Restriction map for
pF1KA0474
(Circular) (Six-base) MAPSORT of: pF1KA0474.seq Check: 8639 from: 1 to: 5156
pF1KA0474 5156 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 494 494
Size: 5156
AatII G_ACGT'C
Cuts at: 599 599
Size: 5156
AccI GT'mk_AC
Cuts at: 2194 2194
Size: 5156
Acc65I G'GTAC_C
Cuts at: 2172 2172
Size: 5156
AclI AA'CG_TT
Cuts at: 4996 4996
Size: 5156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 2981 3413 3766 4428 723
Size: 2258 432 353 662 1451
Fragments arranged by size:
2258 1451 662 432 353
AflIII A'CryG_T
Cuts at: 908 2508 4308 4589 908
Size: 1600 1800 281 1475
Fragments arranged by size:
1800 1600 1475 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1298 1330 1298
Size: 32 5124
AlwNI CAG_nnn'CTG
Cuts at: 2461 3899 2461
Size: 1438 3718
ApaI G_GGCC'C
Cuts at: 1087 1660 1087
Size: 573 4583
ApaLI G'TGCA_C
Cuts at: 205 3994 205
Size: 3789 1367
ApoI r'AATT_y
Cuts at: 781 1195 2160 4509 4765 781
Size: 414 965 2349 256 1172
Fragments arranged by size:
2349 1172 965 414 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5156
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5156
AvaI C'yCGr_G
Cuts at: 198 307 349 2095 2176 198
Size: 109 42 1746 81 3178
Fragments arranged by size:
3178 1746 109 81 42
BamHI G'GATC_C
Cuts at: 2181 2181
Size: 5156
BanI G'GyrC_C
Cuts at: 280 2172 2586 2834 2869 280
Size: 1892 414 248 35 2567
Fragments arranged by size:
2567 1892 414 248 35
BanII G_rGCy'C
Cuts at: 1087 1639 1660 1732 1781 1810 2170 3200 1087
Size: 552 21 72 49 29 360 1030 3043
Fragments arranged by size:
3043 1030 552 360 72 49 29 21
BbeI G_GCGC'C
Cuts at: 2590 2838 2590
Size: 248 4908
BbsI GAAGACnn'nnnn_
Cuts at: 438 599 987 1753 438
Size: 161 388 766 3841
Fragments arranged by size:
3841 766 388 161
BbvCI CC'TCA_GC
Cuts at: 1073 1073
Size: 5156
BciVI GTATCCnnnnn_n'
Cuts at: 3054 4110 5156 3054
Size: 1056 1046 3054
Fragments arranged by size:
3054 1056 1046
BclI T'GATC_A
Cuts at: 1214 2676 1214
Size: 1462 3694
BglI GCCn_nnn'nGGC
Cuts at: 1091 1525 1664 1091
Size: 434 139 4583
Fragments arranged by size:
4583 434 139
BglII A'GATC_T
Cuts at: 2671 4708 2671
Size: 2037 3119
BlpI GC'TnA_GC
Cuts at: 1447 2268 1447
Size: 821 4335
Bme1580I G_kGCm'C
Cuts at: 209 283 1025 1087 1136 1660 1766 2781 2874
Size: 74 742 62 49 524 106 1015 93
Cuts at: 2874 3998 209
Size: 1124 1367
Fragments arranged by size:
1367 1124 1015 742 524 106 93 74
62 49
BmgBI CAC'GTC
Cuts at: 840 1767 840
Size: 927 4229
BmrI ACTGGGnnnn_n'
Cuts at: 249 2368 2449 2784 4928 249
Size: 2119 81 335 2144 477
Fragments arranged by size:
2144 2119 477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3523 3523
Size: 5156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 1309 1981 2044 1282
Size: 27 672 63 4394
Fragments arranged by size:
4394 672 63 27
Bpu10I CC'TnA_GC
Cuts at: 499 1073 499
Size: 574 4582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1855 2332 3211 3925 4223 439
Size: 1416 477 879 714 298 1372
Fragments arranged by size:
1416 1372 879 714 477 298
BsaI GGTCTCn'nnnn_
Cuts at: 1113 1837 4860 1113
