Restriction map for
pF1KA0575
(Circular) (Six-base) MAPSORT of: pF1KA0575.seq Check: 7757 from: 1 to: 5954
pF1KA0575 5954 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2330 2621 2992 2330
Size: 291 371 5292
Fragments arranged by size:
5292 371 291
Acc65I G'GTAC_C
Cuts at: 2970 2970
Size: 5954
AclI AA'CG_TT
Cuts at: 5794 5794
Size: 5954
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 1641 2370 2611 3779 4211 4958 5226 171
Size: 1470 729 241 1168 432 747 268 899
Fragments arranged by size:
1470 1168 899 747 729 432 268 241
AfeI AGC'GCT
Cuts at: 521 521
Size: 5954
AflIII A'CryG_T
Cuts at: 2117 2510 3306 4410 5387 2117
Size: 393 796 1104 977 2684
Fragments arranged by size:
2684 1104 977 796 393
AgeI A'CCGG_T
Cuts at: 2309 4342 2309
Size: 2033 3921
AleI CACnn'nnGTG
Cuts at: 1213 1505 1213
Size: 292 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 946 1141 1592 946
Size: 195 451 5308
Fragments arranged by size:
5308 451 195
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2695 2727 2695
Size: 32 5922
AlwNI CAG_nnn'CTG
Cuts at: 294 2538 3259 4826 294
Size: 2244 721 1567 1422
Fragments arranged by size:
2244 1567 1422 721
ApaLI G'TGCA_C
Cuts at: 2251 2347 4724 2251
Size: 96 2377 3481
Fragments arranged by size:
3481 2377 96
ApoI r'AATT_y
Cuts at: 2958 5307 5563 2958
Size: 2349 256 3349
Fragments arranged by size:
3349 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5954
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5954
AvaI C'yCGr_G
Cuts at: 638 682 777 1966 2210 2854 2974 638
Size: 44 95 1189 244 644 120 3618
Fragments arranged by size:
3618 1189 644 244 120 95 44
BamHI G'GATC_C
Cuts at: 2979 2979
Size: 5954
BanI G'GyrC_C
Cuts at: 801 1050 1159 1801 2970 3384 3632 3667 801
Size: 249 109 642 1169 414 248 35 3088
Fragments arranged by size:
3088 1169 642 414 249 248 109 35
BanII G_rGCy'C
Cuts at: 618 784 1775 1967 2234 2671 2824 2855 2968
Size: 166 991 192 267 437 153 31 113
Cuts at: 2968 3998 618
Size: 1030 2574
Fragments arranged by size:
2574 1030 991 437 267 192 166 153
113 31
BbeI G_GCGC'C
Cuts at: 805 3388 3636 805
Size: 2583 248 3123
Fragments arranged by size:
3123 2583 248
BbsI GAAGACnn'nnnn_
Cuts at: 870 1159 1412 1647 2918 870
Size: 289 253 235 1271 3906
Fragments arranged by size:
3906 1271 289 253 235
BciVI GTATCCnnnnn_n'
Cuts at: 3852 4613 5954 3852
Size: 761 1341 3852
Fragments arranged by size:
3852 1341 761
BclI T'GATC_A
Cuts at: 3474 3474
Size: 5954
BglII A'GATC_T
Cuts at: 3469 5506 3469
Size: 2037 3917
BlpI GC'TnA_GC
Cuts at: 382 542 997 1129 1285 3066 382
Size: 160 455 132 156 1781 3270
Fragments arranged by size:
3270 1781 455 160 156 132
Bme1580I G_kGCm'C
Cuts at: 1055 2255 2351 2496 3579 3672 4728 1055
Size: 1200 96 145 1083 93 1056 2281
Fragments arranged by size:
2281 1200 1083 1056 145 96 93
BmrI ACTGGGnnnn_n'
Cuts at: 831 1886 2475 2602 3166 3247 3582 5726 831
Size: 1055 589 127 564 81 335 2144 1059
Fragments arranged by size:
2144 1059 1055 589 564 335 127 81
BmtI G_CTAG'C
Cuts at: 2759 2759
Size: 5954
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2253 4321 2253
Size: 2068 3886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 1264 1327 1688 1699 1931 1981 2563 2655
Size: 1083 63 361 11 232 50 582 92
Cuts at: 2655 2869 181
Size: 214 3266
Fragments arranged by size:
3266 1083 582 361 232 214 92 63
50 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3130 4009 4501 4799 3130
Size: 879 492 298 4285
Fragments arranged by size:
4285 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 132 5658 132
Size: 5526 428
BsaAI yAC'GTr
Cuts at: 818 1818 2250 3307 818
Size: 1000 432 1057 3465
Fragments arranged by size:
3465 1057 1000 432
BsaBI GATnn'nnATC
Cuts at: 6 1243 3468 5505 6
Size: 1237 2225 2037 455
Fragments arranged by size:
2225 2037 1237 455
BsaHI Gr'CG_yC
Cuts at: 691 802 1449 3385 3633 4335 5831 691
Size: 111 647 1936 248 702 1496 814
Fragments arranged by size:
1936 1496 814 702 647 248 111
BsaWI w'CCGG_w
Cuts at: 511 2309 2816 3143 3664 4342 4616 4763 5174
Size: 1798 507 327 521 678 274 147 411
Cuts at: 5174 511
Size: 1291
Fragments arranged by size:
1798 1291 678 521 507 411 327 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1728 1758 1728
Size: 30 5924
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 249 421 489 619 741 762 765 780 1246
Size: 172 68 130 122 21 3 15 466
Cuts at: 1246 1249 1618 1999 249
Size: 3 369 381 4204
Fragments arranged by size:
4204 466 381 369 172 130 122 68
21 15 3 3
BseYI C'CCAG_C
Cuts at: 294 411 584 695 761 1060 1989 2077 2181
Size: 117 173 111 66 299 929 88 104
Cuts at: 2181 2531 3379 4137 4714 5210 294
Size: 350 848 758 577 496 1038
Fragments arranged by size:
1038 929 848 758 577 496 350 299
173 117 111 104 88 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 1600 2314 1552
Size: 48 714 5192
Fragments arranged by size:
5192 714 48
BsiEI CG_ry'CG
Cuts at: 104 1758 2471 2840 2920 3496 3542 4750 104
Size: 1654 713 369 80 576 46 1208 1308
Fragments arranged by size:
1654 1308 1208 713 576 369 80 46
BsiHKAI G_wGCw'C
Cuts at: 760 844 1646 1889 1967 2255 2351 2671 2824
Size: 84 802 243 78 288 96 320 153
Cuts at: 2824 2855 2968 3746 3936 4728 760
Size: 31 113 778 190 792 1986
Fragments arranged by size:
1986 802 792 778 320 288 243 190
153 113 96 84 78 31
Bsp1286I G_dGCh'C
Cuts at: 618 760 784 844 1055 1646 1775 1889 1967
Size: 142 24 60 211 591 129 114 78
Cuts at: 1967 2234 2255 2351 2496 2671 2824 2855 2968
Size: 267 21 96 145 175 153 31 113
Cuts at: 2968 3579 3672 3746 3936 3998 4728 618
Size: 611 93 74 190 62 730 1844
Fragments arranged by size:
1844 730 611 591 267 211 190 175
153 145 142 129 114 113 96 93
78 74 62 60 31 24 21
BspEI T'CCGG_A
Cuts at: 5174 5174
Size: 5954
BspHI T'CATG_A
Cuts at: 5130 5130
Size: 5954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 251 1175 2348 3004 3218 3520 3901 251
Size: 924 1173 656 214 302 381 2304
Fragments arranged by size:
2304 1173 924 656 381 302 214
BsrBI CCG'CTC
Cuts at: 50 470 1971 2922 4246 4300 5784 5949 50
