Restriction map for
pF1KA0449
(Circular) (Six-base) MAPSORT of: pF1KA0449.seq Check: 8186 from: 1 to: 7985
pF1KA0449 7985 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AatII G_ACGT'C
Cuts at: 3751 3751
Size: 7985
AccI GT'mk_AC
Cuts at: 5023 5023
Size: 7985
Acc65I G'GTAC_C
Cuts at: 5001 5001
Size: 7985
AclI AA'CG_TT
Cuts at: 7825 7825
Size: 7985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 967 2362 2814 3212 3380 5810 6242 6989
Size: 228 1395 452 398 168 2430 432 747
Cuts at: 6989 7257 739
Size: 268 1467
Fragments arranged by size:
2430 1467 1395 747 452 432 398 268
228 168
AfeI AGC'GCT
Cuts at: 3902 3902
Size: 7985
AflII C'TTAA_G
Cuts at: 2311 2311
Size: 7985
AflIII A'CryG_T
Cuts at: 5337 6441 7418 5337
Size: 1104 977 5904
Fragments arranged by size:
5904 1104 977
AgeI A'CCGG_T
Cuts at: 6373 6373
Size: 7985
AleI CACnn'nnGTG
Cuts at: 307 3115 3163 3805 3963 307
Size: 2808 48 642 158 4329
Fragments arranged by size:
4329 2808 642 158 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1280 1280
Size: 7985
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2941 2973 2941
Size: 32 7953
AlwNI CAG_nnn'CTG
Cuts at: 228 549 717 1749 2052 2277 2862 3039 3179
Size: 321 168 1032 303 225 585 177 140
Cuts at: 3179 4757 4886 5290 6857 228
Size: 1578 129 404 1567 1356
Fragments arranged by size:
1578 1567 1356 1032 585 404 321 303
225 177 168 140 129
ApaI G_GGCC'C
Cuts at: 732 1209 1407 2697 3571 732
Size: 477 198 1290 874 5146
Fragments arranged by size:
5146 1290 874 477 198
ApaLI G'TGCA_C
Cuts at: 822 1060 1265 3944 6755 822
Size: 238 205 2679 2811 2052
Fragments arranged by size:
2811 2679 2052 238 205
ApoI r'AATT_y
Cuts at: 4989 7338 7594 4989
Size: 2349 256 5380
Fragments arranged by size:
5380 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7985
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7985
AvaI C'yCGr_G
Cuts at: 210 447 882 1795 1941 1981 3013 3370 3707
Size: 237 435 913 146 40 1032 357 337
Cuts at: 3707 3734 4146 4233 4359 4631 4731 5005 210
Size: 27 412 87 126 272 100 274 3190
Fragments arranged by size:
3190 1032 913 435 412 357 337 274
272 237 146 126 100 87 40 27
AvrII C'CTAG_G
Cuts at: 3724 3724
Size: 7985
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 180 213 180
Size: 33 7952
BamHI G'GATC_C
Cuts at: 149 5010 149
Size: 4861 3124
BanI G'GyrC_C
Cuts at: 2576 2779 4339 4540 5001 5415 5663 5698 2576
Size: 203 1560 201 461 414 248 35 4863
Fragments arranged by size:
4863 1560 461 414 248 203 201 35
BanII G_rGCy'C
Cuts at: 221 557 732 775 1153 1209 1407 1648 1795
Size: 336 175 43 378 56 198 241 147
Cuts at: 1795 2508 2697 3013 3524 3571 4571 4676 4999
Size: 713 189 316 511 47 1000 105 323
Cuts at: 4999 6029 221
Size: 1030 2177
Fragments arranged by size:
2177 1030 1000 713 511 378 336 323
316 241 198 189 175 147 105 56
47 43
BbeI G_GCGC'C
Cuts at: 2580 5419 5667 2580
Size: 2839 248 4898
Fragments arranged by size:
4898 2839 248
BbsI GAAGACnn'nnnn_
Cuts at: 258 391 2557 3855 258
Size: 133 2166 1298 4388
Fragments arranged by size:
4388 2166 1298 133
BbvCI CC'TCA_GC
Cuts at: 1349 2182 2651 3044 4327 1349
Size: 833 469 393 1283 5007
Fragments arranged by size:
5007 1283 833 469 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 303 337 303
Size: 34 7951
BciVI GTATCCnnnnn_n'
Cuts at: 5883 6644 7985 5883
Size: 761 1341 5883
Fragments arranged by size:
5883 1341 761
BclI T'GATC_A
Cuts at: 1025 5505 1025
Size: 4480 3505
BglI GCCn_nnn'nGGC
Cuts at: 1204 1678 2362 2386 4337 4427 1204
Size: 474 684 24 1951 90 4762
Fragments arranged by size:
4762 1951 684 474 90 24
BglII A'GATC_T
Cuts at: 293 3434 5500 7537 293
Size: 3141 2066 2037 741
Fragments arranged by size:
3141 2066 2037 741
BlpI GC'TnA_GC
Cuts at: 4387 4750 5097 4387
Size: 363 347 7275
Fragments arranged by size:
7275 363 347
Bme1580I G_kGCm'C
Cuts at: 403 501 732 826 1064 1209 1269 1407 2697
Size: 98 231 94 238 145 60 138 1290
Cuts at: 2697 3571 3948 4342 4543 5610 5703 6759 403
Size: 874 377 394 201 1067 93 1056 1629
Fragments arranged by size:
1629 1290 1067 1056 874 394 377 238
231 201 145 138 98 94 93 60
BmgBI CAC'GTC
Cuts at: 4229 4229
Size: 7985
BmrI ACTGGGnnnn_n'
Cuts at: 537 866 3459 4716 5197 5278 5613 7757 537
Size: 329 2593 1257 481 81 335 2144 765
Fragments arranged by size:
2593 2144 1257 765 481 335 329 81
BmtI G_CTAG'C
Cuts at: 1501 1501
Size: 7985
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1005 1734 3165 3453 6352 1005
Size: 729 1431 288 2899 2638
Fragments arranged by size:
2899 2638 1431 729 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1084 1652 1771 2086 2176 2530 2691 2884
Size: 513 568 119 315 90 354 161 193
Cuts at: 2884 2935 3145 4863 571
Size: 51 210 1718 3693
Fragments arranged by size:
3693 1718 568 513 354 315 210 193
161 119 90 51
Bpu10I CC'TnA_GC
Cuts at: 1349 2182 2651 3044 4295 4327 1349
Size: 833 469 393 1251 32 5007
Fragments arranged by size:
5007 1251 833 469 393 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2080 2452 2833 3205 5161 6040 6532 6830 2080
Size: 372 381 372 1956 879 492 298 3235
Fragments arranged by size:
3235 1956 879 492 381 372 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 1168 2431 3124 3653 3687 4128 7689 1168
Size: 1263 693 529 34 441 3561 1464
Fragments arranged by size:
3561 1464 1263 693 529 441 34
BsaAI yAC'GTr
Cuts at: 5338 5338
Size: 7985
BsaBI GATnn'nnATC
Cuts at: 6 2038 5499 7536 6
Size: 2032 3461 2037 455
Fragments arranged by size:
3461 2037 2032 455
BsaHI Gr'CG_yC
Cuts at: 671 2577 3341 3748 5416 5664 6366 7862 671
Size: 1906 764 407 1668 248 702 1496 794
Fragments arranged by size:
1906 1668 1496 794 764 702 407 248
BsaWI w'CCGG_w
Cuts at: 5174 5695 6373 6647 6794 7205 5174
Size: 521 678 274 147 411 5954
Fragments arranged by size:
5954 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1063 1093 2941 2971 4108 4138 1063
Size: 30 1848 30 1137 30 4910
Fragments arranged by size:
4910 1848 1137 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 445 580 715 1087 1579 1861 1885 1888
Size: 3 135 135 372 492 282 24 3
Cuts at: 1888 1891 1921 1924 1999 2221 2425 2559 2965
Size: 3 30 3 75 222 204 134 406
Cuts at: 2965 3061 3142 3154 3306 3445 3947 442
Size: 96 81 12 152 139 502 4480
Fragments arranged by size:
4480 502 492 406 372 282 222 204
152 139 135 135 134 96 81 75
30 24 12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 158 231 827 1110 1466 4521 5410 6168 6745
Size: 73 596 283 356 3055 889 758 577
Cuts at: 6745 7241 158
Size: 496 902
Fragments arranged by size:
3055 902 889 758 596 577 496 356
283 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 2552 2636 3139 3418 4833 787
Size: 1765 84 503 279 1415 3939
Fragments arranged by size:
3939 1765 1415 503 279 84
BsiEI CG_ry'CG
Cuts at: 104 2166 3780 5527 5573 6781 104
Size: 2062 1614 1747 46 1208 1308
Fragments arranged by size:
2062 1747 1614 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 557 775 826 1064 1269 2300 2508 3948 4296
Size: 218 51 238 205 1031 208 1440 348
Cuts at: 4296 4676 4999 5777 5967 6759 557
Size: 380 323 778 190 792 1783
Fragments arranged by size:
1783 1440 1031 792 778 380 348 323
238 218 208 205 190 51
BsmI GAATG_Cn'
Cuts at: 1383 4214 1383
Size: 2831 5154
BsmBI CGTCTCn'nnnn_
Cuts at: 1843 1883 3739 3877 1843
Size: 40 1856 138 5951
Fragments arranged by size:
5951 1856 138 40
Bsp1286I G_dGCh'C
Cuts at: 221 403 501 557 732 775 826 1064 1153
Size: 182 98 56 175 43 51 238 89
Cuts at: 1153 1209 1269 1407 1648 1795 2300 2508 2697
Size: 56 60 138 241 147 505 208 189
Cuts at: 2697 3013 3524 3571 3948 4296 4342 4543 4571
Size: 316 511 47 377 348 46 201 28
Cuts at: 4571 4676 4999 5610 5703 5777 5967 6029 6759
Size: 105 323 611 93 74 190 62 730
Cuts at: 6759 221
Size: 1447
Fragments arranged by size:
1447 730 611 511 505 377 348 323
316 241 238 208 201 190 189 182
175 147 138 105 98 93 89 74
62 60 56 56 51 47 46 43
28
BspEI T'CCGG_A
Cuts at: 7205 7205
Size: 7985
BspHI T'CATG_A
Cuts at: 1133 1472 7161 1133
Size: 339 5689 1957
Fragments arranged by size:
5689 1957 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 608 1499 2276 4382 5035 5249 5551 5932 608
Size: 891 777 2106 653 214 302 381 2661
Fragments arranged by size:
2661 2106 891 777 653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 942 972 1755 2601 2699 2931 2994 6277
Size: 892 30 783 846 98 232 63 3283
Cuts at: 6277 6331 7815 7980 50
Size: 54 1484 165 55
Fragments arranged by size:
3283 1484 892 846 783 232 165 98
63 55 54 30
BsrDI GCAATG_nn'
Cuts at: 476 1026 1524 3855 3864 4371 5897 476
Size: 550 498 2331 9 507 1526 2564
Fragments arranged by size:
2564 2331 1526 550 507 498 9
BsrFI r'CCGG_y
Cuts at: 112 931 1507 5983 6164 6373 112
Size: 819 576 4476 181 209 1724
Fragments arranged by size:
4476 1724 819 576 209 181
BssSI C'ACGA_G
Cuts at: 637 4477 6256 6614 637
Size: 3840 1779 358 2008
Fragments arranged by size:
3840 2008 1779 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2277 2614 5362 2277
Size: 337 2748 4900
Fragments arranged by size:
4900 2748 337
BstBI TT'CG_AA
Cuts at: 6346 6346
Size: 7985
BstEII G'GTnAC_C
Cuts at: 4449 4449
Size: 7985
BstXI CCAn_nnnn'nTGG
Cuts at: 357 1707 3609 3807 4119 357
Size: 1350 1902 198 312 4223
Fragments arranged by size:
4223 1902 1350 312 198
BstYI r'GATC_y
Cuts at: 1 149 293 569 632 2138 2498 3269 3434
Size: 148 144 276 63 1506 360 771 165
Cuts at: 3434 5010 5477 5500 5835 6081 7093 7179 7191
Size: 1576 467 23 335 246 1012 86 12
Cuts at: 7191 7537 1
Size: 346 449
Fragments arranged by size:
1576 1506 1012 771 467 449 360 346
335 276 246 165 148 144 86 63
23 12
Bsu36I CC'TnA_GG
Cuts at: 1035 2542 1035
Size: 1507 6478
BtgI C'CryG_G
Cuts at: 107 350 395 1706 2189 3164 3974 107
Size: 243 45 1311 483 975 810 4118
Fragments arranged by size:
4118 1311 975 810 483 243 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 441 1526 5935 6116 6279 441
Size: 1085 4409 181 163 2147
Fragments arranged by size:
4409 2147 1085 181 163
BtsI GCAGTG_nn'
Cuts at: 902 1267 1953 2974 3958 3970 5228 902
Size: 365 686 1021 984 12 1258 3659
Fragments arranged by size:
3659 1258 1021 984 686 365 12
ClaI AT'CG_AT
Cuts at: 1442 1442
Size: 7985
DraI TTT'AAA
Cuts at: 4984 4984
Size: 7985
DraIII CAC_nnn'GTG
Cuts at: 921 921
Size: 7985
DrdI GACnn_nn'nnGTC
Cuts at: 130 3859 4227 5691 6549 130
Size: 3729 368 1464 858 1566
Fragments arranged by size:
3729 1566 1464 858 368
EaeI y'GGCC_r
Cuts at: 110 114 140 929 2015 2582 2660 3977 4419
Size: 4 26 789 1086 567 78 1317 442
Cuts at: 4419 4460 4949 5570 5744 6135 6162 7507 110
Size: 41 489 621 174 391 27 1345 588
Fragments arranged by size:
1345 1317 1086 789 621 588 567 489
442 391 174 78 41 27 26 4
EagI C'GGCC_G
Cuts at: 5570 5570
Size: 7985
EarI CTCTTCn'nnn_
Cuts at: 1783 1877 1946 2236 3014 6008 6218 1783
Size: 94 69 290 778 2994 210 3550
Fragments arranged by size:
3550 2994 778 290 210 94 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1882 2023 6193 6503 6649 7795 1882
Size: 141 4170 310 146 1146 2072
Fragments arranged by size:
4170 2072 1146 310 146 141
EcoICRI GAG'CTC
Cuts at: 555 773 2506 4674 4997 555
Size: 218 1733 2168 323 3543
Fragments arranged by size:
3543 2168 1733 323 218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 739 967 1084 1652 1771 2086 2176 2362
Size: 168 228 117 568 119 315 90 186
Cuts at: 2362 2530 2691 2814 2884 2935 3145 3212 3380
Size: 168 161 123 70 51 210 67 168
Cuts at: 3380 4863 5810 6242 6989 7257 571
Size: 1483 947 432 747 268 1299
Fragments arranged by size:
1483 1299 947 747 568 432 315 268
228 210 186 168 168 168 161 123
119 117 90 70 67 51
EcoNI CCTnn'n_nnAGG
Cuts at: 721 1638 2965 2968 721
Size: 917 1327 3 5738
Fragments arranged by size:
5738 1327 917 3
EcoO109I rG'GnC_Cy
Cuts at: 225 728 729 1403 1404 1746 2265 2693 3381
Size: 503 1 674 1 342 519 428 688
Cuts at: 3381 3568 4656 5124 225
Size: 187 1088 468 3086
Fragments arranged by size:
3086 1088 688 674 519 503 468 428
342 187 1 1
EcoRI G'AATT_C
Cuts at: 4989 4989
Size: 7985
EcoRV GAT'ATC
Cuts at: 3582 3582
Size: 7985
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 933 1824 4884 6004 933
Size: 891 3060 1120 2914
Fragments arranged by size:
3060 2914 1120 891
FseI GG_CCGG'CC
Cuts at: 116 116
Size: 7985
FspI TGC'GCA
Cuts at: 817 4697 5766 817
Size: 3880 1069 3036
Fragments arranged by size:
3880 3036 1069
FspAI rTGC'GCAy
Cuts at: 817 4697 817
Size: 3880 4105
HaeII r_GCGC'y
Cuts at: 495 542 1034 1349 2580 3904 4757 5419 5667
Size: 47 492 315 1231 1324 853 662 248
Cuts at: 5667 6689 7668 495
Size: 1022 979 812
Fragments arranged by size:
1324 1231 1022 979 853 812 662 492
315 248 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1924 1956 1957 1989 2490 2522 2671 2703 3667
Size: 32 1 32 501 32 149 32 964
Cuts at: 3667 3699 4073 4080 4105 4112 5827 5857 5859
Size: 32 374 7 25 7 1715 30 2
Cuts at: 5859 5889 1924
Size: 30 4020
Fragments arranged by size:
4020 1715 964 501 374 149 32 32
32 32 32 30 30 25 7 7
2 1
HincII GTy'rAC
Cuts at: 2910 5024 2910
Size: 2114 5871
HindIII A'AGCT_T
Cuts at: 1579 1579
Size: 7985
KasI G'GCGC_C
Cuts at: 2576 5415 5663 2576
Size: 2839 248 4898
Fragments arranged by size:
4898 2839 248
KpnI G_GTAC'C
Cuts at: 5005 5005
Size: 7985
MluI A'CGCG_T
Cuts at: 7418 7418
Size: 7985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 647 1854 3260 3909 6656 6840 404
Size: 243 1207 1406 649 2747 184 1549
Fragments arranged by size:
2747 1549 1406 1207 649 243 184
MscI TGG'CCA
Cuts at: 2584 4421 4951 5746 2584
Size: 1837 530 795 4823
Fragments arranged by size:
4823 1837 795 530
MslI CAynn'nnrTG
Cuts at: 307 757 2105 2887 3115 3163 3805 3963 4183
Size: 450 1348 782 228 48 642 158 220
Cuts at: 4183 4580 5974 6101 307
Size: 397 1394 127 2191
Fragments arranged by size:
2191 1394 1348 782 642 450 397 228
220 158 127 48
MspA1I CmG'CkG
Cuts at: 162 1782 2079 2154 2421 2481 3123 3756 5096
Size: 1620 297 75 267 60 642 633 1340
Cuts at: 5096 5410 5770 6783 7028 162
Size: 314 360 1013 245 1119
Fragments arranged by size:
1620 1340 1119 1013 642 633 360 314
297 267 245 75 60
NaeI GCC'GGC
Cuts at: 114 933 1509 6166 114
Size: 819 576 4657 1933
Fragments arranged by size:
4657 1933 819 576
NarI GG'CG_CC
Cuts at: 2577 5416 5664 2577
Size: 2839 248 4898
Fragments arranged by size:
4898 2839 248
NcoI C'CATG_G
Cuts at: 107 395 1706 2189 3974 107
Size: 288 1311 483 1785 4118
Fragments arranged by size:
4118 1785 1311 483 288
NgoMIV G'CCGG_C
Cuts at: 112 931 1507 6164 112
Size: 819 576 4657 1933
Fragments arranged by size:
4657 1933 819 576
NheI G'CTAG_C
Cuts at: 1497 1497
Size: 7985
NspI r_CATG'y
Cuts at: 822 5038 5538 6445 822
Size: 4216 500 907 2362
Fragments arranged by size:
4216 2362 907 500
PasI CC'CwG_GG
Cuts at: 1656 1656
Size: 7985
PciI A'CATG_T
Cuts at: 6441 6441
Size: 7985
PflMI CCAn_nnn'nTGG
Cuts at: 549 2748 549
Size: 2199 5786
PfoI T'CCnGG_A
Cuts at: 1100 1553 3335 4196 1100
Size: 453 1782 861 4889
Fragments arranged by size:
4889 1782 861 453
PmeI GTTT'AAAC
Cuts at: 4984 4984
Size: 7985
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2973 7124 7156 2941
Size: 32 4151 32 3770
Fragments arranged by size:
4151 3770 32 32
PpuMI rG'GwC_Cy
Cuts at: 225 1746 2265 4656 225
Size: 1521 519 2391 3554
Fragments arranged by size:
3554 2391 1521 519
PshAI GACnn'nnGTC
Cuts at: 2905 2905
Size: 7985
PspOMI G'GGCC_C
Cuts at: 728 1205 1403 2693 3567 728
Size: 477 198 1290 874 5146
Fragments arranged by size:
5146 1290 874 477 198
PspXI vC'TCGA_Gb
Cuts at: 882 882
Size: 7985
PstI C_TGCA'G
Cuts at: 1295 2132 2177 2273 2282 2969 3002 3035 3380
Size: 837 45 96 9 687 33 33 345
Cuts at: 3380 3965 4141 5032 5717 1295
Size: 585 176 891 685 3563
Fragments arranged by size:
3563 891 837 687 685 585 345 176
96 45 33 33 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7985
PvuII CAG'CTG
Cuts at: 162 2154 3123 5410 5770 162
Size: 1992 969 2287 360 2377
Fragments arranged by size:
2377 2287 1992 969 360
RsrII CG'GwC_CG
Cuts at: 6180 6180
Size: 7985
SacI G_AGCT'C
Cuts at: 557 775 2508 4676 4999 557
Size: 218 1733 2168 323 3543
Fragments arranged by size:
3543 2168 1733 323 218
SalI G'TCGA_C
Cuts at: 5022 5022
Size: 7985
SapI GCTCTTCn'nnn_
Cuts at: 1783 1877 6008 6218 1783
Size: 94 4131 210 3550
Fragments arranged by size:
4131 3550 210 94
SbfI CC_TGCA'GG
Cuts at: 2969 5032 2969
Size: 2063 5922
ScaI AGT'ACT
Cuts at: 4135 7502 4135
Size: 3367 4618
SexAI A'CCwGG_T
Cuts at: 836 836
Size: 7985
SfcI C'TryA_G
Cuts at: 33 1071 1291 1356 1387 2128 2173 2269 2278
Size: 1038 220 65 31 741 45 96 9
Cuts at: 2278 2350 2965 2998 3031 3376 3961 4137 5028
Size: 72 615 33 33 345 585 176 891
Cuts at: 5028 5713 6706 6897 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1038 993 891 741 685 615 585
345 220 191 176 96 72 65 45
33 33 31 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2386 4427 2386
Size: 2041 5944
SfoI GGC'GCC
Cuts at: 2578 5417 5665 2578
Size: 2839 248 4898
Fragments arranged by size:
4898 2839 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7985
SmaI CCC'GGG
Cuts at: 212 3709 4733 5007 212
Size: 3497 1024 274 3190
Fragments arranged by size:
3497 3190 1024 274
SmlI C'TyrA_G
Cuts at: 882 2095 2311 2467 2848 3220 3734 5140 6055
Size: 1213 216 156 381 372 514 1406 915
Cuts at: 6055 6547 6809 882
Size: 492 262 2058
Fragments arranged by size:
2058 1406 1213 915 514 492 381 372
262 216 156
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7985
SphI G_CATG'C
Cuts at: 5038 5538 5038
Size: 500 7485
StuI AGG'CCT
Cuts at: 247 247
Size: 7985
StyI C'CwwG_G
Cuts at: 107 395 977 1013 1502 1706 2189 2381 3134
Size: 288 582 36 489 204 483 192 753
Cuts at: 3134 3724 3938 3974 4493 5119 5473 107
Size: 590 214 36 519 626 354 2619
Fragments arranged by size:
2619 753 626 590 582 519 489 483
354 288 214 204 192 36 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 769 841 1175 1480 1648 2646 3794 4064 4468
Size: 72 334 305 168 998 1148 270 404
Cuts at: 4468 4698 4818 5568 5701 6160 6369 769
Size: 230 120 750 133 459 209 2385
Fragments arranged by size:
2385 1148 998 750 459 404 334 305
270 230 209 168 133 120 72
TatI w'GTAC_w
Cuts at: 4133 7500 4133
Size: 3367 4618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 604 636 4696 4728 7502 7534 604
Size: 32 4060 32 2774 32 1055
Fragments arranged by size:
4060 2774 1055 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 262 2884 3679 5782 262
Size: 2622 795 2103 2465
Fragments arranged by size:
2622 2465 2103 795
XbaI T'CTAG_A
Cuts at: 1584 5016 1584
Size: 3432 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1746 2910 3120 3141 4005 5480 1746
Size: 1164 210 21 864 1475 4251
Fragments arranged by size:
4251 1475 1164 864 210 21
XhoI C'TCGA_G
Cuts at: 882 3734 882
Size: 2852 5133
XmaI C'CCGG_G
Cuts at: 210 3707 4731 5005 210
Size: 3497 1024 274 3190
Fragments arranged by size:
3497 3190 1024 274
ZraI GAC'GTC
Cuts at: 3749 3749
Size: 7985
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI AhdI AscI BfrBI BsiWI BsrGI BssHII BstZ17I
CspCI HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PsiI PsrI SacII SanDI SgrAI SnaBI SrfI
SspI SwaI XmnI