Restriction map for
pF1KA0563
(Circular) (Six-base) MAPSORT of: pF1KA0563.seq Check: 5863 from: 1 to: 8015
pF1KA0563 8015 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 3800 4110 5053 3800
Size: 310 943 6762
Fragments arranged by size:
6762 943 310
Acc65I G'GTAC_C
Cuts at: 5031 5031
Size: 8015
AclI AA'CG_TT
Cuts at: 7855 7855
Size: 8015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 1019 2198 2492 3302 4226 5840 6272 7019
Size: 442 1179 294 810 924 1614 432 747
Cuts at: 7019 7287 577
Size: 268 1305
Fragments arranged by size:
1614 1305 1179 924 810 747 442 432
294 268
AflII C'TTAA_G
Cuts at: 3121 4191 3121
Size: 1070 6945
AflIII A'CryG_T
Cuts at: 5367 6471 7448 5367
Size: 1104 977 5934
Fragments arranged by size:
5934 1104 977
AgeI A'CCGG_T
Cuts at: 6403 6403
Size: 8015
AhdI GACnn_n'nnGTC
Cuts at: 807 1256 807
Size: 449 7566
AleI CACnn'nnGTG
Cuts at: 135 135
Size: 8015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1616 1616
Size: 8015
AlwNI CAG_nnn'CTG
Cuts at: 225 2395 2454 3597 3844 4548 5320 6887 225
Size: 2170 59 1143 247 704 772 1567 1353
Fragments arranged by size:
2170 1567 1353 1143 772 704 247 59
ApaI G_GGCC'C
Cuts at: 169 374 734 1483 3619 4632 169
Size: 205 360 749 2136 1013 3552
Fragments arranged by size:
3552 2136 1013 749 360 205
ApaLI G'TGCA_C
Cuts at: 6785 6785
Size: 8015
ApoI r'AATT_y
Cuts at: 420 2718 4161 4529 5019 7368 7624 420
Size: 2298 1443 368 490 2349 256 811
Fragments arranged by size:
2349 2298 1443 811 490 368 256
AscI GG'CGCG_CC
Cuts at: 3869 3869
Size: 8015
AseI AT'TA_AT
Cuts at: 20 1565 4763 20
Size: 1545 3198 3272
Fragments arranged by size:
3272 3198 1545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8015
AvaI C'yCGr_G
Cuts at: 2084 4474 5035 2084
Size: 2390 561 5064
Fragments arranged by size:
5064 2390 561
BamHI G'GATC_C
Cuts at: 5040 5040
Size: 8015
BanI G'GyrC_C
Cuts at: 2701 3682 5031 5445 5693 5728 2701
Size: 981 1349 414 248 35 4988
Fragments arranged by size:
4988 1349 981 414 248 35
BanII G_rGCy'C
Cuts at: 169 284 374 734 773 1483 2606 2687 3383
Size: 115 90 360 39 710 1123 81 696
Cuts at: 3383 3619 4481 4632 5029 6059 169
Size: 236 862 151 397 1030 2125
Fragments arranged by size:
2125 1123 1030 862 710 696 397 360
236 151 115 90 81 39
BbeI G_GCGC'C
Cuts at: 5449 5697 5449
Size: 248 7767
BbsI GAAGACnn'nnnn_
Cuts at: 281 806 1205 1631 2425 3991 4753 281
Size: 525 399 426 794 1566 762 3543
Fragments arranged by size:
3543 1566 794 762 525 426 399
BbvCI CC'TCA_GC
Cuts at: 1625 1625
Size: 8015
BciVI GTATCCnnnnn_n'
Cuts at: 689 4725 5913 6674 8015 689
Size: 4036 1188 761 1341 689
Fragments arranged by size:
4036 1341 1188 761 689
BclI T'GATC_A
Cuts at: 360 4518 5535 360
Size: 4158 1017 2840
Fragments arranged by size:
4158 2840 1017
BglI GCCn_nnn'nGGC
Cuts at: 729 729
Size: 8015
BglII A'GATC_T
Cuts at: 4916 5530 7567 4916
Size: 614 2037 5364
Fragments arranged by size:
5364 2037 614
BlpI GC'TnA_GC
Cuts at: 218 572 1607 1901 2231 5127 218
Size: 354 1035 294 330 2896 3106
Fragments arranged by size:
3106 2896 1035 354 330 294
Bme1580I G_kGCm'C
Cuts at: 127 169 374 734 1483 3619 3687 4462 4632
Size: 42 205 360 749 2136 68 775 170
Cuts at: 4632 5640 5733 6789 127
Size: 1008 93 1056 1353
Fragments arranged by size:
2136 1353 1056 1008 775 749 360 205
170 93 68 42
BmgBI CAC'GTC
Cuts at: 1356 1356
Size: 8015
BmrI ACTGGGnnnn_n'