Size: 724 3023 1409
Fragments arranged by size:
3023 1409 724
BsaAI yAC'GTr
Cuts at: 909 2018 2509 909
Size: 1109 491 3556
Fragments arranged by size:
3556 1109 491
BsaBI GATnn'nnATC
Cuts at: 6 2104 2670 4707 6
Size: 2098 566 2037 455
Fragments arranged by size:
2098 2037 566 455
BsaHI Gr'CG_yC
Cuts at: 596 695 941 1575 2066 2587 2835 3537 5033
Size: 99 246 634 491 521 248 702 1496
Cuts at: 5033 596
Size: 719
Fragments arranged by size:
1496 719 702 634 521 491 248 246
99
BsaWI w'CCGG_w
Cuts at: 377 1412 2345 2866 3544 3955 4102 4376 377
Size: 1035 933 521 678 411 147 274 1157
Fragments arranged by size:
1157 1035 933 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 886 916 1136 1166 1768 1798 1954 1984 886
Size: 30 220 30 602 30 156 30 4058
Fragments arranged by size:
4058 602 220 156 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 842 1279 1878 1939 193
Size: 649 437 599 61 3410
Fragments arranged by size:
3410 649 599 437 61
BseYI C'CCAG_C
Cuts at: 944 2138 2142 2581 3339 4004 4412 944
Size: 1194 4 439 758 665 408 1688
Fragments arranged by size:
1688 1194 758 665 439 408 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1732 1087
Size: 645 4511
BsiEI CG_ry'CG
Cuts at: 104 2698 2744 3974 104
Size: 2594 46 1230 1286
Fragments arranged by size:
2594 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 209 485 626 1810 2170 2948 3138 3998 209
Size: 276 141 1184 360 778 190 860 1367
Fragments arranged by size:
1367 1184 860 778 360 276 190 141
BsmBI CGTCTCn'nnnn_
Cuts at: 1285 1773 1921 1285
Size: 488 148 4520
Fragments arranged by size:
4520 488 148
Bsp1286I G_dGCh'C
Cuts at: 209 283 485 626 1025 1087 1136 1639 1660
Size: 74 202 141 399 62 49 503 21
Cuts at: 1660 1732 1766 1781 1810 2170 2781 2874 2948
Size: 72 34 15 29 360 611 93 74
Cuts at: 2948 3138 3200 3998 209
Size: 190 62 798 1367
Fragments arranged by size:
1367 798 611 503 399 360 202 190
141 93 74 74 72 62 62 49
34 29 21 15
BspEI T'CCGG_A
Cuts at: 1412 1412
Size: 5156
BspHI T'CATG_A
Cuts at: 3588 3588
Size: 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 2206 2420 2722 3103 494
Size: 1712 214 302 381 2547
Fragments arranged by size:
2547 1712 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1269 1840 1886 3448 3502 4986 5151 50
Size: 1219 571 46 1562 54 1484 165 55
Fragments arranged by size:
1562 1484 1219 571 165 55 54 46
BsrDI GCAATG_nn'
Cuts at: 1537 3068 1537
Size: 1531 3625
BsrFI r'CCGG_y
Cuts at: 2068 3154 3335 2068
Size: 1086 181 3889
Fragments arranged by size:
3889 1086 181
BssSI C'ACGA_G
Cuts at: 208 1641 3427 4135 208
Size: 1433 1786 708 1229
Fragments arranged by size:
1786 1433 1229 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2133 2533 2133
Size: 400 4756
BstBI TT'CG_AA
Cuts at: 3517 3517
Size: 5156
BstXI CCAn_nnnn'nTGG
Cuts at: 1920 1920
Size: 5156
BstYI r'GATC_y
Cuts at: 1 2105 2181 2648 2671 3006 3252 3558 3570
Size: 2104 76 467 23 335 246 306 12
Cuts at: 3570 3656 4708 1
Size: 86 1052 449
Fragments arranged by size:
2104 1052 467 449 335 306 246 86
76 23 12
Bsu36I CC'TnA_GG
Cuts at: 1977 1977
Size: 5156
BtgI C'CryG_G
Cuts at: 846 1430 1439 1604 846
Size: 584 9 165 4398
Fragments arranged by size:
4398 584 165 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3106 3287 3450 3106
Size: 181 163 4812
Fragments arranged by size:
4812 181 163
BtsI GCAGTG_nn'
Cuts at: 319 1115 1377 1590 2399 319
Size: 796 262 213 809 3076
Fragments arranged by size:
3076 809 796 262 213
DraI TTT'AAA
Cuts at: 2155 2155
Size: 5156
DrdI GACnn_nn'nnGTC
Cuts at: 667 2862 4206 667
Size: 2195 1344 1617
Fragments arranged by size:
2195 1617 1344
EaeI y'GGCC_r
Cuts at: 435 789 1517 2741 2915 3306 3333 4678 435
Size: 354 728 1224 174 391 27 1345 913
Fragments arranged by size:
1345 1224 913 728 391 354 174 27
EagI C'GGCC_G
Cuts at: 2741 2741
Size: 5156
EarI CTCTTCn'nnn_
Cuts at: 742 1604 1627 1714 3179 3389 742
Size: 862 23 87 1465 210 2509
Fragments arranged by size:
2509 1465 862 210 87 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3364 4106 4252 4966 3364
Size: 742 146 714 3554
Fragments arranged by size:
3554 742 714 146
EcoICRI GAG'CTC
Cuts at: 1808 2168 1808
Size: 360 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 1282 1309 1981 2044 2981 3413 3766 4428
Size: 559 27 672 63 937 432 353 662
Cuts at: 4428 723
Size: 1451
Fragments arranged by size:
1451 937 672 662 559 432 353 63
27
EcoNI CCTnn'n_nnAGG
Cuts at: 1102 1519 1102
Size: 417 4739
EcoO109I rG'GnC_Cy
Cuts at: 229 1083 1084 1180 2082 2295 229
Size: 854 1 96 902 213 3090
Fragments arranged by size:
3090 902 854 213 96 1
EcoRI G'AATT_C
Cuts at: 2160 2160
Size: 5156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 779 3175 779
Size: 2396 2760
FspI TGC'GCA
Cuts at: 2937 2937
Size: 5156
HaeII r_GCGC'y
Cuts at: 1685 1870 2590 2838 4068 4839 1685
Size: 185 720 248 1230 771 2002
Fragments arranged by size:
2002 1230 771 720 248 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1136 1168 1768 1780 1800 1812 1954 1986 2998
Size: 32 600 12 20 12 142 32 1012
Cuts at: 2998 3028 3030 3060 1136
Size: 30 2 30 3232
Fragments arranged by size:
3232 1012 600 142 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 2195 2195
Size: 5156
HindIII A'AGCT_T
Cuts at: 709 709
Size: 5156
KasI G'GCGC_C
Cuts at: 2586 2834 2586
Size: 248 4908
KpnI G_GTAC'C
Cuts at: 2176 2176
Size: 5156
MluI A'CGCG_T
Cuts at: 4589 4589
Size: 5156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1064 1766 3915 4099 1064
Size: 702 2149 184 2121
Fragments arranged by size:
2149 2121 702 184
MscI TGG'CCA
Cuts at: 791 1519 2917 791
Size: 728 1398 3030
Fragments arranged by size:
3030 1398 728
MslI CAynn'nnrTG
Cuts at: 3145 3272 3145
Size: 127 5029
MspA1I CmG'CkG
Cuts at: 957 1165 1487 1760 2112 2142 2267 2581 2941
Size: 208 322 273 352 30 125 314 360
Cuts at: 2941 3725 3970 957
Size: 784 245 2143
Fragments arranged by size:
2143 784 360 352 322 314 273 245
208 125 30
NaeI GCC'GGC
Cuts at: 2070 3337 2070
Size: 1267 3889
NarI GG'CG_CC
Cuts at: 2587 2835 2587
Size: 248 4908
NcoI C'CATG_G
Cuts at: 1430 1439 1430
Size: 9 5147
NgoMIV G'CCGG_C
Cuts at: 2068 3335 2068
Size: 1267 3889
NspI r_CATG'y
Cuts at: 670 1054 2136 2209 2709 4312 670
Size: 384 1082 73 500 1603 1514
Fragments arranged by size:
1603 1514 1082 500 384 73
PasI CC'CwG_GG
Cuts at: 1627 1627
Size: 5156
PciI A'CATG_T
Cuts at: 4308 4308
Size: 5156
PflMI CCAn_nnn'nTGG
Cuts at: 1431 1431
Size: 5156
PfoI T'CCnGG_A
Cuts at: 998 1154 1190 1286 998
Size: 156 36 96 4868
Fragments arranged by size:
4868 156 96 36
PmeI GTTT'AAAC
Cuts at: 2155 2155
Size: 5156
PmlI CAC'GTG
Cuts at: 909 2018 909
Size: 1109 4047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 5124
PpuMI rG'GwC_Cy
Cuts at: 229 2082 229
Size: 1853 3303
PspOMI G'GGCC_C
Cuts at: 1083 1656 1083
Size: 573 4583
PspXI vC'TCGA_Gb
Cuts at: 349 349
Size: 5156
PstI C_TGCA'G
Cuts at: 326 809 2203 2888 326
Size: 483 1394 685 2594
Fragments arranged by size:
2594 1394 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5156
PvuII CAG'CTG
Cuts at: 2112 2142 2581 2941 2112
Size: 30 439 360 4327
Fragments arranged by size:
4327 439 360 30
RsrII CG'GwC_CG
Cuts at: 3351 3351
Size: 5156
SacI G_AGCT'C
Cuts at: 1810 2170 1810
Size: 360 4796
SalI G'TCGA_C
Cuts at: 2193 2193
Size: 5156
SanDI GG'GwC_CC
Cuts at: 2082 2082
Size: 5156
SapI GCTCTTCn'nnn_
Cuts at: 1627 3179 3389 1627
Size: 1552 210 3394
Fragments arranged by size:
3394 1552 210
SbfI CC_TGCA'GG
Cuts at: 2203 2203
Size: 5156
ScaI AGT'ACT
Cuts at: 4673 4673
Size: 5156
SfcI C'TryA_G
Cuts at: 33 322 805 2010 2199 2884 3852 4043 33
Size: 289 483 1205 189 685 968 191 1146
Fragments arranged by size:
1205 1146 968 685 483 289 191 189
SfiI GGCCn_nnn'nGGCC
Cuts at: 1091 1091
Size: 5156
SfoI GGC'GCC
Cuts at: 2588 2836 2588
Size: 248 4908
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5156
SmaI CCC'GGG
Cuts at: 2178 2178
Size: 5156
SmlI C'TyrA_G
Cuts at: 349 454 1870 2311 3226 3940 4202 349
Size: 105 1416 441 915 714 262 1303
Fragments arranged by size:
1416 1303 915 714 441 262 105
SpeI A'CTAG_T
Cuts at: 67 2044 67
Size: 1977 3179
SphI G_CATG'C
Cuts at: 1054 2209 2709 1054
Size: 1155 500 3501
Fragments arranged by size:
3501 1155 500
StuI AGG'CCT
Cuts at: 833 1958 833
Size: 1125 4031
StyI C'CwwG_G
Cuts at: 338 632 1430 1439 1520 2290 2644 338
Size: 294 798 9 81 770 354 2850
Fragments arranged by size:
2850 798 770 354 294 81 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 877 2739 2872 3331 3540 877
Size: 1862 133 459 209 2493
Fragments arranged by size:
2493 1862 459 209 133
TatI w'GTAC_w
Cuts at: 872 4671 872
Size: 3799 1357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1309 1341 1607 1639 2074 2106 4673 4705 1309
Size: 32 266 32 435 32 2567 32 1760
Fragments arranged by size:
2567 1760 435 266 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2032 2953 2032
Size: 921 4235
XbaI T'CTAG_A
Cuts at: 2187 2187
Size: 5156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1335 1437 2651 1335
Size: 102 1214 3840
Fragments arranged by size:
3840 1214 102
XhoI C'TCGA_G
Cuts at: 349 349
Size: 5156
XmaI C'CCGG_G
Cuts at: 2176 2176
Size: 5156
ZraI GAC'GTC
Cuts at: 597 597
Size: 5156
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI SwaI XmnI