Restriction map for
pF1KA0528
(Circular) (Six-base) MAPSORT of: pF1KA0528.seq Check: 1372 from: 1 to: 6113
pF1KA0528 6113 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 491 1011 3151 491
Size: 520 2140 3453
Fragments arranged by size:
3453 2140 520
Acc65I G'GTAC_C
Cuts at: 3129 3129
Size: 6113
AclI AA'CG_TT
Cuts at: 5953 5953
Size: 6113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 2039 2195 2270 2468 2891 3110 3938 4370
Size: 1897 156 75 198 423 219 828 432
Cuts at: 4370 5117 5385 142
Size: 747 268 870
Fragments arranged by size:
1897 870 828 747 432 423 268 219
198 156 75
AflII C'TTAA_G
Cuts at: 238 238
Size: 6113
AflIII A'CryG_T
Cuts at: 1194 1486 2795 3465 4569 5546 1194
Size: 292 1309 670 1104 977 1761
Fragments arranged by size:
1761 1309 1104 977 670 292
AgeI A'CCGG_T
Cuts at: 4501 4501
Size: 6113
AleI CACnn'nnGTG
Cuts at: 1855 1855
Size: 6113
AlwNI CAG_nnn'CTG
Cuts at: 1408 3418 4985 1408
Size: 2010 1567 2536
Fragments arranged by size:
2536 2010 1567
ApaI G_GGCC'C
Cuts at: 1097 1097
Size: 6113
ApaLI G'TGCA_C
Cuts at: 1214 4883 1214
Size: 3669 2444
ApoI r'AATT_y
Cuts at: 202 277 541 650 1147 1476 1802 2364 2427
Size: 75 264 109 497 329 326 562 63
Cuts at: 2427 2679 2712 3117 5466 5722 202
Size: 252 33 405 2349 256 593
Fragments arranged by size:
2349 593 562 497 405 329 326 264
256 252 109 75 63 33
AseI AT'TA_AT
Cuts at: 20 2458 20
Size: 2438 3675
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6113
AvaI C'yCGr_G
Cuts at: 1268 3133 1268
Size: 1865 4248
AvrII C'CTAG_G
Cuts at: 2050 2050
Size: 6113
BamHI G'GATC_C
Cuts at: 3138 3138
Size: 6113
BanI G'GyrC_C
Cuts at: 143 1438 3129 3543 3791 3826 143
Size: 1295 1691 414 248 35 2430
Fragments arranged by size:
2430 1691 1295 414 248 35
BanII G_rGCy'C
Cuts at: 1097 2895 3127 4157 1097
Size: 1798 232 1030 3053
Fragments arranged by size:
3053 1798 1030 232
BbeI G_GCGC'C
Cuts at: 147 3547 3795 147
Size: 3400 248 2465
Fragments arranged by size:
3400 2465 248
BbsI GAAGACnn'nnnn_
Cuts at: 302 629 1912 2943 302
Size: 327 1283 1031 3472
Fragments arranged by size:
3472 1283 1031 327
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6079
BciVI GTATCCnnnnn_n'
Cuts at: 469 1252 4011 4772 6113 469
Size: 783 2759 761 1341 469
Fragments arranged by size:
2759 1341 783 761 469
BclI T'GATC_A
Cuts at: 3633 3633
Size: 6113
BfrBI ATG'CAT
Cuts at: 1276 1276
Size: 6113
BglII A'GATC_T
Cuts at: 2074 3628 5665 2074
Size: 1554 2037 2522
Fragments arranged by size:
2522 2037 1554
BlpI GC'TnA_GC
Cuts at: 1839 3225 1839
Size: 1386 4727
Bme1580I G_kGCm'C
Cuts at: 1097 1218 3738 3831 4887 1097
Size: 121 2520 93 1056 2323
Fragments arranged by size:
2520 2323 1056 121 93
BmrI ACTGGGnnnn_n'
Cuts at: 3325 3406 3741 5885 3325
Size: 81 335 2144 3553
Fragments arranged by size:
3553 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4480 4480
Size: 6113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1991 2780 811
Size: 1180 789 4144
Fragments arranged by size:
4144 1180 789
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2046 2356 3289 4168 4660 4958 2046
Size: 310 933 879 492 298 3201
