Restriction map for
pF1KA0417
(Circular) (Six-base) MAPSORT of: pF1KA0417.seq Check: 6956 from: 1 to: 5138
pF1KA0417 5138 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AatII G_ACGT'C
Cuts at: 1808 1808
Size: 5138
AccI GT'mk_AC
Cuts at: 290 1070 2176 290
Size: 780 1106 3252
Fragments arranged by size:
3252 1106 780
Acc65I G'GTAC_C
Cuts at: 2154 2154
Size: 5138
AclI AA'CG_TT
Cuts at: 4978 4978
Size: 5138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 2963 3395 4142 4410 670
Size: 2293 432 747 268 1398
Fragments arranged by size:
2293 1398 747 432 268
AflII C'TTAA_G
Cuts at: 1111 1111
Size: 5138
AflIII A'CryG_T
Cuts at: 2490 3594 4571 2490
Size: 1104 977 3057
Fragments arranged by size:
3057 1104 977
AgeI A'CCGG_T
Cuts at: 3526 3526
Size: 5138
AleI CACnn'nnGTG
Cuts at: 2011 2011
Size: 5138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2029 2029
Size: 5138
AlwNI CAG_nnn'CTG
Cuts at: 507 648 2443 4010 507
Size: 141 1795 1567 1635
Fragments arranged by size:
1795 1635 1567 141
ApaI G_GGCC'C
Cuts at: 143 143
Size: 5138
ApaLI G'TGCA_C
Cuts at: 1797 3908 1797
Size: 2111 3027
ApoI r'AATT_y
Cuts at: 2142 4491 4747 2142
Size: 2349 256 2533
Fragments arranged by size:
2533 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5138
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
AvaI C'yCGr_G
Cuts at: 143 427 787 1516 1954 2158 143
Size: 284 360 729 438 204 3123
Fragments arranged by size:
3123 729 438 360 284 204
BamHI G'GATC_C
Cuts at: 483 2163 483
Size: 1680 3458
BanI G'GyrC_C
Cuts at: 714 1063 1566 1657 2013 2154 2568 2816 2851
Size: 349 503 91 356 141 414 248 35
Cuts at: 2851 714
Size: 3001
Fragments arranged by size:
3001 503 414 356 349 248 141 91
35
BanII G_rGCy'C
Cuts at: 143 2152 3182 143
Size: 2009 1030 2099
Fragments arranged by size:
2099 2009 1030
BbeI G_GCGC'C
Cuts at: 1570 2572 2820 1570
Size: 1002 248 3888
Fragments arranged by size:
3888 1002 248
BbsI GAAGACnn'nnnn_
Cuts at: 406 406
Size: 5138
BciVI GTATCCnnnnn_n'
Cuts at: 555 3036 3797 5138 555
Size: 2481 761 1341 555
Fragments arranged by size:
2481 1341 761 555
BclI T'GATC_A
Cuts at: 2658 2658
Size: 5138
BfrBI ATG'CAT
Cuts at: 347 347
Size: 5138
BglI GCCn_nnn'nGGC
Cuts at: 769 769
Size: 5138
BglII A'GATC_T
Cuts at: 2653 4690 2653
Size: 2037 3101
BlpI GC'TnA_GC
Cuts at: 877 2250 877
Size: 1373 3765
Bme1580I G_kGCm'C
Cuts at: 143 1364 1801 2018 2763 2856 3912 143
Size: 1221 437 217 745 93 1056 1369
Fragments arranged by size:
1369 1221 1056 745 437 217 93
BmgBI CAC'GTC
Cuts at: 1635 1635
Size: 5138
BmrI ACTGGGnnnn_n'
Cuts at: 554 2350 2431 2766 4910 554
Size: 1796 81 335 2144 782
Fragments arranged by size:
2144 1796 782 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 849 3505 849
Size: 2656 2482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 535 1042 161
Size: 374 507 4257
Fragments arranged by size:
4257 507 374
Bpu10I CC'TnA_GC
Cuts at: 944 944
Size: 5138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1636 2314 3193 3685 3983 1636
Size: 678 879 492 298 2791
Fragments arranged by size:
