Restriction map for
pF1KA0553
(Circular) (Six-base) MAPSORT of: pF1KA0553.seq Check: 8107 from: 1 to: 7385
pF1KA0553 7385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1779 1779
Size: 7385
AccI GT'mk_AC
Cuts at: 2526 4423 2526
Size: 1897 5488
Acc65I G'GTAC_C
Cuts at: 4401 4401
Size: 7385
AclI AA'CG_TT
Cuts at: 7225 7225
Size: 7385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 400 878 1760 2177 2489 3140 3440 5210
Size: 233 478 882 417 312 651 300 1770
Cuts at: 5210 5642 6389 6657 167
Size: 432 747 268 895
Fragments arranged by size:
1770 895 882 747 651 478 432 417
312 300 268 233
AfeI AGC'GCT
Cuts at: 2593 2731 2593
Size: 138 7247
AflIII A'CryG_T
Cuts at: 4737 5841 6818 4737
Size: 1104 977 5304
Fragments arranged by size:
5304 1104 977
AgeI A'CCGG_T
Cuts at: 5773 5773
Size: 7385
AleI CACnn'nnGTG
Cuts at: 2234 2234
Size: 7385
AlwNI CAG_nnn'CTG
Cuts at: 538 1045 1783 2053 3664 3879 3976 4036 4297
Size: 507 738 270 1611 215 97 60 261
Cuts at: 4297 4353 4690 6257 538
Size: 56 337 1567 1666
Fragments arranged by size:
1666 1611 1567 738 507 337 270 261
215 97 60 56
ApaI G_GGCC'C
Cuts at: 3236 3272 3416 4226 3236
Size: 36 144 810 6395
Fragments arranged by size:
6395 810 144 36
ApaLI G'TGCA_C
Cuts at: 497 6155 497
Size: 5658 1727
ApoI r'AATT_y
Cuts at: 322 628 4389 6738 6994 322
Size: 306 3761 2349 256 713
Fragments arranged by size:
3761 2349 713 306 256
AseI AT'TA_AT
Cuts at: 20 1540 20
Size: 1520 5865
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
AvaI C'yCGr_G
Cuts at: 2741 4405 2741
Size: 1664 5721
BamHI G'GATC_C
Cuts at: 3735 4410 3735
Size: 675 6710
BanI G'GyrC_C
Cuts at: 164 598 2272 2447 3547 4401 4815 5063 5098
Size: 434 1674 175 1100 854 414 248 35
Cuts at: 5098 164
Size: 2451
Fragments arranged by size:
2451 1674 1100 854 434 414 248 175
35
BanII G_rGCy'C
Cuts at: 1292 2124 2869 2917 3236 3272 3416 4226 4399
Size: 832 745 48 319 36 144 810 173
Cuts at: 4399 5429 1292
Size: 1030 3248
Fragments arranged by size:
3248 1030 832 810 745 319 173 144
48 36
BbeI G_GCGC'C
Cuts at: 168 2451 4819 5067 168
Size: 2283 2368 248 2486
Fragments arranged by size:
2486 2368 2283 248
BbsI GAAGACnn'nnnn_
Cuts at: 197 269 965 1847 3128 197
Size: 72 696 882 1281 4454
Fragments arranged by size:
4454 1281 882 696 72
BbvCI CC'TCA_GC
Cuts at: 1313 3764 3909 4206 1313
Size: 2451 145 297 4492
Fragments arranged by size:
4492 2451 297 145
BciVI GTATCCnnnnn_n'
Cuts at: 5283 6044 7385 5283
Size: 761 1341 5283
Fragments arranged by size:
5283 1341 761
BclI T'GATC_A
Cuts at: 348 1347 3525 4905 348
Size: 999 2178 1380 2828
Fragments arranged by size:
2828 2178 1380 999
BglI GCCn_nnn'nGGC
Cuts at: 793 793
Size: 7385
BglII A'GATC_T
Cuts at: 375 2960 4900 6937 375
Size: 2585 1940 2037 823
Fragments arranged by size:
2585 2037 1940 823
BlpI GC'TnA_GC
Cuts at: 3854 4497 3854
Size: 643 6742
Bme1580I G_kGCm'C
Cuts at: 501 3236 3272 3416 4226 5010 5103 6159 501
Size: 2735 36 144 810 784 93 1056 1727
Fragments arranged by size:
2735 1727 1056 810 784 144 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 1483 2306 2370 2967 3608 3707 3722 4597 4678
Size: 823 64 597 641 99 15 875 81
Cuts at: 4678 5013 7157 1483
Size: 335 2144 1711
Fragments arranged by size:
2144 1711 875 823 641 597 335 99
81 64 15
BmtI G_CTAG'C
Cuts at: 4181 4181
Size: 7385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1422 1986 5752 1422
Size: 564 3766 3055
Fragments arranged by size:
3766 3055 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 2871 3175 3530 3652 3826 1504
Size: 1367 304 355 122 174 5063
Fragments arranged by size:
5063 1367 355 304 174 122
Bpu10I CC'TnA_GC
Cuts at: 1313 3764 3909 4206 1313
Size: 2451 145 297 4492
Fragments arranged by size:
4492 2451 297 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2418 2469 2718 3226 3721 4561 5440 5932
Size: 310 51 249 508 495 840 879 492
Cuts at: 5932 6230 2108
Size: 298 3263
Fragments arranged by size:
3263 879 840 508 495 492 310 298
249 51
BsaI GGTCTCn'nnnn_
Cuts at: 1378 7089 1378
Size: 5711 1674
BsaAI yAC'GTr
Cuts at: 4738 4738
Size: 7385
BsaBI GATnn'nnATC
Cuts at: 6 4899 6936 6
Size: 4893 2037 455
Fragments arranged by size:
4893 2037 455
BsaHI Gr'CG_yC
Cuts at: 165 2448 2937 4816 5064 5766 7262 165
Size: 2283 489 1879 248 702 1496 288
Fragments arranged by size:
2283 1879 1496 702 489 288 248
BsaWI w'CCGG_w
Cuts at: 4574 5095 5773 6047 6194 6605 4574
Size: 521 678 274 147 411 5354
Fragments arranged by size:
5354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1325 1355 2076 2106 2781 2811 1325
Size: 30 721 30 675 30 5899
Fragments arranged by size:
5899 721 675 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1656 2935 3050 3217 3832 1656
Size: 1279 115 167 615 5209
Fragments arranged by size:
5209 1279 615 167 115
BseYI C'CCAG_C
Cuts at: 1030 1068 1434 1861 2043 2135 2151 3229 3327
Size: 38 366 427 182 92 16 1078 98
Cuts at: 3327 3552 3924 4100 4366 4810 5568 6145 6641
Size: 225 372 176 266 444 758 577 496
Cuts at: 6641 1030
Size: 1774
Fragments arranged by size:
1774 1078 758 577 496 444 427 372
366 266 225 182 176 98 92 38
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2341 3229 4289 2341
Size: 888 1060 5437
Fragments arranged by size:
5437 1060 888
BsiEI CG_ry'CG
Cuts at: 104 4927 4973 6181 104
Size: 4823 46 1208 1308
Fragments arranged by size:
4823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 501 2124 4399 5177 5367 6159 501
Size: 1623 2275 778 190 792 1727
Fragments arranged by size:
2275 1727 1623 792 778 190
BsmI GAATG_Cn'
Cuts at: 1167 2418 2928 4049 1167
Size: 1251 510 1121 4503
Fragments arranged by size:
4503 1251 1121 510
Bsp1286I G_dGCh'C
Cuts at: 501 1292 2124 2869 2917 3236 3272 3416 4226
Size: 791 832 745 48 319 36 144 810
Cuts at: 4226 4399 5010 5103 5177 5367 5429 6159 501
Size: 173 611 93 74 190 62 730 1727
Fragments arranged by size:
1727 832 810 791 745 730 611 319
190 173 144 93 74 62 48 36
BspEI T'CCGG_A
Cuts at: 6605 6605
Size: 7385
BspHI T'CATG_A
Cuts at: 6561 6561
Size: 7385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1779 4361 4435 4649 4951 5332 1779
Size: 2582 74 214 302 381 3832
Fragments arranged by size:
3832 2582 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 2432 2969 5677 5731 7215 7380 50
Size: 2382 537 2708 54 1484 165 55
Fragments arranged by size:
2708 2382 1484 537 165 55 54
BsrDI GCAATG_nn'
Cuts at: 754 5297 754
Size: 4543 2842
BsrFI r'CCGG_y
Cuts at: 1892 2288 2351 2444 5383 5564 5773 1892
Size: 396 63 93 2939 181 209 3504
Fragments arranged by size:
3504 2939 396 209 181 93 63
BssSI C'ACGA_G
Cuts at: 2908 5656 6014 2908
Size: 2748 358 4279
Fragments arranged by size:
4279 2748 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4036 4297 4762 4036
Size: 261 465 6659
Fragments arranged by size:
6659 465 261
BstBI TT'CG_AA
Cuts at: 5746 5746
Size: 7385
BstXI CCAn_nnnn'nTGG
Cuts at: 114 258 687 700 2215 114
Size: 144 429 13 1515 5284
Fragments arranged by size:
5284 1515 429 144 13
BstYI r'GATC_y
Cuts at: 1 375 415 622 1692 2143 2737 2960 3161
Size: 374 40 207 1070 451 594 223 201
Cuts at: 3161 3735 4410 4877 4900 5235 5481 6493 6579
Size: 574 675 467 23 335 246 1012 86
Cuts at: 6579 6591 6937 1
Size: 12 346 449
Fragments arranged by size:
1070 1012 675 594 574 467 451 449
374 346 335 246 223 207 201 86
40 23 12
Bsu36I CC'TnA_GG
Cuts at: 965 1329 1749 2918 3815 965
Size: 364 420 1169 897 4535
Fragments arranged by size:
4535 1169 897 420 364
BtgI C'CryG_G
Cuts at: 107 1824 2214 4279 4371 107
Size: 1717 390 2065 92 3121
Fragments arranged by size:
3121 2065 1717 390 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5335 5516 5679 5335
Size: 181 163 7041
Fragments arranged by size:
7041 181 163
BtsI GCAGTG_nn'
Cuts at: 602 656 1074 1511 2175 2418 3137 4301 4628
Size: 54 418 437 664 243 719 1164 327
Cuts at: 4628 602
Size: 3359
Fragments arranged by size:
3359 1164 719 664 437 418 327 243
54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 515 550 3158 3193 4254 4289 515
Size: 35 2608 35 1061 35 3611
Fragments arranged by size:
3611 2608 1061 35 35 35
DraI TTT'AAA
Cuts at: 1602 4384 1602
Size: 2782 4603
DraIII CAC_nnn'GTG
Cuts at: 3534 3534
Size: 7385
DrdI GACnn_nn'nnGTC
Cuts at: 5091 5949 5091
Size: 858 6527
EaeI y'GGCC_r
Cuts at: 1427 1525 2442 3881 4155 4970 5144 5535 5562
Size: 98 917 1439 274 815 174 391 27
Cuts at: 5562 6907 1427
Size: 1345 1905
Fragments arranged by size:
1905 1439 1345 917 815 391 274 174
98 27
EagI C'GGCC_G
Cuts at: 4970 4970
Size: 7385
EarI CTCTTCn'nnn_
Cuts at: 194 414 877 1413 1955 2153 2259 2390 2508
Size: 220 463 536 542 198 106 131 118
Cuts at: 2508 2887 3087 3353 3377 3401 3491 5408 5618
Size: 379 200 266 24 24 90 1917 210
Cuts at: 5618 194
Size: 1961
Fragments arranged by size:
1961 1917 542 536 463 379 266 220
210 200 198 131 118 106 90 24
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1742 2130 5593 5903 6049 7195 1742
Size: 388 3463 310 146 1146 1932
Fragments arranged by size:
3463 1932 1146 388 310 146
EcoICRI GAG'CTC
Cuts at: 2122 4397 2122
Size: 2275 5110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 400 878 1504 1760 2177 2489 2871 3140
Size: 233 478 626 256 417 312 382 269
Cuts at: 3140 3175 3440 3530 3652 3826 5210 5642 6389
Size: 35 265 90 122 174 1384 432 747
Cuts at: 6389 6657 167
Size: 268 895
Fragments arranged by size:
1384 895 747 626 478 432 417 382
312 269 268 265 256 233 174 122
90 35
EcoNI CCTnn'n_nnAGG
Cuts at: 1775 1775
Size: 7385
EcoO109I rG'GnC_Cy
Cuts at: 649 749 851 977 1459 2062 3010 3246 3412
Size: 100 102 126 482 603 948 236 166
Cuts at: 3412 3413 3579 3661 4223 4350 4524 649
Size: 1 166 82 562 127 174 3510
Fragments arranged by size:
3510 948 603 562 482 236 174 166
166 127 126 102 100 82 1
EcoRI G'AATT_C
Cuts at: 628 4389 628
Size: 3761 3624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1344 2257 5404 1344
Size: 913 3147 3325
Fragments arranged by size:
3325 3147 913
FspI TGC'GCA
Cuts at: 5166 5166
Size: 7385
HaeII r_GCGC'y
Cuts at: 168 2451 2595 2733 4819 5067 6089 7068 168
Size: 2283 144 138 2086 248 1022 979 485
Fragments arranged by size:
2283 2086 1022 979 485 248 144 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 918 950 961 993 2076 2108 2329 2361 3115
Size: 32 11 32 1083 32 221 32 754
Cuts at: 3115 3147 3490 3522 5227 5257 5259 5289 918
Size: 32 343 32 1705 30 2 30 3014
Fragments arranged by size:
3014 1705 1083 754 343 221 32 32
32 32 32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 231 3752 4424 231
Size: 3521 672 3192
Fragments arranged by size:
3521 3192 672
KasI G'GCGC_C
Cuts at: 164 2447 4815 5063 164
Size: 2283 2368 248 2486
Fragments arranged by size:
2486 2368 2283 248
KpnI G_GTAC'C
Cuts at: 4405 4405
Size: 7385
MfeI C'AATT_G
Cuts at: 245 245
Size: 7385
MluI A'CGCG_T
Cuts at: 6818 6818
Size: 7385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1427 6056 6240 1427
Size: 4629 184 2572
Fragments arranged by size:
4629 2572 184
MscI TGG'CCA
Cuts at: 1429 1527 3883 4157 5146 1429
Size: 98 2356 274 989 3668
Fragments arranged by size:
3668 2356 989 274 98
MslI CAynn'nnrTG
Cuts at: 112 127 2234 5374 5501 112
Size: 15 2107 3140 127 1996
Fragments arranged by size:
3140 2107 1996 127 15
MspA1I CmG'CkG
Cuts at: 2139 2155 2816 3913 4033 4297 4496 4810 5170
Size: 16 661 1097 120 264 199 314 360
Cuts at: 5170 6183 6428 2139
Size: 1013 245 3096
Fragments arranged by size:
3096 1097 1013 661 360 314 264 245
199 120 16
NaeI GCC'GGC
Cuts at: 2446 5566 2446
Size: 3120 4265
NarI GG'CG_CC
Cuts at: 165 2448 4816 5064 165
Size: 2283 2368 248 2486
Fragments arranged by size:
2486 2368 2283 248
NcoI C'CATG_G
Cuts at: 107 1824 2214 4371 107
Size: 1717 390 2157 3121
Fragments arranged by size:
3121 2157 1717 390
NgoMIV G'CCGG_C
Cuts at: 2444 5564 2444
Size: 3120 4265
NheI G'CTAG_C
Cuts at: 4177 4177
Size: 7385
NspI r_CATG'y
Cuts at: 4006 4252 4438 4938 5845 4006
Size: 246 186 500 907 5546
Fragments arranged by size:
5546 907 500 246 186
PasI CC'CwG_GG
Cuts at: 2080 3418 3574 2080
Size: 1338 156 5891
Fragments arranged by size:
5891 1338 156
PciI A'CATG_T
Cuts at: 5841 5841
Size: 7385
PflMI CCAn_nnn'nTGG
Cuts at: 468 3289 468
Size: 2821 4564
PfoI T'CCnGG_A
Cuts at: 2887 2887
Size: 7385
PmeI GTTT'AAAC
Cuts at: 4384 4384
Size: 7385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6524 6556 6524
Size: 32 7353
PpuMI rG'GwC_Cy
Cuts at: 749 851 977 1459 2062 3010 3246 3579 4350
Size: 102 126 482 603 948 236 333 771
Cuts at: 4350 749
Size: 3784
Fragments arranged by size:
3784 948 771 603 482 333 236 126
102
PshAI GACnn'nnGTC
Cuts at: 3113 3113
Size: 7385
PspOMI G'GGCC_C
Cuts at: 3232 3268 3412 4222 3232
Size: 36 144 810 6395
Fragments arranged by size:
6395 810 144 36
PstI C_TGCA'G
Cuts at: 663 905 3115 4293 4432 5117 663
Size: 242 2210 1178 139 685 2931
Fragments arranged by size:
2931 2210 1178 685 242 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7385
PvuII CAG'CTG
Cuts at: 2155 2816 3913 4033 4297 4810 5170 2155
Size: 661 1097 120 264 513 360 4370
Fragments arranged by size:
4370 1097 661 513 360 264 120
RsrII CG'GwC_CG
Cuts at: 5580 5580
Size: 7385
SacI G_AGCT'C
Cuts at: 2124 4399 2124
Size: 2275 5110
SalI G'TCGA_C
Cuts at: 4422 4422
Size: 7385
SanDI GG'GwC_CC
Cuts at: 749 977 3579 749
Size: 228 2602 4555
Fragments arranged by size:
4555 2602 228
SapI GCTCTTCn'nnn_
Cuts at: 194 2259 3377 5408 5618 194
Size: 2065 1118 2031 210 1961
Fragments arranged by size:
2065 2031 1961 1118 210
SbfI CC_TGCA'GG
Cuts at: 4432 4432
Size: 7385
ScaI AGT'ACT
Cuts at: 6902 6902
Size: 7385
SfcI C'TryA_G
Cuts at: 33 539 659 901 1037 1788 2520 2553 2562
Size: 506 120 242 136 751 732 33 9
Cuts at: 2562 2676 2835 3111 3686 3968 4289 4428 5113
Size: 114 159 276 575 282 321 139 685
Cuts at: 5113 6106 6297 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 751 732 685 575 506 321
282 276 242 191 159 139 136 120
114 33 9
SfoI GGC'GCC
Cuts at: 166 2449 4817 5065 166
Size: 2283 2368 248 2486
Fragments arranged by size:
2486 2368 2283 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
SmaI CCC'GGG
Cuts at: 2743 4407 2743
Size: 1664 5721
SmlI C'TyrA_G
Cuts at: 2123 2433 2484 2733 3241 3700 4540 5455 5947
Size: 310 51 249 508 459 840 915 492
Cuts at: 5947 6209 2123
Size: 262 3299
Fragments arranged by size:
3299 915 840 508 492 459 310 262
249 51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7385
SphI G_CATG'C
Cuts at: 4006 4438 4938 4006
Size: 432 500 6453
Fragments arranged by size:
6453 500 432
SspI AAT'ATT
Cuts at: 2656 2656
Size: 7385
StuI AGG'CCT
Cuts at: 1327 1688 3898 1327
Size: 361 2210 4814
Fragments arranged by size:
4814 2210 361
StyI C'CwwG_G
Cuts at: 107 257 714 894 1227 1550 1683 1824 2214
Size: 150 457 180 333 323 133 141 390
Cuts at: 2214 4371 4519 4873 107
Size: 2157 148 354 2619
Fragments arranged by size:
2619 2157 457 390 354 333 323 180
150 148 141 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1169 1300 4334 4968 5101 5560 5769 1169
Size: 131 3034 634 133 459 209 2785
Fragments arranged by size:
3034 2785 634 459 209 133 131
TatI w'GTAC_w
Cuts at: 215 663 2378 3830 6900 215
Size: 448 1715 1452 3070 700
Fragments arranged by size:
3070 1715 1452 700 448
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1906 1938 3177 3209 6902 6934 1906
Size: 32 1239 32 3693 32 2357
Fragments arranged by size:
3693 2357 1239 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5182 5182
Size: 7385
XbaI T'CTAG_A
Cuts at: 4416 4416
Size: 7385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1128 2215 2314 3150 4880 1128
Size: 1087 99 836 1730 3633
Fragments arranged by size:
3633 1730 1087 836 99
XmaI C'CCGG_G
Cuts at: 2741 4405 2741
Size: 1664 5721
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI NdeI
NotI NruI NsiI PacI PmlI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI