Restriction map for
pF1KA0412
(Circular) (Six-base) MAPSORT of: pF1KA0412.seq Check: 9203 from: 1 to: 4994
pF1KA0412 4994 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 2032 2032
Size: 4994
Acc65I G'GTAC_C
Cuts at: 2010 2010
Size: 4994
AclI AA'CG_TT
Cuts at: 4834 4834
Size: 4994
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 510 1512 1734 2819 3251 3998 4266 418
Size: 92 1002 222 1085 432 747 268 1146
Fragments arranged by size:
1146 1085 1002 747 432 268 222 92
AflIII A'CryG_T
Cuts at: 2346 3450 4427 2346
Size: 1104 977 2913
Fragments arranged by size:
2913 1104 977
AgeI A'CCGG_T
Cuts at: 3382 3382
Size: 4994
AleI CACnn'nnGTG
Cuts at: 1121 1121
Size: 4994
AlwNI CAG_nnn'CTG
Cuts at: 2299 3866 2299
Size: 1567 3427
ApaI G_GGCC'C
Cuts at: 138 1452 138
Size: 1314 3680
ApaLI G'TGCA_C
Cuts at: 1056 3764 1056
Size: 2708 2286
ApoI r'AATT_y
Cuts at: 776 1998 4347 4603 776
Size: 1222 2349 256 1167
Fragments arranged by size:
2349 1222 1167 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4994
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
AvaI C'yCGr_G
Cuts at: 1312 2014 1312
Size: 702 4292
BamHI G'GATC_C
Cuts at: 2019 2019
Size: 4994
BanI G'GyrC_C
Cuts at: 1187 1355 2010 2424 2672 2707 1187
Size: 168 655 414 248 35 3474
Fragments arranged by size:
3474 655 414 248 168 35
BanII G_rGCy'C
Cuts at: 138 254 574 1452 2008 3038 138
Size: 116 320 878 556 1030 2094
Fragments arranged by size:
2094 1030 878 556 320 116
BbeI G_GCGC'C
Cuts at: 2428 2676 2428
Size: 248 4746
BbsI GAAGACnn'nnnn_
Cuts at: 344 1152 1236 1833 1973 344
Size: 808 84 597 140 3365
Fragments arranged by size:
3365 808 597 140 84
BciVI GTATCCnnnnn_n'
Cuts at: 2892 3653 4994 2892
Size: 761 1341 2892
Fragments arranged by size:
2892 1341 761
BclI T'GATC_A
Cuts at: 1947 2514 1947
Size: 567 4427
BfrBI ATG'CAT
Cuts at: 1788 1788
Size: 4994
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4994
BglII A'GATC_T
Cuts at: 2509 4546 2509
Size: 2037 2957
BlpI GC'TnA_GC
Cuts at: 209 287 2106 209
Size: 78 1819 3097
Fragments arranged by size:
3097 1819 78
Bme1580I G_kGCm'C
Cuts at: 138 1060 1452 2619 2712 3768 138
Size: 922 392 1167 93 1056 1364
Fragments arranged by size:
1364 1167 1056 922 392 93
BmrI ACTGGGnnnn_n'
Cuts at: 878 1382 1802 2206 2287 2622 4766 878
Size: 504 420 404 81 335 2144 1106
Fragments arranged by size:
2144 1106 504 420 404 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 444 651 3361 444
Size: 207 2710 2077
Fragments arranged by size:
2710 2077 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 806 1058 1310 1478 1562 1692 1730 623
Size: 183 252 252 168 84 130 38 3887
Fragments arranged by size:
3887 252 252 183 168 130 84 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 2170 3049 3541 3839 1333
Size: 837 879 492 298 2488
Fragments arranged by size:
2488 879 837 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4698 4698
Size: 4994
BsaAI yAC'GTr
Cuts at: 2347 2347
Size: 4994
BsaBI GATnn'nnATC
Cuts at: 6 2508 4545 6
Size: 2502 2037 455
Fragments arranged by size:
2502 2037 455
BsaHI Gr'CG_yC
Cuts at: 2425 2673 3375 4871 2425
Size: 248 702 1496 2548
Fragments arranged by size:
2548 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2183 2704 3382 3656 3803 4214 2183
Size: 521 678 274 147 411 2963
Fragments arranged by size:
2963 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 635 665 635
Size: 30 4964
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 199
Size: 4994
BseYI C'CCAG_C
Cuts at: 932 2419 3177 3754 4250 932
Size: 1487 758 577 496 1676
Fragments arranged by size:
1676 1487 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1245 1277 1413 1245
Size: 32 136 4826
Fragments arranged by size:
4826 136 32
BsiEI CG_ry'CG
Cuts at: 104 2536 2582 3790 104
Size: 2432 46 1208 1308
Fragments arranged by size:
2432 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 838 1060 1678 2008 2786 2976 3768 838
Size: 222 618 330 778 190 792 2064
Fragments arranged by size:
2064 792 778 618 330 222 190
BsmI GAATG_Cn'
Cuts at: 808 985 808
Size: 177 4817
Bsp1286I G_dGCh'C
Cuts at: 138 254 574 838 1060 1452 1678 2008 2619
Size: 116 320 264 222 392 226 330 611
Cuts at: 2619 2712 2786 2976 3038 3768 138
Size: 93 74 190 62 730 1364
Fragments arranged by size:
1364 730 611 392 330 320 264 226
222 190 116 93 74 62
BspEI T'CCGG_A
Cuts at: 4214 4214
Size: 4994
BspHI T'CATG_A
Cuts at: 4170 4170
Size: 4994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2044 2258 2560 2941 2044
Size: 214 302 381 4097
Fragments arranged by size:
4097 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1004 3286 3340 4824 4989 50
Size: 954 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 954 165 55 54
BsrDI GCAATG_nn'
Cuts at: 981 2906 981
Size: 1925 3069
BsrFI r'CCGG_y
Cuts at: 2992 3173 3382 2992
Size: 181 209 4604
Fragments arranged by size:
4604 209 181
BssSI C'ACGA_G
Cuts at: 1602 3265 3623 1602
Size: 1663 358 2973
Fragments arranged by size:
2973 1663 358
BstAPI GCAn_nnn'nTGC
Cuts at: 980 2371 980
Size: 1391 3603
BstBI TT'CG_AA
Cuts at: 3355 3355
Size: 4994
BstYI r'GATC_y
Cuts at: 1 540 684 2019 2486 2509 2844 3090 4102
Size: 539 144 1335 467 23 335 246 1012
Cuts at: 4102 4188 4200 4546 1
Size: 86 12 346 449
Fragments arranged by size:
1335 1012 539 467 449 346 335 246
144 86 23 12
BtgI C'CryG_G
Cuts at: 107 322 107
Size: 215 4779
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2944 3125 3288 2944
Size: 181 163 4650
Fragments arranged by size:
4650 181 163
BtsI GCAGTG_nn'
Cuts at: 729 1233 1401 1419 2237 729
Size: 504 168 18 818 3486
Fragments arranged by size:
3486 818 504 168 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1467 1502 1467
Size: 35 4959
DraI TTT'AAA
Cuts at: 717 1993 717
Size: 1276 3718
DrdI GACnn_nn'nnGTC
Cuts at: 2700 3558 2700
Size: 858 4136
EaeI y'GGCC_r
Cuts at: 1068 2579 2753 3144 3171 4516 1068
Size: 1511 174 391 27 1345 1546
Fragments arranged by size:
1546 1511 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2579 2579
Size: 4994
EarI CTCTTCn'nnn_
Cuts at: 695 3017 3227 695
Size: 2322 210 2462
Fragments arranged by size:
2462 2322 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3202 3512 3658 4804 3202
Size: 310 146 1146 3392
Fragments arranged by size:
3392 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2006 2006
Size: 4994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 510 623 806 1058 1310 1478 1512 1562
Size: 92 113 183 252 252 168 34 50
Cuts at: 1562 1692 1730 1734 2819 3251 3998 4266 418
Size: 130 38 4 1085 432 747 268 1146
Fragments arranged by size:
1146 1085 747 432 268 252 252 183
168 130 113 92 50 38 34 4
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 4994
EcoO109I rG'GnC_Cy
Cuts at: 878 1448 1512 2133 878
Size: 570 64 621 3739
Fragments arranged by size:
3739 621 570 64
EcoRI G'AATT_C
Cuts at: 1998 1998
Size: 4994
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1263 3013 1263
Size: 1750 3244
FspI TGC'GCA
Cuts at: 2775 2775
Size: 4994
HaeII r_GCGC'y
Cuts at: 2428 2676 3698 4677 2428
Size: 248 1022 979 2745
Fragments arranged by size:
2745 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 635 667 825 857 1331 1363 1915
Size: 32 116 32 158 32 474 32 552
Cuts at: 1915 1947 2836 2866 2868 2898 487
Size: 32 889 30 2 30 2583
Fragments arranged by size:
2583 889 552 474 158 116 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2033 2033
Size: 4994
KasI G'GCGC_C
Cuts at: 2424 2672 2424
Size: 248 4746
KpnI G_GTAC'C
Cuts at: 2014 2014
Size: 4994
MluI A'CGCG_T
Cuts at: 4427 4427
Size: 4994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3665 3849 3665
Size: 184 4810
MscI TGG'CCA
Cuts at: 1070 2755 1070
Size: 1685 3309
MslI CAynn'nnrTG
Cuts at: 1121 1274 1588 2983 3110 1121
Size: 153 314 1395 127 3005
Fragments arranged by size:
3005 1395 314 153 127
MspA1I CmG'CkG
Cuts at: 118 198 213 598 942 1362 2105 2419 2779
Size: 80 15 385 344 420 743 314 360
Cuts at: 2779 3792 4037 118
Size: 1013 245 1075
Fragments arranged by size:
1075 1013 743 420 385 360 344 314
245 80 15
NaeI GCC'GGC
Cuts at: 3175 3175
Size: 4994
NarI GG'CG_CC
Cuts at: 2425 2673 2425
Size: 248 4746
NcoI C'CATG_G
Cuts at: 107 322 107
Size: 215 4779
NgoMIV G'CCGG_C
Cuts at: 3173 3173
Size: 4994
NsiI A_TGCA'T
Cuts at: 1790 1790
Size: 4994
NspI r_CATG'y
Cuts at: 1593 1696 2047 2547 3454 1593
Size: 103 351 500 907 3133
Fragments arranged by size:
3133 907 500 351 103
PciI A'CATG_T
Cuts at: 3450 3450
Size: 4994
PfoI T'CCnGG_A
Cuts at: 639 639
Size: 4994
PmeI GTTT'AAAC
Cuts at: 1993 1993
Size: 4994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 4133
Size: 32 4962
PpuMI rG'GwC_Cy
Cuts at: 1512 1512
Size: 4994
PshAI GACnn'nnGTC
Cuts at: 356 356
Size: 4994
PspOMI G'GGCC_C
Cuts at: 134 1448 134
Size: 1314 3680
PspXI vC'TCGA_Gb
Cuts at: 1312 1312
Size: 4994
PstI C_TGCA'G
Cuts at: 2041 2726 2041
Size: 685 4309
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4994
PvuII CAG'CTG
Cuts at: 198 598 2419 2779 198
Size: 400 1821 360 2413
Fragments arranged by size:
2413 1821 400 360
RsrII CG'GwC_CG
Cuts at: 129 3189 129
Size: 3060 1934
SacI G_AGCT'C
Cuts at: 2008 2008
Size: 4994
SalI G'TCGA_C
Cuts at: 2031 2031
Size: 4994
SapI GCTCTTCn'nnn_
Cuts at: 3017 3227 3017
Size: 210 4784
SbfI CC_TGCA'GG
Cuts at: 2041 2041
Size: 4994
ScaI AGT'ACT
Cuts at: 4511 4511
Size: 4994
SfcI C'TryA_G
Cuts at: 33 2037 2722 3715 3906 33
Size: 2004 685 993 191 1121
Fragments arranged by size:
2004 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2426 2674 2426
Size: 248 4746
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
SmaI CCC'GGG
Cuts at: 2016 2016
Size: 4994
SmlI C'TyrA_G
Cuts at: 1312 1348 2149 3064 3556 3818 1312
Size: 36 801 915 492 262 2488
Fragments arranged by size:
2488 915 801 492 262 36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4994
SphI G_CATG'C
Cuts at: 2047 2547 2047
Size: 500 4494
SspI AAT'ATT
Cuts at: 1914 1914
Size: 4994
StuI AGG'CCT
Cuts at: 907 991 1411 1579 1747 907
Size: 84 420 168 168 4154
Fragments arranged by size:
4154 420 168 168 84
StyI C'CwwG_G
Cuts at: 107 179 322 2128 2482 107
Size: 72 143 1806 354 2619
Fragments arranged by size:
2619 1806 354 143 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1012 1432 2577 2710 3169 3378 1012
Size: 420 1145 133 459 209 2628
Fragments arranged by size:
2628 1145 459 420 209 133
TatI w'GTAC_w
Cuts at: 4509 4509
Size: 4994
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 446 478 936 968 1608 1640 4511 4543 446
Size: 32 458 32 640 32 2871 32 897
Fragments arranged by size:
2871 897 640 458 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2791 2791
Size: 4994
XbaI T'CTAG_A
Cuts at: 2025 2025
Size: 4994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 307 2489 307
Size: 2182 2812
XhoI C'TCGA_G
Cuts at: 1312 1312
Size: 4994
XmaI C'CCGG_G
Cuts at: 2014 2014
Size: 4994
XmnI GAAnn'nnTTC
Cuts at: 1243 1327 1384 1243
Size: 84 57 4853
Fragments arranged by size:
4853 84 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PsiI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI ZraI