2791 879 678 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4842 4842
Size: 5138
BsaAI yAC'GTr
Cuts at: 1743 2491 1743
Size: 748 4390
BsaBI GATnn'nnATC
Cuts at: 6 1621 2652 4689 6
Size: 1615 1031 2037 455
Fragments arranged by size:
2037 1615 1031 455
BsaHI Gr'CG_yC
Cuts at: 1567 1805 2569 2817 3519 5015 1567
Size: 238 764 248 702 1496 1690
Fragments arranged by size:
1690 1496 764 702 248 238
BsaWI w'CCGG_w
Cuts at: 1091 1098 2327 2848 3526 3800 3947 4358 1091
Size: 7 1229 521 678 274 147 411 1871
Fragments arranged by size:
1871 1229 678 521 411 274 147 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 229 259 229
Size: 30 5108
BseYI C'CCAG_C
Cuts at: 928 1889 2563 3321 3898 4394 928
Size: 961 674 758 577 496 1672
Fragments arranged by size:
1672 961 758 674 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1609 520
Size: 1089 4049
BsiEI CG_ry'CG
Cuts at: 104 1705 2680 2726 3934 104
Size: 1601 975 46 1208 1308
Fragments arranged by size:
1601 1308 1208 975 46
BsiHKAI G_wGCw'C
Cuts at: 1376 1801 1928 2152 2930 3120 3912 1376
Size: 425 127 224 778 190 792 2602
Fragments arranged by size:
2602 792 778 425 224 190 127
BsmI GAATG_Cn'
Cuts at: 349 349
Size: 5138
BsmBI CGTCTCn'nnnn_
Cuts at: 258 258
Size: 5138
Bsp1286I G_dGCh'C
Cuts at: 143 1364 1376 1801 1928 2018 2152 2763 2856
Size: 1221 12 425 127 90 134 611 93
Cuts at: 2856 2930 3120 3182 3912 143
Size: 74 190 62 730 1369
Fragments arranged by size:
1369 1221 730 611 425 190 134 127
93 90 74 62 12
BspEI T'CCGG_A
Cuts at: 4358 4358
Size: 5138
BspHI T'CATG_A
Cuts at: 749 4314 749
Size: 3565 1573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2188 2402 2704 3085 2188
Size: 214 302 381 4241
Fragments arranged by size:
4241 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1710 3430 3484 4968 5133 50
Size: 1660 1720 54 1484 165 55
Fragments arranged by size:
1720 1660 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 809 1398 3050 809
Size: 589 1652 2897
Fragments arranged by size:
2897 1652 589
BsrFI r'CCGG_y
Cuts at: 124 735 1700 3136 3317 3526 124
Size: 611 965 1436 181 209 1736
Fragments arranged by size:
1736 1436 965 611 209 181
BssSI C'ACGA_G
Cuts at: 274 3409 3767 274
Size: 3135 358 1645
Fragments arranged by size:
3135 1645 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1380 1425 1587 2515 1380
Size: 45 162 928 4003
Fragments arranged by size:
4003 928 162 45
BstBI TT'CG_AA
Cuts at: 1172 2095 3499 1172
Size: 923 1404 2811
Fragments arranged by size:
2811 1404 923
BstEII G'GTnAC_C
Cuts at: 376 1094 376
Size: 718 4420
BstYI r'GATC_y
Cuts at: 1 483 1088 1147 1986 2030 2163 2630 2653
Size: 482 605 59 839 44 133 467 23
Cuts at: 2653 2988 3234 4246 4332 4344 4690 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 839 605 482 467 449 346 335
246 133 86 59 44 23 12
Bsu36I CC'TnA_GG
Cuts at: 824 824
Size: 5138
BtgI C'CryG_G
Cuts at: 107 557 1356 1682 107
Size: 450 799 326 3563
Fragments arranged by size:
3563 799 450 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 378 3088 3269 3432 378
Size: 2710 181 163 2084
Fragments arranged by size:
2710 2084 181 163
BtsI GCAGTG_nn'
Cuts at: 508 918 2006 2381 508
Size: 410 1088 375 3265
Fragments arranged by size:
3265 1088 410 375
ClaI AT'CG_AT
Cuts at: 1475 1475
Size: 5138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2061 2096 2061
Size: 35 5103
DraI TTT'AAA
Cuts at: 2137 2137
Size: 5138
DraIII CAC_nnn'GTG
Cuts at: 1368 1793 1368
Size: 425 4713
DrdI GACnn_nn'nnGTC
Cuts at: 250 1078 1684 2844 3702 250
Size: 828 606 1160 858 1686
Fragments arranged by size:
1686 1160 858 828 606
EaeI y'GGCC_r
Cuts at: 284 737 1877 2723 2897 3288 3315 4660 284
Size: 453 1140 846 174 391 27 1345 762
Fragments arranged by size:
1345 1140 846 762 453 391 174 27
EagI C'GGCC_G
Cuts at: 2723 2723
Size: 5138
EarI CTCTTCn'nnn_
Cuts at: 2092 3161 3371 2092
Size: 1069 210 3859
Fragments arranged by size:
3859 1069 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1151 1969 3346 3656 3802 4948 127
Size: 1024 818 1377 310 146 1146 317
Fragments arranged by size:
1377 1146 1024 818 317 310 146
EcoICRI GAG'CTC
Cuts at: 2150 2150
Size: 5138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 535 670 1042 2963 3395 4142 4410 161
Size: 374 135 372 1921 432 747 268 889
Fragments arranged by size:
1921 889 747 432 374 372 268 135
EcoO109I rG'GnC_Cy
Cuts at: 140 549 1500 2277 140
Size: 409 951 777 3001
Fragments arranged by size:
3001 951 777 409
EcoRI G'AATT_C
Cuts at: 2142 2142
Size: 5138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1557 3157 1557
Size: 1600 3538
FspI TGC'GCA
Cuts at: 2919 2919
Size: 5138
HaeII r_GCGC'y
Cuts at: 649 1570 2572 2820 3842 4821 649
Size: 921 1002 248 1022 979 966
Fragments arranged by size:
1022 1002 979 966 921 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 692 724 1068 1100 2980 3010 3012
Size: 32 431 32 344 32 1880 30 2
Cuts at: 3012 3042 229
Size: 30 2325
Fragments arranged by size:
2325 1880 431 344 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 291 1248 2177 291
Size: 957 929 3252
Fragments arranged by size:
3252 957 929
KasI G'GCGC_C
Cuts at: 1566 2568 2816 1566
Size: 1002 248 3888
Fragments arranged by size:
3888 1002 248
KpnI G_GTAC'C
Cuts at: 2158 2158
Size: 5138
MluI A'CGCG_T
Cuts at: 4571 4571
Size: 5138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 272 430 955 3809 3993 272
Size: 158 525 2854 184 1417
Fragments arranged by size:
2854 1417 525 184 158
MscI TGG'CCA
Cuts at: 2899 2899
Size: 5138
MslI CAynn'nnrTG
Cuts at: 352 712 1693 2011 3127 3254 352
Size: 360 981 318 1116 127 2236
Fragments arranged by size:
2236 1116 981 360 318 127
MspA1I CmG'CkG
Cuts at: 908 1308 1358 1684 1893 2249 2563 2923 3936
Size: 400 50 326 209 356 314 360 1013
Cuts at: 3936 4181 908
Size: 245 1865
Fragments arranged by size:
1865 1013 400 360 356 326 314 245
209 50
NaeI GCC'GGC
Cuts at: 126 737 3319 126
Size: 611 2582 1945
Fragments arranged by size:
2582 1945 611
NarI GG'CG_CC
Cuts at: 1567 2569 2817 1567
Size: 1002 248 3888
Fragments arranged by size:
3888 1002 248
NcoI C'CATG_G
Cuts at: 107 557 107
Size: 450 4688
NgoMIV G'CCGG_C
Cuts at: 124 735 3317 124
Size: 611 2582 1945
Fragments arranged by size:
2582 1945 611
NsiI A_TGCA'T
Cuts at: 349 349
Size: 5138
NspI r_CATG'y
Cuts at: 2191 2691 3598 2191
Size: 500 907 3731
Fragments arranged by size:
3731 907 500
PciI A'CATG_T
Cuts at: 3594 3594
Size: 5138
PflMI CCAn_nnn'nTGG
Cuts at: 480 503 1635 1839 480
Size: 23 1132 204 3779
Fragments arranged by size:
3779 1132 204 23
PfoI T'CCnGG_A
Cuts at: 1496 1496
Size: 5138
PmeI GTTT'AAAC
Cuts at: 2137 2137
Size: 5138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 421 453 4277 4309 421
Size: 32 3824 32 1250
Fragments arranged by size:
3824 1250 32 32
PpuMI rG'GwC_Cy
Cuts at: 549 1500 549
Size: 951 4187
PspOMI G'GGCC_C
Cuts at: 139 139
Size: 5138
PstI C_TGCA'G
Cuts at: 629 1239 1580 2185 2870 629
Size: 610 341 605 685 2897
Fragments arranged by size:
2897 685 610 605 341
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5138
PvuII CAG'CTG
Cuts at: 1893 2563 2923 1893
Size: 670 360 4108
Fragments arranged by size:
4108 670 360
RsrII CG'GwC_CG
Cuts at: 3333 3333
Size: 5138
SacI G_AGCT'C
Cuts at: 2152 2152
Size: 5138
SacII CC_GC'GG
Cuts at: 1359 1685 1359
Size: 326 4812
SalI G'TCGA_C
Cuts at: 289 2175 289
Size: 1886 3252
SapI GCTCTTCn'nnn_
Cuts at: 3161 3371 3161
Size: 210 4928
SbfI CC_TGCA'GG
Cuts at: 2185 2185
Size: 5138
ScaI AGT'ACT
Cuts at: 631 4655 631
Size: 4024 1114
SfcI C'TryA_G
Cuts at: 33 321 625 1235 1576 1914 2181 2866 3859
Size: 288 304 610 341 338 267 685 993
Cuts at: 3859 4050 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 610 341 338 304 288
267 191
SfoI GGC'GCC
Cuts at: 1568 2570 2818 1568
Size: 1002 248 3888
Fragments arranged by size:
3888 1002 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
SmaI CCC'GGG
Cuts at: 1956 2160 1956
Size: 204 4934
SmlI C'TyrA_G
Cuts at: 1111 1651 2293 3208 3700 3962 1111
Size: 540 642 915 492 262 2287
Fragments arranged by size:
2287 915 642 540 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5138
SphI G_CATG'C
Cuts at: 2191 2691 2191
Size: 500 4638
SspI AAT'ATT
Cuts at: 1193 1193
Size: 5138
StuI AGG'CCT
Cuts at: 776 776
Size: 5138
StyI C'CwwG_G
Cuts at: 107 332 557 815 2272 2626 107
Size: 225 225 258 1457 354 2619
Fragments arranged by size:
2619 1457 354 258 225 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1837 2721 2854 3313 3522 1837
Size: 884 133 459 209 3453
Fragments arranged by size:
3453 884 459 209 133
TatI w'GTAC_w
Cuts at: 629 4653 629
Size: 4024 1114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4655 4687 4655
Size: 32 5106
Tth111I GACn'n_nGTC
Cuts at: 1933 2935 1933
Size: 1002 4136
XbaI T'CTAG_A
Cuts at: 2169 2169
Size: 5138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2633 2633
Size: 5138
XmaI C'CCGG_G
Cuts at: 1954 2158 1954
Size: 204 4934
XmnI GAAnn'nnTTC
Cuts at: 1178 1178
Size: 5138
ZraI GAC'GTC
Cuts at: 1806 1806
Size: 5138
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AloI AscI AvrII BaeI BbvCI
BcgI BmtI BseRI BsiWI BsrGI BssHII BstXI BstZ17I
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PmlI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI