Restriction map for
pF1KA0533
(Circular) (Six-base) MAPSORT of: pF1KA0533.seq Check: 2095 from: 1 to: 14183
pF1KA0533 14183 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1084 1099 1111 1432 1548 1778 2014 2689 2735
Size: 15 12 321 116 230 236 675 46
Cuts at: 2735 2983 3382 5079 5929 6547 1084
Size: 248 399 1697 850 618 8720
Fragments arranged by size:
8720 1697 850 675 618 399 321 248
236 230 116 46 15 12
AatII G_ACGT'C
Cuts at: 822 3398 5666 7634 822
Size: 2576 2268 1968 7371
Fragments arranged by size:
7371 2576 2268 1968
AccI GT'mk_AC
Cuts at: 4256 8908 9548 11221 4256
Size: 4652 640 1673 7218
Fragments arranged by size:
7218 4652 1673 640
Acc65I G'GTAC_C
Cuts at: 8434 8747 11199 8434
Size: 313 2452 11418
Fragments arranged by size:
11418 2452 313
AclI AA'CG_TT
Cuts at: 14023 14023
Size: 14183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 2171 2793 6033 7413 8039 9073 9692 9864
Size: 1952 622 3240 1380 626 1034 619 172
Cuts at: 9864 12008 12440 13187 13455 219
Size: 2144 432 747 268 947
Fragments arranged by size:
3240 2144 1952 1380 1034 947 747 626
622 619 432 268 172
AfeI AGC'GCT
Cuts at: 484 1327 5014 5788 6529 9001 10363 484
Size: 843 3687 774 741 2472 1362 4304
Fragments arranged by size:
4304 3687 2472 1362 843 774 741
AflIII A'CryG_T
Cuts at: 1484 1816 2233 2513 4453 7751 8630 11535 12639
Size: 332 417 280 1940 3298 879 2905 1104
Cuts at: 12639 13616 1484
Size: 977 2051
Fragments arranged by size:
3298 2905 2051 1940 1104 977 879 417
332 280
AgeI A'CCGG_T
Cuts at: 1958 11105 12571 1958
Size: 9147 1466 3570
Fragments arranged by size:
9147 3570 1466
AhdI GACnn_n'nnGTC
Cuts at: 5220 5220
Size: 14183
AleI CACnn'nnGTG
Cuts at: 2236 3673 3916 3982 4135 8041 8878 10870 2236
Size: 1437 243 66 153 3906 837 1992 5549
Fragments arranged by size:
5549 3906 1992 1437 837 243 153 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 686 765 2022 5510 6166 7567 9118 9238 686
Size: 79 1257 3488 656 1401 1551 120 5631
Fragments arranged by size:
5631 3488 1551 1401 1257 656 120 79
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2860 2892 2860
Size: 32 14151
AlwNI CAG_nnn'CTG
Cuts at: 717 1254 1563 2083 3522 3815 4106 4158 4316
Size: 537 309 520 1439 293 291 52 158
Cuts at: 4316 4556 6002 6056 6455 6804 7120 7184 7203
Size: 240 1446 54 399 349 316 64 19
Cuts at: 7203 7362 7590 7734 7779 7803 7854 8121 8187
Size: 159 228 144 45 24 51 267 66
Cuts at: 8187 8419 9015 9072 9663 9887 9954 10574 10830
Size: 232 596 57 591 224 67 620 256
Cuts at: 10830 11165 11488 13055 717
Size: 335 323 1567 1845
Fragments arranged by size:
1845 1567 1446 1439 620 596 591 537
520 399 349 335 323 316 309 293
291 267 256 240 232 228 224 159
158 144 67 66 64 57 54 52
51 45 24 19
ApaI G_GGCC'C
Cuts at: 163 370 2677 3099 3510 4706 4921 5577 5778
Size: 207 2307 422 411 1196 215 656 201
Cuts at: 5778 6110 6717 6781 7092 7107 8413 8551 9830
Size: 332 607 64 311 15 1306 138 1279
Cuts at: 9830 9925 10440 10617 10657 10984 163
Size: 95 515 177 40 327 3362
Fragments arranged by size:
3362 2307 1306 1279 1196 656 607 515
422 411 332 327 311 215 207 201
177 138 95 64 40 15
ApaLI G'TGCA_C
Cuts at: 1071 2612 3490 8221 8518 9983 12953 1071
Size: 1541 878 4731 297 1465 2970 2301
Fragments arranged by size:
4731 2970 2301 1541 1465 878 297
ApoI r'AATT_y
Cuts at: 834 11187 13536 13792 834
Size: 10353 2349 256 1225
Fragments arranged by size:
10353 2349 1225 256
AscI GG'CGCG_CC
Cuts at: 9474 9474
Size: 14183
AseI AT'TA_AT
Cuts at: 20 20
Size: 14183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 14183
AvaI C'yCGr_G
Cuts at: 155 1894 2015 2416 2794 3572 3713 4204 5164
Size: 1739 121 401 378 778 141 491 960
Cuts at: 5164 5176 6510 6835 6878 7091 7157 7367 8285
Size: 12 1334 325 43 213 66 210 918
Cuts at: 8285 9330 9802 9831 10008 10118 10232 10330 10439
Size: 1045 472 29 177 110 114 98 109
Cuts at: 10439 11026 11203 155
Size: 587 177 3135
Fragments arranged by size:
3135 1739 1334 1045 960 918 778 587
491 472 401 378 325 213 210 177
177 141 121 114 110 109 98 66
43 29 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 8739 8772 8739
Size: 33 14150
BamHI G'GATC_C
Cuts at: 2307 10415 11208 2307
Size: 8108 793 5282
Fragments arranged by size:
8108 5282 793
BanI G'GyrC_C
Cuts at: 271 826 1099 1235 1261 1420 1675 1790 1961
Size: 555 273 136 26 159 255 115 171
Cuts at: 1961 2245 2485 2654 2835 3088 3675 3739 4567
Size: 284 240 169 181 253 587 64 828
Cuts at: 4567 4781 5094 5941 6073 6558 6652 6907 7882
Size: 214 313 847 132 485 94 255 975
Cuts at: 7882 8310 8434 8747 8950 9528 9703 9865 10907
Size: 428 124 313 203 578 175 162 1042
Cuts at: 10907 11005 11199 11613 11861 11896 271
Size: 98 194 414 248 35 2558
