Restriction map for
pF1KA0481
(Circular) (Six-base) MAPSORT of: pF1KA0481.seq Check: 4089 from: 1 to: 5240
pF1KA0481 5240 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 197 704 989 1080 1977 197
Size: 507 285 91 897 3460
Fragments arranged by size:
3460 897 507 285 91
AatII G_ACGT'C
Cuts at: 412 412
Size: 5240
AccI GT'mk_AC
Cuts at: 2278 2278
Size: 5240
Acc65I G'GTAC_C
Cuts at: 2256 2256
Size: 5240
AclI AA'CG_TT
Cuts at: 5080 5080
Size: 5240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 379 1018 1210 1279 1462 1747 1876 3065
Size: 143 639 192 69 183 285 129 1189
Cuts at: 3065 3497 3850 4512 236
Size: 432 353 662 964
Fragments arranged by size:
1189 964 662 639 432 353 285 192
183 143 129 69
AfeI AGC'GCT
Cuts at: 481 1783 481
Size: 1302 3938
AflIII A'CryG_T
Cuts at: 812 2219 2592 4392 4673 812
Size: 1407 373 1800 281 1379
Fragments arranged by size:
1800 1407 1379 373 281
AleI CACnn'nnGTG
Cuts at: 1366 1366
Size: 5240
AlwNI CAG_nnn'CTG
Cuts at: 327 1257 1770 1866 2545 3983 327
Size: 930 513 96 679 1438 1584
Fragments arranged by size:
1584 1438 930 679 513 96
ApaI G_GGCC'C
Cuts at: 1214 1466 1214
Size: 252 4988
ApaLI G'TGCA_C
Cuts at: 4078 4078
Size: 5240
ApoI r'AATT_y
Cuts at: 2244 4593 4849 2244
Size: 2349 256 2635
Fragments arranged by size:
2635 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5240
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5240
AvaI C'yCGr_G
Cuts at: 389 1382 1481 1949 2260 389
Size: 993 99 468 311 3369
Fragments arranged by size:
3369 993 468 311 99
BamHI G'GATC_C
Cuts at: 2265 2265
Size: 5240
BanI G'GyrC_C
Cuts at: 273 598 942 1184 1270 2256 2670 2918 2953
Size: 325 344 242 86 986 414 248 35
Cuts at: 2953 273
Size: 2560
Fragments arranged by size:
2560 986 414 344 325 248 242 86
35
BanII G_rGCy'C
Cuts at: 340 677 1214 1466 1618 2254 3284 340
Size: 337 537 252 152 636 1030 2296
Fragments arranged by size:
2296 1030 636 537 337 252 152
BbeI G_GCGC'C
Cuts at: 277 602 1188 1274 2674 2922 277
Size: 325 586 86 1400 248 2595
Fragments arranged by size:
2595 1400 586 325 248 86
BbsI GAAGACnn'nnnn_
Cuts at: 1727 1735 2131 1727
Size: 8 396 4836
Fragments arranged by size:
4836 396 8
BbvCI CC'TCA_GC
Cuts at: 827 827
Size: 5240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1015 1049 1015
Size: 34 5206
BciVI GTATCCnnnnn_n'
Cuts at: 3138 4194 5240 3138
Size: 1056 1046 3138
Fragments arranged by size:
3138 1056 1046
BclI T'GATC_A
Cuts at: 2760 2760
Size: 5240
BfrBI ATG'CAT
Cuts at: 495 495
Size: 5240
BglI GCCn_nnn'nGGC
Cuts at: 537 1218 1340 537
Size: 681 122 4437
Fragments arranged by size:
4437 681 122
BglII A'GATC_T
Cuts at: 2755 4792 2755
Size: 2037 3203
BlpI GC'TnA_GC
Cuts at: 1563 2352 1563
Size: 789 4451
Bme1580I G_kGCm'C
Cuts at: 935 945 1214 1466 1488 1914 2865 2958 4082
Size: 10 269 252 22 426 951 93 1124
Cuts at: 4082 935
Size: 2093
Fragments arranged by size:
2093 1124 951 426 269 252 93 22
10
BmgBI CAC'GTC
Cuts at: 930 1233 1266 930
Size: 303 33 4904
Fragments arranged by size:
4904 303 33
BmrI ACTGGGnnnn_n'
Cuts at: 1758 2199 2452 2533 2868 5012 1758
Size: 441 253 81 335 2144 1986
Fragments arranged by size:
2144 1986 441 335 253 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3607 3607
Size: 5240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1189 1497 1530 1726 1822 1984 2020 2233
Size: 498 308 33 196 96 162 36 213
Cuts at: 2233 691
Size: 3698
Fragments arranged by size:
3698 498 308 213 196 162 96 36
33
Bpu10I CC'TnA_GC
Cuts at: 827 880 827
Size: 53 5187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2416 3295 4009 4307 2416
Size: 879 714 298 3349
Fragments arranged by size:
3349 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 211 392 860 4944 211
Size: 181 468 4084 507
Fragments arranged by size:
4084 507 468 181
BsaAI yAC'GTr
Cuts at: 2220 2593 2220
Size: 373 4867
BsaBI GATnn'nnATC
Cuts at: 6 2754 4791 6
Size: 2748 2037 455
Fragments arranged by size:
2748 2037 455
BsaHI Gr'CG_yC
Cuts at: 257 274 409 599 1185 1271 1322 2671 2919
Size: 17 135 190 586 86 51 1349 248
Cuts at: 2919 3621 5117 257
Size: 702 1496 380
Fragments arranged by size:
1496 1349 702 586 380 248 190 135
86 51 17
BsaWI w'CCGG_w
Cuts at: 486 945 2429 2950 3628 4039 4186 4460 486
Size: 459 1484 521 678 411 147 274 1266
Fragments arranged by size:
1484 1266 678 521 459 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 475 857 887 1681 1711 2179 2206 2209
Size: 30 382 30 794 30 468 27 3
Cuts at: 2209 2236 445
Size: 27 3449
Fragments arranged by size:
3449 794 468 382 30 30 30 27
27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 844 1486 1792 1819 1894 2167 166
Size: 678 642 306 27 75 273 3239
Fragments arranged by size:
3239 678 642 306 273 75 27
BseYI C'CCAG_C
Cuts at: 163 422 677 953 1151 1591 1608 2034 2230
Size: 259 255 276 198 440 17 426 196
Cuts at: 2230 2665 3423 4088 4496 163
Size: 435 758 665 408 907
Fragments arranged by size:
907 758 665 440 435 426 408 276
259 255 198 196 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 505 592 724 967 1141 138
Size: 367 87 132 243 174 4237
Fragments arranged by size:
4237 367 243 174 132 87
BsiEI CG_ry'CG
Cuts at: 104 2782 2828 4058 104
Size: 2678 46 1230 1286
Fragments arranged by size:
2678 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 404 1175 1546 1644 2219 2254 3032 3222 4082
Size: 771 371 98 575 35 778 190 860
Cuts at: 4082 404
Size: 1562
Fragments arranged by size:
1562 860 778 771 575 371 190 98
35
BsmI GAATG_Cn'
Cuts at: 2019 2019
Size: 5240
BsmBI CGTCTCn'nnnn_
Cuts at: 416 507 1515 416
Size: 91 1008 4141
Fragments arranged by size:
4141 1008 91
Bsp1286I G_dGCh'C
Cuts at: 340 404 677 935 945 1175 1214 1466 1488
Size: 64 273 258 10 230 39 252 22
Cuts at: 1488 1546 1618 1644 1914 2219 2254 2865 2958
Size: 58 72 26 270 305 35 611 93
Cuts at: 2958 3032 3222 3284 4082 340
Size: 74 190 62 798 1498
Fragments arranged by size:
1498 798 611 305 273 270 258 252
230 190 93 74 72 64 62 58
39 35 26 22 10
BspEI T'CCGG_A
Cuts at: 486 486
Size: 5240
BspHI T'CATG_A
Cuts at: 2120 3672 2120
Size: 1552 3688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 536 704 989 1080 1977 2290 2504 2806
Size: 339 168 285 91 897 313 214 302
Cuts at: 2806 3187 197
Size: 381 2250
Fragments arranged by size:
2250 897 381 339 313 302 285 214
168 91
BsrBI CCG'CTC
Cuts at: 50 575 764 1493 1797 3532 3586 5070 5235
Size: 525 189 729 304 1735 54 1484 165
Cuts at: 5235 50
Size: 55
Fragments arranged by size:
1735 1484 729 525 304 189 165 55
54
BsrDI GCAATG_nn'
Cuts at: 3152 3152
Size: 5240
BsrFI r'CCGG_y
Cuts at: 259 647 892 1181 3238 3419 259
Size: 388 245 289 2057 181 2080
Fragments arranged by size:
2080 2057 388 289 245 181
BssHII G'CGCG_C
Cuts at: 1281 1281
Size: 5240
BssSI C'ACGA_G
Cuts at: 3511 4219 3511
Size: 708 4532
BstAPI GCAn_nnn'nTGC
Cuts at: 528 2223 2617 528
Size: 1695 394 3151
Fragments arranged by size:
3151 1695 394
BstBI TT'CG_AA
Cuts at: 3601 3601
Size: 5240
BstEII G'GTnAC_C
Cuts at: 1953 1953
Size: 5240
BstXI CCAn_nnnn'nTGG
Cuts at: 924 2157 924
Size: 1233 4007
BstYI r'GATC_y
Cuts at: 1 569 992 2265 2732 2755 3090 3336 3642
Size: 568 423 1273 467 23 335 246 306
Cuts at: 3642 3654 3740 4792 1
Size: 12 86 1052 449
Fragments arranged by size:
1273 1052 568 467 449 423 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1469 1469
Size: 5240
BtgI C'CryG_G
Cuts at: 221 269 351 897 1367 1715 1931 221
Size: 48 82 546 470 348 216 3530
Fragments arranged by size:
3530 546 470 348 216 82 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 481 1131 1416 1547 2082 3190 3371 3534 481
Size: 650 285 131 535 1108 181 163 2187
Fragments arranged by size:
2187 1108 650 535 285 181 163 131
BtsI GCAGTG_nn'
Cuts at: 624 782 1425 1503 2483 624
Size: 158 643 78 980 3381
Fragments arranged by size:
3381 980 643 158 78
DraI TTT'AAA
Cuts at: 311 2239 311
Size: 1928 3312
DrdI GACnn_nn'nnGTC
Cuts at: 2946 4290 2946
Size: 1344 3896
EaeI y'GGCC_r
Cuts at: 348 728 797 894 1067 1868 2069 2825 2999
Size: 380 69 97 173 801 201 756 174
Cuts at: 2999 3390 3417 4762 348
Size: 391 27 1345 826
Fragments arranged by size:
1345 826 801 756 391 380 201 174
173 97 69 27
EagI C'GGCC_G
Cuts at: 2825 2825
Size: 5240
EarI CTCTTCn'nnn_
Cuts at: 106 771 1664 3263 3473 106
Size: 665 893 1599 210 1873
Fragments arranged by size:
1873 1599 893 665 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3448 4190 4336 5050 3448
Size: 742 146 714 3638
Fragments arranged by size:
3638 742 714 146
EcoICRI GAG'CTC
Cuts at: 2252 2252
Size: 5240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 379 691 1018 1189 1210 1279 1462 1497
Size: 143 312 327 171 21 69 183 35
Cuts at: 1497 1530 1726 1747 1822 1876 1984 2020 2233
Size: 33 196 21 75 54 108 36 213
Cuts at: 2233 3065 3497 3850 4512 236
Size: 832 432 353 662 964
Fragments arranged by size:
964 832 662 432 353 327 312 213
196 183 171 143 108 75 69 54
36 35 33 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 826 1348 1774 1858 1905 826
Size: 522 426 84 47 4161
Fragments arranged by size:
4161 522 426 84 47
EcoO109I rG'GnC_Cy
Cuts at: 286 708 1211 1446 1463 2379 286
Size: 422 503 235 17 916 3147
Fragments arranged by size:
3147 916 503 422 235 17
EcoRI G'AATT_C
Cuts at: 2244 2244
Size: 5240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3259 3259
Size: 5240
FspI TGC'GCA
Cuts at: 703 2137 3021 703
Size: 1434 884 2922
Fragments arranged by size:
2922 1434 884
HaeII r_GCGC'y
Cuts at: 266 277 483 602 1188 1274 1553 1559 1609
Size: 11 206 119 586 86 279 6 50
Cuts at: 1609 1785 2032 2065 2674 2922 4152 4923 266
Size: 176 247 33 609 248 1230 771 583
Fragments arranged by size:
1230 771 609 586 583 279 248 247
206 176 119 86 50 33 11 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 1528 1560 1681 1713 3082 3112 3114
Size: 32 1051 32 121 32 1369 30 2
Cuts at: 3114 3144 445
Size: 30 2541
Fragments arranged by size:
2541 1369 1051 121 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2279 2279
Size: 5240
KasI G'GCGC_C
Cuts at: 273 598 1184 1270 2670 2918 273
Size: 325 586 86 1400 248 2595
Fragments arranged by size:
2595 1400 586 325 248 86
KpnI G_GTAC'C
Cuts at: 2260 2260
Size: 5240
MluI A'CGCG_T
Cuts at: 4673 4673
Size: 5240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 476 3999 4183 476
Size: 3523 184 1533
Fragments arranged by size:
3523 1533 184
MscI TGG'CCA
Cuts at: 350 1069 1870 3001 350
Size: 719 801 1131 2589
Fragments arranged by size:
2589 1131 801 719
MslI CAynn'nnrTG
Cuts at: 1366 1756 2050 2071 3229 3356 1366
Size: 390 294 21 1158 127 3250
Fragments arranged by size:
3250 1158 390 294 127 21
MspA1I CmG'CkG
Cuts at: 271 327 635 957 1155 1295 1737 1998 2351
Size: 56 308 322 198 140 442 261 353
Cuts at: 2351 2665 3025 3809 4054 271
Size: 314 360 784 245 1457
Fragments arranged by size:
1457 784 442 360 353 322 314 308
261 245 198 140 56
NaeI GCC'GGC
Cuts at: 261 649 894 1183 3421 261
Size: 388 245 289 2238 2080
Fragments arranged by size:
2238 2080 388 289 245
NarI GG'CG_CC
Cuts at: 274 599 1185 1271 2671 2919 274
Size: 325 586 86 1400 248 2595
Fragments arranged by size:
2595 1400 586 325 248 86
NcoI C'CATG_G
Cuts at: 897 1715 897
Size: 818 4422
NgoMIV G'CCGG_C
Cuts at: 259 647 892 1181 3419 259
Size: 388 245 289 2238 2080
Fragments arranged by size:
2238 2080 388 289 245
NruI TCG'CGA
Cuts at: 1037 1037
Size: 5240
NsiI A_TGCA'T
Cuts at: 497 497
Size: 5240
NspI r_CATG'y
Cuts at: 1248 2293 2793 4396 1248
Size: 1045 500 1603 2092
Fragments arranged by size:
2092 1603 1045 500
PciI A'CATG_T
Cuts at: 4392 4392
Size: 5240
PfoI T'CCnGG_A
Cuts at: 1644 1922 2210 1644
Size: 278 288 4674
Fragments arranged by size:
4674 288 278
PmeI GTTT'AAAC
Cuts at: 2239 2239
Size: 5240
PmlI CAC'GTG
Cuts at: 2220 2220
Size: 5240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1198 1230 3686 3718 1198
Size: 32 2456 32 2720
Fragments arranged by size:
2720 2456 32 32
PpuMI rG'GwC_Cy
Cuts at: 286 1446 286
Size: 1160 4080
PshAI GACnn'nnGTC
Cuts at: 1951 1951
Size: 5240
PspOMI G'GGCC_C
Cuts at: 1210 1462 1210
Size: 252 4988
PstI C_TGCA'G
Cuts at: 137 566 1742 1775 1973 2287 2972 137
Size: 429 1176 33 198 314 685 2405
Fragments arranged by size:
2405 1176 685 429 314 198 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5240
PvuII CAG'CTG
Cuts at: 327 1155 1737 1998 2665 3025 327
Size: 828 582 261 667 360 2542
Fragments arranged by size:
2542 828 667 582 360 261
RsrII CG'GwC_CG
Cuts at: 3435 3435
Size: 5240
SacI G_AGCT'C
Cuts at: 2254 2254
Size: 5240
SacII CC_GC'GG
Cuts at: 272 272
Size: 5240
SalI G'TCGA_C
Cuts at: 2277 2277
Size: 5240
SapI GCTCTTCn'nnn_
Cuts at: 771 1664 3263 3473 771
Size: 893 1599 210 2538
Fragments arranged by size:
2538 1599 893 210
SbfI CC_TGCA'GG
Cuts at: 1775 2287 1775
Size: 512 4728
ScaI AGT'ACT
Cuts at: 4757 4757
Size: 5240
SexAI A'CCwGG_T
Cuts at: 869 869
Size: 5240
SfcI C'TryA_G
Cuts at: 33 133 562 1738 1771 1785 1969 2283 2968
Size: 100 429 1176 33 14 184 314 685
Cuts at: 2968 3936 4127 33
Size: 968 191 1146
Fragments arranged by size:
1176 1146 968 685 429 314 191 184
100 33 14
SfoI GGC'GCC
Cuts at: 275 600 1186 1272 2672 2920 275
Size: 325 586 86 1400 248 2595
Fragments arranged by size:
2595 1400 586 325 248 86
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5240
SgrAI Cr'CCGG_yG
Cuts at: 259 1181 259
Size: 922 4318
SmaI CCC'GGG
Cuts at: 1384 1483 1951 2262 1384
Size: 99 468 311 4362
Fragments arranged by size:
4362 468 311 99
SmlI C'TyrA_G
Cuts at: 2395 3310 4024 4286 2395
Size: 915 714 262 3349
Fragments arranged by size:
3349 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5240
SphI G_CATG'C
Cuts at: 2293 2793 2293
Size: 500 4740
SrfI GCCC'GGGC
Cuts at: 1483 1483
Size: 5240
StyI C'CwwG_G
Cuts at: 897 962 1226 1715 2374 2728 897
Size: 65 264 489 659 354 3409
Fragments arranged by size:
3409 659 489 354 264 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 374 1372 2823 2956 3415 3624 374
Size: 998 1451 133 459 209 1990
Fragments arranged by size:
1990 1451 998 459 209 133
TatI w'GTAC_w
Cuts at: 4755 4755
Size: 5240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 857 889 1904 1936 2179 2211 4757 4789 857
Size: 32 1015 32 243 32 2546 32 1308
Fragments arranged by size:
2546 1308 1015 243 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 853 3037 853
Size: 2184 3056
XbaI T'CTAG_A
Cuts at: 2271 2271
Size: 5240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2735 2735
Size: 5240
XmaI C'CCGG_G
Cuts at: 1382 1481 1949 2260 1382
Size: 99 468 311 4362
Fragments arranged by size:
4362 468 311 99
ZraI GAC'GTC
Cuts at: 410 410
Size: 5240
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI
NspI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AlfI AloI AscI AvrII BaeI
BmtI BsiWI BsrGI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
PacI PasI PflMI PsiI PspXI PsrI SanDI SfiI
SnaBI SspI StuI SwaI XhoI XmnI