Restriction map for
pF1KA0443
(Circular) (Six-base) MAPSORT of: pF1KA0443.seq Check: 8540 from: 1 to: 7298
pF1KA0443 7298 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 692 1386 2766 4336 692
Size: 694 1380 1570 3654
Fragments arranged by size:
3654 1570 1380 694
Acc65I G'GTAC_C
Cuts at: 4314 4314
Size: 7298
AclI AA'CG_TT
Cuts at: 7138 7138
Size: 7298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1451 1850 2357 2636 2771 2831 3053 3397
Size: 246 399 507 279 135 60 222 344
Cuts at: 3397 3587 4076 4295 5123 5555 6302 6570 1205
Size: 190 489 219 828 432 747 268 1933
Fragments arranged by size:
1933 828 747 507 489 432 399 344
279 268 246 222 219 190 135 60
AflIII A'CryG_T
Cuts at: 3193 4650 5754 6731 3193
Size: 1457 1104 977 3760
Fragments arranged by size:
3760 1457 1104 977
AgeI A'CCGG_T
Cuts at: 5686 5686
Size: 7298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2892 2892
Size: 7298
AlwNI CAG_nnn'CTG
Cuts at: 544 1828 2110 2425 2491 2809 3517 4603 6170
Size: 1284 282 315 66 318 708 1086 1567
Cuts at: 6170 544
Size: 1672
Fragments arranged by size:
1672 1567 1284 1086 708 318 315 282
66
ApaI G_GGCC'C
Cuts at: 932 932
Size: 7298
ApaLI G'TGCA_C
Cuts at: 6068 6068
Size: 7298
ApoI r'AATT_y
Cuts at: 742 1054 3479 3602 4011 4069 4228 4302 6651
Size: 312 2425 123 409 58 159 74 2349
Cuts at: 6651 6907 742
Size: 256 1133
Fragments arranged by size:
2425 2349 1133 409 312 256 159 123
74 58
AseI AT'TA_AT
Cuts at: 20 3592 20
Size: 3572 3726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7298
AvaI C'yCGr_G
Cuts at: 1274 3671 3737 4318 1274
Size: 2397 66 581 4254
Fragments arranged by size:
4254 2397 581 66
AvrII C'CTAG_G
Cuts at: 640 640
Size: 7298
BamHI G'GATC_C
Cuts at: 3609 4323 3609
Size: 714 6584
BanI G'GyrC_C
Cuts at: 133 4314 4728 4976 5011 133
Size: 4181 414 248 35 2420
Fragments arranged by size:
4181 2420 414 248 35
BanII G_rGCy'C
Cuts at: 396 842 932 1940 4312 5342 396
Size: 446 90 1008 2372 1030 2352
Fragments arranged by size:
2372 2352 1030 1008 446 90
BbeI G_GCGC'C
Cuts at: 4732 4980 4732
Size: 248 7050
BbsI GAAGACnn'nnnn_
Cuts at: 1019 2513 1019
Size: 1494 5804
BbvCI CC'TCA_GC
Cuts at: 1550 1643 1925 2111 2426 1550
Size: 93 282 186 315 6422
Fragments arranged by size:
6422 315 282 186 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1106 1140 1106
Size: 34 7264
BciVI GTATCCnnnnn_n'
Cuts at: 2174 2895 5196 5957 7298 2174
Size: 721 2301 761 1341 2174
Fragments arranged by size:
2301 2174 1341 761 721
BclI T'GATC_A
Cuts at: 1217 3420 4818 1217
Size: 2203 1398 3697
Fragments arranged by size:
3697 2203 1398
BglII A'GATC_T
Cuts at: 4813 6850 4813
Size: 2037 5261
BlpI GC'TnA_GC
Cuts at: 395 4410 395
Size: 4015 3283
Bme1580I G_kGCm'C
Cuts at: 138 330 932 4923 5016 6072 138
Size: 192 602 3991 93 1056 1364
Fragments arranged by size:
3991 1364 1056 602 192 93
BmrI ACTGGGnnnn_n'
Cuts at: 122 1463 1553 4510 4591 4926 7070 122
Size: 1341 90 2957 81 335 2144 350
Fragments arranged by size:
2957 2144 1341 350 335 90 81
BmtI G_CTAG'C
Cuts at: 1463 2518 1463
Size: 1055 6243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 456 1575 5665 456
Size: 1119 4090 2089
Fragments arranged by size:
4090 2089 1119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 738 1796 2032 2333 2483 308
Size: 430 1058 236 301 150 5123
Fragments arranged by size:
5123 1058 430 301 236 150
Bpu10I CC'TnA_GC
Cuts at: 1550 1643 1925 2111 2426 4235 1550
Size: 93 282 186 315 1809 4613
Fragments arranged by size:
4613 1809 315 282 186 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4474 5353 5845 6143 4474
