Restriction map for
pF1KA0433
(Circular) (Six-base) MAPSORT of: pF1KA0433.seq Check: 315 from: 1 to: 6842
pF1KA0433 6842 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3672 3672
Size: 6842
AccI GT'mk_AC
Cuts at: 3658 3880 3658
Size: 222 6620
Acc65I G'GTAC_C
Cuts at: 3070 3858 3070
Size: 788 6054
AclI AA'CG_TT
Cuts at: 2681 6682 2681
Size: 4001 2841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 905 1065 1220 1680 1697 1972 2033 2461
Size: 348 160 155 460 17 275 61 428
Cuts at: 2461 4667 5099 5452 6114 557
Size: 2206 432 353 662 1285
Fragments arranged by size:
2206 1285 662 460 432 428 353 348
275 160 155 61 17
AflII C'TTAA_G
Cuts at: 1492 1492
Size: 6842
AflIII A'CryG_T
Cuts at: 1959 4194 5994 6275 1959
Size: 2235 1800 281 2526
Fragments arranged by size:
2526 2235 1800 281
AleI CACnn'nnGTG
Cuts at: 3307 3307
Size: 6842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3325 3325
Size: 6842
AlwNI CAG_nnn'CTG
Cuts at: 4147 5585 4147
Size: 1438 5404
ApaLI G'TGCA_C
Cuts at: 5680 5680
Size: 6842
ApoI r'AATT_y
Cuts at: 293 1095 1324 2462 2820 3541 3596 3846 6195
Size: 802 229 1138 358 721 55 250 2349
Cuts at: 6195 6451 293
Size: 256 684
Fragments arranged by size:
2349 1138 802 721 684 358 256 250
229 55
AseI AT'TA_AT
Cuts at: 20 982 20
Size: 962 5880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6842
AvaI C'yCGr_G
Cuts at: 3313 3862 3313
Size: 549 6293
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 830 863 830
Size: 33 6809
BamHI G'GATC_C
Cuts at: 2733 3867 2733
Size: 1134 5708
BanI G'GyrC_C
Cuts at: 2623 3070 3480 3858 4272 4520 4555 2623
Size: 447 410 378 414 248 35 4910
Fragments arranged by size:
4910 447 414 410 378 248 35
BanII G_rGCy'C
Cuts at: 2696 3040 3856 4886 2696
Size: 344 816 1030 4652
Fragments arranged by size:
4652 1030 816 344
BbeI G_GCGC'C
Cuts at: 4276 4524 4276
Size: 248 6594
BbsI GAAGACnn'nnnn_
Cuts at: 203 2095 2819 3019 3120 3592 203
Size: 1892 724 200 101 472 3453
Fragments arranged by size:
3453 1892 724 472 200 101
BciVI GTATCCnnnnn_n'
Cuts at: 1718 4740 5796 6842 1718
Size: 3022 1056 1046 1718
Fragments arranged by size:
3022 1718 1056 1046
BclI T'GATC_A
Cuts at: 4362 4362
Size: 6842
BfrBI ATG'CAT
Cuts at: 2067 2067
Size: 6842
BglII A'GATC_T
Cuts at: 3004 4357 6394 3004
Size: 1353 2037 3452
Fragments arranged by size:
3452 2037 1353
BlpI GC'TnA_GC
Cuts at: 3954 3954
Size: 6842
Bme1580I G_kGCm'C
Cuts at: 4467 4560 5684 4467
Size: 93 1124 5625
Fragments arranged by size:
5625 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3113 3833 4054 4135 4470 6614 3113
Size: 720 221 81 335 2144 3341
Fragments arranged by size:
3341 2144 720 335 221 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5209 5209
Size: 6842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 884 1733 2443 761
Size: 123 849 710 5160
Fragments arranged by size:
5160 849 710 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4018 4897 5611 5909 4018
Size: 879 714 298 4951
Fragments arranged by size:
4951 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2868 3284 6546 2868
Size: 416 3262 3164
Fragments arranged by size:
3262 3164 416
BsaAI yAC'GTr
Cuts at: 2212 3656 4195 2212
Size: 1444 539 4859
Fragments arranged by size:
4859 1444 539
BsaBI GATnn'nnATC
Cuts at: 6 1359 2716 4356 6393 6
Size: 1353 1357 1640 2037 455
Fragments arranged by size:
2037 1640 1357 1353 455
BsaHI Gr'CG_yC
Cuts at: 3108 3398 4273 4521 5223 6719 3108
Size: 290 875 248 702 1496 3231
Fragments arranged by size:
3231 1496 875 702 290 248
BsaWI w'CCGG_w
Cuts at: 4031 4552 5230 5641 5788 6062 4031
Size: 521 678 411 147 274 4811
Fragments arranged by size:
4811 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 361 1606 2438 220
Size: 141 1245 832 4624
Fragments arranged by size:
4624 1245 832 141
BseYI C'CCAG_C
Cuts at: 4267 5025 5690 6098 4267
Size: 758 665 408 5011
Fragments arranged by size:
5011 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 3767 698
Size: 3069 3773
BsiEI CG_ry'CG
Cuts at: 104 4384 4430 5660 104
Size: 4280 46 1230 1286
Fragments arranged by size:
4280 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2696 3314 3706 3856 4634 4824 5684 2696
Size: 618 392 150 778 190 860 3854
Fragments arranged by size:
3854 860 778 618 392 190 150
Bsp1286I G_dGCh'C
Cuts at: 2696 3040 3314 3706 3856 4467 4560 4634 4824
Size: 344 274 392 150 611 93 74 190
Cuts at: 4824 4886 5684 2696
Size: 62 798 3854
Fragments arranged by size:
3854 798 611 392 344 274 190 150
93 74 62
BspHI T'CATG_A
Cuts at: 1977 5274 1977
Size: 3297 3545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3236 3672 3892 4106 4408 4789 3236
Size: 436 220 214 302 381 5289
Fragments arranged by size:
5289 436 381 302 220 214
BsrBI CCG'CTC
Cuts at: 50 5134 5188 6672 6837 50
Size: 5084 54 1484 165 55
Fragments arranged by size:
5084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 792 2640 3554 4754 792
Size: 1848 914 1200 2880
Fragments arranged by size:
2880 1848 1200 914
BsrFI r'CCGG_y
Cuts at: 4840 5021 4840
Size: 181 6661
BsrGI T'GTAC_A
Cuts at: 2592 2592
Size: 6842
BssSI C'ACGA_G
Cuts at: 971 5113 5821 971
Size: 4142 708 1992
Fragments arranged by size:
4142 1992 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4219 4219
Size: 6842
BstBI TT'CG_AA
Cuts at: 2362 5203 2362
Size: 2841 4001
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 3096 3165 2317
Size: 779 69 5994
Fragments arranged by size:
5994 779 69
BstYI r'GATC_y
Cuts at: 1 305 2154 2733 2745 3004 3671 3867 4334
Size: 304 1849 579 12 259 667 196 467
Cuts at: 4334 4357 4692 4938 5244 5256 5342 6394 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1849 1052 667 579 467 449 335 306
304 259 246 196 86 23 12 12
BstZ17I GTA'TAC
Cuts at: 3659 3659
Size: 6842
Bsu36I CC'TnA_GG
Cuts at: 2390 2390
Size: 6842
BtgI C'CryG_G
Cuts at: 257 1177 3476 3580 257
Size: 920 2299 104 3519
Fragments arranged by size:
3519 2299 920 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4792 4973 5136 4792
Size: 181 163 6498
Fragments arranged by size:
6498 181 163
BtsI GCAGTG_nn'
Cuts at: 4085 4085
Size: 6842
DraI TTT'AAA
Cuts at: 318 2241 2678 2883 3527 3650 3841 318
Size: 1923 437 205 644 123 191 3319
Fragments arranged by size:
3319 1923 644 437 205 191 123
DrdI GACnn_nn'nnGTC
Cuts at: 4548 5892 4548
Size: 1344 5498
EaeI y'GGCC_r
Cuts at: 4427 4601 4992 5019 6364 4427
Size: 174 391 27 1345 4905
Fragments arranged by size:
4905 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4427 4427
Size: 6842
EarI CTCTTCn'nnn_
Cuts at: 338 812 1583 1599 1683 3026 3384 3512 4865
Size: 474 771 16 84 1343 358 128 1353
Cuts at: 4865 5075 338
Size: 210 2105
Fragments arranged by size:
2105 1353 1343 771 474 358 210 128
84 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5050 5792 5938 6652 5050
Size: 742 146 714 5240
Fragments arranged by size:
5240 742 714 146
EcoICRI GAG'CTC
Cuts at: 2694 3854 2694
Size: 1160 5682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 761 884 905 1065 1220 1680 1697 1733
Size: 204 123 21 160 155 460 17 36
Cuts at: 1733 1972 2033 2443 2461 4667 5099 5452 6114
Size: 239 61 410 18 2206 432 353 662
Cuts at: 6114 557
Size: 1285
Fragments arranged by size:
2206 1285 662 460 432 410 353 239
204 160 155 123 61 36 21 18
17
EcoNI CCTnn'n_nnAGG
Cuts at: 3272 3272
Size: 6842
EcoO109I rG'GnC_Cy
Cuts at: 3981 3981
Size: 6842
EcoRI G'AATT_C
Cuts at: 3846 3846
Size: 6842
EcoRV GAT'ATC
Cuts at: 1206 1206
Size: 6842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1018 1885 3755 4861 1018
Size: 867 1870 1106 2999
Fragments arranged by size:
2999 1870 1106 867
FspI TGC'GCA
Cuts at: 4623 4623
Size: 6842
HaeII r_GCGC'y
Cuts at: 4276 4524 5754 6525 4276
Size: 248 1230 771 4593
Fragments arranged by size:
4593 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1500 1532 1769 1801 1921 1935 1953 1967 2146
Size: 32 237 32 120 14 18 14 179
Cuts at: 2146 2178 2737 2769 4684 4714 4716 4746 1500
Size: 32 559 32 1915 30 2 30 3596
Fragments arranged by size:
3596 1915 559 237 179 120 32 32
32 32 30 30 18 14 14 2
HincII GTy'rAC
Cuts at: 1550 3881 1550
Size: 2331 4511
HindIII A'AGCT_T
Cuts at: 2026 2026
Size: 6842
KasI G'GCGC_C
Cuts at: 4272 4520 4272
Size: 248 6594
KpnI G_GTAC'C
Cuts at: 3074 3862 3074
Size: 788 6054
MfeI C'AATT_G
Cuts at: 1211 1211
Size: 6842
MluI A'CGCG_T
Cuts at: 6275 6275
Size: 6842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 114 225 5601 5785 114
Size: 111 5376 184 1171
Fragments arranged by size:
5376 1171 184 111
MscI TGG'CCA
Cuts at: 4603 4603
Size: 6842
MslI CAynn'nnrTG
Cuts at: 191 3307 4831 4958 191
Size: 3116 1524 127 2075
Fragments arranged by size:
3116 2075 1524 127
MspA1I CmG'CkG
Cuts at: 1831 3592 3953 4267 4627 5411 5656 1831
Size: 1761 361 314 360 784 245 3017
Fragments arranged by size:
3017 1761 784 361 360 314 245
NaeI GCC'GGC
Cuts at: 5023 5023
Size: 6842
NarI GG'CG_CC
Cuts at: 4273 4521 4273
Size: 248 6594
NcoI C'CATG_G
Cuts at: 257 1177 3476 257
Size: 920 2299 3623
Fragments arranged by size:
3623 2299 920
NgoMIV G'CCGG_C
Cuts at: 5021 5021
Size: 6842
NsiI A_TGCA'T
Cuts at: 2069 2069
Size: 6842
NspI r_CATG'y
Cuts at: 3895 4395 5998 3895
Size: 500 1603 4739
Fragments arranged by size:
4739 1603 500
PacI TTA_AT'TAA
Cuts at: 2075 2075
Size: 6842
PciI A'CATG_T
Cuts at: 5994 5994
