Restriction map for
pF1KA0409
(Circular) (Six-base) MAPSORT of: pF1KA0409.seq Check: 9781 from: 1 to: 4481
pF1KA0409 4481 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 1519 1519
Size: 4481
Acc65I G'GTAC_C
Cuts at: 1497 1497
Size: 4481
AclI AA'CG_TT
Cuts at: 4321 4321
Size: 4481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 509 1273 1435 2306 2738 3485 3753 386
Size: 123 764 162 871 432 747 268 1114
Fragments arranged by size:
1114 871 764 747 432 268 162 123
AflIII A'CryG_T
Cuts at: 1833 2937 3914 1833
Size: 1104 977 2400
Fragments arranged by size:
2400 1104 977
AgeI A'CCGG_T
Cuts at: 2869 2869
Size: 4481
AlwNI CAG_nnn'CTG
Cuts at: 549 843 1786 3353 549
Size: 294 943 1567 1677
Fragments arranged by size:
1677 1567 943 294
ApaI G_GGCC'C
Cuts at: 164 246 409 855 893 1409 1424 164
Size: 82 163 446 38 516 15 3221
Fragments arranged by size:
3221 516 446 163 82 38 15
ApaLI G'TGCA_C
Cuts at: 3251 3251
Size: 4481
ApoI r'AATT_y
Cuts at: 534 1485 3834 4090 534
Size: 951 2349 256 925
Fragments arranged by size:
2349 951 925 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
AvaI C'yCGr_G
Cuts at: 806 1501 806
Size: 695 3786
AvrII C'CTAG_G
Cuts at: 1126 1126
Size: 4481
BamHI G'GATC_C
Cuts at: 1506 1506
Size: 4481
BanI G'GyrC_C
Cuts at: 726 1497 1911 2159 2194 726
Size: 771 414 248 35 3013
Fragments arranged by size:
3013 771 414 248 35
BanII G_rGCy'C
Cuts at: 164 211 246 409 828 855 893 1409 1424
Size: 47 35 163 419 27 38 516 15
Cuts at: 1424 1495 2525 164
Size: 71 1030 2120
Fragments arranged by size:
2120 1030 516 419 163 71 47 38
35 27 15
BbeI G_GCGC'C
Cuts at: 1915 2163 1915
Size: 248 4233
BbsI GAAGACnn'nnnn_
Cuts at: 769 929 769
Size: 160 4321
BbvCI CC'TCA_GC
Cuts at: 746 1280 746
Size: 534 3947
BciVI GTATCCnnnnn_n'
Cuts at: 1093 2379 3140 4481 1093
Size: 1286 761 1341 1093
Fragments arranged by size:
1341 1286 1093 761
BclI T'GATC_A
Cuts at: 2001 2001
Size: 4481
BglI GCCn_nnn'nGGC
Cuts at: 136 250 832 136
Size: 114 582 3785
Fragments arranged by size:
3785 582 114
BglII A'GATC_T
Cuts at: 1996 4033 1996
Size: 2037 2444
BlpI GC'TnA_GC
Cuts at: 1430 1593 1430
Size: 163 4318
Bme1580I G_kGCm'C
Cuts at: 164 246 409 855 893 1409 1424 2106 2199
Size: 82 163 446 38 516 15 682 93
Cuts at: 2199 3255 164
Size: 1056 1390
Fragments arranged by size:
1390 1056 682 516 446 163 93 82
38 15
BmrI ACTGGGnnnn_n'
Cuts at: 136 1412 1693 1774 2109 4253 136
Size: 1276 281 81 335 2144 364
Fragments arranged by size:
2144 1276 364 335 281 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2848
Size: 4481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 1183
Size: 4481
Bpu10I CC'TnA_GC
Cuts at: 746 1280 746
Size: 534 3947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 2536 3028 3326 1657
Size: 879 492 298 2812
Fragments arranged by size:
2812 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1270 4185 1270
Size: 2915 1566
BsaAI yAC'GTr
Cuts at: 1834 1834
Size: 4481
BsaBI GATnn'nnATC
Cuts at: 6 1995 4032 6
Size: 1989 2037 455
Fragments arranged by size:
2037 1989 455
BsaHI Gr'CG_yC
Cuts at: 1912 2160 2862 4358 1912
Size: 248 702 1496 2035
Fragments arranged by size:
2035 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1670 2191 2869 3143 3290 3701 1085
Size: 585 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 585 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 1156 1186 193
Size: 30 933 30 3488
Fragments arranged by size:
3488 933 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 316 319 322 325 1249 183
Size: 133 3 3 3 924 3415
Fragments arranged by size:
3415 924 133 3 3 3
BseYI C'CCAG_C
Cuts at: 266 277 542 741 892 1906 2664 3241 3737
Size: 11 265 199 151 1014 758 577 496
Cuts at: 3737 266
Size: 1010
Fragments arranged by size:
1014 1010 758 577 496 265 199 151
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 887
Size: 4481
BsiEI CG_ry'CG
Cuts at: 104 2023 2069 3277 104
Size: 1919 46 1208 1308
Fragments arranged by size:
1919 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1495 2273 2463 3255 1495
Size: 778 190 792 2721
Fragments arranged by size:
2721 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 916 916
Size: 4481
Bsp1286I G_dGCh'C
Cuts at: 164 211 246 409 828 855 893 1409 1424
Size: 47 35 163 419 27 38 516 15
Cuts at: 1424 1495 2106 2199 2273 2463 2525 3255 164
Size: 71 611 93 74 190 62 730 1390
Fragments arranged by size:
1390 730 611 516 419 190 163 93
74 71 62 47 38 35 27 15
BspEI T'CCGG_A
Cuts at: 1085 3701 1085
Size: 2616 1865
BspHI T'CATG_A
Cuts at: 3657 3657
Size: 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1531 1745 2047 2428 1531
Size: 214 302 381 3584
Fragments arranged by size:
3584 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2773 2827 4311 4476 50
Size: 2723 54 1484 165 55
Fragments arranged by size:
2723 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1098 2393 1098
Size: 1295 3186
BsrFI r'CCGG_y
Cuts at: 677 2479 2660 2869 677
Size: 1802 181 209 2289
Fragments arranged by size:
2289 1802 209 181
BssSI C'ACGA_G
Cuts at: 2752 3110 2752
Size: 358 4123
BstAPI GCAn_nnn'nTGC
Cuts at: 349 1858 349
Size: 1509 2972
BstBI TT'CG_AA
Cuts at: 113 1308 2842 113
Size: 1195 1534 1752
Fragments arranged by size:
1752 1534 1195
BstXI CCAn_nnnn'nTGG
Cuts at: 1402 1402
Size: 4481
BstYI r'GATC_y
Cuts at: 1 1011 1506 1973 1996 2331 2577 3589 3675
Size: 1010 495 467 23 335 246 1012 86
Cuts at: 3675 3687 4033 1
Size: 12 346 449
Fragments arranged by size:
1012 1010 495 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 722 929 722
Size: 207 4274
BtgI C'CryG_G
Cuts at: 149 951 149
Size: 802 3679
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 987 2431 2612 2775 987
Size: 1444 181 163 2693
Fragments arranged by size:
2693 1444 181 163
BtsI GCAGTG_nn'
Cuts at: 423 483 673 903 982 1724 423
Size: 60 190 230 79 742 3180
Fragments arranged by size:
3180 742 230 190 79 60
DraI TTT'AAA
Cuts at: 1480 1480
Size: 4481
DraIII CAC_nnn'GTG
Cuts at: 1139 1139
Size: 4481
DrdI GACnn_nn'nnGTC
Cuts at: 771 1129 2187 3045 771
Size: 358 1058 858 2207
Fragments arranged by size:
2207 1058 858 358
EaeI y'GGCC_r
Cuts at: 230 979 2066 2240 2631 2658 4003 230
Size: 749 1087 174 391 27 1345 708
Fragments arranged by size:
1345 1087 749 708 391 174 27
EagI C'GGCC_G
Cuts at: 2066 2066
Size: 4481
EarI CTCTTCn'nnn_
Cuts at: 110 919 2504 2714 110
Size: 809 1585 210 1877
Fragments arranged by size:
1877 1585 809 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 170 687 2689 2999 3145 4291 170
Size: 517 2002 310 146 1146 360
Fragments arranged by size:
2002 1146 517 360 310 146
EcoICRI GAG'CTC
Cuts at: 1493 1493
Size: 4481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 509 1183 1273 1435 2306 2738 3485 3753
Size: 123 674 90 162 871 432 747 268
Cuts at: 3753 386
Size: 1114
Fragments arranged by size:
1114 871 747 674 432 268 162 123
90
EcoNI CCTnn'n_nnAGG
Cuts at: 1234 1415 1234
Size: 181 4300
EcoO109I rG'GnC_Cy
Cuts at: 243 405 571 738 750 851 889 1359 1406
Size: 162 166 167 12 101 38 470 47
Cuts at: 1406 1420 1620 243
Size: 14 200 3104
Fragments arranged by size:
3104 470 200 167 166 162 101 47
38 14 12
EcoRI G'AATT_C
Cuts at: 1485 1485
Size: 4481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1356 2500 1356
Size: 1144 3337
FspI TGC'GCA
Cuts at: 2262 2262
Size: 4481
HaeII r_GCGC'y
Cuts at: 219 1915 2163 3185 4164 219
Size: 1696 248 1022 979 536
Fragments arranged by size:
1696 1022 979 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 1156 1188 2323 2353 2355 2385 241
Size: 32 883 32 1135 30 2 30 2337
Fragments arranged by size:
2337 1135 883 32 32 30 30 2
HincII GTy'rAC
Cuts at: 561 1520 561
Size: 959 3522
KasI G'GCGC_C
Cuts at: 1911 2159 1911
Size: 248 4233
KpnI G_GTAC'C
Cuts at: 1501 1501
Size: 4481
MluI A'CGCG_T
Cuts at: 3914 3914
Size: 4481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3152 3336 3152
Size: 184 4297
MscI TGG'CCA
Cuts at: 232 981 2242 232
Size: 749 1261 2471
Fragments arranged by size:
2471 1261 749
MslI CAynn'nnrTG
Cuts at: 2470 2597 2470
Size: 127 4354
MspA1I CmG'CkG
Cuts at: 151 745 840 953 1023 1592 1906 2266 3279
Size: 594 95 113 70 569 314 360 1013
Cuts at: 3279 3524 151
Size: 245 1108
Fragments arranged by size:
1108 1013 594 569 360 314 245 113
95 70
NaeI GCC'GGC
Cuts at: 2662 2662
Size: 4481
NarI GG'CG_CC
Cuts at: 1912 2160 1912
Size: 248 4233
NgoMIV G'CCGG_C
Cuts at: 2660 2660
Size: 4481
NspI r_CATG'y
Cuts at: 526 1534 2034 2941 526
Size: 1008 500 907 2066
Fragments arranged by size:
2066 1008 907 500
PasI CC'CwG_GG
Cuts at: 622 622
Size: 4481
PciI A'CATG_T
Cuts at: 2937 2937
Size: 4481
PflMI CCAn_nnn'nTGG
Cuts at: 228 549 581 228
Size: 321 32 4128
Fragments arranged by size:
4128 321 32
PfoI T'CCnGG_A
Cuts at: 917 917
Size: 4481
PmeI GTTT'AAAC
Cuts at: 1480 1480
Size: 4481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4449
PpuMI rG'GwC_Cy
Cuts at: 571 1359 571
Size: 788 3693
PspOMI G'GGCC_C
Cuts at: 160 242 405 851 889 1405 1420 160
Size: 82 163 446 38 516 15 3221
Fragments arranged by size:
3221 516 446 163 82 38 15
PstI C_TGCA'G
Cuts at: 989 1448 1528 2213 989
Size: 459 80 685 3257
Fragments arranged by size:
3257 685 459 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4481
PvuII CAG'CTG
Cuts at: 745 1906 2266 745
Size: 1161 360 2960
Fragments arranged by size:
2960 1161 360
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 4481
SacI G_AGCT'C
Cuts at: 1495 1495
Size: 4481
SacII CC_GC'GG
Cuts at: 152 954 152
Size: 802 3679
SalI G'TCGA_C
Cuts at: 1518 1518
Size: 4481
SapI GCTCTTCn'nnn_
Cuts at: 110 2504 2714 110
Size: 2394 210 1877
Fragments arranged by size:
2394 1877 210
SbfI CC_TGCA'GG
Cuts at: 1528 1528
Size: 4481
ScaI AGT'ACT
Cuts at: 594 1250 3998 594
Size: 656 2748 1077
Fragments arranged by size:
2748 1077 656
SfcI C'TryA_G
Cuts at: 33 985 1444 1524 2209 3202 3393 33
Size: 952 459 80 685 993 191 1121
Fragments arranged by size:
1121 993 952 685 459 191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 136 136
Size: 4481
SfoI GGC'GCC
Cuts at: 1913 2161 1913
Size: 248 4233
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
SmaI CCC'GGG
Cuts at: 1503 1503
Size: 4481
SmlI C'TyrA_G
Cuts at: 1636 2551 3043 3305 1636
Size: 915 492 262 2812
Fragments arranged by size:
2812 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4481
SphI G_CATG'C
Cuts at: 1534 2034 1534
Size: 500 3981
StuI AGG'CCT
Cuts at: 1442 1442
Size: 4481
StyI C'CwwG_G
Cuts at: 156 1126 1355 1424 1615 1969 156
Size: 970 229 69 191 354 2668
Fragments arranged by size:
2668 970 354 229 191 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2064 2197 2656 2865 2064
Size: 133 459 209 3680
Fragments arranged by size:
3680 459 209 133
TatI w'GTAC_w
Cuts at: 592 881 1248 3996 592
Size: 289 367 2748 1077
Fragments arranged by size:
2748 1077 367 289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 4449
Tth111I GACn'n_nGTC
Cuts at: 2278 2278
Size: 4481
XbaI T'CTAG_A
Cuts at: 1512 1512
Size: 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1976 1976
Size: 4481
XmaI C'CCGG_G
Cuts at: 1501 1501
Size: 4481
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI