Restriction map for
pF1KA0455
(Circular) (Six-base) MAPSORT of: pF1KA0455.seq Check: 2520 from: 1 to: 5402
pF1KA0455 5402 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2440 2440
Size: 5402
Acc65I G'GTAC_C
Cuts at: 2418 2418
Size: 5402
AclI AA'CG_TT
Cuts at: 5242 5242
Size: 5402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1329 1916 2045 3227 3659 4012 4674 1329
Size: 587 129 1182 432 353 662 2057
Fragments arranged by size:
2057 1182 662 587 432 353 129
AflIII A'CryG_T
Cuts at: 2754 4554 4835 2754
Size: 1800 281 3321
Fragments arranged by size:
3321 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 631 631
Size: 5402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1372 1372
Size: 5402
AlwNI CAG_nnn'CTG
Cuts at: 814 2707 4145 814
Size: 1893 1438 2071
Fragments arranged by size:
2071 1893 1438
ApaLI G'TGCA_C
Cuts at: 205 397 1708 4240 205
Size: 192 1311 2532 1367
Fragments arranged by size:
2532 1367 1311 192
ApoI r'AATT_y
Cuts at: 533 780 1092 2406 4755 5011 533
Size: 247 312 1314 2349 256 924
Fragments arranged by size:
2349 1314 924 312 256 247
AseI AT'TA_AT
Cuts at: 20 590 20
Size: 570 4832
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
AvaI C'yCGr_G
Cuts at: 217 235 1766 2422 217
Size: 18 1531 656 3197
Fragments arranged by size:
3197 1531 656 18
BamHI G'GATC_C
Cuts at: 2427 2427
Size: 5402
BanI G'GyrC_C
Cuts at: 157 2418 2832 3080 3115 157
Size: 2261 414 248 35 2444
Fragments arranged by size:
2444 2261 414 248 35
BanII G_rGCy'C
Cuts at: 584 1318 1958 2096 2416 3446 584
Size: 734 640 138 320 1030 2540
Fragments arranged by size:
2540 1030 734 640 320 138
BbeI G_GCGC'C
Cuts at: 161 2836 3084 161
Size: 2675 248 2479
Fragments arranged by size:
2675 2479 248
BbsI GAAGACnn'nnnn_
Cuts at: 378 948 1391 378
Size: 570 443 4389
Fragments arranged by size:
4389 570 443
BbvCI CC'TCA_GC
Cuts at: 193 193
Size: 5402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 236 270 90
Size: 34 112 34 5222
Fragments arranged by size:
5222 112 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3300 4356 5402 3300
Size: 1056 1046 3300
Fragments arranged by size:
3300 1056 1046
BclI T'GATC_A
Cuts at: 284 2922 284
Size: 2638 2764
BglI GCCn_nnn'nGGC
Cuts at: 1662 1662
Size: 5402
BglII A'GATC_T
Cuts at: 2917 4954 2917
Size: 2037 3365
BlpI GC'TnA_GC
Cuts at: 484 2514 484
Size: 2030 3372
Bme1580I G_kGCm'C
Cuts at: 209 401 1712 3027 3120 4244 209
Size: 192 1311 1315 93 1124 1367
Fragments arranged by size:
1367 1315 1311 1124 192 93
BmrI ACTGGGnnnn_n'
Cuts at: 1015 1097 1449 1952 2221 2614 2695 3030 5174
Size: 82 352 503 269 393 81 335 2144
Cuts at: 5174 1015
Size: 1243
Fragments arranged by size:
2144 1243 503 393 352 335 269 82
81
BmtI G_CTAG'C
Cuts at: 1259 1259
Size: 5402
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1153 3769 1153
Size: 2616 2786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 196 617 1679 2174 108
Size: 88 421 1062 495 3336
Fragments arranged by size:
3336 1062 495 421 88
Bpu10I CC'TnA_GC
Cuts at: 193 807 1508 193
Size: 614 701 4087
Fragments arranged by size:
4087 701 614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2578 3457 4171 4469 2578
Size: 879 714 298 3511
Fragments arranged by size:
3511 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1148 1577 1914 5106 1148
Size: 429 337 3192 1444
Fragments arranged by size:
3192 1444 429 337
BsaAI yAC'GTr
Cuts at: 2755 2755
Size: 5402
BsaBI GATnn'nnATC
Cuts at: 6 2916 4953 6
Size: 2910 2037 455
Fragments arranged by size:
2910 2037 455
BsaHI Gr'CG_yC
Cuts at: 158 1133 2833 3081 3783 5279 158
Size: 975 1700 248 702 1496 281
Fragments arranged by size:
1700 1496 975 702 281 248
BsaWI w'CCGG_w
Cuts at: 422 2591 3112 3790 4201 4348 4622 422
Size: 2169 521 678 411 147 274 1202
Fragments arranged by size:
2169 1202 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 1067 1438 1735 193
Size: 874 371 297 3860
Fragments arranged by size:
3860 874 371 297
BseYI C'CCAG_C
Cuts at: 2827 3585 4250 4658 2827
Size: 758 665 408 3571
Fragments arranged by size:
3571 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1899 1899
Size: 5402
BsiEI CG_ry'CG
Cuts at: 104 2944 2990 4220 104
Size: 2840 46 1230 1286
Fragments arranged by size:
2840 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 209 401 1712 2096 2416 3194 3384 4244 209
Size: 192 1311 384 320 778 190 860 1367
Fragments arranged by size:
1367 1311 860 778 384 320 192 190
BsmI GAATG_Cn'
Cuts at: 1409 1409
Size: 5402
BsmBI CGTCTCn'nnnn_
Cuts at: 1124 2248 1124
Size: 1124 4278
Bsp1286I G_dGCh'C
Cuts at: 209 401 584 1318 1712 1958 2096 2416 3027
Size: 192 183 734 394 246 138 320 611
Cuts at: 3027 3120 3194 3384 3446 4244 209
Size: 93 74 190 62 798 1367
Fragments arranged by size:
1367 798 734 611 394 320 246 192
190 183 138 93 74 62
BspHI T'CATG_A
Cuts at: 3834 3834
Size: 5402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 513 2452 2666 2968 3349 513
Size: 1939 214 302 381 2566
Fragments arranged by size:
2566 1939 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1432 3694 3748 5232 5397 50
Size: 1382 2262 54 1484 165 55
Fragments arranged by size:
2262 1484 1382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2190 3314 2190
Size: 1124 4278
BsrFI r'CCGG_y
Cuts at: 3400 3581 3400
Size: 181 5221
BssHII G'CGCG_C
Cuts at: 114 114
Size: 5402
BssSI C'ACGA_G
Cuts at: 3673 4381 3673
Size: 708 4694
BstAPI GCAn_nnn'nTGC
Cuts at: 871 2779 871
Size: 1908 3494
BstBI TT'CG_AA
Cuts at: 3763 3763
Size: 5402
BstEII G'GTnAC_C
Cuts at: 1338 1338
Size: 5402
BstXI CCAn_nnnn'nTGG
Cuts at: 1597 1597
Size: 5402
BstYI r'GATC_y
Cuts at: 1 811 1164 1531 2012 2427 2894 2917 3252
Size: 810 353 367 481 415 467 23 335
Cuts at: 3252 3498 3804 3816 3902 4954 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 810 481 467 449 415 367 353
335 306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1138 1718 1138
Size: 580 4822
BtgI C'CryG_G
Cuts at: 135 1309 1319 1590 2199 135
Size: 1174 10 271 609 3338
Fragments arranged by size:
3338 1174 609 271 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3352 3533 3696 3352
Size: 181 163 5058
Fragments arranged by size:
5058 181 163
BtsI GCAGTG_nn'
Cuts at: 1209 2043 2645 1209
Size: 834 602 3966
Fragments arranged by size:
3966 834 602
DraI TTT'AAA
Cuts at: 2401 2401
Size: 5402
DraIII CAC_nnn'GTG
Cuts at: 1457 1457
Size: 5402
DrdI GACnn_nn'nnGTC
Cuts at: 3108 4452 3108
Size: 1344 4058
EaeI y'GGCC_r
Cuts at: 132 2987 3161 3552 3579 4924 132
Size: 2855 174 391 27 1345 610
Fragments arranged by size:
2855 1345 610 391 174 27
EagI C'GGCC_G
Cuts at: 2987 2987
Size: 5402
EarI CTCTTCn'nnn_
Cuts at: 3425 3635 3425
Size: 210 5192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3610 4352 4498 5212 3610
Size: 742 146 714 3800
Fragments arranged by size:
3800 742 714 146
EcoICRI GAG'CTC
Cuts at: 2094 2414 2094
Size: 320 5082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 196 617 1329 1679 1916 2045 2174 3227
Size: 88 421 712 350 237 129 129 1053
Cuts at: 3227 3659 4012 4674 108
Size: 432 353 662 836
Fragments arranged by size:
1053 836 712 662 432 421 353 350
237 129 129 88
EcoO109I rG'GnC_Cy
Cuts at: 1694 2541 1694
Size: 847 4555
EcoRI G'AATT_C
Cuts at: 533 2406 533
Size: 1873 3529
EcoRV GAT'ATC
Cuts at: 690 712 690
Size: 22 5380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1506 3421 1506
Size: 1915 3487
FspI TGC'GCA
Cuts at: 3183 3183
Size: 5402
HaeII r_GCGC'y
Cuts at: 161 168 1631 2836 3084 4314 5085 161
Size: 7 1463 1205 248 1230 771 478
Fragments arranged by size:
1463 1230 1205 771 478 248 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 617 649 790 803 822 835 1243 1275 2245
Size: 32 141 13 19 13 408 32 970
Cuts at: 2245 2277 3244 3274 3276 3306 617
Size: 32 967 30 2 30 2713
Fragments arranged by size:
2713 970 967 408 141 32 32 32
30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 2441 2441
Size: 5402
HindIII A'AGCT_T
Cuts at: 934 934
Size: 5402
KasI G'GCGC_C
Cuts at: 157 2832 3080 157
Size: 2675 248 2479
Fragments arranged by size:
2675 2479 248
KpnI G_GTAC'C
Cuts at: 2422 2422
Size: 5402
MluI A'CGCG_T
Cuts at: 4835 4835
Size: 5402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 552 4161 4345 552
Size: 3609 184 1609
Fragments arranged by size:
3609 1609 184
MscI TGG'CCA
Cuts at: 3163 3163
Size: 5402
MslI CAynn'nnrTG
Cuts at: 1250 1540 1849 3391 3518 1250
Size: 290 309 1542 127 3134
Fragments arranged by size:
3134 1542 309 290 127
MspA1I CmG'CkG
Cuts at: 128 137 155 197 699 886 2513 2827 3187
Size: 9 18 42 502 187 1627 314 360
Cuts at: 3187 3971 4216 128
Size: 784 245 1314
Fragments arranged by size:
1627 1314 784 502 360 314 245 187
42 18 9
NaeI GCC'GGC
Cuts at: 3583 3583
Size: 5402
NarI GG'CG_CC
Cuts at: 158 2833 3081 158
Size: 2675 248 2479
Fragments arranged by size:
2675 2479 248
NcoI C'CATG_G
Cuts at: 1319 1590 1319
Size: 271 5131
NgoMIV G'CCGG_C
Cuts at: 3581 3581
Size: 5402
NheI G'CTAG_C
Cuts at: 1255 1255
Size: 5402
NspI r_CATG'y
Cuts at: 438 865 1656 2455 2955 4558 438
Size: 427 791 799 500 1603 1282
Fragments arranged by size:
1603 1282 799 791 500 427
PciI A'CATG_T
Cuts at: 4554 4554
Size: 5402
PfoI T'CCnGG_A
Cuts at: 2044 2044
Size: 5402
PmeI GTTT'AAAC
Cuts at: 2401 2401
Size: 5402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 5370
PpuMI rG'GwC_Cy
Cuts at: 1694 1694
Size: 5402
PshAI GACnn'nnGTC
Cuts at: 425 425
Size: 5402
PsiI TTA'TAA
Cuts at: 2390 2390
Size: 5402
PstI C_TGCA'G
Cuts at: 2449 3134 2449
Size: 685 4717
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5402
PvuII CAG'CTG
Cuts at: 197 699 886 2827 3187 197
Size: 502 187 1941 360 2412
Fragments arranged by size:
2412 1941 502 360 187
RsrII CG'GwC_CG
Cuts at: 3597 3597
Size: 5402
SacI G_AGCT'C
Cuts at: 2096 2416 2096
Size: 320 5082
SacII CC_GC'GG
Cuts at: 138 138
Size: 5402
SalI G'TCGA_C
Cuts at: 2439 2439
Size: 5402
SapI GCTCTTCn'nnn_
Cuts at: 3425 3635 3425
Size: 210 5192
SbfI CC_TGCA'GG
Cuts at: 2449 2449
Size: 5402
ScaI AGT'ACT
Cuts at: 4919 4919
Size: 5402
SfcI C'TryA_G
Cuts at: 33 671 1379 2445 3130 4098 4289 33
Size: 638 708 1066 685 968 191 1146
Fragments arranged by size:
1146 1066 968 708 685 638 191
SfoI GGC'GCC
Cuts at: 159 2834 3082 159
Size: 2675 248 2479
Fragments arranged by size:
2675 2479 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
SmaI CCC'GGG
Cuts at: 219 237 2424 219
Size: 18 2187 3197
Fragments arranged by size:
3197 2187 18
SmlI C'TyrA_G
Cuts at: 2557 3472 4186 4448 2557
Size: 915 714 262 3511
Fragments arranged by size:
3511 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5402
SphI G_CATG'C
Cuts at: 438 1656 2455 2955 438
Size: 1218 799 500 2885
Fragments arranged by size:
2885 1218 799 500
StyI C'CwwG_G
Cuts at: 1319 1590 2536 2890 1319
Size: 271 946 354 3831
Fragments arranged by size:
3831 946 354 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1034 1468 1542 2000 2985 3118 3577 3786 1034
Size: 434 74 458 985 133 459 209 2650
Fragments arranged by size:
2650 985 459 458 434 209 133 74
TatI w'GTAC_w
Cuts at: 902 1067 4917 902
Size: 165 3850 1387
Fragments arranged by size:
3850 1387 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4919 4951 4919
Size: 32 5370
Tth111I GACn'n_nGTC
Cuts at: 295 1390 2259 3199 295
Size: 1095 869 940 2498
Fragments arranged by size:
2498 1095 940 869
XbaI T'CTAG_A
Cuts at: 2433 2433
Size: 5402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1594 1957 2897 1594
Size: 363 940 4099
Fragments arranged by size:
4099 940 363
XmaI C'CCGG_G
Cuts at: 217 235 2422 217
Size: 18 2187 3197
Fragments arranged by size:
3197 2187 18
XmnI GAAnn'nnTTC
Cuts at: 533 533
Size: 5402
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BsaXI BsiWI BspEI
BsrGI BstZ17I ClaI CspCI EcoNI FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PmlI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI