Restriction map for
pF1KA0569
(Circular) (Six-base) MAPSORT of: pF1KA0569.seq Check: 5290 from: 1 to: 6755
pF1KA0569 6755 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1016 1016
Size: 6755
AccI GT'mk_AC
Cuts at: 2198 3793 2198
Size: 1595 5160
Acc65I G'GTAC_C
Cuts at: 3771 3771
Size: 6755
AclI AA'CG_TT
Cuts at: 1059 1908 6595 1059
Size: 849 4687 1219
Fragments arranged by size:
4687 1219 849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 805 1568 4580 5012 5365 6027 592
Size: 213 763 3012 432 353 662 1320
Fragments arranged by size:
3012 1320 763 662 432 353 213
AfeI AGC'GCT
Cuts at: 772 3295 772
Size: 2523 4232
AflIII A'CryG_T
Cuts at: 324 851 2072 3023 4107 5907 6188 324
Size: 527 1221 951 1084 1800 281 891
Fragments arranged by size:
1800 1221 1084 951 891 527 281
AhdI GACnn_n'nnGTC
Cuts at: 2073 3024 2073
Size: 951 5804
AleI CACnn'nnGTG
Cuts at: 1022 1424 2354 3189 1022
Size: 402 930 835 4588
Fragments arranged by size:
4588 930 835 402
AlwNI CAG_nnn'CTG
Cuts at: 214 4060 5498 214
Size: 3846 1438 1471
Fragments arranged by size:
3846 1471 1438
ApaLI G'TGCA_C
Cuts at: 5593 5593
Size: 6755
ApoI r'AATT_y
Cuts at: 1013 1553 2135 2164 2211 2710 2718 3759 6108
Size: 540 582 29 47 499 8 1041 2349
Cuts at: 6108 6364 1013
Size: 256 1404
Fragments arranged by size:
2349 1404 1041 582 540 499 256 47
29 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 6755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6755
AvaI C'yCGr_G
Cuts at: 874 3255 3306 3775 874
Size: 2381 51 469 3854
Fragments arranged by size:
3854 2381 469 51
BamHI G'GATC_C
Cuts at: 1584 3630 3780 1584
Size: 2046 150 4559
Fragments arranged by size:
4559 2046 150
BanI G'GyrC_C
Cuts at: 1666 3771 4185 4433 4468 1666
Size: 2105 414 248 35 3953
Fragments arranged by size:
3953 2105 414 248 35
BanII G_rGCy'C
Cuts at: 2114 2540 3313 3769 4799 2114
Size: 426 773 456 1030 4070
Fragments arranged by size:
4070 1030 773 456 426
BbeI G_GCGC'C
Cuts at: 4189 4437 4189
Size: 248 6507
BbsI GAAGACnn'nnnn_
Cuts at: 236 458 1636 3091 3734 236
Size: 222 1178 1455 643 3257
Fragments arranged by size:
3257 1455 1178 643 222
BbvCI CC'TCA_GC
Cuts at: 248 248
Size: 6755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 565 599 855 889 565
Size: 34 256 34 6431
Fragments arranged by size:
6431 256 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3618 4653 5709 6755 3618
Size: 1035 1056 1046 3618
Fragments arranged by size:
3618 1056 1046 1035
BclI T'GATC_A
Cuts at: 4275 4275
Size: 6755
BfrBI ATG'CAT
Cuts at: 3106 3106
Size: 6755
BglII A'GATC_T
Cuts at: 4270 6307 4270
Size: 2037 4718
BlpI GC'TnA_GC
Cuts at: 2546 3867 2546
Size: 1321 5434
Bme1580I G_kGCm'C
Cuts at: 305 1045 3407 4380 4473 5597 305
Size: 740 2362 973 93 1124 1463
Fragments arranged by size:
2362 1463 1124 973 740 93
BmrI ACTGGGnnnn_n'
Cuts at: 1203 2746 3967 4048 4383 6527 1203
Size: 1543 1221 81 335 2144 1431
Fragments arranged by size:
2144 1543 1431 1221 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 353 2133 2468 5122 353
Size: 1780 335 2654 1986
Fragments arranged by size:
2654 1986 1780 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 701 1711 2424 2524 2951 3683 586
Size: 115 1010 713 100 427 732 3658
Fragments arranged by size:
3658 1010 732 713 427 115 100
Bpu10I CC'TnA_GC
Cuts at: 248 248
Size: 6755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1731 3228 3931 4810 5524 5822 1731
Size: 1497 703 879 714 298 2664
Fragments arranged by size:
2664 1497 879 714 703 298
BsaI GGTCTCn'nnnn_
Cuts at: 2303 6459 2303
Size: 4156 2599
BsaAI yAC'GTr
Cuts at: 327 1786 4108 327
Size: 1459 2322 2974
Fragments arranged by size:
2974 2322 1459
BsaBI GATnn'nnATC
Cuts at: 6 3629 4269 6306 6
Size: 3623 640 2037 455
Fragments arranged by size:
3623 2037 640 455
BsaHI Gr'CG_yC
Cuts at: 4186 4434 5136 6632 4186
Size: 248 702 1496 4309
Fragments arranged by size:
4309 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1647 3944 4465 5143 5554 5701 5975 1647
Size: 2297 521 678 411 147 274 2427
Fragments arranged by size:
2427 2297 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1976 2006 2442 2472 3040 3070 1976
Size: 30 436 30 568 30 5661
Fragments arranged by size:
5661 568 436 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 613 664 1999 2366 2548 3379 3496 3595 3604
Size: 51 1335 367 182 831 117 99 9
Cuts at: 3604 613
Size: 3764
Fragments arranged by size:
3764 1335 831 367 182 117 99 51
9
BseYI C'CCAG_C
Cuts at: 869 3558 4180 4938 5603 6011 869
Size: 2689 622 758 665 408 1613
Fragments arranged by size:
2689 1613 758 665 622 408
BsiEI CG_ry'CG
Cuts at: 104 759 4297 4343 5573 104
Size: 655 3538 46 1230 1286
Fragments arranged by size:
3538 1286 1230 655 46
BsiHKAI G_wGCw'C
Cuts at: 794 2540 3015 3242 3527 3769 4547 4737 5597
Size: 1746 475 227 285 242 778 190 860
Cuts at: 5597 794
Size: 1952
Fragments arranged by size:
1952 1746 860 778 475 285 242 227
190
BsmI GAATG_Cn'
Cuts at: 522 522
Size: 6755
BsmBI CGTCTCn'nnnn_
Cuts at: 277 277
Size: 6755
Bsp1286I G_dGCh'C
Cuts at: 305 794 1045 2114 2540 3015 3242 3313 3407
Size: 489 251 1069 426 475 227 71 94
Cuts at: 3407 3527 3769 4380 4473 4547 4737 4799 5597
Size: 120 242 611 93 74 190 62 798
Cuts at: 5597 305
Size: 1463
Fragments arranged by size:
1463 1069 798 611 489 475 426 251
242 227 190 120 94 93 74 71
62
BspHI T'CATG_A
Cuts at: 1956 5187 1956
Size: 3231 3524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 710 751 928 1016 3805 4019 4321 4702 710
Size: 41 177 88 2789 214 302 381 2763
Fragments arranged by size:
2789 2763 381 302 214 177 88 41
BsrBI CCG'CTC
Cuts at: 50 879 3369 5047 5101 6585 6750 50
Size: 829 2490 1678 54 1484 165 55
Fragments arranged by size:
2490 1678 1484 829 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 4667 2141
Size: 2526 4229
BsrFI r'CCGG_y
Cuts at: 4753 4934 4753
Size: 181 6574
BssHII G'CGCG_C
Cuts at: 3391 3391
Size: 6755
BssSI C'ACGA_G
Cuts at: 808 3526 3721 5026 5734 808
Size: 2718 195 1305 708 1829
Fragments arranged by size:
2718 1829 1305 708 195
BstAPI GCAn_nnn'nTGC
Cuts at: 921 2095 4132 921
Size: 1174 2037 3544
Fragments arranged by size:
3544 2037 1174
BstBI TT'CG_AA
Cuts at: 5116 5116
Size: 6755
BstXI CCAn_nnnn'nTGG
Cuts at: 273 2148 273
Size: 1875 4880
BstYI r'GATC_y
Cuts at: 1 1584 3630 3780 4247 4270 4605 4851 5157
Size: 1583 2046 150 467 23 335 246 306
Cuts at: 5157 5169 5255 6307 1
Size: 12 86 1052 449
Fragments arranged by size:
2046 1583 1052 467 449 335 306 246
150 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 647 1601 2159 3461 647
Size: 954 558 1302 3941
Fragments arranged by size:
3941 1302 954 558
BtgI C'CryG_G
Cuts at: 3674 3674
Size: 6755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 590 4705 4886 5049 590
Size: 4115 181 163 2296
Fragments arranged by size:
4115 2296 181 163
BtsI GCAGTG_nn'
Cuts at: 621 3998 621
Size: 3377 3378
DraI TTT'AAA
Cuts at: 3754 3754
Size: 6755
DraIII CAC_nnn'GTG
Cuts at: 965 965
Size: 6755
DrdI GACnn_nn'nnGTC
Cuts at: 4461 5805 4461
Size: 1344 5411
EaeI y'GGCC_r
Cuts at: 1131 4340 4514 4905 4932 6277 1131
Size: 3209 174 391 27 1345 1609
Fragments arranged by size:
3209 1609 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4340 4340
Size: 6755
EarI CTCTTCn'nnn_
Cuts at: 350 815 1925 2532 2804 3590 3650 4778 4988
Size: 465 1110 607 272 786 60 1128 210
Cuts at: 4988 350
Size: 2117
Fragments arranged by size:
2117 1128 1110 786 607 465 272 210
60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 1622 3376 3394 4963 5705 5851 6565 142
Size: 1480 1754 18 1569 742 146 714 332
Fragments arranged by size:
1754 1569 1480 742 714 332 146 18
EcoICRI GAG'CTC
Cuts at: 2538 3767 2538
Size: 1229 5526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 592 701 805 1568 1711 2424 2524 2951
Size: 6 109 104 763 143 713 100 427
Cuts at: 2951 3683 4580 5012 5365 6027 586
Size: 732 897 432 353 662 1314
Fragments arranged by size:
1314 897 763 732 713 662 432 427
353 143 109 104 100 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2158 2158
Size: 6755
EcoO109I rG'GnC_Cy
Cuts at: 2219 3894 2219
Size: 1675 5080
EcoRI G'AATT_C
Cuts at: 1013 3759 1013
Size: 2746 4009
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 253 4774 253
Size: 4521 2234
FspI TGC'GCA
Cuts at: 4536 4536
Size: 6755
HaeII r_GCGC'y
Cuts at: 774 3297 4189 4437 5667 6438 774
Size: 2523 892 248 1230 771 1091
Fragments arranged by size:
2523 1230 1091 892 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 640 672 1576 1608 2059 2091 3010 3040 3042
Size: 32 904 32 451 32 919 30 2
Cuts at: 3042 3072 3651 3683 4597 4627 4629 4659 640
Size: 30 579 32 914 30 2 30 2736
Fragments arranged by size:
2736 919 914 904 579 451 32 32
32 32 30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 3794 3794
Size: 6755
HindIII A'AGCT_T
Cuts at: 235 1718 235
Size: 1483 5272
KasI G'GCGC_C
Cuts at: 4185 4433 4185
Size: 248 6507
KpnI G_GTAC'C
Cuts at: 3775 3775
Size: 6755
MluI A'CGCG_T
Cuts at: 6188 6188
Size: 6755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1676 3077 5514 5698 1205
Size: 471 1401 2437 184 2262
Fragments arranged by size:
2437 2262 1401 471 184
MscI TGG'CCA
Cuts at: 4516 4516
Size: 6755
MslI CAynn'nnrTG
Cuts at: 1022 1424 1664 1851 1901 2146 2354 2648 3189
Size: 402 240 187 50 245 208 294 541
Cuts at: 3189 4744 4871 1022
Size: 1555 127 2906
Fragments arranged by size:
2906 1555 541 402 294 245 240 208
187 127 50
MspA1I CmG'CkG
Cuts at: 2976 3866 4180 4540 5324 5569 2976
Size: 890 314 360 784 245 4162
Fragments arranged by size:
4162 890 784 360 314 245
NaeI GCC'GGC
Cuts at: 4936 4936
Size: 6755
NarI GG'CG_CC
Cuts at: 4186 4434 4186
Size: 248 6507
NcoI C'CATG_G
Cuts at: 3674 3674
Size: 6755
NdeI CA'TA_TG
Cuts at: 884 2909 884
Size: 2025 4730
NgoMIV G'CCGG_C
Cuts at: 4934 4934
Size: 6755
NsiI A_TGCA'T
Cuts at: 3108 3108
Size: 6755
NspI r_CATG'y
Cuts at: 2076 3027 3102 3110 3808 4308 5911 2076
Size: 951 75 8 698 500 1603 2920
Fragments arranged by size:
2920 1603 951 698 500 75 8
PciI A'CATG_T
Cuts at: 2072 3023 5907 2072
Size: 951 2884 2920
Fragments arranged by size:
2920 2884 951
PfoI T'CCnGG_A
Cuts at: 2440 2440
Size: 6755
PmeI GTTT'AAAC
Cuts at: 3754 3754
Size: 6755
PmlI CAC'GTG
Cuts at: 327 327
Size: 6755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5201 5233 5201
Size: 32 6723
PpuMI rG'GwC_Cy
Cuts at: 2219 2219
Size: 6755
PshAI GACnn'nnGTC
Cuts at: 3059 3059
Size: 6755
PspXI vC'TCGA_Gb
Cuts at: 874 874
Size: 6755
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1478 1510 3424 3456 1478
Size: 32 1914 32 4777
Fragments arranged by size:
4777 1914 32 32
PstI C_TGCA'G
Cuts at: 1952 3802 4487 1952
Size: 1850 685 4220
Fragments arranged by size:
4220 1850 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6755
PvuII CAG'CTG
Cuts at: 4180 4540 4180
Size: 360 6395
RsrII CG'GwC_CG
Cuts at: 4950 4950
Size: 6755
SacI G_AGCT'C
Cuts at: 2540 3769 2540
Size: 1229 5526
SalI G'TCGA_C
Cuts at: 3792 3792
Size: 6755
SapI GCTCTTCn'nnn_
Cuts at: 2804 4778 4988 2804
Size: 1974 210 4571
Fragments arranged by size:
4571 1974 210
SbfI CC_TGCA'GG
Cuts at: 3802 3802
Size: 6755
ScaI AGT'ACT
Cuts at: 2206 6272 2206
Size: 4066 2689
SfcI C'TryA_G
Cuts at: 33 425 843 1080 1498 1948 2336 3798 4483
Size: 392 418 237 418 450 388 1462 685
Cuts at: 4483 5451 5642 33
Size: 968 191 1146
Fragments arranged by size:
1462 1146 968 685 450 418 418 392
388 237 191
SfoI GGC'GCC
Cuts at: 4187 4435 4187
Size: 248 6507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6755
SmaI CCC'GGG
Cuts at: 3777 3777
Size: 6755
SmlI C'TyrA_G
Cuts at: 874 1746 3243 3910 4825 5539 5801 874
Size: 872 1497 667 915 714 262 1828
Fragments arranged by size:
1828 1497 915 872 714 667 262
SnaBI TAC'GTA
Cuts at: 1786 1786
Size: 6755
SpeI A'CTAG_T
Cuts at: 67 1342 67
Size: 1275 5480
SphI G_CATG'C
Cuts at: 3808 4308 3808
Size: 500 6255
SspI AAT'ATT
Cuts at: 1629 2412 1629
Size: 783 5972
StuI AGG'CCT
Cuts at: 976 3032 976
Size: 2056 4699
StyI C'CwwG_G
Cuts at: 663 930 3674 3889 4243 663
Size: 267 2744 215 354 3175
Fragments arranged by size:
3175 2744 354 267 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 883 1630 4338 4471 4930 5139 883
Size: 747 2708 133 459 209 2499
Fragments arranged by size:
2708 2499 747 459 209 133
TatI w'GTAC_w
Cuts at: 2022 2079 2204 6270 2022
Size: 57 125 4066 2507
Fragments arranged by size:
4066 2507 125 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1822 1854 2784 2816 3325 3357 6272 6304 1822
Size: 32 930 32 509 32 2915 32 2273
Fragments arranged by size:
2915 2273 930 509 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4552 4552
Size: 6755
XbaI T'CTAG_A
Cuts at: 3786 3786
Size: 6755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 561 1530 4250 561
Size: 969 2720 3066
Fragments arranged by size:
3066 2720 969
XhoI C'TCGA_G
Cuts at: 874 874
Size: 6755
XmaI C'CCGG_G
Cuts at: 3775 3775
Size: 6755
XmnI GAAnn'nnTTC
Cuts at: 1060 1874 3211 1060
Size: 814 1337 4604
Fragments arranged by size:
4604 1337 814
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI ApaI AscI AvrII
BaeI BglI BmgBI BmtI BsgI BsiWI BspEI BsrGI
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI PacI PasI PflMI PsiI
PspOMI SacII SanDI SexAI SfiI SgrAI SrfI SwaI
ZraI