Size: 879 492 298 5629
Fragments arranged by size:
5629 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 607 1645 2110 2338 2560 7002 607
Size: 1038 465 228 222 4442 903
Fragments arranged by size:
4442 1038 903 465 228 222
BsaAI yAC'GTr
Cuts at: 3194 4651 3194
Size: 1457 5841
BsaBI GATnn'nnATC
Cuts at: 6 4812 6849 6
Size: 4806 2037 455
Fragments arranged by size:
4806 2037 455
BsaHI Gr'CG_yC
Cuts at: 4729 4977 5679 7175 4729
Size: 248 702 1496 4852
Fragments arranged by size:
4852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2667 4487 5008 5686 5960 6107 6518 2667
Size: 1820 521 678 274 147 411 3447
Fragments arranged by size:
3447 1820 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 716 746 1015 1045 1387 1417 1769 1799 716
Size: 30 269 30 342 30 352 30 6215
Fragments arranged by size:
6215 352 342 269 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 625 1321 1942 1945 1948 2128 2215 2356 2443
Size: 696 621 3 3 180 87 141 87
Cuts at: 2443 2728 3259 625
Size: 285 531 4664
Fragments arranged by size:
4664 696 621 531 285 180 141 87
87 3 3
BseYI C'CCAG_C
Cuts at: 1075 2584 4723 5481 6058 6554 1075
Size: 1509 2139 758 577 496 1819
Fragments arranged by size:
2139 1819 1509 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 335
Size: 7298
BsiEI CG_ry'CG
Cuts at: 104 4840 4886 6094 104
Size: 4736 46 1208 1308
Fragments arranged by size:
4736 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 842 4312 5090 5280 6072 842
Size: 3470 778 190 792 2068
Fragments arranged by size:
3470 2068 792 778 190
BsmI GAATG_Cn'
Cuts at: 4210 4210
Size: 7298
Bsp1286I G_dGCh'C
Cuts at: 138 330 396 842 932 1940 4312 4923 5016
Size: 192 66 446 90 1008 2372 611 93
Cuts at: 5016 5090 5280 5342 6072 138
Size: 74 190 62 730 1364
Fragments arranged by size:
2372 1364 1008 730 611 446 192 190
93 90 74 66 62
BspEI T'CCGG_A
Cuts at: 2667 6518 2667
Size: 3851 3447
BspHI T'CATG_A
Cuts at: 3621 4031 6474 3621
Size: 410 2443 4445
Fragments arranged by size:
4445 2443 410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 941 3176 4348 4562 4864 5245 941
Size: 2235 1172 214 302 381 2994
Fragments arranged by size:
2994 2235 1172 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5590 5644 7128 7293 50
Size: 5540 54 1484 165 55
Fragments arranged by size:
5540 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1250 3647 3978 5210 1250
Size: 2397 331 1232 3338
Fragments arranged by size:
3338 2397 1232 331
BsrFI r'CCGG_y
Cuts at: 2170 5296 5477 5686 2170
Size: 3126 181 209 3782
Fragments arranged by size:
3782 3126 209 181
BssSI C'ACGA_G
Cuts at: 5569 5927 5569
Size: 358 6940
BstAPI GCAn_nnn'nTGC
Cuts at: 4675 4675
Size: 7298
BstBI TT'CG_AA
Cuts at: 5659 5659
Size: 7298
BstXI CCAn_nnnn'nTGG
Cuts at: 789 3372 789
Size: 2583 4715
BstYI r'GATC_y
Cuts at: 1 778 939 1000 2776 3609 4323 4790 4813
Size: 777 161 61 1776 833 714 467 23
Cuts at: 4813 5148 5394 6406 6492 6504 6850 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1776 1012 833 777 714 467 449 346
335 246 161 86 61 23 12
BstZ17I GTA'TAC
Cuts at: 2767 2767
Size: 7298
BtgI C'CryG_G
Cuts at: 3274 3274
Size: 7298
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5248 5429 5592 5248
Size: 181 163 6954
Fragments arranged by size:
6954 181 163
BtsI GCAGTG_nn'
Cuts at: 323 734 2681 4067 4541 323
Size: 411 1947 1386 474 3080
Fragments arranged by size:
3080 1947 1386 474 411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 299 334 299
Size: 35 7263
DraI TTT'AAA
Cuts at: 4297 4297
Size: 7298
DrdI GACnn_nn'nnGTC
Cuts at: 5004 5862 5004
Size: 858 6440
EaeI y'GGCC_r
Cuts at: 791 1688 2969 4883 5057 5448 5475 6820 791
Size: 897 1281 1914 174 391 27 1345 1269
Fragments arranged by size:
1914 1345 1281 1269 897 391 174 27
EagI C'GGCC_G
Cuts at: 4883 4883
Size: 7298
EarI CTCTTCn'nnn_
Cuts at: 161 1250 1430 1619 1643 1742 1805 1829 2018
Size: 1089 180 189 24 99 63 24 189
Cuts at: 2018 2051 2201 2285 2333 2402 2462 2558 2571
Size: 33 150 84 48 69 60 96 13
Cuts at: 2571 2612 2651 2807 2900 3563 4088 5321 5531
Size: 41 39 156 93 663 525 1233 210
Cuts at: 5531 161
Size: 1928
Fragments arranged by size:
1928 1233 1089 663 525 210 189 189
180 156 150 99 96 93 84 69
63 60 48 41 39 33 24 24
13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5506 5816 5962 7108 5506
Size: 310 146 1146 5696
Fragments arranged by size:
5696 1146 310 146
EcoICRI GAG'CTC
Cuts at: 840 4310 840
Size: 3470 3828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 738 1205 1451 1796 1850 2032 2333 2357
Size: 430 467 246 345 54 182 301 24
Cuts at: 2357 2483 2636 2771 2831 3053 3397 3587 4076
Size: 126 153 135 60 222 344 190 489
Cuts at: 4076 4295 5123 5555 6302 6570 308
Size: 219 828 432 747 268 1036
Fragments arranged by size:
1036 828 747 489 467 432 430 345
344 301 268 246 222 219 190 182
153 135 126 60 54 24
EcoNI CCTnn'n_nnAGG
Cuts at: 514 1206 514
Size: 692 6606
EcoO109I rG'GnC_Cy
Cuts at: 230 626 821 1325 1850 1949 2270 2543 2639
Size: 396 195 504 525 99 321 273 96
Cuts at: 2639 2735 2831 3116 4437 230
Size: 96 96 285 1321 3091
Fragments arranged by size:
3091 1321 525 504 396 321 285 273
195 99 96 96 96
EcoRI G'AATT_C
Cuts at: 4302 4302
Size: 7298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2151 3601 5317 2151
Size: 1450 1716 4132
Fragments arranged by size:
4132 1716 1450
FspI TGC'GCA
Cuts at: 5079 5079
Size: 7298
HaeII r_GCGC'y
Cuts at: 4732 4980 6002 6981 4732
Size: 248 1022 979 5049
Fragments arranged by size:
5049 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 378 410 716 748 859 891 1387
Size: 32 234 32 306 32 111 32 496
Cuts at: 1387 1419 2572 2604 3316 3348 3388 3420 3511
Size: 32 1153 32 712 32 40 32 91
Cuts at: 3511 3543 3879 3911 5140 5170 5172 5202 112
Size: 32 336 32 1229 30 2 30 2208
Fragments arranged by size:
2208 1229 1153 712 496 336 306 234
111 91 40 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1632 1995 4337 1632
Size: 363 2342 4593
Fragments arranged by size:
4593 2342 363
HindIII A'AGCT_T
Cuts at: 1121 1973 1121
Size: 852 6446
KasI G'GCGC_C
Cuts at: 4728 4976 4728
Size: 248 7050
KpnI G_GTAC'C
Cuts at: 4318 4318
Size: 7298
MluI A'CGCG_T
Cuts at: 6731 6731
Size: 7298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2164 5969 6153 2164
Size: 3805 184 3309
Fragments arranged by size:
3805 3309 184
MscI TGG'CCA
Cuts at: 793 2971 5059 793
Size: 2178 2088 3032
Fragments arranged by size:
3032 2178 2088
MslI CAynn'nnrTG
Cuts at: 787 3816 5287 5414 787
Size: 3029 1471 127 2671
Fragments arranged by size:
3029 2671 1471 127
MspA1I CmG'CkG
Cuts at: 1579 1718 2488 2521 3870 4409 4723 5083 6096
Size: 139 770 33 1349 539 314 360 1013
Cuts at: 6096 6341 1579
Size: 245 2536
Fragments arranged by size:
2536 1349 1013 770 539 360 314 245
139 33
NaeI GCC'GGC
Cuts at: 5479 5479
Size: 7298
NarI GG'CG_CC
Cuts at: 4729 4977 4729
Size: 248 7050
NcoI C'CATG_G
Cuts at: 3274 3274
Size: 7298
NdeI CA'TA_TG
Cuts at: 3817 3817
Size: 7298
NgoMIV G'CCGG_C
Cuts at: 5477 5477
Size: 7298
NheI G'CTAG_C
Cuts at: 1459 2514 1459
Size: 1055 6243
NspI r_CATG'y
Cuts at: 4351 4851 5758 4351
Size: 500 907 5891
Fragments arranged by size:
5891 907 500
PasI CC'CwG_GG
Cuts at: 932 932
Size: 7298
PciI A'CATG_T
Cuts at: 5754 5754
Size: 7298
PflMI CCAn_nnn'nTGG
Cuts at: 920 2044 920
Size: 1124 6174
PfoI T'CCnGG_A
Cuts at: 1726 3079 3473 1726
Size: 1353 394 5551
Fragments arranged by size:
5551 1353 394
PmeI GTTT'AAAC
Cuts at: 4297 4297
Size: 7298
PmlI CAC'GTG
Cuts at: 3194 3194
Size: 7298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2943 6437 6469 2911
Size: 32 3494 32 3740
Fragments arranged by size:
3740 3494 32 32
PpuMI rG'GwC_Cy
Cuts at: 230 626 821 1325 1850 1949 2270 2543 2639
Size: 396 195 504 525 99 321 273 96
Cuts at: 2639 2735 2831 3116 230
Size: 96 96 285 4412
Fragments arranged by size:
4412 525 504 396 321 285 273 195
99 96 96 96
PshAI GACnn'nnGTC
Cuts at: 2083 2083
Size: 7298
PspOMI G'GGCC_C
Cuts at: 928 928
Size: 7298
PstI C_TGCA'G
Cuts at: 4345 5030 4345
Size: 685 6613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7298
PvuII CAG'CTG
Cuts at: 1579 1718 2488 2521 3870 4723 5083 1579
Size: 139 770 33 1349 853 360 3794
Fragments arranged by size:
3794 1349 853 770 360 139 33
RsrII CG'GwC_CG
Cuts at: 5493 5493
Size: 7298
SacI G_AGCT'C
Cuts at: 842 4312 842
Size: 3470 3828
SalI G'TCGA_C
Cuts at: 4335 4335
Size: 7298
SapI GCTCTTCn'nnn_
Cuts at: 5321 5531 5321
Size: 210 7088
SbfI CC_TGCA'GG
Cuts at: 4345 4345
Size: 7298
ScaI AGT'ACT
Cuts at: 477 6815 477
Size: 6338 960
SexAI A'CCwGG_T
Cuts at: 919 1327 919
Size: 408 6890
SfcI C'TryA_G
Cuts at: 33 1148 1340 2285 3462 3965 4341 5026 6019
Size: 1115 192 945 1177 503 376 685 993
Cuts at: 6019 6210 33
Size: 191 1121
Fragments arranged by size:
1177 1121 1115 993 945 685 503 376
192 191
SfoI GGC'GCC
Cuts at: 4730 4978 4730
Size: 248 7050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7298
SmaI CCC'GGG
Cuts at: 4320 4320
Size: 7298
SmlI C'TyrA_G
Cuts at: 4453 5368 5860 6122 4453
Size: 915 492 262 5629
Fragments arranged by size:
5629 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7298
SphI G_CATG'C
Cuts at: 4351 4851 4351
Size: 500 6798
SspI AAT'ATT
Cuts at: 1844 4110 4130 4140 4191 1844
Size: 2266 20 10 51 4951
Fragments arranged by size:
4951 2266 51 20 10
StuI AGG'CCT
Cuts at: 645 3231 4079 645
Size: 2586 848 3864
Fragments arranged by size:
3864 2586 848
StyI C'CwwG_G
Cuts at: 221 275 347 640 1268 1477 3274 3500 4432
Size: 54 72 293 628 209 1797 226 932
Cuts at: 4432 4786 221
Size: 354 2733
Fragments arranged by size:
2733 1797 932 628 354 293 226 209
72 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 310 4881 5014 5473 5682 310
Size: 4571 133 459 209 1926
Fragments arranged by size:
4571 1926 459 209 133
TatI w'GTAC_w
Cuts at: 475 3508 6813 475
Size: 3033 3305 960
Fragments arranged by size:
3305 3033 960
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6815 6847 6815
Size: 32 7266
Tth111I GACn'n_nGTC
Cuts at: 1734 2215 5095 1734
Size: 481 2880 3937
Fragments arranged by size:
3937 2880 481
XbaI T'CTAG_A
Cuts at: 2935 4329 2935
Size: 1394 5904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 247 1389 4793 247
Size: 1142 3404 2752
Fragments arranged by size:
3404 2752 1142
XmaI C'CCGG_G
Cuts at: 4318 4318
Size: 7298
XmnI GAAnn'nnTTC
Cuts at: 3602 3657 3602
Size: 55 7243
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
BaeI BfrBI BglI BmgBI BsiWI BsmBI BsrGI BssHII
BstEII Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI
MfeI NotI NruI NsiI PacI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI