Restriction map for
pF1KA0531
(Circular) (Six-base) MAPSORT of: pF1KA0531.seq Check: 5287 from: 1 to: 5984
pF1KA0531 5984 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 3022 3022
Size: 5984
Acc65I G'GTAC_C
Cuts at: 3000 3000
Size: 5984
AclI AA'CG_TT
Cuts at: 5824 5824
Size: 5984
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1462 2216 2776 3809 4241 4988 5256 955
Size: 507 754 560 1033 432 747 268 1683
Fragments arranged by size:
1683 1033 754 747 560 507 432 268
AfeI AGC'GCT
Cuts at: 2794 2794
Size: 5984
AflIII A'CryG_T
Cuts at: 681 933 1519 3336 4440 5417 681
Size: 252 586 1817 1104 977 1248
Fragments arranged by size:
1817 1248 1104 977 586 252
AgeI A'CCGG_T
Cuts at: 4372 4372
Size: 5984
AlwNI CAG_nnn'CTG
Cuts at: 1102 1194 1578 2331 2499 2532 3289 4856 1102
Size: 92 384 753 168 33 757 1567 2230
Fragments arranged by size:
2230 1567 757 753 384 168 92 33
ApaI G_GGCC'C
Cuts at: 2859 2859
Size: 5984
ApaLI G'TGCA_C
Cuts at: 612 4754 612
Size: 4142 1842
ApoI r'AATT_y
Cuts at: 190 202 1761 2606 2958 2988 5337 5593 190
Size: 12 1559 845 352 30 2349 256 581
Fragments arranged by size:
2349 1559 845 581 352 256 30 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5984
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
AvaI C'yCGr_G
Cuts at: 1634 1930 2540 3004 1634
Size: 296 610 464 4614
Fragments arranged by size:
4614 610 464 296
BamHI G'GATC_C
Cuts at: 114 3009 114
Size: 2895 3089
BanI G'GyrC_C
Cuts at: 835 1804 3000 3414 3662 3697 835
Size: 969 1196 414 248 35 3122
Fragments arranged by size:
3122 1196 969 414 248 35
BanII G_rGCy'C
Cuts at: 637 2127 2859 2998 4028 637
Size: 1490 732 139 1030 2593
Fragments arranged by size:
2593 1490 1030 732 139
BbeI G_GCGC'C
Cuts at: 3418 3666 3418
Size: 248 5736
BbsI GAAGACnn'nnnn_
Cuts at: 243 1023 2459 243
Size: 780 1436 3768
Fragments arranged by size:
3768 1436 780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1911 1945 2491 2525 1911
Size: 34 546 34 5370
Fragments arranged by size:
5370 546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 564 3882 4643 5984 564
Size: 3318 761 1341 564
Fragments arranged by size:
3318 1341 761 564
BclI T'GATC_A
Cuts at: 3504 3504
Size: 5984
BfrBI ATG'CAT
Cuts at: 301 301
Size: 5984
BglI GCCn_nnn'nGGC
Cuts at: 2833 2854 2833
Size: 21 5963
BglII A'GATC_T
Cuts at: 518 1367 1776 3499 5536 518
Size: 849 409 1723 2037 966
Fragments arranged by size:
2037 1723 966 849 409
BlpI GC'TnA_GC
Cuts at: 1705 1723 2098 3096 1705
Size: 18 375 998 4593
Fragments arranged by size:
4593 998 375 18
Bme1580I G_kGCm'C
Cuts at: 616 2859 3609 3702 4758 616
Size: 2243 750 93 1056 1842
Fragments arranged by size:
2243 1842 1056 750 93
BmgBI CAC'GTC
Cuts at: 1522 1522
Size: 5984
BmrI ACTGGGnnnn_n'
Cuts at: 3196 3277 3612 5756 3196
Size: 81 335 2144 3424
Fragments arranged by size:
3424 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1386 2473 4351 1386
Size: 1087 1878 3019
Fragments arranged by size:
3019 1878 1087
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1216 1297 1356 1510 1555 1600 2236 2488
Size: 708 81 59 154 45 45 636 252
Cuts at: 2488 2650 2722 2764 508
Size: 162 72 42 3728
Fragments arranged by size:
3728 708 636 252 162 154 81 72
59 45 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3160 4039 4531 4829 3160
