Restriction map for
pF1KA0437
(Circular) (Six-base) MAPSORT of: pF1KA0437.seq Check: 6980 from: 1 to: 7901
pF1KA0437 7901 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3464 3464
Size: 7901
AccI GT'mk_AC
Cuts at: 3802 4939 3802
Size: 1137 6764
Acc65I G'GTAC_C
Cuts at: 4917 4917
Size: 7901
AclI AA'CG_TT
Cuts at: 7741 7741
Size: 7901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1024 2197 3122 4312 4429 5726 6158 6511
Size: 588 1173 925 1190 117 1297 432 353
Cuts at: 6511 7173 436
Size: 662 1164
Fragments arranged by size:
1297 1190 1173 1164 925 662 588 432
353 117
AflII C'TTAA_G
Cuts at: 126 126
Size: 7901
AflIII A'CryG_T
Cuts at: 923 3944 5253 7053 7334 923
Size: 3021 1309 1800 281 1490
Fragments arranged by size:
3021 1800 1490 1309 281
AgeI A'CCGG_T
Cuts at: 712 712
Size: 7901
AhdI GACnn_n'nnGTC
Cuts at: 3147 3798 3147
Size: 651 7250
AleI CACnn'nnGTG
Cuts at: 2631 2631
Size: 7901
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4564 4564
Size: 7901
AlwNI CAG_nnn'CTG
Cuts at: 238 829 2967 5206 6644 238
Size: 591 2138 2239 1438 1495
Fragments arranged by size:
2239 2138 1495 1438 591
ApaLI G'TGCA_C
Cuts at: 1632 6739 1632
Size: 5107 2794
ApoI r'AATT_y
Cuts at: 376 462 1681 2047 4905 7254 7510 376
Size: 86 1219 366 2858 2349 256 767
Fragments arranged by size:
2858 2349 1219 767 366 256 86
AseI AT'TA_AT
Cuts at: 20 2246 3071 20
Size: 2226 825 4850
Fragments arranged by size:
4850 2226 825
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7901
AvaI C'yCGr_G
Cuts at: 4655 4751 4868 4921 4655
Size: 96 117 53 7635
Fragments arranged by size:
7635 117 96 53
AvrII C'CTAG_G
Cuts at: 3597 3597
Size: 7901
BamHI G'GATC_C
Cuts at: 248 1341 1488 3593 4926 248
Size: 1093 147 2105 1333 3223
Fragments arranged by size:
3223 2105 1333 1093 147
BanI G'GyrC_C
Cuts at: 1329 3093 3201 4662 4917 5331 5579 5614 1329
Size: 1764 108 1461 255 414 248 35 3616
Fragments arranged by size:
3616 1764 1461 414 255 248 108 35
BanII G_rGCy'C
Cuts at: 875 1082 1781 2482 2764 3698 4585 4601 4915
Size: 207 699 701 282 934 887 16 314
Cuts at: 4915 5945 875
Size: 1030 2831
Fragments arranged by size:
2831 1030 934 887 701 699 314 282
207 16
BbeI G_GCGC'C
Cuts at: 4666 5335 5583 4666
Size: 669 248 6984
Fragments arranged by size:
6984 669 248
BbsI GAAGACnn'nnnn_
Cuts at: 2095 2122 2411 3593 2095
Size: 27 289 1182 6403
Fragments arranged by size:
6403 1182 289 27
BbvCI CC'TCA_GC
Cuts at: 177 177
Size: 7901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4250 4284 4434 4468 4250
Size: 34 150 34 7683
Fragments arranged by size:
7683 150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1451 5799 6855 7901 1451
Size: 4348 1056 1046 1451
Fragments arranged by size:
4348 1451 1056 1046
BclI T'GATC_A
Cuts at: 1844 3078 5421 1844
Size: 1234 2343 4324
Fragments arranged by size:
4324 2343 1234
BfrBI ATG'CAT
Cuts at: 4541 4541
Size: 7901
BglI GCCn_nnn'nGGC
Cuts at: 1243 4660 1243
Size: 3417 4484
BglII A'GATC_T
Cuts at: 954 2147 5416 7453 954
Size: 1193 3269 2037 1402
Fragments arranged by size:
3269 2037 1402 1193
BlpI GC'TnA_GC
Cuts at: 3428 4790 5013 3428
Size: 1362 223 6316
Fragments arranged by size:
6316 1362 223
Bme1580I G_kGCm'C
Cuts at: 1239 1636 4175 5526 5619 6743 1239
Size: 397 2539 1351 93 1124 2397
Fragments arranged by size:
2539 2397 1351 1124 397 93
BmgBI CAC'GTC
Cuts at: 3945 3990 3945
Size: 45 7856
BmrI ACTGGGnnnn_n'
Cuts at: 2242 3230 4228 4488 5113 5194 5529 7673 2242
Size: 988 998 260 625 81 335 2144 2470
Fragments arranged by size:
2470 2144 998 988 625 335 260 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1551 2661 2778 6268 1551
Size: 1110 117 3490 3184
Fragments arranged by size:
3490 3184 1110 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 1795 1835 2515 2944 2998 3636 4088 4494
Size: 1580 40 680 429 54 638 452 406
Cuts at: 4494 4615 215
Size: 121 3501
Fragments arranged by size:
3501 1580 680 638 452 429 406 121
54 40
Bpu10I CC'TnA_GC
Cuts at: 177 300 2476 3664 4579 177
Size: 123 2176 1188 915 3499
Fragments arranged by size:
3499 2176 1188 915 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1281 2107 2890 3879 5077 5956 6670 6968
Size: 392 826 783 989 1198 879 714 298
Cuts at: 6968 889
Size: 1822
Fragments arranged by size:
1822 1198 989 879 826 783 714 392
298
BsaI GGTCTCn'nnnn_
Cuts at: 177 2191 4508 7605 177
Size: 2014 2317 3097 473
Fragments arranged by size:
3097 2317 2014 473
BsaAI yAC'GTr
Cuts at: 926 4395 5254 926
Size: 3469 859 3573
Fragments arranged by size:
3573 3469 859
BsaBI GATnn'nnATC
Cuts at: 6 496 502 1315 5415 7452 6
Size: 490 6 813 4100 2037 455
Fragments arranged by size:
4100 2037 813 490 455 6
BsaHI Gr'CG_yC
Cuts at: 2416 3821 4473 4663 5332 5580 6282 7778 2416
Size: 1405 652 190 669 248 702 1496 2539
Fragments arranged by size:
2539 1496 1405 702 669 652 248 190
BsaWI w'CCGG_w
Cuts at: 712 1307 3020 3418 3590 5090 5611 6289 6700
Size: 595 1713 398 172 1500 521 678 411
Cuts at: 6700 6847 7121 712
Size: 147 274 1492
Fragments arranged by size:
1713 1500 1492 678 595 521 411 398
274 172 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1768 1798 3385 3415 1768
Size: 30 1587 30 6254
Fragments arranged by size:
6254 1587 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 295 298 586 1079 2707 2995 4023 4083
Size: 130 3 288 493 1628 288 1028 60
Cuts at: 4083 4275 4852 165
Size: 192 577 3214
Fragments arranged by size:
3214 1628 1028 577 493 288 288 192
130 60 3
BseYI C'CCAG_C
Cuts at: 189 1012 2006 2704 3055 3315 3824 4552 4573
Size: 823 994 698 351 260 509 728 21
Cuts at: 4573 5326 6084 6749 7157 189
Size: 753 758 665 408 933
Fragments arranged by size:
994 933 823 758 753 728 698 665
509 408 351 260 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 935 3443 227
Size: 708 2508 4685
Fragments arranged by size:
4685 2508 708
BsiEI CG_ry'CG
Cuts at: 104 5443 5489 6719 104
Size: 5339 46 1230 1286
Fragments arranged by size:
5339 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1636 2547 2764 3698 4915 5693 5883 6743 1636
Size: 911 217 934 1217 778 190 860 2794
Fragments arranged by size:
2794 1217 934 911 860 778 217 190
BsmI GAATG_Cn'
Cuts at: 1182 3269 1182
Size: 2087 5814
BsmBI CGTCTCn'nnnn_
Cuts at: 1897 2689 1897
Size: 792 7109
Bsp1286I G_dGCh'C
Cuts at: 875 1082 1239 1636 1781 2482 2547 2764 3698
Size: 207 157 397 145 701 65 217 934
Cuts at: 3698 4175 4585 4601 4915 5526 5619 5693 5883
Size: 477 410 16 314 611 93 74 190
Cuts at: 5883 5945 6743 875
Size: 62 798 2033
Fragments arranged by size:
2033 934 798 701 611 477 410 397
314 217 207 190 157 145 93 74
65 62 16
BspEI T'CCGG_A
Cuts at: 1307 1307
Size: 7901
BspHI T'CATG_A
Cuts at: 1616 1911 3362 6333 1616
Size: 295 1451 2971 3184
Fragments arranged by size:
3184 2971 1451 295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3464 4556 4951 5165 5467 5848 3464
Size: 1092 395 214 302 381 5517
Fragments arranged by size:
5517 1092 395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 865 3672 4789 6193 6247 7731 7896 50
Size: 815 2807 1117 1404 54 1484 165 55
Fragments arranged by size:
2807 1484 1404 1117 815 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5813 5813
Size: 7901
BsrFI r'CCGG_y
Cuts at: 168 712 2846 2858 3757 4524 5899 6080 168
Size: 544 2134 12 899 767 1375 181 1989
Fragments arranged by size:
2134 1989 1375 899 767 544 181 12
BssSI C'ACGA_G
Cuts at: 3040 3924 6172 6880 3040
Size: 884 2248 708 4061
Fragments arranged by size:
4061 2248 884 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1234 1890 5278 1234
Size: 656 3388 3857
Fragments arranged by size:
3857 3388 656
BstBI TT'CG_AA
Cuts at: 3967 6262 3967
Size: 2295 5606
BstXI CCAn_nnnn'nTGG
Cuts at: 2610 2610
Size: 7901
BstYI r'GATC_y
Cuts at: 1 248 857 954 1341 1488 2147 3593 4364
Size: 247 609 97 387 147 659 1446 771
Cuts at: 4364 4403 4926 5393 5416 5751 5997 6303 6315
Size: 39 523 467 23 335 246 306 12
Cuts at: 6315 6401 7453 1
Size: 86 1052 449
Fragments arranged by size:
1446 1052 771 659 609 523 467 449
387 335 306 247 246 147 97 86
39 23 12
BtgI C'CryG_G
Cuts at: 107 875 1597 1610 107
Size: 768 722 13 6398
Fragments arranged by size:
6398 768 722 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2966 5851 6032 6195 2966
Size: 2885 181 163 4672
Fragments arranged by size:
4672 2885 181 163
BtsI GCAGTG_nn'
Cuts at: 918 1446 3528 3870 3909 4151 5144 918
Size: 528 2082 342 39 242 993 3675
Fragments arranged by size:
3675 2082 993 528 342 242 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3600 3635 3600
Size: 35 7866
DraI TTT'AAA
Cuts at: 4900 4900
Size: 7901
DrdI GACnn_nn'nnGTC
Cuts at: 5607 6951 5607
Size: 1344 6557
EaeI y'GGCC_r
Cuts at: 1751 2612 2853 3759 4625 5486 5660 6051 6078
Size: 861 241 906 866 861 174 391 27
Cuts at: 6078 7423 1751
Size: 1345 2229
Fragments arranged by size:
2229 1345 906 866 861 861 391 241
174 27
EagI C'GGCC_G
Cuts at: 5486 5486
Size: 7901
EarI CTCTTCn'nnn_
Cuts at: 362 2552 2752 4291 4456 4829 5924 6134 362
Size: 2190 200 1539 165 373 1095 210 2129
Fragments arranged by size:
2190 2129 1539 1095 373 210 200 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 275 4772 6109 6851 6997 7711 275
Size: 4497 1337 742 146 714 465
Fragments arranged by size:
4497 1337 742 714 465 146
EcoICRI GAG'CTC
Cuts at: 2762 3696 4913 2762
Size: 934 1217 5750
Fragments arranged by size:
5750 1217 934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 436 1024 1795 1835 2197 2515 2944 2998
Size: 221 588 771 40 362 318 429 54
Cuts at: 2998 3122 3636 4088 4312 4429 4494 4615 5726
Size: 124 514 452 224 117 65 121 1111