Fragments arranged by size:
2558 1042 975 847 828 587 578 555
485 428 414 313 313 284 273 255
255 253 248 240 214 203 194 181
175 171 169 162 159 136 132 124
115 98 94 64 35 26
BanII G_rGCy'C
Cuts at: 163 370 1691 2025 2352 2407 2677 3071 3099
Size: 207 1321 334 327 55 270 394 28
Cuts at: 3099 3510 3713 3938 3956 4184 4286 4413 4463
Size: 411 203 225 18 228 102 127 50
Cuts at: 4463 4589 4706 4721 4921 5026 5456 5577 5778
Size: 126 117 15 200 105 430 121 201
Cuts at: 5778 6110 6343 6434 6518 6717 6772 6781 6983
Size: 332 233 91 84 199 55 9 202
Cuts at: 6983 7092 7107 7310 7500 7794 8273 8413 8551
Size: 109 15 203 190 294 479 140 138
Cuts at: 8551 9809 9830 9925 10440 10617 10657 10984 11045
Size: 1258 21 95 515 177 40 327 61
Cuts at: 11045 11197 12227 163
Size: 152 1030 2119
Fragments arranged by size:
2119 1321 1258 1030 515 479 430 411
394 334 332 327 327 294 270 233
228 225 207 203 203 202 201 200
199 190 177 152 140 138 127 126
121 117 109 105 102 95 91 84
61 55 55 50 40 28 21 18
15 15 9
BbeI G_GCGC'C
Cuts at: 275 830 1239 3743 4785 6077 6656 11617 11865
Size: 555 409 2504 1042 1292 579 4961 248
Cuts at: 11865 275
Size: 2593
Fragments arranged by size:
4961 2593 2504 1292 1042 579 555 409
248
BbsI GAAGACnn'nnnn_
Cuts at: 552 2892 3591 3963 6940 7419 8176 8724 9894
Size: 2340 699 372 2977 479 757 548 1170
Cuts at: 9894 552
Size: 4841
Fragments arranged by size:
4841 2977 2340 1170 757 699 548 479
372
BbvCI CC'TCA_GC
Cuts at: 7121 7121
Size: 14183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 450 484 1433 1467 4420 4454 5941 5975 6106
Size: 34 949 34 2953 34 1487 34 131
Cuts at: 6106 6140 6816 6850 6879 6913 7702 7736 7749
Size: 34 676 34 29 34 789 34 13
Cuts at: 7749 7783 7974 8008 8359 8393 8940 8955 8974
Size: 34 191 34 351 34 547 15 19
Cuts at: 8974 8989 9885 9919 450
Size: 15 896 34 4714
Fragments arranged by size:
4714 2953 1487 949 896 789 676 547
351 191 131 34 34 34 34 34
34 34 34 34 34 34 34 29
19 15 15 13
BciVI GTATCCnnnnn_n'
Cuts at: 3570 4522 12081 12842 14183 3570
Size: 952 7559 761 1341 3570
Fragments arranged by size:
7559 3570 1341 952 761
BclI T'GATC_A
Cuts at: 3368 11703 3368
Size: 8335 5848
BglI GCCn_nnn'nGGC
Cuts at: 158 1259 1337 1799 1970 2069 2296 2483 3643
Size: 1101 78 462 171 99 227 187 1160
Cuts at: 3643 5564 5732 5798 6470 6776 6955 7111 7573
Size: 1921 168 66 672 306 179 156 462
Cuts at: 7573 7666 7705 8111 8236 8290 8692 9181 9825
Size: 93 39 406 125 54 402 489 644
Cuts at: 9825 9834 10048 10077 10467 158
Size: 9 214 29 390 3874
Fragments arranged by size:
3874 1921 1160 1101 672 644 489 462
462 406 402 390 306 227 214 187
179 171 168 156 125 99 93 78
66 54 39 29 9
BglII A'GATC_T
Cuts at: 5510 6119 11698 13735 5510
Size: 609 5579 2037 5958
Fragments arranged by size:
5958 5579 2037 609
BlpI GC'TnA_GC
Cuts at: 3346 5074 6517 7174 8197 11295 3346
Size: 1728 1443 657 1023 3098 6234
Fragments arranged by size:
6234 3098 1728 1443 1023 657
Bme1580I G_kGCm'C
Cuts at: 163 370 779 1075 1102 1833 2243 2455 2488
Size: 207 409 296 27 731 410 212 33
Cuts at: 2488 2616 2677 3050 3099 3494 3510 3568 3680
Size: 128 61 373 49 395 16 58 112
Cuts at: 3680 3989 4205 4572 4706 4858 4921 5099 5165
Size: 309 216 367 134 152 63 178 66
Cuts at: 5165 5499 5577 5608 5646 5778 6110 6129 6224
Size: 334 78 31 38 132 332 19 95
Cuts at: 6224 6417 6466 6717 6781 7092 7107 8225 8315
Size: 193 49 251 64 311 15 1118 90
Cuts at: 8315 8413 8522 8551 8898 9588 9708 9830 9925
Size: 98 109 29 347 690 120 122 95
Cuts at: 9925 9987 10440 10617 10657 10984 11010 11808 11901
Size: 62 453 177 40 327 26 798 93
Cuts at: 11901 12957 163
Size: 1056 1389
Fragments arranged by size:
1389 1118 1056 798 731 690 453 410
409 395 373 367 347 334 332 327
311 309 296 251 216 212 207 193
178 177 152 134 132 128 122 120
112 109 98 95 95 93 90 78
66 64 63 62 61 58 49 49
40 38 33 31 29 27 26 19
16 15
BmgBI CAC'GTC
Cuts at: 1380 1485 4167 10215 10868 1380
Size: 105 2682 6048 653 4695
Fragments arranged by size:
6048 4695 2682 653 105
BmrI ACTGGGnnnn_n'
Cuts at: 2296 2467 2626 4542 4787 5907 6113 6257 6393
Size: 171 159 1916 245 1120 206 144 136
Cuts at: 6393 6435 6931 8489 8532 8747 10193 11395 11476
Size: 42 496 1558 43 215 1446 1202 81
Cuts at: 11476 11811 13955 2296
Size: 335 2144 2524
Fragments arranged by size:
2524 2144 1916 1558 1446 1202 1120 496
335 245 215 206 171 159 144 136
81 43 42
BmtI G_CTAG'C
Cuts at: 10918 10918
Size: 14183
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10258 12550 10258
Size: 2292 11891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 643 715 739 802 1766 2755 2761 2835
Size: 105 72 24 63 964 989 6 74
Cuts at: 2835 3310 3857 5368 5497 5566 6826 6835 7354