Cuts at: 1325 5227 5308 5643 7787 1325
Size: 3902 81 335 2144 1553
Fragments arranged by size:
3902 2144 1553 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 944 1596 2355 3399 6382 944
Size: 652 759 1044 2983 2577
Fragments arranged by size:
2983 2577 1044 759 652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 247 476 490 680 710 805 887 944
Size: 15 229 14 190 30 95 82 57
Cuts at: 944 1130 1250 1280 1334 1658 1691 1754 1946
Size: 186 120 30 54 324 33 63 192
Cuts at: 1946 2168 2296 2434 2836 3867 4647 232
Size: 222 128 138 402 1031 780 3600
Fragments arranged by size:
3600 1031 780 402 324 229 222 192
190 186 138 128 120 95 82 63
57 54 33 30 30 15 14
Bpu10I CC'TnA_GC
Cuts at: 278 1625 2507 278
Size: 1347 882 5786
Fragments arranged by size:
5786 1347 882
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1712 2234 4193 4828 4947 5191 6070 6562
Size: 835 522 1959 635 119 244 879 492
Cuts at: 6562 6860 877
Size: 298 2032
Fragments arranged by size:
2032 1959 879 835 635 522 492 298
244 119
BsaI GGTCTCn'nnnn_
Cuts at: 784 1072 1243 1807 2329 4190 7719 784
Size: 288 171 564 522 1861 3529 1080
Fragments arranged by size:
3529 1861 1080 564 522 288 171
BsaAI yAC'GTr
Cuts at: 5368 5368
Size: 8015
BsaBI GATnn'nnATC
Cuts at: 6 3053 5529 7566 6
Size: 3047 2476 2037 455
Fragments arranged by size:
3047 2476 2037 455
BsaHI Gr'CG_yC
Cuts at: 5446 5694 6396 7892 5446
Size: 248 702 1496 5569
Fragments arranged by size:
5569 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1554 4601 5204 5725 6403 6677 6824 7235 1554
Size: 3047 603 521 678 274 147 411 2334
Fragments arranged by size:
3047 2334 678 603 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1621 1651 1752 1782 1812 1842 1944 1974 2109
Size: 30 101 30 30 30 102 30 135
Cuts at: 2109 2139 2160 2163 2190 2193 2412 2442 2563
Size: 30 21 3 27 3 219 30 121
Cuts at: 2563 2593 1621
Size: 30 7043
Fragments arranged by size:
7043 219 135 121 102 101 30 30
30 30 30 30 30 30 27 21
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 288 1114 1642 2168 2171 2347 2420 3391 5002
Size: 826 528 526 3 176 73 971 1611
Cuts at: 5002 5005 288
Size: 3 3298
Fragments arranged by size:
3298 1611 971 826 528 526 176 73
3 3
BseYI C'CCAG_C
Cuts at: 339 834 891 1070 1241 1589 1662 1918 4390
Size: 495 57 179 171 348 73 256 2472
Cuts at: 4390 4541 5440 6198 6775 7271 339
Size: 151 899 758 577 496 1083
Fragments arranged by size:
2472 1083 899 758 577 496 495 348
256 179 171 151 73 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1378 1406 2542 2543 1378
Size: 28 1136 1 6850
Fragments arranged by size:
6850 1136 28 1
BsiEI CG_ry'CG
Cuts at: 104 5557 5603 6811 104
Size: 5453 46 1208 1308
Fragments arranged by size:
5453 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2606 4774 5029 5807 5997 6789 2606
Size: 2168 255 778 190 792 3832
Fragments arranged by size:
3832 2168 792 778 255 190
BsmI GAATG_Cn'
Cuts at: 1713 2070 2235 2999 4399 1713
Size: 357 165 764 1400 5329
Fragments arranged by size:
5329 1400 764 357 165
BsmBI CGTCTCn'nnnn_
Cuts at: 4665 4665
Size: 8015
Bsp1286I G_dGCh'C
Cuts at: 127 169 284 374 734 773 1483 2606 2687
Size: 42 115 90 360 39 710 1123 81
Cuts at: 2687 3383 3619 3687 4462 4481 4632 4774 5029
Size: 696 236 68 775 19 151 142 255
Cuts at: 5029 5640 5733 5807 5997 6059 6789 127
Size: 611 93 74 190 62 730 1353
Fragments arranged by size:
