Restriction map for
pF1KA0461
(Circular) (Six-base) MAPSORT of: pF1KA0461.seq Check: 4742 from: 1 to: 7184
pF1KA0461 7184 bp
With 182 enzymes: SgfI *
September 25, 2009 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 837 2296 837
Size: 1459 5725
AccI GT'mk_AC
Cuts at: 4222 4222
Size: 7184
Acc65I G'GTAC_C
Cuts at: 523 4200 523
Size: 3677 3507
AclI AA'CG_TT
Cuts at: 7024 7024
Size: 7184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 1567 1843 2660 3826 4039 4094 5009 5441
Size: 437 276 817 1166 213 55 915 432
Cuts at: 5441 6188 6456 1130
Size: 747 268 1858
Fragments arranged by size:
1858 1166 915 817 747 437 432 276
268 213 55
AfeI AGC'GCT
Cuts at: 3910 3910
Size: 7184
AflIII A'CryG_T
Cuts at: 1127 4536 5640 6617 1127
Size: 3409 1104 977 1694
Fragments arranged by size:
3409 1694 1104 977
AgeI A'CCGG_T
Cuts at: 5572 5572
Size: 7184
AleI CACnn'nnGTG
Cuts at: 2008 2008
Size: 7184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2500 3637 2500
Size: 1137 6047
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1181 1213 3082 3114 1181
Size: 32 1869 32 5251
Fragments arranged by size:
5251 1869 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1240 3817 4489 6056 1240
Size: 2577 672 1567 2368
Fragments arranged by size:
2577 2368 1567 672
ApaLI G'TGCA_C
Cuts at: 5954 5954
Size: 7184
ApoI r'AATT_y
Cuts at: 1404 2370 3728 4188 6537 6793 1404
Size: 966 1358 460 2349 256 1795
Fragments arranged by size:
2349 1795 1358 966 460 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7184
AvaI C'yCGr_G
Cuts at: 2027 2297 3578 3800 4204 2027
Size: 270 1281 222 404 5007
Fragments arranged by size:
5007 1281 404 270 222
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1280 1313 3338 3371 1280
Size: 33 2025 33 5093
Fragments arranged by size:
5093 2025 33 33
BamHI G'GATC_C
Cuts at: 4209 4209
Size: 7184
BanI G'GyrC_C
Cuts at: 523 1868 2002 3182 4200 4614 4862 4897 523
Size: 1345 134 1180 1018 414 248 35 2810
Fragments arranged by size:
2810 1345 1180 1018 414 248 134 35
BanII G_rGCy'C
Cuts at: 2347 2797 4198 5228 2347
Size: 450 1401 1030 4303
Fragments arranged by size:
4303 1401 1030 450
BbeI G_GCGC'C
Cuts at: 4618 4866 4618
Size: 248 6936
BbsI GAAGACnn'nnnn_
Cuts at: 1007 1821 2121 4037 4090 1007
Size: 814 300 1916 53 4101
Fragments arranged by size:
4101 1916 814 300 53
BciVI GTATCCnnnnn_n'
Cuts at: 1683 1785 2064 3339 5082 5843 7184 1683
Size: 102 279 1275 1743 761 1341 1683
Fragments arranged by size:
1743 1683 1341 1275 761 279 102
BclI T'GATC_A
Cuts at: 2318 2778 4704 2318
Size: 460 1926 4798
Fragments arranged by size:
4798 1926 460
BfrBI ATG'CAT
Cuts at: 1846 3749 1846
Size: 1903 5281
BglI GCCn_nnn'nGGC
Cuts at: 609 3439 3947 609
Size: 2830 508 3846
Fragments arranged by size:
3846 2830 508
BglII A'GATC_T
Cuts at: 1637 3146 3329 4164 4699 6736 1637
Size: 1509 183 835 535 2037 2085
Fragments arranged by size:
2085 2037 1509 835 535 183
BlpI GC'TnA_GC
Cuts at: 2633 3358 4296 2633
Size: 725 938 5521
Fragments arranged by size:
5521 938 725
Bme1580I G_kGCm'C
