265
BsrFI r'CCGG_y
Cuts at: 117 279 896 2562 4234 4415 4624 117
Size: 162 617 1666 1672 181 209 1729
Fragments arranged by size:
1729 1672 1666 617 209 181 162
BsrGI T'GTAC_A
Cuts at: 2814 2814
Size: 6236
BssSI C'ACGA_G
Cuts at: 901 2341 3215 4507 4865 901
Size: 1440 874 1292 358 2272
Fragments arranged by size:
2272 1440 1292 874 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3613 3613
Size: 6236
BstBI TT'CG_AA
Cuts at: 1818 4597 1818
Size: 2779 3457
BstEII G'GTnAC_C
Cuts at: 2734 2734
Size: 6236
BstXI CCAn_nnnn'nTGG
Cuts at: 1488 1930 1488
Size: 442 5794
BstYI r'GATC_y
Cuts at: 1 267 939 1044 1400 1910 2178 2369 2420
Size: 266 672 105 356 510 268 191 51
Cuts at: 2420 2624 3174 3261 3728 3751 4086 4332 5344
Size: 204 550 87 467 23 335 246 1012
Cuts at: 5344 5430 5442 5788 1
Size: 86 12 346 449
Fragments arranged by size:
1012 672 550 510 467 449 356 346
335 268 266 246 204 191 105 87
86 51 23 12
BstZ17I GTA'TAC
Cuts at: 2321 2321
Size: 6236
Bsu36I CC'TnA_GG
Cuts at: 490 490
Size: 6236
BtgI C'CryG_G
Cuts at: 107 770 107
Size: 663 5573
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4186 4367 4530 4186
Size: 181 163 5892
Fragments arranged by size:
5892 181 163
BtsI GCAGTG_nn'
Cuts at: 335 432 1775 2470 2936 3479 335
Size: 97 1343 695 466 543 3092
Fragments arranged by size:
3092 1343 695 543 466 97
ClaI AT'CG_AT
Cuts at: 2827 2827
Size: 6236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1779 1814 1779
Size: 35 6201
DraI TTT'AAA
Cuts at: 3235 3235
Size: 6236
DraIII CAC_nnn'GTG
Cuts at: 159 2768 159
Size: 2609 3627
DrdI GACnn_nn'nnGTC
Cuts at: 2689 3942 4800 2689
Size: 1253 858 4125
Fragments arranged by size:
4125 1253 858
EaeI y'GGCC_r
Cuts at: 749 1490 3821 3995 4386 4413 5758 749
Size: 741 2331 174 391 27 1345 1227
Fragments arranged by size:
2331 1345 1227 741 391 174 27
EagI C'GGCC_G
Cuts at: 3821 3821
Size: 6236
EarI CTCTTCn'nnn_
Cuts at: 413 506 515 719 2194 2793 3215 4259 4469
Size: 93 9 204 1475 599 422 1044 210
Cuts at: 4469 413
Size: 2180
Fragments arranged by size:
2180 1475 1044 599 422 210 204 93
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 865 1282 2302 4444 4754 4900 6046 865
Size: 417 1020 2142 310 146 1146 1055
Fragments arranged by size:
2142 1146 1055 1020 417 310 146
EcoICRI GAG'CTC
Cuts at: 915 1329 1743 2031 2529 3248 915
Size: 414 414 288 498 719 3903
Fragments arranged by size:
3903 719 498 414 414 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 400 445 607 652 865 1003 1144 1198
Size: 252 45 162 45 213 138 141 54
Cuts at: 1198 1204 1745 1850 1975 3130 3154 4061 4493
Size: 6 541 105 125 1155 24 907 432
Cuts at: 4493 5240 5508 148
Size: 747 268 876
Fragments arranged by size:
1155 907 876 747 541 432 268 252
213 162 141 138 125 105 54 45
45 24 6
EcoO109I rG'GnC_Cy
Cuts at: 372 627 840 1133 1682 1749 3375 372
Size: 255 213 293 549 67 1626 3233
Fragments arranged by size:
3233 1626 549 293 255 213 67
EcoRI G'AATT_C
Cuts at: 3240 3240
Size: 6236
EcoRV GAT'ATC
Cuts at: 1938 1938
Size: 6236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4255 4255
Size: 6236
FseI GG_CCGG'CC
Cuts at: 283 283
Size: 6236
FspI TGC'GCA
Cuts at: 4017 4017
Size: 6236
HaeII r_GCGC'y
Cuts at: 135 216 246 741 1092 3670 3918 4940 5919
Size: 81 30 495 351 2578 248 1022 979
Cuts at: 5919 135
Size: 452
Fragments arranged by size:
2578 1022 979 495 452 351 248 81
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1045 1077 1132 1164 1222 1254 1552 1584 1727
Size: 32 55 32 58 32 298 32 143
Cuts at: 1727 1759 4078 4108 4110 4140 1045
Size: 32 2319 30 2 30 3141
Fragments arranged by size:
3141 2319 298 143 58 55 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2655 2715 2969 3275 2655
Size: 60 254 306 5616
Fragments arranged by size:
5616 306 254 60
HindIII A'AGCT_T
Cuts at: 832 1522 1886 832
Size: 690 364 5182
Fragments arranged by size:
5182 690 364
KasI G'GCGC_C
Cuts at: 3666 3914 3666
Size: 248 5988
KpnI G_GTAC'C
Cuts at: 206 319 3256 206
Size: 113 2937 3186
Fragments arranged by size:
3186 2937 113
MluI A'CGCG_T
Cuts at: 5669 5669
Size: 6236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 1758 4907 5091 566
Size: 1192 3149 184 1711
Fragments arranged by size:
3149 1711 1192 184
MscI TGG'CCA
Cuts at: 3997 3997
Size: 6236
MslI CAynn'nnrTG
Cuts at: 1486 2170 2221 4225 4352 1486
Size: 684 51 2004 127 3370
Fragments arranged by size:
3370 2004 684 127 51
MspA1I CmG'CkG
Cuts at: 669 964 1086 2493 2612 2859 3347 3661 4021
Size: 295 122 1407 119 247 488 314 360
Cuts at: 4021 5034 5279 669
Size: 1013 245 1626
Fragments arranged by size:
1626 1407 1013 488 360 314 295 247
245 122 119
NaeI GCC'GGC
Cuts at: 119 281 898 4417 119
Size: 162 617 3519 1938
Fragments arranged by size:
3519 1938 617 162
NarI GG'CG_CC
Cuts at: 3667 3915 3667
Size: 248 5988
NcoI C'CATG_G
Cuts at: 107 770 107
Size: 663 5573
NdeI CA'TA_TG
Cuts at: 2463 2463
Size: 6236
NgoMIV G'CCGG_C
Cuts at: 117 279 896 4415 117
Size: 162 617 3519 1938
Fragments arranged by size:
3519 1938 617 162
NspI r_CATG'y
Cuts at: 1008 2073 3289 3789 4696 1008
Size: 1065 1216 500 907 2548
Fragments arranged by size:
2548 1216 1065 907 500
PasI CC'CwG_GG
Cuts at: 1072 1930 1072
Size: 858 5378
PciI A'CATG_T
Cuts at: 4692 4692
Size: 6236
PfoI T'CCnGG_A
Cuts at: 695 1613 1991 2024 2447 695
Size: 918 378 33 423 4484
Fragments arranged by size:
4484 918 423 378 33
PmeI GTTT'AAAC
Cuts at: 3235 3235
Size: 6236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5375 5407 5375
Size: 32 6204
PpuMI rG'GwC_Cy
Cuts at: 627 840 1682 1749 627
Size: 213 842 67 5114
Fragments arranged by size:
5114 842 213 67
PsiI TTA'TAA
Cuts at: 3155 3155
Size: 6236
PspOMI G'GGCC_C
Cuts at: 371 1133 371
Size: 762 5474
PstI C_TGCA'G
Cuts at: 2251 2301 3283 3968 2251
Size: 50 982 685 4519