Size: 879 492 298 4315
Fragments arranged by size:
4315 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 211 1039 5688 211
Size: 828 4649 507
Fragments arranged by size:
4649 828 507
BsaAI yAC'GTr
Cuts at: 684 3337 684
Size: 2653 3331
BsaBI GATnn'nnATC
Cuts at: 6 2321 3498 5535 6
Size: 2315 1177 2037 455
Fragments arranged by size:
2315 2037 1177 455
BsaHI Gr'CG_yC
Cuts at: 3415 3663 4365 5861 3415
Size: 248 702 1496 3538
Fragments arranged by size:
3538 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3173 3694 4372 4646 4793 5204 3173
Size: 521 678 274 147 411 3953
Fragments arranged by size:
3953 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1217 1247 2464 2494 1217
Size: 30 1217 30 4707
Fragments arranged by size:
4707 1217 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1357 1378 1525 1597 1864 1867 2082 2299 2485
Size: 21 147 72 267 3 215 217 186
Cuts at: 2485 2957 1357
Size: 472 4384
Fragments arranged by size:
4384 472 267 217 215 186 147 72
21 3
BseYI C'CCAG_C
Cuts at: 416 808 2009 3409 4167 4744 5240 416
Size: 392 1201 1400 758 577 496 1160
Fragments arranged by size:
1400 1201 1160 758 577 496 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2485 2485
Size: 5984
BsiEI CG_ry'CG
Cuts at: 104 2921 3526 3572 4780 104
Size: 2817 605 46 1208 1308
Fragments arranged by size:
2817 1308 1208 605 46
BsiHKAI G_wGCw'C
Cuts at: 616 2998 3776 3966 4758 616
Size: 2382 778 190 792 1842
Fragments arranged by size:
2382 1842 792 778 190
BsmI GAATG_Cn'
Cuts at: 130 1971 130
Size: 1841 4143
BsmBI CGTCTCn'nnnn_
Cuts at: 1528 2267 1528
Size: 739 5245
Bsp1286I G_dGCh'C
Cuts at: 616 637 2127 2859 2998 3609 3702 3776 3966
Size: 21 1490 732 139 611 93 74 190
Cuts at: 3966 4028 4758 616
Size: 62 730 1842
Fragments arranged by size:
1842 1490 732 730 611 190 139 93
74 62 21
BspEI T'CCGG_A
Cuts at: 5204 5204
Size: 5984
BspHI T'CATG_A
Cuts at: 576 5160 576
Size: 4584 1400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 279 2687 3034 3248 3550 3931 279
Size: 2408 347 214 302 381 2332
Fragments arranged by size:
2408 2332 381 347 302 214
BsrBI CCG'CTC
Cuts at: 50 621 1977 4276 4330 5814 5979 50
Size: 571 1356 2299 54 1484 165 55
Fragments arranged by size:
2299 1484 1356 571 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3896 3896
Size: 5984
BsrFI r'CCGG_y
Cuts at: 146 3982 4163 4372 146
Size: 3836 181 209 1758
Fragments arranged by size:
3836 1758 209 181
BsrGI T'GTAC_A
Cuts at: 1874 1874
Size: 5984
BssHII G'CGCG_C
Cuts at: 2750 2750
Size: 5984
BssSI C'ACGA_G
Cuts at: 4255 4613 4255
Size: 358 5626
BstAPI GCAn_nnn'nTGC
Cuts at: 2499 3361 2499
Size: 862 5122
BstBI TT'CG_AA
Cuts at: 4345 4345
Size: 5984
BstXI CCAn_nnnn'nTGG
Cuts at: 2844 2844
Size: 5984
BstYI r'GATC_y
Cuts at: 1 114 173 518 1367 1754 1776 2529 3009
Size: 113 59 345 849 387 22 753 480
Cuts at: 3009 3476 3499 3834 4080 5092 5178 5190 5536
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5536 1
Size: 449
Fragments arranged by size:
1012 849 753 480 467 449 387 346
345 335 246 113 86 59 23 22
12
Bsu36I CC'TnA_GG
Cuts at: 638 1238 638
Size: 600 5384
BtgI C'CryG_G
Cuts at: 107 180 221 392 473 1862 2720 107
Size: 73 41 171 81 1389 858 3371
Fragments arranged by size:
3371 1389 858 171 81 73 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 227 3934 4115 4278 227
Size: 3707 181 163 1933
Fragments arranged by size:
3707 1933 181 163
BtsI GCAGTG_nn'
Cuts at: 2497 2592 3227 2497
Size: 95 635 5254
Fragments arranged by size:
5254 635 95
DraI TTT'AAA
Cuts at: 207 2983 207
Size: 2776 3208
DrdI GACnn_nn'nnGTC
Cuts at: 3690 4548 3690
Size: 858 5126
EaeI y'GGCC_r
Cuts at: 2846 2918 3569 3743 4134 4161 5506 2846
Size: 72 651 174 391 27 1345 3324
Fragments arranged by size:
3324 1345 651 391 174 72 27
EagI C'GGCC_G
Cuts at: 2918 3569 2918
Size: 651 5333
EarI CTCTTCn'nnn_
Cuts at: 1482 2059 2075 2099 2958 4007 4217 1482
Size: 577 16 24 859 1049 210 3249
Fragments arranged by size:
3249 1049 859 577 210 24 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2740 2875 4192 4502 4648 5794 127
Size: 2613 135 1317 310 146 1146 317
Fragments arranged by size:
2613 1317 1146 317 310 146 135
EcoICRI GAG'CTC
Cuts at: 2996 2996
Size: 5984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 955 1216 1297 1356 1462 1510 1555 1600
Size: 447 261 81 59 106 48 45 45
Cuts at: 1600 2216 2236 2488 2650 2722 2764 2776 3809
Size: 616 20 252 162 72 42 12 1033
Cuts at: 3809 4241 4988 5256 508
Size: 432 747 268 1236
Fragments arranged by size:
1236 1033 747 616 447 432 268 261
252 162 106 81 72 59 48 45
45 42 20 12
EcoO109I rG'GnC_Cy
Cuts at: 1575 2855 3123 1575
Size: 1280 268 4436
Fragments arranged by size:
4436 1280 268
EcoRI G'AATT_C
Cuts at: 2988 2988
Size: 5984
EcoRV GAT'ATC
Cuts at: 453 453
Size: 5984
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 346 582 4003 346
Size: 236 3421 2327
Fragments arranged by size:
3421 2327 236
FspI TGC'GCA
Cuts at: 3765 3765
Size: 5984
HaeII r_GCGC'y
Cuts at: 1940 2215 2796 3418 3666 4688 5667 1940
Size: 275 581 622 248 1022 979 2257
Fragments arranged by size:
2257 1022 979 622 581 275 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1036 1068 1359 1391 1443 1475 1594 1626 1888
Size: 32 291 32 52 32 119 32 262
Cuts at: 1888 1920 2016 2048 2464 2496 3826 3856 3858
Size: 32 96 32 416 32 1330 30 2
Cuts at: 3858 3888 1036
Size: 30 3132
Fragments arranged by size:
3132 1330 416 291 262 119 96 52
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3023 3023
Size: 5984
HindIII A'AGCT_T
Cuts at: 2350 2350
Size: 5984
KasI G'GCGC_C
Cuts at: 3414 3662 3414
Size: 248 5736
KpnI G_GTAC'C
Cuts at: 3004 3004
Size: 5984
MluI A'CGCG_T
Cuts at: 5417 5417
Size: 5984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1976 2543 2938 4655 4839 1436
Size: 540 567 395 1717 184 2581
Fragments arranged by size:
2581 1717 567 540 395 184
MscI TGG'CCA
Cuts at: 2848 3745 2848
Size: 897 5087
MslI CAynn'nnrTG
Cuts at: 136 1060 3973 4100 136
Size: 924 2913 127 2020
Fragments arranged by size:
2913 2020 924 127
MspA1I CmG'CkG
Cuts at: 182 1428 1650 1998 2259 2658 3095 3409 3769
Size: 1246 222 348 261 399 437 314 360
Cuts at: 3769 4782 5027 182
Size: 1013 245 1139
Fragments arranged by size:
1246 1139 1013 437 399 360 348 314
261 245 222
NaeI GCC'GGC
Cuts at: 4165 4165
Size: 5984
NarI GG'CG_CC
Cuts at: 3415 3663 3415
Size: 248 5736
NcoI C'CATG_G
Cuts at: 107 392 473 1862 107
Size: 285 81 1389 4229
Fragments arranged by size:
4229 1389 285 81
NdeI CA'TA_TG
Cuts at: 246 246
Size: 5984
NgoMIV G'CCGG_C
Cuts at: 4163 4163
Size: 5984
NsiI A_TGCA'T
Cuts at: 303 303
Size: 5984
NspI r_CATG'y
Cuts at: 305 937 2049 3037 3537 4444 305
Size: 632 1112 988 500 907 1845
Fragments arranged by size:
1845 1112 988 907 632 500
PciI A'CATG_T
Cuts at: 933 4440 933
Size: 3507 2477
PflMI CCAn_nnn'nTGG
Cuts at: 393 423 1194 1578 393
Size: 30 771 384 4799
Fragments arranged by size:
4799 771 384 30
PfoI T'CCnGG_A
Cuts at: 2153 2525 2663 2153
Size: 372 138 5474
Fragments arranged by size:
5474 372 138
PmeI GTTT'AAAC
Cuts at: 2983 2983
Size: 5984
PmlI CAC'GTG
Cuts at: 684 684
Size: 5984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5123 5155 5123
Size: 32 5952
PsiI TTA'TAA
Cuts at: 341 2366 341
Size: 2025 3959
PspOMI G'GGCC_C
Cuts at: 2855 2855
Size: 5984
PstI C_TGCA'G
Cuts at: 997 3031 3716 997
Size: 2034 685 3265
Fragments arranged by size:
3265 2034 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5984
PvuII CAG'CTG
Cuts at: 1428 1650 1998 2259 2658 3409 3769 1428
Size: 222 348 261 399 751 360 3643
Fragments arranged by size:
3643 751 399 360 348 261 222
RsrII CG'GwC_CG
Cuts at: 4179 4179
Size: 5984
SacI G_AGCT'C
Cuts at: 2998 2998
Size: 5984
SacII CC_GC'GG
Cuts at: 183 183
Size: 5984
SalI G'TCGA_C
Cuts at: 3021 3021
Size: 5984
SapI GCTCTTCn'nnn_
Cuts at: 2099 2958 4007 4217 2099
Size: 859 1049 210 3866
Fragments arranged by size:
3866 1049 859 210
SbfI CC_TGCA'GG
Cuts at: 3031 3031
Size: 5984
ScaI AGT'ACT
Cuts at: 5501 5501
Size: 5984
SfcI C'TryA_G
Cuts at: 33 993 1337 1380 3027 3712 4705 4896 33
Size: 960 344 43 1647 685 993 191 1121
Fragments arranged by size:
1647 1121 993 960 685 344 191 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 2833 2854 2833
Size: 21 5963
SfoI GGC'GCC
Cuts at: 3416 3664 3416
Size: 248 5736
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5984
SmaI CCC'GGG
Cuts at: 1636 3006 1636
Size: 1370 4614
SmlI C'TyrA_G
Cuts at: 1930 3139 4054 4546 4808 1930
Size: 1209 915 492 262 3106
Fragments arranged by size:
3106 1209 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5984
SphI G_CATG'C
Cuts at: 3037 3537 3037
Size: 500 5484
SspI AAT'ATT
Cuts at: 744 1311 744
Size: 567 5417
StyI C'CwwG_G
Cuts at: 107 392 473 1259 1547 1862 2765 3118 3472
Size: 285 81 786 288 315 903 353 354
Cuts at: 3472 107
Size: 2619
Fragments arranged by size:
2619 903 786 354 353 315 288 285
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 973 3567 3700 4159 4368 973
Size: 2594 133 459 209 2589
Fragments arranged by size:
2594 2589 459 209 133
TatI w'GTAC_w
Cuts at: 1874 5499 1874
Size: 3625 2359
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1217 1249 5501 5533 1217
Size: 32 4252 32 1668
Fragments arranged by size:
4252 1668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1050 2478 3781 1050
Size: 1428 1303 3253
Fragments arranged by size:
3253 1428 1303
XbaI T'CTAG_A
Cuts at: 715 2482 3015 715
Size: 1767 533 3684
Fragments arranged by size:
3684 1767 533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 564 3479 564
Size: 2915 3069
XhoI C'TCGA_G
Cuts at: 1930 1930
Size: 5984
XmaI C'CCGG_G
Cuts at: 1634 3004 1634
Size: 1370 4614
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BmtI Bpu10I BsiWI BstEII BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PpuMI PshAI PspXI
PsrI SanDI SexAI SgrAI SnaBI SrfI StuI SwaI
XmnI ZraI