Cuts at: 304 3403 4809 4902 5958 304
Size: 3099 1406 93 1056 1530
Fragments arranged by size:
3099 1530 1406 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 522 675 757 1036 1187 2791 4396 4477 4812
Size: 153 82 279 151 1604 1605 81 335
Cuts at: 4812 6956 522
Size: 2144 750
Fragments arranged by size:
2144 1605 1604 750 335 279 153 151
82 81
BmtI G_CTAG'C
Cuts at: 808 2821 808
Size: 2013 5171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5551 5551
Size: 7184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 1039 1660 1724 2005 2230 755
Size: 284 621 64 281 225 5709
Fragments arranged by size:
5709 621 284 281 225 64
Bpu10I CC'TnA_GC
Cuts at: 2658 2813 2658
Size: 155 7029
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2499 4360 5239 5731 6029 2499
Size: 1861 879 492 298 3654
Fragments arranged by size:
3654 1861 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3085 4120 6888 3085
Size: 1035 2768 3381
Fragments arranged by size:
3381 2768 1035
BsaAI yAC'GTr
Cuts at: 1497 2259 4537 1497
Size: 762 2278 4144
Fragments arranged by size:
4144 2278 762
BsaBI GATnn'nnATC
Cuts at: 6 4698 6735 6
Size: 4692 2037 455
Fragments arranged by size:
4692 2037 455
BsaHI Gr'CG_yC
Cuts at: 4615 4863 5565 7061 4615
Size: 248 702 1496 4738
Fragments arranged by size:
4738 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2549 4373 4894 5572 5846 5993 6404 2549
Size: 1824 521 678 274 147 411 3329
Fragments arranged by size:
3329 1824 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 133 163 1658 1688 2851 2881 3082 3112 3556
Size: 30 1495 30 1163 30 201 30 444
Cuts at: 3556 3586 133
Size: 30 3731
Fragments arranged by size:
3731 1495 1163 444 201 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 157 160 1734 2097 2564 3103 4003 4024
Size: 3 3 1574 363 467 539 900 21
Cuts at: 4024 154
Size: 3314
Fragments arranged by size:
3314 1574 900 539 467 363 21 3
3
BseYI C'CCAG_C
Cuts at: 686 706 1394 2074 2628 2664 2945 3059 3708
Size: 20 688 680 554 36 281 114 649
Cuts at: 3708 3861 4609 5367 5944 6440 686
Size: 153 748 758 577 496 1430
Fragments arranged by size:
1430 758 748 688 680 649 577 554
496 281 153 114 36 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2235 2382 2235
Size: 147 7037
BsiEI CG_ry'CG
Cuts at: 104 4726 4772 5980 104
Size: 4622 46 1208 1308
Fragments arranged by size:
4622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4198 4976 5166 5958 4198
Size: 778 190 792 5424
Fragments arranged by size:
5424 792 778 190
BsmI GAATG_Cn'
Cuts at: 354 1846 3252 3387 354
Size: 1492 1406 135 4151
Fragments arranged by size:
4151 1492 1406 135
Bsp1286I G_dGCh'C
Cuts at: 304 2347 2797 3403 4198 4809 4902 4976 5166
Size: 2043 450 606 795 611 93 74 190
Cuts at: 5166 5228 5958 304
Size: 62 730 1530
Fragments arranged by size:
2043 1530 795 730 611 606 450 190
93 74 62
BspEI T'CCGG_A
Cuts at: 6404 6404
Size: 7184
BspHI T'CATG_A
Cuts at: 1475 6360 1475
Size: 4885 2299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 837 2296 4234 4448 4750 5131 837
Size: 1459 1938 214 302 381 2890
Fragments arranged by size:
2890 1938 1459 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5476 5530 7014 7179 50
Size: 5426 54 1484 165 55
Fragments arranged by size:
5426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 452 839 897 1848 5096 452
Size: 387 58 951 3248 2540
Fragments arranged by size:
3248 2540 951 387 58
BsrFI r'CCGG_y
Cuts at: 1904 2195 5182 5363 5572 1904
Size: 291 2987 181 209 3516
Fragments arranged by size:
3516 2987 291 209 181
BsrGI T'GTAC_A
Cuts at: 435 3270 435
Size: 2835 4349
BssSI C'ACGA_G
Cuts at: 2531 5455 5813 2531
Size: 2924 358 3902
Fragments arranged by size:
3902 2924 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1901 3247 3817 4561 1901
Size: 1346 570 744 4524
Fragments arranged by size:
4524 1346 744 570
BstBI TT'CG_AA
Cuts at: 660 5545 660
Size: 4885 2299
BstXI CCAn_nnnn'nTGG
Cuts at: 256 949 1063 1983 2355 2448 256
Size: 693 114 920 372 93 4992
Fragments arranged by size:
4992 920 693 372 114 93
BstYI r'GATC_y
Cuts at: 1 1637 2225 2470 3146 3329 4164 4209 4676
Size: 1636 588 245 676 183 835 45 467
Cuts at: 4676 4699 5034 5280 6292 6378 6390 6736 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1636 1012 835 676 588 467 449 346
335 246 245 183 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 1412 3230 1412
Size: 1818 5366
BtgI C'CryG_G
Cuts at: 157 2335 2488 157
Size: 2178 153 4853
Fragments arranged by size:
4853 2178 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1337 2444 3625 5134 5315 5478 1337
Size: 1107 1181 1509 181 163 3043
Fragments arranged by size:
3043 1509 1181 1107 181 163
BtsI GCAGTG_nn'
Cuts at: 725 839 1890 2262 3369 3707 4427 725
Size: 114 1051 372 1107 338 720 3482
Fragments arranged by size:
3482 1107 1051 720 372 338 114
DraI TTT'AAA
Cuts at: 2367 4183 2367
Size: 1816 5368
DrdI GACnn_nn'nnGTC
Cuts at: 4890 5748 4890
Size: 858 6326
EaeI y'GGCC_r
Cuts at: 644 899 2438 2523 4769 4943 5334 5361 6706
Size: 255 1539 85 2246 174 391 27 1345
Cuts at: 6706 644
Size: 1122
Fragments arranged by size:
2246 1539 1345 1122 391 255 174 85
27
EagI C'GGCC_G
Cuts at: 4769 4769
Size: 7184
EarI CTCTTCn'nnn_
Cuts at: 1038 1263 1870 2158 2335 2636 3026 3262 3653
Size: 225 607 288 177 301 390 236 391
Cuts at: 3653 4070 5207 5417 1038
Size: 417 1137 210 2805
Fragments arranged by size:
2805 1137 607 417 391 390 301 288
236 225 210 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5392 5702 5848 6994 5392
Size: 310 146 1146 5582
Fragments arranged by size:
5582 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4196 4196
Size: 7184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 755 1039 1130 1567 1660 1724 1843 2005 2230
Size: 284 91 437 93 64 119 162 225
Cuts at: 2230 2660 3826 4039 4094 5009 5441 6188 6456
Size: 430 1166 213 55 915 432 747 268
Cuts at: 6456 755
Size: 1483
Fragments arranged by size:
1483 1166 915 747 437 432 430 284
268 225 213 162 119 93 91 64
55
EcoNI CCTnn'n_nnAGG
Cuts at: 3229 3229
Size: 7184
EcoO109I rG'GnC_Cy
Cuts at: 4323 4323
Size: 7184
EcoRI G'AATT_C
Cuts at: 4188 4188
Size: 7184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3640 5203 3640
Size: 1563 5621
FspI TGC'GCA
Cuts at: 4965 4965
Size: 7184
HaeII r_GCGC'y
Cuts at: 2081 3912 4618 4866 5888 6867 2081
Size: 1831 706 248 1022 979 2398
Fragments arranged by size:
2398 1831 1022 979 706 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 267 283 299 780 812 2310 2342 2806
Size: 16 16 16 481 32 1498 32 464
Cuts at: 2806 2838 2851 2883 3316 3348 3556 3588 3621
Size: 32 13 32 433 32 208 32 33
Cuts at: 3621 3653 4117 4149 5026 5056 5058 5088 251
Size: 32 464 32 877 30 2 30 2347
Fragments arranged by size:
2347 1498 877 481 464 464 433 208
33 32 32 32 32 32 32 32
32 30 30 16 16 16 13 2
HincII GTy'rAC
Cuts at: 3575 4223 3575
Size: 648 6536
HindIII A'AGCT_T
Cuts at: 1112 1945 2878 1112
Size: 833 933 5418
Fragments arranged by size:
5418 933 833
KasI G'GCGC_C
Cuts at: 4614 4862 4614
Size: 248 6936
KpnI G_GTAC'C
Cuts at: 527 4204 527
Size: 3677 3507
MfeI C'AATT_G
Cuts at: 4082 4082
Size: 7184
MluI A'CGCG_T
Cuts at: 6617 6617
Size: 7184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 131 528 992 1875 5855 6039 131
Size: 397 464 883 3980 184 1276
Fragments arranged by size:
3980 1276 883 464 397 184
MscI TGG'CCA
Cuts at: 901 2440 2525 4945 901
Size: 1539 85 2420 3140
Fragments arranged by size:
3140 2420 1539 85
MslI CAynn'nnrTG
Cuts at: 2008 3625 5173 5300 2008
Size: 1617 1548 127 3892
Fragments arranged by size:
3892 1617 1548 127
MspA1I CmG'CkG
Cuts at: 232 1004 1983 2100 2632 2742 2865 2926 3846
Size: 772 979 117 532 110 123 61 920
Cuts at: 3846 4295 4609 4969 5982 6227 232
Size: 449 314 360 1013 245 1189
Fragments arranged by size:
1189 1013 979 920 772 532 449 360
314 245 123 117 110 61
NaeI GCC'GGC
Cuts at: 1906 2197 5365 1906
Size: 291 3168 3725
Fragments arranged by size:
3725 3168 291
NarI GG'CG_CC
Cuts at: 4615 4863 4615
Size: 248 6936
NcoI C'CATG_G
Cuts at: 157 157
Size: 7184
NdeI CA'TA_TG
Cuts at: 1484 1682 1484
Size: 198 6986
NgoMIV G'CCGG_C
Cuts at: 1904 2195 5363 1904
Size: 291 3168 3725
Fragments arranged by size:
3725 3168 291
NheI G'CTAG_C
Cuts at: 804 2817 804
Size: 2013 5171
NsiI A_TGCA'T
Cuts at: 1848 3751 1848
Size: 1903 5281
NspI r_CATG'y
Cuts at: 1131 2207 3507 3624 3824 3984 4237 4737 5644
Size: 1076 1300 117 200 160 253 500 907
Cuts at: 5644 1131
Size: 2671
Fragments arranged by size:
2671 1300 1076 907 500 253 200 160
117
PasI CC'CwG_GG
Cuts at: 936 2988 936
Size: 2052 5132
PciI A'CATG_T
Cuts at: 1127 5640 1127
Size: 4513 2671
PfoI T'CCnGG_A
Cuts at: 1055 3341 1055
Size: 2286 4898
PmeI GTTT'AAAC
Cuts at: 4183 4183
Size: 7184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3082 3114 6323 6355 3082
Size: 32 3209 32 3911
Fragments arranged by size:
3911 3209 32 32
PshAI GACnn'nnGTC
Cuts at: 1011 1011
Size: 7184
PsiI TTA'TAA
Cuts at: 206 206
Size: 7184
PspXI vC'TCGA_Gb
Cuts at: 3578 3578
Size: 7184
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 7152
PstI C_TGCA'G
Cuts at: 834 1913 3392 3702 4231 4916 834
Size: 1079 1479 310 529 685 3102
Fragments arranged by size:
3102 1479 1079 685 529 310
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7184
PvuII CAG'CTG
Cuts at: 232 1983 2632 2865 2926 3846 4609 4969 232
Size: 1751 649 233 61 920 763 360 2447
Fragments arranged by size:
2447 1751 920 763 649 360 233 61
RsrII CG'GwC_CG
Cuts at: 5379 5379
Size: 7184
SacI G_AGCT'C
Cuts at: 4198 4198
Size: 7184
SalI G'TCGA_C
Cuts at: 4221 4221
Size: 7184
SapI GCTCTTCn'nnn_
Cuts at: 2335 2636 5207 5417 2335
Size: 301 2571 210 4102
Fragments arranged by size:
4102 2571 301 210
SbfI CC_TGCA'GG
Cuts at: 4231 4231
Size: 7184
ScaI AGT'ACT
Cuts at: 2891 6701 2891
Size: 3810 3374
SexAI A'CCwGG_T
Cuts at: 867 867
Size: 7184
SfcI C'TryA_G
Cuts at: 33 191 830 1909 2280 2747 3388 3474 3543
Size: 158 639 1079 371 467 641 86 69
Cuts at: 3543 3698 3717 4227 4912 5905 6096 33
Size: 155 19 510 685 993 191 1121
Fragments arranged by size:
1121 1079 993 685 641 639 510 467
371 191 158 155 86 69 19
SfoI GGC'GCC
Cuts at: 4616 4864 4616
Size: 248 6936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7184
SmaI CCC'GGG
Cuts at: 4206 4206
Size: 7184
SmlI C'TyrA_G
Cuts at: 2297 2514 3578 4339 5254 5746 6008 2297
Size: 217 1064 761 915 492 262 3473
Fragments arranged by size:
3473 1064 915 761 492 262 217
SnaBI TAC'GTA
Cuts at: 2259 2259
Size: 7184
SpeI A'CTAG_T
Cuts at: 67 364 507 67
Size: 297 143 6744
Fragments arranged by size:
6744 297 143
SphI G_CATG'C
Cuts at: 3624 4237 4737 3624
Size: 613 500 6071
Fragments arranged by size:
6071 613 500
SspI AAT'ATT
Cuts at: 392 1467 392
Size: 1075 6109
StuI AGG'CCT
Cuts at: 330 2983 330
Size: 2653 4531
StyI C'CwwG_G
Cuts at: 157 1925 2006 3412 4318 4672 157
Size: 1768 81 1406 906 354 2669
Fragments arranged by size:
2669 1768 1406 906 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2552 2818 3039 3853 4144 4767 4900 5359 5568
Size: 266 221 814 291 623 133 459 209
Cuts at: 5568 2552
Size: 4168
Fragments arranged by size:
4168 814 623 459 291 266 221 209
133
TatI w'GTAC_w
Cuts at: 435 2889 3270 6699 435
Size: 2454 381 3429 920
Fragments arranged by size:
3429 2454 920 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1658 1690 2169 2201 3400 3432 3441 3473 6701
Size: 32 479 32 1199 32 9 32 3228
Cuts at: 6701 6733 1658
Size: 32 2109
Fragments arranged by size:
3228 2109 1199 479 32 32 32 32
32 9
Tth111I GACn'n_nGTC
Cuts at: 696 4131 4981 696
Size: 3435 850 2899
Fragments arranged by size:
3435 2899 850
XbaI T'CTAG_A
Cuts at: 3000 4215 3000
Size: 1215 5969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 933 4679 933
Size: 3746 3438
XhoI C'TCGA_G
Cuts at: 2297 3578 2297
Size: 1281 5903
XmaI C'CCGG_G
Cuts at: 4204 4204
Size: 7184
XmnI GAAnn'nnTTC
Cuts at: 1601 1828 4097 1601
Size: 227 2269 4688
Fragments arranged by size:
4688 2269 227
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PasI PciI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI ApaI AscI AvrII BbvCI BcgI
BmgBI BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI NotI NruI PacI
PflMI PmlI PpuMI PspOMI SacII SanDI SfiI SgrAI
SrfI SwaI ZraI