Fragments arranged by size:
4519 982 685 50
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6236
PvuII CAG'CTG
Cuts at: 964 2493 2612 2859 3661 4021 964
Size: 1529 119 247 802 360 3179
Fragments arranged by size:
3179 1529 802 360 247 119
RsrII CG'GwC_CG
Cuts at: 4431 4431
Size: 6236
SacI G_AGCT'C
Cuts at: 917 1331 1745 2033 2531 3250 917
Size: 414 414 288 498 719 3903
Fragments arranged by size:
3903 719 498 414 414 288
SalI G'TCGA_C
Cuts at: 3273 3273
Size: 6236
SapI GCTCTTCn'nnn_
Cuts at: 2194 3215 4259 4469 2194
Size: 1021 1044 210 3961
Fragments arranged by size:
3961 1044 1021 210
SbfI CC_TGCA'GG
Cuts at: 3283 3283
Size: 6236
ScaI AGT'ACT
Cuts at: 5753 5753
Size: 6236
SexAI A'CCwGG_T
Cuts at: 2741 2741
Size: 6236
SfcI C'TryA_G
Cuts at: 33 1542 2247 2297 3279 3964 4957 5148 33
Size: 1509 705 50 982 685 993 191 1121
Fragments arranged by size:
1509 1121 993 982 705 685 191 50
SfoI GGC'GCC
Cuts at: 3668 3916 3668
Size: 248 5988
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6236
SgrAI Cr'CCGG_yG
Cuts at: 117 117
Size: 6236
SmaI CCC'GGG
Cuts at: 286 370 949 1507 2842 3258 286
Size: 84 579 558 1335 416 3264
Fragments arranged by size:
3264 1335 579 558 416 84
SmlI C'TyrA_G
Cuts at: 742 835 1744 2347 2407 3391 4306 4798 5060
Size: 93 909 603 60 984 915 492 262
Cuts at: 5060 742
Size: 1918
Fragments arranged by size:
1918 984 915 909 603 492 262 93
60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6236
SphI G_CATG'C
Cuts at: 3289 3789 3289
Size: 500 5736
SrfI GCCC'GGGC
Cuts at: 370 370
Size: 6236
StuI AGG'CCT
Cuts at: 2257 2257
Size: 6236
StyI C'CwwG_G
Cuts at: 107 263 770 1010 1859 2520 3161 3370 3724
Size: 156 507 240 849 661 641 209 354
Cuts at: 3724 107
Size: 2619
Fragments arranged by size:
2619 849 661 641 507 354 240 209
156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1411 1474 1504 3819 3952 4411 4620 1263
Size: 148 63 30 2315 133 459 209 2879
Fragments arranged by size:
2879 2315 459 209 148 133 63 30
TatI w'GTAC_w
Cuts at: 2606 2814 5751 2606
Size: 208 2937 3091
Fragments arranged by size:
3091 2937 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 486 518 580 597 612 629 1848 1880 5753
Size: 32 62 17 15 17 1219 32 3873
Cuts at: 5753 5785 486
Size: 32 937
Fragments arranged by size:
3873 1219 937 62 32 32 32 17
17 15
Tth111I GACn'n_nGTC
Cuts at: 2686 4033 2686
Size: 1347 4889
XbaI T'CTAG_A
Cuts at: 3267 3267
Size: 6236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1167 3731 1167
Size: 2564 3672
XmaI C'CCGG_G
Cuts at: 284 368 947 1505 2840 3256 284
Size: 84 579 558 1335 416 3264
Fragments arranged by size:
3264 1335 579 558 416 84
XmnI GAAnn'nnTTC
Cuts at: 620 2308 620
Size: 1688 4548
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BssHII EcoNI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PflMI PmlI PshAI PspXI PsrI SacII SanDI SfiI
SnaBI SspI SwaI XhoI ZraI