Size: 420 1501 951 1324 54 1484 165 55
Fragments arranged by size:
1501 1484 1324 951 420 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1799 3866 1799
Size: 2067 3887
BsrFI r'CCGG_y
Cuts at: 718 811 1037 1753 1976 2309 2356 2372 2475
Size: 93 226 716 223 333 47 16 103
Cuts at: 2475 2915 3952 4133 4342 718
Size: 440 1037 181 209 2330
Fragments arranged by size:
2330 1037 716 440 333 226 223 209
181 103 93 47 16
BssSI C'ACGA_G
Cuts at: 892 1699 4225 4583 892
Size: 807 2526 358 2263
Fragments arranged by size:
2526 2263 807 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2136 3331 2136
Size: 1195 4759
BstBI TT'CG_AA
Cuts at: 4315 4315
Size: 5954
BstEII G'GTnAC_C
Cuts at: 744 744
Size: 5954
BstXI CCAn_nnnn'nTGG
Cuts at: 592 1068 592
Size: 476 5478
BstYI r'GATC_y
Cuts at: 1 1607 2641 2979 3446 3469 3804 4050 5062
Size: 1606 1034 338 467 23 335 246 1012
Cuts at: 5062 5148 5160 5506 1
Size: 86 12 346 449
Fragments arranged by size:
1606 1034 1012 467 449 346 338 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 712 712
Size: 5954
BtgI C'CryG_G
Cuts at: 591 797 2465 591
Size: 206 1668 4080
Fragments arranged by size:
4080 1668 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2897 3904 4085 4248 2897
Size: 1007 181 163 4603
Fragments arranged by size:
4603 1007 181 163
BtsI GCAGTG_nn'
Cuts at: 930 1048 1519 2824 3197 930
Size: 118 471 1305 373 3687
Fragments arranged by size:
3687 1305 471 373 118
DraI TTT'AAA
Cuts at: 2687 2953 2687
Size: 266 5688
DraIII CAC_nnn'GTG
Cuts at: 2610 2610
Size: 5954
DrdI GACnn_nn'nnGTC
Cuts at: 3660 4518 3660
Size: 858 5096
EaeI y'GGCC_r
Cuts at: 662 720 1297 1755 2418 2917 3539 3713 4104
Size: 58 577 458 663 499 622 174 391
Cuts at: 4104 4131 5476 662
Size: 27 1345 1140
Fragments arranged by size:
1345 1140 663 622 577 499 458 391
174 58 27
EagI C'GGCC_G
Cuts at: 1755 2917 3539 1755
Size: 1162 622 4170
Fragments arranged by size:
4170 1162 622
EarI CTCTTCn'nnn_
Cuts at: 433 2578 3977 4187 433
Size: 2145 1399 210 2200
Fragments arranged by size:
2200 2145 1399 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 976 2833 4162 4472 4618 5764 976
Size: 1857 1329 310 146 1146 1166
Fragments arranged by size:
1857 1329 1166 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1965 2669 2822 2853 2966 1965
Size: 704 153 31 113 4953
Fragments arranged by size:
4953 704 153 113 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 181 1264 1327 1641 1688 1699 1931 1981
Size: 10 1083 63 314 47 11 232 50
Cuts at: 1981 2370 2563 2611 2655 2869 3779 4211 4958
Size: 389 193 48 44 214 910 432 747
Cuts at: 4958 5226 171
Size: 268 899
Fragments arranged by size:
1083 910 899 747 432 389 314 268
232 214 193 63 50 48 47 44
11 10
EcoNI CCTnn'n_nnAGG
Cuts at: 219 934 1936 219
Size: 715 1002 4237
Fragments arranged by size:
4237 1002 715
EcoO109I rG'GnC_Cy
Cuts at: 464 635 822 1434 2426 2675 3093 464
Size: 171 187 612 992 249 418 3325
Fragments arranged by size:
3325 992 612 418 249 187 171
EcoRI G'AATT_C
Cuts at: 2958 2958
Size: 5954
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1344 3973 1344
Size: 2629 3325
FspI TGC'GCA
Cuts at: 3735 3735
Size: 5954
HaeII r_GCGC'y
Cuts at: 523 805 2357 2527 3388 3636 4658 5637 523
Size: 282 1552 170 861 248 1022 979 840
Fragments arranged by size:
1552 1022 979 861 840 282 248 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 1592 1624 1682 1714 1728 1760 2056
Size: 32 1091 32 58 32 14 32 296
Cuts at: 2056 2088 2806 2838 2930 2962 3796 3826 3828
Size: 32 718 32 92 32 834 30 2
Cuts at: 3828 3858 469
Size: 30 2565
Fragments arranged by size:
2565 1091 834 718 296 92 58 32
32 32 32 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 2070 2622 2993 2070
Size: 552 371 5031
Fragments arranged by size:
5031 552 371
KasI G'GCGC_C
Cuts at: 801 3384 3632 801
Size: 2583 248 3123
Fragments arranged by size:
3123 2583 248
KpnI G_GTAC'C
Cuts at: 2974 2974
Size: 5954
MluI A'CGCG_T
Cuts at: 5387 5387
Size: 5954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1759 1771 2087 4625 4809 1759
Size: 12 316 2538 184 2904
Fragments arranged by size:
2904 2538 316 184 12
MscI TGG'CCA
Cuts at: 1299 3715 1299
Size: 2416 3538
MslI CAynn'nnrTG
Cuts at: 907 1213 1505 1766 1882 3943 4070 907
Size: 306 292 261 116 2061 127 2791
Fragments arranged by size:
2791 2061 306 292 261 127 116
MspA1I CmG'CkG
Cuts at: 765 808 1133 1551 1614 1677 2243 2467 2583
Size: 43 325 418 63 63 566 224 116
Cuts at: 2583 2696 3065 3379 3739 4752 4997 765
Size: 113 369 314 360 1013 245 1722
Fragments arranged by size:
1722 1013 566 418 369 360 325 314
245 224 116 113 63 63 43
NaeI GCC'GGC
Cuts at: 813 2358 2477 4135 813
Size: 1545 119 1658 2632
Fragments arranged by size:
2632 1658 1545 119
NarI GG'CG_CC
Cuts at: 802 3385 3633 802
Size: 2583 248 3123
Fragments arranged by size:
3123 2583 248
NgoMIV G'CCGG_C
Cuts at: 811 2356 2475 4133 811
Size: 1545 119 1658 2632
Fragments arranged by size:
2632 1658 1545 119
NheI G'CTAG_C
Cuts at: 2755 2755
Size: 5954
NspI r_CATG'y
Cuts at: 574 1689 3007 3507 4414 574
Size: 1115 1318 500 907 2114
Fragments arranged by size:
2114 1318 1115 907 500
PasI CC'CwG_GG
Cuts at: 561 561
Size: 5954
PciI A'CATG_T
Cuts at: 4410 4410
Size: 5954
PflMI CCAn_nnn'nTGG
Cuts at: 1527 1794 1959 1527
Size: 267 165 5522
Fragments arranged by size:
5522 267 165
PfoI T'CCnGG_A
Cuts at: 1189 1420 1189
Size: 231 5723
PmeI GTTT'AAAC
Cuts at: 2953 2953
Size: 5954
PmlI CAC'GTG
Cuts at: 818 1818 2250 818
Size: 1000 432 4522
Fragments arranged by size:
4522 1000 432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1757 1789 2854 2886 5093 5125 1757
Size: 32 1065 32 2207 32 2586
Fragments arranged by size:
2586 2207 1065 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 464 822 1434 2426 464
Size: 358 612 992 3992
Fragments arranged by size:
3992 992 612 358
PshAI GACnn'nnGTC
Cuts at: 2839 2839
Size: 5954
PsiI TTA'TAA
Cuts at: 1853 1853
Size: 5954
PspXI vC'TCGA_Gb
Cuts at: 1966 2854 1966
Size: 888 5066
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2281 2313 2281
Size: 32 5922
PstI C_TGCA'G
Cuts at: 830 1172 2558 3001 3686 830
Size: 342 1386 443 685 3098
Fragments arranged by size:
3098 1386 685 443 342
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5954
PvuII CAG'CTG
Cuts at: 765 1551 1614 1677 2696 3379 3739 765
Size: 786 63 63 1019 683 360 2980
Fragments arranged by size:
2980 1019 786 683 360 63 63
RsrII CG'GwC_CG
Cuts at: 4149 4149
Size: 5954
SacI G_AGCT'C
Cuts at: 1967 2671 2824 2855 2968 1967
Size: 704 153 31 113 4953
Fragments arranged by size:
4953 704 153 113 31
SacII CC_GC'GG
Cuts at: 2468 2468
Size: 5954
SalI G'TCGA_C
Cuts at: 2620 2991 2620
Size: 371 5583
SapI GCTCTTCn'nnn_
Cuts at: 3977 4187 3977
Size: 210 5744
SbfI CC_TGCA'GG
Cuts at: 830 3001 830
Size: 2171 3783
ScaI AGT'ACT
Cuts at: 5471 5471
Size: 5954
SfcI C'TryA_G
Cuts at: 33 705 826 1168 2554 2997 3682 4675 4866
Size: 672 121 342 1386 443 685 993 191
Cuts at: 4866 33
Size: 1121
Fragments arranged by size:
1386 1121 993 685 672 443 342 191
121
SfoI GGC'GCC
Cuts at: 803 3386 3634 803
Size: 2583 248 3123
Fragments arranged by size:
3123 2583 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5954
SmaI CCC'GGG
Cuts at: 640 684 2212 2976 640
Size: 44 1528 764 3618
Fragments arranged by size:
3618 1528 764 44
SmlI C'TyrA_G
Cuts at: 1966 2854 3109 4024 4516 4778 1966
Size: 888 255 915 492 262 3142
Fragments arranged by size:
3142 915 888 492 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5954
SphI G_CATG'C
Cuts at: 574 1689 3007 3507 574
Size: 1115 1318 500 3021
Fragments arranged by size:
3021 1318 1115 500
SspI AAT'ATT
Cuts at: 1861 1861
Size: 5954
StuI AGG'CCT
Cuts at: 358 832 856 358
Size: 474 24 5456
Fragments arranged by size:
5456 474 24
StyI C'CwwG_G
Cuts at: 281 332 359 503 851 935 1637 2444 3088
Size: 51 27 144 348 84 702 807 644
Cuts at: 3088 3442 281
Size: 354 2793
Fragments arranged by size:
2793 807 702 644 354 348 144 84
51 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 732 885 954 2621 3537 3670 4129 4338 732
Size: 153 69 1667 916 133 459 209 2348
Fragments arranged by size:
2348 1667 916 459 209 153 133 69
TatI w'GTAC_w
Cuts at: 1523 2297 2483 5469 1523
Size: 774 186 2986 2008
Fragments arranged by size:
2986 2008 774 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 118 150 1143 1175 2425 2457 5471 5503 118
Size: 32 993 32 1250 32 3014 32 569
Fragments arranged by size:
3014 1250 993 569 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3751 3751
Size: 5954
XbaI T'CTAG_A
Cuts at: 2985 2985
Size: 5954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3449 3449
Size: 5954
XhoI C'TCGA_G
Cuts at: 1966 2854 1966
Size: 888 5066
XmaI C'CCGG_G
Cuts at: 638 682 2210 2974 638
Size: 44 1528 764 3618
Fragments arranged by size:
3618 1528 764 44
XmnI GAAnn'nnTTC
Cuts at: 2311 2311
Size: 5954
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PspOMI SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI ZraI