Fragments arranged by size:
3201 933 879 492 310 298
BsaI GGTCTCn'nnnn_
Cuts at: 5817 5817
Size: 6113
BsaAI yAC'GTr
Cuts at: 3466 3466
Size: 6113
BsaBI GATnn'nnATC
Cuts at: 6 386 509 929 2079 3627 5664 6
Size: 380 123 420 1150 1548 2037 455
Fragments arranged by size:
2037 1548 1150 455 420 380 123
BsaHI Gr'CG_yC
Cuts at: 144 3544 3792 4494 5990 144
Size: 3400 248 702 1496 267
Fragments arranged by size:
3400 1496 702 267 248
BsaWI w'CCGG_w
Cuts at: 2684 3302 3823 4501 4775 4922 5333 2684
Size: 618 521 678 274 147 411 3464
Fragments arranged by size:
3464 678 618 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 784 814 784
Size: 30 6083
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2195 2852 2195
Size: 657 5456
BseYI C'CCAG_C
Cuts at: 858 1900 3538 4296 4873 5369 858
Size: 1042 1638 758 577 496 1602
Fragments arranged by size:
1638 1602 1042 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3655 3701 4909 104
Size: 3551 46 1208 1308
Fragments arranged by size:
3551 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1218 2895 3127 3905 4095 4887 1218
Size: 1677 232 778 190 792 2444
Fragments arranged by size:
2444 1677 792 778 232 190
BsmI GAATG_Cn'
Cuts at: 865 1788 2922 865
Size: 923 1134 4056
Fragments arranged by size:
4056 1134 923
Bsp1286I G_dGCh'C
Cuts at: 1097 1218 2895 3127 3738 3831 3905 4095 4157
Size: 121 1677 232 611 93 74 190 62
Cuts at: 4157 4887 1097
Size: 730 2323
Fragments arranged by size:
2323 1677 730 611 232 190 121 93
74 62
BspEI T'CCGG_A
Cuts at: 2684 5333 2684
Size: 2649 3464
BspHI T'CATG_A
Cuts at: 5289 5289
Size: 6113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3163 3377 3679 4060 3163
Size: 214 302 381 5216
Fragments arranged by size:
5216 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4405 4459 5943 6108 50
Size: 4355 54 1484 165 55
Fragments arranged by size:
4355 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2542 2888 4025 2542
Size: 346 1137 4630
Fragments arranged by size:
4630 1137 346
BsrFI r'CCGG_y
Cuts at: 4111 4292 4501 4111
Size: 181 209 5723
Fragments arranged by size:
5723 209 181
BsrGI T'GTAC_A
Cuts at: 374 1191 374
Size: 817 5296
BssSI C'ACGA_G
Cuts at: 4384 4742 4384
Size: 358 5755
BstAPI GCAn_nnn'nTGC
Cuts at: 358 3490 358
Size: 3132 2981
BstBI TT'CG_AA
Cuts at: 4474 4474
Size: 6113
BstYI r'GATC_y
Cuts at: 1 924 2074 3138 3605 3628 3963 4209 5221
Size: 923 1150 1064 467 23 335 246 1012
Cuts at: 5221 5307 5319 5665 1
Size: 86 12 346 449
Fragments arranged by size:
1150 1064 1012 923 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 462 1442 2168 462
Size: 980 726 4407
Fragments arranged by size:
4407 980 726
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4063 4244 4407 4063
Size: 181 163 5769
Fragments arranged by size:
5769 181 163
BtsI GCAGTG_nn'
Cuts at: 2950 3017 3356 2950
Size: 67 339 5707
Fragments arranged by size:
5707 339 67
ClaI AT'CG_AT
Cuts at: 513 513
Size: 6113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2648 2683 2648
Size: 35 6078
DraI TTT'AAA
Cuts at: 222 276 509 1101 2388 3112 222
Size: 54 233 592 1287 724 3223
Fragments arranged by size:
3223 1287 724 592 233 54
DraIII CAC_nnn'GTG
Cuts at: 1492 2707 1492
Size: 1215 4898
DrdI GACnn_nn'nnGTC
Cuts at: 3819 4677 3819
Size: 858 5255
EaeI y'GGCC_r
Cuts at: 137 3698 3872 4263 4290 5635 137
Size: 3561 174 391 27 1345 615
Fragments arranged by size:
3561 1345 615 391 174 27
EagI C'GGCC_G
Cuts at: 3698 3698
Size: 6113
EarI CTCTTCn'nnn_
Cuts at: 502 1026 1367 2015 2828 4136 4346 502
Size: 524 341 648 813 1308 210 2269
Fragments arranged by size:
2269 1308 813 648 524 341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4321 4631 4777 5923 4321
Size: 310 146 1146 4511
Fragments arranged by size:
4511 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2893 3125 2893
Size: 232 5881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 811 1991 2039 2195 2270 2468 2780 2891
Size: 669 1180 48 156 75 198 312 111
Cuts at: 2891 3110 3938 4370 5117 5385 142
Size: 219 828 432 747 268 870
Fragments arranged by size:
1180 870 828 747 669 432 312 268
219 198 156 111 75 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2210
Size: 6113
EcoO109I rG'GnC_Cy
Cuts at: 952 1093 1094 3252 952
Size: 141 1 2158 3813
Fragments arranged by size:
3813 2158 141 1
EcoRI G'AATT_C
Cuts at: 650 1802 3117 650
Size: 1152 1315 3646
Fragments arranged by size:
3646 1315 1152
EcoRV GAT'ATC
Cuts at: 2015 2015
Size: 6113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 747 1660 4132 747
Size: 913 2472 2728
Fragments arranged by size:
2728 2472 913
FspI TGC'GCA
Cuts at: 3894 3894
Size: 6113
HaeII r_GCGC'y
Cuts at: 147 3547 3795 4817 5796 147
Size: 3400 248 1022 979 464
Fragments arranged by size:
3400 1022 979 464 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 674 706 784 816 2024 2056 2387 2419 2727
Size: 32 78 32 1208 32 331 32 308
Cuts at: 2727 2759 2805 2837 3955 3985 3987 4017 674
Size: 32 46 32 1118 30 2 30 2770
Fragments arranged by size:
2770 1208 1118 331 308 78 46 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1012 3152 1012
Size: 2140 3973
HindIII A'AGCT_T
Cuts at: 1228 1228
Size: 6113
KasI G'GCGC_C
Cuts at: 143 3543 3791 143
Size: 3400 248 2465
Fragments arranged by size:
3400 2465 248
KpnI G_GTAC'C
Cuts at: 3133 3133
Size: 6113
MfeI C'AATT_G
Cuts at: 1970 1970
Size: 6113
MluI A'CGCG_T
Cuts at: 5546 5546
Size: 6113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1115 4784 4968 1115
Size: 3669 184 2260
Fragments arranged by size:
3669 2260 184
MscI TGG'CCA
Cuts at: 3874 3874
Size: 6113
MslI CAynn'nnrTG
Cuts at: 1855 4102 4229 1855
Size: 2247 127 3739
Fragments arranged by size:
3739 2247 127
MspA1I CmG'CkG
Cuts at: 1405 3224 3538 3898 4911 5156 1405
Size: 1819 314 360 1013 245 2362
Fragments arranged by size:
2362 1819 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4294 4294
Size: 6113
NarI GG'CG_CC
Cuts at: 144 3544 3792 144
Size: 3400 248 2465
Fragments arranged by size:
3400 2465 248
NcoI C'CATG_G
Cuts at: 2168 2168
Size: 6113
NdeI CA'TA_TG
Cuts at: 1512 1926 1946 1512
Size: 414 20 5679
Fragments arranged by size:
5679 414 20
NgoMIV G'CCGG_C
Cuts at: 4292 4292
Size: 6113
NsiI A_TGCA'T
Cuts at: 1278 1278
Size: 6113
NspI r_CATG'y
Cuts at: 881 1315 2799 3166 3666 4573 881
Size: 434 1484 367 500 907 2421
Fragments arranged by size:
2421 1484 907 500 434 367
PciI A'CATG_T
Cuts at: 2795 4569 2795
Size: 1774 4339
PflMI CCAn_nnn'nTGG
Cuts at: 1882 1882
Size: 6113
PfoI T'CCnGG_A
Cuts at: 1179 2757 1179
Size: 1578 4535
PmeI GTTT'AAAC
Cuts at: 3112 3112
Size: 6113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5252 5284 5252
Size: 32 6081
PpuMI rG'GwC_Cy
Cuts at: 952 952
Size: 6113
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 6113
PspOMI G'GGCC_C
Cuts at: 1093 1093
Size: 6113
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 819 851 819
Size: 32 6081
PstI C_TGCA'G
Cuts at: 719 1433 1567 3160 3845 719
Size: 714 134 1593 685 2987
Fragments arranged by size:
2987 1593 714 685 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6113
PvuII CAG'CTG
Cuts at: 3538 3898 3538
Size: 360 5753
RsrII CG'GwC_CG
Cuts at: 4308 4308
Size: 6113
SacI G_AGCT'C
Cuts at: 2895 3127 2895
Size: 232 5881
SalI G'TCGA_C
Cuts at: 1010 3150 1010
Size: 2140 3973
SapI GCTCTTCn'nnn_
Cuts at: 4136 4346 4136
Size: 210 5903
SbfI CC_TGCA'GG
Cuts at: 3160 3160
Size: 6113
ScaI AGT'ACT
Cuts at: 740 5630 740
Size: 4890 1223
SfcI C'TryA_G
Cuts at: 33 573 715 1005 1337 1347 1429 1563 1601
Size: 540 142 290 332 10 82 134 38
Cuts at: 1601 1700 3156 3841 4834 5025 33
Size: 99 1456 685 993 191 1121
Fragments arranged by size:
1456 1121 993 685 540 332 290 191
142 134 99 82 38 10
SfoI GGC'GCC
Cuts at: 145 3545 3793 145
Size: 3400 248 2465
Fragments arranged by size:
3400 2465 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6113
SmaI CCC'GGG
Cuts at: 3135 3135
Size: 6113
SmlI C'TyrA_G
Cuts at: 238 1268 2061 2371 3268 4183 4675 4937 238
Size: 1030 793 310 897 915 492 262 1414
Fragments arranged by size:
1414 1030 915 897 793 492 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6113
SphI G_CATG'C
Cuts at: 3166 3666 3166
Size: 500 5613
StyI C'CwwG_G
Cuts at: 661 2050 2168 3247 3601 661
Size: 1389 118 1079 354 3173
Fragments arranged by size:
3173 1389 1079 354 118
SwaI ATTT'AAAT
Cuts at: 509 509
Size: 6113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1065 1807 2052 2659 3696 3829 4288 4497 1065
Size: 742 245 607 1037 133 459 209 2681
Fragments arranged by size:
2681 1037 742 607 459 245 209 133
TatI w'GTAC_w
Cuts at: 374 738 835 1191 1410 2381 3085 5628 374
Size: 364 97 356 219 971 704 2543 859
Fragments arranged by size:
2543 971 859 704 364 356 219 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5630 5662 5630
Size: 32 6081
Tth111I GACn'n_nGTC
Cuts at: 3910 3910
Size: 6113
XbaI T'CTAG_A
Cuts at: 3144 3144
Size: 6113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 635 1059 3608 635
Size: 424 2549 3140
Fragments arranged by size:
3140 2549 424
XhoI C'TCGA_G
Cuts at: 1268 1268
Size: 6113
XmaI C'CCGG_G
Cuts at: 3133 3133
Size: 6113
XmnI GAAnn'nnTTC
Cuts at: 2459 2459
Size: 6113
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI BaeI
BbvCI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I FseI FspAI HpaI
NheI NotI NruI PacI PasI PmlI PshAI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI ZraI