1353 1123 775 730 710 696 611 360
255 236 190 151 142 115 93 90
81 74 68 62 42 39 19
BspEI T'CCGG_A
Cuts at: 1554 4601 7235 1554
Size: 3047 2634 2334
Fragments arranged by size:
3047 2634 2334
BspHI T'CATG_A
Cuts at: 939 1275 3134 3317 7191 939
Size: 336 1859 183 3874 1763
Fragments arranged by size:
3874 1859 1763 336 183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1017 2511 2620 5065 5279 5581 5962 1017
Size: 1494 109 2445 214 302 381 3070
Fragments arranged by size:
3070 2445 1494 381 302 214 109
BsrBI CCG'CTC
Cuts at: 50 118 246 6307 6361 7845 8010 50
Size: 68 128 6061 54 1484 165 55
Fragments arranged by size:
6061 1484 165 128 68 55 54
BsrDI GCAATG_nn'
Cuts at: 2952 3240 4498 4586 5927 2952
Size: 288 1258 88 1341 5040
Fragments arranged by size:
5040 1341 1258 288 88
BsrFI r'CCGG_y
Cuts at: 3587 6013 6194 6403 3587
Size: 2426 181 209 5199
Fragments arranged by size:
5199 2426 209 181
BssHII G'CGCG_C
Cuts at: 3869 3869
Size: 8015
BssSI C'ACGA_G
Cuts at: 6286 6644 6286
Size: 358 7657
BstAPI GCAn_nnn'nTGC
Cuts at: 2994 4291 5392 2994
Size: 1297 1101 5617
Fragments arranged by size:
5617 1297 1101
BstBI TT'CG_AA
Cuts at: 6376 6376
Size: 8015
BstEII G'GTnAC_C
Cuts at: 1023 1300 1023
Size: 277 7738
BstYI r'GATC_y
Cuts at: 1 564 1749 3048 4916 5040 5507 5530 5865
Size: 563 1185 1299 1868 124 467 23 335
Cuts at: 5865 6111 7123 7209 7221 7567 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1868 1299 1185 1012 563 467 449 346
335 246 124 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2030 2195 4376 2030
Size: 165 2181 5669
Fragments arranged by size:
5669 2181 165
BtgI C'CryG_G
Cuts at: 169 310 349 2327 169
Size: 141 39 1978 5857
Fragments arranged by size:
5857 1978 141 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5965 6146 6309 5965
Size: 181 163 7671
Fragments arranged by size:
7671 181 163
BtsI GCAGTG_nn'
Cuts at: 3399 3704 5258 3399
Size: 305 1554 6156
Fragments arranged by size:
6156 1554 305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 178 213 178
Size: 35 7980
DraI TTT'AAA
Cuts at: 506 3063 3201 5014 506
Size: 2557 138 1813 3507
Fragments arranged by size:
3507 2557 1813 138
DraIII CAC_nnn'GTG
Cuts at: 1333 1333
Size: 8015
DrdI GACnn_nn'nnGTC
Cuts at: 5721 6579 5721
Size: 858 7157
EaeI y'GGCC_r
Cuts at: 5600 5774 6165 6192 7537 5600
Size: 174 391 27 1345 6078
Fragments arranged by size:
6078 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5600 5600
Size: 8015
EarI CTCTTCn'nnn_
Cuts at: 819 1996 4144 6038 6248 819
Size: 1177 2148 1894 210 2586
Fragments arranged by size:
2586 2148 1894 1177 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2653 6223 6533 6679 7825 2653
Size: 3570 310 146 1146 2843
Fragments arranged by size:
3570 2843 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2604 5027 2604
Size: 2423 5592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 247 476 490 577 680 710 805 887
Size: 15 229 14 87 103 30 95 82
Cuts at: 887 944 1019 1130 1250 1280 1334 1658 1691
Size: 57 75 111 120 30 54 324 33
Cuts at: 1691 1754 1946 2168 2198 2296 2434 2492 2836
Size: 63 192 222 30 98 138 58 344
Cuts at: 2836 3302 3867 4226 4647 5840 6272 7019 7287
Size: 466 565 359 421 1193 432 747 268
Cuts at: 7287 232
Size: 960
Fragments arranged by size:
1193 960 747 565 466 432 421 359
344 324 268 229 222 192 138 120
111 103 98 95 87 82 75 63
58 57 54 33 30 30 30 15
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2183 3904 4172 2183
Size: 1721 268 6026
Fragments arranged by size:
6026 1721 268
EcoO109I rG'GnC_Cy
Cuts at: 165 240 370 371 730 731 888 1131 1479
Size: 75 130 1 359 1 157 243 348
Cuts at: 1479 1659 3772 4628 5154 165
Size: 180 2113 856 526 3026
Fragments arranged by size:
3026 2113 856 526 359 348 243 180
157 130 75 1 1
EcoRI G'AATT_C
Cuts at: 5019 5019
Size: 8015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6034 6034
Size: 8015
FspI TGC'GCA
Cuts at: 5796 5796
Size: 8015
HaeII r_GCGC'y
Cuts at: 5449 5697 6719 7698 5449
Size: 248 1022 979 5766
Fragments arranged by size:
5766 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 271 303 793 825 1042 1074 1213
Size: 32 137 32 490 32 217 32 139
Cuts at: 1213 1242 1245 1274 1621 1653 1806 1838 1940
Size: 29 3 29 347 32 153 32 102
Cuts at: 1940 1972 2160 2192 2328 2354 2360 2386 2412
Size: 32 188 32 136 26 6 26 26
Cuts at: 2412 2442 2444 2474 2614 2646 3496 3528 4103
Size: 30 2 30 140 32 850 32 575
Cuts at: 4103 4135 4908 4940 5857 5887 5889 5919 102
Size: 32 773 32 917 30 2 30 2198
Fragments arranged by size:
2198 917 850 773 575 490 347 217
188 153 140 139 137 136 102 32
32 32 32 32 32 32 32 32
32 32 32 30 30 30 30 29
29 26 26 26 6 3 2 2
HincII GTy'rAC
Cuts at: 3498 5054 3498
Size: 1556 6459
HindIII A'AGCT_T
Cuts at: 974 4485 4684 974
Size: 3511 199 4305
Fragments arranged by size:
4305 3511 199
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 8015
KasI G'GCGC_C
Cuts at: 5445 5693 5445
Size: 248 7767
KpnI G_GTAC'C
Cuts at: 5035 5035
Size: 8015
MfeI C'AATT_G
Cuts at: 1136 1136
Size: 8015
MluI A'CGCG_T
Cuts at: 7448 7448
Size: 8015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 559 726 1261 1609 1825 1903 1962 2347 2755
Size: 167 535 348 216 78 59 385 408
Cuts at: 2755 6686 6870 559
Size: 3931 184 1704
Fragments arranged by size:
3931 1704 535 408 385 348 216 184
167 78 59
MscI TGG'CCA
Cuts at: 5776 5776
Size: 8015
MslI CAynn'nnrTG
Cuts at: 135 3133 3322 4327 6004 6131 135
Size: 2998 189 1005 1677 127 2019
Fragments arranged by size:
2998 2019 1677 1005 189 127
MspA1I CmG'CkG
Cuts at: 153 252 454 1606 1624 4390 4548 5126 5440
Size: 99 202 1152 18 2766 158 578 314
Cuts at: 5440 5800 6813 7058 153
Size: 360 1013 245 1110
Fragments arranged by size:
2766 1152 1110 1013 578 360 314 245
202 158 99 18
NaeI GCC'GGC
Cuts at: 6196 6196
Size: 8015
NarI GG'CG_CC
Cuts at: 5446 5694 5446
Size: 248 7767
NcoI C'CATG_G
Cuts at: 169 169
Size: 8015
NdeI CA'TA_TG
Cuts at: 2596 3176 2596
Size: 580 7435
NgoMIV G'CCGG_C
Cuts at: 6194 6194
Size: 8015
NspI r_CATG'y
Cuts at: 3257 4332 5068 5568 6475 3257
Size: 1075 736 500 907 4797
Fragments arranged by size:
4797 1075 907 736 500
PasI CC'CwG_GG
Cuts at: 267 267
Size: 8015
PciI A'CATG_T
Cuts at: 6471 6471
Size: 8015
PflMI CCAn_nnn'nTGG
Cuts at: 4834 4834
Size: 8015
PfoI T'CCnGG_A
Cuts at: 434 2184 4663 4827 434
Size: 1750 2479 164 3622
Fragments arranged by size:
3622 2479 1750 164
PmeI GTTT'AAAC
Cuts at: 5014 5014
Size: 8015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2078 2110 7154 7186 2078
Size: 32 5044 32 2907
Fragments arranged by size:
5044 2907 32 32
PpuMI rG'GwC_Cy
Cuts at: 240 240
Size: 8015
PshAI GACnn'nnGTC
Cuts at: 2222 2473 4202 2222
Size: 251 1729 6035
Fragments arranged by size:
6035 1729 251
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 8015
PspOMI G'GGCC_C
Cuts at: 165 370 730 1479 3615 4628 165
Size: 205 360 749 2136 1013 3552
Fragments arranged by size:
3552 2136 1013 749 360 205
PspXI vC'TCGA_Gb
Cuts at: 2084 2084
Size: 8015
PstI C_TGCA'G
Cuts at: 1014 1605 4146 4553 5062 5747 1014
Size: 591 2541 407 509 685 3282
Fragments arranged by size:
3282 2541 685 591 509 407
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8015
PvuII CAG'CTG
Cuts at: 252 454 1606 4548 5440 5800 252
Size: 202 1152 2942 892 360 2467
Fragments arranged by size:
2942 2467 1152 892 360 202
RsrII CG'GwC_CG
Cuts at: 6210 6210
Size: 8015
SacI G_AGCT'C
Cuts at: 2606 5029 2606
Size: 2423 5592
SalI G'TCGA_C
Cuts at: 5052 5052
Size: 8015
SapI GCTCTTCn'nnn_
Cuts at: 4144 6038 6248 4144
Size: 1894 210 5911
Fragments arranged by size:
5911 1894 210
SbfI CC_TGCA'GG
Cuts at: 5062 5062
Size: 8015
ScaI AGT'ACT
Cuts at: 7532 7532
Size: 8015
SexAI A'CCwGG_T
Cuts at: 1884 2054 1884
Size: 170 7845
SfcI C'TryA_G
Cuts at: 33 1010 1601 2009 2156 2171 2303 2387 2405
Size: 977 591 408 147 15 132 84 18
Cuts at: 2405 2423 3293 3575 4142 4549 5058 5743 6736
Size: 18 870 282 567 407 509 685 993
Cuts at: 6736 6927 33
Size: 191 1121
Fragments arranged by size:
1121 993 977 870 685 591 567 509
408 407 282 191 147 132 84 18
18 15
SfoI GGC'GCC
Cuts at: 5447 5695 5447
Size: 248 7767
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8015
SmaI CCC'GGG
Cuts at: 5037 5037
Size: 8015
SmlI C'TyrA_G
Cuts at: 856 1727 2084 2249 3121 4191 4208 4807 4962
Size: 871 357 165 872 1070 17 599 155
Cuts at: 4962 5170 6085 6577 6839 856
Size: 208 915 492 262 2032
Fragments arranged by size:
2032 1070 915 872 871 599 492 357
262 208 165 155 17
SpeI A'CTAG_T
Cuts at: 67 204 67
Size: 137 7878
SphI G_CATG'C
Cuts at: 5068 5568 5068
Size: 500 7515
SspI AAT'ATT
Cuts at: 658 658
Size: 8015
StuI AGG'CCT
Cuts at: 2578 2840 2983 2578
Size: 262 143 7610
Fragments arranged by size:
7610 262 143
StyI C'CwwG_G
Cuts at: 169 2724 3619 3739 3863 4064 5149 5503 169
Size: 2555 895 120 124 201 1085 354 2681
Fragments arranged by size:
2681 2555 1085 895 354 201 124 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1344 1932 2554 2707 2794 3835 4077 5598 5731
Size: 588 622 153 87 1041 242 1521 133
Cuts at: 5731 6190 6399 1344
Size: 459 209 2960
Fragments arranged by size:
2960 1521 1041 622 588 459 242 209
153 133 87
TatI w'GTAC_w
Cuts at: 664 3460 7530 664
Size: 2796 4070 1149
Fragments arranged by size:
4070 2796 1149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 601 633 2563 2595 3261 3293 3928 3960 7532
Size: 32 1930 32 666 32 635 32 3572
Cuts at: 7532 7564 601
Size: 32 1052
Fragments arranged by size:
3572 1930 1052 666 635 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5812 5812
Size: 8015
XbaI T'CTAG_A
Cuts at: 765 3084 5046 765
Size: 2319 1962 3734
Fragments arranged by size:
3734 2319 1962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 347 575 5510 347
Size: 228 4935 2852
Fragments arranged by size:
4935 2852 228
XhoI C'TCGA_G
Cuts at: 2084 2084
Size: 8015
XmaI C'CCGG_G
Cuts at: 5035 5035
Size: 8015
XmnI GAAnn'nnTTC
Cuts at: 3856 3856
Size: 8015
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AloI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsrGI BstXI BstZ17I ClaI EcoRV FseI
FspAI NheI NotI NruI NsiI PacI PmlI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI ZraI