Size: 6842
PflMI CCAn_nnn'nTGG
Cuts at: 3457 3457
Size: 6842
PfoI T'CCnGG_A
Cuts at: 159 1710 2790 159
Size: 1551 1080 4211
Fragments arranged by size:
4211 1551 1080
PmeI GTTT'AAAC
Cuts at: 3841 3841
Size: 6842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5288 5320 5288
Size: 32 6810
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1604 1636 1604
Size: 32 6810
PstI C_TGCA'G
Cuts at: 1662 1830 3889 4574 1662
Size: 168 2059 685 3930
Fragments arranged by size:
3930 2059 685 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6842
PvuII CAG'CTG
Cuts at: 1831 4267 4627 1831
Size: 2436 360 4046
Fragments arranged by size:
4046 2436 360
RsrII CG'GwC_CG
Cuts at: 5037 5037
Size: 6842
SacI G_AGCT'C
Cuts at: 2696 3856 2696
Size: 1160 5682
SalI G'TCGA_C
Cuts at: 3879 3879
Size: 6842
SapI GCTCTTCn'nnn_
Cuts at: 1683 4865 5075 1683
Size: 3182 210 3450
Fragments arranged by size:
3450 3182 210
SbfI CC_TGCA'GG
Cuts at: 1662 3889 1662
Size: 2227 4615
ScaI AGT'ACT
Cuts at: 6359 6359
Size: 6842
SfcI C'TryA_G
Cuts at: 33 1658 1773 1826 3272 3340 3605 3885 4570
Size: 1625 115 53 1446 68 265 280 685
Cuts at: 4570 5538 5729 33
Size: 968 191 1146
Fragments arranged by size:
1625 1446 1146 968 685 280 265 191
115 68 53
SfoI GGC'GCC
Cuts at: 4274 4522 4274
Size: 248 6594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6842
SmaI CCC'GGG
Cuts at: 3864 3864
Size: 6842
SmlI C'TyrA_G
Cuts at: 1492 3997 4912 5626 5888 1492
Size: 2505 915 714 262 2446
Fragments arranged by size:
2505 2446 915 714 262
SnaBI TAC'GTA
Cuts at: 2212 3656 2212
Size: 1444 5398
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6842
SphI G_CATG'C
Cuts at: 3895 4395 3895
Size: 500 6342
SspI AAT'ATT
Cuts at: 1140 1140
Size: 6842
StyI C'CwwG_G
Cuts at: 257 1177 3476 3976 4330 257
Size: 920 2299 500 354 2769
Fragments arranged by size:
2769 2299 920 500 354
SwaI ATTT'AAAT
Cuts at: 318 318
Size: 6842
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1890 3464 4425 4558 5017 5226 1890
Size: 1574 961 133 459 209 3506
Fragments arranged by size:
3506 1574 961 459 209 133
TatI w'GTAC_w
Cuts at: 2443 2592 6357 2443
Size: 149 3765 2928
Fragments arranged by size:
3765 2928 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2638 2670 6359 6391 2638
Size: 32 3689 32 3089
Fragments arranged by size:
3689 3089 32 32
Tth111I GACn'n_nGTC
Cuts at: 4639 4639
Size: 6842
XbaI T'CTAG_A
Cuts at: 2527 3498 3873 2527
Size: 971 375 5496
Fragments arranged by size:
5496 971 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 374 4337 374
Size: 3963 2879
XmaI C'CCGG_G
Cuts at: 3862 3862
Size: 6842
XmnI GAAnn'nnTTC
Cuts at: 252 644 1985 2046 2765 3506 252
Size: 392 1341 61 719 741 3588
Fragments arranged by size:
3588 1341 741 719 392 61
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PacI PciI PflMI PfoI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AloI ApaI AscI AvrII
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BspEI BssHII BstEII ClaI CspCI DraIII
FseI FspAI HpaI NdeI NheI NotI NruI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI StuI XhoI ZraI