Size: 475 547 1511 129 69 1260 9 519
Cuts at: 7354 7489 7822 7861 7948 8185 8227 8602 9298
Size: 135 333 39 87 237 42 375 696
Cuts at: 9298 9754 9793 10180 10467 10681 10768 10969 538
Size: 456 39 387 287 214 87 201 3752
Fragments arranged by size:
3752 1511 1260 989 964 696 547 519
475 456 387 375 333 287 237 214
201 135 129 105 87 87 74 72
69 63 42 39 39 24 9 6
Bpu10I CC'TnA_GC
Cuts at: 4698 6437 6973 7121 7457 8334 8420 8560 10294
Size: 1739 536 148 336 877 86 140 1734
Cuts at: 10294 11039 4698
Size: 745 7842
Fragments arranged by size:
7842 1739 1734 877 745 536 336 148
140 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 8377 9400 9668 11359 12238 12730 13028 8377
Size: 1023 268 1691 879 492 298 9532
Fragments arranged by size:
9532 1691 1023 879 492 298 268
BsaI GGTCTCn'nnnn_
Cuts at: 2949 5257 5677 6875 10395 13887 2949
Size: 2308 420 1198 3520 3492 3245
Fragments arranged by size:
3520 3492 3245 2308 1198 420
BsaAI yAC'GTr
Cuts at: 567 859 2355 2514 3135 4454 5148 7754 11536
Size: 292 1496 159 621 1319 694 2606 3782
Cuts at: 11536 567
Size: 3214
Fragments arranged by size:
3782 3214 2606 1496 1319 694 621 292
159
BsaBI GATnn'nnATC
Cuts at: 6 5335 11697 13734 6
Size: 5329 6362 2037 455
Fragments arranged by size:
6362 5329 2037 455
BsaHI Gr'CG_yC
Cuts at: 272 819 827 1236 1313 1528 2645 3395 3740
Size: 547 8 409 77 215 1117 750 345
Cuts at: 3740 4782 5663 5741 6074 6653 7286 7631 9290
Size: 1042 881 78 333 579 633 345 1659
Cuts at: 9290 9604 10804 11614 11862 12564 14060 272
Size: 314 1200 810 248 702 1496 395
Fragments arranged by size:
1659 1496 1200 1117 1042 881 810 750
702 633 579 547 409 395 345 345
333 314 248 215 78 77 8
BsaWI w'CCGG_w
Cuts at: 1958 4525 6764 7046 9355 10412 10551 10771 11105
Size: 2567 2239 282 2309 1057 139 220 334
Cuts at: 11105 11372 11893 12571 12845 12992 13403 1958
Size: 267 521 678 274 147 411 2738
Fragments arranged by size:
2738 2567 2309 2239 1057 678 521 411
334 282 274 267 220 147 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 472 502 782 812 2860 2890 2950 2980 3283
Size: 30 280 30 2048 30 60 30 303
Cuts at: 3283 3313 4785 4815 8754 8784 9350 9380 9535
Size: 30 1472 30 3939 30 566 30 155
Cuts at: 9535 9565 10375 10405 472
Size: 30 810 30 4250
Fragments arranged by size:
4250 3939 2048 1472 810 566 303 280
155 60 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 319 666 2758 2971 3232 3700 4294 4699
Size: 87 347 2092 213 261 468 594 405
Cuts at: 4699 5464 5505 6649 7204 7477 8842 9829 10231
Size: 765 41 1144 555 273 1365 987 402
Cuts at: 10231 10234 11103 232
Size: 3 869 3312
Fragments arranged by size:
3312 2092 1365 1144 987 869 765 594
555 468 405 402 347 273 261 213
87 41 3
BseYI C'CCAG_C
Cuts at: 195 915 2122 2407 3019 3218 3512 3794 3885
Size: 720 1207 285 612 199 294 282 91
Cuts at: 3885 3993 4345 5267 5559 5824 5991 7232 7590
Size: 108 352 922 292 265 167 1241 358
Cuts at: 7590 7793 7854 7892 8051 8180 8535 9201 10400
Size: 203 61 38 159 129 355 666 1199
Cuts at: 10400 10493 11010 11169 11608 12366 12943 13439 195
Size: 93 517 159 439 758 577 496 939
Fragments arranged by size:
1241 1207 1199 939 922 758 720 666
612 577 517 496 439 358 355 352
294 292 285 282 265 203 199 167
159 159 129 108 93 91 61 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 408 735 1806 2146 2214 2704 2970 3183
Size: 182 327 1071 340 68 490 266 213
Cuts at: 3183 3360 3517 3643 4000 4782 4933 4956 5153
Size: 177 157 126 357 782 151 23 197
Cuts at: 5153 5239 6150 6706 7183 7423 7723 7802 8065
Size: 86 911 556 477 240 300 79 263
Cuts at: 8065 8362 9435 9928 10579 11074 226
Size: 297 1073 493 651 495 3335
Fragments arranged by size:
3335 1073 1071 911 782 651 556 495
493 490 477 357 340 327 300 297
266 263 240 213 197 182 177 157
151 126 86 79 68 23
BsiEI CG_ry'CG
Cuts at: 104 607 645 1110 1917 3281 4074 5907 6332
Size: 503 38 465 807 1364 793 1833 425
Cuts at: 6332 7224 8912 11725 11771 12979 104
Size: 892 1688 2813 46 1208 1308
Fragments arranged by size:
2813 1833 1688 1364 1308 1208 892 807
793 503 465 425 46 38
BsiHKAI G_wGCw'C
Cuts at: 1075 1691 2616 3125 3494 4184 4589 4721 5026
Size: 616 925 509 369 690 405 132 305
Cuts at: 5026 5080 5456 6829 6983 7310 8225 8273 8522
Size: 54 376 1373 154 327 915 48 249
Cuts at: 8522 9044 9809 9976 9987 10490 10943 11197 11975
Size: 522 765 167 11 503 453 254 778
Cuts at: 11975 12165 12957 1075
Size: 190 792 2301
Fragments arranged by size:
2301 1373 925 915 792 778 765 690
616 522 509 503 453 405 376 369
327 305 254 249 190 167 154 132
54 48 11
BsmI GAATG_Cn'
Cuts at: 2101 2101
Size: 14183
BsmBI CGTCTCn'nnnn_
Cuts at: 6784 6784
Size: 14183
Bsp1286I G_dGCh'C
Cuts at: 163 370 779 1075 1102 1691 1833 2025 2243
Size: 207 409 296 27 589 142 192 218
Cuts at: 2243 2352 2407 2455 2488 2616 2677 3050 3071
Size: 109 55 48 33 128 61 373 21
Cuts at: 3071 3099 3125 3494 3510 3568 3680 3713 3938
Size: 28 26 369 16 58 112 33 225
Cuts at: 3938 3956 3989 4184 4205 4286 4413 4463 4572
Size: 18 33 195 21 81 127 50 109
Cuts at: 4572 4589 4706 4721 4858 4921 5026 5080 5099
Size: 17 117 15 137 63 105 54 19
Cuts at: 5099 5165 5456 5499 5577 5608 5646 5778 6110
Size: 66 291 43 78 31 38 132 332
Cuts at: 6110 6129 6224 6343 6417 6434 6466 6518 6717
Size: 19 95 119 74 17 32 52 199
Cuts at: 6717 6772 6781 6829 6983 7092 7107 7310 7500
Size: 55 9 48 154 109 15 203 190
Cuts at: 7500 7794 8225 8273 8315 8413 8522 8551 8898
Size: 294 431 48 42 98 109 29 347
Cuts at: 8898 9044 9588 9708 9809 9830 9925 9976 9987
Size: 146 544 120 101 21 95 51 11
Cuts at: 9987 10440 10490 10617 10657 10943 10984 11010 11045
Size: 453 50 127 40 286 41 26 35
Cuts at: 11045 11197 11808 11901 11975 12165 12227 12957 163
Size: 152 611 93 74 190 62 730 1389
Fragments arranged by size:
1389 730 611 589 544 453 431 409
373 369 347 332 296 294 291 286
225 218 207 203 199 195 192 190
190 154 152 146 142 137 132 128
127 127 120 119 117 112 109 109
109 109 105 101 98 95 95 93
81 78 74 74 66 63 62 61
58 55 55 54 52 51 50 50
48 48 48 43 42 41 40 38
35 33 33 33 32 31 29 28
27 26 26 21 21 21 19 19
18 17 17 16 15 15 11 9
BspEI T'CCGG_A
Cuts at: 6764 7046 13403 6764
Size: 282 6357 7544
Fragments arranged by size:
7544 6357 282
BspHI T'CATG_A
Cuts at: 5456 5804 13359 5456
Size: 348 7555 6280
Fragments arranged by size:
7555 6280 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 304 1084 1099 1111 1432 1548 1778 2014 2689
Size: 780 15 12 321 116 230 236 675
Cuts at: 2689 2726 2735 2954 2983 3382 4804 5079 5147
Size: 37 9 219 29 399 1422 275 68
Cuts at: 5147 5929 6547 6758 7265 8411 8720 9470 10815
Size: 782 618 211 507 1146 309 750 1345
Cuts at: 10815 11233 11447 11749 12130 304
Size: 418 214 302 381 2357
Fragments arranged by size:
2357 1422 1345 1146 782 780 750 675
618 507 418 399 381 321 309 302
275 236 230 219 214 211 116 68
37 29 15 12 9
BsrBI CCG'CTC
Cuts at: 50 627 699 1349 1476 2936 5106 5955 6362
Size: 577 72 650 127 1460 2170 849 407
Cuts at: 6362 7068 8082 8900 9240 9534 12475 12529 14013
Size: 706 1014 818 340 294 2941 54 1484
Cuts at: 14013 14178 50
Size: 165 55
Fragments arranged by size:
2941 2170 1484 1460 1014 849 818 706
650 577 407 340 294 165 127 72
55 54
BsrDI GCAATG_nn'
Cuts at: 468 5592 8273 9738 10305 12095 468
Size: 5124 2681 1465 567 1790 2556
Fragments arranged by size:
5124 2681 2556 1790 1465 567
BsrFI r'CCGG_y
Cuts at: 376 962 1232 1309 1454 1570 1912 1958 2201
Size: 586 270 77 145 116 342 46 243
Cuts at: 2201 2290 2488 2594 2651 4069 4211 5084 5352
Size: 89 198 106 57 1418 142 873 268
Cuts at: 5352 6327 6649 6904 7223 7243 8953 9445 10466
Size: 975 322 255 319 20 1710 492 1021
Cuts at: 10466 10910 11105 12181 12362 12571 376
Size: 444 195 1076 181 209 1988
Fragments arranged by size:
1988 1710 1418 1076 1021 975 873 586
492 444 342 322 319 270 268 255
243 209 198 195 181 145 142 116
106 89 77 57 46 20
BsrGI T'GTAC_A
Cuts at: 3545 8456 9251 3545
Size: 4911 795 8477
Fragments arranged by size:
8477 4911 795
BssHII G'CGCG_C
Cuts at: 316 1694 9474 316
Size: 1378 7780 5025
Fragments arranged by size:
7780 5025 1378
BssSI C'ACGA_G
Cuts at: 4237 4846 5101 6673 7237 9022 9100 12454 12812
Size: 609 255 1572 564 1785 78 3354 358
Cuts at: 12812 4237
Size: 5608
Fragments arranged by size:
5608 3354 1785 1572 609 564 358 255
78
BstAPI GCAn_nnn'nTGC
Cuts at: 1563 1622 2087 2727 3522 3557 4988 5094 5915
Size: 59 465 640 795 35 1431 106 821
Cuts at: 5915 6002 6173 6455 6558 7521 7701 7956 8064
Size: 87 171 282 103 963 180 255 108
Cuts at: 8064 9015 10830 11005 11560 1563
Size: 951 1815 175 555 4186
Fragments arranged by size:
4186 1815 1431 963 951 821 795 640
555 465 282 255 180 175 171 108
106 103 87 59 35
BstBI TT'CG_AA
Cuts at: 12544 12544
Size: 14183
BstEII G'GTnAC_C
Cuts at: 954 6031 10554 954
Size: 5077 4523 4583
Fragments arranged by size:
5077 4583 4523
BstXI CCAn_nnnn'nTGG
Cuts at: 1867 2038 2856 3226 3918 4498 4657 5589 6103
Size: 171 818 370 692 580 159 932 514
Cuts at: 6103 6466 6705 8010 8479 10158 10626 1867
Size: 363 239 1305 469 1679 468 5424
Fragments arranged by size:
5424 1679 1305 932 818 692 580 514
469 468 370 363 239 171 159
BstYI r'GATC_y
Cuts at: 1 2307 3864 5489 5510 6119 8361 10415 11208
Size: 2306 1557 1625 21 609 2242 2054 793
Cuts at: 11208 11675 11698 12033 12279 13291 13377 13389 13735
Size: 467 23 335 246 1012 86 12 346
Cuts at: 13735 1
Size: 449
Fragments arranged by size:
2306 2242 2054 1625 1557 1012 793 609
467 449 346 335 246 86 23 21
12
Bsu36I CC'TnA_GG
Cuts at: 1223 2321 2446 2759 3808 5153 9664 9821 10108
Size: 1098 125 313 1049 1345 4511 157 287
Cuts at: 10108 1223
Size: 5298
Fragments arranged by size:
5298 4511 1345 1098 1049 313 287 157
125
BtgI C'CryG_G
Cuts at: 107 371 510 632 950 1014 1049 1138 1977
Size: 264 139 122 318 64 35 89 839
Cuts at: 1977 3005 3335 3735 3965 4049 4812 5006 5291
Size: 1028 330 400 230 84 763 194 285
Cuts at: 5291 5382 5706 6081 6389 6704 6869 7964 8214
Size: 91 324 375 308 315 165 1095 250
Cuts at: 8214 8356 8920 9245 9369 9489 9495 9737 10976
Size: 142 564 325 124 120 6 242 1239
Cuts at: 10976 11045 11142 107
Size: 69 97 3148
Fragments arranged by size:
3148 1239 1095 1028 839 763 564 400
375 330 325 324 318 315 308 285
264 250 242 230 194 165 142 139
124 122 120 97 91 89 84 69
64 35 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 1049 1919 6726 12133 12314 12477 264
Size: 785 870 4807 5407 181 163 1970
Fragments arranged by size:
5407 4807 1970 870 785 181 163
BtsI GCAGTG_nn'
Cuts at: 1075 2081 2139 2197 3390 3419 3566 4314 4856
Size: 1006 58 58 1193 29 147 748 542
Cuts at: 4856 4897 6222 6372 7286 7507 7814 8138 8384
Size: 41 1325 150 914 221 307 324 246
Cuts at: 8384 9145 10250 10723 11156 11426 1075
Size: 761 1105 473 433 270 3832
Fragments arranged by size:
3832 1325 1193 1105 1006 914 761 748
542 473 433 324 307 270 246 221
150 147 58 58 41 29
ClaI AT'CG_AT
Cuts at: 470 8567 470
Size: 8097 6086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 9864 9899 9864
Size: 35 14148
DraI TTT'AAA
Cuts at: 11182 11182
Size: 14183
DraIII CAC_nnn'GTG
Cuts at: 142 2461 3557 5915 6173 6618 8154 8526 10977
Size: 2319 1096 2358 258 445 1536 372 2451
Cuts at: 10977 142
Size: 3348
Fragments arranged by size:
3348 2451 2358 2319 1536 1096 445 372
258
DrdI GACnn_nn'nnGTC
Cuts at: 952 1533 8728 11889 12747 952
Size: 581 7195 3161 858 2388
Fragments arranged by size:
7195 3161 2388 858 581
EaeI y'GGCC_r
Cuts at: 263 374 604 642 1017 1194 1572 1751 1914
Size: 111 230 38 375 177 378 179 163
Cuts at: 1914 2490 2520 2858 2969 3278 3485 4071 4500
Size: 576 30 338 111 309 207 586 429
Cuts at: 4500 4749 5370 5468 5552 5579 5904 6329 6861
Size: 249 621 98 84 27 325 425 532
Cuts at: 6861 6902 6947 7010 7055 7205 7418 7725 7805
Size: 41 45 63 45 150 213 307 80
Cuts at: 7805 8189 8256 8625 8716 9080 9173 9284 9492
Size: 384 67 369 91 364 93 111 208
Cuts at: 9492 10302 10374 10526 10899 11048 11768 11942 12333
Size: 810 72 152 373 149 720 174 391
Cuts at: 12333 12360 13705 263
Size: 27 1345 741
Fragments arranged by size:
1345 810 741 720 621 586 576 532
429 425 391 384 378 375 373 369
364 338 325 309 307 249 230 213
208 207 179 177 174 163 152 150
149 111 111 111 98 93 91 84
80 72 67 63 45 45 41 38
30 27 27
EagI C'GGCC_G
Cuts at: 604 642 1914 3278 4071 5904 6329 11768 604
Size: 38 1272 1364 793 1833 425 5439 3019
Fragments arranged by size:
5439 3019 1833 1364 1272 793 425 38
EarI CTCTTCn'nnn_
Cuts at: 4399 5506 5680 6052 7820 9049 9352 10252 10294
Size: 1107 174 372 1768 1229 303 900 42
Cuts at: 10294 10576 12206 12416 4399
Size: 282 1630 210 6166
Fragments arranged by size:
6166 1768 1630 1229 1107 900 372 303
282 210 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 874 7759 8235 10855 12391 12701 12847 13993 874
Size: 6885 476 2620 1536 310 146 1146 1064
Fragments arranged by size:
6885 2620 1536 1146 1064 476 310 146
EcoICRI GAG'CTC
Cuts at: 1689 4182 4587 4719 5024 5454 6981 7308 8271
Size: 2493 405 132 305 430 1527 327 963
Cuts at: 8271 9807 11195 1689
Size: 1536 1388 4677
Fragments arranged by size:
4677 2493 1536 1527 1388 963 430 405
327 305 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 538 643 715 739 802 1766 2171 2755
Size: 319 105 72 24 63 964 405 584
Cuts at: 2755 2761 2793 2835 3310 3857 5368 5497 5566
Size: 6 32 42 475 547 1511 129 69
Cuts at: 5566 6033 6826 6835 7354 7413 7489 7822 7861
Size: 467 793 9 519 59 76 333 39
Cuts at: 7861 7948 8039 8185 8227 8602 9073 9298 9692
Size: 87 91 146 42 375 471 225 394
Cuts at: 9692 9754 9793 9864 10180 10467 10681 10768 10969
Size: 62 39 71 316 287 214 87 201
Cuts at: 10969 12008 12440 13187 13455 219
Size: 1039 432 747 268 947
Fragments arranged by size:
1511 1039 964 947 793 747 584 547
519 475 471 467 432 405 394 375
333 319 316 287 268 225 214 201
146 129 105 91 87 87 76 72
71 69 63 62 59 42 42 39
39 32 24 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 265 553 681 1775 2320 4276 6232 6566 6838
Size: 288 128 1094 545 1956 1956 334 272
Cuts at: 6838 7186 7261 7450 7738 8125 9133 9637 265
Size: 348 75 189 288 387 1008 504 4811
Fragments arranged by size:
4811 1956 1956 1094 1008 545 504 387
348 334 288 288 272 189 128 75
EcoO109I rG'GnC_Cy
Cuts at: 159 160 309 342 366 367 945 1431 1607
Size: 1 149 33 24 1 578 486 176
Cuts at: 1607 1632 2673 2674 3096 3294 3507 4155 4447
Size: 25 1041 1 422 198 213 648 292
Cuts at: 4447 4553 4702 4917 4918 4946 5193 5237 5573
Size: 106 149 215 1 28 247 44 336
Cuts at: 5573 5574 5774 6107 6394 6778 6875 7095 7103
Size: 1 200 333 287 384 97 220 8
Cuts at: 7103 7104 7731 7776 7889 8103 8118 8409 8548
Size: 1 627 45 113 214 15 291 139
Cuts at: 8548 8858 8916 9393 9826 9827 10018 10436 10604
Size: 310 58 477 433 1 191 418 168
Cuts at: 10604 10613 10614 10654 10827 10981 11031 11322 159
Size: 9 1 40 173 154 50 291 3020
Fragments arranged by size:
3020 1041 648 627 578 486 477 433
422 418 384 336 333 310 292 291
291 287 247 220 215 214 213 200
198 191 176 173 168 154 149 149
139 113 106 97 58 50 45 44
40 33 28 25 24 15 9 8
1 1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 11187 11187
Size: 14183
EcoRV GAT'ATC
Cuts at: 987 987
Size: 14183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2565 12202 2565
Size: 9637 4546
FseI GG_CCGG'CC
Cuts at: 1916 7247 1916
Size: 5331 8852
FspI TGC'GCA
Cuts at: 4320 5533 8350 11964 4320
Size: 1213 2817 3614 6539
Fragments arranged by size:
6539 3614 2817 1213
HaeII r_GCGC'y
Cuts at: 193 275 486 830 940 1239 1329 3172 3743
Size: 82 211 344 110 299 90 1843 571
Cuts at: 3743 4785 5016 5790 5831 6077 6258 6456 6531
Size: 1042 231 774 41 246 181 198 75
Cuts at: 6531 6656 7332 7476 8334 9003 9446 9517 9575
Size: 125 676 144 858 669 443 71 58
Cuts at: 9575 10288 10365 11617 11865 12887 13866 193
Size: 713 77 1252 248 1022 979 510
Fragments arranged by size:
1843 1252 1042 1022 979 858 774 713
676 669 571 510 443 344 299 248
246 231 211 198 181 144 125 110
90 82 77 75 71 58 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 675 707 1162 1194 2950 2982 4783 4815 5206
Size: 32 455 32 1756 32 1801 32 391
Cuts at: 5206 5238 6970 7002 8642 8674 9350 9382 12025
Size: 32 1732 32 1640 32 676 32 2643
Cuts at: 12025 12055 12057 12087 675
Size: 30 2 30 2771
Fragments arranged by size:
2771 2643 1801 1756 1732 1640 676 455
391 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 534 1317 2913 7635 8909 11222 534
Size: 783 1596 4722 1274 2313 3495
Fragments arranged by size:
4722 3495 2313 1596 1274 783
KasI G'GCGC_C
Cuts at: 271 826 1235 3739 4781 6073 6652 11613 11861
Size: 555 409 2504 1042 1292 579 4961 248
Cuts at: 11861 271
Size: 2593
Fragments arranged by size:
4961 2593 2504 1292 1042 579 555 409
248
KpnI G_GTAC'C
Cuts at: 8438 8751 11203 8438
Size: 313 2452 11418
Fragments arranged by size:
11418 2452 313
MfeI C'AATT_G
Cuts at: 6895 6895
Size: 14183
MluI A'CGCG_T
Cuts at: 13616 13616
Size: 14183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1430 5894 7697 12854 13038 1430
Size: 4464 1803 5157 184 2575
Fragments arranged by size:
5157 4464 2575 1803 184
MscI TGG'CCA
Cuts at: 2860 5372 5470 5554 5581 7012 7207 7420 7727
Size: 2512 98 84 27 1431 195 213 307
Cuts at: 7727 7807 8191 8627 9494 10304 10901 11944 2860
Size: 80 384 436 867 810 597 1043 5099
Fragments arranged by size:
5099 2512 1431 1043 867 810 597 436
384 307 213 195 98 84 80 27
MslI CAynn'nnrTG
Cuts at: 1025 2236 2929 3043 3673 3916 3982 4135 4490
Size: 1211 693 114 630 243 66 153 355
Cuts at: 4490 5461 8041 8494 8809 8878 10624 10870 11050
Size: 971 2580 453 315 69 1746 246 180
Cuts at: 11050 12172 12299 1025
Size: 1122 127 2909
Fragments arranged by size:
2909 2580 1746 1211 1122 971 693 630
453 355 315 246 243 180 153 127
114 69 66
MspA1I CmG'CkG
Cuts at: 171 504 512 852 1004 1112 1391 1763 1919
Size: 333 8 340 152 108 279 372 156
Cuts at: 1919 2060 2126 2198 2378 2432 2438 2591 2813
Size: 141 66 72 180 54 6 153 222
Cuts at: 2813 3180 3504 3722 3831 3849 3889 3916 4517
Size: 367 324 218 109 18 40 27 601
Cuts at: 4517 4748 4787 4826 4988 5271 5391 5397 5484
Size: 231 39 39 162 283 120 6 87
Cuts at: 5484 5723 5843 6023 6113 6218 6266 6476 6497
Size: 239 120 180 90 105 48 210 21
Cuts at: 6497 6521 6804 7041 7120 7173 7590 7599 7926
Size: 24 283 237 79 53 417 9 327
Cuts at: 7926 8055 8184 8346 8535 8596 8868 9035 9324
Size: 129 129 162 189 61 272 167 289
Cuts at: 9324 9912 10379 10571 10688 11002 11294 11608 11968
Size: 588 467 192 117 314 292 314 360
Cuts at: 11968 12981 13226 171
Size: 1013 245 1128
Fragments arranged by size:
1128 1013 601 588 467 417 372 367
360 340 333 327 324 314 314 292
289 283 283 279 272 245 239 237
231 222 218 210 192 189 180 180
167 162 162 156 153 152 141 129
129 120 120 117 109 108 105 90
87 79 72 66 61 54 53 48
40 39 39 27 24 21 18 9
8 6 6
NaeI GCC'GGC
Cuts at: 378 1456 1572 1914 2203 4071 5354 6651 6906
Size: 1078 116 342 289 1868 1283 1297 255
Cuts at: 6906 7245 9447 12364 378
Size: 339 2202 2917 2197
Fragments arranged by size:
2917 2202 2197 1868 1297 1283 1078 342
339 289 255 116
NarI GG'CG_CC
Cuts at: 272 827 1236 3740 4782 6074 6653 11614 11862
Size: 555 409 2504 1042 1292 579 4961 248
Cuts at: 11862 272
Size: 2593
Fragments arranged by size:
4961 2593 2504 1292 1042 579 555 409
248
NcoI C'CATG_G
Cuts at: 107 632 1977 4812 5291 5706 6389 6704 7964
Size: 525 1345 2835 479 415 683 315 1260
Cuts at: 7964 9489 11045 107
Size: 1525 1556 3245
Fragments arranged by size:
3245 2835 1556 1525 1345 1260 683 525
479 415 315
NgoMIV G'CCGG_C
Cuts at: 376 1454 1570 1912 2201 4069 5352 6649 6904
Size: 1078 116 342 289 1868 1283 1297 255
Cuts at: 6904 7243 9445 12362 376
Size: 339 2202 2917 2197
Fragments arranged by size:
2917 2202 2197 1868 1297 1283 1078 342
339 289 255 116
NheI G'CTAG_C
Cuts at: 10914 10914
Size: 14183
NotI GC'GGCC_GC
Cuts at: 5904 5904
Size: 14183
NspI r_CATG'y
Cuts at: 1820 2003 2237 7402 8002 8634 10095 11236 11736
Size: 183 234 5165 600 632 1461 1141 500
Cuts at: 11736 12643 1820
Size: 907 3360
Fragments arranged by size:
5165 3360 1461 1141 907 632 600 500
234 183
PasI CC'CwG_GG
Cuts at: 1611 2670 4326 6466 6774 8689 9396 10608 1611
Size: 1059 1656 2140 308 1915 707 1212 5186
Fragments arranged by size:
5186 2140 1915 1656 1212 1059 707 308
PciI A'CATG_T
Cuts at: 1816 2233 8630 12639 1816
Size: 417 6397 4009 3360
Fragments arranged by size:
6397 4009 3360 417
PflMI CCAn_nnn'nTGG
Cuts at: 927 1254 3477 4671 6618 6710 6870 6900 7203
Size: 327 2223 1194 1947 92 160 30 303
Cuts at: 7203 8121 8187 10977 927
Size: 918 66 2790 4133
Fragments arranged by size:
4133 2790 2223 1947 1194 918 327 303
160 92 66 30
PfoI T'CCnGG_A
Cuts at: 2711 3125 4274 4712 5345 6629 7625 7838 8204
Size: 414 1149 438 633 1284 996 213 366
Cuts at: 8204 8660 2711
Size: 456 8234
Fragments arranged by size:
8234 1284 1149 996 633 456 438 414
366 213
PmeI GTTT'AAAC
Cuts at: 11182 11182
Size: 14183
PmlI CAC'GTG
Cuts at: 567 2355 2514 4454 5148 7754 567
Size: 1788 159 1940 694 2606 6996
Fragments arranged by size:
6996 2606 1940 1788 694 159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 2892 13322 13354 2860
Size: 32 10430 32 3689
Fragments arranged by size:
10430 3689 32 32
PpuMI rG'GwC_Cy
Cuts at: 342 945 1431 1607 1632 4946 5237 6394 6875
Size: 603 486 176 25 3314 291 1157 481
Cuts at: 6875 7095 7889 8118 8858 8916 10827 342
Size: 220 794 229 740 58 1911 3698
Fragments arranged by size:
3698 3314 1911 1157 794 740 603 486
481 291 229 220 176 58 25
PshAI GACnn'nnGTC
Cuts at: 2644 2644
Size: 14183
PspOMI G'GGCC_C
Cuts at: 159 366 2673 3095 3506 4702 4917 5573 5774
Size: 207 2307 422 411 1196 215 656 201
Cuts at: 5774 6106 6713 6777 7088 7103 8409 8547 9826
Size: 332 607 64 311 15 1306 138 1279
Cuts at: 9826 9921 10436 10613 10653 10980 159
Size: 95 515 177 40 327 3362
Fragments arranged by size:
3362 2307 1306 1279 1196 656 607 515
422 411 332 327 311 215 207 201
177 138 95 64 40 15
PspXI vC'TCGA_Gb
Cuts at: 2794 2794
Size: 14183
PstI C_TGCA'G
Cuts at: 1070 1597 1876 1884 2437 2686 3179 4552 5321
Size: 527 279 8 553 249 493 1373 769
Cuts at: 5321 5396 5489 6022 6451 6544 6755 7140 7388
Size: 75 93 533 429 93 211 385 248
Cuts at: 7388 7517 7697 7739 8060 8408 8556 9152 9638
Size: 129 180 42 321 348 148 596 486
Cuts at: 9638 9947 10034 11001 11230 11915 1070
Size: 309 87 967 229 685 3338
Fragments arranged by size:
3338 1373 967 769 685 596 553 533
527 493 486 429 385 348 321 309
279 249 248 229 211 180 148 129
93 93 87 75 42 8
PvuI CG_AT'CG
Cuts at: 104 104
Size: 14183
PvuII CAG'CTG
Cuts at: 2126 2198 2438 2591 2813 3180 3722 3889 5391
Size: 72 240 153 222 367 542 167 1502
Cuts at: 5391 5397 5484 5843 6804 7041 7120 7173 7590
Size: 6 87 359 961 237 79 53 417
Cuts at: 7590 7599 7926 8055 8184 8346 8535 8596 8868
Size: 9 327 129 129 162 189 61 272
Cuts at: 8868 9324 11002 11608 11968 2126
Size: 456 1678 606 360 4341
Fragments arranged by size:
4341 1678 1502 961 606 542 456 417
367 360 359 327 272 240 237 222
189 167 162 153 129 129 87 79
72 61 53 9 6
RsrII CG'GwC_CG
Cuts at: 12378 12378
Size: 14183
SacI G_AGCT'C
Cuts at: 1691 4184 4589 4721 5026 5456 6983 7310 8273
Size: 2493 405 132 305 430 1527 327 963
Cuts at: 8273 9809 11197 1691
Size: 1536 1388 4677
Fragments arranged by size:
4677 2493 1536 1527 1388 963 430 405
327 305 132
SacII CC_GC'GG
Cuts at: 513 513
Size: 14183
SalI G'TCGA_C
Cuts at: 8907 11220 8907
Size: 2313 11870
SanDI GG'GwC_CC
Cuts at: 945 1607 5237 6394 6875 8916 945
Size: 662 3630 1157 481 2041 6212
Fragments arranged by size:
6212 3630 2041 1157 662 481
SapI GCTCTTCn'nnn_
Cuts at: 6052 9049 9352 10294 12206 12416 6052
Size: 2997 303 942 1912 210 7819
Fragments arranged by size:
7819 2997 1912 942 303 210
SbfI CC_TGCA'GG
Cuts at: 1884 7388 7697 8408 11230 1884
Size: 5504 309 711 2822 4837
Fragments arranged by size:
5504 4837 2822 711 309
ScaI AGT'ACT
Cuts at: 412 763 13700 412
Size: 351 12937 895
Fragments arranged by size:
12937 895 351
SexAI A'CCwGG_T
Cuts at: 5928 5928
Size: 14183
SfcI C'TryA_G
Cuts at: 33 1066 1593 1872 1880 2433 2682 3175 4257
Size: 1033 527 279 8 553 249 493 1082
Cuts at: 4257 4442 4548 5317 5392 5485 6018 6447 6540
Size: 185 106 769 75 93 533 429 93
Cuts at: 6540 6751 7136 7384 7513 7680 7693 7735 8056
Size: 211 385 248 129 167 13 42 321
Cuts at: 8056 8404 8552 9030 9148 9634 9655 9726 9943
Size: 348 148 478 118 486 21 71 217
Cuts at: 9943 10030 10997 11226 11911 12904 13095 33
Size: 87 967 229 685 993 191 1121
Fragments arranged by size:
1121 1082 1033 993 967 769 685 553
533 527 493 486 478 429 385 348
321 279 249 248 229 217 211 191
185 167 148 129 118 106 93 93
87 75 71 42 21 13 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 158 7573 7666 8111 158
Size: 7415 93 445 6230
Fragments arranged by size:
7415 6230 445 93
SfoI GGC'GCC
Cuts at: 273 828 1237 3741 4783 6075 6654 11615 11863
Size: 555 409 2504 1042 1292 579 4961 248
Cuts at: 11863 273
Size: 2593
Fragments arranged by size:
4961 2593 2504 1292 1042 579 555 409
248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 14183
SgrAI Cr'CCGG_yG
Cuts at: 1309 1309
Size: 14183
SmaI CCC'GGG
Cuts at: 157 3574 3715 6512 7093 7159 7369 8287 10010
Size: 3417 141 2797 581 66 210 918 1723
Cuts at: 10010 10441 11205 157
Size: 431 764 3135
Fragments arranged by size:
3417 3135 2797 1723 918 764 581 431
210 141 66
SmlI C'TyrA_G
Cuts at: 2794 8392 9415 9683 11338 12253 12745 13007 2794
Size: 5598 1023 268 1655 915 492 262 3970
Fragments arranged by size:
5598 3970 1655 1023 915 492 268 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 14183
SphI G_CATG'C
Cuts at: 2003 7402 10095 11236 11736 2003
Size: 5399 2693 1141 500 4450
Fragments arranged by size:
5399 4450 2693 1141 500
SrfI GCCC'GGGC
Cuts at: 10441 10441
Size: 14183
StuI AGG'CCT
Cuts at: 2527 3196 6202 7669 8889 9851 10051 10238 10328
Size: 669 3006 1467 1220 962 200 187 90
Cuts at: 10328 2527
Size: 6382
Fragments arranged by size:
6382 3006 1467 1220 962 669 200 187
90
StyI C'CwwG_G
Cuts at: 107 632 869 1636 1671 1977 4812 4941 5291
Size: 525 237 767 35 306 2835 129 350
Cuts at: 5291 5706 6389 6704 7964 8003 8669 9489 11045
Size: 415 683 315 1260 39 666 820 1556
Cuts at: 11045 11317 11671 107
Size: 272 354 2619
Fragments arranged by size:
2835 2619 1556 1260 820 767 683 666
525 415 354 350 315 306 272 237
129 39 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 606 1693 2449 2683 3033 3994 5055 5926 7666
Size: 1087 756 234 350 961 1061 871 1740
Cuts at: 7666 7802 8482 8514 8527 11766 11899 12358 12567
Size: 136 680 32 13 3239 133 459 209
Cuts at: 12567 606
Size: 2222
Fragments arranged by size:
3239 2222 1740 1087 1061 961 871 756
680 459 350 234 209 136 133 32
13
TatI w'GTAC_w
Cuts at: 410 521 761 3545 4233 5183 8456 9251 13698
Size: 111 240 2784 688 950 3273 795 4447
Cuts at: 13698 410
Size: 895
Fragments arranged by size:
4447 3273 2784 950 895 795 688 240
111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 782 814 3192 3224 3283 3315 3638
Size: 32 278 32 2378 32 59 32 323
Cuts at: 3638 3670 4604 4636 7738 7770 8140 8172 8754
Size: 32 934 32 3102 32 370 32 582
Cuts at: 8754 8786 10375 10407 13700 13732 472
Size: 32 1589 32 3293 32 923
Fragments arranged by size:
3293 3102 2378 1589 934 923 582 370
323 278 59 32 32 32 32 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1649 8728 11980 1649
Size: 7079 3252 3852
Fragments arranged by size:
7079 3852 3252
XbaI T'CTAG_A
Cuts at: 11214 11214
Size: 14183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 912 1251 1255 1746 3742 4047 4939 5380 6957
Size: 339 4 491 1996 305 892 441 1577
Cuts at: 6957 7623 7962 11678 912
Size: 666 339 3716 3417
Fragments arranged by size:
3716 3417 1996 1577 892 666 491 441
339 339 305 4
XhoI C'TCGA_G
Cuts at: 2794 2794
Size: 14183
XmaI C'CCGG_G
Cuts at: 155 3572 3713 6510 7091 7157 7367 8285 10008
Size: 3417 141 2797 581 66 210 918 1723
Cuts at: 10008 10439 11203 155
Size: 431 764 3135
Fragments arranged by size:
3417 3135 2797 1723 918 764 581 431
210 141 66
ZraI GAC'GTC
Cuts at: 820 3396 5664 7632 820
Size: 2576 2268 1968 7371
Fragments arranged by size:
7371 2576 2268 1968
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AflII AvrII BfrBI BsiWI BstZ17I FspAI HindIII HpaI
NdeI NruI NsiI PacI PsiI PsrI SnaBI SspI
SwaI XmnI