Cuts at: 5726 6158 6511 7173 215
Size: 432 353 662 943
Fragments arranged by size:
1111 943 771 662 588 514 452 432
429 362 353 318 224 221 124 121
117 65 54 40
EcoNI CCTnn'n_nnAGG
Cuts at: 700 4828 700
Size: 4128 3773
EcoO109I rG'GnC_Cy
Cuts at: 235 659 3463 3602 4782 4887 5040 235
Size: 424 2804 139 1180 105 153 3096
Fragments arranged by size:
3096 2804 1180 424 153 139 105
EcoRI G'AATT_C
Cuts at: 4905 4905
Size: 7901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1721 3798 5920 1721
Size: 2077 2122 3702
Fragments arranged by size:
3702 2122 2077
FspI TGC'GCA
Cuts at: 5682 5682
Size: 7901
HaeII r_GCGC'y
Cuts at: 4666 5335 5583 6813 7584 4666
Size: 669 248 1230 771 4983
Fragments arranged by size:
4983 1230 771 669 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 307 339 849 881 1513 1545 1768 1800 2782
Size: 32 510 32 632 32 223 32 982
Cuts at: 2782 2814 3133 3165 3784 3816 4101 4133 4492
Size: 32 319 32 619 32 285 32 359
Cuts at: 4492 4524 5743 5773 5775 5805 307
Size: 32 1219 30 2 30 2403
Fragments arranged by size:
2403 1219 982 632 619 510 359 319
285 223 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1452 4209 4940 1452
Size: 2757 731 4413
Fragments arranged by size:
4413 2757 731
HindIII A'AGCT_T
Cuts at: 3767 3767
Size: 7901
HpaI GTT'AAC
Cuts at: 1452 1452
Size: 7901
KasI G'GCGC_C
Cuts at: 4662 5331 5579 4662
Size: 669 248 6984
Fragments arranged by size:
6984 669 248
KpnI G_GTAC'C
Cuts at: 4921 4921
Size: 7901
MluI A'CGCG_T
Cuts at: 7334 7334
Size: 7901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 344 350 1369 1490 1651 2043 2060 2420 2575
Size: 6 1019 121 161 392 17 360 155
Cuts at: 2575 2852 3842 4250 6660 6844 344
Size: 277 990 408 2410 184 1401
Fragments arranged by size:
2410 1401 1019 990 408 392 360 277
184 161 155 121 17 6
MscI TGG'CCA
Cuts at: 1753 2614 2855 4627 5662 1753
Size: 861 241 1772 1035 3992
Fragments arranged by size:
3992 1772 1035 861 241
MslI CAynn'nnrTG
Cuts at: 1615 2631 3277 3412 4180 4621 4648 5890 6017
Size: 1016 646 135 768 441 27 1242 127
Cuts at: 6017 1615
Size: 3499
Fragments arranged by size:
3499 1242 1016 768 646 441 135 127
27
MspA1I CmG'CkG
Cuts at: 193 791 1599 2714 3702 4587 5012 5326 5686
Size: 598 808 1115 988 885 425 314 360
Cuts at: 5686 6470 6715 193
Size: 784 245 1379
Fragments arranged by size:
1379 1115 988 885 808 784 598 425
360 314 245
NaeI GCC'GGC
Cuts at: 3759 6082 3759
Size: 2323 5578
NarI GG'CG_CC
Cuts at: 4663 5332 5580 4663
Size: 669 248 6984
Fragments arranged by size:
6984 669 248
NcoI C'CATG_G
Cuts at: 107 1610 107
Size: 1503 6398
NgoMIV G'CCGG_C
Cuts at: 3757 6080 3757
Size: 2323 5578
NsiI A_TGCA'T
Cuts at: 4543 4543
Size: 7901
NspI r_CATG'y
Cuts at: 3492 4954 5454 7057 3492
Size: 1462 500 1603 4336
Fragments arranged by size:
4336 1603 1462 500
PasI CC'CwG_GG
Cuts at: 897 2206 897
Size: 1309 6592
PciI A'CATG_T
Cuts at: 7053 7053
Size: 7901
PflMI CCAn_nnn'nTGG
Cuts at: 238 829 2967 238
Size: 591 2138 5172
Fragments arranged by size:
5172 2138 591
PfoI T'CCnGG_A
Cuts at: 231 231
Size: 7901
PmeI GTTT'AAAC
Cuts at: 4900 4900
Size: 7901
PmlI CAC'GTG
Cuts at: 926 4395 926
Size: 3469 4432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 206 238 4150 4182 6347 6379 206
Size: 32 3912 32 2165 32 1728
Fragments arranged by size:
3912 2165 1728 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 235 3463 3602 4887 235
Size: 3228 139 1285 3249
Fragments arranged by size:
3249 3228 1285 139
PshAI GACnn'nnGTC
Cuts at: 1298 1298
Size: 7901
PstI C_TGCA'G
Cuts at: 3461 4263 4427 4948 5633 3461
Size: 802 164 521 685 5729
Fragments arranged by size:
5729 802 685 521 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7901
PvuII CAG'CTG
Cuts at: 193 5326 5686 193
Size: 5133 360 2408
Fragments arranged by size:
5133 2408 360
RsrII CG'GwC_CG
Cuts at: 6096 6096
Size: 7901
SacI G_AGCT'C
Cuts at: 2764 3698 4915 2764
Size: 934 1217 5750
Fragments arranged by size:
5750 1217 934
SacII CC_GC'GG
Cuts at: 1600 1600
Size: 7901
SalI G'TCGA_C
Cuts at: 4938 4938
Size: 7901
SanDI GG'GwC_CC
Cuts at: 3602 3602
Size: 7901
SapI GCTCTTCn'nnn_
Cuts at: 362 2552 2752 5924 6134 362
Size: 2190 200 3172 210 2129
Fragments arranged by size:
3172 2190 2129 210 200
SbfI CC_TGCA'GG
Cuts at: 3461 4948 3461
Size: 1487 6414
ScaI AGT'ACT
Cuts at: 7418 7418
Size: 7901
SfcI C'TryA_G
Cuts at: 33 3457 4259 4423 4944 5629 6597 6788 33
Size: 3424 802 164 521 685 968 191 1146
Fragments arranged by size:
3424 1146 968 802 685 521 191 164
SfoI GGC'GCC
Cuts at: 4664 5333 5581 4664
Size: 669 248 6984
Fragments arranged by size:
6984 669 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7901
SgrAI Cr'CCGG_yG
Cuts at: 712 712
Size: 7901
SmaI CCC'GGG
Cuts at: 4657 4923 4657
Size: 266 7635
SmlI C'TyrA_G
Cuts at: 126 868 1296 2122 2905 3858 5056 5971 6685
Size: 742 428 826 783 953 1198 915 714
Cuts at: 6685 6947 126
Size: 262 1080
Fragments arranged by size:
1198 1080 953 915 826 783 742 714
428 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7901
SphI G_CATG'C
Cuts at: 4954 5454 4954
Size: 500 7401
StuI AGG'CCT
Cuts at: 2358 3171 2358
Size: 813 7088
StyI C'CwwG_G
Cuts at: 107 1046 1415 1610 3294 3480 3512 3597 3842
Size: 939 369 195 1684 186 32 85 245
Cuts at: 3842 5035 5389 107
Size: 1193 354 2619
Fragments arranged by size:
2619 1684 1193 939 369 354 245 195
186 85 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 336 1026 1623 2453 2616 4507 5484 5617 6076
Size: 690 597 830 163 1891 977 133 459
Cuts at: 6076 6285 336
Size: 209 1952
Fragments arranged by size:
1952 1891 977 830 690 597 459 209
163 133
TatI w'GTAC_w
Cuts at: 3450 7416 3450
Size: 3966 3935
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1633 1665 3374 3385 3406 3417 7418 7450 1633
Size: 32 1709 11 21 11 4001 32 2084
Fragments arranged by size:
4001 2084 1709 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 5698 5698
Size: 7901
XbaI T'CTAG_A
Cuts at: 4932 4932
Size: 7901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1163 1383 4383 5396 1163
Size: 220 3000 1013 3668
Fragments arranged by size:
3668 3000 1013 220
XmaI C'CCGG_G
Cuts at: 4655 4921 4655
Size: 266 7635
XmnI GAAnn'nnTTC
Cuts at: 811 4300 811
Size: 3489 4412
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI ApaI AscI BaeI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI PacI
PsiI PspOMI PspXI PsrI SexAI SfiI SnaBI SrfI
SspI SwaI XhoI ZraI